id sid tid token lemma pos cord-279980-49yv65gm 1 1 key key NN cord-279980-49yv65gm 1 2 : : : cord-279980-49yv65gm 2 1 cord-279980 cord-279980 NNP cord-279980-49yv65gm 2 2 - - HYPH cord-279980-49yv65gm 2 3 49yv65gm 49yv65gm CD cord-279980-49yv65gm 2 4 authors author NNS cord-279980-49yv65gm 2 5 : : : cord-279980-49yv65gm 2 6 WANARATANA WANARATANA NNP cord-279980-49yv65gm 2 7 , , , cord-279980-49yv65gm 2 8 S. S. NNP cord-279980-49yv65gm 2 9 ; ; : cord-279980-49yv65gm 2 10 PANYIM PANYIM NNP cord-279980-49yv65gm 2 11 , , , cord-279980-49yv65gm 2 12 S. S. NNP cord-279980-49yv65gm 2 13 ; ; : cord-279980-49yv65gm 2 14 PAKPINYO PAKPINYO NNP cord-279980-49yv65gm 2 15 , , , cord-279980-49yv65gm 2 16 S. S. NNP cord-279980-49yv65gm 2 17 title title NN cord-279980-49yv65gm 2 18 : : : cord-279980-49yv65gm 3 1 The the DT cord-279980-49yv65gm 3 2 potential potential NN cord-279980-49yv65gm 3 3 of of IN cord-279980-49yv65gm 3 4 house house NN cord-279980-49yv65gm 3 5 flies fly VBZ cord-279980-49yv65gm 3 6 to to TO cord-279980-49yv65gm 3 7 act act VB cord-279980-49yv65gm 3 8 as as IN cord-279980-49yv65gm 3 9 a a DT cord-279980-49yv65gm 3 10 vector vector NN cord-279980-49yv65gm 3 11 of of IN cord-279980-49yv65gm 3 12 avian avian JJ cord-279980-49yv65gm 3 13 influenza influenza NN cord-279980-49yv65gm 3 14 subtype subtype NN cord-279980-49yv65gm 3 15 H5N1 H5N1 NNP cord-279980-49yv65gm 3 16 under under IN cord-279980-49yv65gm 3 17 experimental experimental JJ cord-279980-49yv65gm 3 18 conditions condition NNS cord-279980-49yv65gm 3 19 date date NN cord-279980-49yv65gm 4 1 : : : cord-279980-49yv65gm 4 2 2010 2010 CD cord-279980-49yv65gm 4 3 - - SYM cord-279980-49yv65gm 4 4 12 12 CD cord-279980-49yv65gm 4 5 - - HYPH cord-279980-49yv65gm 4 6 01 01 CD cord-279980-49yv65gm 4 7 journal journal NN cord-279980-49yv65gm 4 8 : : : cord-279980-49yv65gm 5 1 Med Med NNP cord-279980-49yv65gm 5 2 Vet Vet NNP cord-279980-49yv65gm 5 3 Entomol Entomol NNP cord-279980-49yv65gm 6 1 DOI DOI NNP cord-279980-49yv65gm 6 2 : : : cord-279980-49yv65gm 7 1 10.1111 10.1111 CD cord-279980-49yv65gm 7 2 / / SYM cord-279980-49yv65gm 7 3 j.1365 j.1365 XX cord-279980-49yv65gm 7 4 - - HYPH cord-279980-49yv65gm 7 5 2915.2010.00928.x 2915.2010.00928.x CD cord-279980-49yv65gm 7 6 sha sha NNP cord-279980-49yv65gm 7 7 : : : cord-279980-49yv65gm 7 8 4344fb4d3763d67b29fb70643a18d1f843528f39 4344fb4d3763d67b29fb70643a18d1f843528f39 CD cord-279980-49yv65gm 7 9 doc_id doc_id CD cord-279980-49yv65gm 7 10 : : : cord-279980-49yv65gm 7 11 279980 279980 CD cord-279980-49yv65gm 7 12 cord_uid cord_uid NNS cord-279980-49yv65gm 7 13 : : : cord-279980-49yv65gm 8 1 49yv65gm 49yv65gm LS cord-279980-49yv65gm 9 1 The the DT cord-279980-49yv65gm 9 2 objective objective NN cord-279980-49yv65gm 9 3 of of IN cord-279980-49yv65gm 9 4 the the DT cord-279980-49yv65gm 9 5 present present JJ cord-279980-49yv65gm 9 6 study study NN cord-279980-49yv65gm 9 7 was be VBD cord-279980-49yv65gm 9 8 to to TO cord-279980-49yv65gm 9 9 determine determine VB cord-279980-49yv65gm 9 10 the the DT cord-279980-49yv65gm 9 11 potential potential NN cord-279980-49yv65gm 9 12 for for IN cord-279980-49yv65gm 9 13 house house NN cord-279980-49yv65gm 9 14 flies fly NNS cord-279980-49yv65gm 9 15 ( ( -LRB- cord-279980-49yv65gm 9 16 Musca Musca NNP cord-279980-49yv65gm 9 17 domestica domestica NNP cord-279980-49yv65gm 9 18 L. L. NNP cord-279980-49yv65gm 9 19 ) ) -RRB- cord-279980-49yv65gm 10 1 ( ( -LRB- cord-279980-49yv65gm 10 2 Diptera Diptera NNP cord-279980-49yv65gm 10 3 : : : cord-279980-49yv65gm 10 4 Muscidae Muscidae NNP cord-279980-49yv65gm 10 5 ) ) -RRB- cord-279980-49yv65gm 11 1 to to TO cord-279980-49yv65gm 11 2 harbour harbour VB cord-279980-49yv65gm 11 3 the the DT cord-279980-49yv65gm 11 4 avian avian JJ cord-279980-49yv65gm 11 5 influenza influenza NN cord-279980-49yv65gm 11 6 ( ( -LRB- cord-279980-49yv65gm 11 7 AI AI NNP cord-279980-49yv65gm 11 8 ) ) -RRB- cord-279980-49yv65gm 11 9 H5N1 H5N1 NNP cord-279980-49yv65gm 11 10 virus virus NN cord-279980-49yv65gm 11 11 . . . cord-279980-49yv65gm 12 1 Laboratory‐reared laboratory‐reare VBN cord-279980-49yv65gm 12 2 flies fly NNS cord-279980-49yv65gm 12 3 were be VBD cord-279980-49yv65gm 12 4 experimentally experimentally RB cord-279980-49yv65gm 12 5 fed feed VBN cord-279980-49yv65gm 12 6 with with IN cord-279980-49yv65gm 12 7 a a DT cord-279980-49yv65gm 12 8 mixture mixture NN cord-279980-49yv65gm 12 9 containing contain VBG cord-279980-49yv65gm 12 10 the the DT cord-279980-49yv65gm 12 11 AI AI NNP cord-279980-49yv65gm 12 12 virus virus NN cord-279980-49yv65gm 12 13 . . . cord-279980-49yv65gm 13 1 Exposed expose VBN cord-279980-49yv65gm 13 2 flies fly NNS cord-279980-49yv65gm 13 3 were be VBD cord-279980-49yv65gm 13 4 washed wash VBN cord-279980-49yv65gm 13 5 with with IN cord-279980-49yv65gm 13 6 brain brain NN cord-279980-49yv65gm 13 7 – – : cord-279980-49yv65gm 13 8 heart heart NN cord-279980-49yv65gm 13 9 infusion infusion NN cord-279980-49yv65gm 13 10 broth broth NN cord-279980-49yv65gm 13 11 and and CC cord-279980-49yv65gm 13 12 followed follow VBN cord-279980-49yv65gm 13 13 by by IN cord-279980-49yv65gm 13 14 70 70 CD cord-279980-49yv65gm 13 15 % % NN cord-279980-49yv65gm 13 16 alcohol alcohol NN cord-279980-49yv65gm 13 17 before before IN cord-279980-49yv65gm 13 18 preparation preparation NN cord-279980-49yv65gm 13 19 of of IN cord-279980-49yv65gm 13 20 whole whole JJ cord-279980-49yv65gm 13 21 fly fly NN cord-279980-49yv65gm 13 22 homogenate homogenate NNP cord-279980-49yv65gm 13 23 . . . cord-279980-49yv65gm 14 1 The the DT cord-279980-49yv65gm 14 2 homogenate homogenate NNP cord-279980-49yv65gm 14 3 was be VBD cord-279980-49yv65gm 14 4 inoculated inoculate VBN cord-279980-49yv65gm 14 5 into into IN cord-279980-49yv65gm 14 6 six six CD cord-279980-49yv65gm 14 7 10‐day‐old 10‐day‐old CD cord-279980-49yv65gm 14 8 embryonated embryonate VBN cord-279980-49yv65gm 14 9 chicken chicken NN cord-279980-49yv65gm 14 10 eggs egg NNS cord-279980-49yv65gm 14 11 ( ( -LRB- cord-279980-49yv65gm 14 12 ECEs ECEs NNP cord-279980-49yv65gm 14 13 ) ) -RRB- cord-279980-49yv65gm 14 14 . . . cord-279980-49yv65gm 15 1 Allantoic Allantoic NNP cord-279980-49yv65gm 15 2 fluids fluid NNS cord-279980-49yv65gm 15 3 were be VBD cord-279980-49yv65gm 15 4 collected collect VBN cord-279980-49yv65gm 15 5 to to TO cord-279980-49yv65gm 15 6 determine determine VB cord-279980-49yv65gm 15 7 the the DT cord-279980-49yv65gm 15 8 virus virus NN cord-279980-49yv65gm 15 9 using use VBG cord-279980-49yv65gm 15 10 the the DT cord-279980-49yv65gm 15 11 haemagglutination haemagglutination NN cord-279980-49yv65gm 15 12 ( ( -LRB- cord-279980-49yv65gm 15 13 HA HA NNP cord-279980-49yv65gm 15 14 ) ) -RRB- cord-279980-49yv65gm 15 15 test test NN cord-279980-49yv65gm 15 16 , , , cord-279980-49yv65gm 15 17 reverse reverse VB cord-279980-49yv65gm 15 18 transcription‐polymerase transcription‐polymerase JJ cord-279980-49yv65gm 15 19 chain chain NN cord-279980-49yv65gm 15 20 reaction reaction NN cord-279980-49yv65gm 15 21 ( ( -LRB- cord-279980-49yv65gm 15 22 RT‐PCR RT‐PCR NNP cord-279980-49yv65gm 15 23 ) ) -RRB- cord-279980-49yv65gm 15 24 or or CC cord-279980-49yv65gm 15 25 quantitative quantitative JJ cord-279980-49yv65gm 15 26 real‐time real‐time NN cord-279980-49yv65gm 15 27 RT‐PCR rt‐pcr XX cord-279980-49yv65gm 15 28 ( ( -LRB- cord-279980-49yv65gm 15 29 RRT‐PCR RRT‐PCR NNP cord-279980-49yv65gm 15 30 ) ) -RRB- cord-279980-49yv65gm 15 31 . . . cord-279980-49yv65gm 16 1 In in IN cord-279980-49yv65gm 16 2 the the DT cord-279980-49yv65gm 16 3 first first JJ cord-279980-49yv65gm 16 4 experiment experiment NN cord-279980-49yv65gm 16 5 , , , cord-279980-49yv65gm 16 6 ECEs ECEs NNP cord-279980-49yv65gm 16 7 that that WDT cord-279980-49yv65gm 16 8 were be VBD cord-279980-49yv65gm 16 9 inoculated inoculate VBN cord-279980-49yv65gm 16 10 with with IN cord-279980-49yv65gm 16 11 the the DT cord-279980-49yv65gm 16 12 50 50 CD cord-279980-49yv65gm 16 13 AI AI NNP cord-279980-49yv65gm 16 14 virus virus NN cord-279980-49yv65gm 16 15 exposed expose VBN cord-279980-49yv65gm 16 16 fly fly NN cord-279980-49yv65gm 16 17 homogenates homogenate NNS cord-279980-49yv65gm 16 18 died die VBD cord-279980-49yv65gm 16 19 within within IN cord-279980-49yv65gm 16 20 48 48 CD cord-279980-49yv65gm 16 21 h h NN cord-279980-49yv65gm 16 22 and and CC cord-279980-49yv65gm 16 23 HA HA NNP cord-279980-49yv65gm 16 24 and and CC cord-279980-49yv65gm 16 25 RT‐PCR RT‐PCR NNP cord-279980-49yv65gm 16 26 were be VBD cord-279980-49yv65gm 16 27 positive positive JJ cord-279980-49yv65gm 16 28 for for IN cord-279980-49yv65gm 16 29 AI AI NNP cord-279980-49yv65gm 16 30 virus virus NN cord-279980-49yv65gm 16 31 . . . cord-279980-49yv65gm 17 1 In in IN cord-279980-49yv65gm 17 2 the the DT cord-279980-49yv65gm 17 3 second second JJ cord-279980-49yv65gm 17 4 experiment experiment NN cord-279980-49yv65gm 17 5 , , , cord-279980-49yv65gm 17 6 ECEs ECEs NNP cord-279980-49yv65gm 17 7 that that WDT cord-279980-49yv65gm 17 8 were be VBD cord-279980-49yv65gm 17 9 inoculated inoculate VBN cord-279980-49yv65gm 17 10 with with IN cord-279980-49yv65gm 17 11 only only RB cord-279980-49yv65gm 17 12 one one CD cord-279980-49yv65gm 17 13 fly fly NN cord-279980-49yv65gm 17 14 died die VBD cord-279980-49yv65gm 17 15 with with IN cord-279980-49yv65gm 17 16 positive positive JJ cord-279980-49yv65gm 17 17 HA HA NNP cord-279980-49yv65gm 17 18 test test NN cord-279980-49yv65gm 17 19 and and CC cord-279980-49yv65gm 17 20 RT‐PCR rt‐pcr ADD cord-279980-49yv65gm 17 21 . . . cord-279980-49yv65gm 18 1 In in IN cord-279980-49yv65gm 18 2 the the DT cord-279980-49yv65gm 18 3 last last JJ cord-279980-49yv65gm 18 4 experiment experiment NN cord-279980-49yv65gm 18 5 , , , cord-279980-49yv65gm 18 6 a a DT cord-279980-49yv65gm 18 7 group group NN cord-279980-49yv65gm 18 8 of of IN cord-279980-49yv65gm 18 9 exposed expose VBN cord-279980-49yv65gm 18 10 flies fly NNS cord-279980-49yv65gm 18 11 was be VBD cord-279980-49yv65gm 18 12 collected collect VBN cord-279980-49yv65gm 18 13 at at IN cord-279980-49yv65gm 18 14 0 0 CD cord-279980-49yv65gm 18 15 , , , cord-279980-49yv65gm 18 16 6 6 CD cord-279980-49yv65gm 18 17 , , , cord-279980-49yv65gm 18 18 12 12 CD cord-279980-49yv65gm 18 19 , , , cord-279980-49yv65gm 18 20 24 24 CD cord-279980-49yv65gm 18 21 , , , cord-279980-49yv65gm 18 22 36 36 CD cord-279980-49yv65gm 18 23 , , , cord-279980-49yv65gm 18 24 48 48 CD cord-279980-49yv65gm 18 25 , , , cord-279980-49yv65gm 18 26 72 72 CD cord-279980-49yv65gm 18 27 and and CC cord-279980-49yv65gm 18 28 96 96 CD cord-279980-49yv65gm 18 29 h h NN cord-279980-49yv65gm 18 30 post‐exposure post‐exposure NN cord-279980-49yv65gm 18 31 . . . cord-279980-49yv65gm 19 1 Fly fly VB cord-279980-49yv65gm 19 2 homogenates homogenate NNS cord-279980-49yv65gm 19 3 of of IN cord-279980-49yv65gm 19 4 each each DT cord-279980-49yv65gm 19 5 time time NN cord-279980-49yv65gm 19 6 point point NN cord-279980-49yv65gm 19 7 were be VBD cord-279980-49yv65gm 19 8 tested test VBN cord-279980-49yv65gm 19 9 by by IN cord-279980-49yv65gm 19 10 virus virus NN cord-279980-49yv65gm 19 11 titration titration NN cord-279980-49yv65gm 19 12 in in IN cord-279980-49yv65gm 19 13 ECEs ECEs NNP cord-279980-49yv65gm 19 14 and and CC cord-279980-49yv65gm 19 15 RRT‐PCR RRT‐PCR NNP cord-279980-49yv65gm 19 16 . . . cord-279980-49yv65gm 20 1 Virus virus NN cord-279980-49yv65gm 20 2 titres titre NNS cord-279980-49yv65gm 20 3 declined decline VBD cord-279980-49yv65gm 20 4 in in IN cord-279980-49yv65gm 20 5 relation relation NN cord-279980-49yv65gm 20 6 to to IN cord-279980-49yv65gm 20 7 exposure exposure NN cord-279980-49yv65gm 20 8 time time NN cord-279980-49yv65gm 20 9 . . . cord-279980-49yv65gm 21 1 Furthermore furthermore RB cord-279980-49yv65gm 21 2 , , , cord-279980-49yv65gm 21 3 RRT‐PCR RRT‐PCR NNP cord-279980-49yv65gm 21 4 results result NNS cord-279980-49yv65gm 21 5 were be VBD cord-279980-49yv65gm 21 6 positive positive JJ cord-279980-49yv65gm 21 7 at at IN cord-279980-49yv65gm 21 8 any any DT cord-279980-49yv65gm 21 9 time time NN cord-279980-49yv65gm 21 10 point point NN cord-279980-49yv65gm 21 11 . . . cord-279980-49yv65gm 22 1 The the DT cord-279980-49yv65gm 22 2 present present JJ cord-279980-49yv65gm 22 3 study study NN cord-279980-49yv65gm 22 4 shows show VBZ cord-279980-49yv65gm 22 5 that that IN cord-279980-49yv65gm 22 6 the the DT cord-279980-49yv65gm 22 7 flies fly NNS cord-279980-49yv65gm 22 8 may may MD cord-279980-49yv65gm 22 9 harbour harbour VB cord-279980-49yv65gm 22 10 the the DT cord-279980-49yv65gm 22 11 AI AI NNP cord-279980-49yv65gm 22 12 virus virus NN cord-279980-49yv65gm 22 13 and and CC cord-279980-49yv65gm 22 14 could could MD cord-279980-49yv65gm 22 15 act act VB cord-279980-49yv65gm 22 16 as as IN cord-279980-49yv65gm 22 17 a a DT cord-279980-49yv65gm 22 18 mechanical mechanical JJ cord-279980-49yv65gm 22 19 vector vector NN cord-279980-49yv65gm 22 20 of of IN cord-279980-49yv65gm 22 21 the the DT cord-279980-49yv65gm 22 22 AI AI NNP cord-279980-49yv65gm 22 23 virus virus NN cord-279980-49yv65gm 22 24 . . . cord-279980-49yv65gm 23 1 Avian avian JJ cord-279980-49yv65gm 23 2 influenza influenza NN cord-279980-49yv65gm 23 3 ( ( -LRB- cord-279980-49yv65gm 23 4 AI AI NNP cord-279980-49yv65gm 23 5 ) ) -RRB- cord-279980-49yv65gm 23 6 is be VBZ cord-279980-49yv65gm 23 7 a a DT cord-279980-49yv65gm 23 8 highly highly RB cord-279980-49yv65gm 23 9 contagious contagious JJ cord-279980-49yv65gm 23 10 disease disease NN cord-279980-49yv65gm 23 11 caused cause VBN cord-279980-49yv65gm 23 12 by by IN cord-279980-49yv65gm 23 13 the the DT cord-279980-49yv65gm 23 14 type type NN cord-279980-49yv65gm 23 15 A a DT cord-279980-49yv65gm 23 16 influenza influenza NN cord-279980-49yv65gm 23 17 virus virus NN cord-279980-49yv65gm 23 18 of of IN cord-279980-49yv65gm 23 19 the the DT cord-279980-49yv65gm 23 20 family family NN cord-279980-49yv65gm 23 21 Orthomyxoviridae Orthomyxoviridae NNP cord-279980-49yv65gm 23 22 . . . cord-279980-49yv65gm 24 1 Highly highly RB cord-279980-49yv65gm 24 2 pathogenic pathogenic JJ cord-279980-49yv65gm 24 3 avian avian JJ cord-279980-49yv65gm 24 4 influenza influenza NN cord-279980-49yv65gm 24 5 ( ( -LRB- cord-279980-49yv65gm 24 6 HPAI HPAI NNP cord-279980-49yv65gm 24 7 ) ) -RRB- cord-279980-49yv65gm 24 8 viruses virus NNS cord-279980-49yv65gm 24 9 , , , cord-279980-49yv65gm 24 10 especially especially RB cord-279980-49yv65gm 24 11 subtype subtype NN cord-279980-49yv65gm 24 12 H5N1 H5N1 NNP cord-279980-49yv65gm 24 13 , , , cord-279980-49yv65gm 24 14 cause cause VB cord-279980-49yv65gm 24 15 a a DT cord-279980-49yv65gm 24 16 multi multi JJ cord-279980-49yv65gm 24 17 - - JJ cord-279980-49yv65gm 24 18 systemic systemic JJ cord-279980-49yv65gm 24 19 disease disease NN cord-279980-49yv65gm 24 20 associated associate VBN cord-279980-49yv65gm 24 21 with with IN cord-279980-49yv65gm 24 22 high high JJ cord-279980-49yv65gm 24 23 morbidity morbidity NN cord-279980-49yv65gm 24 24 and and CC cord-279980-49yv65gm 24 25 mortality mortality NN cord-279980-49yv65gm 24 26 in in IN cord-279980-49yv65gm 24 27 poultry poultry NN cord-279980-49yv65gm 24 28 , , , cord-279980-49yv65gm 24 29 as as RB cord-279980-49yv65gm 24 30 well well RB cord-279980-49yv65gm 24 31 as as IN cord-279980-49yv65gm 24 32 in in IN cord-279980-49yv65gm 24 33 mammals mammal NNS cord-279980-49yv65gm 24 34 and and CC cord-279980-49yv65gm 24 35 humans human NNS cord-279980-49yv65gm 24 36 ( ( -LRB- cord-279980-49yv65gm 24 37 Mahy Mahy NNP cord-279980-49yv65gm 24 38 & & CC cord-279980-49yv65gm 24 39 Van Van NNP cord-279980-49yv65gm 24 40 Regenmortel Regenmortel NNP cord-279980-49yv65gm 24 41 , , , cord-279980-49yv65gm 24 42 2008 2008 CD cord-279980-49yv65gm 24 43 ) ) -RRB- cord-279980-49yv65gm 24 44 . . . cord-279980-49yv65gm 25 1 The the DT cord-279980-49yv65gm 25 2 emergence emergence NN cord-279980-49yv65gm 25 3 of of IN cord-279980-49yv65gm 25 4 the the DT cord-279980-49yv65gm 25 5 AI AI NNP cord-279980-49yv65gm 25 6 H5N1 H5N1 NNP cord-279980-49yv65gm 25 7 virus virus NN cord-279980-49yv65gm 25 8 was be VBD cord-279980-49yv65gm 25 9 first first RB cord-279980-49yv65gm 25 10 reported report VBN cord-279980-49yv65gm 25 11 in in IN cord-279980-49yv65gm 25 12 1996 1996 CD cord-279980-49yv65gm 25 13 in in IN cord-279980-49yv65gm 25 14 mainland mainland NN cord-279980-49yv65gm 25 15 China China NNP cord-279980-49yv65gm 26 1 ( ( -LRB- cord-279980-49yv65gm 26 2 Chen Chen NNP cord-279980-49yv65gm 26 3 et et NNP cord-279980-49yv65gm 26 4 al al NNP cord-279980-49yv65gm 26 5 . . NNP cord-279980-49yv65gm 26 6 , , , cord-279980-49yv65gm 26 7 2004 2004 CD cord-279980-49yv65gm 26 8 ) ) -RRB- cord-279980-49yv65gm 26 9 and and CC cord-279980-49yv65gm 26 10 later later RB cord-279980-49yv65gm 26 11 spread spread VBD cord-279980-49yv65gm 26 12 to to TO cord-279980-49yv65gm 26 13 live live VB cord-279980-49yv65gm 26 14 poultry poultry NN cord-279980-49yv65gm 26 15 markets market NNS cord-279980-49yv65gm 26 16 in in IN cord-279980-49yv65gm 26 17 Hong Hong NNP cord-279980-49yv65gm 26 18 Kong Kong NNP cord-279980-49yv65gm 27 1 ( ( -LRB- cord-279980-49yv65gm 27 2 Sims Sims NNP cord-279980-49yv65gm 27 3 et et NNP cord-279980-49yv65gm 27 4 al al NNP cord-279980-49yv65gm 27 5 . . . cord-279980-49yv65gm 28 1 , , , cord-279980-49yv65gm 28 2 2005 2005 CD cord-279980-49yv65gm 28 3 ) ) -RRB- cord-279980-49yv65gm 28 4 . . . cord-279980-49yv65gm 29 1 In in IN cord-279980-49yv65gm 29 2 early early JJ cord-279980-49yv65gm 29 3 2004 2004 CD cord-279980-49yv65gm 29 4 , , , cord-279980-49yv65gm 29 5 AI AI NNP cord-279980-49yv65gm 29 6 H5N1 H5N1 NNP cord-279980-49yv65gm 29 7 virus virus NN cord-279980-49yv65gm 29 8 outbreaks outbreak NNS cord-279980-49yv65gm 29 9 were be VBD cord-279980-49yv65gm 29 10 reported report VBN cord-279980-49yv65gm 29 11 in in IN cord-279980-49yv65gm 29 12 several several JJ cord-279980-49yv65gm 29 13 Asian asian JJ cord-279980-49yv65gm 29 14 countries country NNS cord-279980-49yv65gm 29 15 including include VBG cord-279980-49yv65gm 29 16 Thailand Thailand NNP cord-279980-49yv65gm 29 17 . . . cord-279980-49yv65gm 30 1 Since since IN cord-279980-49yv65gm 30 2 then then RB cord-279980-49yv65gm 30 3 , , , cord-279980-49yv65gm 30 4 the the DT cord-279980-49yv65gm 30 5 viruses virus NNS cord-279980-49yv65gm 30 6 have have VBP cord-279980-49yv65gm 30 7 spread spread VBN cord-279980-49yv65gm 30 8 to to IN cord-279980-49yv65gm 30 9 many many JJ cord-279980-49yv65gm 30 10 areas area NNS cord-279980-49yv65gm 30 11 around around IN cord-279980-49yv65gm 30 12 the the DT cord-279980-49yv65gm 30 13 world world NN cord-279980-49yv65gm 30 14 , , , cord-279980-49yv65gm 30 15 causing cause VBG cord-279980-49yv65gm 30 16 major major JJ cord-279980-49yv65gm 30 17 economic economic JJ cord-279980-49yv65gm 30 18 loss loss NN cord-279980-49yv65gm 30 19 in in IN cord-279980-49yv65gm 30 20 poultry poultry NN cord-279980-49yv65gm 30 21 industries industry NNS cord-279980-49yv65gm 30 22 ( ( -LRB- cord-279980-49yv65gm 30 23 OIE OIE NNP cord-279980-49yv65gm 30 24 , , , cord-279980-49yv65gm 30 25 2008 2008 CD cord-279980-49yv65gm 30 26 ) ) -RRB- cord-279980-49yv65gm 30 27 . . . cord-279980-49yv65gm 31 1 Moreover moreover RB cord-279980-49yv65gm 31 2 , , , cord-279980-49yv65gm 31 3 the the DT cord-279980-49yv65gm 31 4 impact impact NN cord-279980-49yv65gm 31 5 from from IN cord-279980-49yv65gm 31 6 AI AI NNP cord-279980-49yv65gm 31 7 H5N1 H5N1 NNP cord-279980-49yv65gm 31 8 virus virus NN cord-279980-49yv65gm 31 9 outbreaks outbreak NNS cord-279980-49yv65gm 31 10 is be VBZ cord-279980-49yv65gm 31 11 not not RB cord-279980-49yv65gm 31 12 limited limit VBN cord-279980-49yv65gm 31 13 to to IN cord-279980-49yv65gm 31 14 poultry poultry NN cord-279980-49yv65gm 31 15 species specie NNS cord-279980-49yv65gm 31 16 as as IN cord-279980-49yv65gm 31 17 the the DT cord-279980-49yv65gm 31 18 virus virus NN cord-279980-49yv65gm 31 19 also also RB cord-279980-49yv65gm 31 20 infects infect VBZ cord-279980-49yv65gm 31 21 other other JJ cord-279980-49yv65gm 31 22 animals animal NNS cord-279980-49yv65gm 31 23 and and CC cord-279980-49yv65gm 31 24 humans human NNS cord-279980-49yv65gm 31 25 . . . cord-279980-49yv65gm 32 1 As as IN cord-279980-49yv65gm 32 2 of of IN cord-279980-49yv65gm 32 3 September September NNP cord-279980-49yv65gm 32 4 2009 2009 CD cord-279980-49yv65gm 32 5 , , , cord-279980-49yv65gm 32 6 there there EX cord-279980-49yv65gm 32 7 are be VBP cord-279980-49yv65gm 32 8 more more JJR cord-279980-49yv65gm 32 9 than than IN cord-279980-49yv65gm 32 10 442 442 CD cord-279980-49yv65gm 32 11 human human JJ cord-279980-49yv65gm 32 12 cases case NNS cord-279980-49yv65gm 32 13 with with IN cord-279980-49yv65gm 32 14 262 262 CD cord-279980-49yv65gm 32 15 fatal fatal JJ cord-279980-49yv65gm 32 16 cases case NNS cord-279980-49yv65gm 32 17 . . . cord-279980-49yv65gm 33 1 This this DT cord-279980-49yv65gm 33 2 leads lead VBZ cord-279980-49yv65gm 33 3 to to IN cord-279980-49yv65gm 33 4 a a DT cord-279980-49yv65gm 33 5 serious serious JJ cord-279980-49yv65gm 33 6 concern concern NN cord-279980-49yv65gm 33 7 regarding regard VBG cord-279980-49yv65gm 33 8 the the DT cord-279980-49yv65gm 33 9 pandemic pandemic NN cord-279980-49yv65gm 33 10 potential potential NN cord-279980-49yv65gm 33 11 of of IN cord-279980-49yv65gm 33 12 the the DT cord-279980-49yv65gm 33 13 AI AI NNP cord-279980-49yv65gm 33 14 H5N1 H5N1 NNP cord-279980-49yv65gm 33 15 virus virus NN cord-279980-49yv65gm 33 16 ( ( -LRB- cord-279980-49yv65gm 33 17 WHO who WP cord-279980-49yv65gm 33 18 , , , cord-279980-49yv65gm 33 19 2009 2009 CD cord-279980-49yv65gm 33 20 ) ) -RRB- cord-279980-49yv65gm 33 21 . . . cord-279980-49yv65gm 34 1 The the DT cord-279980-49yv65gm 34 2 influenza influenza NN cord-279980-49yv65gm 34 3 A a DT cord-279980-49yv65gm 34 4 virus virus NN cord-279980-49yv65gm 34 5 genome genome NN cord-279980-49yv65gm 34 6 comprises comprise VBZ cord-279980-49yv65gm 34 7 of of IN cord-279980-49yv65gm 35 1 eight eight CD cord-279980-49yv65gm 35 2 segmented segmented JJ cord-279980-49yv65gm 35 3 single single RB cord-279980-49yv65gm 35 4 - - HYPH cord-279980-49yv65gm 35 5 stranded strand VBN cord-279980-49yv65gm 35 6 negative negative JJ cord-279980-49yv65gm 35 7 sense sense NN cord-279980-49yv65gm 35 8 RNA RNA NNP cord-279980-49yv65gm 35 9 ( ( -LRB- cord-279980-49yv65gm 35 10 Engelhardt Engelhardt NNP cord-279980-49yv65gm 35 11 & & CC cord-279980-49yv65gm 35 12 Fodor Fodor NNP cord-279980-49yv65gm 35 13 , , , cord-279980-49yv65gm 35 14 2006 2006 CD cord-279980-49yv65gm 35 15 ) ) -RRB- cord-279980-49yv65gm 35 16 . . . cord-279980-49yv65gm 36 1 They -PRON- PRP cord-279980-49yv65gm 36 2 can can MD cord-279980-49yv65gm 36 3 be be VB cord-279980-49yv65gm 36 4 further far RBR cord-279980-49yv65gm 36 5 sub sub NN cord-279980-49yv65gm 36 6 - - HYPH cord-279980-49yv65gm 36 7 typed type VBN cord-279980-49yv65gm 36 8 based base VBN cord-279980-49yv65gm 36 9 on on IN cord-279980-49yv65gm 36 10 the the DT cord-279980-49yv65gm 36 11 two two CD cord-279980-49yv65gm 36 12 major major JJ cord-279980-49yv65gm 36 13 antigens antigen NNS cord-279980-49yv65gm 36 14 : : : cord-279980-49yv65gm 36 15 haemagglutinin haemagglutinin NNP cord-279980-49yv65gm 36 16 ( ( -LRB- cord-279980-49yv65gm 36 17 HA HA NNP cord-279980-49yv65gm 36 18 ) ) -RRB- cord-279980-49yv65gm 36 19 and and CC cord-279980-49yv65gm 36 20 neuraminidase neuraminidase NN cord-279980-49yv65gm 36 21 ( ( -LRB- cord-279980-49yv65gm 36 22 NA NA NNP cord-279980-49yv65gm 36 23 ) ) -RRB- cord-279980-49yv65gm 36 24 . . . cord-279980-49yv65gm 37 1 To to IN cord-279980-49yv65gm 37 2 date date NN cord-279980-49yv65gm 37 3 , , , cord-279980-49yv65gm 37 4 16 16 CD cord-279980-49yv65gm 37 5 subtypes subtype NNS cord-279980-49yv65gm 37 6 of of IN cord-279980-49yv65gm 37 7 HA HA NNP cord-279980-49yv65gm 37 8 ( ( -LRB- cord-279980-49yv65gm 37 9 H1 H1 NNP cord-279980-49yv65gm 37 10 - - HYPH cord-279980-49yv65gm 37 11 16 16 CD cord-279980-49yv65gm 37 12 ) ) -RRB- cord-279980-49yv65gm 37 13 and and CC cord-279980-49yv65gm 37 14 9 9 CD cord-279980-49yv65gm 37 15 of of IN cord-279980-49yv65gm 37 16 NA NA NNP cord-279980-49yv65gm 37 17 ( ( -LRB- cord-279980-49yv65gm 37 18 N1 N1 NNP cord-279980-49yv65gm 37 19 - - HYPH cord-279980-49yv65gm 37 20 9 9 CD cord-279980-49yv65gm 37 21 ) ) -RRB- cord-279980-49yv65gm 37 22 have have VBP cord-279980-49yv65gm 37 23 been be VBN cord-279980-49yv65gm 37 24 identified identify VBN cord-279980-49yv65gm 37 25 ( ( -LRB- cord-279980-49yv65gm 37 26 Fouchier Fouchier NNP cord-279980-49yv65gm 37 27 et et NNP cord-279980-49yv65gm 37 28 al al NNP cord-279980-49yv65gm 37 29 . . NNP cord-279980-49yv65gm 37 30 , , , cord-279980-49yv65gm 37 31 2005 2005 CD cord-279980-49yv65gm 37 32 ) ) -RRB- cord-279980-49yv65gm 37 33 . . . cord-279980-49yv65gm 38 1 In in IN cord-279980-49yv65gm 38 2 general general JJ cord-279980-49yv65gm 38 3 , , , cord-279980-49yv65gm 38 4 viruses virus NNS cord-279980-49yv65gm 38 5 of of IN cord-279980-49yv65gm 38 6 all all DT cord-279980-49yv65gm 38 7 HA HA NNP cord-279980-49yv65gm 38 8 and and CC cord-279980-49yv65gm 38 9 NA NA NNP cord-279980-49yv65gm 38 10 subtypes subtype NNS cord-279980-49yv65gm 38 11 are be VBP cord-279980-49yv65gm 38 12 found find VBN cord-279980-49yv65gm 38 13 in in IN cord-279980-49yv65gm 38 14 aquatic aquatic JJ cord-279980-49yv65gm 38 15 birds bird NNS cord-279980-49yv65gm 38 16 . . . cord-279980-49yv65gm 39 1 Among among IN cord-279980-49yv65gm 39 2 the the DT cord-279980-49yv65gm 39 3 16 16 CD cord-279980-49yv65gm 39 4 HA HA NNP cord-279980-49yv65gm 39 5 subtypes subtype NNS cord-279980-49yv65gm 39 6 , , , cord-279980-49yv65gm 39 7 only only RB cord-279980-49yv65gm 39 8 H5 H5 NNP cord-279980-49yv65gm 39 9 and and CC cord-279980-49yv65gm 39 10 H7 H7 NNP cord-279980-49yv65gm 39 11 are be VBP cord-279980-49yv65gm 39 12 virulent virulent JJ cord-279980-49yv65gm 39 13 in in IN cord-279980-49yv65gm 39 14 poultry poultry NN cord-279980-49yv65gm 39 15 ( ( -LRB- cord-279980-49yv65gm 39 16 Alexander Alexander NNP cord-279980-49yv65gm 39 17 , , , cord-279980-49yv65gm 39 18 2000 2000 CD cord-279980-49yv65gm 39 19 ) ) -RRB- cord-279980-49yv65gm 39 20 . . . cord-279980-49yv65gm 40 1 By by IN cord-279980-49yv65gm 40 2 contrast contrast NN cord-279980-49yv65gm 40 3 , , , cord-279980-49yv65gm 40 4 only only RB cord-279980-49yv65gm 40 5 three three CD cord-279980-49yv65gm 40 6 HA HA NNP cord-279980-49yv65gm 40 7 subtypes subtype NNS cord-279980-49yv65gm 40 8 ( ( -LRB- cord-279980-49yv65gm 40 9 H1 h1 NN cord-279980-49yv65gm 40 10 , , , cord-279980-49yv65gm 40 11 H2 H2 NNP cord-279980-49yv65gm 40 12 and and CC cord-279980-49yv65gm 40 13 H3 H3 NNP cord-279980-49yv65gm 40 14 ) ) -RRB- cord-279980-49yv65gm 40 15 and and CC cord-279980-49yv65gm 40 16 two two CD cord-279980-49yv65gm 40 17 NA NA NNP cord-279980-49yv65gm 40 18 subtypes subtype NNS cord-279980-49yv65gm 40 19 ( ( -LRB- cord-279980-49yv65gm 40 20 N1 N1 NNP cord-279980-49yv65gm 40 21 and and CC cord-279980-49yv65gm 40 22 N2 N2 NNP cord-279980-49yv65gm 40 23 ) ) -RRB- cord-279980-49yv65gm 40 24 have have VBP cord-279980-49yv65gm 40 25 commonly commonly RB cord-279980-49yv65gm 40 26 been be VBN cord-279980-49yv65gm 40 27 found find VBN cord-279980-49yv65gm 40 28 in in IN cord-279980-49yv65gm 40 29 humans human NNS cord-279980-49yv65gm 40 30 . . . cord-279980-49yv65gm 41 1 However however RB cord-279980-49yv65gm 41 2 , , , cord-279980-49yv65gm 41 3 over over IN cord-279980-49yv65gm 41 4 the the DT cord-279980-49yv65gm 41 5 past past JJ cord-279980-49yv65gm 41 6 few few JJ cord-279980-49yv65gm 41 7 years year NNS cord-279980-49yv65gm 41 8 , , , cord-279980-49yv65gm 41 9 several several JJ cord-279980-49yv65gm 41 10 subtypes subtype NNS cord-279980-49yv65gm 41 11 of of IN cord-279980-49yv65gm 41 12 AI AI NNP cord-279980-49yv65gm 41 13 viruses virus NNS cord-279980-49yv65gm 41 14 including include VBG cord-279980-49yv65gm 41 15 H5N1 H5N1 NNP cord-279980-49yv65gm 41 16 , , , cord-279980-49yv65gm 41 17 H7N7 H7N7 NNP cord-279980-49yv65gm 41 18 , , , cord-279980-49yv65gm 41 19 H7N3 H7N3 NNP cord-279980-49yv65gm 41 20 and and CC cord-279980-49yv65gm 41 21 H9N2 H9N2 NNP cord-279980-49yv65gm 41 22 have have VBP cord-279980-49yv65gm 41 23 directly directly RB cord-279980-49yv65gm 41 24 crossed cross VBN cord-279980-49yv65gm 41 25 the the DT cord-279980-49yv65gm 41 26 species specie NNS cord-279980-49yv65gm 41 27 barrier barrier NN cord-279980-49yv65gm 41 28 to to TO cord-279980-49yv65gm 41 29 infect infect VB cord-279980-49yv65gm 41 30 humans human NNS cord-279980-49yv65gm 41 31 ( ( -LRB- cord-279980-49yv65gm 41 32 Mahy Mahy NNP cord-279980-49yv65gm 41 33 & & CC cord-279980-49yv65gm 41 34 Van Van NNP cord-279980-49yv65gm 41 35 Regenmortel Regenmortel NNP cord-279980-49yv65gm 41 36 , , , cord-279980-49yv65gm 41 37 2008 2008 CD cord-279980-49yv65gm 41 38 ) ) -RRB- cord-279980-49yv65gm 41 39 . . . cord-279980-49yv65gm 42 1 House House NNP cord-279980-49yv65gm 42 2 flies fly VBZ cord-279980-49yv65gm 42 3 , , , cord-279980-49yv65gm 42 4 a a DT cord-279980-49yv65gm 42 5 synanthropic synanthropic JJ cord-279980-49yv65gm 42 6 species specie NNS cord-279980-49yv65gm 42 7 , , , cord-279980-49yv65gm 42 8 are be VBP cord-279980-49yv65gm 42 9 commonly commonly RB cord-279980-49yv65gm 42 10 found find VBN cord-279980-49yv65gm 42 11 associated associate VBN cord-279980-49yv65gm 42 12 with with IN cord-279980-49yv65gm 42 13 humans human NNS cord-279980-49yv65gm 42 14 and and CC cord-279980-49yv65gm 42 15 the the DT cord-279980-49yv65gm 42 16 animals animal NNS cord-279980-49yv65gm 42 17 they -PRON- PRP cord-279980-49yv65gm 42 18 raise raise VBP cord-279980-49yv65gm 43 1 ( ( -LRB- cord-279980-49yv65gm 43 2 Greenberg Greenberg NNP cord-279980-49yv65gm 43 3 , , , cord-279980-49yv65gm 43 4 1973 1973 CD cord-279980-49yv65gm 43 5 ) ) -RRB- cord-279980-49yv65gm 43 6 . . . cord-279980-49yv65gm 44 1 Importantly importantly RB cord-279980-49yv65gm 44 2 , , , cord-279980-49yv65gm 44 3 house house NN cord-279980-49yv65gm 44 4 flies fly NNS cord-279980-49yv65gm 44 5 can can MD cord-279980-49yv65gm 44 6 be be VB cord-279980-49yv65gm 44 7 mechanical mechanical JJ cord-279980-49yv65gm 44 8 and/or and/or CC cord-279980-49yv65gm 44 9 biological biological JJ cord-279980-49yv65gm 44 10 vectors vector NNS cord-279980-49yv65gm 44 11 for for IN cord-279980-49yv65gm 44 12 a a DT cord-279980-49yv65gm 44 13 number number NN cord-279980-49yv65gm 44 14 of of IN cord-279980-49yv65gm 44 15 major major JJ cord-279980-49yv65gm 44 16 pathogens pathogen NNS cord-279980-49yv65gm 44 17 in in IN cord-279980-49yv65gm 44 18 the the DT cord-279980-49yv65gm 44 19 animal animal NN cord-279980-49yv65gm 44 20 production production NN cord-279980-49yv65gm 44 21 system system NN cord-279980-49yv65gm 44 22 such such JJ cord-279980-49yv65gm 44 23 as as IN cord-279980-49yv65gm 44 24 Aeromonas Aeromonas NNP cord-279980-49yv65gm 44 25 caviae caviae NN cord-279980-49yv65gm 44 26 ( ( -LRB- cord-279980-49yv65gm 44 27 Nayduch Nayduch NNP cord-279980-49yv65gm 44 28 et et NNP cord-279980-49yv65gm 44 29 al al NNP cord-279980-49yv65gm 44 30 . . NNP cord-279980-49yv65gm 44 31 , , , cord-279980-49yv65gm 44 32 2002 2002 CD cord-279980-49yv65gm 44 33 ) ) -RRB- cord-279980-49yv65gm 44 34 , , , cord-279980-49yv65gm 44 35 Shigella Shigella NNP cord-279980-49yv65gm 44 36 spp spp NNS cord-279980-49yv65gm 44 37 . . . cord-279980-49yv65gm 45 1 ( ( -LRB- cord-279980-49yv65gm 45 2 Greenberg Greenberg NNP cord-279980-49yv65gm 45 3 , , , cord-279980-49yv65gm 45 4 1973 1973 CD cord-279980-49yv65gm 45 5 ) ) -RRB- cord-279980-49yv65gm 45 6 , , , cord-279980-49yv65gm 45 7 Salmonella Salmonella NNP cord-279980-49yv65gm 45 8 spp spp NNS cord-279980-49yv65gm 45 9 . . . cord-279980-49yv65gm 46 1 ( ( -LRB- cord-279980-49yv65gm 46 2 Holt Holt NNP cord-279980-49yv65gm 46 3 et et NNP cord-279980-49yv65gm 46 4 al al NNP cord-279980-49yv65gm 46 5 . . NNP cord-279980-49yv65gm 46 6 , , , cord-279980-49yv65gm 46 7 2007 2007 CD cord-279980-49yv65gm 46 8 ) ) -RRB- cord-279980-49yv65gm 46 9 , , , cord-279980-49yv65gm 46 10 Escherichia Escherichia NNP cord-279980-49yv65gm 46 11 coli coli NNS cord-279980-49yv65gm 46 12 ( ( -LRB- cord-279980-49yv65gm 46 13 De De NNP cord-279980-49yv65gm 46 14 Jesus Jesus NNP cord-279980-49yv65gm 46 15 et et NNP cord-279980-49yv65gm 46 16 al al NNP cord-279980-49yv65gm 46 17 . . NNP cord-279980-49yv65gm 46 18 , , , cord-279980-49yv65gm 46 19 2004 2004 CD cord-279980-49yv65gm 46 20 ) ) -RRB- cord-279980-49yv65gm 46 21 , , , cord-279980-49yv65gm 46 22 Vibrio Vibrio NNP cord-279980-49yv65gm 46 23 cholerae cholerae NN cord-279980-49yv65gm 46 24 ( ( -LRB- cord-279980-49yv65gm 46 25 Yap Yap NNP cord-279980-49yv65gm 46 26 et et NNP cord-279980-49yv65gm 46 27 al al NNP cord-279980-49yv65gm 46 28 . . NNP cord-279980-49yv65gm 46 29 , , , cord-279980-49yv65gm 46 30 2008 2008 CD cord-279980-49yv65gm 46 31 ) ) -RRB- cord-279980-49yv65gm 46 32 , , , cord-279980-49yv65gm 46 33 Newcastle Newcastle NNP cord-279980-49yv65gm 46 34 disease disease NN cord-279980-49yv65gm 46 35 virus virus NN cord-279980-49yv65gm 46 36 ( ( -LRB- cord-279980-49yv65gm 46 37 NDV NDV NNP cord-279980-49yv65gm 46 38 ) ) -RRB- cord-279980-49yv65gm 47 1 ( ( -LRB- cord-279980-49yv65gm 47 2 Watson Watson NNP cord-279980-49yv65gm 47 3 et et NNP cord-279980-49yv65gm 47 4 al al NNP cord-279980-49yv65gm 47 5 . . NNP cord-279980-49yv65gm 47 6 , , , cord-279980-49yv65gm 47 7 2007 2007 CD cord-279980-49yv65gm 47 8 ) ) -RRB- cord-279980-49yv65gm 47 9 , , , cord-279980-49yv65gm 47 10 turkey turkey NN cord-279980-49yv65gm 47 11 coronavirus coronavirus NN cord-279980-49yv65gm 47 12 ( ( -LRB- cord-279980-49yv65gm 47 13 Calibeo Calibeo NNP cord-279980-49yv65gm 47 14 - - HYPH cord-279980-49yv65gm 47 15 Hayes Hayes NNP cord-279980-49yv65gm 47 16 et et NNP cord-279980-49yv65gm 47 17 al al NNP cord-279980-49yv65gm 47 18 . . NNP cord-279980-49yv65gm 47 19 , , , cord-279980-49yv65gm 47 20 2003 2003 CD cord-279980-49yv65gm 47 21 ) ) -RRB- cord-279980-49yv65gm 47 22 , , , cord-279980-49yv65gm 47 23 porcine porcine JJ cord-279980-49yv65gm 47 24 reproductive reproductive JJ cord-279980-49yv65gm 47 25 and and CC cord-279980-49yv65gm 47 26 respiratory respiratory JJ cord-279980-49yv65gm 47 27 syndrome syndrome NN cord-279980-49yv65gm 47 28 virus virus NN cord-279980-49yv65gm 48 1 ( ( -LRB- cord-279980-49yv65gm 48 2 Otake Otake NNP cord-279980-49yv65gm 48 3 et et NNP cord-279980-49yv65gm 48 4 al al NNP cord-279980-49yv65gm 48 5 . . NNP cord-279980-49yv65gm 48 6 , , , cord-279980-49yv65gm 48 7 2003 2003 CD cord-279980-49yv65gm 48 8 ) ) -RRB- cord-279980-49yv65gm 48 9 , , , cord-279980-49yv65gm 48 10 rotavirus rotavirus NN cord-279980-49yv65gm 48 11 ( ( -LRB- cord-279980-49yv65gm 48 12 Tan Tan NNP cord-279980-49yv65gm 48 13 et et FW cord-279980-49yv65gm 48 14 al al NNP cord-279980-49yv65gm 48 15 . . NNP cord-279980-49yv65gm 48 16 , , , cord-279980-49yv65gm 48 17 1997 1997 CD cord-279980-49yv65gm 48 18 ) ) -RRB- cord-279980-49yv65gm 48 19 , , , cord-279980-49yv65gm 48 20 Microsporum Microsporum NNP cord-279980-49yv65gm 48 21 canis canis NN cord-279980-49yv65gm 48 22 ( ( -LRB- cord-279980-49yv65gm 48 23 Cafarchia Cafarchia NNP cord-279980-49yv65gm 48 24 et et NNP cord-279980-49yv65gm 48 25 al al NNP cord-279980-49yv65gm 48 26 . . NNP cord-279980-49yv65gm 48 27 , , , cord-279980-49yv65gm 48 28 2009 2009 CD cord-279980-49yv65gm 48 29 ) ) -RRB- cord-279980-49yv65gm 48 30 and and CC cord-279980-49yv65gm 48 31 metazoan metazoan NN cord-279980-49yv65gm 48 32 parasites parasite NNS cord-279980-49yv65gm 48 33 ( ( -LRB- cord-279980-49yv65gm 48 34 Förster Förster NNP cord-279980-49yv65gm 48 35 et et NNP cord-279980-49yv65gm 48 36 al al NNP cord-279980-49yv65gm 48 37 . . NNP cord-279980-49yv65gm 48 38 , , , cord-279980-49yv65gm 48 39 2007 2007 CD cord-279980-49yv65gm 48 40 ) ) -RRB- cord-279980-49yv65gm 48 41 . . . cord-279980-49yv65gm 49 1 House house NN cord-279980-49yv65gm 49 2 flies fly VBZ cord-279980-49yv65gm 49 3 transmit transmit VBP cord-279980-49yv65gm 49 4 pathogens pathogen NNS cord-279980-49yv65gm 49 5 via via IN cord-279980-49yv65gm 49 6 mouthparts mouthpart NNS cord-279980-49yv65gm 49 7 , , , cord-279980-49yv65gm 49 8 vomit vomit NN cord-279980-49yv65gm 49 9 droplets droplet NNS cord-279980-49yv65gm 49 10 , , , cord-279980-49yv65gm 49 11 faeces faece NNS cord-279980-49yv65gm 49 12 and and CC cord-279980-49yv65gm 49 13 their -PRON- PRP$ cord-279980-49yv65gm 49 14 body body NN cord-279980-49yv65gm 49 15 surface surface NN cord-279980-49yv65gm 49 16 ( ( -LRB- cord-279980-49yv65gm 49 17 Greenberg Greenberg NNP cord-279980-49yv65gm 49 18 , , , cord-279980-49yv65gm 49 19 1973 1973 CD cord-279980-49yv65gm 49 20 ) ) -RRB- cord-279980-49yv65gm 49 21 . . . cord-279980-49yv65gm 50 1 Their -PRON- PRP$ cord-279980-49yv65gm 50 2 ability ability NN cord-279980-49yv65gm 50 3 to to TO cord-279980-49yv65gm 50 4 disperse disperse VB cord-279980-49yv65gm 50 5 contaminated contaminated JJ cord-279980-49yv65gm 50 6 pathogens pathogen NNS cord-279980-49yv65gm 50 7 correlates correlate VBZ cord-279980-49yv65gm 50 8 with with IN cord-279980-49yv65gm 50 9 the the DT cord-279980-49yv65gm 50 10 flying fly VBG cord-279980-49yv65gm 50 11 distance distance NN cord-279980-49yv65gm 50 12 which which WDT cord-279980-49yv65gm 50 13 was be VBD cord-279980-49yv65gm 50 14 shown show VBN cord-279980-49yv65gm 50 15 to to TO cord-279980-49yv65gm 50 16 be be VB cord-279980-49yv65gm 50 17 approximately approximately RB cord-279980-49yv65gm 50 18 1 1 CD cord-279980-49yv65gm 50 19 - - SYM cord-279980-49yv65gm 50 20 3 3 CD cord-279980-49yv65gm 50 21 km km NN cord-279980-49yv65gm 50 22 per per IN cord-279980-49yv65gm 50 23 day day NN cord-279980-49yv65gm 50 24 ( ( -LRB- cord-279980-49yv65gm 50 25 Herms Herms NNP cord-279980-49yv65gm 50 26 et et NNP cord-279980-49yv65gm 50 27 al al NNP cord-279980-49yv65gm 50 28 . . NNP cord-279980-49yv65gm 50 29 , , , cord-279980-49yv65gm 50 30 1969 1969 CD cord-279980-49yv65gm 50 31 ) ) -RRB- cord-279980-49yv65gm 50 32 . . . cord-279980-49yv65gm 51 1 Accordingly accordingly RB cord-279980-49yv65gm 51 2 , , , cord-279980-49yv65gm 51 3 house house NN cord-279980-49yv65gm 51 4 flies fly NNS cord-279980-49yv65gm 51 5 are be VBP cord-279980-49yv65gm 51 6 considered consider VBN cord-279980-49yv65gm 51 7 as as IN cord-279980-49yv65gm 51 8 an an DT cord-279980-49yv65gm 51 9 important important JJ cord-279980-49yv65gm 51 10 vector vector NN cord-279980-49yv65gm 51 11 for for IN cord-279980-49yv65gm 51 12 spreading spread VBG cord-279980-49yv65gm 51 13 disease disease NN cord-279980-49yv65gm 51 14 in in IN cord-279980-49yv65gm 51 15 the the DT cord-279980-49yv65gm 51 16 poultry poultry NN cord-279980-49yv65gm 51 17 production production NN cord-279980-49yv65gm 51 18 system system NN cord-279980-49yv65gm 51 19 . . . cord-279980-49yv65gm 52 1 The the DT cord-279980-49yv65gm 52 2 objective objective NN cord-279980-49yv65gm 52 3 of of IN cord-279980-49yv65gm 52 4 the the DT cord-279980-49yv65gm 52 5 present present JJ cord-279980-49yv65gm 52 6 study study NN cord-279980-49yv65gm 52 7 was be VBD cord-279980-49yv65gm 52 8 to to TO cord-279980-49yv65gm 52 9 determine determine VB cord-279980-49yv65gm 52 10 the the DT cord-279980-49yv65gm 52 11 potential potential NN cord-279980-49yv65gm 52 12 of of IN cord-279980-49yv65gm 52 13 the the DT cord-279980-49yv65gm 52 14 house house NN cord-279980-49yv65gm 52 15 fly fly VB cord-279980-49yv65gm 52 16 as as IN cord-279980-49yv65gm 52 17 a a DT cord-279980-49yv65gm 52 18 vector vector NN cord-279980-49yv65gm 52 19 of of IN cord-279980-49yv65gm 52 20 the the DT cord-279980-49yv65gm 52 21 AI AI NNP cord-279980-49yv65gm 52 22 H5N1 H5N1 NNP cord-279980-49yv65gm 52 23 virus virus NN cord-279980-49yv65gm 52 24 . . . cord-279980-49yv65gm 53 1 The the DT cord-279980-49yv65gm 53 2 highly highly RB cord-279980-49yv65gm 53 3 pathogenic pathogenic JJ cord-279980-49yv65gm 53 4 avian avian JJ cord-279980-49yv65gm 53 5 influenza influenza NN cord-279980-49yv65gm 53 6 ( ( -LRB- cord-279980-49yv65gm 53 7 HPAI HPAI NNP cord-279980-49yv65gm 53 8 ) ) -RRB- cord-279980-49yv65gm 54 1 A a LS cord-279980-49yv65gm 54 2 / / SYM cord-279980-49yv65gm 54 3 Chicken/ Chicken/ NNP cord-279980-49yv65gm 54 4 Nakorn Nakorn NNP cord-279980-49yv65gm 54 5 - - HYPH cord-279980-49yv65gm 54 6 Pathom Pathom NNP cord-279980-49yv65gm 54 7 / / SYM cord-279980-49yv65gm 54 8 Thailand Thailand NNP cord-279980-49yv65gm 54 9 / / SYM cord-279980-49yv65gm 54 10 CU CU NNP cord-279980-49yv65gm 54 11 - - HYPH cord-279980-49yv65gm 54 12 K2/04 K2/04 NNP cord-279980-49yv65gm 54 13 ( ( -LRB- cord-279980-49yv65gm 54 14 H5N1 H5N1 NNP cord-279980-49yv65gm 54 15 ) ) -RRB- cord-279980-49yv65gm 54 16 virus virus NN cord-279980-49yv65gm 54 17 used use VBN cord-279980-49yv65gm 54 18 in in IN cord-279980-49yv65gm 54 19 the the DT cord-279980-49yv65gm 54 20 present present JJ cord-279980-49yv65gm 54 21 study study NN cord-279980-49yv65gm 54 22 was be VBD cord-279980-49yv65gm 54 23 propagated propagate VBN cord-279980-49yv65gm 54 24 in in IN cord-279980-49yv65gm 54 25 the the DT cord-279980-49yv65gm 54 26 allantoic allantoic NNP cord-279980-49yv65gm 54 27 cavity cavity NN cord-279980-49yv65gm 54 28 of of IN cord-279980-49yv65gm 54 29 10-dayold 10-dayold CD cord-279980-49yv65gm 54 30 embryonated embryonate VBN cord-279980-49yv65gm 54 31 chicken chicken NN cord-279980-49yv65gm 54 32 eggs egg NNS cord-279980-49yv65gm 54 33 using use VBG cord-279980-49yv65gm 54 34 standard standard JJ cord-279980-49yv65gm 54 35 protocol protocol NN cord-279980-49yv65gm 54 36 ( ( -LRB- cord-279980-49yv65gm 54 37 OIE OIE NNP cord-279980-49yv65gm 54 38 , , , cord-279980-49yv65gm 54 39 2000 2000 CD cord-279980-49yv65gm 54 40 ) ) -RRB- cord-279980-49yv65gm 54 41 . . . cord-279980-49yv65gm 55 1 Allantoic Allantoic NNP cord-279980-49yv65gm 55 2 fluid fluid NN cord-279980-49yv65gm 55 3 of of IN cord-279980-49yv65gm 55 4 the the DT cord-279980-49yv65gm 55 5 third third JJ cord-279980-49yv65gm 55 6 passage passage NN cord-279980-49yv65gm 55 7 of of IN cord-279980-49yv65gm 55 8 viral viral JJ cord-279980-49yv65gm 55 9 inoculated inoculate VBN cord-279980-49yv65gm 55 10 embryonated embryonate VBN cord-279980-49yv65gm 55 11 chicken chicken NN cord-279980-49yv65gm 55 12 eggs egg NNS cord-279980-49yv65gm 55 13 was be VBD cord-279980-49yv65gm 55 14 used use VBN cord-279980-49yv65gm 55 15 in in IN cord-279980-49yv65gm 55 16 this this DT cord-279980-49yv65gm 55 17 experiment experiment NN cord-279980-49yv65gm 55 18 . . . cord-279980-49yv65gm 56 1 Virus virus NN cord-279980-49yv65gm 56 2 titre titre NN cord-279980-49yv65gm 56 3 was be VBD cord-279980-49yv65gm 56 4 determined determine VBN cord-279980-49yv65gm 56 5 10 10 CD cord-279980-49yv65gm 56 6 9.5 9.5 CD cord-279980-49yv65gm 56 7 50 50 CD cord-279980-49yv65gm 56 8 % % NN cord-279980-49yv65gm 56 9 of of IN cord-279980-49yv65gm 56 10 embryonated embryonated JJ cord-279980-49yv65gm 56 11 lethal lethal JJ cord-279980-49yv65gm 56 12 dose dose NN cord-279980-49yv65gm 56 13 / / SYM cord-279980-49yv65gm 56 14 millilitre millilitre NN cord-279980-49yv65gm 56 15 ( ( -LRB- cord-279980-49yv65gm 56 16 ELD ELD NNP cord-279980-49yv65gm 56 17 50 50 CD cord-279980-49yv65gm 56 18 /mL /mL . cord-279980-49yv65gm 56 19 ) ) -RRB- cord-279980-49yv65gm 56 20 and and CC cord-279980-49yv65gm 56 21 the the DT cord-279980-49yv65gm 56 22 virus virus NN cord-279980-49yv65gm 56 23 was be VBD cord-279980-49yv65gm 56 24 stored store VBN cord-279980-49yv65gm 56 25 at at IN cord-279980-49yv65gm 57 1 −80 −80 NNP cord-279980-49yv65gm 57 2 • • NNP cord-279980-49yv65gm 57 3 C C NNP cord-279980-49yv65gm 57 4 until until IN cord-279980-49yv65gm 57 5 used use VBN cord-279980-49yv65gm 57 6 . . . cord-279980-49yv65gm 58 1 House house NN cord-279980-49yv65gm 58 2 flies fly NNS cord-279980-49yv65gm 58 3 were be VBD cord-279980-49yv65gm 58 4 obtained obtain VBN cord-279980-49yv65gm 58 5 as as IN cord-279980-49yv65gm 58 6 larvae larva NNS cord-279980-49yv65gm 58 7 from from IN cord-279980-49yv65gm 58 8 a a DT cord-279980-49yv65gm 58 9 layer layer NN cord-279980-49yv65gm 58 10 chicken chicken NN cord-279980-49yv65gm 58 11 farm farm NN cord-279980-49yv65gm 58 12 in in IN cord-279980-49yv65gm 58 13 Thailand Thailand NNP cord-279980-49yv65gm 58 14 ( ( -LRB- cord-279980-49yv65gm 58 15 5.4 5.4 CD cord-279980-49yv65gm 58 16 - - SYM cord-279980-49yv65gm 58 17 20.6 20.6 CD cord-279980-49yv65gm 58 18 • • NN cord-279980-49yv65gm 58 19 N n CD cord-279980-49yv65gm 58 20 and and CC cord-279980-49yv65gm 58 21 97.1 97.1 CD cord-279980-49yv65gm 58 22 - - SYM cord-279980-49yv65gm 58 23 106.0 106.0 CD cord-279980-49yv65gm 58 24 • • NN cord-279980-49yv65gm 58 25 E e NN cord-279980-49yv65gm 58 26 ) ) -RRB- cord-279980-49yv65gm 58 27 . . . cord-279980-49yv65gm 59 1 The the DT cord-279980-49yv65gm 59 2 fly fly NN cord-279980-49yv65gm 59 3 larvae larva NNS cord-279980-49yv65gm 59 4 were be VBD cord-279980-49yv65gm 59 5 kept keep VBN cord-279980-49yv65gm 59 6 in in IN cord-279980-49yv65gm 59 7 a a DT cord-279980-49yv65gm 59 8 sand sand NN cord-279980-49yv65gm 59 9 box box NN cord-279980-49yv65gm 59 10 containing contain VBG cord-279980-49yv65gm 59 11 larval larval JJ cord-279980-49yv65gm 59 12 medium medium NN cord-279980-49yv65gm 59 13 which which WDT cord-279980-49yv65gm 59 14 comprised comprise VBN cord-279980-49yv65gm 59 15 of of IN cord-279980-49yv65gm 59 16 swine swine NN cord-279980-49yv65gm 59 17 livers liver NNS cord-279980-49yv65gm 59 18 at at IN cord-279980-49yv65gm 59 19 room room NN cord-279980-49yv65gm 59 20 temperature temperature NN cord-279980-49yv65gm 59 21 until until IN cord-279980-49yv65gm 59 22 pupation pupation NN cord-279980-49yv65gm 59 23 . . . cord-279980-49yv65gm 60 1 The the DT cord-279980-49yv65gm 60 2 pupae pupa NNS cord-279980-49yv65gm 60 3 were be VBD cord-279980-49yv65gm 60 4 harvested harvest VBN cord-279980-49yv65gm 60 5 and and CC cord-279980-49yv65gm 60 6 stored store VBN cord-279980-49yv65gm 60 7 in in IN cord-279980-49yv65gm 60 8 a a DT cord-279980-49yv65gm 60 9 plastic plastic JJ cord-279980-49yv65gm 60 10 box box NN cord-279980-49yv65gm 60 11 at at IN cord-279980-49yv65gm 60 12 room room NN cord-279980-49yv65gm 60 13 temperature temperature NN cord-279980-49yv65gm 60 14 until until IN cord-279980-49yv65gm 60 15 the the DT cord-279980-49yv65gm 60 16 emergence emergence NN cord-279980-49yv65gm 60 17 of of IN cord-279980-49yv65gm 60 18 adults adult NNS cord-279980-49yv65gm 60 19 . . . cord-279980-49yv65gm 61 1 The the DT cord-279980-49yv65gm 61 2 emergence emergence NN cord-279980-49yv65gm 61 3 of of IN cord-279980-49yv65gm 61 4 adult adult NN cord-279980-49yv65gm 61 5 flies fly NNS cord-279980-49yv65gm 61 6 represent represent VBP cord-279980-49yv65gm 61 7 day day NN cord-279980-49yv65gm 61 8 0 0 CD cord-279980-49yv65gm 61 9 . . . cord-279980-49yv65gm 62 1 Before before IN cord-279980-49yv65gm 62 2 initiation initiation NN cord-279980-49yv65gm 62 3 of of IN cord-279980-49yv65gm 62 4 all all DT cord-279980-49yv65gm 62 5 experiments experiment NNS cord-279980-49yv65gm 62 6 the the DT cord-279980-49yv65gm 62 7 adult adult NN cord-279980-49yv65gm 62 8 stage stage NN cord-279980-49yv65gm 62 9 flies fly NNS cord-279980-49yv65gm 62 10 were be VBD cord-279980-49yv65gm 62 11 randomly randomly RB cord-279980-49yv65gm 62 12 selected select VBN cord-279980-49yv65gm 62 13 and and CC cord-279980-49yv65gm 62 14 confirmed confirm VBD cord-279980-49yv65gm 62 15 to to TO cord-279980-49yv65gm 62 16 be be VB cord-279980-49yv65gm 62 17 free free JJ cord-279980-49yv65gm 62 18 of of IN cord-279980-49yv65gm 62 19 the the DT cord-279980-49yv65gm 62 20 AI AI NNP cord-279980-49yv65gm 62 21 H5N1 H5N1 NNP cord-279980-49yv65gm 62 22 virus virus NN cord-279980-49yv65gm 62 23 by by IN cord-279980-49yv65gm 62 24 the the DT cord-279980-49yv65gm 62 25 real real JJ cord-279980-49yv65gm 62 26 - - HYPH cord-279980-49yv65gm 62 27 time time NN cord-279980-49yv65gm 62 28 reverse reverse NN cord-279980-49yv65gm 62 29 transcription transcription NN cord-279980-49yv65gm 62 30 - - HYPH cord-279980-49yv65gm 62 31 polymerase polymerase NN cord-279980-49yv65gm 62 32 chain chain NN cord-279980-49yv65gm 62 33 reaction reaction NN cord-279980-49yv65gm 62 34 ( ( -LRB- cord-279980-49yv65gm 62 35 RRT RRT NNP cord-279980-49yv65gm 62 36 - - HYPH cord-279980-49yv65gm 62 37 PCR PCR NNP cord-279980-49yv65gm 62 38 ) ) -RRB- cord-279980-49yv65gm 62 39 assay assay NN cord-279980-49yv65gm 62 40 using use VBG cord-279980-49yv65gm 62 41 specific specific JJ cord-279980-49yv65gm 62 42 primers primer NNS cord-279980-49yv65gm 62 43 and and CC cord-279980-49yv65gm 62 44 probes probe NNS cord-279980-49yv65gm 62 45 to to IN cord-279980-49yv65gm 62 46 the the DT cord-279980-49yv65gm 62 47 influenza influenza NN cord-279980-49yv65gm 62 48 A a DT cord-279980-49yv65gm 62 49 matrix matrix NN cord-279980-49yv65gm 62 50 ( ( -LRB- cord-279980-49yv65gm 62 51 M M NNP cord-279980-49yv65gm 62 52 ) ) -RRB- cord-279980-49yv65gm 62 53 gene gene NN cord-279980-49yv65gm 62 54 . . . cord-279980-49yv65gm 63 1 The the DT cord-279980-49yv65gm 63 2 2-day 2-day JJ cord-279980-49yv65gm 63 3 - - HYPH cord-279980-49yv65gm 63 4 old old JJ cord-279980-49yv65gm 63 5 house house NN cord-279980-49yv65gm 63 6 flies fly NNS cord-279980-49yv65gm 63 7 were be VBD cord-279980-49yv65gm 63 8 separated separate VBN cord-279980-49yv65gm 63 9 for for IN cord-279980-49yv65gm 63 10 use use NN cord-279980-49yv65gm 63 11 in in IN cord-279980-49yv65gm 63 12 the the DT cord-279980-49yv65gm 63 13 following follow VBG cord-279980-49yv65gm 63 14 experiments experiment NNS cord-279980-49yv65gm 63 15 . . . cord-279980-49yv65gm 64 1 Three three CD cord-279980-49yv65gm 64 2 experiments experiment NNS cord-279980-49yv65gm 64 3 were be VBD cord-279980-49yv65gm 64 4 conducted conduct VBN cord-279980-49yv65gm 64 5 to to TO cord-279980-49yv65gm 64 6 determine determine VB cord-279980-49yv65gm 64 7 the the DT cord-279980-49yv65gm 64 8 potential potential NN cord-279980-49yv65gm 64 9 of of IN cord-279980-49yv65gm 64 10 house house NN cord-279980-49yv65gm 64 11 flies fly VBZ cord-279980-49yv65gm 64 12 to to TO cord-279980-49yv65gm 64 13 harbour harbour VB cord-279980-49yv65gm 64 14 the the DT cord-279980-49yv65gm 64 15 AI AI NNP cord-279980-49yv65gm 64 16 H5N1 H5N1 NNP cord-279980-49yv65gm 64 17 virus virus NN cord-279980-49yv65gm 64 18 . . . cord-279980-49yv65gm 65 1 In in IN cord-279980-49yv65gm 65 2 all all DT cord-279980-49yv65gm 65 3 experiments experiment NNS cord-279980-49yv65gm 65 4 , , , cord-279980-49yv65gm 65 5 2-day 2-day JJ cord-279980-49yv65gm 65 6 - - HYPH cord-279980-49yv65gm 65 7 old old JJ cord-279980-49yv65gm 65 8 flies fly NNS cord-279980-49yv65gm 65 9 of of IN cord-279980-49yv65gm 65 10 mixed mixed JJ cord-279980-49yv65gm 65 11 sex sex NN cord-279980-49yv65gm 65 12 were be VBD cord-279980-49yv65gm 65 13 starved starve VBN cord-279980-49yv65gm 65 14 for for IN cord-279980-49yv65gm 65 15 12 12 CD cord-279980-49yv65gm 65 16 h h NN cord-279980-49yv65gm 65 17 before before IN cord-279980-49yv65gm 65 18 use use NN cord-279980-49yv65gm 65 19 . . . cord-279980-49yv65gm 66 1 Experiment experiment NN cord-279980-49yv65gm 66 2 1 1 CD cord-279980-49yv65gm 66 3 . . . cord-279980-49yv65gm 67 1 To to TO cord-279980-49yv65gm 67 2 evaluate evaluate VB cord-279980-49yv65gm 67 3 the the DT cord-279980-49yv65gm 67 4 potential potential NN cord-279980-49yv65gm 67 5 of of IN cord-279980-49yv65gm 67 6 house house NN cord-279980-49yv65gm 67 7 flies fly VBZ cord-279980-49yv65gm 67 8 to to TO cord-279980-49yv65gm 67 9 harbour harbour VB cord-279980-49yv65gm 67 10 the the DT cord-279980-49yv65gm 67 11 AI AI NNP cord-279980-49yv65gm 67 12 H5N1 H5N1 NNP cord-279980-49yv65gm 67 13 virus virus NN cord-279980-49yv65gm 67 14 , , , cord-279980-49yv65gm 67 15 100 100 CD cord-279980-49yv65gm 67 16 house house NN cord-279980-49yv65gm 67 17 flies fly NNS cord-279980-49yv65gm 67 18 were be VBD cord-279980-49yv65gm 67 19 separated separate VBN cord-279980-49yv65gm 67 20 into into IN cord-279980-49yv65gm 67 21 two two CD cord-279980-49yv65gm 67 22 groups group NNS cord-279980-49yv65gm 67 23 ( ( -LRB- cord-279980-49yv65gm 67 24 n n NN cord-279980-49yv65gm 67 25 = = SYM cord-279980-49yv65gm 67 26 50 50 CD cord-279980-49yv65gm 67 27 ) ) -RRB- cord-279980-49yv65gm 67 28 ; ; : cord-279980-49yv65gm 67 29 control control NN cord-279980-49yv65gm 67 30 and and CC cord-279980-49yv65gm 67 31 treatment treatment NN cord-279980-49yv65gm 67 32 . . . cord-279980-49yv65gm 68 1 The the DT cord-279980-49yv65gm 68 2 treatment treatment NN cord-279980-49yv65gm 68 3 group group NN cord-279980-49yv65gm 68 4 was be VBD cord-279980-49yv65gm 68 5 fed feed VBN cord-279980-49yv65gm 68 6 with with IN cord-279980-49yv65gm 68 7 a a DT cord-279980-49yv65gm 68 8 virus virus NN cord-279980-49yv65gm 68 9 mixture mixture NN cord-279980-49yv65gm 68 10 ( ( -LRB- cord-279980-49yv65gm 68 11 1 1 CD cord-279980-49yv65gm 68 12 mL ml NN cord-279980-49yv65gm 68 13 of of IN cord-279980-49yv65gm 68 14 allantoic allantoic NNP cord-279980-49yv65gm 68 15 fluid fluid NN cord-279980-49yv65gm 68 16 containing contain VBG cord-279980-49yv65gm 68 17 the the DT cord-279980-49yv65gm 68 18 AI AI NNP cord-279980-49yv65gm 68 19 H5N1 H5N1 NNP cord-279980-49yv65gm 68 20 virus virus NN cord-279980-49yv65gm 68 21 with with IN cord-279980-49yv65gm 68 22 1 1 CD cord-279980-49yv65gm 68 23 mL ml NN cord-279980-49yv65gm 68 24 of of IN cord-279980-49yv65gm 68 25 UHT UHT NNP cord-279980-49yv65gm 68 26 ( ( -LRB- cord-279980-49yv65gm 68 27 ultra ultra JJ cord-279980-49yv65gm 68 28 high high JJ cord-279980-49yv65gm 68 29 temperature temperature NN cord-279980-49yv65gm 68 30 ) ) -RRB- cord-279980-49yv65gm 68 31 processed process VBN cord-279980-49yv65gm 68 32 milk milk NN cord-279980-49yv65gm 68 33 mixed mix VBN cord-279980-49yv65gm 68 34 with with IN cord-279980-49yv65gm 68 35 10 10 CD cord-279980-49yv65gm 68 36 % % NN cord-279980-49yv65gm 68 37 sucrose sucrose NN cord-279980-49yv65gm 68 38 solution solution NN cord-279980-49yv65gm 68 39 ) ) -RRB- cord-279980-49yv65gm 68 40 . . . cord-279980-49yv65gm 69 1 The the DT cord-279980-49yv65gm 69 2 final final JJ cord-279980-49yv65gm 69 3 virus virus NN cord-279980-49yv65gm 69 4 titre titre NN cord-279980-49yv65gm 69 5 in in IN cord-279980-49yv65gm 69 6 the the DT cord-279980-49yv65gm 69 7 mixture mixture NN cord-279980-49yv65gm 69 8 was be VBD cord-279980-49yv65gm 69 9 1 1 CD cord-279980-49yv65gm 69 10 × × CD cord-279980-49yv65gm 69 11 10 10 CD cord-279980-49yv65gm 69 12 9.2 9.2 CD cord-279980-49yv65gm 69 13 ELD ELD NNP cord-279980-49yv65gm 69 14 50 50 CD cord-279980-49yv65gm 69 15 /mL. /mL. . cord-279980-49yv65gm 70 1 The the DT cord-279980-49yv65gm 70 2 mixture mixture NN cord-279980-49yv65gm 70 3 was be VBD cord-279980-49yv65gm 70 4 placed place VBN cord-279980-49yv65gm 70 5 on on IN cord-279980-49yv65gm 70 6 a a DT cord-279980-49yv65gm 70 7 cotton cotton NN cord-279980-49yv65gm 70 8 pad pad NN cord-279980-49yv65gm 70 9 on on IN cord-279980-49yv65gm 70 10 top top NN cord-279980-49yv65gm 70 11 of of IN cord-279980-49yv65gm 70 12 the the DT cord-279980-49yv65gm 70 13 box box NN cord-279980-49yv65gm 70 14 of of IN cord-279980-49yv65gm 70 15 the the DT cord-279980-49yv65gm 70 16 treatment treatment NN cord-279980-49yv65gm 70 17 group group NN cord-279980-49yv65gm 70 18 . . . cord-279980-49yv65gm 71 1 The the DT cord-279980-49yv65gm 71 2 control control NN cord-279980-49yv65gm 71 3 group group NN cord-279980-49yv65gm 71 4 was be VBD cord-279980-49yv65gm 71 5 fed feed VBN cord-279980-49yv65gm 71 6 with with IN cord-279980-49yv65gm 71 7 the the DT cord-279980-49yv65gm 71 8 same same JJ cord-279980-49yv65gm 71 9 amount amount NN cord-279980-49yv65gm 71 10 of of IN cord-279980-49yv65gm 71 11 UHT UHT NNP cord-279980-49yv65gm 71 12 milk milk NN cord-279980-49yv65gm 71 13 mixed mix VBN cord-279980-49yv65gm 71 14 with with IN cord-279980-49yv65gm 71 15 10 10 CD cord-279980-49yv65gm 71 16 % % NN cord-279980-49yv65gm 71 17 sucrose sucrose NN cord-279980-49yv65gm 71 18 solution solution NN cord-279980-49yv65gm 71 19 without without IN cord-279980-49yv65gm 71 20 the the DT cord-279980-49yv65gm 71 21 virus virus NN cord-279980-49yv65gm 71 22 . . . cord-279980-49yv65gm 72 1 House house NN cord-279980-49yv65gm 72 2 flies fly NNS cord-279980-49yv65gm 72 3 were be VBD cord-279980-49yv65gm 72 4 allowed allow VBN cord-279980-49yv65gm 72 5 15 15 CD cord-279980-49yv65gm 72 6 min min NN cord-279980-49yv65gm 72 7 of of IN cord-279980-49yv65gm 72 8 feeding feed VBG cord-279980-49yv65gm 72 9 time time NN cord-279980-49yv65gm 72 10 and and CC cord-279980-49yv65gm 72 11 all all DT cord-279980-49yv65gm 72 12 flies fly NNS cord-279980-49yv65gm 72 13 were be VBD cord-279980-49yv65gm 72 14 then then RB cord-279980-49yv65gm 72 15 euthanized euthanize VBN cord-279980-49yv65gm 72 16 with with IN cord-279980-49yv65gm 72 17 dry dry JJ cord-279980-49yv65gm 72 18 ice ice NN cord-279980-49yv65gm 72 19 for for IN cord-279980-49yv65gm 72 20 15 15 CD cord-279980-49yv65gm 72 21 min min NN cord-279980-49yv65gm 72 22 . . . cord-279980-49yv65gm 73 1 Each each DT cord-279980-49yv65gm 73 2 group group NN cord-279980-49yv65gm 73 3 was be VBD cord-279980-49yv65gm 73 4 washed wash VBN cord-279980-49yv65gm 73 5 with with IN cord-279980-49yv65gm 73 6 10 10 CD cord-279980-49yv65gm 73 7 mL ml NN cord-279980-49yv65gm 73 8 of of IN cord-279980-49yv65gm 73 9 brain brain NN cord-279980-49yv65gm 73 10 - - HYPH cord-279980-49yv65gm 73 11 heart heart NN cord-279980-49yv65gm 73 12 infusion infusion NN cord-279980-49yv65gm 73 13 ( ( -LRB- cord-279980-49yv65gm 73 14 BHI BHI NNP cord-279980-49yv65gm 73 15 ) ) -RRB- cord-279980-49yv65gm 73 16 broth broth NN cord-279980-49yv65gm 73 17 ( ( -LRB- cord-279980-49yv65gm 73 18 Difco Difco NNP cord-279980-49yv65gm 73 19 , , , cord-279980-49yv65gm 73 20 BD BD NNP cord-279980-49yv65gm 73 21 Biosciences Biosciences NNP cord-279980-49yv65gm 73 22 , , , cord-279980-49yv65gm 73 23 Sparks Sparks NNP cord-279980-49yv65gm 73 24 , , , cord-279980-49yv65gm 73 25 MD MD NNP cord-279980-49yv65gm 73 26 ) ) -RRB- cord-279980-49yv65gm 73 27 by by IN cord-279980-49yv65gm 73 28 vortexing vortexe VBG cord-279980-49yv65gm 73 29 for for IN cord-279980-49yv65gm 73 30 5 5 CD cord-279980-49yv65gm 73 31 s. s. NN cord-279980-49yv65gm 74 1 The the DT cord-279980-49yv65gm 74 2 BHI BHI NNP cord-279980-49yv65gm 74 3 washing washing NN cord-279980-49yv65gm 74 4 fluid fluid NN cord-279980-49yv65gm 74 5 ( ( -LRB- cord-279980-49yv65gm 74 6 W1 W1 NNP cord-279980-49yv65gm 74 7 ) ) -RRB- cord-279980-49yv65gm 74 8 was be VBD cord-279980-49yv65gm 74 9 inoculated inoculate VBN cord-279980-49yv65gm 74 10 into into IN cord-279980-49yv65gm 74 11 six six CD cord-279980-49yv65gm 74 12 10-day 10-day CD cord-279980-49yv65gm 74 13 - - HYPH cord-279980-49yv65gm 74 14 old old JJ cord-279980-49yv65gm 74 15 embryonated embryonate VBN cord-279980-49yv65gm 74 16 chicken chicken NN cord-279980-49yv65gm 74 17 eggs egg NNS cord-279980-49yv65gm 74 18 to to TO cord-279980-49yv65gm 74 19 evaluate evaluate VB cord-279980-49yv65gm 74 20 the the DT cord-279980-49yv65gm 74 21 presence presence NN cord-279980-49yv65gm 74 22 of of IN cord-279980-49yv65gm 74 23 the the DT cord-279980-49yv65gm 74 24 AI AI NNP cord-279980-49yv65gm 74 25 H5N1 H5N1 NNP cord-279980-49yv65gm 74 26 virus virus NN cord-279980-49yv65gm 74 27 on on IN cord-279980-49yv65gm 74 28 the the DT cord-279980-49yv65gm 74 29 external external JJ cord-279980-49yv65gm 74 30 surface surface NN cord-279980-49yv65gm 74 31 of of IN cord-279980-49yv65gm 74 32 the the DT cord-279980-49yv65gm 74 33 house house NN cord-279980-49yv65gm 74 34 flies fly VBZ cord-279980-49yv65gm 74 35 . . . cord-279980-49yv65gm 75 1 To to TO cord-279980-49yv65gm 75 2 assess assess VB cord-279980-49yv65gm 75 3 the the DT cord-279980-49yv65gm 75 4 AI AI NNP cord-279980-49yv65gm 75 5 H5N1 H5N1 NNP cord-279980-49yv65gm 75 6 virus virus NN cord-279980-49yv65gm 75 7 within within IN cord-279980-49yv65gm 75 8 the the DT cord-279980-49yv65gm 75 9 internal internal JJ cord-279980-49yv65gm 75 10 viscera viscera NN cord-279980-49yv65gm 75 11 of of IN cord-279980-49yv65gm 75 12 house house NN cord-279980-49yv65gm 75 13 flies fly NNS cord-279980-49yv65gm 75 14 , , , cord-279980-49yv65gm 75 15 the the DT cord-279980-49yv65gm 75 16 previously previously RB cord-279980-49yv65gm 75 17 washed wash VBN cord-279980-49yv65gm 75 18 flies fly NNS cord-279980-49yv65gm 75 19 were be VBD cord-279980-49yv65gm 75 20 disinfected disinfect VBN cord-279980-49yv65gm 75 21 by by IN cord-279980-49yv65gm 75 22 immersing immerse VBG cord-279980-49yv65gm 75 23 into into IN cord-279980-49yv65gm 75 24 10 10 CD cord-279980-49yv65gm 75 25 mL ml NN cord-279980-49yv65gm 75 26 of of IN cord-279980-49yv65gm 75 27 70 70 CD cord-279980-49yv65gm 75 28 % % NN cord-279980-49yv65gm 75 29 ethanol ethanol NN cord-279980-49yv65gm 75 30 , , , cord-279980-49yv65gm 75 31 vortexed vortexe VBN cord-279980-49yv65gm 75 32 for for IN cord-279980-49yv65gm 75 33 5 5 CD cord-279980-49yv65gm 75 34 s s NNS cord-279980-49yv65gm 75 35 and and CC cord-279980-49yv65gm 75 36 rinsed rinse VBD cord-279980-49yv65gm 75 37 with with IN cord-279980-49yv65gm 75 38 10 10 CD cord-279980-49yv65gm 75 39 mL ml NN cord-279980-49yv65gm 75 40 of of IN cord-279980-49yv65gm 75 41 BHI BHI NNP cord-279980-49yv65gm 75 42 washing washing NN cord-279980-49yv65gm 75 43 fluid fluid NN cord-279980-49yv65gm 75 44 ( ( -LRB- cord-279980-49yv65gm 75 45 W2 W2 NNP cord-279980-49yv65gm 75 46 ) ) -RRB- cord-279980-49yv65gm 75 47 . . . cord-279980-49yv65gm 76 1 The the DT cord-279980-49yv65gm 76 2 W2 W2 NNP cord-279980-49yv65gm 76 3 washing washing NN cord-279980-49yv65gm 76 4 fluid fluid NN cord-279980-49yv65gm 76 5 was be VBD cord-279980-49yv65gm 76 6 inoculated inoculate VBN cord-279980-49yv65gm 76 7 into into IN cord-279980-49yv65gm 76 8 six six CD cord-279980-49yv65gm 76 9 10-dayold 10-dayold CD cord-279980-49yv65gm 76 10 embryonated embryonate VBN cord-279980-49yv65gm 76 11 chicken chicken NN cord-279980-49yv65gm 76 12 eggs egg NNS cord-279980-49yv65gm 76 13 to to TO cord-279980-49yv65gm 76 14 confirm confirm VB cord-279980-49yv65gm 76 15 the the DT cord-279980-49yv65gm 76 16 absence absence NN cord-279980-49yv65gm 76 17 of of IN cord-279980-49yv65gm 76 18 the the DT cord-279980-49yv65gm 76 19 AI AI NNP cord-279980-49yv65gm 76 20 H5N1 H5N1 NNP cord-279980-49yv65gm 76 21 virus virus NN cord-279980-49yv65gm 76 22 on on IN cord-279980-49yv65gm 76 23 the the DT cord-279980-49yv65gm 76 24 external external JJ cord-279980-49yv65gm 76 25 surface surface NN cord-279980-49yv65gm 76 26 of of IN cord-279980-49yv65gm 76 27 house house NN cord-279980-49yv65gm 76 28 flies fly NNS cord-279980-49yv65gm 76 29 . . . cord-279980-49yv65gm 77 1 The the DT cord-279980-49yv65gm 77 2 washed wash VBN cord-279980-49yv65gm 77 3 house house NN cord-279980-49yv65gm 77 4 flies fly NNS cord-279980-49yv65gm 77 5 were be VBD cord-279980-49yv65gm 77 6 then then RB cord-279980-49yv65gm 77 7 homogenized homogenize VBN cord-279980-49yv65gm 77 8 with with IN cord-279980-49yv65gm 77 9 900 900 CD cord-279980-49yv65gm 77 10 μL μL NNS cord-279980-49yv65gm 77 11 of of IN cord-279980-49yv65gm 77 12 sterile sterile JJ cord-279980-49yv65gm 77 13 BHI BHI NNP cord-279980-49yv65gm 77 14 using use VBG cord-279980-49yv65gm 77 15 sterile sterile JJ cord-279980-49yv65gm 77 16 plastic plastic JJ cord-279980-49yv65gm 77 17 pestles pestle NNS cord-279980-49yv65gm 77 18 and and CC cord-279980-49yv65gm 77 19 sterile sterile JJ cord-279980-49yv65gm 77 20 microcentrifuge microcentrifuge NN cord-279980-49yv65gm 77 21 tubes tube NNS cord-279980-49yv65gm 77 22 ( ( -LRB- cord-279980-49yv65gm 77 23 Eppendorf Eppendorf NNP cord-279980-49yv65gm 77 24 South South NNP cord-279980-49yv65gm 77 25 Asia Asia NNP cord-279980-49yv65gm 77 26 , , , cord-279980-49yv65gm 77 27 Malaysia Malaysia NNP cord-279980-49yv65gm 77 28 ) ) -RRB- cord-279980-49yv65gm 77 29 and and CC cord-279980-49yv65gm 77 30 centrifuged centrifuge VBD cord-279980-49yv65gm 77 31 at at IN cord-279980-49yv65gm 77 32 600 600 CD cord-279980-49yv65gm 77 33 g g NN cord-279980-49yv65gm 77 34 for for IN cord-279980-49yv65gm 77 35 15 15 CD cord-279980-49yv65gm 77 36 min min NN cord-279980-49yv65gm 77 37 at at IN cord-279980-49yv65gm 77 38 4 4 CD cord-279980-49yv65gm 78 1 • • NNP cord-279980-49yv65gm 79 1 C. C. NNP cord-279980-49yv65gm 80 1 The the DT cord-279980-49yv65gm 80 2 supernatants supernatant NNS cord-279980-49yv65gm 80 3 were be VBD cord-279980-49yv65gm 80 4 collected collect VBN cord-279980-49yv65gm 80 5 and and CC cord-279980-49yv65gm 80 6 gentamicin gentamicin NNS cord-279980-49yv65gm 80 7 was be VBD cord-279980-49yv65gm 80 8 added add VBN cord-279980-49yv65gm 80 9 to to IN cord-279980-49yv65gm 80 10 a a DT cord-279980-49yv65gm 80 11 final final JJ cord-279980-49yv65gm 80 12 concentration concentration NN cord-279980-49yv65gm 80 13 of of IN cord-279980-49yv65gm 80 14 200 200 CD cord-279980-49yv65gm 80 15 μg μg NN cord-279980-49yv65gm 80 16 / / SYM cord-279980-49yv65gm 80 17 mL ml NN cord-279980-49yv65gm 80 18 and and CC cord-279980-49yv65gm 80 19 incubated incubate VBD cord-279980-49yv65gm 80 20 at at IN cord-279980-49yv65gm 80 21 4 4 CD cord-279980-49yv65gm 80 22 • • NN cord-279980-49yv65gm 80 23 C c NN cord-279980-49yv65gm 80 24 for for IN cord-279980-49yv65gm 80 25 40 40 CD cord-279980-49yv65gm 80 26 min min NN cord-279980-49yv65gm 80 27 . . . cord-279980-49yv65gm 81 1 Subsequently subsequently RB cord-279980-49yv65gm 81 2 , , , cord-279980-49yv65gm 81 3 virus virus NN cord-279980-49yv65gm 81 4 isolation isolation NN cord-279980-49yv65gm 81 5 was be VBD cord-279980-49yv65gm 81 6 performed perform VBN cord-279980-49yv65gm 81 7 by by IN cord-279980-49yv65gm 81 8 inoculating inoculate VBG cord-279980-49yv65gm 81 9 100 100 CD cord-279980-49yv65gm 81 10 μL μL NNS cord-279980-49yv65gm 81 11 of of IN cord-279980-49yv65gm 81 12 the the DT cord-279980-49yv65gm 81 13 supernatant supernatant NN cord-279980-49yv65gm 81 14 into into IN cord-279980-49yv65gm 81 15 six six CD cord-279980-49yv65gm 81 16 10-day 10-day CD cord-279980-49yv65gm 81 17 - - HYPH cord-279980-49yv65gm 81 18 old old JJ cord-279980-49yv65gm 81 19 embryonated embryonate VBN cord-279980-49yv65gm 81 20 chicken chicken NN cord-279980-49yv65gm 81 21 eggs egg NNS cord-279980-49yv65gm 81 22 . . . cord-279980-49yv65gm 82 1 The the DT cord-279980-49yv65gm 82 2 embryos embryo NNS cord-279980-49yv65gm 82 3 were be VBD cord-279980-49yv65gm 82 4 observed observe VBN cord-279980-49yv65gm 82 5 twice twice PDT cord-279980-49yv65gm 82 6 a a DT cord-279980-49yv65gm 82 7 day day NN cord-279980-49yv65gm 82 8 for for IN cord-279980-49yv65gm 82 9 5 5 CD cord-279980-49yv65gm 82 10 days day NNS cord-279980-49yv65gm 82 11 post post NN cord-279980-49yv65gm 82 12 - - NN cord-279980-49yv65gm 82 13 inoculation inoculation NN cord-279980-49yv65gm 82 14 . . . cord-279980-49yv65gm 83 1 The the DT cord-279980-49yv65gm 83 2 embryos embryo NNS cord-279980-49yv65gm 83 3 that that WDT cord-279980-49yv65gm 83 4 died die VBD cord-279980-49yv65gm 83 5 within within IN cord-279980-49yv65gm 83 6 24 24 CD cord-279980-49yv65gm 83 7 h h NNP cord-279980-49yv65gm 83 8 were be VBD cord-279980-49yv65gm 83 9 discarded discard VBN cord-279980-49yv65gm 83 10 . . . cord-279980-49yv65gm 84 1 Allantoic Allantoic NNP cord-279980-49yv65gm 84 2 fluid fluid NN cord-279980-49yv65gm 84 3 was be VBD cord-279980-49yv65gm 84 4 harvested harvest VBN cord-279980-49yv65gm 84 5 from from IN cord-279980-49yv65gm 84 6 eggs egg NNS cord-279980-49yv65gm 84 7 and and CC cord-279980-49yv65gm 84 8 AI AI NNP cord-279980-49yv65gm 84 9 H5N1 H5N1 NNP cord-279980-49yv65gm 84 10 virus virus NN cord-279980-49yv65gm 84 11 identification identification NN cord-279980-49yv65gm 84 12 was be VBD cord-279980-49yv65gm 84 13 performed perform VBN cord-279980-49yv65gm 84 14 using use VBG cord-279980-49yv65gm 84 15 the the DT cord-279980-49yv65gm 84 16 HA HA NNP cord-279980-49yv65gm 84 17 test test NN cord-279980-49yv65gm 84 18 and and CC cord-279980-49yv65gm 84 19 RT RT NNP cord-279980-49yv65gm 84 20 - - HYPH cord-279980-49yv65gm 84 21 PCR PCR NNP cord-279980-49yv65gm 84 22 assay assay NN cord-279980-49yv65gm 84 23 . . . cord-279980-49yv65gm 85 1 Experiment experiment NN cord-279980-49yv65gm 85 2 2 2 CD cord-279980-49yv65gm 85 3 . . . cord-279980-49yv65gm 86 1 This this DT cord-279980-49yv65gm 86 2 experiment experiment NN cord-279980-49yv65gm 86 3 was be VBD cord-279980-49yv65gm 86 4 done do VBN cord-279980-49yv65gm 86 5 to to TO cord-279980-49yv65gm 86 6 determine determine VB cord-279980-49yv65gm 86 7 if if IN cord-279980-49yv65gm 86 8 one one CD cord-279980-49yv65gm 86 9 fly fly NN cord-279980-49yv65gm 86 10 could could MD cord-279980-49yv65gm 86 11 harbor harbor VB cord-279980-49yv65gm 86 12 the the DT cord-279980-49yv65gm 86 13 virus virus NN cord-279980-49yv65gm 86 14 . . . cord-279980-49yv65gm 87 1 Experiments experiment NNS cord-279980-49yv65gm 87 2 were be VBD cord-279980-49yv65gm 87 3 performed perform VBN cord-279980-49yv65gm 87 4 in in IN cord-279980-49yv65gm 87 5 five five CD cord-279980-49yv65gm 87 6 replicates replicate NNS cord-279980-49yv65gm 87 7 . . . cord-279980-49yv65gm 88 1 Only only RB cord-279980-49yv65gm 88 2 one one CD cord-279980-49yv65gm 88 3 house house NN cord-279980-49yv65gm 88 4 fly fly NN cord-279980-49yv65gm 88 5 was be VBD cord-279980-49yv65gm 88 6 transferred transfer VBN cord-279980-49yv65gm 88 7 into into IN cord-279980-49yv65gm 88 8 a a DT cord-279980-49yv65gm 88 9 plastic plastic JJ cord-279980-49yv65gm 88 10 box box NN cord-279980-49yv65gm 88 11 and and CC cord-279980-49yv65gm 88 12 exposed expose VBN cord-279980-49yv65gm 88 13 to to IN cord-279980-49yv65gm 88 14 the the DT cord-279980-49yv65gm 88 15 mixture mixture NN cord-279980-49yv65gm 88 16 containing contain VBG cord-279980-49yv65gm 88 17 the the DT cord-279980-49yv65gm 88 18 AI AI NNP cord-279980-49yv65gm 88 19 H5N1 H5N1 NNP cord-279980-49yv65gm 88 20 virus virus NN cord-279980-49yv65gm 88 21 or or CC cord-279980-49yv65gm 88 22 without without IN cord-279980-49yv65gm 88 23 in in IN cord-279980-49yv65gm 88 24 a a DT cord-279980-49yv65gm 88 25 sterile sterile JJ cord-279980-49yv65gm 88 26 cotton cotton NN cord-279980-49yv65gm 88 27 pad pad NN cord-279980-49yv65gm 88 28 for for IN cord-279980-49yv65gm 88 29 15 15 CD cord-279980-49yv65gm 88 30 min min NN cord-279980-49yv65gm 88 31 as as IN cord-279980-49yv65gm 88 32 previously previously RB cord-279980-49yv65gm 88 33 described describe VBN cord-279980-49yv65gm 88 34 in in IN cord-279980-49yv65gm 88 35 experiment experiment NN cord-279980-49yv65gm 88 36 1 1 CD cord-279980-49yv65gm 88 37 . . . cord-279980-49yv65gm 89 1 Subsequently subsequently RB cord-279980-49yv65gm 89 2 , , , cord-279980-49yv65gm 89 3 the the DT cord-279980-49yv65gm 89 4 exposed expose VBN cord-279980-49yv65gm 89 5 house house NN cord-279980-49yv65gm 89 6 fly fly NN cord-279980-49yv65gm 89 7 was be VBD cord-279980-49yv65gm 89 8 washed wash VBN cord-279980-49yv65gm 89 9 with with IN cord-279980-49yv65gm 89 10 1 1 CD cord-279980-49yv65gm 89 11 mL ml NN cord-279980-49yv65gm 89 12 of of IN cord-279980-49yv65gm 89 13 BHI BHI NNP cord-279980-49yv65gm 89 14 as as IN cord-279980-49yv65gm 89 15 W1 w1 NN cord-279980-49yv65gm 89 16 washing washing NN cord-279980-49yv65gm 89 17 fluid fluid NN cord-279980-49yv65gm 89 18 . . . cord-279980-49yv65gm 90 1 The the DT cord-279980-49yv65gm 90 2 W1 w1 NN cord-279980-49yv65gm 90 3 washing washing NN cord-279980-49yv65gm 90 4 fluid fluid NN cord-279980-49yv65gm 90 5 was be VBD cord-279980-49yv65gm 90 6 titrated titrate VBN cord-279980-49yv65gm 90 7 by by IN cord-279980-49yv65gm 90 8 inoculation inoculation NN cord-279980-49yv65gm 90 9 into into IN cord-279980-49yv65gm 90 10 a a DT cord-279980-49yv65gm 90 11 10-dayold 10-dayold CD cord-279980-49yv65gm 90 12 embryonated embryonate VBN cord-279980-49yv65gm 90 13 chicken chicken NN cord-279980-49yv65gm 90 14 and and CC cord-279980-49yv65gm 90 15 the the DT cord-279980-49yv65gm 90 16 W1 W1 NNP cord-279980-49yv65gm 90 17 washing washing NN cord-279980-49yv65gm 90 18 fluid fluid NN cord-279980-49yv65gm 90 19 titre titre NN cord-279980-49yv65gm 90 20 was be VBD cord-279980-49yv65gm 90 21 compared compare VBN cord-279980-49yv65gm 90 22 with with IN cord-279980-49yv65gm 90 23 the the DT cord-279980-49yv65gm 90 24 virus virus NN cord-279980-49yv65gm 90 25 titre titre NN cord-279980-49yv65gm 90 26 in in IN cord-279980-49yv65gm 90 27 fly fly NN cord-279980-49yv65gm 90 28 homogenate homogenate NNP cord-279980-49yv65gm 90 29 . . . cord-279980-49yv65gm 91 1 The the DT cord-279980-49yv65gm 91 2 steps step NNS cord-279980-49yv65gm 91 3 for for IN cord-279980-49yv65gm 91 4 virus virus NN cord-279980-49yv65gm 91 5 isolation isolation NN cord-279980-49yv65gm 91 6 and and CC cord-279980-49yv65gm 91 7 identification identification NN cord-279980-49yv65gm 91 8 were be VBD cord-279980-49yv65gm 91 9 performed perform VBN cord-279980-49yv65gm 91 10 as as IN cord-279980-49yv65gm 91 11 previously previously RB cord-279980-49yv65gm 91 12 described describe VBN cord-279980-49yv65gm 91 13 in in IN cord-279980-49yv65gm 91 14 experiment experiment NN cord-279980-49yv65gm 91 15 1 1 CD cord-279980-49yv65gm 91 16 . . . cord-279980-49yv65gm 92 1 Experiment experiment NN cord-279980-49yv65gm 92 2 3 3 CD cord-279980-49yv65gm 92 3 . . . cord-279980-49yv65gm 93 1 This this DT cord-279980-49yv65gm 93 2 experiment experiment NN cord-279980-49yv65gm 93 3 aimed aim VBN cord-279980-49yv65gm 93 4 to to TO cord-279980-49yv65gm 93 5 evaluate evaluate VB cord-279980-49yv65gm 93 6 the the DT cord-279980-49yv65gm 93 7 correlation correlation NN cord-279980-49yv65gm 93 8 between between IN cord-279980-49yv65gm 93 9 the the DT cord-279980-49yv65gm 93 10 time time NN cord-279980-49yv65gm 93 11 period period NN cord-279980-49yv65gm 93 12 after after IN cord-279980-49yv65gm 93 13 exposure exposure NN cord-279980-49yv65gm 93 14 and and CC cord-279980-49yv65gm 93 15 AI AI NNP cord-279980-49yv65gm 93 16 H5N1 H5N1 NNP cord-279980-49yv65gm 93 17 virus virus NN cord-279980-49yv65gm 93 18 viability viability NN cord-279980-49yv65gm 93 19 in in IN cord-279980-49yv65gm 93 20 AI AI NNP cord-279980-49yv65gm 93 21 H5N1 H5N1 NNP cord-279980-49yv65gm 93 22 exposed expose VBD cord-279980-49yv65gm 93 23 house house NN cord-279980-49yv65gm 93 24 flies fly NNS cord-279980-49yv65gm 93 25 . . . cord-279980-49yv65gm 94 1 The the DT cord-279980-49yv65gm 94 2 house house NN cord-279980-49yv65gm 94 3 flies fly NNS cord-279980-49yv65gm 94 4 were be VBD cord-279980-49yv65gm 94 5 separated separate VBN cord-279980-49yv65gm 94 6 into into IN cord-279980-49yv65gm 94 7 two two CD cord-279980-49yv65gm 94 8 groups group NNS cord-279980-49yv65gm 94 9 ; ; : cord-279980-49yv65gm 94 10 each each DT cord-279980-49yv65gm 94 11 group group NN cord-279980-49yv65gm 94 12 was be VBD cord-279980-49yv65gm 94 13 exposed expose VBN cord-279980-49yv65gm 94 14 to to IN cord-279980-49yv65gm 94 15 the the DT cord-279980-49yv65gm 94 16 mixture mixture NN cord-279980-49yv65gm 94 17 containing contain VBG cord-279980-49yv65gm 94 18 the the DT cord-279980-49yv65gm 94 19 AI AI NNP cord-279980-49yv65gm 94 20 H5N1 H5N1 NNP cord-279980-49yv65gm 94 21 virus virus NN cord-279980-49yv65gm 94 22 or or CC cord-279980-49yv65gm 94 23 without without IN cord-279980-49yv65gm 94 24 as as RB cord-279980-49yv65gm 94 25 previously previously RB cord-279980-49yv65gm 94 26 described describe VBN cord-279980-49yv65gm 94 27 . . . cord-279980-49yv65gm 95 1 After after IN cord-279980-49yv65gm 95 2 exposure exposure NN cord-279980-49yv65gm 95 3 to to IN cord-279980-49yv65gm 95 4 the the DT cord-279980-49yv65gm 95 5 virus virus NN cord-279980-49yv65gm 95 6 , , , cord-279980-49yv65gm 95 7 standard standard JJ cord-279980-49yv65gm 95 8 food food NN cord-279980-49yv65gm 95 9 ( ( -LRB- cord-279980-49yv65gm 95 10 UHT UHT NNP cord-279980-49yv65gm 95 11 milk milk NN cord-279980-49yv65gm 95 12 mixed mix VBN cord-279980-49yv65gm 95 13 with with IN cord-279980-49yv65gm 95 14 10 10 CD cord-279980-49yv65gm 95 15 % % NN cord-279980-49yv65gm 95 16 sucrose sucrose NN cord-279980-49yv65gm 95 17 ) ) -RRB- cord-279980-49yv65gm 95 18 was be VBD cord-279980-49yv65gm 95 19 provided provide VBN cord-279980-49yv65gm 95 20 for for IN cord-279980-49yv65gm 95 21 all all DT cord-279980-49yv65gm 95 22 groups group NNS cord-279980-49yv65gm 95 23 throughout throughout IN cord-279980-49yv65gm 95 24 experiment experiment NN cord-279980-49yv65gm 95 25 and and CC cord-279980-49yv65gm 96 1 five five CD cord-279980-49yv65gm 96 2 AI AI NNP cord-279980-49yv65gm 96 3 H5N1 H5N1 NNP cord-279980-49yv65gm 96 4 exposed expose VBD cord-279980-49yv65gm 96 5 house house NN cord-279980-49yv65gm 96 6 flies fly VBZ cord-279980-49yv65gm 96 7 from from IN cord-279980-49yv65gm 96 8 each each DT cord-279980-49yv65gm 96 9 group group NN cord-279980-49yv65gm 96 10 were be VBD cord-279980-49yv65gm 96 11 examined examine VBN cord-279980-49yv65gm 96 12 for for IN cord-279980-49yv65gm 96 13 the the DT cord-279980-49yv65gm 96 14 presence presence NN cord-279980-49yv65gm 96 15 of of IN cord-279980-49yv65gm 96 16 the the DT cord-279980-49yv65gm 96 17 AI AI NNP cord-279980-49yv65gm 96 18 H5N1 H5N1 NNP cord-279980-49yv65gm 96 19 virus virus NN cord-279980-49yv65gm 96 20 at at IN cord-279980-49yv65gm 96 21 0 0 CD cord-279980-49yv65gm 96 22 , , , cord-279980-49yv65gm 96 23 6 6 CD cord-279980-49yv65gm 96 24 , , , cord-279980-49yv65gm 96 25 12 12 CD cord-279980-49yv65gm 96 26 , , , cord-279980-49yv65gm 96 27 24 24 CD cord-279980-49yv65gm 96 28 , , , cord-279980-49yv65gm 96 29 36 36 CD cord-279980-49yv65gm 96 30 , , , cord-279980-49yv65gm 96 31 48 48 CD cord-279980-49yv65gm 96 32 , , , cord-279980-49yv65gm 96 33 72 72 CD cord-279980-49yv65gm 96 34 and and CC cord-279980-49yv65gm 96 35 96 96 CD cord-279980-49yv65gm 96 36 h h NN cord-279980-49yv65gm 96 37 postexposure postexposure NN cord-279980-49yv65gm 96 38 . . . cord-279980-49yv65gm 97 1 The the DT cord-279980-49yv65gm 97 2 AI AI NNP cord-279980-49yv65gm 97 3 H5N1-exposed H5N1-exposed NNP cord-279980-49yv65gm 97 4 flies fly NNS cord-279980-49yv65gm 97 5 collected collect VBN cord-279980-49yv65gm 97 6 from from IN cord-279980-49yv65gm 97 7 each each DT cord-279980-49yv65gm 97 8 time time NN cord-279980-49yv65gm 97 9 period period NN cord-279980-49yv65gm 97 10 were be VBD cord-279980-49yv65gm 97 11 washed wash VBN cord-279980-49yv65gm 97 12 three three CD cord-279980-49yv65gm 97 13 times time NNS cord-279980-49yv65gm 97 14 and and CC cord-279980-49yv65gm 97 15 homogenized homogenize VBN cord-279980-49yv65gm 97 16 as as IN cord-279980-49yv65gm 97 17 previously previously RB cord-279980-49yv65gm 97 18 described describe VBN cord-279980-49yv65gm 97 19 , , , cord-279980-49yv65gm 97 20 to to TO cord-279980-49yv65gm 97 21 determine determine VB cord-279980-49yv65gm 97 22 the the DT cord-279980-49yv65gm 97 23 presence presence NN cord-279980-49yv65gm 97 24 of of IN cord-279980-49yv65gm 97 25 AI AI NNP cord-279980-49yv65gm 97 26 H5N1 H5N1 NNP cord-279980-49yv65gm 97 27 viruses virus NNS cord-279980-49yv65gm 97 28 . . . cord-279980-49yv65gm 98 1 The the DT cord-279980-49yv65gm 98 2 AI AI NNP cord-279980-49yv65gm 98 3 H5N1 H5N1 NNP cord-279980-49yv65gm 98 4 virus virus NN cord-279980-49yv65gm 98 5 was be VBD cord-279980-49yv65gm 98 6 isolated isolate VBN cord-279980-49yv65gm 98 7 by by IN cord-279980-49yv65gm 98 8 inoculating inoculate VBG cord-279980-49yv65gm 98 9 six six CD cord-279980-49yv65gm 98 10 10-dayold 10-dayold CD cord-279980-49yv65gm 98 11 embryonated embryonate VBN cord-279980-49yv65gm 98 12 chicken chicken NN cord-279980-49yv65gm 98 13 eggs egg NNS cord-279980-49yv65gm 98 14 as as IN cord-279980-49yv65gm 98 15 previously previously RB cord-279980-49yv65gm 98 16 described describe VBN cord-279980-49yv65gm 98 17 ( ( -LRB- cord-279980-49yv65gm 98 18 OIE OIE NNP cord-279980-49yv65gm 98 19 , , , cord-279980-49yv65gm 98 20 2000 2000 CD cord-279980-49yv65gm 98 21 ) ) -RRB- cord-279980-49yv65gm 98 22 . . . cord-279980-49yv65gm 99 1 Briefly briefly RB cord-279980-49yv65gm 99 2 , , , cord-279980-49yv65gm 99 3 100 100 CD cord-279980-49yv65gm 99 4 μL μL NNS cord-279980-49yv65gm 99 5 of of IN cord-279980-49yv65gm 99 6 10-fold 10-fold JJ cord-279980-49yv65gm 99 7 , , , cord-279980-49yv65gm 99 8 serially serially RB cord-279980-49yv65gm 99 9 diluted dilute VBN cord-279980-49yv65gm 99 10 supernatant supernatant NN cord-279980-49yv65gm 99 11 of of IN cord-279980-49yv65gm 99 12 house house NN cord-279980-49yv65gm 99 13 fly fly NN cord-279980-49yv65gm 99 14 homogenates homogenate NNS cord-279980-49yv65gm 99 15 was be VBD cord-279980-49yv65gm 99 16 inoculated inoculate VBN cord-279980-49yv65gm 99 17 into into IN cord-279980-49yv65gm 99 18 the the DT cord-279980-49yv65gm 99 19 allantoic allantoic NNP cord-279980-49yv65gm 99 20 cavity cavity NN cord-279980-49yv65gm 99 21 of of IN cord-279980-49yv65gm 99 22 six six CD cord-279980-49yv65gm 99 23 10-day 10-day CD cord-279980-49yv65gm 99 24 - - HYPH cord-279980-49yv65gm 99 25 old old JJ cord-279980-49yv65gm 99 26 embryonated embryonate VBN cord-279980-49yv65gm 99 27 chicken chicken NN cord-279980-49yv65gm 99 28 eggs egg NNS cord-279980-49yv65gm 99 29 . . . cord-279980-49yv65gm 100 1 Embryos embryo NNS cord-279980-49yv65gm 100 2 were be VBD cord-279980-49yv65gm 100 3 examined examine VBN cord-279980-49yv65gm 100 4 twice twice PDT cord-279980-49yv65gm 100 5 a a DT cord-279980-49yv65gm 100 6 day day NN cord-279980-49yv65gm 100 7 for for IN cord-279980-49yv65gm 100 8 5 5 CD cord-279980-49yv65gm 100 9 days day NNS cord-279980-49yv65gm 100 10 and and CC cord-279980-49yv65gm 100 11 allantoic allantoic NNP cord-279980-49yv65gm 100 12 fluid fluid NN cord-279980-49yv65gm 100 13 was be VBD cord-279980-49yv65gm 100 14 harvested harvest VBN cord-279980-49yv65gm 100 15 at at IN cord-279980-49yv65gm 100 16 5 5 CD cord-279980-49yv65gm 100 17 days day NNS cord-279980-49yv65gm 100 18 post post NN cord-279980-49yv65gm 100 19 - - NN cord-279980-49yv65gm 100 20 inoculation inoculation JJ cord-279980-49yv65gm 100 21 or or CC cord-279980-49yv65gm 100 22 upon upon IN cord-279980-49yv65gm 100 23 embryo embryo NN cord-279980-49yv65gm 100 24 death death NN cord-279980-49yv65gm 100 25 . . . cord-279980-49yv65gm 101 1 The the DT cord-279980-49yv65gm 101 2 HA HA NNP cord-279980-49yv65gm 101 3 test test NN cord-279980-49yv65gm 101 4 and and CC cord-279980-49yv65gm 101 5 the the DT cord-279980-49yv65gm 101 6 RT RT NNP cord-279980-49yv65gm 101 7 - - HYPH cord-279980-49yv65gm 101 8 PCR PCR NNP cord-279980-49yv65gm 101 9 assay assay NN cord-279980-49yv65gm 101 10 were be VBD cord-279980-49yv65gm 101 11 performed perform VBN cord-279980-49yv65gm 101 12 to to TO cord-279980-49yv65gm 101 13 determine determine VB cord-279980-49yv65gm 101 14 the the DT cord-279980-49yv65gm 101 15 presence presence NN cord-279980-49yv65gm 101 16 of of IN cord-279980-49yv65gm 101 17 the the DT cord-279980-49yv65gm 101 18 virus virus NN cord-279980-49yv65gm 101 19 . . . cord-279980-49yv65gm 102 1 Virus virus NN cord-279980-49yv65gm 102 2 titre titre NN cord-279980-49yv65gm 102 3 was be VBD cord-279980-49yv65gm 102 4 determined determine VBN cord-279980-49yv65gm 102 5 according accord VBG cord-279980-49yv65gm 102 6 to to IN cord-279980-49yv65gm 102 7 the the DT cord-279980-49yv65gm 102 8 method method NN cord-279980-49yv65gm 102 9 of of IN cord-279980-49yv65gm 102 10 Reed Reed NNP cord-279980-49yv65gm 102 11 & & CC cord-279980-49yv65gm 102 12 Muench Muench NNP cord-279980-49yv65gm 102 13 ( ( -LRB- cord-279980-49yv65gm 102 14 1938 1938 CD cord-279980-49yv65gm 102 15 ) ) -RRB- cord-279980-49yv65gm 102 16 . . . cord-279980-49yv65gm 103 1 Allantoic Allantoic NNP cord-279980-49yv65gm 103 2 fluid fluid NN cord-279980-49yv65gm 103 3 was be VBD cord-279980-49yv65gm 103 4 harvested harvest VBN cord-279980-49yv65gm 103 5 from from IN cord-279980-49yv65gm 103 6 each each DT cord-279980-49yv65gm 103 7 embryonated embryonate VBN cord-279980-49yv65gm 103 8 chicken chicken NN cord-279980-49yv65gm 103 9 egg egg NN cord-279980-49yv65gm 103 10 and and CC cord-279980-49yv65gm 103 11 the the DT cord-279980-49yv65gm 103 12 HA HA NNP cord-279980-49yv65gm 103 13 test test NN cord-279980-49yv65gm 103 14 was be VBD cord-279980-49yv65gm 103 15 performed perform VBN cord-279980-49yv65gm 103 16 using use VBG cord-279980-49yv65gm 103 17 a a DT cord-279980-49yv65gm 103 18 standard standard JJ cord-279980-49yv65gm 103 19 protocol protocol NN cord-279980-49yv65gm 103 20 ( ( -LRB- cord-279980-49yv65gm 103 21 OIE OIE NNP cord-279980-49yv65gm 103 22 , , , cord-279980-49yv65gm 103 23 2000 2000 CD cord-279980-49yv65gm 103 24 ) ) -RRB- cord-279980-49yv65gm 103 25 . . . cord-279980-49yv65gm 104 1 Briefly briefly RB cord-279980-49yv65gm 104 2 , , , cord-279980-49yv65gm 104 3 25 25 CD cord-279980-49yv65gm 104 4 μL μL NNS cord-279980-49yv65gm 104 5 of of IN cord-279980-49yv65gm 104 6 phosphate phosphate NN cord-279980-49yv65gm 104 7 - - HYPH cord-279980-49yv65gm 104 8 buffered buffer VBN cord-279980-49yv65gm 104 9 saline saline NN cord-279980-49yv65gm 104 10 ( ( -LRB- cord-279980-49yv65gm 104 11 PBS PBS NNP cord-279980-49yv65gm 104 12 ) ) -RRB- cord-279980-49yv65gm 104 13 was be VBD cord-279980-49yv65gm 104 14 added add VBN cord-279980-49yv65gm 104 15 into into IN cord-279980-49yv65gm 104 16 a a DT cord-279980-49yv65gm 104 17 96-well 96-well CD cord-279980-49yv65gm 104 18 , , , cord-279980-49yv65gm 104 19 V v NN cord-279980-49yv65gm 104 20 - - HYPH cord-279980-49yv65gm 104 21 shaped shape VBN cord-279980-49yv65gm 104 22 bottom bottom NN cord-279980-49yv65gm 104 23 microtitre microtitre NNP cord-279980-49yv65gm 104 24 plate plate NN cord-279980-49yv65gm 104 25 followed follow VBN cord-279980-49yv65gm 104 26 by by IN cord-279980-49yv65gm 104 27 the the DT cord-279980-49yv65gm 104 28 addition addition NN cord-279980-49yv65gm 104 29 of of IN cord-279980-49yv65gm 104 30 25 25 CD cord-279980-49yv65gm 104 31 μL μL NNS cord-279980-49yv65gm 104 32 of of IN cord-279980-49yv65gm 104 33 the the DT cord-279980-49yv65gm 104 34 samples sample NNS cord-279980-49yv65gm 104 35 . . . cord-279980-49yv65gm 105 1 The the DT cord-279980-49yv65gm 105 2 solution solution NN cord-279980-49yv65gm 105 3 was be VBD cord-279980-49yv65gm 105 4 two two CD cord-279980-49yv65gm 105 5 - - : cord-279980-49yv65gm 105 6 fold fold VB cord-279980-49yv65gm 105 7 serially serially RB cord-279980-49yv65gm 105 8 diluted dilute VBN cord-279980-49yv65gm 105 9 with with IN cord-279980-49yv65gm 105 10 PBS PBS NNP cord-279980-49yv65gm 105 11 . . . cord-279980-49yv65gm 106 1 Twenty twenty CD cord-279980-49yv65gm 106 2 - - HYPH cord-279980-49yv65gm 106 3 five five CD cord-279980-49yv65gm 106 4 microlitres microlitre NNS cord-279980-49yv65gm 106 5 of of IN cord-279980-49yv65gm 106 6 PBS PBS NNP cord-279980-49yv65gm 106 7 was be VBD cord-279980-49yv65gm 106 8 added add VBN cord-279980-49yv65gm 106 9 into into IN cord-279980-49yv65gm 106 10 all all DT cord-279980-49yv65gm 106 11 wells well NNS cord-279980-49yv65gm 106 12 followed follow VBN cord-279980-49yv65gm 106 13 by by IN cord-279980-49yv65gm 106 14 the the DT cord-279980-49yv65gm 106 15 addition addition NN cord-279980-49yv65gm 106 16 of of IN cord-279980-49yv65gm 106 17 25 25 CD cord-279980-49yv65gm 106 18 μL μL NNS cord-279980-49yv65gm 106 19 of of IN cord-279980-49yv65gm 106 20 1 1 CD cord-279980-49yv65gm 106 21 % % NN cord-279980-49yv65gm 106 22 chicken chicken NN cord-279980-49yv65gm 106 23 red red JJ cord-279980-49yv65gm 106 24 blood blood NN cord-279980-49yv65gm 106 25 cells cell NNS cord-279980-49yv65gm 106 26 ( ( -LRB- cord-279980-49yv65gm 106 27 RBCs rbc NNS cord-279980-49yv65gm 106 28 ) ) -RRB- cord-279980-49yv65gm 106 29 in in IN cord-279980-49yv65gm 106 30 PBS PBS NNP cord-279980-49yv65gm 106 31 . . . cord-279980-49yv65gm 107 1 The the DT cord-279980-49yv65gm 107 2 microtitre microtitre NNP cord-279980-49yv65gm 107 3 plate plate NN cord-279980-49yv65gm 107 4 was be VBD cord-279980-49yv65gm 107 5 gently gently RB cord-279980-49yv65gm 107 6 tapped tap VBN cord-279980-49yv65gm 107 7 and and CC cord-279980-49yv65gm 107 8 RBCs rbc NNS cord-279980-49yv65gm 107 9 were be VBD cord-279980-49yv65gm 107 10 allowed allow VBN cord-279980-49yv65gm 107 11 to to TO cord-279980-49yv65gm 107 12 settle settle VB cord-279980-49yv65gm 107 13 for for IN cord-279980-49yv65gm 107 14 40 40 CD cord-279980-49yv65gm 107 15 min min NN cord-279980-49yv65gm 107 16 at at IN cord-279980-49yv65gm 107 17 room room NN cord-279980-49yv65gm 107 18 temperature temperature NN cord-279980-49yv65gm 107 19 . . . cord-279980-49yv65gm 108 1 The the DT cord-279980-49yv65gm 108 2 AI AI NNP cord-279980-49yv65gm 108 3 H5N1 H5N1 NNP cord-279980-49yv65gm 108 4 virus virus NN cord-279980-49yv65gm 108 5 was be VBD cord-279980-49yv65gm 108 6 also also RB cord-279980-49yv65gm 108 7 included include VBN cord-279980-49yv65gm 108 8 as as IN cord-279980-49yv65gm 108 9 positive positive JJ cord-279980-49yv65gm 108 10 controls control NNS cord-279980-49yv65gm 108 11 . . . cord-279980-49yv65gm 109 1 Samples sample NNS cord-279980-49yv65gm 109 2 with with IN cord-279980-49yv65gm 109 3 the the DT cord-279980-49yv65gm 109 4 HA HA NNP cord-279980-49yv65gm 109 5 titre titre NN cord-279980-49yv65gm 109 6 ≥4 ≥4 NNP cord-279980-49yv65gm 109 7 were be VBD cord-279980-49yv65gm 109 8 considered consider VBN cord-279980-49yv65gm 109 9 positive positive JJ cord-279980-49yv65gm 109 10 for for IN cord-279980-49yv65gm 109 11 the the DT cord-279980-49yv65gm 109 12 AI AI NNP cord-279980-49yv65gm 109 13 H5N1 H5N1 NNP cord-279980-49yv65gm 109 14 virus virus NN cord-279980-49yv65gm 109 15 . . . cord-279980-49yv65gm 110 1 RNA RNA NNP cord-279980-49yv65gm 110 2 was be VBD cord-279980-49yv65gm 110 3 extracted extract VBN cord-279980-49yv65gm 110 4 from from IN cord-279980-49yv65gm 110 5 the the DT cord-279980-49yv65gm 110 6 supernatant supernatant NN cord-279980-49yv65gm 110 7 of of IN cord-279980-49yv65gm 110 8 house house NN cord-279980-49yv65gm 110 9 fly fly VB cord-279980-49yv65gm 110 10 homogenates homogenate NNS cord-279980-49yv65gm 110 11 or or CC cord-279980-49yv65gm 110 12 allantoic allantoic NNP cord-279980-49yv65gm 110 13 fluid fluid NN cord-279980-49yv65gm 110 14 using use VBG cord-279980-49yv65gm 110 15 the the DT cord-279980-49yv65gm 110 16 QIAamp QIAamp NNP cord-279980-49yv65gm 110 17 Viral Viral NNP cord-279980-49yv65gm 110 18 RNA RNA NNP cord-279980-49yv65gm 110 19 mini mini NN cord-279980-49yv65gm 110 20 kit kit NN cord-279980-49yv65gm 110 21 ( ( -LRB- cord-279980-49yv65gm 110 22 Qiagen Qiagen NNP cord-279980-49yv65gm 110 23 , , , cord-279980-49yv65gm 110 24 Hilden Hilden NNP cord-279980-49yv65gm 110 25 , , , cord-279980-49yv65gm 110 26 Germany Germany NNP cord-279980-49yv65gm 110 27 ) ) -RRB- cord-279980-49yv65gm 110 28 according accord VBG cord-279980-49yv65gm 110 29 to to IN cord-279980-49yv65gm 110 30 the the DT cord-279980-49yv65gm 110 31 manufacturer manufacturer NN cord-279980-49yv65gm 110 32 's 's POS cord-279980-49yv65gm 110 33 instructions instruction NNS cord-279980-49yv65gm 110 34 . . . cord-279980-49yv65gm 111 1 Briefly briefly RB cord-279980-49yv65gm 111 2 , , , cord-279980-49yv65gm 111 3 140 140 CD cord-279980-49yv65gm 111 4 μL μL NNS cord-279980-49yv65gm 111 5 of of IN cord-279980-49yv65gm 111 6 sample sample NN cord-279980-49yv65gm 111 7 was be VBD cord-279980-49yv65gm 111 8 mixed mix VBN cord-279980-49yv65gm 111 9 with with IN cord-279980-49yv65gm 111 10 560 560 CD cord-279980-49yv65gm 111 11 μL μL NNS cord-279980-49yv65gm 111 12 of of IN cord-279980-49yv65gm 111 13 Buffer Buffer NNP cord-279980-49yv65gm 111 14 AVL AVL NNP cord-279980-49yv65gm 111 15 containing contain VBG cord-279980-49yv65gm 111 16 carrier carrier NN cord-279980-49yv65gm 111 17 RNA RNA NNP cord-279980-49yv65gm 111 18 , , , cord-279980-49yv65gm 111 19 vortexed vortexe VBD cord-279980-49yv65gm 111 20 for for IN cord-279980-49yv65gm 111 21 15 15 CD cord-279980-49yv65gm 111 22 s s NNS cord-279980-49yv65gm 111 23 , , , cord-279980-49yv65gm 111 24 and and CC cord-279980-49yv65gm 111 25 incubated incubate VBN cord-279980-49yv65gm 111 26 at at IN cord-279980-49yv65gm 111 27 room room NN cord-279980-49yv65gm 111 28 temperature temperature NN cord-279980-49yv65gm 111 29 for for IN cord-279980-49yv65gm 111 30 10 10 CD cord-279980-49yv65gm 111 31 min min NN cord-279980-49yv65gm 111 32 . . . cord-279980-49yv65gm 112 1 Subsequently subsequently RB cord-279980-49yv65gm 112 2 , , , cord-279980-49yv65gm 112 3 560 560 CD cord-279980-49yv65gm 112 4 μL μL NNS cord-279980-49yv65gm 112 5 of of IN cord-279980-49yv65gm 112 6 absolute absolute JJ cord-279980-49yv65gm 112 7 alcohol alcohol NN cord-279980-49yv65gm 112 8 was be VBD cord-279980-49yv65gm 112 9 added add VBN cord-279980-49yv65gm 112 10 to to IN cord-279980-49yv65gm 112 11 the the DT cord-279980-49yv65gm 112 12 sample sample NN cord-279980-49yv65gm 112 13 . . . cord-279980-49yv65gm 113 1 The the DT cord-279980-49yv65gm 113 2 solution solution NN cord-279980-49yv65gm 113 3 was be VBD cord-279980-49yv65gm 113 4 transferred transfer VBN cord-279980-49yv65gm 113 5 to to IN cord-279980-49yv65gm 113 6 QIAmp QIAmp NNP cord-279980-49yv65gm 113 7 Mini Mini NNP cord-279980-49yv65gm 113 8 column column NN cord-279980-49yv65gm 113 9 and and CC cord-279980-49yv65gm 113 10 centrifuged centrifuge VBD cord-279980-49yv65gm 113 11 at at IN cord-279980-49yv65gm 113 12 11 11 CD cord-279980-49yv65gm 113 13 000 000 CD cord-279980-49yv65gm 113 14 g g NN cord-279980-49yv65gm 113 15 for for IN cord-279980-49yv65gm 113 16 1 1 CD cord-279980-49yv65gm 113 17 min min NN cord-279980-49yv65gm 113 18 . . . cord-279980-49yv65gm 114 1 Columns column NNS cord-279980-49yv65gm 114 2 were be VBD cord-279980-49yv65gm 114 3 washed wash VBN cord-279980-49yv65gm 114 4 twice twice RB cord-279980-49yv65gm 114 5 with with IN cord-279980-49yv65gm 114 6 sterile sterile JJ cord-279980-49yv65gm 114 7 buffer buffer NN cord-279980-49yv65gm 114 8 . . . cord-279980-49yv65gm 115 1 RNA RNA NNP cord-279980-49yv65gm 115 2 was be VBD cord-279980-49yv65gm 115 3 eluted elute VBN cord-279980-49yv65gm 115 4 with with IN cord-279980-49yv65gm 115 5 50 50 CD cord-279980-49yv65gm 115 6 μL μL NNS cord-279980-49yv65gm 115 7 of of IN cord-279980-49yv65gm 115 8 nuclease nuclease NN cord-279980-49yv65gm 115 9 - - HYPH cord-279980-49yv65gm 115 10 free free JJ cord-279980-49yv65gm 115 11 water water NN cord-279980-49yv65gm 115 12 . . . cord-279980-49yv65gm 116 1 The the DT cord-279980-49yv65gm 116 2 amount amount NN cord-279980-49yv65gm 116 3 of of IN cord-279980-49yv65gm 116 4 3 3 CD cord-279980-49yv65gm 116 5 and and CC cord-279980-49yv65gm 116 6 4 4 CD cord-279980-49yv65gm 116 7 μL μL NNS cord-279980-49yv65gm 116 8 of of IN cord-279980-49yv65gm 116 9 the the DT cord-279980-49yv65gm 116 10 RNA RNA NNP cord-279980-49yv65gm 116 11 template template NN cord-279980-49yv65gm 116 12 was be VBD cord-279980-49yv65gm 116 13 used use VBN cord-279980-49yv65gm 116 14 for for IN cord-279980-49yv65gm 116 15 analysis analysis NN cord-279980-49yv65gm 116 16 by by IN cord-279980-49yv65gm 116 17 RT RT NNP cord-279980-49yv65gm 116 18 - - HYPH cord-279980-49yv65gm 116 19 PCR PCR NNP cord-279980-49yv65gm 116 20 and and CC cord-279980-49yv65gm 116 21 RRT RRT NNP cord-279980-49yv65gm 116 22 - - HYPH cord-279980-49yv65gm 116 23 PCR PCR NNP cord-279980-49yv65gm 116 24 assays assay NNS cord-279980-49yv65gm 116 25 , , , cord-279980-49yv65gm 116 26 respectively respectively RB cord-279980-49yv65gm 116 27 . . . cord-279980-49yv65gm 117 1 The the DT cord-279980-49yv65gm 117 2 presence presence NN cord-279980-49yv65gm 117 3 of of IN cord-279980-49yv65gm 117 4 the the DT cord-279980-49yv65gm 117 5 HPAI HPAI NNP cord-279980-49yv65gm 117 6 H5N1 H5N1 NNP cord-279980-49yv65gm 117 7 virus virus NN cord-279980-49yv65gm 117 8 in in IN cord-279980-49yv65gm 117 9 the the DT cord-279980-49yv65gm 117 10 allantoic allantoic NNP cord-279980-49yv65gm 117 11 fluids fluid NNS cord-279980-49yv65gm 117 12 was be VBD cord-279980-49yv65gm 117 13 determined determine VBN cord-279980-49yv65gm 117 14 using use VBG cord-279980-49yv65gm 117 15 the the DT cord-279980-49yv65gm 117 16 RT RT NNP cord-279980-49yv65gm 117 17 - - HYPH cord-279980-49yv65gm 117 18 PCR PCR NNP cord-279980-49yv65gm 117 19 assay assay NN cord-279980-49yv65gm 117 20 , , , cord-279980-49yv65gm 117 21 as as IN cord-279980-49yv65gm 117 22 previously previously RB cord-279980-49yv65gm 117 23 described describe VBN cord-279980-49yv65gm 117 24 ( ( -LRB- cord-279980-49yv65gm 117 25 Payungporn Payungporn NNP cord-279980-49yv65gm 117 26 et et FW cord-279980-49yv65gm 117 27 al al NNP cord-279980-49yv65gm 117 28 . . NNP cord-279980-49yv65gm 117 29 , , , cord-279980-49yv65gm 117 30 2006 2006 CD cord-279980-49yv65gm 117 31 ) ) -RRB- cord-279980-49yv65gm 117 32 , , , cord-279980-49yv65gm 117 33 using use VBG cord-279980-49yv65gm 117 34 the the DT cord-279980-49yv65gm 117 35 M M NNP cord-279980-49yv65gm 117 36 - - HYPH cord-279980-49yv65gm 117 37 specific specific JJ cord-279980-49yv65gm 117 38 primer primer NN cord-279980-49yv65gm 117 39 set set NN cord-279980-49yv65gm 117 40 , , , cord-279980-49yv65gm 117 41 MF MF NNP cord-279980-49yv65gm 117 42 5 5 CD cord-279980-49yv65gm 117 43 -TGATCTTCTTGAAAATTTGCAG-3 -TGATCTTCTTGAAAATTTGCAG-3 NNP cord-279980-49yv65gm 117 44 and and CC cord-279980-49yv65gm 117 45 MR MR NNP cord-279980-49yv65gm 117 46 5 5 CD cord-279980-49yv65gm 117 47 -TGTTGACAAAATGACCATCG-3 -TGTTGACAAAATGACCATCG-3 NNP cord-279980-49yv65gm 117 48 . . . cord-279980-49yv65gm 118 1 RT RT NNP cord-279980-49yv65gm 118 2 - - HYPH cord-279980-49yv65gm 118 3 PCR PCR NNP cord-279980-49yv65gm 118 4 reactions reaction NNS cord-279980-49yv65gm 118 5 were be VBD cord-279980-49yv65gm 118 6 performed perform VBN cord-279980-49yv65gm 118 7 using use VBG cord-279980-49yv65gm 118 8 the the DT cord-279980-49yv65gm 118 9 AccessQuick AccessQuick NNP cord-279980-49yv65gm 118 10 ™ ™ HYPH cord-279980-49yv65gm 118 11 RT RT NNP cord-279980-49yv65gm 118 12 - - HYPH cord-279980-49yv65gm 118 13 PCR PCR NNP cord-279980-49yv65gm 118 14 system system NN cord-279980-49yv65gm 118 15 ( ( -LRB- cord-279980-49yv65gm 118 16 Promega Promega NNP cord-279980-49yv65gm 118 17 , , , cord-279980-49yv65gm 118 18 Madison Madison NNP cord-279980-49yv65gm 118 19 , , , cord-279980-49yv65gm 118 20 WI WI NNP cord-279980-49yv65gm 118 21 , , , cord-279980-49yv65gm 118 22 USA USA NNP cord-279980-49yv65gm 118 23 ) ) -RRB- cord-279980-49yv65gm 118 24 according accord VBG cord-279980-49yv65gm 118 25 to to IN cord-279980-49yv65gm 118 26 the the DT cord-279980-49yv65gm 118 27 manufacturer manufacturer NN cord-279980-49yv65gm 118 28 's 's POS cord-279980-49yv65gm 118 29 protocol protocol NN cord-279980-49yv65gm 118 30 . . . cord-279980-49yv65gm 119 1 RT RT NNP cord-279980-49yv65gm 119 2 - - HYPH cord-279980-49yv65gm 119 3 PCR PCR NNP cord-279980-49yv65gm 119 4 condition condition NN cord-279980-49yv65gm 119 5 consisted consist VBD cord-279980-49yv65gm 119 6 of of IN cord-279980-49yv65gm 119 7 a a DT cord-279980-49yv65gm 119 8 reverse reverse JJ cord-279980-49yv65gm 119 9 transcription transcription NN cord-279980-49yv65gm 119 10 step step NN cord-279980-49yv65gm 119 11 for for IN cord-279980-49yv65gm 119 12 15 15 CD cord-279980-49yv65gm 119 13 min min NN cord-279980-49yv65gm 119 14 at at IN cord-279980-49yv65gm 119 15 48 48 CD cord-279980-49yv65gm 119 16 • • NNP cord-279980-49yv65gm 119 17 C C NNP cord-279980-49yv65gm 119 18 , , , cord-279980-49yv65gm 119 19 an an DT cord-279980-49yv65gm 119 20 initial initial JJ cord-279980-49yv65gm 119 21 denaturation denaturation NN cord-279980-49yv65gm 119 22 step step NN cord-279980-49yv65gm 119 23 for for IN cord-279980-49yv65gm 119 24 2 2 CD cord-279980-49yv65gm 119 25 min min NN cord-279980-49yv65gm 119 26 at at IN cord-279980-49yv65gm 119 27 95 95 CD cord-279980-49yv65gm 119 28 • • NN cord-279980-49yv65gm 119 29 C c NN cord-279980-49yv65gm 119 30 , , , cord-279980-49yv65gm 119 31 an an DT cord-279980-49yv65gm 119 32 amplification amplification NN cord-279980-49yv65gm 119 33 step step NN cord-279980-49yv65gm 119 34 for for IN cord-279980-49yv65gm 119 35 40 40 CD cord-279980-49yv65gm 119 36 cycles cycle NNS cord-279980-49yv65gm 119 37 ( ( -LRB- cord-279980-49yv65gm 119 38 30 30 CD cord-279980-49yv65gm 119 39 s s NN cord-279980-49yv65gm 119 40 at at IN cord-279980-49yv65gm 119 41 94 94 CD cord-279980-49yv65gm 120 1 • • NNP cord-279980-49yv65gm 120 2 C C NNP cord-279980-49yv65gm 120 3 , , , cord-279980-49yv65gm 120 4 30 30 CD cord-279980-49yv65gm 120 5 s s NN cord-279980-49yv65gm 120 6 at at IN cord-279980-49yv65gm 120 7 55 55 CD cord-279980-49yv65gm 121 1 • • NNP cord-279980-49yv65gm 121 2 C C NNP cord-279980-49yv65gm 121 3 and and CC cord-279980-49yv65gm 121 4 30 30 CD cord-279980-49yv65gm 121 5 s s NN cord-279980-49yv65gm 121 6 at at IN cord-279980-49yv65gm 121 7 72 72 CD cord-279980-49yv65gm 121 8 • • NN cord-279980-49yv65gm 121 9 C C NNP cord-279980-49yv65gm 121 10 ) ) -RRB- cord-279980-49yv65gm 121 11 and and CC cord-279980-49yv65gm 121 12 a a DT cord-279980-49yv65gm 121 13 final final JJ cord-279980-49yv65gm 121 14 extension extension NN cord-279980-49yv65gm 121 15 step step NN cord-279980-49yv65gm 121 16 for for IN cord-279980-49yv65gm 121 17 10 10 CD cord-279980-49yv65gm 121 18 min min NN cord-279980-49yv65gm 121 19 at at IN cord-279980-49yv65gm 121 20 72 72 CD cord-279980-49yv65gm 122 1 • • NNP cord-279980-49yv65gm 122 2 C. C. NNP cord-279980-49yv65gm 123 1 The the DT cord-279980-49yv65gm 123 2 PCR PCR NNP cord-279980-49yv65gm 123 3 product product NN cord-279980-49yv65gm 123 4 was be VBD cord-279980-49yv65gm 123 5 analysed analyse VBN cord-279980-49yv65gm 123 6 by by IN cord-279980-49yv65gm 123 7 agarose agarose JJ cord-279980-49yv65gm 123 8 gel gel NN cord-279980-49yv65gm 123 9 electrophoresis electrophoresis NN cord-279980-49yv65gm 123 10 . . . cord-279980-49yv65gm 124 1 The the DT cord-279980-49yv65gm 124 2 expected expect VBN cord-279980-49yv65gm 124 3 PCR PCR NNP cord-279980-49yv65gm 124 4 product product NN cord-279980-49yv65gm 124 5 size size NN cord-279980-49yv65gm 124 6 of of IN cord-279980-49yv65gm 124 7 the the DT cord-279980-49yv65gm 124 8 M M NNP cord-279980-49yv65gm 124 9 gene gene NN cord-279980-49yv65gm 124 10 was be VBD cord-279980-49yv65gm 124 11 276 276 CD cord-279980-49yv65gm 124 12 bp bp NNP cord-279980-49yv65gm 124 13 . . . cord-279980-49yv65gm 125 1 The the DT cord-279980-49yv65gm 125 2 allantoic allantoic NNP cord-279980-49yv65gm 125 3 fluid fluid NN cord-279980-49yv65gm 125 4 from from IN cord-279980-49yv65gm 125 5 non non JJ cord-279980-49yv65gm 125 6 - - JJ cord-279980-49yv65gm 125 7 exposed exposed JJ cord-279980-49yv65gm 125 8 AI AI NNP cord-279980-49yv65gm 125 9 H5N1 H5N1 NNP cord-279980-49yv65gm 125 10 house house NN cord-279980-49yv65gm 125 11 flies fly VBZ cord-279980-49yv65gm 125 12 inoculated inoculate VBD cord-279980-49yv65gm 125 13 embryonated embryonated JJ cord-279980-49yv65gm 125 14 chicken chicken NN cord-279980-49yv65gm 125 15 eggs egg NNS cord-279980-49yv65gm 125 16 and and CC cord-279980-49yv65gm 125 17 the the DT cord-279980-49yv65gm 125 18 allantoic allantoic NNP cord-279980-49yv65gm 125 19 fluid fluid NN cord-279980-49yv65gm 125 20 of of IN cord-279980-49yv65gm 125 21 AI AI NNP cord-279980-49yv65gm 125 22 H5N1 H5N1 NNP cord-279980-49yv65gm 125 23 stock stock NN cord-279980-49yv65gm 125 24 virus virus NN cord-279980-49yv65gm 125 25 were be VBD cord-279980-49yv65gm 125 26 used use VBN cord-279980-49yv65gm 125 27 as as IN cord-279980-49yv65gm 125 28 the the DT cord-279980-49yv65gm 125 29 negative negative JJ cord-279980-49yv65gm 125 30 and and CC cord-279980-49yv65gm 125 31 positive positive JJ cord-279980-49yv65gm 125 32 controls control NNS cord-279980-49yv65gm 125 33 of of IN cord-279980-49yv65gm 125 34 this this DT cord-279980-49yv65gm 125 35 assay assay NN cord-279980-49yv65gm 125 36 , , , cord-279980-49yv65gm 125 37 respectively respectively RB cord-279980-49yv65gm 125 38 . . . cord-279980-49yv65gm 126 1 The the DT cord-279980-49yv65gm 126 2 RRT RRT NNP cord-279980-49yv65gm 126 3 - - HYPH cord-279980-49yv65gm 126 4 PCR pcr NN cord-279980-49yv65gm 126 5 assay assay NN cord-279980-49yv65gm 126 6 for for IN cord-279980-49yv65gm 126 7 M M NNP cord-279980-49yv65gm 126 8 gene gene NN cord-279980-49yv65gm 126 9 detection detection NN cord-279980-49yv65gm 126 10 , , , cord-279980-49yv65gm 126 11 modified modify VBN cord-279980-49yv65gm 126 12 from from IN cord-279980-49yv65gm 126 13 Spackman Spackman NNP cord-279980-49yv65gm 126 14 et et NNP cord-279980-49yv65gm 126 15 al al NNP cord-279980-49yv65gm 126 16 . . . cord-279980-49yv65gm 127 1 ( ( -LRB- cord-279980-49yv65gm 127 2 2002 2002 CD cord-279980-49yv65gm 127 3 ) ) -RRB- cord-279980-49yv65gm 127 4 , , , cord-279980-49yv65gm 127 5 was be VBD cord-279980-49yv65gm 127 6 performed perform VBN cord-279980-49yv65gm 127 7 using use VBG cord-279980-49yv65gm 127 8 the the DT cord-279980-49yv65gm 127 9 SuperScript SuperScript NNP cord-279980-49yv65gm 127 10 III III NNP cord-279980-49yv65gm 127 11 Platinum Platinum NNP cord-279980-49yv65gm 127 12 One one CD cord-279980-49yv65gm 127 13 - - HYPH cord-279980-49yv65gm 127 14 step step NN cord-279980-49yv65gm 127 15 RT RT NNP cord-279980-49yv65gm 127 16 - - HYPH cord-279980-49yv65gm 127 17 PCR PCR NNP cord-279980-49yv65gm 127 18 system system NN cord-279980-49yv65gm 127 19 ( ( -LRB- cord-279980-49yv65gm 127 20 Invitrogen Invitrogen NNP cord-279980-49yv65gm 127 21 , , , cord-279980-49yv65gm 127 22 Carlsbad Carlsbad NNP cord-279980-49yv65gm 127 23 , , , cord-279980-49yv65gm 127 24 CA CA NNP cord-279980-49yv65gm 127 25 , , , cord-279980-49yv65gm 127 26 USA USA NNP cord-279980-49yv65gm 127 27 ) ) -RRB- cord-279980-49yv65gm 127 28 . . . cord-279980-49yv65gm 128 1 The the DT cord-279980-49yv65gm 128 2 forward forward JJ cord-279980-49yv65gm 128 3 primer primer NN cord-279980-49yv65gm 128 4 MF25 MF25 NNP cord-279980-49yv65gm 128 5 ( ( -LRB- cord-279980-49yv65gm 128 6 5 5 CD cord-279980-49yv65gm 128 7 -AGATGA -agatga CD cord-279980-49yv65gm 128 8 GTCTTCTAACCGAGGTCG-3 GTCTTCTAACCGAGGTCG-3 NNP cord-279980-49yv65gm 128 9 ) ) -RRB- cord-279980-49yv65gm 128 10 , , , cord-279980-49yv65gm 128 11 reverse reverse VB cord-279980-49yv65gm 128 12 primer primer NN cord-279980-49yv65gm 128 13 MR124 mr124 NN cord-279980-49yv65gm 128 14 ( ( -LRB- cord-279980-49yv65gm 128 15 5 5 CD cord-279980-49yv65gm 128 16 -TGCAAAAACATCTTCAAGTCTCTG-3 -TGCAAAAACATCTTCAAGTCTCTG-3 NNP cord-279980-49yv65gm 128 17 ) ) -RRB- cord-279980-49yv65gm 128 18 and and CC cord-279980-49yv65gm 128 19 probe probe VB cord-279980-49yv65gm 128 20 M64 M64 NNP cord-279980-49yv65gm 128 21 ( ( -LRB- cord-279980-49yv65gm 128 22 FAM FAM NNP cord-279980-49yv65gm 128 23 - - HYPH cord-279980-49yv65gm 128 24 TCAGGCCCCCTCAAAGCCGA TCAGGCCCCCTCAAAGCCGA NNP cord-279980-49yv65gm 128 25 - - HYPH cord-279980-49yv65gm 128 26 TAMRA TAMRA NNP cord-279980-49yv65gm 128 27 ) ) -RRB- cord-279980-49yv65gm 128 28 were be VBD cord-279980-49yv65gm 128 29 used use VBN cord-279980-49yv65gm 128 30 in in IN cord-279980-49yv65gm 128 31 the the DT cord-279980-49yv65gm 128 32 present present JJ cord-279980-49yv65gm 128 33 study study NN cord-279980-49yv65gm 128 34 ( ( -LRB- cord-279980-49yv65gm 128 35 Spackman Spackman NNP cord-279980-49yv65gm 128 36 et et NNP cord-279980-49yv65gm 128 37 al al NNP cord-279980-49yv65gm 128 38 . . NNP cord-279980-49yv65gm 128 39 , , , cord-279980-49yv65gm 128 40 2002 2002 CD cord-279980-49yv65gm 128 41 ) ) -RRB- cord-279980-49yv65gm 128 42 . . . cord-279980-49yv65gm 129 1 Final final JJ cord-279980-49yv65gm 129 2 concentrations concentration NNS cord-279980-49yv65gm 129 3 of of IN cord-279980-49yv65gm 129 4 primers primer NNS cord-279980-49yv65gm 129 5 and and CC cord-279980-49yv65gm 129 6 probe probe NN cord-279980-49yv65gm 129 7 were be VBD cord-279980-49yv65gm 129 8 10 10 CD cord-279980-49yv65gm 129 9 and and CC cord-279980-49yv65gm 129 10 2.5 2.5 CD cord-279980-49yv65gm 129 11 μm μm NNS cord-279980-49yv65gm 129 12 , , , cord-279980-49yv65gm 129 13 respectively respectively RB cord-279980-49yv65gm 129 14 . . . cord-279980-49yv65gm 130 1 The the DT cord-279980-49yv65gm 130 2 reaction reaction NN cord-279980-49yv65gm 130 3 consisted consist VBD cord-279980-49yv65gm 130 4 of of IN cord-279980-49yv65gm 130 5 4 4 CD cord-279980-49yv65gm 130 6 μL μL NNS cord-279980-49yv65gm 130 7 of of IN cord-279980-49yv65gm 130 8 the the DT cord-279980-49yv65gm 130 9 RNA RNA NNP cord-279980-49yv65gm 130 10 sample sample NN cord-279980-49yv65gm 130 11 , , , cord-279980-49yv65gm 130 12 7.5 7.5 CD cord-279980-49yv65gm 130 13 μL μl NN cord-279980-49yv65gm 130 14 of of IN cord-279980-49yv65gm 130 15 2× 2× CD cord-279980-49yv65gm 130 16 Reaction Reaction NNP cord-279980-49yv65gm 130 17 Mix Mix NNP cord-279980-49yv65gm 130 18 , , , cord-279980-49yv65gm 130 19 0.3 0.3 CD cord-279980-49yv65gm 130 20 μL μl NN cord-279980-49yv65gm 130 21 of of IN cord-279980-49yv65gm 130 22 SuperScript SuperScript NNP cord-279980-49yv65gm 130 23 III III NNP cord-279980-49yv65gm 131 1 RT RT NNP cord-279980-49yv65gm 131 2 Platinum Platinum NNP cord-279980-49yv65gm 131 3 ® ® . cord-279980-49yv65gm 131 4 Taq Taq NNP cord-279980-49yv65gm 131 5 Mix Mix NNP cord-279980-49yv65gm 131 6 , , , cord-279980-49yv65gm 131 7 50 50 CD cord-279980-49yv65gm 131 8 mM mm CD cord-279980-49yv65gm 131 9 of of IN cord-279980-49yv65gm 131 10 MgSO MgSO NNP cord-279980-49yv65gm 131 11 4 4 CD cord-279980-49yv65gm 131 12 and and CC cord-279980-49yv65gm 131 13 RNase rnase NN cord-279980-49yv65gm 131 14 - - HYPH cord-279980-49yv65gm 131 15 free free JJ cord-279980-49yv65gm 131 16 water water NN cord-279980-49yv65gm 131 17 in in IN cord-279980-49yv65gm 131 18 a a DT cord-279980-49yv65gm 131 19 final final JJ cord-279980-49yv65gm 131 20 volume volume NN cord-279980-49yv65gm 131 21 of of IN cord-279980-49yv65gm 131 22 17 17 CD cord-279980-49yv65gm 131 23 μL. μl. JJ cord-279980-49yv65gm 131 24 One one CD cord-279980-49yv65gm 131 25 - - HYPH cord-279980-49yv65gm 131 26 step step NN cord-279980-49yv65gm 131 27 RRT RRT NNP cord-279980-49yv65gm 131 28 - - HYPH cord-279980-49yv65gm 131 29 PCR PCR NNP cord-279980-49yv65gm 131 30 was be VBD cord-279980-49yv65gm 131 31 performed perform VBN cord-279980-49yv65gm 131 32 using use VBG cord-279980-49yv65gm 131 33 Rotor rotor NN cord-279980-49yv65gm 131 34 - - HYPH cord-279980-49yv65gm 131 35 Gene gene NN cord-279980-49yv65gm 131 36 3000 3000 CD cord-279980-49yv65gm 131 37 ( ( -LRB- cord-279980-49yv65gm 131 38 Corbett Corbett NNP cord-279980-49yv65gm 131 39 Research Research NNP cord-279980-49yv65gm 131 40 , , , cord-279980-49yv65gm 131 41 New New NNP cord-279980-49yv65gm 131 42 South South NNP cord-279980-49yv65gm 131 43 Wales Wales NNP cord-279980-49yv65gm 131 44 , , , cord-279980-49yv65gm 131 45 Australia Australia NNP cord-279980-49yv65gm 131 46 ) ) -RRB- cord-279980-49yv65gm 131 47 . . . cord-279980-49yv65gm 132 1 Cycling cycling NN cord-279980-49yv65gm 132 2 conditions condition NNS cord-279980-49yv65gm 132 3 included include VBD cord-279980-49yv65gm 132 4 a a DT cord-279980-49yv65gm 132 5 reverse reverse JJ cord-279980-49yv65gm 132 6 transcription transcription NN cord-279980-49yv65gm 132 7 step step NN cord-279980-49yv65gm 132 8 at at IN cord-279980-49yv65gm 132 9 50 50 CD cord-279980-49yv65gm 132 10 • • NNS cord-279980-49yv65gm 132 11 C c NN cord-279980-49yv65gm 132 12 30 30 CD cord-279980-49yv65gm 132 13 min min NN cord-279980-49yv65gm 132 14 , , , cord-279980-49yv65gm 132 15 initial initial JJ cord-279980-49yv65gm 132 16 denaturation denaturation NN cord-279980-49yv65gm 132 17 step step NN cord-279980-49yv65gm 132 18 at at IN cord-279980-49yv65gm 132 19 94 94 CD cord-279980-49yv65gm 133 1 • • NNP cord-279980-49yv65gm 134 1 C C NNP cord-279980-49yv65gm 134 2 15 15 CD cord-279980-49yv65gm 134 3 min min NN cord-279980-49yv65gm 134 4 followed follow VBN cord-279980-49yv65gm 134 5 by by IN cord-279980-49yv65gm 134 6 40 40 CD cord-279980-49yv65gm 134 7 cycles cycle NNS cord-279980-49yv65gm 134 8 of of IN cord-279980-49yv65gm 134 9 amplification amplification NN cord-279980-49yv65gm 134 10 ( ( -LRB- cord-279980-49yv65gm 134 11 95 95 CD cord-279980-49yv65gm 134 12 • • NNS cord-279980-49yv65gm 135 1 C C NNP cord-279980-49yv65gm 135 2 for for IN cord-279980-49yv65gm 135 3 10 10 CD cord-279980-49yv65gm 135 4 s s NN cord-279980-49yv65gm 136 1 , , , cord-279980-49yv65gm 136 2 54 54 CD cord-279980-49yv65gm 136 3 • • NN cord-279980-49yv65gm 136 4 C c NN cord-279980-49yv65gm 136 5 for for IN cord-279980-49yv65gm 136 6 30 30 CD cord-279980-49yv65gm 136 7 s s NN cord-279980-49yv65gm 136 8 and and CC cord-279980-49yv65gm 136 9 72 72 CD cord-279980-49yv65gm 137 1 • • NNP cord-279980-49yv65gm 138 1 C C NNP cord-279980-49yv65gm 138 2 for for IN cord-279980-49yv65gm 138 3 10 10 CD cord-279980-49yv65gm 138 4 s s NNS cord-279980-49yv65gm 138 5 ) ) -RRB- cord-279980-49yv65gm 139 1 ( ( -LRB- cord-279980-49yv65gm 139 2 Spackman Spackman NNP cord-279980-49yv65gm 139 3 et et NNP cord-279980-49yv65gm 139 4 al al NNP cord-279980-49yv65gm 139 5 . . NNP cord-279980-49yv65gm 139 6 , , , cord-279980-49yv65gm 139 7 2002 2002 CD cord-279980-49yv65gm 139 8 ) ) -RRB- cord-279980-49yv65gm 139 9 . . . cord-279980-49yv65gm 140 1 The the DT cord-279980-49yv65gm 140 2 fluorescence fluorescence NN cord-279980-49yv65gm 140 3 data datum NNS cord-279980-49yv65gm 140 4 were be VBD cord-279980-49yv65gm 140 5 collected collect VBN cord-279980-49yv65gm 140 6 at at IN cord-279980-49yv65gm 140 7 the the DT cord-279980-49yv65gm 140 8 end end NN cord-279980-49yv65gm 140 9 of of IN cord-279980-49yv65gm 140 10 each each DT cord-279980-49yv65gm 140 11 annealing anneal VBG cord-279980-49yv65gm 140 12 step step NN cord-279980-49yv65gm 140 13 and and CC cord-279980-49yv65gm 140 14 the the DT cord-279980-49yv65gm 140 15 data data NN cord-279980-49yv65gm 140 16 analyses analysis NNS cord-279980-49yv65gm 140 17 of of IN cord-279980-49yv65gm 140 18 the the DT cord-279980-49yv65gm 140 19 RRT RRT NNP cord-279980-49yv65gm 140 20 - - HYPH cord-279980-49yv65gm 140 21 PCR PCR NNP cord-279980-49yv65gm 140 22 assay assay NN cord-279980-49yv65gm 140 23 was be VBD cord-279980-49yv65gm 140 24 performed perform VBN cord-279980-49yv65gm 140 25 using use VBG cord-279980-49yv65gm 140 26 the the DT cord-279980-49yv65gm 140 27 Rotor Rotor NNP cord-279980-49yv65gm 140 28 - - HYPH cord-279980-49yv65gm 140 29 Gene gene NN cord-279980-49yv65gm 140 30 analysis analysis NN cord-279980-49yv65gm 140 31 software software NN cord-279980-49yv65gm 140 32 , , , cord-279980-49yv65gm 140 33 Version version NN cord-279980-49yv65gm 140 34 6.0 6.0 CD cord-279980-49yv65gm 140 35 ( ( -LRB- cord-279980-49yv65gm 140 36 Corbett Corbett NNP cord-279980-49yv65gm 140 37 research research NN cord-279980-49yv65gm 140 38 supporting support VBG cord-279980-49yv65gm 140 39 program program NN cord-279980-49yv65gm 140 40 , , , cord-279980-49yv65gm 140 41 Corbett Corbett NNP cord-279980-49yv65gm 140 42 Research Research NNP cord-279980-49yv65gm 140 43 , , , cord-279980-49yv65gm 140 44 Sydney Sydney NNP cord-279980-49yv65gm 140 45 , , , cord-279980-49yv65gm 140 46 Australia Australia NNP cord-279980-49yv65gm 140 47 ) ) -RRB- cord-279980-49yv65gm 140 48 . . . cord-279980-49yv65gm 141 1 The the DT cord-279980-49yv65gm 141 2 negative negative JJ cord-279980-49yv65gm 141 3 and and CC cord-279980-49yv65gm 141 4 positive positive JJ cord-279980-49yv65gm 141 5 controls control NNS cord-279980-49yv65gm 141 6 of of IN cord-279980-49yv65gm 141 7 this this DT cord-279980-49yv65gm 141 8 assay assay NN cord-279980-49yv65gm 141 9 were be VBD cord-279980-49yv65gm 141 10 similar similar JJ cord-279980-49yv65gm 141 11 to to IN cord-279980-49yv65gm 141 12 the the DT cord-279980-49yv65gm 141 13 RT RT NNP cord-279980-49yv65gm 141 14 - - HYPH cord-279980-49yv65gm 141 15 PCR PCR NNP cord-279980-49yv65gm 141 16 assay assay NN cord-279980-49yv65gm 141 17 . . . cord-279980-49yv65gm 142 1 Student student NN cord-279980-49yv65gm 142 2 's 's POS cord-279980-49yv65gm 142 3 paired paired JJ cord-279980-49yv65gm 142 4 t t NN cord-279980-49yv65gm 142 5 - - HYPH cord-279980-49yv65gm 142 6 test test NN cord-279980-49yv65gm 142 7 was be VBD cord-279980-49yv65gm 142 8 used use VBN cord-279980-49yv65gm 142 9 in in IN cord-279980-49yv65gm 142 10 experiment experiment NN cord-279980-49yv65gm 142 11 2 2 CD cord-279980-49yv65gm 142 12 to to TO cord-279980-49yv65gm 142 13 compare compare VB cord-279980-49yv65gm 142 14 the the DT cord-279980-49yv65gm 142 15 virus virus NN cord-279980-49yv65gm 142 16 titre titre NN cord-279980-49yv65gm 142 17 between between IN cord-279980-49yv65gm 142 18 a a DT cord-279980-49yv65gm 142 19 house house NN cord-279980-49yv65gm 142 20 fly fly VB cord-279980-49yv65gm 142 21 homogenate homogenate NNP cord-279980-49yv65gm 142 22 and and CC cord-279980-49yv65gm 142 23 W1 W1 NNP cord-279980-49yv65gm 142 24 washing washing NN cord-279980-49yv65gm 142 25 fluid fluid NN cord-279980-49yv65gm 142 26 . . . cord-279980-49yv65gm 143 1 In in IN cord-279980-49yv65gm 143 2 experiment experiment NN cord-279980-49yv65gm 143 3 3 3 CD cord-279980-49yv65gm 143 4 , , , cord-279980-49yv65gm 143 5 anova anova NNP cord-279980-49yv65gm 143 6 with with IN cord-279980-49yv65gm 143 7 a a DT cord-279980-49yv65gm 143 8 Tukey Tukey NNP cord-279980-49yv65gm 143 9 - - HYPH cord-279980-49yv65gm 143 10 Kramer Kramer NNP cord-279980-49yv65gm 143 11 multiple multiple JJ cord-279980-49yv65gm 143 12 comparison comparison NN cord-279980-49yv65gm 143 13 test test NN cord-279980-49yv65gm 143 14 was be VBD cord-279980-49yv65gm 143 15 used use VBN cord-279980-49yv65gm 143 16 to to TO cord-279980-49yv65gm 143 17 determine determine VB cord-279980-49yv65gm 143 18 the the DT cord-279980-49yv65gm 143 19 correlation correlation NN cord-279980-49yv65gm 143 20 among among IN cord-279980-49yv65gm 143 21 the the DT cord-279980-49yv65gm 143 22 repeated repeat VBN cord-279980-49yv65gm 143 23 measures measure NNS cord-279980-49yv65gm 143 24 . . . cord-279980-49yv65gm 144 1 A a DT cord-279980-49yv65gm 144 2 P p NN cord-279980-49yv65gm 144 3 -value -value : cord-279980-49yv65gm 144 4 was be VBD cord-279980-49yv65gm 144 5 considered consider VBN cord-279980-49yv65gm 144 6 as as IN cord-279980-49yv65gm 144 7 a a DT cord-279980-49yv65gm 144 8 significant significant JJ cord-279980-49yv65gm 144 9 difference difference NN cord-279980-49yv65gm 144 10 ( ( -LRB- cord-279980-49yv65gm 144 11 P p NN cord-279980-49yv65gm 144 12 < < XX cord-279980-49yv65gm 144 13 0.05 0.05 CD cord-279980-49yv65gm 144 14 ) ) -RRB- cord-279980-49yv65gm 144 15 for for IN cord-279980-49yv65gm 144 16 all all DT cord-279980-49yv65gm 144 17 tests test NNS cord-279980-49yv65gm 144 18 . . . cord-279980-49yv65gm 145 1 In in IN cord-279980-49yv65gm 145 2 addition addition NN cord-279980-49yv65gm 145 3 , , , cord-279980-49yv65gm 145 4 the the DT cord-279980-49yv65gm 145 5 linear linear JJ cord-279980-49yv65gm 145 6 regression regression NN cord-279980-49yv65gm 145 7 analysis analysis NN cord-279980-49yv65gm 145 8 was be VBD cord-279980-49yv65gm 145 9 used use VBN cord-279980-49yv65gm 145 10 to to TO cord-279980-49yv65gm 145 11 evaluate evaluate VB cord-279980-49yv65gm 145 12 the the DT cord-279980-49yv65gm 145 13 correlation correlation NN cord-279980-49yv65gm 145 14 between between IN cord-279980-49yv65gm 145 15 virus virus NN cord-279980-49yv65gm 145 16 titre titre NN cord-279980-49yv65gm 145 17 and and CC cord-279980-49yv65gm 145 18 the the DT cord-279980-49yv65gm 145 19 time time NN cord-279980-49yv65gm 145 20 of of IN cord-279980-49yv65gm 145 21 post post JJ cord-279980-49yv65gm 145 22 - - JJ cord-279980-49yv65gm 145 23 exposure exposure NN cord-279980-49yv65gm 145 24 . . . cord-279980-49yv65gm 146 1 Embryonated embryonate VBN cord-279980-49yv65gm 146 2 chicken chicken NN cord-279980-49yv65gm 146 3 eggs egg NNS cord-279980-49yv65gm 146 4 inoculated inoculate VBN cord-279980-49yv65gm 146 5 with with IN cord-279980-49yv65gm 146 6 W1 w1 NN cord-279980-49yv65gm 146 7 washing washing NN cord-279980-49yv65gm 146 8 fluid fluid NN cord-279980-49yv65gm 146 9 from from IN cord-279980-49yv65gm 146 10 treatment treatment NN cord-279980-49yv65gm 146 11 groups group NNS cord-279980-49yv65gm 146 12 and and CC cord-279980-49yv65gm 146 13 house house NN cord-279980-49yv65gm 146 14 fly fly VB cord-279980-49yv65gm 146 15 homogenates homogenate NNS cord-279980-49yv65gm 146 16 from from IN cord-279980-49yv65gm 146 17 the the DT cord-279980-49yv65gm 146 18 treatment treatment NN cord-279980-49yv65gm 146 19 group group NN cord-279980-49yv65gm 147 1 died die VBD cord-279980-49yv65gm 147 2 within within IN cord-279980-49yv65gm 147 3 2 2 CD cord-279980-49yv65gm 147 4 days day NNS cord-279980-49yv65gm 147 5 post post NN cord-279980-49yv65gm 147 6 - - NN cord-279980-49yv65gm 147 7 inoculation inoculation NN cord-279980-49yv65gm 147 8 . . . cord-279980-49yv65gm 148 1 Allantoic Allantoic NNP cord-279980-49yv65gm 148 2 fluid fluid NN cord-279980-49yv65gm 148 3 from from IN cord-279980-49yv65gm 148 4 the the DT cord-279980-49yv65gm 148 5 treatment treatment NN cord-279980-49yv65gm 148 6 and and CC cord-279980-49yv65gm 148 7 W1 w1 NN cord-279980-49yv65gm 148 8 washing washing NN cord-279980-49yv65gm 148 9 fluid fluid NN cord-279980-49yv65gm 148 10 were be VBD cord-279980-49yv65gm 148 11 positive positive JJ cord-279980-49yv65gm 148 12 for for IN cord-279980-49yv65gm 148 13 the the DT cord-279980-49yv65gm 148 14 AI AI NNP cord-279980-49yv65gm 148 15 H5N1 H5N1 NNP cord-279980-49yv65gm 148 16 virus virus NN cord-279980-49yv65gm 148 17 using use VBG cord-279980-49yv65gm 148 18 the the DT cord-279980-49yv65gm 148 19 HA HA NNP cord-279980-49yv65gm 148 20 test test NN cord-279980-49yv65gm 148 21 and and CC cord-279980-49yv65gm 148 22 RT RT NNP cord-279980-49yv65gm 148 23 - - HYPH cord-279980-49yv65gm 148 24 PCR PCR NNP cord-279980-49yv65gm 148 25 assay assay NN cord-279980-49yv65gm 148 26 . . . cord-279980-49yv65gm 149 1 All all DT cord-279980-49yv65gm 149 2 embryonated embryonate VBN cord-279980-49yv65gm 149 3 chicken chicken NN cord-279980-49yv65gm 149 4 eggs egg NNS cord-279980-49yv65gm 149 5 inoculated inoculate VBN cord-279980-49yv65gm 149 6 with with IN cord-279980-49yv65gm 149 7 the the DT cord-279980-49yv65gm 149 8 W2 W2 NNP cord-279980-49yv65gm 149 9 washing washing NN cord-279980-49yv65gm 149 10 fluid fluid NN cord-279980-49yv65gm 149 11 ( ( -LRB- cord-279980-49yv65gm 149 12 after after IN cord-279980-49yv65gm 149 13 surface surface NN cord-279980-49yv65gm 149 14 sterilization sterilization NN cord-279980-49yv65gm 149 15 ) ) -RRB- cord-279980-49yv65gm 149 16 and and CC cord-279980-49yv65gm 149 17 negative negative JJ cord-279980-49yv65gm 149 18 control control NN cord-279980-49yv65gm 149 19 group group NN cord-279980-49yv65gm 149 20 remained remain VBD cord-279980-49yv65gm 149 21 healthy healthy JJ cord-279980-49yv65gm 149 22 with with IN cord-279980-49yv65gm 149 23 no no DT cord-279980-49yv65gm 149 24 evidence evidence NN cord-279980-49yv65gm 149 25 of of IN cord-279980-49yv65gm 149 26 AI AI NNP cord-279980-49yv65gm 149 27 H5N1 H5N1 NNP cord-279980-49yv65gm 149 28 virus virus NN cord-279980-49yv65gm 149 29 infection infection NN cord-279980-49yv65gm 149 30 . . . cord-279980-49yv65gm 150 1 The the DT cord-279980-49yv65gm 150 2 AI AI NNP cord-279980-49yv65gm 150 3 H5N1 H5N1 NNP cord-279980-49yv65gm 150 4 virus virus NN cord-279980-49yv65gm 150 5 was be VBD cord-279980-49yv65gm 150 6 detected detect VBN cord-279980-49yv65gm 150 7 in in IN cord-279980-49yv65gm 150 8 a a DT cord-279980-49yv65gm 150 9 single single JJ cord-279980-49yv65gm 150 10 house house NN cord-279980-49yv65gm 150 11 fly fly VB cord-279980-49yv65gm 150 12 in in IN cord-279980-49yv65gm 150 13 all all DT cord-279980-49yv65gm 150 14 replicates replicate NNS cord-279980-49yv65gm 150 15 . . . cord-279980-49yv65gm 151 1 Thirty thirty CD cord-279980-49yv65gm 151 2 embryonated embryonate VBN cord-279980-49yv65gm 151 3 chicken chicken NN cord-279980-49yv65gm 151 4 eggs egg NNS cord-279980-49yv65gm 151 5 from from IN cord-279980-49yv65gm 151 6 all all DT cord-279980-49yv65gm 151 7 replicates replicate NNS cord-279980-49yv65gm 151 8 of of IN cord-279980-49yv65gm 151 9 treatment treatment NN cord-279980-49yv65gm 151 10 groups group NNS cord-279980-49yv65gm 151 11 died die VBD cord-279980-49yv65gm 151 12 2 2 CD cord-279980-49yv65gm 151 13 days day NNS cord-279980-49yv65gm 151 14 post post NN cord-279980-49yv65gm 151 15 - - NN cord-279980-49yv65gm 151 16 inoculation inoculation NN cord-279980-49yv65gm 151 17 . . . cord-279980-49yv65gm 152 1 The the DT cord-279980-49yv65gm 152 2 average average JJ cord-279980-49yv65gm 152 3 virus virus NN cord-279980-49yv65gm 152 4 titre titre NN cord-279980-49yv65gm 152 5 of of IN cord-279980-49yv65gm 152 6 the the DT cord-279980-49yv65gm 152 7 house house NNP cord-279980-49yv65gm 152 8 fly fly NN cord-279980-49yv65gm 152 9 homogenate homogenate NNP cord-279980-49yv65gm 152 10 was be VBD cord-279980-49yv65gm 152 11 5.43 5.43 CD cord-279980-49yv65gm 152 12 ± ± CD cord-279980-49yv65gm 152 13 0.33 0.33 CD cord-279980-49yv65gm 152 14 log log NN cord-279980-49yv65gm 152 15 ELD ELD NNP cord-279980-49yv65gm 152 16 50 50 CD cord-279980-49yv65gm 152 17 /mL /mL NFP cord-279980-49yv65gm 152 18 , , , cord-279980-49yv65gm 152 19 which which WDT cord-279980-49yv65gm 152 20 was be VBD cord-279980-49yv65gm 152 21 significantly significantly RB cord-279980-49yv65gm 152 22 ( ( -LRB- cord-279980-49yv65gm 152 23 P p NN cord-279980-49yv65gm 152 24 < < XX cord-279980-49yv65gm 152 25 0.05 0.05 CD cord-279980-49yv65gm 152 26 ) ) -RRB- cord-279980-49yv65gm 152 27 higher high JJR cord-279980-49yv65gm 152 28 than than IN cord-279980-49yv65gm 152 29 that that DT cord-279980-49yv65gm 152 30 of of IN cord-279980-49yv65gm 152 31 1 1 CD cord-279980-49yv65gm 152 32 mL ml NN cord-279980-49yv65gm 152 33 of of IN cord-279980-49yv65gm 152 34 W1 w1 NN cord-279980-49yv65gm 152 35 washing washing NN cord-279980-49yv65gm 152 36 fluid fluid NN cord-279980-49yv65gm 152 37 ( ( -LRB- cord-279980-49yv65gm 152 38 external external JJ cord-279980-49yv65gm 152 39 surface surface NN cord-279980-49yv65gm 152 40 , , , cord-279980-49yv65gm 152 41 4.28 4.28 CD cord-279980-49yv65gm 152 42 ± ± CD cord-279980-49yv65gm 152 43 0.55 0.55 CD cord-279980-49yv65gm 152 44 log log NN cord-279980-49yv65gm 152 45 ELD ELD NNP cord-279980-49yv65gm 152 46 50 50 CD cord-279980-49yv65gm 152 47 /mL /mL . cord-279980-49yv65gm 152 48 ) ) -RRB- cord-279980-49yv65gm 152 49 . . . cord-279980-49yv65gm 153 1 The the DT cord-279980-49yv65gm 153 2 virus virus NN cord-279980-49yv65gm 153 3 titres titre NNS cord-279980-49yv65gm 153 4 between between IN cord-279980-49yv65gm 153 5 a a DT cord-279980-49yv65gm 153 6 house house NN cord-279980-49yv65gm 153 7 fly fly NN cord-279980-49yv65gm 153 8 homogenate homogenate NNP cord-279980-49yv65gm 153 9 and and CC cord-279980-49yv65gm 153 10 W1 W1 NNP cord-279980-49yv65gm 153 11 at at IN cord-279980-49yv65gm 153 12 different different JJ cord-279980-49yv65gm 153 13 times time NNS cord-279980-49yv65gm 153 14 decreased decrease VBD cord-279980-49yv65gm 153 15 after after IN cord-279980-49yv65gm 153 16 48 48 CD cord-279980-49yv65gm 153 17 and and CC cord-279980-49yv65gm 153 18 24 24 CD cord-279980-49yv65gm 153 19 h h NN cord-279980-49yv65gm 153 20 , , , cord-279980-49yv65gm 153 21 respectively respectively RB cord-279980-49yv65gm 153 22 ( ( -LRB- cord-279980-49yv65gm 153 23 Table table NN cord-279980-49yv65gm 153 24 1 1 CD cord-279980-49yv65gm 153 25 ) ) -RRB- cord-279980-49yv65gm 153 26 . . . cord-279980-49yv65gm 154 1 The the DT cord-279980-49yv65gm 154 2 HA HA NNP cord-279980-49yv65gm 154 3 test test NN cord-279980-49yv65gm 154 4 and and CC cord-279980-49yv65gm 154 5 RT RT NNP cord-279980-49yv65gm 154 6 - - HYPH cord-279980-49yv65gm 154 7 PCR PCR NNP cord-279980-49yv65gm 154 8 results result NNS cord-279980-49yv65gm 154 9 were be VBD cord-279980-49yv65gm 154 10 also also RB cord-279980-49yv65gm 154 11 positive positive JJ cord-279980-49yv65gm 154 12 in in IN cord-279980-49yv65gm 154 13 all all DT cord-279980-49yv65gm 154 14 groups group NNS cord-279980-49yv65gm 154 15 and and CC cord-279980-49yv65gm 154 16 in in IN cord-279980-49yv65gm 154 17 all all DT cord-279980-49yv65gm 154 18 replicates replicate NNS cord-279980-49yv65gm 154 19 . . . cord-279980-49yv65gm 155 1 In in IN cord-279980-49yv65gm 155 2 the the DT cord-279980-49yv65gm 155 3 negative negative JJ cord-279980-49yv65gm 155 4 control control NN cord-279980-49yv65gm 155 5 group group NN cord-279980-49yv65gm 155 6 , , , cord-279980-49yv65gm 155 7 all all DT cord-279980-49yv65gm 155 8 embryonated embryonate VBN cord-279980-49yv65gm 155 9 chicken chicken NN cord-279980-49yv65gm 155 10 eggs egg NNS cord-279980-49yv65gm 155 11 were be VBD cord-279980-49yv65gm 155 12 alive alive JJ cord-279980-49yv65gm 155 13 and and CC cord-279980-49yv65gm 155 14 the the DT cord-279980-49yv65gm 155 15 RT RT NNP cord-279980-49yv65gm 155 16 - - HYPH cord-279980-49yv65gm 155 17 PCR PCR NNP cord-279980-49yv65gm 155 18 results result NNS cord-279980-49yv65gm 155 19 were be VBD cord-279980-49yv65gm 155 20 negative negative JJ cord-279980-49yv65gm 155 21 . . . cord-279980-49yv65gm 156 1 The the DT cord-279980-49yv65gm 156 2 results result NNS cord-279980-49yv65gm 156 3 of of IN cord-279980-49yv65gm 156 4 the the DT cord-279980-49yv65gm 156 5 virus virus NN cord-279980-49yv65gm 156 6 isolation isolation NN cord-279980-49yv65gm 156 7 revealed reveal VBD cord-279980-49yv65gm 156 8 that that IN cord-279980-49yv65gm 156 9 the the DT cord-279980-49yv65gm 156 10 H5N1 H5N1 NNP cord-279980-49yv65gm 156 11 virus virus NN cord-279980-49yv65gm 156 12 could could MD cord-279980-49yv65gm 156 13 survive survive VB cord-279980-49yv65gm 156 14 in in IN cord-279980-49yv65gm 156 15 house house NN cord-279980-49yv65gm 156 16 flies fly NNS cord-279980-49yv65gm 156 17 and and CC cord-279980-49yv65gm 156 18 remained remain VBD cord-279980-49yv65gm 156 19 infective infective JJ cord-279980-49yv65gm 156 20 up up IN cord-279980-49yv65gm 156 21 to to TO cord-279980-49yv65gm 156 22 72 72 CD cord-279980-49yv65gm 156 23 h h NN cord-279980-49yv65gm 156 24 post post NN cord-279980-49yv65gm 156 25 - - NN cord-279980-49yv65gm 156 26 exposure exposure JJ cord-279980-49yv65gm 156 27 ( ( -LRB- cord-279980-49yv65gm 156 28 Fig fig NN cord-279980-49yv65gm 156 29 . . . cord-279980-49yv65gm 156 30 1 1 CD cord-279980-49yv65gm 156 31 ) ) -RRB- cord-279980-49yv65gm 156 32 . . . cord-279980-49yv65gm 157 1 Whereas whereas IN cord-279980-49yv65gm 157 2 , , , cord-279980-49yv65gm 157 3 the the DT cord-279980-49yv65gm 157 4 results result NNS cord-279980-49yv65gm 157 5 of of IN cord-279980-49yv65gm 157 6 the the DT cord-279980-49yv65gm 157 7 RRT RRT NNP cord-279980-49yv65gm 157 8 - - HYPH cord-279980-49yv65gm 157 9 PCR PCR NNP cord-279980-49yv65gm 157 10 assay assay NN cord-279980-49yv65gm 157 11 were be VBD cord-279980-49yv65gm 157 12 positive positive JJ cord-279980-49yv65gm 157 13 up up IN cord-279980-49yv65gm 157 14 to to IN cord-279980-49yv65gm 157 15 96 96 CD cord-279980-49yv65gm 157 16 h h NN cord-279980-49yv65gm 157 17 post post NN cord-279980-49yv65gm 157 18 - - NN cord-279980-49yv65gm 157 19 exposure exposure JJ cord-279980-49yv65gm 157 20 ( ( -LRB- cord-279980-49yv65gm 157 21 Table table NN cord-279980-49yv65gm 157 22 2 2 CD cord-279980-49yv65gm 157 23 ) ) -RRB- cord-279980-49yv65gm 157 24 , , , cord-279980-49yv65gm 157 25 indicating indicate VBG cord-279980-49yv65gm 157 26 that that IN cord-279980-49yv65gm 157 27 all all DT cord-279980-49yv65gm 157 28 house house NN cord-279980-49yv65gm 157 29 flies fly NNS cord-279980-49yv65gm 157 30 carried carry VBD cord-279980-49yv65gm 157 31 the the DT cord-279980-49yv65gm 157 32 AI AI NNP cord-279980-49yv65gm 157 33 H5N1 H5N1 NNP cord-279980-49yv65gm 157 34 virus virus NN cord-279980-49yv65gm 157 35 but but CC cord-279980-49yv65gm 157 36 Table table NN cord-279980-49yv65gm 157 37 1 1 CD cord-279980-49yv65gm 157 38 . . . cord-279980-49yv65gm 158 1 Avian avian JJ cord-279980-49yv65gm 158 2 influenza influenza NN cord-279980-49yv65gm 158 3 ( ( -LRB- cord-279980-49yv65gm 158 4 AI ai NN cord-279980-49yv65gm 158 5 ) ) -RRB- cord-279980-49yv65gm 159 1 H5N1 H5N1 NNP cord-279980-49yv65gm 159 2 virus virus NN cord-279980-49yv65gm 159 3 titre titre NN cord-279980-49yv65gm 159 4 between between IN cord-279980-49yv65gm 159 5 house house NNP cord-279980-49yv65gm 159 6 fly fly VB cord-279980-49yv65gm 159 7 homogenates homogenate NNS cord-279980-49yv65gm 159 8 and and CC cord-279980-49yv65gm 159 9 W1 w1 NN cord-279980-49yv65gm 159 10 of of IN cord-279980-49yv65gm 159 11 the the DT cord-279980-49yv65gm 159 12 AI AI NNP cord-279980-49yv65gm 159 13 H5N1 H5N1 NNP cord-279980-49yv65gm 159 14 virus virus NN cord-279980-49yv65gm 159 15 exposed expose VBD cord-279980-49yv65gm 159 16 house house NN cord-279980-49yv65gm 159 17 flies fly VBZ cord-279980-49yv65gm 159 18 at at IN cord-279980-49yv65gm 159 19 different different JJ cord-279980-49yv65gm 159 20 times time NNS cord-279980-49yv65gm 159 21 of of IN cord-279980-49yv65gm 159 22 post post JJ cord-279980-49yv65gm 159 23 - - JJ cord-279980-49yv65gm 159 24 exposure exposure NN cord-279980-49yv65gm 159 25 in in IN cord-279980-49yv65gm 159 26 embryonated embryonate VBN cord-279980-49yv65gm 159 27 chicken chicken NN cord-279980-49yv65gm 159 28 eggs egg NNS cord-279980-49yv65gm 159 29 . . . cord-279980-49yv65gm 160 1 The the DT cord-279980-49yv65gm 160 2 different different JJ cord-279980-49yv65gm 160 3 superscript superscript NN cord-279980-49yv65gm 160 4 letters letter NNS cord-279980-49yv65gm 160 5 in in IN cord-279980-49yv65gm 160 6 the the DT cord-279980-49yv65gm 160 7 same same JJ cord-279980-49yv65gm 160 8 column column NN cord-279980-49yv65gm 160 9 means mean VBZ cord-279980-49yv65gm 160 10 significant significant JJ cord-279980-49yv65gm 160 11 difference difference NN cord-279980-49yv65gm 160 12 ( ( -LRB- cord-279980-49yv65gm 160 13 P p NN cord-279980-49yv65gm 160 14 < < XX cord-279980-49yv65gm 160 15 0.05 0.05 CD cord-279980-49yv65gm 160 16 ) ) -RRB- cord-279980-49yv65gm 160 17 . . . cord-279980-49yv65gm 161 1 the the DT cord-279980-49yv65gm 161 2 virus virus NN cord-279980-49yv65gm 161 3 could could MD cord-279980-49yv65gm 161 4 not not RB cord-279980-49yv65gm 161 5 replicate replicate VB cord-279980-49yv65gm 161 6 in in IN cord-279980-49yv65gm 161 7 the the DT cord-279980-49yv65gm 161 8 house house NN cord-279980-49yv65gm 161 9 flies fly VBZ cord-279980-49yv65gm 161 10 and and CC cord-279980-49yv65gm 161 11 the the DT cord-279980-49yv65gm 161 12 results result NNS cord-279980-49yv65gm 161 13 from from IN cord-279980-49yv65gm 161 14 three three CD cord-279980-49yv65gm 161 15 replicates replicate NNS cord-279980-49yv65gm 161 16 were be VBD cord-279980-49yv65gm 161 17 not not RB cord-279980-49yv65gm 161 18 statistically statistically RB cord-279980-49yv65gm 161 19 significantly significantly RB cord-279980-49yv65gm 161 20 different different JJ cord-279980-49yv65gm 161 21 ( ( -LRB- cord-279980-49yv65gm 161 22 P p NN cord-279980-49yv65gm 161 23 > > XX cord-279980-49yv65gm 161 24 0.05 0.05 CD cord-279980-49yv65gm 161 25 ) ) -RRB- cord-279980-49yv65gm 161 26 . . . cord-279980-49yv65gm 162 1 The the DT cord-279980-49yv65gm 162 2 AI AI NNP cord-279980-49yv65gm 162 3 H5N1 H5N1 NNP cord-279980-49yv65gm 162 4 virus virus NN cord-279980-49yv65gm 162 5 was be VBD cord-279980-49yv65gm 162 6 not not RB cord-279980-49yv65gm 162 7 detected detect VBN cord-279980-49yv65gm 162 8 from from IN cord-279980-49yv65gm 162 9 the the DT cord-279980-49yv65gm 162 10 sham sham NN cord-279980-49yv65gm 162 11 - - HYPH cord-279980-49yv65gm 162 12 inoculated inoculate VBN cord-279980-49yv65gm 162 13 negative negative JJ cord-279980-49yv65gm 162 14 control control NN cord-279980-49yv65gm 162 15 group group NN cord-279980-49yv65gm 162 16 by by IN cord-279980-49yv65gm 162 17 both both DT cord-279980-49yv65gm 162 18 virus virus NN cord-279980-49yv65gm 162 19 titration titration NN cord-279980-49yv65gm 162 20 and and CC cord-279980-49yv65gm 162 21 RRT RRT NNP cord-279980-49yv65gm 162 22 - - HYPH cord-279980-49yv65gm 162 23 PCR PCR NNP cord-279980-49yv65gm 162 24 assay assay NN cord-279980-49yv65gm 162 25 . . . cord-279980-49yv65gm 163 1 The the DT cord-279980-49yv65gm 163 2 highly highly RB cord-279980-49yv65gm 163 3 pathogenic pathogenic JJ cord-279980-49yv65gm 163 4 avian avian JJ cord-279980-49yv65gm 163 5 influenza influenza NN cord-279980-49yv65gm 163 6 virus virus NN cord-279980-49yv65gm 163 7 subtype subtype NN cord-279980-49yv65gm 163 8 H5N1 H5N1 NNP cord-279980-49yv65gm 163 9 causes cause VBZ cord-279980-49yv65gm 163 10 serious serious JJ cord-279980-49yv65gm 163 11 problems problem NNS cord-279980-49yv65gm 163 12 not not RB cord-279980-49yv65gm 163 13 only only RB cord-279980-49yv65gm 163 14 in in IN cord-279980-49yv65gm 163 15 poultry poultry NN cord-279980-49yv65gm 163 16 industries industry NNS cord-279980-49yv65gm 163 17 but but CC cord-279980-49yv65gm 163 18 also also RB cord-279980-49yv65gm 163 19 poses pose VBZ cord-279980-49yv65gm 163 20 a a DT cord-279980-49yv65gm 163 21 threat threat NN cord-279980-49yv65gm 163 22 to to IN cord-279980-49yv65gm 163 23 human human JJ cord-279980-49yv65gm 163 24 health health NN cord-279980-49yv65gm 163 25 . . . cord-279980-49yv65gm 164 1 Generally generally RB cord-279980-49yv65gm 164 2 , , , cord-279980-49yv65gm 164 3 the the DT cord-279980-49yv65gm 164 4 poultry poultry NN cord-279980-49yv65gm 164 5 production production NN cord-279980-49yv65gm 164 6 system system NN cord-279980-49yv65gm 164 7 in in IN cord-279980-49yv65gm 164 8 Thailand Thailand NNP cord-279980-49yv65gm 164 9 can can MD cord-279980-49yv65gm 164 10 be be VB cord-279980-49yv65gm 164 11 divided divide VBN cord-279980-49yv65gm 164 12 into into IN cord-279980-49yv65gm 164 13 four four CD cord-279980-49yv65gm 164 14 sectors sector NNS cord-279980-49yv65gm 164 15 namely namely RB cord-279980-49yv65gm 164 16 , , , cord-279980-49yv65gm 164 17 the the DT cord-279980-49yv65gm 164 18 industrial industrial JJ cord-279980-49yv65gm 164 19 integrated integrate VBN cord-279980-49yv65gm 164 20 system system NN cord-279980-49yv65gm 164 21 , , , cord-279980-49yv65gm 164 22 the the DT cord-279980-49yv65gm 164 23 semi semi JJ cord-279980-49yv65gm 164 24 - - JJ cord-279980-49yv65gm 164 25 vertical vertical JJ cord-279980-49yv65gm 164 26 integrated integrate VBN cord-279980-49yv65gm 164 27 system system NN cord-279980-49yv65gm 164 28 , , , cord-279980-49yv65gm 164 29 extensive extensive JJ cord-279980-49yv65gm 164 30 farming farming NN cord-279980-49yv65gm 164 31 and and CC cord-279980-49yv65gm 164 32 backyard backyard NN cord-279980-49yv65gm 164 33 . . . cord-279980-49yv65gm 165 1 In in IN cord-279980-49yv65gm 165 2 extensive extensive JJ cord-279980-49yv65gm 165 3 farming farming NN cord-279980-49yv65gm 165 4 ( ( -LRB- cord-279980-49yv65gm 165 5 e.g. e.g. RB cord-279980-49yv65gm 165 6 layer layer NN cord-279980-49yv65gm 165 7 hens hen NNS cord-279980-49yv65gm 165 8 , , , cord-279980-49yv65gm 165 9 broilers broiler NNS cord-279980-49yv65gm 165 10 , , , cord-279980-49yv65gm 165 11 ducks duck NNS cord-279980-49yv65gm 165 12 and and CC cord-279980-49yv65gm 165 13 free free RB cord-279980-49yv65gm 165 14 - - HYPH cord-279980-49yv65gm 165 15 grazing graze VBG cord-279980-49yv65gm 165 16 ducks duck NNS cord-279980-49yv65gm 165 17 ) ) -RRB- cord-279980-49yv65gm 165 18 and and CC cord-279980-49yv65gm 165 19 backyards backyard NNS cord-279980-49yv65gm 165 20 ( ( -LRB- cord-279980-49yv65gm 165 21 e.g. e.g. IN cord-279980-49yv65gm 165 22 chickens chicken NNS cord-279980-49yv65gm 165 23 , , , cord-279980-49yv65gm 165 24 ducks duck NNS cord-279980-49yv65gm 165 25 and and CC cord-279980-49yv65gm 165 26 fighting fight VBG cord-279980-49yv65gm 165 27 cocks cock NNS cord-279980-49yv65gm 165 28 ) ) -RRB- cord-279980-49yv65gm 165 29 they -PRON- PRP cord-279980-49yv65gm 165 30 live live VBP cord-279980-49yv65gm 165 31 in in IN cord-279980-49yv65gm 165 32 close close JJ cord-279980-49yv65gm 165 33 proximity proximity NN cord-279980-49yv65gm 165 34 with with IN cord-279980-49yv65gm 165 35 their -PRON- PRP$ cord-279980-49yv65gm 165 36 owners owner NNS cord-279980-49yv65gm 165 37 and and CC cord-279980-49yv65gm 165 38 are be VBP cord-279980-49yv65gm 165 39 freely freely RB cord-279980-49yv65gm 165 40 in in IN cord-279980-49yv65gm 165 41 contact contact NN cord-279980-49yv65gm 165 42 with with IN cord-279980-49yv65gm 165 43 the the DT cord-279980-49yv65gm 165 44 other other JJ cord-279980-49yv65gm 165 45 animals animal NNS cord-279980-49yv65gm 166 1 ( ( -LRB- cord-279980-49yv65gm 166 2 Tiensin Tiensin NNP cord-279980-49yv65gm 166 3 et et NNP cord-279980-49yv65gm 166 4 al al NNP cord-279980-49yv65gm 166 5 . . NNP cord-279980-49yv65gm 166 6 , , , cord-279980-49yv65gm 166 7 2007 2007 CD cord-279980-49yv65gm 166 8 ) ) -RRB- cord-279980-49yv65gm 166 9 . . . cord-279980-49yv65gm 167 1 Then then RB cord-279980-49yv65gm 167 2 , , , cord-279980-49yv65gm 167 3 the the DT cord-279980-49yv65gm 167 4 manure manure NN cord-279980-49yv65gm 167 5 of of IN cord-279980-49yv65gm 167 6 this this DT cord-279980-49yv65gm 167 7 poultry poultry NN cord-279980-49yv65gm 167 8 unit unit NN cord-279980-49yv65gm 167 9 was be VBD cord-279980-49yv65gm 167 10 a a DT cord-279980-49yv65gm 167 11 perfect perfect JJ cord-279980-49yv65gm 167 12 breeding breeding NN cord-279980-49yv65gm 167 13 place place NN cord-279980-49yv65gm 167 14 for for IN cord-279980-49yv65gm 167 15 house house NN cord-279980-49yv65gm 167 16 flies fly NNS cord-279980-49yv65gm 167 17 . . . cord-279980-49yv65gm 168 1 Moreover moreover RB cord-279980-49yv65gm 168 2 , , , cord-279980-49yv65gm 168 3 Thailand Thailand NNP cord-279980-49yv65gm 168 4 's 's POS cord-279980-49yv65gm 168 5 climate climate NN cord-279980-49yv65gm 168 6 is be VBZ cord-279980-49yv65gm 168 7 appropriate appropriate JJ cord-279980-49yv65gm 168 8 for for IN cord-279980-49yv65gm 168 9 the the DT cord-279980-49yv65gm 168 10 development development NN cord-279980-49yv65gm 168 11 of of IN cord-279980-49yv65gm 168 12 house house NN cord-279980-49yv65gm 168 13 flies fly NNS cord-279980-49yv65gm 168 14 . . . cord-279980-49yv65gm 169 1 Previous previous JJ cord-279980-49yv65gm 169 2 studies study NNS cord-279980-49yv65gm 169 3 showed show VBD cord-279980-49yv65gm 169 4 that that IN cord-279980-49yv65gm 169 5 house house NN cord-279980-49yv65gm 169 6 flies fly NNS cord-279980-49yv65gm 169 7 could could MD cord-279980-49yv65gm 169 8 act act VB cord-279980-49yv65gm 169 9 as as IN cord-279980-49yv65gm 169 10 a a DT cord-279980-49yv65gm 169 11 vector vector NN cord-279980-49yv65gm 169 12 for for IN cord-279980-49yv65gm 169 13 transmitting transmit VBG cord-279980-49yv65gm 169 14 disease disease NN cord-279980-49yv65gm 169 15 to to IN cord-279980-49yv65gm 169 16 poultry poultry NN cord-279980-49yv65gm 169 17 farms farm NNS cord-279980-49yv65gm 169 18 and and CC cord-279980-49yv65gm 169 19 flies fly NNS cord-279980-49yv65gm 169 20 readily readily RB cord-279980-49yv65gm 169 21 move move VB cord-279980-49yv65gm 169 22 between between IN cord-279980-49yv65gm 169 23 poultry poultry NN cord-279980-49yv65gm 169 24 buildings building NNS cord-279980-49yv65gm 169 25 ( ( -LRB- cord-279980-49yv65gm 169 26 Lysyk Lysyk NNP cord-279980-49yv65gm 169 27 & & CC cord-279980-49yv65gm 169 28 Axtell Axtell NNP cord-279980-49yv65gm 169 29 , , , cord-279980-49yv65gm 169 30 1986 1986 CD cord-279980-49yv65gm 169 31 ) ) -RRB- cord-279980-49yv65gm 169 32 . . . cord-279980-49yv65gm 170 1 Therefore therefore RB cord-279980-49yv65gm 170 2 , , , cord-279980-49yv65gm 170 3 the the DT cord-279980-49yv65gm 170 4 potential potential NN cord-279980-49yv65gm 170 5 of of IN cord-279980-49yv65gm 170 6 house house NN cord-279980-49yv65gm 170 7 flies fly VBZ cord-279980-49yv65gm 170 8 to to TO cord-279980-49yv65gm 170 9 act act VB cord-279980-49yv65gm 170 10 as as IN cord-279980-49yv65gm 170 11 a a DT cord-279980-49yv65gm 170 12 vector vector NN cord-279980-49yv65gm 170 13 for for IN cord-279980-49yv65gm 170 14 the the DT cord-279980-49yv65gm 170 15 AI AI NNP cord-279980-49yv65gm 170 16 H5N1 H5N1 NNP cord-279980-49yv65gm 170 17 virus virus NN cord-279980-49yv65gm 170 18 was be VBD cord-279980-49yv65gm 170 19 determined determine VBN cord-279980-49yv65gm 170 20 in in IN cord-279980-49yv65gm 170 21 the the DT cord-279980-49yv65gm 170 22 present present JJ cord-279980-49yv65gm 170 23 study study NN cord-279980-49yv65gm 170 24 . . . cord-279980-49yv65gm 171 1 Here here RB cord-279980-49yv65gm 171 2 we -PRON- PRP cord-279980-49yv65gm 171 3 demonstrated demonstrate VBD cord-279980-49yv65gm 171 4 that that IN cord-279980-49yv65gm 171 5 house house NN cord-279980-49yv65gm 171 6 flies fly VBZ cord-279980-49yv65gm 171 7 that that WDT cord-279980-49yv65gm 171 8 consumed consume VBD cord-279980-49yv65gm 171 9 food food NN cord-279980-49yv65gm 171 10 contaminated contaminate VBN cord-279980-49yv65gm 171 11 with with IN cord-279980-49yv65gm 171 12 AI AI NNP cord-279980-49yv65gm 171 13 H5N1 H5N1 NNP cord-279980-49yv65gm 171 14 could could MD cord-279980-49yv65gm 171 15 carry carry VB cord-279980-49yv65gm 171 16 the the DT cord-279980-49yv65gm 171 17 virus virus NN cord-279980-49yv65gm 171 18 within within IN cord-279980-49yv65gm 171 19 their -PRON- PRP$ cord-279980-49yv65gm 171 20 bodies body NNS cord-279980-49yv65gm 171 21 for for IN cord-279980-49yv65gm 171 22 a a DT cord-279980-49yv65gm 171 23 long long JJ cord-279980-49yv65gm 171 24 period period NN cord-279980-49yv65gm 171 25 of of IN cord-279980-49yv65gm 171 26 time time NN cord-279980-49yv65gm 171 27 at at RB cord-279980-49yv65gm 171 28 least least JJS cord-279980-49yv65gm 171 29 72 72 CD cord-279980-49yv65gm 171 30 h h NN cord-279980-49yv65gm 171 31 post post NN cord-279980-49yv65gm 171 32 - - NN cord-279980-49yv65gm 171 33 exposure exposure NN cord-279980-49yv65gm 171 34 . . . cord-279980-49yv65gm 172 1 The the DT cord-279980-49yv65gm 172 2 virus virus NN cord-279980-49yv65gm 172 3 was be VBD cord-279980-49yv65gm 172 4 detected detect VBN cord-279980-49yv65gm 172 5 both both CC cord-279980-49yv65gm 172 6 in in IN cord-279980-49yv65gm 172 7 the the DT cord-279980-49yv65gm 172 8 homogenates homogenate NNS cord-279980-49yv65gm 172 9 of of IN cord-279980-49yv65gm 172 10 whole whole JJ cord-279980-49yv65gm 172 11 flies fly NNS cord-279980-49yv65gm 172 12 and and CC cord-279980-49yv65gm 172 13 the the DT cord-279980-49yv65gm 172 14 external external JJ cord-279980-49yv65gm 172 15 surfaces surface NNS cord-279980-49yv65gm 172 16 of of IN cord-279980-49yv65gm 172 17 flies fly NNS cord-279980-49yv65gm 172 18 at at IN cord-279980-49yv65gm 172 19 high high JJ cord-279980-49yv65gm 172 20 levels level NNS cord-279980-49yv65gm 172 21 . . . cord-279980-49yv65gm 173 1 Moreover moreover RB cord-279980-49yv65gm 173 2 , , , cord-279980-49yv65gm 173 3 virus virus NN cord-279980-49yv65gm 173 4 titres titre NNS cord-279980-49yv65gm 173 5 of of IN cord-279980-49yv65gm 173 6 a a DT cord-279980-49yv65gm 173 7 whole whole JJ cord-279980-49yv65gm 173 8 fly fly NN cord-279980-49yv65gm 173 9 homogenate homogenate NNP cord-279980-49yv65gm 173 10 compared compare VBN cord-279980-49yv65gm 173 11 with with IN cord-279980-49yv65gm 173 12 that that DT cord-279980-49yv65gm 173 13 of of IN cord-279980-49yv65gm 173 14 washing wash VBG cord-279980-49yv65gm 173 15 fluid fluid NN cord-279980-49yv65gm 173 16 revealed reveal VBD cord-279980-49yv65gm 173 17 that that IN cord-279980-49yv65gm 173 18 the the DT cord-279980-49yv65gm 173 19 viruses virus NNS cord-279980-49yv65gm 173 20 could could MD cord-279980-49yv65gm 173 21 be be VB cord-279980-49yv65gm 173 22 detected detect VBN cord-279980-49yv65gm 173 23 in in IN cord-279980-49yv65gm 173 24 homogenates homogenate NNS cord-279980-49yv65gm 173 25 of of IN cord-279980-49yv65gm 173 26 whole whole JJ cord-279980-49yv65gm 173 27 flies fly NNS cord-279980-49yv65gm 173 28 for for IN cord-279980-49yv65gm 173 29 up up IN cord-279980-49yv65gm 173 30 to to TO cord-279980-49yv65gm 173 31 96 96 CD cord-279980-49yv65gm 173 32 h h NN cord-279980-49yv65gm 173 33 post post NN cord-279980-49yv65gm 173 34 - - NN cord-279980-49yv65gm 173 35 exposure exposure NN cord-279980-49yv65gm 173 36 , , , cord-279980-49yv65gm 173 37 whereas whereas IN cord-279980-49yv65gm 173 38 these these DT cord-279980-49yv65gm 173 39 viruses virus NNS cord-279980-49yv65gm 173 40 could could MD cord-279980-49yv65gm 173 41 be be VB cord-279980-49yv65gm 173 42 detected detect VBN cord-279980-49yv65gm 173 43 in in IN cord-279980-49yv65gm 173 44 external external JJ cord-279980-49yv65gm 173 45 surfaces surface NNS cord-279980-49yv65gm 173 46 of of IN cord-279980-49yv65gm 173 47 house house NN cord-279980-49yv65gm 173 48 flies fly VBZ cord-279980-49yv65gm 173 49 for for IN cord-279980-49yv65gm 173 50 only only RB cord-279980-49yv65gm 173 51 up up IN cord-279980-49yv65gm 173 52 to to TO cord-279980-49yv65gm 173 53 24 24 CD cord-279980-49yv65gm 173 54 h h NN cord-279980-49yv65gm 173 55 postexposure postexposure NN cord-279980-49yv65gm 173 56 ( ( -LRB- cord-279980-49yv65gm 173 57 Table table NN cord-279980-49yv65gm 173 58 1 1 CD cord-279980-49yv65gm 173 59 ) ) -RRB- cord-279980-49yv65gm 173 60 . . . cord-279980-49yv65gm 174 1 The the DT cord-279980-49yv65gm 174 2 capacity capacity NN cord-279980-49yv65gm 174 3 of of IN cord-279980-49yv65gm 174 4 a a DT cord-279980-49yv65gm 174 5 house house NN cord-279980-49yv65gm 174 6 fly fly NN cord-279980-49yv65gm 174 7 to to TO cord-279980-49yv65gm 174 8 carry carry VB cord-279980-49yv65gm 174 9 the the DT cord-279980-49yv65gm 174 10 AI AI NNP cord-279980-49yv65gm 174 11 H5N1 H5N1 NNP cord-279980-49yv65gm 174 12 virus virus NN cord-279980-49yv65gm 174 13 via via IN cord-279980-49yv65gm 174 14 whole whole JJ cord-279980-49yv65gm 174 15 fly fly NN cord-279980-49yv65gm 174 16 homogenate homogenate NNP cord-279980-49yv65gm 174 17 was be VBD cord-279980-49yv65gm 174 18 significantly significantly RB cord-279980-49yv65gm 174 19 higher high JJR cord-279980-49yv65gm 174 20 than than IN cord-279980-49yv65gm 174 21 that that DT cord-279980-49yv65gm 174 22 of of IN cord-279980-49yv65gm 174 23 the the DT cord-279980-49yv65gm 174 24 external external JJ cord-279980-49yv65gm 174 25 surface surface NN cord-279980-49yv65gm 174 26 ( ( -LRB- cord-279980-49yv65gm 174 27 P p NN cord-279980-49yv65gm 174 28 < < XX cord-279980-49yv65gm 174 29 0.05 0.05 CD cord-279980-49yv65gm 174 30 ) ) -RRB- cord-279980-49yv65gm 174 31 . . . cord-279980-49yv65gm 175 1 Our -PRON- PRP$ cord-279980-49yv65gm 175 2 finding finding NN cord-279980-49yv65gm 175 3 is be VBZ cord-279980-49yv65gm 175 4 consistent consistent JJ cord-279980-49yv65gm 175 5 with with IN cord-279980-49yv65gm 175 6 Otake Otake NNP cord-279980-49yv65gm 175 7 et et NNP cord-279980-49yv65gm 175 8 al al NNP cord-279980-49yv65gm 175 9 . . . cord-279980-49yv65gm 176 1 ( ( -LRB- cord-279980-49yv65gm 176 2 2003 2003 CD cord-279980-49yv65gm 176 3 ) ) -RRB- cord-279980-49yv65gm 176 4 that that WDT cord-279980-49yv65gm 176 5 found find VBD cord-279980-49yv65gm 176 6 viable viable JJ cord-279980-49yv65gm 176 7 porcine porcine JJ cord-279980-49yv65gm 176 8 reproductive reproductive JJ cord-279980-49yv65gm 176 9 and and CC cord-279980-49yv65gm 176 10 respiratory respiratory JJ cord-279980-49yv65gm 176 11 syndrome syndrome NN cord-279980-49yv65gm 176 12 virus virus NN cord-279980-49yv65gm 176 13 ( ( -LRB- cord-279980-49yv65gm 176 14 PRRSV PRRSV NNP cord-279980-49yv65gm 176 15 ) ) -RRB- cord-279980-49yv65gm 176 16 in in IN cord-279980-49yv65gm 176 17 the the DT cord-279980-49yv65gm 176 18 internal internal JJ cord-279980-49yv65gm 176 19 organs organ NNS cord-279980-49yv65gm 176 20 of of IN cord-279980-49yv65gm 176 21 house house NN cord-279980-49yv65gm 176 22 flies fly VBZ cord-279980-49yv65gm 176 23 higher high JJR cord-279980-49yv65gm 176 24 than than IN cord-279980-49yv65gm 176 25 the the DT cord-279980-49yv65gm 176 26 external external JJ cord-279980-49yv65gm 176 27 surface surface NN cord-279980-49yv65gm 176 28 . . . cord-279980-49yv65gm 177 1 A a DT cord-279980-49yv65gm 177 2 separate separate JJ cord-279980-49yv65gm 177 3 study study NN cord-279980-49yv65gm 177 4 detected detect VBD cord-279980-49yv65gm 177 5 higher high JJR cord-279980-49yv65gm 177 6 levels level NNS cord-279980-49yv65gm 177 7 of of IN cord-279980-49yv65gm 177 8 Exotic exotic JJ cord-279980-49yv65gm 177 9 Newcastle Newcastle NNP cord-279980-49yv65gm 177 10 disease disease NN cord-279980-49yv65gm 177 11 virus virus NN cord-279980-49yv65gm 177 12 ( ( -LRB- cord-279980-49yv65gm 177 13 ENDV ENDV NNP cord-279980-49yv65gm 177 14 ) ) -RRB- cord-279980-49yv65gm 177 15 from from IN cord-279980-49yv65gm 177 16 the the DT cord-279980-49yv65gm 177 17 whole whole JJ cord-279980-49yv65gm 177 18 house house NN cord-279980-49yv65gm 177 19 fly fly VB cord-279980-49yv65gm 177 20 homogenate homogenate NNP cord-279980-49yv65gm 177 21 than than IN cord-279980-49yv65gm 177 22 the the DT cord-279980-49yv65gm 177 23 level level NN cord-279980-49yv65gm 177 24 of of IN cord-279980-49yv65gm 177 25 virus virus NN cord-279980-49yv65gm 177 26 from from IN cord-279980-49yv65gm 177 27 the the DT cord-279980-49yv65gm 177 28 body body NN cord-279980-49yv65gm 177 29 surface surface NN cord-279980-49yv65gm 177 30 ( ( -LRB- cord-279980-49yv65gm 177 31 Chakrabarti Chakrabarti NNP cord-279980-49yv65gm 177 32 et et NNP cord-279980-49yv65gm 177 33 al al NNP cord-279980-49yv65gm 177 34 . . NNP cord-279980-49yv65gm 177 35 , , , cord-279980-49yv65gm 177 36 2008 2008 CD cord-279980-49yv65gm 177 37 ) ) -RRB- cord-279980-49yv65gm 177 38 . . . cord-279980-49yv65gm 178 1 In in IN cord-279980-49yv65gm 178 2 general general JJ cord-279980-49yv65gm 178 3 , , , cord-279980-49yv65gm 178 4 the the DT cord-279980-49yv65gm 178 5 avian avian JJ cord-279980-49yv65gm 178 6 influenza influenza NN cord-279980-49yv65gm 178 7 virus virus NN cord-279980-49yv65gm 178 8 can can MD cord-279980-49yv65gm 178 9 survive survive VB cord-279980-49yv65gm 178 10 in in IN cord-279980-49yv65gm 178 11 an an DT cord-279980-49yv65gm 178 12 environment environment NN cord-279980-49yv65gm 178 13 for for IN cord-279980-49yv65gm 178 14 a a DT cord-279980-49yv65gm 178 15 period period NN cord-279980-49yv65gm 178 16 of of IN cord-279980-49yv65gm 178 17 time time NN cord-279980-49yv65gm 178 18 ( ( -LRB- cord-279980-49yv65gm 178 19 Swayne Swayne NNP cord-279980-49yv65gm 178 20 & & CC cord-279980-49yv65gm 178 21 Halvorson Halvorson NNP cord-279980-49yv65gm 178 22 , , , cord-279980-49yv65gm 178 23 2003 2003 CD cord-279980-49yv65gm 178 24 ) ) -RRB- cord-279980-49yv65gm 178 25 . . . cord-279980-49yv65gm 179 1 Optimum optimum JJ cord-279980-49yv65gm 179 2 humidity humidity NN cord-279980-49yv65gm 179 3 , , , cord-279980-49yv65gm 179 4 temperature temperature NN cord-279980-49yv65gm 179 5 and and CC cord-279980-49yv65gm 179 6 organic organic JJ cord-279980-49yv65gm 179 7 materials material NNS cord-279980-49yv65gm 179 8 including include VBG cord-279980-49yv65gm 179 9 nasal nasal NN cord-279980-49yv65gm 179 10 discharge discharge NN cord-279980-49yv65gm 179 11 and and CC cord-279980-49yv65gm 179 12 faeces faece NNS cord-279980-49yv65gm 179 13 can can MD cord-279980-49yv65gm 179 14 prolong prolong VB cord-279980-49yv65gm 179 15 the the DT cord-279980-49yv65gm 179 16 survival survival NN cord-279980-49yv65gm 179 17 time time NN cord-279980-49yv65gm 179 18 of of IN cord-279980-49yv65gm 179 19 many many JJ cord-279980-49yv65gm 179 20 viruses virus NNS cord-279980-49yv65gm 179 21 ( ( -LRB- cord-279980-49yv65gm 179 22 Pantin Pantin NNP cord-279980-49yv65gm 179 23 - - HYPH cord-279980-49yv65gm 179 24 Jackwood Jackwood NNP cord-279980-49yv65gm 179 25 & & CC cord-279980-49yv65gm 179 26 Swayne Swayne NNP cord-279980-49yv65gm 179 27 , , , cord-279980-49yv65gm 179 28 2009 2009 CD cord-279980-49yv65gm 179 29 ) ) -RRB- cord-279980-49yv65gm 179 30 . . . cord-279980-49yv65gm 180 1 The the DT cord-279980-49yv65gm 180 2 AI AI NNP cord-279980-49yv65gm 180 3 H5N2 H5N2 NNP cord-279980-49yv65gm 180 4 virus virus NN cord-279980-49yv65gm 180 5 can can MD cord-279980-49yv65gm 180 6 survive survive VB cord-279980-49yv65gm 180 7 in in IN cord-279980-49yv65gm 180 8 faeces faece NNS cord-279980-49yv65gm 180 9 at at IN cord-279980-49yv65gm 180 10 4 4 CD cord-279980-49yv65gm 180 11 • • NN cord-279980-49yv65gm 180 12 C c NN cord-279980-49yv65gm 180 13 for for IN cord-279980-49yv65gm 180 14 30 30 CD cord-279980-49yv65gm 180 15 - - SYM cord-279980-49yv65gm 180 16 35 35 CD cord-279980-49yv65gm 180 17 days day NNS cord-279980-49yv65gm 180 18 and and CC cord-279980-49yv65gm 180 19 at at IN cord-279980-49yv65gm 180 20 20 20 CD cord-279980-49yv65gm 181 1 • • NNP cord-279980-49yv65gm 181 2 C C NNP cord-279980-49yv65gm 181 3 for for IN cord-279980-49yv65gm 181 4 7 7 CD cord-279980-49yv65gm 181 5 days day NNS cord-279980-49yv65gm 181 6 ( ( -LRB- cord-279980-49yv65gm 181 7 Swayne Swayne NNP cord-279980-49yv65gm 181 8 & & CC cord-279980-49yv65gm 181 9 Halvorson Halvorson NNP cord-279980-49yv65gm 181 10 , , , cord-279980-49yv65gm 181 11 2003 2003 CD cord-279980-49yv65gm 181 12 ) ) -RRB- cord-279980-49yv65gm 181 13 . . . cord-279980-49yv65gm 182 1 In in IN cord-279980-49yv65gm 182 2 contrast contrast NN cord-279980-49yv65gm 182 3 , , , cord-279980-49yv65gm 182 4 the the DT cord-279980-49yv65gm 182 5 AI AI NNP cord-279980-49yv65gm 182 6 H5N1 H5N1 NNP cord-279980-49yv65gm 182 7 virus virus NN cord-279980-49yv65gm 182 8 can can MD cord-279980-49yv65gm 182 9 survive survive VB cord-279980-49yv65gm 182 10 at at IN cord-279980-49yv65gm 182 11 25 25 CD cord-279980-49yv65gm 182 12 • • NNS cord-279980-49yv65gm 182 13 C C NNP cord-279980-49yv65gm 182 14 for for IN cord-279980-49yv65gm 182 15 24 24 CD cord-279980-49yv65gm 182 16 h h NN cord-279980-49yv65gm 182 17 , , , cord-279980-49yv65gm 182 18 and and CC cord-279980-49yv65gm 182 19 at at IN cord-279980-49yv65gm 182 20 40 40 CD cord-279980-49yv65gm 182 21 • • NN cord-279980-49yv65gm 182 22 C C NNP cord-279980-49yv65gm 182 23 for for IN cord-279980-49yv65gm 182 24 15 15 CD cord-279980-49yv65gm 182 25 min min NN cord-279980-49yv65gm 183 1 ( ( -LRB- cord-279980-49yv65gm 183 2 Chumpolbanchorn Chumpolbanchorn NNP cord-279980-49yv65gm 183 3 et et NNP cord-279980-49yv65gm 183 4 al al NNP cord-279980-49yv65gm 183 5 . . NNP cord-279980-49yv65gm 183 6 , , , cord-279980-49yv65gm 183 7 2006 2006 CD cord-279980-49yv65gm 183 8 ) ) -RRB- cord-279980-49yv65gm 183 9 . . . cord-279980-49yv65gm 184 1 Jeong Jeong NNP cord-279980-49yv65gm 184 2 et et NNP cord-279980-49yv65gm 184 3 al al NNP cord-279980-49yv65gm 184 4 . . . cord-279980-49yv65gm 185 1 ( ( -LRB- cord-279980-49yv65gm 185 2 2009 2009 CD cord-279980-49yv65gm 185 3 ) ) -RRB- cord-279980-49yv65gm 185 4 found find VBD cord-279980-49yv65gm 185 5 that that IN cord-279980-49yv65gm 185 6 experimental experimental JJ cord-279980-49yv65gm 185 7 chickens chicken NNS cord-279980-49yv65gm 185 8 inoculated inoculate VBN cord-279980-49yv65gm 185 9 with with IN cord-279980-49yv65gm 185 10 10 10 CD cord-279980-49yv65gm 185 11 6.5 6.5 CD cord-279980-49yv65gm 185 12 EID EID NNP cord-279980-49yv65gm 185 13 50 50 CD cord-279980-49yv65gm 185 14 of of IN cord-279980-49yv65gm 185 15 A a NN cord-279980-49yv65gm 186 1 /Chicken /Chicken NNP cord-279980-49yv65gm 186 2 /Korea /Korea . cord-279980-49yv65gm 187 1 / / NFP cord-279980-49yv65gm 188 1 IS/06 IS/06 NNP cord-279980-49yv65gm 188 2 ( ( -LRB- cord-279980-49yv65gm 188 3 H5N1 H5N1 NNP cord-279980-49yv65gm 188 4 ) ) -RRB- cord-279980-49yv65gm 188 5 can can MD cord-279980-49yv65gm 188 6 shed shed VB cord-279980-49yv65gm 188 7 a a DT cord-279980-49yv65gm 188 8 virus virus NN cord-279980-49yv65gm 188 9 from from IN cord-279980-49yv65gm 188 10 oropharyngeal oropharyngeal NN cord-279980-49yv65gm 188 11 and and CC cord-279980-49yv65gm 188 12 cloaca cloaca NN cord-279980-49yv65gm 188 13 for for IN cord-279980-49yv65gm 188 14 3 3 CD cord-279980-49yv65gm 188 15 days day NNS cord-279980-49yv65gm 188 16 at at IN cord-279980-49yv65gm 188 17 virus virus NN cord-279980-49yv65gm 188 18 titres titre NNS cord-279980-49yv65gm 188 19 of of IN cord-279980-49yv65gm 188 20 10 10 CD cord-279980-49yv65gm 188 21 4.6 4.6 CD cord-279980-49yv65gm 188 22 and and CC cord-279980-49yv65gm 188 23 10 10 CD cord-279980-49yv65gm 188 24 2.4 2.4 CD cord-279980-49yv65gm 188 25 TCID TCID NNP cord-279980-49yv65gm 188 26 50 50 CD cord-279980-49yv65gm 188 27 /mL /mL NFP cord-279980-49yv65gm 188 28 , , , cord-279980-49yv65gm 188 29 respectively respectively RB cord-279980-49yv65gm 188 30 . . . cord-279980-49yv65gm 189 1 Other other JJ cord-279980-49yv65gm 189 2 poultry poultry NN cord-279980-49yv65gm 189 3 species specie NNS cord-279980-49yv65gm 189 4 infected infect VBN cord-279980-49yv65gm 189 5 with with IN cord-279980-49yv65gm 189 6 H5N1 H5N1 NNP cord-279980-49yv65gm 189 7 including include VBG cord-279980-49yv65gm 189 8 ducks duck NNS cord-279980-49yv65gm 189 9 and and CC cord-279980-49yv65gm 189 10 quails quail NNS cord-279980-49yv65gm 189 11 can can MD cord-279980-49yv65gm 189 12 shed shed VB cord-279980-49yv65gm 189 13 virus virus NN cord-279980-49yv65gm 189 14 up up IN cord-279980-49yv65gm 189 15 to to IN cord-279980-49yv65gm 189 16 4 4 CD cord-279980-49yv65gm 189 17 and and CC cord-279980-49yv65gm 189 18 6 6 CD cord-279980-49yv65gm 189 19 days day NNS cord-279980-49yv65gm 189 20 , , , cord-279980-49yv65gm 189 21 respectively respectively RB cord-279980-49yv65gm 189 22 at at IN cord-279980-49yv65gm 189 23 titres titre NNS cord-279980-49yv65gm 189 24 of of IN cord-279980-49yv65gm 189 25 oropharyngeal oropharyngeal JJ cord-279980-49yv65gm 189 26 swabs swab NNS cord-279980-49yv65gm 189 27 is be VBZ cord-279980-49yv65gm 189 28 10 10 CD cord-279980-49yv65gm 189 29 3.2 3.2 CD cord-279980-49yv65gm 189 30 and and CC cord-279980-49yv65gm 189 31 10 10 CD cord-279980-49yv65gm 189 32 6.0 6.0 CD cord-279980-49yv65gm 189 33 TCID TCID NNP cord-279980-49yv65gm 189 34 50 50 CD cord-279980-49yv65gm 189 35 /mL /mL NFP cord-279980-49yv65gm 189 36 , , , cord-279980-49yv65gm 189 37 respectively respectively RB cord-279980-49yv65gm 189 38 . . . cord-279980-49yv65gm 190 1 Pantin Pantin NNP cord-279980-49yv65gm 190 2 - - HYPH cord-279980-49yv65gm 190 3 Jackwood Jackwood NNP cord-279980-49yv65gm 190 4 & & CC cord-279980-49yv65gm 190 5 Swayne Swayne NNP cord-279980-49yv65gm 190 6 ( ( -LRB- cord-279980-49yv65gm 190 7 2009 2009 CD cord-279980-49yv65gm 190 8 ) ) -RRB- cord-279980-49yv65gm 190 9 demonstrated demonstrate VBD cord-279980-49yv65gm 190 10 that that IN cord-279980-49yv65gm 190 11 Pekin Pekin NNP cord-279980-49yv65gm 190 12 ducks duck NNS cord-279980-49yv65gm 190 13 intranasally intranasally RB cord-279980-49yv65gm 190 14 inoculated inoculate VBN cord-279980-49yv65gm 190 15 with with IN cord-279980-49yv65gm 190 16 the the DT cord-279980-49yv65gm 190 17 AI AI NNP cord-279980-49yv65gm 190 18 H5N1 H5N1 NNP cord-279980-49yv65gm 190 19 virus virus NN cord-279980-49yv65gm 190 20 could could MD cord-279980-49yv65gm 190 21 shed shed VB cord-279980-49yv65gm 190 22 virus virus NN cord-279980-49yv65gm 190 23 from from IN cord-279980-49yv65gm 190 24 oral oral JJ cord-279980-49yv65gm 190 25 and and CC cord-279980-49yv65gm 190 26 cloaca cloaca NN cord-279980-49yv65gm 190 27 at at IN cord-279980-49yv65gm 190 28 titres titre NNS cord-279980-49yv65gm 190 29 of of IN cord-279980-49yv65gm 190 30 10 10 CD cord-279980-49yv65gm 190 31 6.5 6.5 CD cord-279980-49yv65gm 190 32 and and CC cord-279980-49yv65gm 190 33 10 10 CD cord-279980-49yv65gm 190 34 3.3 3.3 CD cord-279980-49yv65gm 190 35 EID EID NNP cord-279980-49yv65gm 190 36 50 50 CD cord-279980-49yv65gm 190 37 , , , cord-279980-49yv65gm 190 38 respectively respectively RB cord-279980-49yv65gm 190 39 . . . cord-279980-49yv65gm 191 1 Interestingly interestingly RB cord-279980-49yv65gm 191 2 , , , cord-279980-49yv65gm 191 3 the the DT cord-279980-49yv65gm 191 4 results result NNS cord-279980-49yv65gm 191 5 of of IN cord-279980-49yv65gm 191 6 the the DT cord-279980-49yv65gm 191 7 present present JJ cord-279980-49yv65gm 191 8 study study NN cord-279980-49yv65gm 191 9 showed show VBD cord-279980-49yv65gm 191 10 that that IN cord-279980-49yv65gm 191 11 an an DT cord-279980-49yv65gm 191 12 individual individual JJ cord-279980-49yv65gm 191 13 house house NN cord-279980-49yv65gm 191 14 fly fly VB cord-279980-49yv65gm 191 15 can can MD cord-279980-49yv65gm 191 16 uptake uptake VB cord-279980-49yv65gm 191 17 the the DT cord-279980-49yv65gm 191 18 virus virus NN cord-279980-49yv65gm 191 19 in in IN cord-279980-49yv65gm 191 20 approximately approximately RB cord-279980-49yv65gm 191 21 10 10 CD cord-279980-49yv65gm 191 22 5.43 5.43 CD cord-279980-49yv65gm 191 23 ELD ELD NNP cord-279980-49yv65gm 191 24 50 50 CD cord-279980-49yv65gm 191 25 /mL /mL NNS cord-279980-49yv65gm 191 26 out out IN cord-279980-49yv65gm 191 27 of of IN cord-279980-49yv65gm 191 28 1 1 CD cord-279980-49yv65gm 191 29 × × CD cord-279980-49yv65gm 191 30 10 10 CD cord-279980-49yv65gm 191 31 9.2 9.2 CD cord-279980-49yv65gm 191 32 ELD ELD NNP cord-279980-49yv65gm 191 33 50 50 CD cord-279980-49yv65gm 191 34 /mL /mL : cord-279980-49yv65gm 191 35 of of IN cord-279980-49yv65gm 191 36 infectious infectious JJ cord-279980-49yv65gm 191 37 mixture mixture NN cord-279980-49yv65gm 191 38 or or CC cord-279980-49yv65gm 191 39 approximately approximately RB cord-279980-49yv65gm 191 40 0.02 0.02 CD cord-279980-49yv65gm 191 41 % % NN cord-279980-49yv65gm 191 42 of of IN cord-279980-49yv65gm 191 43 the the DT cord-279980-49yv65gm 191 44 total total JJ cord-279980-49yv65gm 191 45 virus virus NN cord-279980-49yv65gm 191 46 when when WRB cord-279980-49yv65gm 191 47 it -PRON- PRP cord-279980-49yv65gm 191 48 was be VBD cord-279980-49yv65gm 191 49 kept keep VBN cord-279980-49yv65gm 191 50 at at IN cord-279980-49yv65gm 191 51 room room NN cord-279980-49yv65gm 191 52 temperature temperature NN cord-279980-49yv65gm 191 53 . . . cord-279980-49yv65gm 192 1 Thus thus RB cord-279980-49yv65gm 192 2 , , , cord-279980-49yv65gm 192 3 our -PRON- PRP$ cord-279980-49yv65gm 192 4 data datum NNS cord-279980-49yv65gm 192 5 suggest suggest VBP cord-279980-49yv65gm 192 6 that that IN cord-279980-49yv65gm 192 7 house house NN cord-279980-49yv65gm 192 8 flies fly NNS cord-279980-49yv65gm 192 9 can can MD cord-279980-49yv65gm 192 10 carry carry VB cord-279980-49yv65gm 192 11 a a DT cord-279980-49yv65gm 192 12 similar similar JJ cord-279980-49yv65gm 192 13 amount amount NN cord-279980-49yv65gm 192 14 of of IN cord-279980-49yv65gm 192 15 the the DT cord-279980-49yv65gm 192 16 AI AI NNP cord-279980-49yv65gm 192 17 H5N1 H5N1 NNP cord-279980-49yv65gm 192 18 virus virus NN cord-279980-49yv65gm 192 19 to to IN cord-279980-49yv65gm 192 20 the the DT cord-279980-49yv65gm 192 21 amount amount NN cord-279980-49yv65gm 192 22 of of IN cord-279980-49yv65gm 192 23 virus virus NN cord-279980-49yv65gm 192 24 shed shed VBN cord-279980-49yv65gm 192 25 from from IN cord-279980-49yv65gm 192 26 H5N1 H5N1 NNP cord-279980-49yv65gm 192 27 experimentally experimentally RB cord-279980-49yv65gm 192 28 infected infect VBD cord-279980-49yv65gm 192 29 avian avian JJ cord-279980-49yv65gm 192 30 species specie NNS cord-279980-49yv65gm 192 31 such such JJ cord-279980-49yv65gm 192 32 as as IN cord-279980-49yv65gm 192 33 chickens chicken NNS cord-279980-49yv65gm 192 34 , , , cord-279980-49yv65gm 192 35 quails quail NNS cord-279980-49yv65gm 192 36 and and CC cord-279980-49yv65gm 192 37 ducks duck NNS cord-279980-49yv65gm 192 38 . . . cord-279980-49yv65gm 193 1 However however RB cord-279980-49yv65gm 193 2 , , , cord-279980-49yv65gm 193 3 transmission transmission NN cord-279980-49yv65gm 193 4 studies study NNS cord-279980-49yv65gm 193 5 should should MD cord-279980-49yv65gm 193 6 be be VB cord-279980-49yv65gm 193 7 performed perform VBN cord-279980-49yv65gm 193 8 . . . cord-279980-49yv65gm 194 1 In in IN cord-279980-49yv65gm 194 2 addition addition NN cord-279980-49yv65gm 194 3 , , , cord-279980-49yv65gm 194 4 an an DT cord-279980-49yv65gm 194 5 individual individual JJ cord-279980-49yv65gm 194 6 house house NN cord-279980-49yv65gm 194 7 fly fly VB cord-279980-49yv65gm 194 8 can can MD cord-279980-49yv65gm 194 9 fly fly VB cord-279980-49yv65gm 194 10 up up IN cord-279980-49yv65gm 194 11 to to TO cord-279980-49yv65gm 194 12 11.8 11.8 CD cord-279980-49yv65gm 194 13 km km NNS cord-279980-49yv65gm 194 14 within within IN cord-279980-49yv65gm 194 15 24 24 CD cord-279980-49yv65gm 194 16 h h NN cord-279980-49yv65gm 194 17 ( ( -LRB- cord-279980-49yv65gm 194 18 Greenberg Greenberg NNP cord-279980-49yv65gm 194 19 , , , cord-279980-49yv65gm 194 20 1973 1973 CD cord-279980-49yv65gm 194 21 ) ) -RRB- cord-279980-49yv65gm 194 22 . . . cord-279980-49yv65gm 195 1 Therefore therefore RB cord-279980-49yv65gm 195 2 , , , cord-279980-49yv65gm 195 3 house house NN cord-279980-49yv65gm 195 4 flies fly NNS cord-279980-49yv65gm 195 5 may may MD cord-279980-49yv65gm 195 6 serve serve VB cord-279980-49yv65gm 195 7 as as IN cord-279980-49yv65gm 195 8 potential potential JJ cord-279980-49yv65gm 195 9 mechanical mechanical JJ cord-279980-49yv65gm 195 10 vectors vector NNS cord-279980-49yv65gm 195 11 which which WDT cord-279980-49yv65gm 195 12 spread spread VBD cord-279980-49yv65gm 195 13 the the DT cord-279980-49yv65gm 195 14 AI AI NNP cord-279980-49yv65gm 195 15 H5N1 H5N1 NNP cord-279980-49yv65gm 195 16 virus virus NN cord-279980-49yv65gm 195 17 to to IN cord-279980-49yv65gm 195 18 poultry poultry NN cord-279980-49yv65gm 195 19 farms farm NNS cord-279980-49yv65gm 195 20 in in IN cord-279980-49yv65gm 195 21 the the DT cord-279980-49yv65gm 195 22 nearby nearby JJ cord-279980-49yv65gm 195 23 area area NN cord-279980-49yv65gm 195 24 similar similar JJ cord-279980-49yv65gm 195 25 to to TO cord-279980-49yv65gm 195 26 blow blow VB cord-279980-49yv65gm 195 27 flies fly NNS cord-279980-49yv65gm 195 28 ( ( -LRB- cord-279980-49yv65gm 195 29 Calliphora Calliphora NNP cord-279980-49yv65gm 195 30 nigribarbis nigribarbis NNP cord-279980-49yv65gm 195 31 ) ) -RRB- cord-279980-49yv65gm 195 32 that that WDT cord-279980-49yv65gm 195 33 was be VBD cord-279980-49yv65gm 195 34 shown show VBN cord-279980-49yv65gm 195 35 to to TO cord-279980-49yv65gm 195 36 be be VB cord-279980-49yv65gm 195 37 mechanical mechanical JJ cord-279980-49yv65gm 195 38 vectors vector NNS cord-279980-49yv65gm 195 39 for for IN cord-279980-49yv65gm 195 40 AIV AIV NNP cord-279980-49yv65gm 195 41 in in IN cord-279980-49yv65gm 195 42 Japan Japan NNP cord-279980-49yv65gm 196 1 ( ( -LRB- cord-279980-49yv65gm 196 2 Sawabe Sawabe NNP cord-279980-49yv65gm 196 3 et et NNP cord-279980-49yv65gm 196 4 al al NNP cord-279980-49yv65gm 196 5 . . NNP cord-279980-49yv65gm 196 6 , , , cord-279980-49yv65gm 196 7 2009 2009 CD cord-279980-49yv65gm 196 8 ) ) -RRB- cord-279980-49yv65gm 196 9 . . . cord-279980-49yv65gm 197 1 The the DT cord-279980-49yv65gm 197 2 results result NNS cord-279980-49yv65gm 197 3 of of IN cord-279980-49yv65gm 197 4 experiment experiment NN cord-279980-49yv65gm 197 5 3 3 CD cord-279980-49yv65gm 197 6 revealed reveal VBD cord-279980-49yv65gm 197 7 that that IN cord-279980-49yv65gm 197 8 the the DT cord-279980-49yv65gm 197 9 AI AI NNP cord-279980-49yv65gm 197 10 H5N1 H5N1 NNP cord-279980-49yv65gm 197 11 virus virus NN cord-279980-49yv65gm 197 12 could could MD cord-279980-49yv65gm 197 13 be be VB cord-279980-49yv65gm 197 14 detected detect VBN cord-279980-49yv65gm 197 15 within within IN cord-279980-49yv65gm 197 16 the the DT cord-279980-49yv65gm 197 17 fed fed NNP cord-279980-49yv65gm 197 18 house house NN cord-279980-49yv65gm 197 19 flies fly VBZ cord-279980-49yv65gm 197 20 for for IN cord-279980-49yv65gm 197 21 up up IN cord-279980-49yv65gm 197 22 to to TO cord-279980-49yv65gm 197 23 72 72 CD cord-279980-49yv65gm 197 24 h h NN cord-279980-49yv65gm 197 25 postexposure postexposure NN cord-279980-49yv65gm 197 26 . . . cord-279980-49yv65gm 198 1 Otake Otake NNP cord-279980-49yv65gm 198 2 et et NNP cord-279980-49yv65gm 198 3 al al NNP cord-279980-49yv65gm 198 4 . . . cord-279980-49yv65gm 199 1 ( ( -LRB- cord-279980-49yv65gm 199 2 2003 2003 CD cord-279980-49yv65gm 199 3 ) ) -RRB- cord-279980-49yv65gm 199 4 showed show VBD cord-279980-49yv65gm 199 5 that that IN cord-279980-49yv65gm 199 6 the the DT cord-279980-49yv65gm 199 7 PRRSV PRRSV NNP cord-279980-49yv65gm 199 8 could could MD cord-279980-49yv65gm 199 9 survive survive VB cord-279980-49yv65gm 199 10 within within IN cord-279980-49yv65gm 199 11 the the DT cord-279980-49yv65gm 199 12 intestinal intestinal JJ cord-279980-49yv65gm 199 13 viscera viscera NN cord-279980-49yv65gm 199 14 of of IN cord-279980-49yv65gm 199 15 house house NN cord-279980-49yv65gm 199 16 flies fly VBZ cord-279980-49yv65gm 199 17 for for IN cord-279980-49yv65gm 199 18 up up IN cord-279980-49yv65gm 199 19 to to TO cord-279980-49yv65gm 199 20 12 12 CD cord-279980-49yv65gm 199 21 h h NN cord-279980-49yv65gm 199 22 post post NN cord-279980-49yv65gm 199 23 - - NN cord-279980-49yv65gm 199 24 exposure exposure NN cord-279980-49yv65gm 199 25 . . . cord-279980-49yv65gm 200 1 Moreover moreover RB cord-279980-49yv65gm 200 2 , , , cord-279980-49yv65gm 200 3 Watson Watson NNP cord-279980-49yv65gm 200 4 et et NNP cord-279980-49yv65gm 200 5 al al NNP cord-279980-49yv65gm 200 6 . . . cord-279980-49yv65gm 201 1 ( ( -LRB- cord-279980-49yv65gm 201 2 2007 2007 CD cord-279980-49yv65gm 201 3 ) ) -RRB- cord-279980-49yv65gm 201 4 found find VBD cord-279980-49yv65gm 201 5 that that IN cord-279980-49yv65gm 201 6 the the DT cord-279980-49yv65gm 201 7 NDV NDV NNP cord-279980-49yv65gm 201 8 remained remain VBD cord-279980-49yv65gm 201 9 viable viable JJ cord-279980-49yv65gm 201 10 in in IN cord-279980-49yv65gm 201 11 the the DT cord-279980-49yv65gm 201 12 crop crop NN cord-279980-49yv65gm 201 13 and and CC cord-279980-49yv65gm 201 14 gut gut NN cord-279980-49yv65gm 201 15 tissues tissue NNS cord-279980-49yv65gm 201 16 of of IN cord-279980-49yv65gm 201 17 the the DT cord-279980-49yv65gm 201 18 house house NN cord-279980-49yv65gm 201 19 flies fly VBZ cord-279980-49yv65gm 201 20 for for IN cord-279980-49yv65gm 201 21 up up IN cord-279980-49yv65gm 201 22 to to TO cord-279980-49yv65gm 201 23 96 96 CD cord-279980-49yv65gm 201 24 h h NN cord-279980-49yv65gm 201 25 post post NN cord-279980-49yv65gm 201 26 - - NN cord-279980-49yv65gm 201 27 exposure exposure NN cord-279980-49yv65gm 201 28 . . . cord-279980-49yv65gm 202 1 It -PRON- PRP cord-279980-49yv65gm 202 2 should should MD cord-279980-49yv65gm 202 3 be be VB cord-279980-49yv65gm 202 4 noted note VBN cord-279980-49yv65gm 202 5 that that IN cord-279980-49yv65gm 202 6 different different JJ cord-279980-49yv65gm 202 7 results result NNS cord-279980-49yv65gm 202 8 of of IN cord-279980-49yv65gm 202 9 viable viable JJ cord-279980-49yv65gm 202 10 virus virus NN cord-279980-49yv65gm 202 11 remaining remain VBG cord-279980-49yv65gm 202 12 in in IN cord-279980-49yv65gm 202 13 the the DT cord-279980-49yv65gm 202 14 digestive digestive JJ cord-279980-49yv65gm 202 15 tract tract NN cord-279980-49yv65gm 202 16 depend depend VBP cord-279980-49yv65gm 202 17 on on IN cord-279980-49yv65gm 202 18 the the DT cord-279980-49yv65gm 202 19 dimension dimension NN cord-279980-49yv65gm 202 20 of of IN cord-279980-49yv65gm 202 21 anatomy anatomy NN cord-279980-49yv65gm 202 22 , , , cord-279980-49yv65gm 202 23 the the DT cord-279980-49yv65gm 202 24 type type NN cord-279980-49yv65gm 202 25 of of IN cord-279980-49yv65gm 202 26 virus virus NN cord-279980-49yv65gm 202 27 and and CC cord-279980-49yv65gm 202 28 the the DT cord-279980-49yv65gm 202 29 route route NN cord-279980-49yv65gm 202 30 of of IN cord-279980-49yv65gm 202 31 transmission transmission NN cord-279980-49yv65gm 203 1 ( ( -LRB- cord-279980-49yv65gm 203 2 Förster Förster NNP cord-279980-49yv65gm 203 3 et et NNP cord-279980-49yv65gm 203 4 al al NNP cord-279980-49yv65gm 203 5 . . NNP cord-279980-49yv65gm 203 6 , , , cord-279980-49yv65gm 203 7 2007 2007 CD cord-279980-49yv65gm 203 8 ) ) -RRB- cord-279980-49yv65gm 203 9 . . . cord-279980-49yv65gm 204 1 However however RB cord-279980-49yv65gm 204 2 , , , cord-279980-49yv65gm 204 3 the the DT cord-279980-49yv65gm 204 4 real real JJ cord-279980-49yv65gm 204 5 - - HYPH cord-279980-49yv65gm 204 6 time time NN cord-279980-49yv65gm 204 7 RT RT NNP cord-279980-49yv65gm 204 8 - - HYPH cord-279980-49yv65gm 204 9 PCR PCR NNP cord-279980-49yv65gm 204 10 results result NNS cord-279980-49yv65gm 204 11 showed show VBD cord-279980-49yv65gm 204 12 that that IN cord-279980-49yv65gm 204 13 the the DT cord-279980-49yv65gm 204 14 viral viral JJ cord-279980-49yv65gm 204 15 RNA RNA NNP cord-279980-49yv65gm 204 16 can can MD cord-279980-49yv65gm 204 17 be be VB cord-279980-49yv65gm 204 18 found find VBN cord-279980-49yv65gm 204 19 in in IN cord-279980-49yv65gm 204 20 all all DT cord-279980-49yv65gm 204 21 time time NN cord-279980-49yv65gm 204 22 points point NNS cord-279980-49yv65gm 204 23 of of IN cord-279980-49yv65gm 204 24 this this DT cord-279980-49yv65gm 204 25 experiment experiment NN cord-279980-49yv65gm 204 26 . . . cord-279980-49yv65gm 205 1 In in IN cord-279980-49yv65gm 205 2 addition addition NN cord-279980-49yv65gm 205 3 , , , cord-279980-49yv65gm 205 4 the the DT cord-279980-49yv65gm 205 5 virus virus NN cord-279980-49yv65gm 205 6 infection infection NN cord-279980-49yv65gm 205 7 titre titre NN cord-279980-49yv65gm 205 8 continuously continuously RB cord-279980-49yv65gm 205 9 decreased decrease VBD cord-279980-49yv65gm 205 10 according accord VBG cord-279980-49yv65gm 205 11 to to IN cord-279980-49yv65gm 205 12 the the DT cord-279980-49yv65gm 205 13 increasing increase VBG cord-279980-49yv65gm 205 14 exposure exposure NN cord-279980-49yv65gm 205 15 time time NN cord-279980-49yv65gm 205 16 . . . cord-279980-49yv65gm 206 1 This this DT cord-279980-49yv65gm 206 2 study study NN cord-279980-49yv65gm 206 3 found find VBD cord-279980-49yv65gm 206 4 that that IN cord-279980-49yv65gm 206 5 the the DT cord-279980-49yv65gm 206 6 AI AI NNP cord-279980-49yv65gm 206 7 H5N1 H5N1 NNP cord-279980-49yv65gm 206 8 virus virus NN cord-279980-49yv65gm 206 9 could could MD cord-279980-49yv65gm 206 10 not not RB cord-279980-49yv65gm 206 11 replicate replicate VB cord-279980-49yv65gm 206 12 within within IN cord-279980-49yv65gm 206 13 the the DT cord-279980-49yv65gm 206 14 house house NN cord-279980-49yv65gm 206 15 flies fly VBZ cord-279980-49yv65gm 206 16 , , , cord-279980-49yv65gm 206 17 a a DT cord-279980-49yv65gm 206 18 mechanical mechanical JJ cord-279980-49yv65gm 206 19 vector vector NN cord-279980-49yv65gm 206 20 rather rather RB cord-279980-49yv65gm 206 21 than than IN cord-279980-49yv65gm 206 22 a a DT cord-279980-49yv65gm 206 23 biological biological JJ cord-279980-49yv65gm 206 24 vector vector NN cord-279980-49yv65gm 206 25 . . . cord-279980-49yv65gm 207 1 In in IN cord-279980-49yv65gm 207 2 conclusion conclusion NN cord-279980-49yv65gm 207 3 , , , cord-279980-49yv65gm 207 4 the the DT cord-279980-49yv65gm 207 5 present present JJ cord-279980-49yv65gm 207 6 study study NN cord-279980-49yv65gm 207 7 demonstrated demonstrate VBD cord-279980-49yv65gm 207 8 that that IN cord-279980-49yv65gm 207 9 the the DT cord-279980-49yv65gm 207 10 house house NN cord-279980-49yv65gm 207 11 flies fly NNS cord-279980-49yv65gm 207 12 could could MD cord-279980-49yv65gm 207 13 carry carry VB cord-279980-49yv65gm 207 14 the the DT cord-279980-49yv65gm 207 15 H5N1 H5N1 NNP cord-279980-49yv65gm 207 16 virus virus NN cord-279980-49yv65gm 207 17 , , , cord-279980-49yv65gm 207 18 which which WDT cord-279980-49yv65gm 207 19 remained remain VBD cord-279980-49yv65gm 207 20 infective infective JJ cord-279980-49yv65gm 207 21 for for IN cord-279980-49yv65gm 207 22 at at RB cord-279980-49yv65gm 207 23 least least RBS cord-279980-49yv65gm 207 24 72 72 CD cord-279980-49yv65gm 207 25 h. h. NN cord-279980-49yv65gm 208 1 The the DT cord-279980-49yv65gm 208 2 results result NNS cord-279980-49yv65gm 208 3 from from IN cord-279980-49yv65gm 208 4 the the DT cord-279980-49yv65gm 208 5 present present JJ cord-279980-49yv65gm 208 6 study study NN cord-279980-49yv65gm 208 7 indicated indicate VBD cord-279980-49yv65gm 208 8 that that IN cord-279980-49yv65gm 208 9 house house NN cord-279980-49yv65gm 208 10 flies fly NNS cord-279980-49yv65gm 208 11 may may MD cord-279980-49yv65gm 208 12 act act VB cord-279980-49yv65gm 208 13 as as IN cord-279980-49yv65gm 208 14 a a DT cord-279980-49yv65gm 208 15 potential potential JJ cord-279980-49yv65gm 208 16 mechanical mechanical JJ cord-279980-49yv65gm 208 17 vector vector NN cord-279980-49yv65gm 208 18 for for IN cord-279980-49yv65gm 208 19 spreading spread VBG cord-279980-49yv65gm 208 20 the the DT cord-279980-49yv65gm 208 21 AI AI NNP cord-279980-49yv65gm 208 22 H5N1 H5N1 NNP cord-279980-49yv65gm 208 23 virus virus NN cord-279980-49yv65gm 208 24 . . . cord-279980-49yv65gm 209 1 As as IN cord-279980-49yv65gm 209 2 a a DT cord-279980-49yv65gm 209 3 consequence consequence NN cord-279980-49yv65gm 209 4 , , , cord-279980-49yv65gm 209 5 improving improve VBG cord-279980-49yv65gm 209 6 biosecurity biosecurity NN cord-279980-49yv65gm 209 7 including include VBG cord-279980-49yv65gm 209 8 fly fly VB cord-279980-49yv65gm 209 9 management management NN cord-279980-49yv65gm 209 10 control control NN cord-279980-49yv65gm 209 11 should should MD cord-279980-49yv65gm 209 12 also also RB cord-279980-49yv65gm 209 13 be be VB cord-279980-49yv65gm 209 14 considered consider VBN cord-279980-49yv65gm 209 15 for for IN cord-279980-49yv65gm 209 16 the the DT cord-279980-49yv65gm 209 17 effective effective JJ cord-279980-49yv65gm 209 18 control control NN cord-279980-49yv65gm 209 19 of of IN cord-279980-49yv65gm 209 20 the the DT cord-279980-49yv65gm 209 21 AI AI NNP cord-279980-49yv65gm 209 22 H5N1 H5N1 NNP cord-279980-49yv65gm 209 23 virus virus NN cord-279980-49yv65gm 209 24 in in IN cord-279980-49yv65gm 209 25 epidemic epidemic NN cord-279980-49yv65gm 209 26 areas area NNS cord-279980-49yv65gm 209 27 . . . cord-279980-49yv65gm 210 1 However however RB cord-279980-49yv65gm 210 2 , , , cord-279980-49yv65gm 210 3 the the DT cord-279980-49yv65gm 210 4 actual actual JJ cord-279980-49yv65gm 210 5 ability ability NN cord-279980-49yv65gm 210 6 of of IN cord-279980-49yv65gm 210 7 infected infected JJ cord-279980-49yv65gm 210 8 house house NN cord-279980-49yv65gm 210 9 flies fly VBZ cord-279980-49yv65gm 210 10 in in IN cord-279980-49yv65gm 210 11 disseminating disseminate VBG cord-279980-49yv65gm 210 12 the the DT cord-279980-49yv65gm 210 13 AI AI NNP cord-279980-49yv65gm 210 14 H5N1 H5N1 NNP cord-279980-49yv65gm 210 15 virus virus NN cord-279980-49yv65gm 210 16 to to IN cord-279980-49yv65gm 210 17 the the DT cord-279980-49yv65gm 210 18 chicken chicken NN cord-279980-49yv65gm 210 19 has have VBZ cord-279980-49yv65gm 210 20 not not RB cord-279980-49yv65gm 210 21 been be VBN cord-279980-49yv65gm 210 22 determined determine VBN cord-279980-49yv65gm 210 23 in in IN cord-279980-49yv65gm 210 24 this this DT cord-279980-49yv65gm 210 25 study study NN cord-279980-49yv65gm 210 26 . . . cord-279980-49yv65gm 211 1 Therefore therefore RB cord-279980-49yv65gm 211 2 , , , cord-279980-49yv65gm 211 3 we -PRON- PRP cord-279980-49yv65gm 211 4 suggest suggest VBP cord-279980-49yv65gm 211 5 that that IN cord-279980-49yv65gm 211 6 the the DT cord-279980-49yv65gm 211 7 experimental experimental JJ cord-279980-49yv65gm 211 8 infection infection NN cord-279980-49yv65gm 211 9 with with IN cord-279980-49yv65gm 211 10 AI AI NNP cord-279980-49yv65gm 211 11 H5N1 H5N1 NNP cord-279980-49yv65gm 211 12 virus virus NN cord-279980-49yv65gm 211 13 exposed expose VBD cord-279980-49yv65gm 211 14 house house NN cord-279980-49yv65gm 211 15 flies fly NNS cord-279980-49yv65gm 211 16 in in IN cord-279980-49yv65gm 211 17 chickens chicken NNS cord-279980-49yv65gm 211 18 should should MD cord-279980-49yv65gm 211 19 be be VB cord-279980-49yv65gm 211 20 further further RB cord-279980-49yv65gm 211 21 performed perform VBN cord-279980-49yv65gm 211 22 . . . cord-279980-49yv65gm 212 1 A a DT cord-279980-49yv65gm 212 2 review review NN cord-279980-49yv65gm 212 3 of of IN cord-279980-49yv65gm 212 4 avian avian JJ cord-279980-49yv65gm 212 5 influenza influenza NN cord-279980-49yv65gm 212 6 in in IN cord-279980-49yv65gm 212 7 different different JJ cord-279980-49yv65gm 212 8 bird bird NN cord-279980-49yv65gm 212 9 species specie NNS cord-279980-49yv65gm 212 10 Competence competence NN cord-279980-49yv65gm 212 11 of of IN cord-279980-49yv65gm 212 12 the the DT cord-279980-49yv65gm 212 13 housefly housefly NN cord-279980-49yv65gm 212 14 , , , cord-279980-49yv65gm 212 15 Musca Musca NNP cord-279980-49yv65gm 212 16 domestica domestica NN cord-279980-49yv65gm 212 17 , , , cord-279980-49yv65gm 212 18 as as IN cord-279980-49yv65gm 212 19 a a DT cord-279980-49yv65gm 212 20 vector vector NN cord-279980-49yv65gm 212 21 of of IN cord-279980-49yv65gm 212 22 Microsporum Microsporum NNP cord-279980-49yv65gm 212 23 canis canis NN cord-279980-49yv65gm 212 24 under under IN cord-279980-49yv65gm 212 25 experimental experimental JJ cord-279980-49yv65gm 212 26 conditions condition NNS cord-279980-49yv65gm 212 27 Mechanical mechanical JJ cord-279980-49yv65gm 212 28 transmission transmission NN cord-279980-49yv65gm 212 29 of of IN cord-279980-49yv65gm 212 30 turkey turkey NN cord-279980-49yv65gm 212 31 coronavirus coronavirus NN cord-279980-49yv65gm 212 32 by by IN cord-279980-49yv65gm 212 33 domestic domestic JJ cord-279980-49yv65gm 212 34 houseflies housefly NNS cord-279980-49yv65gm 212 35 ( ( -LRB- cord-279980-49yv65gm 212 36 Musca Musca NNP cord-279980-49yv65gm 212 37 domestica domestica NNP cord-279980-49yv65gm 212 38 Linnaeaus Linnaeaus NNP cord-279980-49yv65gm 212 39 ) ) -RRB- cord-279980-49yv65gm 212 40 Persistence Persistence NNP cord-279980-49yv65gm 212 41 of of IN cord-279980-49yv65gm 212 42 exotic exotic JJ cord-279980-49yv65gm 212 43 newcastle newcastle NNP cord-279980-49yv65gm 212 44 disease disease NNP cord-279980-49yv65gm 212 45 virus virus NN cord-279980-49yv65gm 212 46 ( ( -LRB- cord-279980-49yv65gm 212 47 ENDV ENDV NNP cord-279980-49yv65gm 212 48 ) ) -RRB- cord-279980-49yv65gm 212 49 in in IN cord-279980-49yv65gm 212 50 laboratory laboratory NN cord-279980-49yv65gm 212 51 infected infect VBN cord-279980-49yv65gm 212 52 Musca Musca NNP cord-279980-49yv65gm 212 53 domestica domestica NN cord-279980-49yv65gm 212 54 and and CC cord-279980-49yv65gm 212 55 Fannia Fannia NNP cord-279980-49yv65gm 212 56 canicularis canicularis NNP cord-279980-49yv65gm 213 1 The the DT cord-279980-49yv65gm 213 2 evolution evolution NN cord-279980-49yv65gm 213 3 of of IN cord-279980-49yv65gm 213 4 H5N1 H5N1 NNP cord-279980-49yv65gm 213 5 influenza influenza NN cord-279980-49yv65gm 213 6 viruses virus NNS cord-279980-49yv65gm 213 7 in in IN cord-279980-49yv65gm 213 8 ducks duck NNS cord-279980-49yv65gm 213 9 in in IN cord-279980-49yv65gm 213 10 southern southern JJ cord-279980-49yv65gm 213 11 China China NNP cord-279980-49yv65gm 213 12 . . . cord-279980-49yv65gm 214 1 Proceeding proceed VBG cord-279980-49yv65gm 214 2 of of IN cord-279980-49yv65gm 214 3 the the DT cord-279980-49yv65gm 214 4 National National NNP cord-279980-49yv65gm 214 5 Academy Academy NNP cord-279980-49yv65gm 214 6 of of IN cord-279980-49yv65gm 214 7 Sciences Sciences NNPS cord-279980-49yv65gm 214 8 of of IN cord-279980-49yv65gm 214 9 the the DT cord-279980-49yv65gm 214 10 United United NNP cord-279980-49yv65gm 214 11 States States NNP cord-279980-49yv65gm 214 12 America America NNP cord-279980-49yv65gm 215 1 The the DT cord-279980-49yv65gm 215 2 effect effect NN cord-279980-49yv65gm 215 3 of of IN cord-279980-49yv65gm 215 4 temperature temperature NN cord-279980-49yv65gm 215 5 and and CC cord-279980-49yv65gm 215 6 UV uv NN cord-279980-49yv65gm 215 7 light light NN cord-279980-49yv65gm 215 8 on on IN cord-279980-49yv65gm 215 9 infectivity infectivity NN cord-279980-49yv65gm 215 10 of of IN cord-279980-49yv65gm 215 11 avian avian JJ cord-279980-49yv65gm 215 12 influenza influenza NN cord-279980-49yv65gm 215 13 virus virus NN cord-279980-49yv65gm 215 14 ( ( -LRB- cord-279980-49yv65gm 215 15 H5N1 H5N1 NNP cord-279980-49yv65gm 215 16 , , , cord-279980-49yv65gm 215 17 Thai Thai NNP cord-279980-49yv65gm 215 18 field field NN cord-279980-49yv65gm 215 19 strain strain NN cord-279980-49yv65gm 215 20 ) ) -RRB- cord-279980-49yv65gm 215 21 in in IN cord-279980-49yv65gm 215 22 chicken chicken NN cord-279980-49yv65gm 215 23 fecal fecal JJ cord-279980-49yv65gm 215 24 manure manure NN cord-279980-49yv65gm 215 25 Quantitative quantitative JJ cord-279980-49yv65gm 215 26 contamination contamination NN cord-279980-49yv65gm 215 27 and and CC cord-279980-49yv65gm 215 28 transfer transfer NN cord-279980-49yv65gm 215 29 of of IN cord-279980-49yv65gm 215 30 Escherichia Escherichia NNP cord-279980-49yv65gm 215 31 coli coli NNS cord-279980-49yv65gm 215 32 from from IN cord-279980-49yv65gm 215 33 foods food NNS cord-279980-49yv65gm 215 34 by by IN cord-279980-49yv65gm 215 35 houseflies housefly NNS cord-279980-49yv65gm 215 36 , , , cord-279980-49yv65gm 215 37 Musca Musca NNP cord-279980-49yv65gm 215 38 domestica domestica NN cord-279980-49yv65gm 215 39 L. L. NNP cord-279980-49yv65gm 216 1 ( ( -LRB- cord-279980-49yv65gm 216 2 Diptera Diptera NNP cord-279980-49yv65gm 216 3 : : : cord-279980-49yv65gm 216 4 Muscidae Muscidae NNP cord-279980-49yv65gm 216 5 ) ) -RRB- cord-279980-49yv65gm 216 6 Functional Functional NNP cord-279980-49yv65gm 216 7 association association NN cord-279980-49yv65gm 216 8 between between IN cord-279980-49yv65gm 216 9 viral viral JJ cord-279980-49yv65gm 216 10 and and CC cord-279980-49yv65gm 216 11 cellular cellular JJ cord-279980-49yv65gm 216 12 transcription transcription NN cord-279980-49yv65gm 216 13 during during IN cord-279980-49yv65gm 216 14 influenza influenza NN cord-279980-49yv65gm 216 15 virus virus NN cord-279980-49yv65gm 216 16 infection infection NN cord-279980-49yv65gm 216 17 Pilot Pilot NNP cord-279980-49yv65gm 216 18 study study NN cord-279980-49yv65gm 216 19 on on IN cord-279980-49yv65gm 216 20 synanthropic synanthropic NNP cord-279980-49yv65gm 216 21 flies fly NNS cord-279980-49yv65gm 216 22 ( ( -LRB- cord-279980-49yv65gm 216 23 e.g. e.g. IN cord-279980-49yv65gm 216 24 Musca Musca NNP cord-279980-49yv65gm 216 25 , , , cord-279980-49yv65gm 216 26 Sarcophaga Sarcophaga NNP cord-279980-49yv65gm 216 27 , , , cord-279980-49yv65gm 216 28 Calliphora Calliphora NNP cord-279980-49yv65gm 216 29 , , , cord-279980-49yv65gm 216 30 Fannia Fannia NNP cord-279980-49yv65gm 216 31 , , , cord-279980-49yv65gm 216 32 Lucilia Lucilia NNP cord-279980-49yv65gm 216 33 , , , cord-279980-49yv65gm 216 34 Stomoxys Stomoxys NNP cord-279980-49yv65gm 216 35 ) ) -RRB- cord-279980-49yv65gm 216 36 as as IN cord-279980-49yv65gm 216 37 vectors vector NNS cord-279980-49yv65gm 216 38 of of IN cord-279980-49yv65gm 216 39 pathogenic pathogenic JJ cord-279980-49yv65gm 216 40 microorganisms microorganism NNS cord-279980-49yv65gm 216 41 Characterization characterization NN cord-279980-49yv65gm 216 42 of of IN cord-279980-49yv65gm 216 43 a a DT cord-279980-49yv65gm 216 44 novel novel JJ cord-279980-49yv65gm 216 45 influenza influenza NN cord-279980-49yv65gm 216 46 A a DT cord-279980-49yv65gm 216 47 virus virus NN cord-279980-49yv65gm 216 48 hemagglutinin hemagglutinin NN cord-279980-49yv65gm 216 49 subtype subtype NN cord-279980-49yv65gm 216 50 ( ( -LRB- cord-279980-49yv65gm 216 51 H16 H16 NNP cord-279980-49yv65gm 216 52 ) ) -RRB- cord-279980-49yv65gm 216 53 obtained obtain VBN cord-279980-49yv65gm 216 54 from from IN cord-279980-49yv65gm 216 55 black black JJ cord-279980-49yv65gm 216 56 - - HYPH cord-279980-49yv65gm 216 57 headed head VBN cord-279980-49yv65gm 216 58 gulls gull NNS cord-279980-49yv65gm 216 59 Flies Flies NNPS cord-279980-49yv65gm 216 60 and and CC cord-279980-49yv65gm 216 61 Disease Disease NNP cord-279980-49yv65gm 217 1 The the DT cord-279980-49yv65gm 217 2 House House NNP cord-279980-49yv65gm 217 3 Fly Fly NNP cord-279980-49yv65gm 217 4 and and CC cord-279980-49yv65gm 217 5 its -PRON- PRP$ cord-279980-49yv65gm 217 6 Relatives relative NNS cord-279980-49yv65gm 217 7 , , , cord-279980-49yv65gm 217 8 Herms Herms NNPS cord-279980-49yv65gm 217 9 's 's POS cord-279980-49yv65gm 217 10 Medical medical JJ cord-279980-49yv65gm 217 11 Entomology Entomology NNP cord-279980-49yv65gm 217 12 Isolation Isolation NNP cord-279980-49yv65gm 217 13 of of IN cord-279980-49yv65gm 217 14 Salmonella Salmonella NNP cord-279980-49yv65gm 217 15 enterica enterica NNP cord-279980-49yv65gm 217 16 serovar serovar NNP cord-279980-49yv65gm 217 17 Enteritidis Enteritidis NNP cord-279980-49yv65gm 217 18 from from IN cord-279980-49yv65gm 217 19 houseflies housefly NNS cord-279980-49yv65gm 217 20 ( ( -LRB- cord-279980-49yv65gm 217 21 Musca Musca NNP cord-279980-49yv65gm 217 22 domestica domestica NN cord-279980-49yv65gm 217 23 ) ) -RRB- cord-279980-49yv65gm 217 24 found find VBN cord-279980-49yv65gm 217 25 in in IN cord-279980-49yv65gm 217 26 rooms room NNS cord-279980-49yv65gm 217 27 containing contain VBG cord-279980-49yv65gm 217 28 Salmonella Salmonella NNP cord-279980-49yv65gm 217 29 serovar serovar NNP cord-279980-49yv65gm 217 30 Enteritidis Enteritidis NNP cord-279980-49yv65gm 217 31 - - HYPH cord-279980-49yv65gm 217 32 challenged challenge VBN cord-279980-49yv65gm 217 33 hens hen NNS cord-279980-49yv65gm 217 34 Experimental experimental JJ cord-279980-49yv65gm 217 35 infection infection NN cord-279980-49yv65gm 217 36 of of IN cord-279980-49yv65gm 217 37 chickens chicken NNS cord-279980-49yv65gm 217 38 , , , cord-279980-49yv65gm 217 39 ducks duck NNS cord-279980-49yv65gm 217 40 and and CC cord-279980-49yv65gm 217 41 quails quail NNS cord-279980-49yv65gm 217 42 with with IN cord-279980-49yv65gm 217 43 the the DT cord-279980-49yv65gm 217 44 highly highly RB cord-279980-49yv65gm 217 45 pathogenic pathogenic JJ cord-279980-49yv65gm 217 46 H5N1 H5N1 NNP cord-279980-49yv65gm 217 47 avian avian JJ cord-279980-49yv65gm 217 48 influenza influenza NN cord-279980-49yv65gm 217 49 virus virus NN cord-279980-49yv65gm 217 50 Movement movement NN cord-279980-49yv65gm 217 51 and and CC cord-279980-49yv65gm 217 52 distribution distribution NN cord-279980-49yv65gm 217 53 of of IN cord-279980-49yv65gm 217 54 house house NN cord-279980-49yv65gm 217 55 flies fly NNS cord-279980-49yv65gm 218 1 ( ( -LRB- cord-279980-49yv65gm 218 2 Diptera Diptera NNP cord-279980-49yv65gm 218 3 : : : cord-279980-49yv65gm 218 4 Muscidae Muscidae NNP cord-279980-49yv65gm 218 5 ) ) -RRB- cord-279980-49yv65gm 218 6 between between IN cord-279980-49yv65gm 218 7 habitats habitat NNS cord-279980-49yv65gm 218 8 in in IN cord-279980-49yv65gm 218 9 two two CD cord-279980-49yv65gm 218 10 livestock livestock NN cord-279980-49yv65gm 218 11 farms farm NNS cord-279980-49yv65gm 219 1 Vector vector NN cord-279980-49yv65gm 219 2 potential potential NN cord-279980-49yv65gm 219 3 of of IN cord-279980-49yv65gm 219 4 houseflies housefly NNS cord-279980-49yv65gm 219 5 for for IN cord-279980-49yv65gm 219 6 the the DT cord-279980-49yv65gm 219 7 bacterium bacterium NN cord-279980-49yv65gm 219 8 Aeromonas Aeromonas NNP cord-279980-49yv65gm 219 9 caviae caviae NNP cord-279980-49yv65gm 220 1 Influenza Influenza NNP cord-279980-49yv65gm 220 2 , , , cord-279980-49yv65gm 220 3 Manual Manual NNP cord-279980-49yv65gm 220 4 of of IN cord-279980-49yv65gm 220 5 Standards Standards NNPS cord-279980-49yv65gm 220 6 for for IN cord-279980-49yv65gm 220 7 Diagnostic Diagnostic NNP cord-279980-49yv65gm 220 8 Tests Tests NNPS cord-279980-49yv65gm 220 9 and and CC cord-279980-49yv65gm 221 1 Vaccines Vaccines NNP cord-279980-49yv65gm 221 2 Office Office NNP cord-279980-49yv65gm 221 3 International International NNP cord-279980-49yv65gm 221 4 des des FW cord-279980-49yv65gm 221 5 Epizooties Epizooties NNP cord-279980-49yv65gm 221 6 ( ( -LRB- cord-279980-49yv65gm 221 7 OIE OIE NNP cord-279980-49yv65gm 221 8 ) ) -RRB- cord-279980-49yv65gm 221 9 ( ( -LRB- cord-279980-49yv65gm 221 10 2008 2008 CD cord-279980-49yv65gm 221 11 ) ) -RRB- cord-279980-49yv65gm 222 1 Update update VB cord-279980-49yv65gm 222 2 on on IN cord-279980-49yv65gm 222 3 Highly highly RB cord-279980-49yv65gm 222 4 Pathogenic pathogenic JJ cord-279980-49yv65gm 222 5 Avian avian JJ cord-279980-49yv65gm 222 6 Influenza Influenza NNP cord-279980-49yv65gm 222 7 in in IN cord-279980-49yv65gm 222 8 Animals animal NNS cord-279980-49yv65gm 222 9 ( ( -LRB- cord-279980-49yv65gm 222 10 type type NN cord-279980-49yv65gm 222 11 H5 H5 NNP cord-279980-49yv65gm 222 12 and and CC cord-279980-49yv65gm 222 13 H7 H7 NNP cord-279980-49yv65gm 222 14 Survival Survival NNP cord-279980-49yv65gm 222 15 of of IN cord-279980-49yv65gm 222 16 porcine porcine JJ cord-279980-49yv65gm 222 17 reproductive reproductive JJ cord-279980-49yv65gm 222 18 and and CC cord-279980-49yv65gm 222 19 respiratory respiratory JJ cord-279980-49yv65gm 222 20 syndrome syndrome NN cord-279980-49yv65gm 222 21 virus virus NN cord-279980-49yv65gm 222 22 in in IN cord-279980-49yv65gm 222 23 houseflies housefly NNS cord-279980-49yv65gm 222 24 Pathogenesis Pathogenesis NNP cord-279980-49yv65gm 222 25 and and CC cord-279980-49yv65gm 222 26 pathobiology pathobiology NN cord-279980-49yv65gm 222 27 of of IN cord-279980-49yv65gm 222 28 avian avian JJ cord-279980-49yv65gm 222 29 influenza influenza NN cord-279980-49yv65gm 222 30 virus virus NN cord-279980-49yv65gm 222 31 infection infection NN cord-279980-49yv65gm 222 32 in in IN cord-279980-49yv65gm 222 33 birds bird NNS cord-279980-49yv65gm 223 1 Single single JJ cord-279980-49yv65gm 223 2 step step NN cord-279980-49yv65gm 223 3 multiplex multiplex NN cord-279980-49yv65gm 223 4 real real JJ cord-279980-49yv65gm 223 5 - - HYPH cord-279980-49yv65gm 223 6 time time NN cord-279980-49yv65gm 223 7 RT rt NN cord-279980-49yv65gm 223 8 - - HYPH cord-279980-49yv65gm 223 9 PCR PCR NNP cord-279980-49yv65gm 223 10 for for IN cord-279980-49yv65gm 223 11 H5N1 H5N1 NNP cord-279980-49yv65gm 224 1 influenza influenza NN cord-279980-49yv65gm 224 2 A a DT cord-279980-49yv65gm 224 3 virus virus NN cord-279980-49yv65gm 224 4 detection detection NN cord-279980-49yv65gm 225 1 A a DT cord-279980-49yv65gm 225 2 simple simple JJ cord-279980-49yv65gm 225 3 method method NN cord-279980-49yv65gm 225 4 of of IN cord-279980-49yv65gm 225 5 estimating estimate VBG cord-279980-49yv65gm 225 6 fifty fifty CD cord-279980-49yv65gm 225 7 percent percent NN cord-279980-49yv65gm 225 8 endpoints endpoint NNS cord-279980-49yv65gm 225 9 Survival Survival NNP cord-279980-49yv65gm 225 10 of of IN cord-279980-49yv65gm 225 11 avian avian JJ cord-279980-49yv65gm 225 12 H5N1 H5N1 NNP cord-279980-49yv65gm 225 13 influenza influenza NN cord-279980-49yv65gm 225 14 A a DT cord-279980-49yv65gm 225 15 viruses virus NNS cord-279980-49yv65gm 225 16 in in IN cord-279980-49yv65gm 225 17 Calliphora Calliphora NNP cord-279980-49yv65gm 225 18 nigribarbis nigribarbis NNP cord-279980-49yv65gm 226 1 ( ( -LRB- cord-279980-49yv65gm 226 2 Diptera Diptera NNS cord-279980-49yv65gm 226 3 : : : cord-279980-49yv65gm 226 4 Calliphoridae Calliphoridae NNP cord-279980-49yv65gm 226 5 ) ) -RRB- cord-279980-49yv65gm 226 6 Origin origin NN cord-279980-49yv65gm 226 7 and and CC cord-279980-49yv65gm 226 8 evolution evolution NN cord-279980-49yv65gm 226 9 of of IN cord-279980-49yv65gm 226 10 highly highly RB cord-279980-49yv65gm 226 11 pathogenic pathogenic JJ cord-279980-49yv65gm 226 12 H5N1 H5N1 NNP cord-279980-49yv65gm 226 13 avian avian JJ cord-279980-49yv65gm 226 14 influenza influenza NN cord-279980-49yv65gm 226 15 in in IN cord-279980-49yv65gm 226 16 Asia Asia NNP cord-279980-49yv65gm 226 17 Development Development NNP cord-279980-49yv65gm 226 18 of of IN cord-279980-49yv65gm 226 19 a a DT cord-279980-49yv65gm 226 20 real real JJ cord-279980-49yv65gm 226 21 - - HYPH cord-279980-49yv65gm 226 22 time time NN cord-279980-49yv65gm 226 23 reverse reverse NN cord-279980-49yv65gm 226 24 transcriptase transcriptase NN cord-279980-49yv65gm 226 25 PCR pcr NN cord-279980-49yv65gm 226 26 assay assay NN cord-279980-49yv65gm 226 27 for for IN cord-279980-49yv65gm 226 28 type type NN cord-279980-49yv65gm 226 29 A a DT cord-279980-49yv65gm 226 30 influenza influenza NN cord-279980-49yv65gm 226 31 virus virus NN cord-279980-49yv65gm 226 32 and and CC cord-279980-49yv65gm 226 33 the the DT cord-279980-49yv65gm 226 34 avian avian JJ cord-279980-49yv65gm 226 35 H5 H5 NNP cord-279980-49yv65gm 226 36 and and CC cord-279980-49yv65gm 226 37 H7 H7 NNP cord-279980-49yv65gm 226 38 hemagglutinin hemagglutinin NN cord-279980-49yv65gm 226 39 subtypes subtype NNS cord-279980-49yv65gm 227 1 Influenza Influenza NNP cord-279980-49yv65gm 227 2 , , , cord-279980-49yv65gm 227 3 Diseases Diseases NNPS cord-279980-49yv65gm 227 4 of of IN cord-279980-49yv65gm 227 5 Poultry Poultry NNP cord-279980-49yv65gm 227 6 Mechanical Mechanical NNP cord-279980-49yv65gm 227 7 transport transport NN cord-279980-49yv65gm 227 8 of of IN cord-279980-49yv65gm 227 9 rotavirus rotavirus NN cord-279980-49yv65gm 227 10 by by IN cord-279980-49yv65gm 227 11 the the DT cord-279980-49yv65gm 227 12 legs leg NNS cord-279980-49yv65gm 227 13 and and CC cord-279980-49yv65gm 227 14 wings wing NNS cord-279980-49yv65gm 227 15 of of IN cord-279980-49yv65gm 227 16 Musca Musca NNP cord-279980-49yv65gm 227 17 domestica domestica NN cord-279980-49yv65gm 227 18 ( ( -LRB- cord-279980-49yv65gm 227 19 Diptera Diptera NNP cord-279980-49yv65gm 227 20 : : : cord-279980-49yv65gm 227 21 Muscidae Muscidae NNP cord-279980-49yv65gm 227 22 ) ) -RRB- cord-279980-49yv65gm 227 23 Transmission transmission NN cord-279980-49yv65gm 227 24 of of IN cord-279980-49yv65gm 227 25 the the DT cord-279980-49yv65gm 227 26 highly highly RB cord-279980-49yv65gm 227 27 pathogenic pathogenic JJ cord-279980-49yv65gm 227 28 avian avian JJ cord-279980-49yv65gm 227 29 influenza influenza NN cord-279980-49yv65gm 227 30 A a DT cord-279980-49yv65gm 227 31 virus virus NN cord-279980-49yv65gm 227 32 ( ( -LRB- cord-279980-49yv65gm 227 33 H5N1 H5N1 NNP cord-279980-49yv65gm 227 34 ) ) -RRB- cord-279980-49yv65gm 227 35 in in IN cord-279980-49yv65gm 227 36 Thailand Thailand NNP cord-279980-49yv65gm 227 37 Experimental experimental JJ cord-279980-49yv65gm 227 38 evaluation evaluation NN cord-279980-49yv65gm 227 39 of of IN cord-279980-49yv65gm 227 40 Musca Musca NNP cord-279980-49yv65gm 227 41 domestica domestica NN cord-279980-49yv65gm 227 42 ( ( -LRB- cord-279980-49yv65gm 227 43 Diptera Diptera NNP cord-279980-49yv65gm 227 44 : : : cord-279980-49yv65gm 227 45 Muscidae Muscidae NNP cord-279980-49yv65gm 227 46 ) ) -RRB- cord-279980-49yv65gm 227 47 as as IN cord-279980-49yv65gm 227 48 a a DT cord-279980-49yv65gm 227 49 vector vector NN cord-279980-49yv65gm 227 50 of of IN cord-279980-49yv65gm 227 51 Newcastle Newcastle NNP cord-279980-49yv65gm 227 52 disease disease NN cord-279980-49yv65gm 227 53 virus virus NN cord-279980-49yv65gm 227 54 Cumulative Cumulative NNP cord-279980-49yv65gm 227 55 Number Number NNP cord-279980-49yv65gm 227 56 of of IN cord-279980-49yv65gm 227 57 Confirmed confirm VBN cord-279980-49yv65gm 227 58 Human Human NNP cord-279980-49yv65gm 227 59 Cases Cases NNPS cord-279980-49yv65gm 227 60 of of IN cord-279980-49yv65gm 227 61 Avian avian JJ cord-279980-49yv65gm 227 62 Influenza Influenza NNP cord-279980-49yv65gm 227 63 A/(H5N1 A/(H5N1 NNP cord-279980-49yv65gm 227 64 ) ) -RRB- cord-279980-49yv65gm 228 1 Reported report VBN cord-279980-49yv65gm 228 2 to to IN cord-279980-49yv65gm 228 3 WHO who WP cord-279980-49yv65gm 228 4 ) ) -RRB- cord-279980-49yv65gm 229 1 Wings wing NNS cord-279980-49yv65gm 229 2 of of IN cord-279980-49yv65gm 229 3 the the DT cord-279980-49yv65gm 229 4 common common JJ cord-279980-49yv65gm 229 5 house house NNP cord-279980-49yv65gm 229 6 fly fly NN cord-279980-49yv65gm 229 7 ( ( -LRB- cord-279980-49yv65gm 229 8 Musca Musca NNP cord-279980-49yv65gm 229 9 domestica domestica NNP cord-279980-49yv65gm 229 10 L. L. NNP cord-279980-49yv65gm 229 11 ) ) -RRB- cord-279980-49yv65gm 229 12 : : : cord-279980-49yv65gm 229 13 importance importance NN cord-279980-49yv65gm 229 14 in in IN cord-279980-49yv65gm 229 15 mechanical mechanical JJ cord-279980-49yv65gm 229 16 transmission transmission NN cord-279980-49yv65gm 229 17 of of IN cord-279980-49yv65gm 229 18 Vibrio Vibrio NNP cord-279980-49yv65gm 229 19 cholerae cholerae NN cord-279980-49yv65gm 230 1 The the DT cord-279980-49yv65gm 230 2 authors author NNS cord-279980-49yv65gm 230 3 are be VBP cord-279980-49yv65gm 230 4 grateful grateful JJ cord-279980-49yv65gm 230 5 to to IN cord-279980-49yv65gm 230 6 Dr Dr NNP cord-279980-49yv65gm 230 7 Nareerat Nareerat NNP cord-279980-49yv65gm 230 8 Viseshakul Viseshakul NNP cord-279980-49yv65gm 230 9 , , , cord-279980-49yv65gm 230 10 Dr Dr NNP cord-279980-49yv65gm 230 11 Jiroj Jiroj NNP cord-279980-49yv65gm 230 12 Sasipreeyajan Sasipreeyajan NNP cord-279980-49yv65gm 230 13 , , , cord-279980-49yv65gm 230 14 Dr Dr NNP cord-279980-49yv65gm 230 15 Padet Padet NNP cord-279980-49yv65gm 230 16 Tammaruk Tammaruk NNP cord-279980-49yv65gm 230 17 , , , cord-279980-49yv65gm 230 18 the the DT cord-279980-49yv65gm 230 19 Virology Virology NNP cord-279980-49yv65gm 230 20 Unit Unit NNP cord-279980-49yv65gm 230 21 , , , cord-279980-49yv65gm 230 22 the the DT cord-279980-49yv65gm 230 23 Veterinary Veterinary NNP cord-279980-49yv65gm 230 24 Diagnostic Diagnostic NNP cord-279980-49yv65gm 230 25 Laboratory Laboratory NNP cord-279980-49yv65gm 230 26 , , , cord-279980-49yv65gm 230 27 Faculty Faculty NNP cord-279980-49yv65gm 230 28 of of IN cord-279980-49yv65gm 230 29 Veterinary Veterinary NNP cord-279980-49yv65gm 230 30 Science Science NNP cord-279980-49yv65gm 230 31 , , , cord-279980-49yv65gm 230 32 Chulalongkorn Chulalongkorn NNP cord-279980-49yv65gm 230 33 University University NNP cord-279980-49yv65gm 230 34 for for IN cord-279980-49yv65gm 230 35 the the DT cord-279980-49yv65gm 230 36 laboratory laboratory NN cord-279980-49yv65gm 230 37 and and CC cord-279980-49yv65gm 230 38 technical technical JJ cord-279980-49yv65gm 230 39 assistance assistance NN cord-279980-49yv65gm 230 40 . . . cord-279980-49yv65gm 231 1 This this DT cord-279980-49yv65gm 231 2 research research NN cord-279980-49yv65gm 231 3 was be VBD cord-279980-49yv65gm 231 4 supported support VBN cord-279980-49yv65gm 231 5 by by IN cord-279980-49yv65gm 231 6 a a DT cord-279980-49yv65gm 231 7 grant grant NN cord-279980-49yv65gm 231 8 from from IN cord-279980-49yv65gm 231 9 the the DT cord-279980-49yv65gm 231 10 program program NN cord-279980-49yv65gm 231 11 Strategic Strategic NNP cord-279980-49yv65gm 231 12 Scholarships Scholarships NNPS cord-279980-49yv65gm 231 13 for for IN cord-279980-49yv65gm 231 14 Frontier Frontier NNP cord-279980-49yv65gm 231 15 Research Research NNP cord-279980-49yv65gm 231 16 Network Network NNP cord-279980-49yv65gm 231 17 for for IN cord-279980-49yv65gm 231 18 the the DT cord-279980-49yv65gm 231 19 Joint Joint NNP cord-279980-49yv65gm 231 20 PhD phd NN cord-279980-49yv65gm 231 21 Program Program NNP cord-279980-49yv65gm 231 22 Thai Thai NNP cord-279980-49yv65gm 231 23 Doctoral Doctoral NNP cord-279980-49yv65gm 231 24 degree degree NN cord-279980-49yv65gm 231 25 from from IN cord-279980-49yv65gm 231 26 the the DT cord-279980-49yv65gm 231 27 Office Office NNP cord-279980-49yv65gm 231 28 of of IN cord-279980-49yv65gm 231 29 the the DT cord-279980-49yv65gm 231 30 Higher Higher NNP cord-279980-49yv65gm 231 31 Education Education NNP cord-279980-49yv65gm 231 32 Commission Commission NNP cord-279980-49yv65gm 231 33 , , , cord-279980-49yv65gm 231 34 Thailand Thailand NNP cord-279980-49yv65gm 231 35 and and CC cord-279980-49yv65gm 231 36 the the DT cord-279980-49yv65gm 231 37 90 90 CD cord-279980-49yv65gm 231 38 th th XX cord-279980-49yv65gm 231 39 anniversary anniversary NN cord-279980-49yv65gm 231 40 of of IN cord-279980-49yv65gm 231 41 the the DT cord-279980-49yv65gm 231 42 Chulalongkorn Chulalongkorn NNP cord-279980-49yv65gm 231 43 University University NNP cord-279980-49yv65gm 231 44 Fund Fund NNP cord-279980-49yv65gm 231 45 ( ( -LRB- cord-279980-49yv65gm 231 46 Ratchadaphiseksomphot Ratchadaphiseksomphot NNP cord-279980-49yv65gm 231 47 Endowment Endowment NNP cord-279980-49yv65gm 231 48 Fund Fund NNP cord-279980-49yv65gm 231 49 ) ) -RRB- cord-279980-49yv65gm 231 50 . . . cord-279980-49yv65gm 232 1 Potential potential NN cord-279980-49yv65gm 232 2 of of IN cord-279980-49yv65gm 232 3 house house NN cord-279980-49yv65gm 232 4 flies fly NNS cord-279980-49yv65gm 232 5 as as IN cord-279980-49yv65gm 232 6 a a DT cord-279980-49yv65gm 232 7 vector vector NN cord-279980-49yv65gm 232 8 of of IN cord-279980-49yv65gm 232 9 AI AI NNP cord-279980-49yv65gm 232 10 H5N1 H5N1 NNP cord-279980-49yv65gm 232 11 63 63 CD