id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-016858-pbjj50bx	Jung, Kwonil	Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues	2015-09-10		.txt	text/plain	2133	153	53	PEDV antigens in frozen tissues are visualized as green staining in the cytoplasm of infected cells by fluorescent dyes conjugated with antibodies when activated by exciting light of a specific wavelength under a fluorescence microscope. In FFPE tissues, PEDV antigens are visualized as red staining in the cytoplasm of infected cells by the deposition of the substrate chromogen, Fast Red. 2013 to present, has led to a substantial loss of piglets (more than 10 % of US swine population). Viral antigens in frozen, fi xed cells, or tissues can be detected by immunohistochemistry (IHC) (or immunohistochemical staining) using specifi c antibodies labeled with fl uorescent dyes, such as Alexa Fluor ® 488 and fl uorescein isothiocyanate (FITC), or enzymes, such as alkaline phosphatase (AP) and peroxidase. Monoclonal antibody-based immunohistochemical detection of porcine epidemic diarrhea virus antigen in formalin-fi xed, paraffi n-embedded intestinal tissues	./cache/cord-016858-pbjj50bx.txt	./txt/cord-016858-pbjj50bx.txt