id	sid	tid	token	lemma	pos
cord-276978-xl4u7n6r	1	1	key	key	NN
cord-276978-xl4u7n6r	1	2	:	:	:
cord-276978-xl4u7n6r	2	1	cord-276978-xl4u7n6r	cord-276978-xl4u7n6r	JJ
cord-276978-xl4u7n6r	2	2	authors	author	NNS
cord-276978-xl4u7n6r	2	3	:	:	:
cord-276978-xl4u7n6r	2	4	Jonassen	Jonassen	NNP
cord-276978-xl4u7n6r	2	5	,	,	,
cord-276978-xl4u7n6r	2	6	Christine	Christine	NNP
cord-276978-xl4u7n6r	2	7	Monceyron	Monceyron	NNP
cord-276978-xl4u7n6r	2	8	title	title	NN
cord-276978-xl4u7n6r	2	9	:	:	:
cord-276978-xl4u7n6r	2	10	Detection	detection	NN
cord-276978-xl4u7n6r	2	11	and	and	CC
cord-276978-xl4u7n6r	2	12	Sequence	Sequence	NNP
cord-276978-xl4u7n6r	2	13	Characterization	characterization	NN
cord-276978-xl4u7n6r	2	14	of	of	IN
cord-276978-xl4u7n6r	2	15	the	the	DT
cord-276978-xl4u7n6r	2	16	3′-End	3′-end	CD
cord-276978-xl4u7n6r	2	17	of	of	IN
cord-276978-xl4u7n6r	2	18	Coronavirus	Coronavirus	NNP
cord-276978-xl4u7n6r	2	19	Genomes	Genomes	NNPS
cord-276978-xl4u7n6r	2	20	Harboring	harbor	VBG
cord-276978-xl4u7n6r	2	21	the	the	DT
cord-276978-xl4u7n6r	2	22	Highly	highly	RB
cord-276978-xl4u7n6r	2	23	Conserved	conserve	VBN
cord-276978-xl4u7n6r	2	24	RNA	RNA	NNP
cord-276978-xl4u7n6r	2	25	Motif	motif	NN
cord-276978-xl4u7n6r	2	26	s2	s2	NN
cord-276978-xl4u7n6r	2	27	m	m	CD
cord-276978-xl4u7n6r	2	28	date	date	NN
cord-276978-xl4u7n6r	2	29	:	:	:
cord-276978-xl4u7n6r	2	30	2007	2007	CD
cord-276978-xl4u7n6r	2	31	-	-	SYM
cord-276978-xl4u7n6r	2	32	11	11	CD
cord-276978-xl4u7n6r	2	33	-	-	HYPH
cord-276978-xl4u7n6r	2	34	28	28	CD
cord-276978-xl4u7n6r	2	35	journal	journal	NN
cord-276978-xl4u7n6r	2	36	:	:	:
cord-276978-xl4u7n6r	2	37	SARS-	SARS-	NNP
cord-276978-xl4u7n6r	2	38	and	and	CC
cord-276978-xl4u7n6r	2	39	Other	Other	NNP
cord-276978-xl4u7n6r	2	40	Coronaviruses	Coronaviruses	NNP
cord-276978-xl4u7n6r	3	1	DOI	DOI	NNP
cord-276978-xl4u7n6r	3	2	:	:	:
cord-276978-xl4u7n6r	4	1	10.1007/978	10.1007/978	LS
cord-276978-xl4u7n6r	4	2	-	-	HYPH
cord-276978-xl4u7n6r	4	3	1	1	CD
cord-276978-xl4u7n6r	4	4	-	-	HYPH
cord-276978-xl4u7n6r	4	5	59745	59745	CD
cord-276978-xl4u7n6r	4	6	-	-	HYPH
cord-276978-xl4u7n6r	4	7	181	181	CD
cord-276978-xl4u7n6r	4	8	-	-	SYM
cord-276978-xl4u7n6r	4	9	9_3	9_3	CD
cord-276978-xl4u7n6r	4	10	sha	sha	NN
cord-276978-xl4u7n6r	4	11	:	:	:
cord-276978-xl4u7n6r	4	12	389d0e7bcfe7b090fc97ac3f5ed8406558401945	389d0e7bcfe7b090fc97ac3f5ed8406558401945	NNP
cord-276978-xl4u7n6r	4	13	doc_id	doc_id	CD
cord-276978-xl4u7n6r	4	14	:	:	:
cord-276978-xl4u7n6r	4	15	276978	276978	CD
cord-276978-xl4u7n6r	4	16	cord_uid	cord_uid	NNS
cord-276978-xl4u7n6r	4	17	:	:	:
cord-276978-xl4u7n6r	5	1	xl4u7n6r	xl4u7n6r	NNP
cord-276978-xl4u7n6r	6	1	A	a	DT
cord-276978-xl4u7n6r	6	2	remarkably	remarkably	RB
cord-276978-xl4u7n6r	6	3	conserved	conserve	VBN
cord-276978-xl4u7n6r	6	4	43-nucleotide	43-nucleotide	CD
cord-276978-xl4u7n6r	6	5	-	-	HYPH
cord-276978-xl4u7n6r	6	6	long	long	JJ
cord-276978-xl4u7n6r	6	7	motif	motif	NN
cord-276978-xl4u7n6r	6	8	present	present	JJ
cord-276978-xl4u7n6r	6	9	at	at	IN
cord-276978-xl4u7n6r	6	10	the	the	DT
cord-276978-xl4u7n6r	6	11	3′-end	3′-end	CD
cord-276978-xl4u7n6r	6	12	of	of	IN
cord-276978-xl4u7n6r	6	13	the	the	DT
cord-276978-xl4u7n6r	6	14	genomes	genome	NNS
cord-276978-xl4u7n6r	6	15	of	of	IN
cord-276978-xl4u7n6r	6	16	several	several	JJ
cord-276978-xl4u7n6r	6	17	members	member	NNS
cord-276978-xl4u7n6r	6	18	of	of	IN
cord-276978-xl4u7n6r	6	19	the	the	DT
cord-276978-xl4u7n6r	6	20	polyadenylated	polyadenylated	JJ
cord-276978-xl4u7n6r	6	21	RNA	RNA	NNP
cord-276978-xl4u7n6r	6	22	virus	virus	NN
cord-276978-xl4u7n6r	6	23	families	family	NNS
cord-276978-xl4u7n6r	6	24	Astroviridae	Astroviridae	NNP
cord-276978-xl4u7n6r	6	25	,	,	,
cord-276978-xl4u7n6r	6	26	Coronaviridae	Coronaviridae	NNP
cord-276978-xl4u7n6r	6	27	,	,	,
cord-276978-xl4u7n6r	6	28	and	and	CC
cord-276978-xl4u7n6r	6	29	Picornaviridae	Picornaviridae	NNP
cord-276978-xl4u7n6r	6	30	can	can	MD
cord-276978-xl4u7n6r	6	31	be	be	VB
cord-276978-xl4u7n6r	6	32	used	use	VBN
cord-276978-xl4u7n6r	6	33	for	for	IN
cord-276978-xl4u7n6r	6	34	the	the	DT
cord-276978-xl4u7n6r	6	35	detection	detection	NN
cord-276978-xl4u7n6r	6	36	and	and	CC
cord-276978-xl4u7n6r	6	37	sequence	sequence	NN
cord-276978-xl4u7n6r	6	38	characterization	characterization	NN
cord-276978-xl4u7n6r	6	39	of	of	IN
cord-276978-xl4u7n6r	6	40	the	the	DT
cord-276978-xl4u7n6r	6	41	viruses	virus	NNS
cord-276978-xl4u7n6r	6	42	harboring	harbor	VBG
cord-276978-xl4u7n6r	6	43	it	-PRON-	PRP
cord-276978-xl4u7n6r	6	44	.	.	.
cord-276978-xl4u7n6r	7	1	The	the	DT
cord-276978-xl4u7n6r	7	2	procedure	procedure	NN
cord-276978-xl4u7n6r	7	3	makes	make	VBZ
cord-276978-xl4u7n6r	7	4	use	use	NN
cord-276978-xl4u7n6r	7	5	of	of	IN
cord-276978-xl4u7n6r	7	6	a	a	DT
cord-276978-xl4u7n6r	7	7	primer	primer	NN
cord-276978-xl4u7n6r	7	8	located	locate	VBN
cord-276978-xl4u7n6r	7	9	in	in	IN
cord-276978-xl4u7n6r	7	10	the	the	DT
cord-276978-xl4u7n6r	7	11	most	most	RBS
cord-276978-xl4u7n6r	7	12	conserved	conserved	JJ
cord-276978-xl4u7n6r	7	13	core	core	NN
cord-276978-xl4u7n6r	7	14	of	of	IN
cord-276978-xl4u7n6r	7	15	s2	s2	NNP
cord-276978-xl4u7n6r	7	16	m	m	NNP
cord-276978-xl4u7n6r	7	17	toward	toward	IN
cord-276978-xl4u7n6r	7	18	a	a	DT
cord-276978-xl4u7n6r	7	19	generic	generic	JJ
cord-276978-xl4u7n6r	7	20	anchored	anchor	VBN
cord-276978-xl4u7n6r	7	21	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	7	22	)	)	-RRB-
cord-276978-xl4u7n6r	7	23	primer	primer	NN
cord-276978-xl4u7n6r	7	24	in	in	IN
cord-276978-xl4u7n6r	7	25	a	a	DT
cord-276978-xl4u7n6r	7	26	semispecific	semispecific	NNP
cord-276978-xl4u7n6r	7	27	PCR	PCR	NNP
cord-276978-xl4u7n6r	7	28	.	.	.
cord-276978-xl4u7n6r	8	1	This	this	DT
cord-276978-xl4u7n6r	8	2	strategy	strategy	NN
cord-276978-xl4u7n6r	8	3	allows	allow	VBZ
cord-276978-xl4u7n6r	8	4	the	the	DT
cord-276978-xl4u7n6r	8	5	sequencing	sequencing	NN
cord-276978-xl4u7n6r	8	6	of	of	IN
cord-276978-xl4u7n6r	8	7	some	some	DT
cord-276978-xl4u7n6r	8	8	50–100	50–100	CD
cord-276978-xl4u7n6r	8	9	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	8	10	from	from	IN
cord-276978-xl4u7n6r	8	11	the	the	DT
cord-276978-xl4u7n6r	8	12	3′-end	3′-end	CD
cord-276978-xl4u7n6r	8	13	of	of	IN
cord-276978-xl4u7n6r	8	14	the	the	DT
cord-276978-xl4u7n6r	8	15	virus	virus	NN
cord-276978-xl4u7n6r	8	16	genome	genome	NN
cord-276978-xl4u7n6r	8	17	,	,	,
cord-276978-xl4u7n6r	8	18	representing	represent	VBG
cord-276978-xl4u7n6r	8	19	sufficient	sufficient	JJ
cord-276978-xl4u7n6r	8	20	sequence	sequence	NN
cord-276978-xl4u7n6r	8	21	information	information	NN
cord-276978-xl4u7n6r	8	22	for	for	IN
cord-276978-xl4u7n6r	8	23	initiation	initiation	NN
cord-276978-xl4u7n6r	8	24	of	of	IN
cord-276978-xl4u7n6r	8	25	further	further	JJ
cord-276978-xl4u7n6r	8	26	genomic	genomic	JJ
cord-276978-xl4u7n6r	8	27	characterization	characterization	NN
cord-276978-xl4u7n6r	8	28	in	in	IN
cord-276978-xl4u7n6r	8	29	a	a	DT
cord-276978-xl4u7n6r	8	30	rapid	rapid	JJ
cord-276978-xl4u7n6r	8	31	amplification	amplification	NN
cord-276978-xl4u7n6r	8	32	of	of	IN
cord-276978-xl4u7n6r	8	33	cDNA	cdna	NN
cord-276978-xl4u7n6r	8	34	ends	end	NNS
cord-276978-xl4u7n6r	8	35	(	(	-LRB-
cord-276978-xl4u7n6r	8	36	5′-RACE	5′-race	CD
cord-276978-xl4u7n6r	8	37	)	)	-RRB-
cord-276978-xl4u7n6r	8	38	and	and	CC
cord-276978-xl4u7n6r	8	39	primer	primer	VB
cord-276978-xl4u7n6r	8	40	walking	walking	NN
cord-276978-xl4u7n6r	8	41	strategy	strategy	NN
cord-276978-xl4u7n6r	8	42	.	.	.
cord-276978-xl4u7n6r	9	1	S2	S2	NNP
cord-276978-xl4u7n6r	9	2	m	m	NNP
cord-276978-xl4u7n6r	9	3	is	be	VBZ
cord-276978-xl4u7n6r	9	4	a	a	DT
cord-276978-xl4u7n6r	9	5	conserved	conserved	JJ
cord-276978-xl4u7n6r	9	6	motif	motif	NN
cord-276978-xl4u7n6r	9	7	present	present	JJ
cord-276978-xl4u7n6r	9	8	in	in	IN
cord-276978-xl4u7n6r	9	9	the	the	DT
cord-276978-xl4u7n6r	9	10	genomes	genome	NNS
cord-276978-xl4u7n6r	9	11	of	of	IN
cord-276978-xl4u7n6r	9	12	several	several	JJ
cord-276978-xl4u7n6r	9	13	members	member	NNS
cord-276978-xl4u7n6r	9	14	of	of	IN
cord-276978-xl4u7n6r	9	15	the	the	DT
cord-276978-xl4u7n6r	9	16	RNA	RNA	NNP
cord-276978-xl4u7n6r	9	17	virus	virus	NN
cord-276978-xl4u7n6r	9	18	families	family	NNS
cord-276978-xl4u7n6r	9	19	Astroviridae	Astroviridae	NNP
cord-276978-xl4u7n6r	9	20	,	,	,
cord-276978-xl4u7n6r	9	21	Coronaviridae	Coronaviridae	NNP
cord-276978-xl4u7n6r	9	22	,	,	,
cord-276978-xl4u7n6r	9	23	and	and	CC
cord-276978-xl4u7n6r	9	24	Picornaviridae	Picornaviridae	NNP
cord-276978-xl4u7n6r	9	25	(	(	-LRB-
cord-276978-xl4u7n6r	9	26	1	1	CD
cord-276978-xl4u7n6r	9	27	)	)	-RRB-
cord-276978-xl4u7n6r	9	28	.	.	.
cord-276978-xl4u7n6r	10	1	These	these	DT
cord-276978-xl4u7n6r	10	2	viruses	virus	NNS
cord-276978-xl4u7n6r	10	3	have	have	VBP
cord-276978-xl4u7n6r	10	4	single	single	RB
cord-276978-xl4u7n6r	10	5	-	-	HYPH
cord-276978-xl4u7n6r	10	6	stranded	strand	VBN
cord-276978-xl4u7n6r	10	7	positive	positive	JJ
cord-276978-xl4u7n6r	10	8	-	-	HYPH
cord-276978-xl4u7n6r	10	9	sense	sense	NN
cord-276978-xl4u7n6r	10	10	RNA	RNA	NNP
cord-276978-xl4u7n6r	10	11	genomes	genome	NNS
cord-276978-xl4u7n6r	10	12	,	,	,
cord-276978-xl4u7n6r	10	13	6.5	6.5	CD
cord-276978-xl4u7n6r	10	14	to	to	TO
cord-276978-xl4u7n6r	10	15	32	32	CD
cord-276978-xl4u7n6r	10	16	kb	kb	NNP
cord-276978-xl4u7n6r	10	17	long	long	RB
cord-276978-xl4u7n6r	10	18	,	,	,
cord-276978-xl4u7n6r	10	19	with	with	IN
cord-276978-xl4u7n6r	10	20	a	a	DT
cord-276978-xl4u7n6r	10	21	poly(A	poly(a	JJ
cord-276978-xl4u7n6r	10	22	)	)	-RRB-
cord-276978-xl4u7n6r	10	23	tail	tail	NN
cord-276978-xl4u7n6r	10	24	at	at	IN
cord-276978-xl4u7n6r	10	25	their	-PRON-	PRP$
cord-276978-xl4u7n6r	10	26	3	3	CD
cord-276978-xl4u7n6r	10	27	-ends	-end	NNS
cord-276978-xl4u7n6r	10	28	.	.	.
cord-276978-xl4u7n6r	11	1	S2	S2	NNP
cord-276978-xl4u7n6r	11	2	m	m	NNP
cord-276978-xl4u7n6r	11	3	is	be	VBZ
cord-276978-xl4u7n6r	11	4	43	43	CD
cord-276978-xl4u7n6r	11	5	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	11	6	long	long	JJ
cord-276978-xl4u7n6r	11	7	and	and	CC
cord-276978-xl4u7n6r	11	8	forms	form	VBZ
cord-276978-xl4u7n6r	11	9	a	a	DT
cord-276978-xl4u7n6r	11	10	highly	highly	RB
cord-276978-xl4u7n6r	11	11	conserved	conserve	VBN
cord-276978-xl4u7n6r	11	12	RNA	RNA	NNP
cord-276978-xl4u7n6r	11	13	structure	structure	NN
cord-276978-xl4u7n6r	11	14	(	(	-LRB-
cord-276978-xl4u7n6r	11	15	2	2	CD
cord-276978-xl4u7n6r	11	16	)	)	-RRB-
cord-276978-xl4u7n6r	11	17	.	.	.
cord-276978-xl4u7n6r	12	1	In	in	IN
cord-276978-xl4u7n6r	12	2	these	these	DT
cord-276978-xl4u7n6r	12	3	very	very	RB
cord-276978-xl4u7n6r	12	4	different	different	JJ
cord-276978-xl4u7n6r	12	5	and	and	CC
cord-276978-xl4u7n6r	12	6	rapidly	rapidly	RB
cord-276978-xl4u7n6r	12	7	evolving	evolve	VBG
cord-276978-xl4u7n6r	12	8	viruses	virus	NNS
cord-276978-xl4u7n6r	12	9	,	,	,
cord-276978-xl4u7n6r	12	10	the	the	DT
cord-276978-xl4u7n6r	12	11	remarkably	remarkably	RB
cord-276978-xl4u7n6r	12	12	conserved	conserved	JJ
cord-276978-xl4u7n6r	12	13	nature	nature	NN
cord-276978-xl4u7n6r	12	14	of	of	IN
cord-276978-xl4u7n6r	12	15	s2	s2	NNP
cord-276978-xl4u7n6r	12	16	m	m	NNP
cord-276978-xl4u7n6r	12	17	,	,	,
cord-276978-xl4u7n6r	12	18	in	in	IN
cord-276978-xl4u7n6r	12	19	both	both	DT
cord-276978-xl4u7n6r	12	20	RNA	RNA	NNP
cord-276978-xl4u7n6r	12	21	sequence	sequence	NN
cord-276978-xl4u7n6r	12	22	and	and	CC
cord-276978-xl4u7n6r	12	23	folding	folding	NN
cord-276978-xl4u7n6r	12	24	,	,	,
cord-276978-xl4u7n6r	12	25	suggests	suggest	VBZ
cord-276978-xl4u7n6r	12	26	an	an	DT
cord-276978-xl4u7n6r	12	27	essential	essential	JJ
cord-276978-xl4u7n6r	12	28	,	,	,
cord-276978-xl4u7n6r	12	29	but	but	CC
cord-276978-xl4u7n6r	12	30	as	as	RB
cord-276978-xl4u7n6r	12	31	yet	yet	RB
cord-276978-xl4u7n6r	12	32	unknown	unknown	JJ
cord-276978-xl4u7n6r	12	33	function	function	NN
cord-276978-xl4u7n6r	12	34	.	.	.
cord-276978-xl4u7n6r	13	1	This	this	DT
cord-276978-xl4u7n6r	13	2	motif	motif	NN
cord-276978-xl4u7n6r	13	3	is	be	VBZ
cord-276978-xl4u7n6r	13	4	located	locate	VBN
cord-276978-xl4u7n6r	13	5	C	C	NNP
cord-276978-xl4u7n6r	13	6	Humana	Humana	NNP
cord-276978-xl4u7n6r	13	7	Press	Press	NNP
cord-276978-xl4u7n6r	13	8	,	,	,
cord-276978-xl4u7n6r	13	9	New	New	NNP
cord-276978-xl4u7n6r	13	10	York	York	NNP
cord-276978-xl4u7n6r	13	11	,	,	,
cord-276978-xl4u7n6r	13	12	NY	NY	NNP
cord-276978-xl4u7n6r	13	13	3	3	CD
cord-276978-xl4u7n6r	13	14	,	,	,
cord-276978-xl4u7n6r	13	15	50	50	CD
cord-276978-xl4u7n6r	13	16	-	-	SYM
cord-276978-xl4u7n6r	13	17	150	150	CD
cord-276978-xl4u7n6r	13	18	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	13	19	upstream	upstream	RB
cord-276978-xl4u7n6r	13	20	of	of	IN
cord-276978-xl4u7n6r	13	21	the	the	DT
cord-276978-xl4u7n6r	13	22	3	3	CD
cord-276978-xl4u7n6r	13	23	'	'	NN
cord-276978-xl4u7n6r	13	24	poly(A	poly(a	JJ
cord-276978-xl4u7n6r	13	25	)	)	-RRB-
cord-276978-xl4u7n6r	13	26	tail	tail	NN
cord-276978-xl4u7n6r	13	27	of	of	IN
cord-276978-xl4u7n6r	13	28	these	these	DT
cord-276978-xl4u7n6r	13	29	virus	virus	NN
cord-276978-xl4u7n6r	13	30	genomes	genome	NNS
cord-276978-xl4u7n6r	13	31	.	.	.
cord-276978-xl4u7n6r	14	1	Phylogenetic	phylogenetic	JJ
cord-276978-xl4u7n6r	14	2	considerations	consideration	NNS
cord-276978-xl4u7n6r	14	3	and	and	CC
cord-276978-xl4u7n6r	14	4	the	the	DT
cord-276978-xl4u7n6r	14	5	genomic	genomic	JJ
cord-276978-xl4u7n6r	14	6	position	position	NN
cord-276978-xl4u7n6r	14	7	of	of	IN
cord-276978-xl4u7n6r	14	8	s2	s2	NNP
cord-276978-xl4u7n6r	14	9	m	m	NNP
cord-276978-xl4u7n6r	14	10	suggest	suggest	VBP
cord-276978-xl4u7n6r	14	11	that	that	IN
cord-276978-xl4u7n6r	14	12	ancestors	ancestor	NNS
cord-276978-xl4u7n6r	14	13	of	of	IN
cord-276978-xl4u7n6r	14	14	some	some	DT
cord-276978-xl4u7n6r	14	15	of	of	IN
cord-276978-xl4u7n6r	14	16	these	these	DT
cord-276978-xl4u7n6r	14	17	viruses	virus	NNS
cord-276978-xl4u7n6r	14	18	acquired	acquire	VBD
cord-276978-xl4u7n6r	14	19	s2	s2	NNP
cord-276978-xl4u7n6r	14	20	m	m	NNP
cord-276978-xl4u7n6r	14	21	by	by	IN
cord-276978-xl4u7n6r	14	22	horizontal	horizontal	JJ
cord-276978-xl4u7n6r	14	23	gene	gene	NN
cord-276978-xl4u7n6r	14	24	transfer	transfer	NN
cord-276978-xl4u7n6r	14	25	.	.	.
cord-276978-xl4u7n6r	15	1	All	all	DT
cord-276978-xl4u7n6r	15	2	of	of	IN
cord-276978-xl4u7n6r	15	3	the	the	DT
cord-276978-xl4u7n6r	15	4	known	known	JJ
cord-276978-xl4u7n6r	15	5	group	group	NN
cord-276978-xl4u7n6r	15	6	3	3	CD
cord-276978-xl4u7n6r	15	7	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	15	8	,	,	,
cord-276978-xl4u7n6r	15	9	including	include	VBG
cord-276978-xl4u7n6r	15	10	infectious	infectious	JJ
cord-276978-xl4u7n6r	15	11	bronchitis	bronchitis	NN
cord-276978-xl4u7n6r	15	12	virus	virus	NN
cord-276978-xl4u7n6r	15	13	(	(	-LRB-
cord-276978-xl4u7n6r	15	14	IBV	IBV	NNP
cord-276978-xl4u7n6r	15	15	)	)	-RRB-
cord-276978-xl4u7n6r	15	16	,	,	,
cord-276978-xl4u7n6r	15	17	turkey	turkey	NN
cord-276978-xl4u7n6r	15	18	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	15	19	,	,	,
cord-276978-xl4u7n6r	15	20	pheasant	pheasant	NN
cord-276978-xl4u7n6r	15	21	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	15	22	,	,	,
cord-276978-xl4u7n6r	15	23	and	and	CC
cord-276978-xl4u7n6r	15	24	the	the	DT
cord-276978-xl4u7n6r	15	25	recently	recently	RB
cord-276978-xl4u7n6r	15	26	characterized	characterize	VBN
cord-276978-xl4u7n6r	15	27	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	15	28	infecting	infect	VBG
cord-276978-xl4u7n6r	15	29	different	different	JJ
cord-276978-xl4u7n6r	15	30	wild	wild	JJ
cord-276978-xl4u7n6r	15	31	bird	bird	NN
cord-276978-xl4u7n6r	15	32	species	specie	NNS
cord-276978-xl4u7n6r	15	33	(	(	-LRB-
cord-276978-xl4u7n6r	15	34	3	3	CD
cord-276978-xl4u7n6r	15	35	)	)	-RRB-
cord-276978-xl4u7n6r	15	36	,	,	,
cord-276978-xl4u7n6r	15	37	as	as	RB
cord-276978-xl4u7n6r	15	38	well	well	RB
cord-276978-xl4u7n6r	15	39	as	as	IN
cord-276978-xl4u7n6r	15	40	the	the	DT
cord-276978-xl4u7n6r	15	41	group	group	NN
cord-276978-xl4u7n6r	15	42	2	2	CD
cord-276978-xl4u7n6r	15	43	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	15	44	associated	associate	VBN
cord-276978-xl4u7n6r	15	45	with	with	IN
cord-276978-xl4u7n6r	15	46	the	the	DT
cord-276978-xl4u7n6r	15	47	severe	severe	JJ
cord-276978-xl4u7n6r	15	48	acute	acute	JJ
cord-276978-xl4u7n6r	15	49	respiratory	respiratory	JJ
cord-276978-xl4u7n6r	15	50	syndrome	syndrome	NN
cord-276978-xl4u7n6r	15	51	(	(	-LRB-
cord-276978-xl4u7n6r	15	52	SARS	SARS	NNP
cord-276978-xl4u7n6r	15	53	)	)	-RRB-
cord-276978-xl4u7n6r	15	54	(	(	-LRB-
cord-276978-xl4u7n6r	15	55	4	4	CD
cord-276978-xl4u7n6r	15	56	,	,	,
cord-276978-xl4u7n6r	15	57	5	5	CD
cord-276978-xl4u7n6r	15	58	)	)	-RRB-
cord-276978-xl4u7n6r	15	59	have	have	VBP
cord-276978-xl4u7n6r	15	60	been	be	VBN
cord-276978-xl4u7n6r	15	61	found	find	VBN
cord-276978-xl4u7n6r	15	62	to	to	TO
cord-276978-xl4u7n6r	15	63	have	have	VB
cord-276978-xl4u7n6r	15	64	s2	s2	NNP
cord-276978-xl4u7n6r	15	65	m	m	NNP
cord-276978-xl4u7n6r	15	66	.	.	.
cord-276978-xl4u7n6r	16	1	As	as	IN
cord-276978-xl4u7n6r	16	2	the	the	DT
cord-276978-xl4u7n6r	16	3	core	core	NN
cord-276978-xl4u7n6r	16	4	of	of	IN
cord-276978-xl4u7n6r	16	5	s2	s2	NNP
cord-276978-xl4u7n6r	16	6	m	m	NNP
cord-276978-xl4u7n6r	16	7	is	be	VBZ
cord-276978-xl4u7n6r	16	8	highly	highly	RB
cord-276978-xl4u7n6r	16	9	conserved	conserve	VBN
cord-276978-xl4u7n6r	16	10	in	in	IN
cord-276978-xl4u7n6r	16	11	nucleotide	nucleotide	JJ
cord-276978-xl4u7n6r	16	12	sequence	sequence	NN
cord-276978-xl4u7n6r	16	13	,	,	,
cord-276978-xl4u7n6r	16	14	it	-PRON-	PRP
cord-276978-xl4u7n6r	16	15	is	be	VBZ
cord-276978-xl4u7n6r	16	16	an	an	DT
cord-276978-xl4u7n6r	16	17	ideal	ideal	JJ
cord-276978-xl4u7n6r	16	18	target	target	NN
cord-276978-xl4u7n6r	16	19	for	for	IN
cord-276978-xl4u7n6r	16	20	identification	identification	NN
cord-276978-xl4u7n6r	16	21	of	of	IN
cord-276978-xl4u7n6r	16	22	the	the	DT
cord-276978-xl4u7n6r	16	23	viruses	virus	NNS
cord-276978-xl4u7n6r	16	24	that	that	WDT
cord-276978-xl4u7n6r	16	25	contain	contain	VBP
cord-276978-xl4u7n6r	16	26	it	-PRON-	PRP
cord-276978-xl4u7n6r	16	27	,	,	,
cord-276978-xl4u7n6r	16	28	and	and	CC
cord-276978-xl4u7n6r	16	29	,	,	,
cord-276978-xl4u7n6r	16	30	together	together	RB
cord-276978-xl4u7n6r	16	31	with	with	IN
cord-276978-xl4u7n6r	16	32	a	a	DT
cord-276978-xl4u7n6r	16	33	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	16	34	replicase	replicase	NN
cord-276978-xl4u7n6r	16	35	sequence	sequence	NN
cord-276978-xl4u7n6r	16	36	,	,	,
cord-276978-xl4u7n6r	16	37	it	-PRON-	PRP
cord-276978-xl4u7n6r	16	38	was	be	VBD
cord-276978-xl4u7n6r	16	39	one	one	CD
cord-276978-xl4u7n6r	16	40	of	of	IN
cord-276978-xl4u7n6r	16	41	the	the	DT
cord-276978-xl4u7n6r	16	42	probes	probe	NNS
cord-276978-xl4u7n6r	16	43	that	that	WDT
cord-276978-xl4u7n6r	16	44	first	first	RB
cord-276978-xl4u7n6r	16	45	identified	identify	VBD
cord-276978-xl4u7n6r	16	46	the	the	DT
cord-276978-xl4u7n6r	16	47	etiology	etiology	NN
cord-276978-xl4u7n6r	16	48	of	of	IN
cord-276978-xl4u7n6r	16	49	SARS	SARS	NNP
cord-276978-xl4u7n6r	16	50	as	as	IN
cord-276978-xl4u7n6r	16	51	a	a	DT
cord-276978-xl4u7n6r	16	52	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	16	53	(	(	-LRB-
cord-276978-xl4u7n6r	16	54	4,6	4,6	CD
cord-276978-xl4u7n6r	16	55	)	)	-RRB-
cord-276978-xl4u7n6r	16	56	.	.	.
cord-276978-xl4u7n6r	17	1	In	in	IN
cord-276978-xl4u7n6r	17	2	this	this	DT
cord-276978-xl4u7n6r	17	3	chapter	chapter	NN
cord-276978-xl4u7n6r	17	4	the	the	DT
cord-276978-xl4u7n6r	17	5	use	use	NN
cord-276978-xl4u7n6r	17	6	of	of	IN
cord-276978-xl4u7n6r	17	7	s2	s2	NNP
cord-276978-xl4u7n6r	17	8	m	m	NNP
cord-276978-xl4u7n6r	17	9	as	as	IN
cord-276978-xl4u7n6r	17	10	a	a	DT
cord-276978-xl4u7n6r	17	11	handle	handle	NN
cord-276978-xl4u7n6r	17	12	in	in	IN
cord-276978-xl4u7n6r	17	13	amplification	amplification	NN
cord-276978-xl4u7n6r	17	14	strategies	strategy	NNS
cord-276978-xl4u7n6r	17	15	for	for	IN
cord-276978-xl4u7n6r	17	16	obtaining	obtain	VBG
cord-276978-xl4u7n6r	17	17	sequence	sequence	NN
cord-276978-xl4u7n6r	17	18	information	information	NN
cord-276978-xl4u7n6r	17	19	of	of	IN
cord-276978-xl4u7n6r	17	20	novel	novel	JJ
cord-276978-xl4u7n6r	17	21	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	17	22	genomes	genome	NNS
cord-276978-xl4u7n6r	17	23	is	be	VBZ
cord-276978-xl4u7n6r	17	24	described	describe	VBN
cord-276978-xl4u7n6r	17	25	.	.	.
cord-276978-xl4u7n6r	17	26	 	 	_SP
cord-276978-xl4u7n6r	18	1	Determining	determine	VBG
cord-276978-xl4u7n6r	18	2	the	the	DT
cord-276978-xl4u7n6r	18	3	3	3	CD
cord-276978-xl4u7n6r	18	4	-end	-end	NN
cord-276978-xl4u7n6r	18	5	sequences	sequence	NNS
cord-276978-xl4u7n6r	18	6	of	of	IN
cord-276978-xl4u7n6r	18	7	unknown	unknown	JJ
cord-276978-xl4u7n6r	18	8	RNA	RNA	NNP
cord-276978-xl4u7n6r	18	9	can	can	MD
cord-276978-xl4u7n6r	18	10	be	be	VB
cord-276978-xl4u7n6r	18	11	cumbersome	cumbersome	JJ
cord-276978-xl4u7n6r	18	12	,	,	,
cord-276978-xl4u7n6r	18	13	and	and	CC
cord-276978-xl4u7n6r	18	14	the	the	DT
cord-276978-xl4u7n6r	18	15	present	present	JJ
cord-276978-xl4u7n6r	18	16	method	method	NN
cord-276978-xl4u7n6r	18	17	makes	make	VBZ
cord-276978-xl4u7n6r	18	18	use	use	NN
cord-276978-xl4u7n6r	18	19	of	of	IN
cord-276978-xl4u7n6r	18	20	s2	s2	NNP
cord-276978-xl4u7n6r	18	21	m	m	NNP
cord-276978-xl4u7n6r	18	22	in	in	IN
cord-276978-xl4u7n6r	18	23	some	some	DT
cord-276978-xl4u7n6r	18	24	of	of	IN
cord-276978-xl4u7n6r	18	25	the	the	DT
cord-276978-xl4u7n6r	18	26	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	18	27	genomes	genome	NNS
cord-276978-xl4u7n6r	18	28	as	as	IN
cord-276978-xl4u7n6r	18	29	a	a	DT
cord-276978-xl4u7n6r	18	30	handle	handle	NN
cord-276978-xl4u7n6r	18	31	for	for	IN
cord-276978-xl4u7n6r	18	32	determination	determination	NN
cord-276978-xl4u7n6r	18	33	of	of	IN
cord-276978-xl4u7n6r	18	34	the	the	DT
cord-276978-xl4u7n6r	18	35	sequences	sequence	NNS
cord-276978-xl4u7n6r	18	36	between	between	IN
cord-276978-xl4u7n6r	18	37	s2	s2	NNP
cord-276978-xl4u7n6r	18	38	m	m	NNP
cord-276978-xl4u7n6r	18	39	and	and	CC
cord-276978-xl4u7n6r	18	40	the	the	DT
cord-276978-xl4u7n6r	18	41	3	3	CD
cord-276978-xl4u7n6r	18	42	poly(A	poly(a	VBP
cord-276978-xl4u7n6r	18	43	)	)	-RRB-
cord-276978-xl4u7n6r	18	44	tail	tail	NN
cord-276978-xl4u7n6r	18	45	of	of	IN
cord-276978-xl4u7n6r	18	46	these	these	DT
cord-276978-xl4u7n6r	18	47	genomes	genome	NNS
cord-276978-xl4u7n6r	18	48	.	.	.
cord-276978-xl4u7n6r	19	1	The	the	DT
cord-276978-xl4u7n6r	19	2	method	method	NN
cord-276978-xl4u7n6r	19	3	can	can	MD
cord-276978-xl4u7n6r	19	4	be	be	VB
cord-276978-xl4u7n6r	19	5	used	use	VBN
cord-276978-xl4u7n6r	19	6	on	on	IN
cord-276978-xl4u7n6r	19	7	RNA	RNA	NNP
cord-276978-xl4u7n6r	19	8	extracted	extract	VBN
cord-276978-xl4u7n6r	19	9	from	from	IN
cord-276978-xl4u7n6r	19	10	clinical	clinical	JJ
cord-276978-xl4u7n6r	19	11	samples	sample	NNS
cord-276978-xl4u7n6r	19	12	(	(	-LRB-
cord-276978-xl4u7n6r	19	13	e.g.	e.g.	RB
cord-276978-xl4u7n6r	19	14	,	,	,
cord-276978-xl4u7n6r	19	15	tracheal	tracheal	JJ
cord-276978-xl4u7n6r	19	16	or	or	CC
cord-276978-xl4u7n6r	19	17	cloacal	cloacal	JJ
cord-276978-xl4u7n6r	19	18	swabs	swab	NNS
cord-276978-xl4u7n6r	19	19	)	)	-RRB-
cord-276978-xl4u7n6r	19	20	that	that	WDT
cord-276978-xl4u7n6r	19	21	have	have	VBP
cord-276978-xl4u7n6r	19	22	been	be	VBN
cord-276978-xl4u7n6r	19	23	confirmed	confirm	VBN
cord-276978-xl4u7n6r	19	24	to	to	TO
cord-276978-xl4u7n6r	19	25	be	be	VB
cord-276978-xl4u7n6r	19	26	positive	positive	JJ
cord-276978-xl4u7n6r	19	27	for	for	IN
cord-276978-xl4u7n6r	19	28	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	19	29	,	,	,
cord-276978-xl4u7n6r	19	30	e.g.	e.g.	RB
cord-276978-xl4u7n6r	19	31	,	,	,
cord-276978-xl4u7n6r	19	32	by	by	IN
cord-276978-xl4u7n6r	19	33	RT	RT	NNP
cord-276978-xl4u7n6r	19	34	-	-	HYPH
cord-276978-xl4u7n6r	19	35	PCR	PCR	NNP
cord-276978-xl4u7n6r	19	36	and	and	CC
cord-276978-xl4u7n6r	19	37	sequencing	sequencing	NN
cord-276978-xl4u7n6r	19	38	,	,	,
cord-276978-xl4u7n6r	19	39	using	use	VBG
cord-276978-xl4u7n6r	19	40	pancoronavirus	pancoronavirus	NNP
cord-276978-xl4u7n6r	19	41	primers	primer	NNS
cord-276978-xl4u7n6r	19	42	(	(	-LRB-
cord-276978-xl4u7n6r	19	43	7	7	CD
cord-276978-xl4u7n6r	19	44	)	)	-RRB-
cord-276978-xl4u7n6r	19	45	(	(	-LRB-
cord-276978-xl4u7n6r	19	46	see	see	VB
cord-276978-xl4u7n6r	19	47	Note	Note	NNP
cord-276978-xl4u7n6r	19	48	1	1	CD
cord-276978-xl4u7n6r	19	49	)	)	-RRB-
cord-276978-xl4u7n6r	19	50	.	.	.
cord-276978-xl4u7n6r	20	1	As	as	IN
cord-276978-xl4u7n6r	20	2	s2	s2	NNP
cord-276978-xl4u7n6r	20	3	m	m	NNP
cord-276978-xl4u7n6r	20	4	is	be	VBZ
cord-276978-xl4u7n6r	20	5	located	locate	VBN
cord-276978-xl4u7n6r	20	6	only	only	RB
cord-276978-xl4u7n6r	20	7	about	about	RB
cord-276978-xl4u7n6r	20	8	100	100	CD
cord-276978-xl4u7n6r	20	9	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	20	10	upstream	upstream	RB
cord-276978-xl4u7n6r	20	11	of	of	IN
cord-276978-xl4u7n6r	20	12	the	the	DT
cord-276978-xl4u7n6r	20	13	3	3	CD
cord-276978-xl4u7n6r	20	14	poly(A	poly(a	JJ
cord-276978-xl4u7n6r	20	15	)	)	-RRB-
cord-276978-xl4u7n6r	20	16	tail	tail	NN
cord-276978-xl4u7n6r	20	17	when	when	WRB
cord-276978-xl4u7n6r	20	18	it	-PRON-	PRP
cord-276978-xl4u7n6r	20	19	is	be	VBZ
cord-276978-xl4u7n6r	20	20	present	present	JJ
cord-276978-xl4u7n6r	20	21	in	in	IN
cord-276978-xl4u7n6r	20	22	the	the	DT
cord-276978-xl4u7n6r	20	23	genomes	genome	NNS
cord-276978-xl4u7n6r	20	24	of	of	IN
cord-276978-xl4u7n6r	20	25	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	20	26	,	,	,
cord-276978-xl4u7n6r	20	27	only	only	RB
cord-276978-xl4u7n6r	20	28	limited	limited	JJ
cord-276978-xl4u7n6r	20	29	sequence	sequence	NN
cord-276978-xl4u7n6r	20	30	information	information	NN
cord-276978-xl4u7n6r	20	31	can	can	MD
cord-276978-xl4u7n6r	20	32	be	be	VB
cord-276978-xl4u7n6r	20	33	acertained	acertaine	VBN
cord-276978-xl4u7n6r	20	34	from	from	IN
cord-276978-xl4u7n6r	20	35	the	the	DT
cord-276978-xl4u7n6r	20	36	initial	initial	JJ
cord-276978-xl4u7n6r	20	37	amplification	amplification	NN
cord-276978-xl4u7n6r	20	38	product	product	NN
cord-276978-xl4u7n6r	20	39	obtained	obtain	VBN
cord-276978-xl4u7n6r	20	40	using	use	VBG
cord-276978-xl4u7n6r	20	41	a	a	DT
cord-276978-xl4u7n6r	20	42	primer	primer	NN
cord-276978-xl4u7n6r	20	43	located	locate	VBN
cord-276978-xl4u7n6r	20	44	within	within	IN
cord-276978-xl4u7n6r	20	45	s2	s2	NNP
cord-276978-xl4u7n6r	20	46	m	m	NNP
cord-276978-xl4u7n6r	20	47	toward	toward	IN
cord-276978-xl4u7n6r	20	48	a	a	DT
cord-276978-xl4u7n6r	20	49	generic	generic	JJ
cord-276978-xl4u7n6r	20	50	primer	primer	NN
cord-276978-xl4u7n6r	20	51	for	for	IN
cord-276978-xl4u7n6r	20	52	polyadenylated	polyadenylated	JJ
cord-276978-xl4u7n6r	20	53	RNA	RNA	NNP
cord-276978-xl4u7n6r	20	54	.	.	.
cord-276978-xl4u7n6r	21	1	This	this	DT
cord-276978-xl4u7n6r	21	2	limited	limited	JJ
cord-276978-xl4u7n6r	21	3	sequence	sequence	NN
cord-276978-xl4u7n6r	21	4	information	information	NN
cord-276978-xl4u7n6r	21	5	is	be	VBZ
cord-276978-xl4u7n6r	21	6	,	,	,
cord-276978-xl4u7n6r	21	7	however	however	RB
cord-276978-xl4u7n6r	21	8	,	,	,
cord-276978-xl4u7n6r	21	9	sufficient	sufficient	JJ
cord-276978-xl4u7n6r	21	10	to	to	TO
cord-276978-xl4u7n6r	21	11	design	design	VB
cord-276978-xl4u7n6r	21	12	virus	virus	NN
cord-276978-xl4u7n6r	21	13	-	-	HYPH
cord-276978-xl4u7n6r	21	14	specific	specific	JJ
cord-276978-xl4u7n6r	21	15	primers	primer	NNS
cord-276978-xl4u7n6r	21	16	that	that	WDT
cord-276978-xl4u7n6r	21	17	can	can	MD
cord-276978-xl4u7n6r	21	18	be	be	VB
cord-276978-xl4u7n6r	21	19	used	use	VBN
cord-276978-xl4u7n6r	21	20	in	in	IN
cord-276978-xl4u7n6r	21	21	a	a	DT
cord-276978-xl4u7n6r	21	22	5	5	CD
cord-276978-xl4u7n6r	21	23	-RACE	-race	NN
cord-276978-xl4u7n6r	21	24	strategy	strategy	NN
cord-276978-xl4u7n6r	21	25	that	that	WDT
cord-276978-xl4u7n6r	21	26	,	,	,
cord-276978-xl4u7n6r	21	27	together	together	RB
cord-276978-xl4u7n6r	21	28	with	with	IN
cord-276978-xl4u7n6r	21	29	primer	primer	NN
cord-276978-xl4u7n6r	21	30	walking	walking	NN
cord-276978-xl4u7n6r	21	31	,	,	,
cord-276978-xl4u7n6r	21	32	allow	allow	VB
cord-276978-xl4u7n6r	21	33	sequencing	sequencing	NN
cord-276978-xl4u7n6r	21	34	toward	toward	IN
cord-276978-xl4u7n6r	21	35	the	the	DT
cord-276978-xl4u7n6r	21	36	5	5	CD
cord-276978-xl4u7n6r	21	37	-end	-end	:
cord-276978-xl4u7n6r	21	38	of	of	IN
cord-276978-xl4u7n6r	21	39	the	the	DT
cord-276978-xl4u7n6r	21	40	virus	virus	NN
cord-276978-xl4u7n6r	21	41	genomes	genome	NNS
cord-276978-xl4u7n6r	21	42	(	(	-LRB-
cord-276978-xl4u7n6r	21	43	8)	8)	NNP
cord-276978-xl4u7n6r	21	44	.	.	.
cord-276978-xl4u7n6r	21	45	1	1	CD
cord-276978-xl4u7n6r	21	46	.	.	.
cord-276978-xl4u7n6r	22	1	For	for	IN
cord-276978-xl4u7n6r	22	2	RT	RT	NNP
cord-276978-xl4u7n6r	22	3	,	,	,
cord-276978-xl4u7n6r	22	4	prepare	prepare	VB
cord-276978-xl4u7n6r	22	5	the	the	DT
cord-276978-xl4u7n6r	22	6	following	following	JJ
cord-276978-xl4u7n6r	22	7	mix	mix	NN
cord-276978-xl4u7n6r	22	8	(	(	-LRB-
cord-276978-xl4u7n6r	22	9	per	per	IN
cord-276978-xl4u7n6r	22	10	sample	sample	NN
cord-276978-xl4u7n6r	22	11	)	)	-RRB-
cord-276978-xl4u7n6r	22	12	:	:	:
cord-276978-xl4u7n6r	22	13	4	4	CD
cord-276978-xl4u7n6r	22	14	l	l	NN
cord-276978-xl4u7n6r	22	15	5X	5x	CD
cord-276978-xl4u7n6r	22	16	first	first	JJ
cord-276978-xl4u7n6r	22	17	-	-	HYPH
cord-276978-xl4u7n6r	22	18	strand	strand	NN
cord-276978-xl4u7n6r	22	19	buffer	buffer	NN
cord-276978-xl4u7n6r	22	20	,	,	,
cord-276978-xl4u7n6r	22	21	1	1	CD
cord-276978-xl4u7n6r	22	22	l	l	NN
cord-276978-xl4u7n6r	22	23	100	100	CD
cord-276978-xl4u7n6r	22	24	mM	mM	NNP
cord-276978-xl4u7n6r	22	25	DTT	DTT	NNP
cord-276978-xl4u7n6r	22	26	,	,	,
cord-276978-xl4u7n6r	22	27	1	1	CD
cord-276978-xl4u7n6r	22	28	l	l	NN
cord-276978-xl4u7n6r	22	29	dNTP	dntp	NN
cord-276978-xl4u7n6r	23	1	(	(	-LRB-
cord-276978-xl4u7n6r	23	2	10	10	CD
cord-276978-xl4u7n6r	23	3	mM	mm	CD
cord-276978-xl4u7n6r	23	4	)	)	-RRB-
cord-276978-xl4u7n6r	23	5	,	,	,
cord-276978-xl4u7n6r	23	6	1	1	CD
cord-276978-xl4u7n6r	23	7	l	l	NN
cord-276978-xl4u7n6r	23	8	anchored	anchor	VBD
cord-276978-xl4u7n6r	23	9	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	23	10	)	)	-RRB-
cord-276978-xl4u7n6r	23	11	primer	primer	NN
cord-276978-xl4u7n6r	23	12	(	(	-LRB-
cord-276978-xl4u7n6r	23	13	see	see	VB
cord-276978-xl4u7n6r	23	14	Note	Note	NNP
cord-276978-xl4u7n6r	23	15	2	2	CD
cord-276978-xl4u7n6r	23	16	)	)	-RRB-
cord-276978-xl4u7n6r	23	17	,	,	,
cord-276978-xl4u7n6r	23	18	1	1	CD
cord-276978-xl4u7n6r	23	19	l	l	NN
cord-276978-xl4u7n6r	23	20	RNaseOUT	rnaseout	NN
cord-276978-xl4u7n6r	23	21	(	(	-LRB-
cord-276978-xl4u7n6r	23	22	40	40	CD
cord-276978-xl4u7n6r	23	23	U	U	NNP
cord-276978-xl4u7n6r	23	24	/	/	SYM
cord-276978-xl4u7n6r	23	25	l	l	NN
cord-276978-xl4u7n6r	23	26	)	)	-RRB-
cord-276978-xl4u7n6r	23	27	,	,	,
cord-276978-xl4u7n6r	23	28	and	and	CC
cord-276978-xl4u7n6r	23	29	1	1	CD
cord-276978-xl4u7n6r	23	30	l	l	NN
cord-276978-xl4u7n6r	23	31	Superscript	superscript	NN
cord-276978-xl4u7n6r	23	32	III	III	NNP
cord-276978-xl4u7n6r	23	33	reverse	reverse	NN
cord-276978-xl4u7n6r	23	34	transcriptase	transcriptase	NN
cord-276978-xl4u7n6r	23	35	.	.	.
cord-276978-xl4u7n6r	24	1	The	the	DT
cord-276978-xl4u7n6r	24	2	mix	mix	NN
cord-276978-xl4u7n6r	24	3	should	should	MD
cord-276978-xl4u7n6r	24	4	be	be	VB
cord-276978-xl4u7n6r	24	5	made	make	VBN
cord-276978-xl4u7n6r	24	6	fresh	fresh	JJ
cord-276978-xl4u7n6r	24	7	,	,	,
cord-276978-xl4u7n6r	24	8	and	and	CC
cord-276978-xl4u7n6r	24	9	stored	store	VBN
cord-276978-xl4u7n6r	24	10	at	at	IN
cord-276978-xl4u7n6r	24	11	4	4	CD
cord-276978-xl4u7n6r	24	12	•	•	NN
cord-276978-xl4u7n6r	24	13	C	c	NN
cord-276978-xl4u7n6r	24	14	until	until	IN
cord-276978-xl4u7n6r	24	15	use	use	NN
cord-276978-xl4u7n6r	24	16	.	.	.
cord-276978-xl4u7n6r	25	1	2	2	LS
cord-276978-xl4u7n6r	25	2	.	.	.
cord-276978-xl4u7n6r	26	1	Add	add	VB
cord-276978-xl4u7n6r	26	2	11	11	CD
cord-276978-xl4u7n6r	26	3	l	l	NN
cord-276978-xl4u7n6r	26	4	RNA	RNA	NNP
cord-276978-xl4u7n6r	26	5	extract	extract	NN
cord-276978-xl4u7n6r	26	6	to	to	IN
cord-276978-xl4u7n6r	26	7	the	the	DT
cord-276978-xl4u7n6r	26	8	9-l	9-l	CD
cord-276978-xl4u7n6r	26	9	RT	RT	NNP
cord-276978-xl4u7n6r	26	10	mix	mix	NN
cord-276978-xl4u7n6r	26	11	,	,	,
cord-276978-xl4u7n6r	26	12	with	with	IN
cord-276978-xl4u7n6r	26	13	care	care	NN
cord-276978-xl4u7n6r	26	14	taken	take	VBN
cord-276978-xl4u7n6r	26	15	to	to	TO
cord-276978-xl4u7n6r	26	16	avoid	avoid	VB
cord-276978-xl4u7n6r	26	17	air	air	NN
cord-276978-xl4u7n6r	26	18	bubbles	bubble	NNS
cord-276978-xl4u7n6r	26	19	,	,	,
cord-276978-xl4u7n6r	26	20	as	as	IN
cord-276978-xl4u7n6r	26	21	these	these	DT
cord-276978-xl4u7n6r	26	22	can	can	MD
cord-276978-xl4u7n6r	26	23	oxidize	oxidize	VB
cord-276978-xl4u7n6r	26	24	the	the	DT
cord-276978-xl4u7n6r	26	25	reverse	reverse	JJ
cord-276978-xl4u7n6r	26	26	transcriptase	transcriptase	NN
cord-276978-xl4u7n6r	26	27	.	.	.
cord-276978-xl4u7n6r	27	1	The	the	DT
cord-276978-xl4u7n6r	27	2	RT	RT	NNP
cord-276978-xl4u7n6r	27	3	is	be	VBZ
cord-276978-xl4u7n6r	27	4	performed	perform	VBN
cord-276978-xl4u7n6r	27	5	at	at	IN
cord-276978-xl4u7n6r	27	6	50	50	CD
cord-276978-xl4u7n6r	27	7	•	•	NNS
cord-276978-xl4u7n6r	27	8	C	c	NN
cord-276978-xl4u7n6r	27	9	for	for	IN
cord-276978-xl4u7n6r	27	10	30	30	CD
cord-276978-xl4u7n6r	27	11	min	min	NN
cord-276978-xl4u7n6r	27	12	,	,	,
cord-276978-xl4u7n6r	27	13	followed	follow	VBN
cord-276978-xl4u7n6r	27	14	by	by	IN
cord-276978-xl4u7n6r	27	15	an	an	DT
cord-276978-xl4u7n6r	27	16	enzyme	enzyme	NN
cord-276978-xl4u7n6r	27	17	inactivation	inactivation	NN
cord-276978-xl4u7n6r	27	18	step	step	NN
cord-276978-xl4u7n6r	27	19	at	at	IN
cord-276978-xl4u7n6r	27	20	70	70	CD
cord-276978-xl4u7n6r	28	1	•	•	NNP
cord-276978-xl4u7n6r	28	2	C	C	NNP
cord-276978-xl4u7n6r	28	3	for	for	IN
cord-276978-xl4u7n6r	28	4	15	15	CD
cord-276978-xl4u7n6r	28	5	min	min	NN
cord-276978-xl4u7n6r	28	6	,	,	,
cord-276978-xl4u7n6r	28	7	and	and	CC
cord-276978-xl4u7n6r	28	8	cooling	cool	VBG
cord-276978-xl4u7n6r	28	9	to	to	IN
cord-276978-xl4u7n6r	28	10	4	4	CD
cord-276978-xl4u7n6r	28	11	•	•	NN
cord-276978-xl4u7n6r	28	12	C	c	NN
cord-276978-xl4u7n6r	28	13	thereafter	thereafter	RB
cord-276978-xl4u7n6r	28	14	.	.	.
cord-276978-xl4u7n6r	29	1	3	3	LS
cord-276978-xl4u7n6r	29	2	.	.	.
cord-276978-xl4u7n6r	30	1	PCR	PCR	NNP
cord-276978-xl4u7n6r	30	2	is	be	VBZ
cord-276978-xl4u7n6r	30	3	performed	perform	VBN
cord-276978-xl4u7n6r	30	4	using	use	VBG
cord-276978-xl4u7n6r	30	5	the	the	DT
cord-276978-xl4u7n6r	30	6	s2m	s2m	JJ
cord-276978-xl4u7n6r	30	7	-	-	HYPH
cord-276978-xl4u7n6r	30	8	core	core	JJ
cord-276978-xl4u7n6r	30	9	primer	primer	NN
cord-276978-xl4u7n6r	30	10	toward	toward	IN
cord-276978-xl4u7n6r	30	11	a	a	DT
cord-276978-xl4u7n6r	30	12	generic	generic	JJ
cord-276978-xl4u7n6r	30	13	anchored	anchor	VBN
cord-276978-xl4u7n6r	30	14	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	30	15	)	)	-RRB-
cord-276978-xl4u7n6r	30	16	primer	primer	NN
cord-276978-xl4u7n6r	30	17	 	 	_SP
cord-276978-xl4u7n6r	30	18	1	1	CD
cord-276978-xl4u7n6r	30	19	.	.	.
cord-276978-xl4u7n6r	31	1	15	15	CD
cord-276978-xl4u7n6r	31	2	l	l	NN
cord-276978-xl4u7n6r	31	3	of	of	IN
cord-276978-xl4u7n6r	31	4	the	the	DT
cord-276978-xl4u7n6r	31	5	PCR	PCR	NNP
cord-276978-xl4u7n6r	31	6	products	product	NNS
cord-276978-xl4u7n6r	31	7	are	be	VBP
cord-276978-xl4u7n6r	31	8	visualized	visualize	VBN
cord-276978-xl4u7n6r	31	9	on	on	IN
cord-276978-xl4u7n6r	31	10	a	a	DT
cord-276978-xl4u7n6r	31	11	2	2	CD
cord-276978-xl4u7n6r	31	12	%	%	NN
cord-276978-xl4u7n6r	31	13	agarose	agarose	NN
cord-276978-xl4u7n6r	31	14	gel	gel	NN
cord-276978-xl4u7n6r	31	15	.	.	.
cord-276978-xl4u7n6r	32	1	If	if	IN
cord-276978-xl4u7n6r	32	2	s2	s2	NNP
cord-276978-xl4u7n6r	32	3	m	m	NNP
cord-276978-xl4u7n6r	32	4	is	be	VBZ
cord-276978-xl4u7n6r	32	5	part	part	NN
cord-276978-xl4u7n6r	32	6	of	of	IN
cord-276978-xl4u7n6r	32	7	the	the	DT
cord-276978-xl4u7n6r	32	8	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	32	9	genome	genome	NN
cord-276978-xl4u7n6r	32	10	,	,	,
cord-276978-xl4u7n6r	32	11	one	one	PRP
cord-276978-xl4u7n6r	32	12	should	should	MD
cord-276978-xl4u7n6r	32	13	obtain	obtain	VB
cord-276978-xl4u7n6r	32	14	specific	specific	JJ
cord-276978-xl4u7n6r	32	15	amplification	amplification	NN
cord-276978-xl4u7n6r	32	16	of	of	IN
cord-276978-xl4u7n6r	32	17	the	the	DT
cord-276978-xl4u7n6r	32	18	3	3	CD
cord-276978-xl4u7n6r	32	19	-end	-end	:
cord-276978-xl4u7n6r	32	20	(	(	-LRB-
cord-276978-xl4u7n6r	32	21	see	see	VB
cord-276978-xl4u7n6r	32	22	Note	Note	NNP
cord-276978-xl4u7n6r	32	23	3	3	CD
cord-276978-xl4u7n6r	32	24	)	)	-RRB-
cord-276978-xl4u7n6r	32	25	.	.	.
cord-276978-xl4u7n6r	33	1	The	the	DT
cord-276978-xl4u7n6r	33	2	PCR	PCR	NNP
cord-276978-xl4u7n6r	33	3	products	product	NNS
cord-276978-xl4u7n6r	33	4	are	be	VBP
cord-276978-xl4u7n6r	33	5	purified	purify	VBN
cord-276978-xl4u7n6r	33	6	directly	directly	RB
cord-276978-xl4u7n6r	33	7	prior	prior	RB
cord-276978-xl4u7n6r	33	8	to	to	IN
cord-276978-xl4u7n6r	33	9	sequencing	sequencing	NN
cord-276978-xl4u7n6r	33	10	reaction	reaction	NN
cord-276978-xl4u7n6r	33	11	,	,	,
cord-276978-xl4u7n6r	33	12	using	use	VBG
cord-276978-xl4u7n6r	33	13	the	the	DT
cord-276978-xl4u7n6r	33	14	QIAquick	QIAquick	NNP
cord-276978-xl4u7n6r	33	15	PCR	PCR	NNP
cord-276978-xl4u7n6r	33	16	purification	purification	NN
cord-276978-xl4u7n6r	33	17	kit	kit	NN
cord-276978-xl4u7n6r	33	18	according	accord	VBG
cord-276978-xl4u7n6r	33	19	to	to	IN
cord-276978-xl4u7n6r	33	20	the	the	DT
cord-276978-xl4u7n6r	33	21	manufacturer	manufacturer	NN
cord-276978-xl4u7n6r	33	22	's	's	POS
cord-276978-xl4u7n6r	33	23	instructions	instruction	NNS
cord-276978-xl4u7n6r	33	24	,	,	,
cord-276978-xl4u7n6r	33	25	with	with	IN
cord-276978-xl4u7n6r	33	26	a	a	DT
cord-276978-xl4u7n6r	33	27	final	final	JJ
cord-276978-xl4u7n6r	33	28	elution	elution	NN
cord-276978-xl4u7n6r	33	29	volume	volume	NN
cord-276978-xl4u7n6r	33	30	of	of	IN
cord-276978-xl4u7n6r	33	31	30	30	CD
cord-276978-xl4u7n6r	33	32	l.	l.	NN
cord-276978-xl4u7n6r	33	33	2	2	CD
cord-276978-xl4u7n6r	33	34	.	.	.
cord-276978-xl4u7n6r	34	1	The	the	DT
cord-276978-xl4u7n6r	34	2	cycle	cycle	NN
cord-276978-xl4u7n6r	34	3	sequencing	sequencing	NN
cord-276978-xl4u7n6r	34	4	reaction	reaction	NN
cord-276978-xl4u7n6r	34	5	is	be	VBZ
cord-276978-xl4u7n6r	34	6	performed	perform	VBN
cord-276978-xl4u7n6r	34	7	using	use	VBG
cord-276978-xl4u7n6r	34	8	the	the	DT
cord-276978-xl4u7n6r	34	9	BigDye	BigDye	NNP
cord-276978-xl4u7n6r	34	10	Terminator	Terminator	NNP
cord-276978-xl4u7n6r	34	11	version	version	NN
cord-276978-xl4u7n6r	34	12	1.1	1.1	CD
cord-276978-xl4u7n6r	34	13	,	,	,
cord-276978-xl4u7n6r	34	14	especially	especially	RB
cord-276978-xl4u7n6r	34	15	suited	suit	VBN
cord-276978-xl4u7n6r	34	16	for	for	IN
cord-276978-xl4u7n6r	34	17	short	short	JJ
cord-276978-xl4u7n6r	34	18	PCR	PCR	NNP
cord-276978-xl4u7n6r	34	19	products	product	NNS
cord-276978-xl4u7n6r	34	20	.	.	.
cord-276978-xl4u7n6r	35	1	Add	add	VB
cord-276978-xl4u7n6r	35	2	4	4	CD
cord-276978-xl4u7n6r	35	3	l	l	NN
cord-276978-xl4u7n6r	35	4	BigDye	BigDye	NNP
cord-276978-xl4u7n6r	35	5	terminator	terminator	NN
cord-276978-xl4u7n6r	35	6	mix	mix	NN
cord-276978-xl4u7n6r	35	7	,	,	,
cord-276978-xl4u7n6r	35	8	1.3	1.3	CD
cord-276978-xl4u7n6r	35	9	l	l	NN
cord-276978-xl4u7n6r	35	10	primer	primer	NN
cord-276978-xl4u7n6r	35	11	s2m	s2m	JJ
cord-276978-xl4u7n6r	35	12	-	-	HYPH
cord-276978-xl4u7n6r	35	13	coreseq	coreseq	NNP
cord-276978-xl4u7n6r	35	14	(	(	-LRB-
cord-276978-xl4u7n6r	35	15	2.5	2.5	CD
cord-276978-xl4u7n6r	35	16	M	M	NNP
cord-276978-xl4u7n6r	35	17	)	)	-RRB-
cord-276978-xl4u7n6r	35	18	(	(	-LRB-
cord-276978-xl4u7n6r	35	19	see	see	VB
cord-276978-xl4u7n6r	35	20	Note	Note	NNP
cord-276978-xl4u7n6r	35	21	4	4	CD
cord-276978-xl4u7n6r	35	22	)	)	-RRB-
cord-276978-xl4u7n6r	35	23	to	to	IN
cord-276978-xl4u7n6r	35	24	4.7	4.7	CD
cord-276978-xl4u7n6r	35	25	l	l	NN
cord-276978-xl4u7n6r	35	26	purified	purify	VBN
cord-276978-xl4u7n6r	35	27	PCR	PCR	NNP
cord-276978-xl4u7n6r	35	28	product	product	NN
cord-276978-xl4u7n6r	35	29	,	,	,
cord-276978-xl4u7n6r	35	30	and	and	CC
cord-276978-xl4u7n6r	35	31	perform	perform	VB
cord-276978-xl4u7n6r	35	32	the	the	DT
cord-276978-xl4u7n6r	35	33	cycle	cycle	NN
cord-276978-xl4u7n6r	35	34	sequencing	sequencing	NN
cord-276978-xl4u7n6r	35	35	reaction	reaction	NN
cord-276978-xl4u7n6r	35	36	as	as	IN
cord-276978-xl4u7n6r	35	37	follows	follow	VBZ
cord-276978-xl4u7n6r	35	38	:	:	:
cord-276978-xl4u7n6r	35	39	96	96	CD
cord-276978-xl4u7n6r	36	1	•	•	NNP
cord-276978-xl4u7n6r	37	1	C	C	NNP
cord-276978-xl4u7n6r	37	2	for	for	IN
cord-276978-xl4u7n6r	37	3	1	1	CD
cord-276978-xl4u7n6r	37	4	min	min	NN
cord-276978-xl4u7n6r	37	5	,	,	,
cord-276978-xl4u7n6r	37	6	followed	follow	VBN
cord-276978-xl4u7n6r	37	7	by	by	IN
cord-276978-xl4u7n6r	37	8	25	25	CD
cord-276978-xl4u7n6r	37	9	cycles	cycle	NNS
cord-276978-xl4u7n6r	37	10	(	(	-LRB-
cord-276978-xl4u7n6r	37	11	96	96	CD
cord-276978-xl4u7n6r	37	12	•	•	NN
cord-276978-xl4u7n6r	37	13	C	C	NNP
cord-276978-xl4u7n6r	37	14	,	,	,
cord-276978-xl4u7n6r	37	15	10	10	CD
cord-276978-xl4u7n6r	37	16	sec	sec	NNS
cord-276978-xl4u7n6r	37	17	;	;	:
cord-276978-xl4u7n6r	37	18	55	55	CD
cord-276978-xl4u7n6r	37	19	•	•	NNS
cord-276978-xl4u7n6r	37	20	C	c	NN
cord-276978-xl4u7n6r	37	21	,	,	,
cord-276978-xl4u7n6r	37	22	5	5	CD
cord-276978-xl4u7n6r	37	23	sec	sec	NNP
cord-276978-xl4u7n6r	37	24	;	;	:
cord-276978-xl4u7n6r	37	25	60	60	CD
cord-276978-xl4u7n6r	37	26	•	•	NNS
cord-276978-xl4u7n6r	37	27	C	C	NNP
cord-276978-xl4u7n6r	37	28	,	,	,
cord-276978-xl4u7n6r	37	29	4	4	CD
cord-276978-xl4u7n6r	37	30	min	min	NNP
cord-276978-xl4u7n6r	37	31	)	)	-RRB-
cord-276978-xl4u7n6r	37	32	,	,	,
cord-276978-xl4u7n6r	37	33	and	and	CC
cord-276978-xl4u7n6r	37	34	cooling	cool	VBG
cord-276978-xl4u7n6r	37	35	to	to	IN
cord-276978-xl4u7n6r	37	36	4	4	CD
cord-276978-xl4u7n6r	37	37	•	•	NN
cord-276978-xl4u7n6r	37	38	C	c	NN
cord-276978-xl4u7n6r	37	39	thereafter	thereafter	RB
cord-276978-xl4u7n6r	37	40	.	.	.
cord-276978-xl4u7n6r	38	1	3	3	LS
cord-276978-xl4u7n6r	38	2	.	.	.
cord-276978-xl4u7n6r	39	1	The	the	DT
cord-276978-xl4u7n6r	39	2	cycle	cycle	NN
cord-276978-xl4u7n6r	39	3	sequencing	sequencing	NN
cord-276978-xl4u7n6r	39	4	products	product	NNS
cord-276978-xl4u7n6r	39	5	are	be	VBP
cord-276978-xl4u7n6r	39	6	purified	purify	VBN
cord-276978-xl4u7n6r	39	7	from	from	IN
cord-276978-xl4u7n6r	39	8	unincorporated	unincorporated	JJ
cord-276978-xl4u7n6r	39	9	labeled	label	VBN
cord-276978-xl4u7n6r	39	10	dideoxynucleotides	dideoxynucleotide	NNS
cord-276978-xl4u7n6r	39	11	and	and	CC
cord-276978-xl4u7n6r	39	12	separated	separate	VBN
cord-276978-xl4u7n6r	39	13	by	by	IN
cord-276978-xl4u7n6r	39	14	capillary	capillary	JJ
cord-276978-xl4u7n6r	39	15	electrophoresis	electrophoresis	NN
cord-276978-xl4u7n6r	39	16	on	on	IN
cord-276978-xl4u7n6r	39	17	a	a	DT
cord-276978-xl4u7n6r	39	18	3130xl	3130xl	JJ
cord-276978-xl4u7n6r	39	19	Genetic	Genetic	NNP
cord-276978-xl4u7n6r	39	20	Analyser	analyser	NN
cord-276978-xl4u7n6r	39	21	for	for	IN
cord-276978-xl4u7n6r	39	22	sequence	sequence	NN
cord-276978-xl4u7n6r	39	23	determination	determination	NN
cord-276978-xl4u7n6r	39	24	,	,	,
cord-276978-xl4u7n6r	39	25	according	accord	VBG
cord-276978-xl4u7n6r	39	26	to	to	IN
cord-276978-xl4u7n6r	39	27	Applied	Applied	NNP
cord-276978-xl4u7n6r	39	28	Biosystems	Biosystems	NNP
cord-276978-xl4u7n6r	39	29	'	'	POS
cord-276978-xl4u7n6r	39	30	instructions	instruction	NNS
cord-276978-xl4u7n6r	39	31	.	.	.
cord-276978-xl4u7n6r	40	1	4	4	LS
cord-276978-xl4u7n6r	40	2	.	.	.
cord-276978-xl4u7n6r	41	1	The	the	DT
cord-276978-xl4u7n6r	41	2	sequence	sequence	NN
cord-276978-xl4u7n6r	41	3	obtained	obtain	VBD
cord-276978-xl4u7n6r	41	4	gives	give	VBZ
cord-276978-xl4u7n6r	41	5	information	information	NN
cord-276978-xl4u7n6r	41	6	on	on	IN
cord-276978-xl4u7n6r	41	7	the	the	DT
cord-276978-xl4u7n6r	41	8	3	3	CD
cord-276978-xl4u7n6r	41	9	-end	-end	:
cord-276978-xl4u7n6r	41	10	of	of	IN
cord-276978-xl4u7n6r	41	11	the	the	DT
cord-276978-xl4u7n6r	41	12	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	41	13	genome	genome	NN
cord-276978-xl4u7n6r	41	14	.	.	.
cord-276978-xl4u7n6r	42	1	As	as	IN
cord-276978-xl4u7n6r	42	2	s2	s2	NNP
cord-276978-xl4u7n6r	42	3	m	m	NNP
cord-276978-xl4u7n6r	42	4	is	be	VBZ
cord-276978-xl4u7n6r	42	5	located	locate	VBN
cord-276978-xl4u7n6r	42	6	about	about	IN
cord-276978-xl4u7n6r	42	7	100	100	CD
cord-276978-xl4u7n6r	42	8	-	-	SYM
cord-276978-xl4u7n6r	42	9	150	150	CD
cord-276978-xl4u7n6r	42	10	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	42	11	upstream	upstream	RB
cord-276978-xl4u7n6r	42	12	of	of	IN
cord-276978-xl4u7n6r	42	13	the	the	DT
cord-276978-xl4u7n6r	42	14	poly(A	poly(a	JJ
cord-276978-xl4u7n6r	42	15	)	)	-RRB-
cord-276978-xl4u7n6r	42	16	tail	tail	NN
cord-276978-xl4u7n6r	42	17	of	of	IN
cord-276978-xl4u7n6r	42	18	the	the	DT
cord-276978-xl4u7n6r	42	19	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	42	20	harboring	harbor	VBG
cord-276978-xl4u7n6r	42	21	it	-PRON-	PRP
cord-276978-xl4u7n6r	42	22	,	,	,
cord-276978-xl4u7n6r	42	23	50	50	CD
cord-276978-xl4u7n6r	42	24	-	-	SYM
cord-276978-xl4u7n6r	42	25	100	100	CD
cord-276978-xl4u7n6r	42	26	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	42	27	can	can	MD
cord-276978-xl4u7n6r	42	28	be	be	VB
cord-276978-xl4u7n6r	42	29	determined	determine	VBN
cord-276978-xl4u7n6r	42	30	that	that	DT
cord-276978-xl4u7n6r	42	31	way	way	NN
cord-276978-xl4u7n6r	42	32	,	,	,
cord-276978-xl4u7n6r	42	33	and	and	CC
cord-276978-xl4u7n6r	42	34	two	two	CD
cord-276978-xl4u7n6r	42	35	new	new	JJ
cord-276978-xl4u7n6r	42	36	primers	primer	NNS
cord-276978-xl4u7n6r	42	37	are	be	VBP
cord-276978-xl4u7n6r	42	38	designed	design	VBN
cord-276978-xl4u7n6r	42	39	,	,	,
cord-276978-xl4u7n6r	42	40	which	which	WDT
cord-276978-xl4u7n6r	42	41	can	can	MD
cord-276978-xl4u7n6r	42	42	be	be	VB
cord-276978-xl4u7n6r	42	43	partly	partly	RB
cord-276978-xl4u7n6r	42	44	overlapping	overlap	VBG
cord-276978-xl4u7n6r	42	45	,	,	,
cord-276978-xl4u7n6r	42	46	for	for	IN
cord-276978-xl4u7n6r	42	47	use	use	NN
cord-276978-xl4u7n6r	42	48	in	in	IN
cord-276978-xl4u7n6r	42	49	PCR	PCR	NNP
cord-276978-xl4u7n6r	42	50	and	and	CC
cord-276978-xl4u7n6r	42	51	sequencing	sequencing	NN
cord-276978-xl4u7n6r	42	52	in	in	IN
cord-276978-xl4u7n6r	42	53	the	the	DT
cord-276978-xl4u7n6r	42	54	5	5	CD
cord-276978-xl4u7n6r	42	55	-RACE	-race	NN
cord-276978-xl4u7n6r	42	56	strategy	strategy	NN
cord-276978-xl4u7n6r	42	57	(	(	-LRB-
cord-276978-xl4u7n6r	42	58	see	see	VB
cord-276978-xl4u7n6r	42	59	Note	Note	NNP
cord-276978-xl4u7n6r	42	60	5	5	CD
cord-276978-xl4u7n6r	42	61	)	)	-RRB-
cord-276978-xl4u7n6r	42	62	.	.	.
cord-276978-xl4u7n6r	43	1	A	a	DT
cord-276978-xl4u7n6r	43	2	new	new	JJ
cord-276978-xl4u7n6r	43	3	RT	RT	NNP
cord-276978-xl4u7n6r	43	4	is	be	VBZ
cord-276978-xl4u7n6r	43	5	performed	perform	VBN
cord-276978-xl4u7n6r	43	6	,	,	,
cord-276978-xl4u7n6r	43	7	with	with	IN
cord-276978-xl4u7n6r	43	8	care	care	NN
cord-276978-xl4u7n6r	43	9	taken	take	VBN
cord-276978-xl4u7n6r	43	10	not	not	RB
cord-276978-xl4u7n6r	43	11	to	to	TO
cord-276978-xl4u7n6r	43	12	vortex	vortex	VB
cord-276978-xl4u7n6r	43	13	samples	sample	NNS
cord-276978-xl4u7n6r	43	14	,	,	,
cord-276978-xl4u7n6r	43	15	mixing	mix	VBG
cord-276978-xl4u7n6r	43	16	by	by	IN
cord-276978-xl4u7n6r	43	17	gentle	gentle	JJ
cord-276978-xl4u7n6r	43	18	pipetting	pipetting	NN
cord-276978-xl4u7n6r	43	19	to	to	TO
cord-276978-xl4u7n6r	43	20	avoid	avoid	VB
cord-276978-xl4u7n6r	43	21	breakage	breakage	NN
cord-276978-xl4u7n6r	43	22	of	of	IN
cord-276978-xl4u7n6r	43	23	the	the	DT
cord-276978-xl4u7n6r	43	24	genomic	genomic	JJ
cord-276978-xl4u7n6r	43	25	RNA	rna	NN
cord-276978-xl4u7n6r	43	26	molecule	molecule	NN
cord-276978-xl4u7n6r	43	27	and	and	CC
cord-276978-xl4u7n6r	43	28	achieve	achieve	VB
cord-276978-xl4u7n6r	43	29	long	long	JJ
cord-276978-xl4u7n6r	43	30	-	-	HYPH
cord-276978-xl4u7n6r	43	31	range	range	NN
cord-276978-xl4u7n6r	43	32	cDNA	cdna	NN
cord-276978-xl4u7n6r	43	33	synthesis	synthesis	NN
cord-276978-xl4u7n6r	43	34	,	,	,
cord-276978-xl4u7n6r	43	35	to	to	TO
cord-276978-xl4u7n6r	43	36	get	get	VB
cord-276978-xl4u7n6r	43	37	long	long	JJ
cord-276978-xl4u7n6r	43	38	sequence	sequence	NN
cord-276978-xl4u7n6r	43	39	stretches	stretch	VBZ
cord-276978-xl4u7n6r	43	40	in	in	IN
cord-276978-xl4u7n6r	43	41	the	the	DT
cord-276978-xl4u7n6r	43	42	5	5	CD
cord-276978-xl4u7n6r	43	43	-RACE	-race	NN
cord-276978-xl4u7n6r	43	44	procedure	procedure	NN
cord-276978-xl4u7n6r	43	45	(	(	-LRB-
cord-276978-xl4u7n6r	43	46	see	see	VB
cord-276978-xl4u7n6r	43	47	Note	Note	NNP
cord-276978-xl4u7n6r	43	48	6	6	CD
cord-276978-xl4u7n6r	43	49	)	)	-RRB-
cord-276978-xl4u7n6r	43	50	.	.	.
cord-276978-xl4u7n6r	44	1	A	a	DT
cord-276978-xl4u7n6r	44	2	10-l	10-l	CD
cord-276978-xl4u7n6r	44	3	RNA	RNA	NNP
cord-276978-xl4u7n6r	44	4	extract	extract	NN
cord-276978-xl4u7n6r	44	5	is	be	VBZ
cord-276978-xl4u7n6r	44	6	added	add	VBN
cord-276978-xl4u7n6r	44	7	to	to	IN
cord-276978-xl4u7n6r	44	8	1	1	CD
cord-276978-xl4u7n6r	44	9	l	l	NN
cord-276978-xl4u7n6r	44	10	anchored	anchor	VBD
cord-276978-xl4u7n6r	44	11	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	44	12	)	)	-RRB-
cord-276978-xl4u7n6r	44	13	20	20	CD
cord-276978-xl4u7n6r	44	14	primer	primer	NN
cord-276978-xl4u7n6r	44	15	(	(	-LRB-
cord-276978-xl4u7n6r	44	16	2.5	2.5	CD
cord-276978-xl4u7n6r	44	17	g	g	NN
cord-276978-xl4u7n6r	44	18	/	/	SYM
cord-276978-xl4u7n6r	44	19	l	l	NN
cord-276978-xl4u7n6r	44	20	)	)	-RRB-
cord-276978-xl4u7n6r	44	21	,	,	,
cord-276978-xl4u7n6r	44	22	and	and	CC
cord-276978-xl4u7n6r	44	23	incubated	incubate	VBN
cord-276978-xl4u7n6r	44	24	for	for	IN
cord-276978-xl4u7n6r	44	25	5	5	CD
cord-276978-xl4u7n6r	44	26	min	min	NN
cord-276978-xl4u7n6r	44	27	at	at	IN
cord-276978-xl4u7n6r	44	28	65	65	CD
cord-276978-xl4u7n6r	44	29	•	•	NN
cord-276978-xl4u7n6r	44	30	C	c	NN
cord-276978-xl4u7n6r	44	31	,	,	,
cord-276978-xl4u7n6r	44	32	to	to	TO
cord-276978-xl4u7n6r	44	33	get	get	VB
cord-276978-xl4u7n6r	44	34	rid	rid	VBN
cord-276978-xl4u7n6r	44	35	of	of	IN
cord-276978-xl4u7n6r	44	36	RNA	RNA	NNP
cord-276978-xl4u7n6r	44	37	secondary	secondary	JJ
cord-276978-xl4u7n6r	44	38	structures	structure	NNS
cord-276978-xl4u7n6r	44	39	.	.	.
cord-276978-xl4u7n6r	45	1	The	the	DT
cord-276978-xl4u7n6r	45	2	sample	sample	NN
cord-276978-xl4u7n6r	45	3	is	be	VBZ
cord-276978-xl4u7n6r	45	4	then	then	RB
cord-276978-xl4u7n6r	45	5	rapidly	rapidly	RB
cord-276978-xl4u7n6r	45	6	chilled	chill	VBN
cord-276978-xl4u7n6r	45	7	on	on	IN
cord-276978-xl4u7n6r	45	8	ice	ice	NN
cord-276978-xl4u7n6r	45	9	,	,	,
cord-276978-xl4u7n6r	45	10	for	for	IN
cord-276978-xl4u7n6r	45	11	1	1	CD
cord-276978-xl4u7n6r	45	12	min	min	NN
cord-276978-xl4u7n6r	45	13	.	.	.
cord-276978-xl4u7n6r	46	1	The	the	DT
cord-276978-xl4u7n6r	46	2	sample	sample	NN
cord-276978-xl4u7n6r	46	3	is	be	VBZ
cord-276978-xl4u7n6r	46	4	then	then	RB
cord-276978-xl4u7n6r	46	5	spun	spin	VBN
cord-276978-xl4u7n6r	46	6	down	down	RP
cord-276978-xl4u7n6r	46	7	,	,	,
cord-276978-xl4u7n6r	46	8	and	and	CC
cord-276978-xl4u7n6r	46	9	4	4	CD
cord-276978-xl4u7n6r	46	10	l	l	NN
cord-276978-xl4u7n6r	46	11	5X	5x	CD
cord-276978-xl4u7n6r	46	12	first	first	JJ
cord-276978-xl4u7n6r	46	13	-	-	HYPH
cord-276978-xl4u7n6r	46	14	strand	strand	NN
cord-276978-xl4u7n6r	46	15	buffer	buffer	NN
cord-276978-xl4u7n6r	46	16	,	,	,
cord-276978-xl4u7n6r	46	17	1	1	CD
cord-276978-xl4u7n6r	46	18	l	l	NN
cord-276978-xl4u7n6r	46	19	10	10	CD
cord-276978-xl4u7n6r	46	20	mM	mM	NNP
cord-276978-xl4u7n6r	46	21	dNTP	dntp	NN
cord-276978-xl4u7n6r	46	22	,	,	,
cord-276978-xl4u7n6r	46	23	and	and	CC
cord-276978-xl4u7n6r	46	24	1	1	CD
cord-276978-xl4u7n6r	46	25	l	l	NN
cord-276978-xl4u7n6r	46	26	100	100	CD
cord-276978-xl4u7n6r	46	27	mM	mM	NNP
cord-276978-xl4u7n6r	46	28	DTT	DTT	NNP
cord-276978-xl4u7n6r	46	29	are	be	VBP
cord-276978-xl4u7n6r	46	30	added	add	VBN
cord-276978-xl4u7n6r	46	31	and	and	CC
cord-276978-xl4u7n6r	46	32	mixed	mix	VBN
cord-276978-xl4u7n6r	46	33	gently	gently	RB
cord-276978-xl4u7n6r	46	34	by	by	IN
cord-276978-xl4u7n6r	46	35	pipetting	pipette	VBG
cord-276978-xl4u7n6r	46	36	up	up	RP
cord-276978-xl4u7n6r	46	37	and	and	CC
cord-276978-xl4u7n6r	46	38	down	down	RB
cord-276978-xl4u7n6r	46	39	.	.	.
cord-276978-xl4u7n6r	47	1	The	the	DT
cord-276978-xl4u7n6r	47	2	samples	sample	NNS
cord-276978-xl4u7n6r	47	3	are	be	VBP
cord-276978-xl4u7n6r	47	4	then	then	RB
cord-276978-xl4u7n6r	47	5	incubated	incubate	VBN
cord-276978-xl4u7n6r	47	6	for	for	IN
cord-276978-xl4u7n6r	47	7	1	1	CD
cord-276978-xl4u7n6r	47	8	min	min	NN
cord-276978-xl4u7n6r	47	9	at	at	IN
cord-276978-xl4u7n6r	47	10	55	55	CD
cord-276978-xl4u7n6r	48	1	•	•	NNP
cord-276978-xl4u7n6r	48	2	C	C	NNP
cord-276978-xl4u7n6r	48	3	,	,	,
cord-276978-xl4u7n6r	48	4	and	and	CC
cord-276978-xl4u7n6r	48	5	1	1	CD
cord-276978-xl4u7n6r	48	6	l	l	NN
cord-276978-xl4u7n6r	48	7	RNaseOUT	rnaseout	NN
cord-276978-xl4u7n6r	48	8	(	(	-LRB-
cord-276978-xl4u7n6r	48	9	40	40	CD
cord-276978-xl4u7n6r	48	10	U	U	NNP
cord-276978-xl4u7n6r	48	11	/	/	SYM
cord-276978-xl4u7n6r	48	12	l	l	NN
cord-276978-xl4u7n6r	48	13	)	)	-RRB-
cord-276978-xl4u7n6r	48	14	and	and	CC
cord-276978-xl4u7n6r	48	15	2	2	CD
cord-276978-xl4u7n6r	48	16	l	l	NN
cord-276978-xl4u7n6r	48	17	Superscript	superscript	NN
cord-276978-xl4u7n6r	48	18	III	III	NNP
cord-276978-xl4u7n6r	48	19	(	(	-LRB-
cord-276978-xl4u7n6r	48	20	200	200	CD
cord-276978-xl4u7n6r	48	21	U	u	NN
cord-276978-xl4u7n6r	48	22	/	/	SYM
cord-276978-xl4u7n6r	48	23	l	l	NN
cord-276978-xl4u7n6r	48	24	)	)	-RRB-
cord-276978-xl4u7n6r	48	25	are	be	VBP
cord-276978-xl4u7n6r	48	26	added	add	VBN
cord-276978-xl4u7n6r	48	27	to	to	IN
cord-276978-xl4u7n6r	48	28	each	each	DT
cord-276978-xl4u7n6r	48	29	sample	sample	NN
cord-276978-xl4u7n6r	48	30	,	,	,
cord-276978-xl4u7n6r	48	31	and	and	CC
cord-276978-xl4u7n6r	48	32	the	the	DT
cord-276978-xl4u7n6r	48	33	samples	sample	NNS
cord-276978-xl4u7n6r	48	34	are	be	VBP
cord-276978-xl4u7n6r	48	35	rapidly	rapidly	RB
cord-276978-xl4u7n6r	48	36	put	put	VBN
cord-276978-xl4u7n6r	48	37	back	back	RB
cord-276978-xl4u7n6r	48	38	into	into	IN
cord-276978-xl4u7n6r	48	39	the	the	DT
cord-276978-xl4u7n6r	48	40	thermocycler	thermocycler	NN
cord-276978-xl4u7n6r	48	41	used	use	VBN
cord-276978-xl4u7n6r	48	42	for	for	IN
cord-276978-xl4u7n6r	48	43	RT	RT	NNP
cord-276978-xl4u7n6r	48	44	.	.	.
cord-276978-xl4u7n6r	49	1	The	the	DT
cord-276978-xl4u7n6r	49	2	RT	RT	NNP
cord-276978-xl4u7n6r	49	3	is	be	VBZ
cord-276978-xl4u7n6r	49	4	performed	perform	VBN
cord-276978-xl4u7n6r	49	5	at	at	IN
cord-276978-xl4u7n6r	49	6	55	55	CD
cord-276978-xl4u7n6r	49	7	•	•	NN
cord-276978-xl4u7n6r	49	8	C	C	NNP
cord-276978-xl4u7n6r	49	9	,	,	,
cord-276978-xl4u7n6r	49	10	for	for	IN
cord-276978-xl4u7n6r	49	11	80	80	CD
cord-276978-xl4u7n6r	49	12	min	min	NN
cord-276978-xl4u7n6r	49	13	,	,	,
cord-276978-xl4u7n6r	49	14	followed	follow	VBN
cord-276978-xl4u7n6r	49	15	by	by	IN
cord-276978-xl4u7n6r	49	16	enzyme	enzyme	NN
cord-276978-xl4u7n6r	49	17	inactivation	inactivation	NN
cord-276978-xl4u7n6r	49	18	at	at	IN
cord-276978-xl4u7n6r	49	19	70	70	CD
cord-276978-xl4u7n6r	50	1	•	•	NNP
cord-276978-xl4u7n6r	50	2	C	C	NNP
cord-276978-xl4u7n6r	50	3	and	and	CC
cord-276978-xl4u7n6r	50	4	cooling	cool	VBG
cord-276978-xl4u7n6r	50	5	at	at	IN
cord-276978-xl4u7n6r	51	1	4	4	LS
cord-276978-xl4u7n6r	51	2	•	•	NN
cord-276978-xl4u7n6r	51	3	C	C	NNP
cord-276978-xl4u7n6r	51	4	thereafter	thereafter	RB
cord-276978-xl4u7n6r	51	5	(	(	-LRB-
cord-276978-xl4u7n6r	51	6	see	see	VB
cord-276978-xl4u7n6r	51	7	Note	Note	NNP
cord-276978-xl4u7n6r	51	8	7	7	CD
cord-276978-xl4u7n6r	51	9	)	)	-RRB-
cord-276978-xl4u7n6r	51	10	.	.	.
cord-276978-xl4u7n6r	52	1	2	2	LS
cord-276978-xl4u7n6r	52	2	.	.	.
cord-276978-xl4u7n6r	53	1	The	the	DT
cord-276978-xl4u7n6r	53	2	samples	sample	NNS
cord-276978-xl4u7n6r	53	3	from	from	IN
cord-276978-xl4u7n6r	53	4	reverse	reverse	JJ
cord-276978-xl4u7n6r	53	5	transcription	transcription	NN
cord-276978-xl4u7n6r	53	6	are	be	VBP
cord-276978-xl4u7n6r	53	7	centrifuged	centrifuge	VBN
cord-276978-xl4u7n6r	53	8	for	for	IN
cord-276978-xl4u7n6r	53	9	20	20	CD
cord-276978-xl4u7n6r	53	10	sec	sec	NNS
cord-276978-xl4u7n6r	53	11	at	at	IN
cord-276978-xl4u7n6r	53	12	1538	1538	CD
cord-276978-xl4u7n6r	53	13	×	×	NNP
cord-276978-xl4u7n6r	53	14	g	g	NNP
cord-276978-xl4u7n6r	53	15	to	to	TO
cord-276978-xl4u7n6r	53	16	collect	collect	VB
cord-276978-xl4u7n6r	53	17	all	all	DT
cord-276978-xl4u7n6r	53	18	droplets	droplet	NNS
cord-276978-xl4u7n6r	53	19	,	,	,
cord-276978-xl4u7n6r	53	20	and	and	CC
cord-276978-xl4u7n6r	53	21	incubated	incubate	VBN
cord-276978-xl4u7n6r	53	22	at	at	IN
cord-276978-xl4u7n6r	53	23	37	37	CD
cord-276978-xl4u7n6r	53	24	•	•	NN
cord-276978-xl4u7n6r	53	25	C	c	NN
cord-276978-xl4u7n6r	53	26	in	in	IN
cord-276978-xl4u7n6r	53	27	a	a	DT
cord-276978-xl4u7n6r	53	28	thermocycler	thermocycler	NN
cord-276978-xl4u7n6r	53	29	.	.	.
cord-276978-xl4u7n6r	54	1	A	a	DT
cord-276978-xl4u7n6r	54	2	1-l	1-l	CD
cord-276978-xl4u7n6r	54	3	RNase	rnase	NN
cord-276978-xl4u7n6r	54	4	mix	mix	NN
cord-276978-xl4u7n6r	54	5	is	be	VBZ
cord-276978-xl4u7n6r	54	6	then	then	RB
cord-276978-xl4u7n6r	54	7	added	add	VBN
cord-276978-xl4u7n6r	54	8	to	to	IN
cord-276978-xl4u7n6r	54	9	each	each	DT
cord-276978-xl4u7n6r	54	10	sample	sample	NN
cord-276978-xl4u7n6r	54	11	(	(	-LRB-
cord-276978-xl4u7n6r	54	12	see	see	VB
cord-276978-xl4u7n6r	54	13	Note	note	NN
cord-276978-xl4u7n6r	54	14	8)	8)	NN
cord-276978-xl4u7n6r	54	15	and	and	CC
cord-276978-xl4u7n6r	54	16	mixed	mix	VBN
cord-276978-xl4u7n6r	54	17	gently	gently	RB
cord-276978-xl4u7n6r	54	18	by	by	IN
cord-276978-xl4u7n6r	54	19	pipetting	pipette	VBG
cord-276978-xl4u7n6r	54	20	up	up	RP
cord-276978-xl4u7n6r	54	21	and	and	CC
cord-276978-xl4u7n6r	54	22	down	down	RB
cord-276978-xl4u7n6r	54	23	,	,	,
cord-276978-xl4u7n6r	54	24	and	and	CC
cord-276978-xl4u7n6r	54	25	the	the	DT
cord-276978-xl4u7n6r	54	26	samples	sample	NNS
cord-276978-xl4u7n6r	54	27	are	be	VBP
cord-276978-xl4u7n6r	54	28	put	put	VBN
cord-276978-xl4u7n6r	54	29	back	back	RB
cord-276978-xl4u7n6r	54	30	into	into	IN
cord-276978-xl4u7n6r	54	31	the	the	DT
cord-276978-xl4u7n6r	54	32	thermocycler	thermocycler	NN
cord-276978-xl4u7n6r	54	33	for	for	IN
cord-276978-xl4u7n6r	54	34	further	further	JJ
cord-276978-xl4u7n6r	54	35	incubation	incubation	NN
cord-276978-xl4u7n6r	54	36	for	for	IN
cord-276978-xl4u7n6r	54	37	30	30	CD
cord-276978-xl4u7n6r	54	38	min	min	NN
cord-276978-xl4u7n6r	54	39	at	at	IN
cord-276978-xl4u7n6r	54	40	37	37	CD
cord-276978-xl4u7n6r	54	41	•	•	NN
cord-276978-xl4u7n6r	54	42	C.	c.	NN
cord-276978-xl4u7n6r	55	1	When	when	WRB
cord-276978-xl4u7n6r	55	2	the	the	DT
cord-276978-xl4u7n6r	55	3	incubation	incubation	NN
cord-276978-xl4u7n6r	55	4	is	be	VBZ
cord-276978-xl4u7n6r	55	5	finished	finish	VBN
cord-276978-xl4u7n6r	55	6	,	,	,
cord-276978-xl4u7n6r	55	7	the	the	DT
cord-276978-xl4u7n6r	55	8	samples	sample	NNS
cord-276978-xl4u7n6r	55	9	are	be	VBP
cord-276978-xl4u7n6r	55	10	spun	spin	VBN
cord-276978-xl4u7n6r	55	11	down	down	RP
cord-276978-xl4u7n6r	55	12	and	and	CC
cord-276978-xl4u7n6r	55	13	cooled	cool	VBN
cord-276978-xl4u7n6r	55	14	.	.	.
cord-276978-xl4u7n6r	56	1	3	3	LS
cord-276978-xl4u7n6r	56	2	.	.	.
cord-276978-xl4u7n6r	57	1	The	the	DT
cord-276978-xl4u7n6r	57	2	newly	newly	RB
cord-276978-xl4u7n6r	57	3	synthesized	synthesize	VBN
cord-276978-xl4u7n6r	57	4	cDNA	cdna	NN
cord-276978-xl4u7n6r	57	5	is	be	VBZ
cord-276978-xl4u7n6r	57	6	purified	purify	VBN
cord-276978-xl4u7n6r	57	7	using	use	VBG
cord-276978-xl4u7n6r	57	8	the	the	DT
cord-276978-xl4u7n6r	57	9	QIAquick	QIAquick	NNP
cord-276978-xl4u7n6r	57	10	PCR	PCR	NNP
cord-276978-xl4u7n6r	57	11	purification	purification	NN
cord-276978-xl4u7n6r	57	12	kit	kit	NN
cord-276978-xl4u7n6r	57	13	according	accord	VBG
cord-276978-xl4u7n6r	57	14	to	to	IN
cord-276978-xl4u7n6r	57	15	the	the	DT
cord-276978-xl4u7n6r	57	16	manufacturer	manufacturer	NN
cord-276978-xl4u7n6r	57	17	's	's	POS
cord-276978-xl4u7n6r	57	18	instructions	instruction	NNS
cord-276978-xl4u7n6r	57	19	,	,	,
cord-276978-xl4u7n6r	57	20	with	with	IN
cord-276978-xl4u7n6r	57	21	the	the	DT
cord-276978-xl4u7n6r	57	22	following	following	JJ
cord-276978-xl4u7n6r	57	23	modifications	modification	NNS
cord-276978-xl4u7n6r	57	24	:	:	:
cord-276978-xl4u7n6r	57	25	all	all	DT
cord-276978-xl4u7n6r	57	26	mixing	mix	VBG
cord-276978-xl4u7n6r	57	27	steps	step	NNS
cord-276978-xl4u7n6r	57	28	are	be	VBP
cord-276978-xl4u7n6r	57	29	performed	perform	VBN
cord-276978-xl4u7n6r	57	30	gently	gently	RB
cord-276978-xl4u7n6r	57	31	,	,	,
cord-276978-xl4u7n6r	57	32	and	and	CC
cord-276978-xl4u7n6r	57	33	the	the	DT
cord-276978-xl4u7n6r	57	34	sample	sample	NN
cord-276978-xl4u7n6r	57	35	is	be	VBZ
cord-276978-xl4u7n6r	57	36	eluted	elute	VBN
cord-276978-xl4u7n6r	57	37	in	in	IN
cord-276978-xl4u7n6r	57	38	30	30	CD
cord-276978-xl4u7n6r	57	39	l	l	NN
cord-276978-xl4u7n6r	57	40	RNase	RNase	NNP
cord-276978-xl4u7n6r	57	41	/	/	SYM
cord-276978-xl4u7n6r	57	42	DNase	DNase	NNP
cord-276978-xl4u7n6r	57	43	-	-	HYPH
cord-276978-xl4u7n6r	57	44	free	free	JJ
cord-276978-xl4u7n6r	57	45	water	water	NN
cord-276978-xl4u7n6r	57	46	prewarmed	prewarme	VBN
cord-276978-xl4u7n6r	57	47	at	at	IN
cord-276978-xl4u7n6r	57	48	65	65	CD
cord-276978-xl4u7n6r	58	1	•	•	NNP
cord-276978-xl4u7n6r	58	2	C.	C.	NNP
cord-276978-xl4u7n6r	59	1	The	the	DT
cord-276978-xl4u7n6r	59	2	cDNA	cdna	NN
cord-276978-xl4u7n6r	59	3	amount	amount	NN
cord-276978-xl4u7n6r	59	4	is	be	VBZ
cord-276978-xl4u7n6r	59	5	measured	measure	VBN
cord-276978-xl4u7n6r	59	6	spectrophotometrically	spectrophotometrically	RB
cord-276978-xl4u7n6r	59	7	on	on	IN
cord-276978-xl4u7n6r	59	8	a	a	DT
cord-276978-xl4u7n6r	59	9	NanoDrop	NanoDrop	NNP
cord-276978-xl4u7n6r	59	10	.	.	.
cord-276978-xl4u7n6r	60	1	4	4	LS
cord-276978-xl4u7n6r	60	2	.	.	.
cord-276978-xl4u7n6r	61	1	Cytosine	cytosine	NN
cord-276978-xl4u7n6r	61	2	residues	residue	NNS
cord-276978-xl4u7n6r	61	3	are	be	VBP
cord-276978-xl4u7n6r	61	4	added	add	VBN
cord-276978-xl4u7n6r	61	5	to	to	IN
cord-276978-xl4u7n6r	61	6	the	the	DT
cord-276978-xl4u7n6r	61	7	3'-ends	3'-ends	CD
cord-276978-xl4u7n6r	61	8	of	of	IN
cord-276978-xl4u7n6r	61	9	purified	purify	VBN
cord-276978-xl4u7n6r	61	10	cDNA	cdna	NN
cord-276978-xl4u7n6r	61	11	,	,	,
cord-276978-xl4u7n6r	61	12	in	in	IN
cord-276978-xl4u7n6r	61	13	order	order	NN
cord-276978-xl4u7n6r	61	14	to	to	TO
cord-276978-xl4u7n6r	61	15	make	make	VB
cord-276978-xl4u7n6r	61	16	a	a	DT
cord-276978-xl4u7n6r	61	17	homopolymeric	homopolymeric	NN
cord-276978-xl4u7n6r	61	18	(	(	-LRB-
cord-276978-xl4u7n6r	61	19	C	c	NN
cord-276978-xl4u7n6r	61	20	)	)	-RRB-
cord-276978-xl4u7n6r	61	21	tail	tail	NN
cord-276978-xl4u7n6r	61	22	to	to	TO
cord-276978-xl4u7n6r	61	23	serve	serve	VB
cord-276978-xl4u7n6r	61	24	as	as	IN
cord-276978-xl4u7n6r	61	25	a	a	DT
cord-276978-xl4u7n6r	61	26	handle	handle	NN
cord-276978-xl4u7n6r	61	27	for	for	IN
cord-276978-xl4u7n6r	61	28	semispecific	semispecific	NN
cord-276978-xl4u7n6r	61	29	amplification	amplification	NN
cord-276978-xl4u7n6r	61	30	with	with	IN
cord-276978-xl4u7n6r	61	31	a	a	DT
cord-276978-xl4u7n6r	61	32	specially	specially	RB
cord-276978-xl4u7n6r	61	33	designed	design	VBN
cord-276978-xl4u7n6r	61	34	anchor	anchor	NN
cord-276978-xl4u7n6r	61	35	primer	primer	NN
cord-276978-xl4u7n6r	61	36	.	.	.
cord-276978-xl4u7n6r	62	1	The	the	DT
cord-276978-xl4u7n6r	62	2	tailing	tailing	NN
cord-276978-xl4u7n6r	62	3	is	be	VBZ
cord-276978-xl4u7n6r	62	4	performed	perform	VBN
cord-276978-xl4u7n6r	62	5	as	as	IN
cord-276978-xl4u7n6r	62	6	follows	follow	VBZ
cord-276978-xl4u7n6r	62	7	:	:	:
cord-276978-xl4u7n6r	62	8	10	10	CD
cord-276978-xl4u7n6r	62	9	l	l	NN
cord-276978-xl4u7n6r	62	10	of	of	IN
cord-276978-xl4u7n6r	62	11	the	the	DT
cord-276978-xl4u7n6r	62	12	purified	purify	VBN
cord-276978-xl4u7n6r	62	13	cDNA	cdna	NN
cord-276978-xl4u7n6r	62	14	is	be	VBZ
cord-276978-xl4u7n6r	62	15	added	add	VBN
cord-276978-xl4u7n6r	62	16	to	to	IN
cord-276978-xl4u7n6r	62	17	6.5	6.5	CD
cord-276978-xl4u7n6r	62	18	l	l	NN
cord-276978-xl4u7n6r	62	19	RNase	RNase	NNP
cord-276978-xl4u7n6r	62	20	/	/	SYM
cord-276978-xl4u7n6r	62	21	DNase	DNase	NNP
cord-276978-xl4u7n6r	62	22	-	-	HYPH
cord-276978-xl4u7n6r	62	23	free	free	JJ
cord-276978-xl4u7n6r	62	24	water	water	NN
cord-276978-xl4u7n6r	62	25	,	,	,
cord-276978-xl4u7n6r	62	26	5	5	CD
cord-276978-xl4u7n6r	62	27	l	l	NN
cord-276978-xl4u7n6r	62	28	5X	5x	CD
cord-276978-xl4u7n6r	62	29	tailing	tailing	NN
cord-276978-xl4u7n6r	62	30	buffer	buffer	NN
cord-276978-xl4u7n6r	62	31	,	,	,
cord-276978-xl4u7n6r	62	32	and	and	CC
cord-276978-xl4u7n6r	62	33	2.5	2.5	CD
cord-276978-xl4u7n6r	62	34	l	l	NN
cord-276978-xl4u7n6r	62	35	2	2	CD
cord-276978-xl4u7n6r	62	36	mM	mm	NN
cord-276978-xl4u7n6r	62	37	dCTP	dctp	NN
cord-276978-xl4u7n6r	62	38	.	.	.
cord-276978-xl4u7n6r	63	1	In	in	IN
cord-276978-xl4u7n6r	63	2	order	order	NN
cord-276978-xl4u7n6r	63	3	to	to	TO
cord-276978-xl4u7n6r	63	4	avoid	avoid	VB
cord-276978-xl4u7n6r	63	5	secondary	secondary	JJ
cord-276978-xl4u7n6r	63	6	structure	structure	NN
cord-276978-xl4u7n6r	63	7	in	in	IN
cord-276978-xl4u7n6r	63	8	the	the	DT
cord-276978-xl4u7n6r	63	9	cDNA	cdna	NN
cord-276978-xl4u7n6r	63	10	that	that	WDT
cord-276978-xl4u7n6r	63	11	might	may	MD
cord-276978-xl4u7n6r	63	12	impair	impair	VB
cord-276978-xl4u7n6r	63	13	the	the	DT
cord-276978-xl4u7n6r	63	14	tailing	tailing	NN
cord-276978-xl4u7n6r	63	15	,	,	,
cord-276978-xl4u7n6r	63	16	a	a	DT
cord-276978-xl4u7n6r	63	17	short	short	JJ
cord-276978-xl4u7n6r	63	18	denaturation	denaturation	NN
cord-276978-xl4u7n6r	63	19	step	step	NN
cord-276978-xl4u7n6r	63	20	is	be	VBZ
cord-276978-xl4u7n6r	63	21	performed	perform	VBN
cord-276978-xl4u7n6r	63	22	at	at	IN
cord-276978-xl4u7n6r	63	23	94	94	CD
cord-276978-xl4u7n6r	64	1	•	•	NNP
cord-276978-xl4u7n6r	64	2	C	C	NNP
cord-276978-xl4u7n6r	64	3	for	for	IN
cord-276978-xl4u7n6r	64	4	3	3	CD
cord-276978-xl4u7n6r	64	5	min	min	NN
cord-276978-xl4u7n6r	64	6	.	.	.
cord-276978-xl4u7n6r	65	1	The	the	DT
cord-276978-xl4u7n6r	65	2	sample	sample	NN
cord-276978-xl4u7n6r	65	3	is	be	VBZ
cord-276978-xl4u7n6r	65	4	then	then	RB
cord-276978-xl4u7n6r	65	5	immediately	immediately	RB
cord-276978-xl4u7n6r	65	6	chilled	chill	VBN
cord-276978-xl4u7n6r	65	7	on	on	IN
cord-276978-xl4u7n6r	65	8	ice	ice	NN
cord-276978-xl4u7n6r	65	9	and	and	CC
cord-276978-xl4u7n6r	65	10	spun	spin	VBD
cord-276978-xl4u7n6r	65	11	down	down	RP
cord-276978-xl4u7n6r	65	12	.	.	.
cord-276978-xl4u7n6r	66	1	One	one	CD
cord-276978-xl4u7n6r	66	2	l	l	NN
cord-276978-xl4u7n6r	66	3	TdT	tdt	NN
cord-276978-xl4u7n6r	66	4	(	(	-LRB-
cord-276978-xl4u7n6r	66	5	15	15	CD
cord-276978-xl4u7n6r	66	6	U	U	NNP
cord-276978-xl4u7n6r	66	7	/	/	SYM
cord-276978-xl4u7n6r	66	8	l	l	NN
cord-276978-xl4u7n6r	66	9	)	)	-RRB-
cord-276978-xl4u7n6r	66	10	is	be	VBZ
cord-276978-xl4u7n6r	66	11	then	then	RB
cord-276978-xl4u7n6r	66	12	added	add	VBN
cord-276978-xl4u7n6r	66	13	,	,	,
cord-276978-xl4u7n6r	66	14	and	and	CC
cord-276978-xl4u7n6r	66	15	the	the	DT
cord-276978-xl4u7n6r	66	16	tailing	tail	VBG
cord-276978-xl4u7n6r	66	17	reaction	reaction	NN
cord-276978-xl4u7n6r	66	18	is	be	VBZ
cord-276978-xl4u7n6r	66	19	performed	perform	VBN
cord-276978-xl4u7n6r	66	20	at	at	IN
cord-276978-xl4u7n6r	66	21	37	37	CD
cord-276978-xl4u7n6r	66	22	•	•	NN
cord-276978-xl4u7n6r	66	23	C	C	NNP
cord-276978-xl4u7n6r	66	24	for	for	IN
cord-276978-xl4u7n6r	66	25	10	10	CD
cord-276978-xl4u7n6r	66	26	min	min	NN
cord-276978-xl4u7n6r	66	27	,	,	,
cord-276978-xl4u7n6r	66	28	followed	follow	VBN
cord-276978-xl4u7n6r	66	29	by	by	IN
cord-276978-xl4u7n6r	66	30	an	an	DT
cord-276978-xl4u7n6r	66	31	enzyme	enzyme	NN
cord-276978-xl4u7n6r	66	32	inactivation	inactivation	NN
cord-276978-xl4u7n6r	66	33	step	step	NN
cord-276978-xl4u7n6r	66	34	at	at	IN
cord-276978-xl4u7n6r	66	35	65	65	CD
cord-276978-xl4u7n6r	66	36	•	•	NN
cord-276978-xl4u7n6r	66	37	C	c	NN
cord-276978-xl4u7n6r	66	38	for	for	IN
cord-276978-xl4u7n6r	66	39	10	10	CD
cord-276978-xl4u7n6r	66	40	min	min	NN
cord-276978-xl4u7n6r	66	41	.	.	.
cord-276978-xl4u7n6r	67	1	The	the	DT
cord-276978-xl4u7n6r	67	2	sample	sample	NN
cord-276978-xl4u7n6r	67	3	is	be	VBZ
cord-276978-xl4u7n6r	67	4	then	then	RB
cord-276978-xl4u7n6r	67	5	spun	spin	VBN
cord-276978-xl4u7n6r	67	6	down	down	RP
cord-276978-xl4u7n6r	67	7	and	and	CC
cord-276978-xl4u7n6r	67	8	cooled	cool	VBD
cord-276978-xl4u7n6r	67	9	on	on	IN
cord-276978-xl4u7n6r	67	10	ice	ice	NN
cord-276978-xl4u7n6r	67	11	.	.	.
cord-276978-xl4u7n6r	68	1	5	5	LS
cord-276978-xl4u7n6r	68	2	.	.	.
cord-276978-xl4u7n6r	69	1	The	the	DT
cord-276978-xl4u7n6r	69	2	5	5	CD
cord-276978-xl4u7n6r	69	3	-RACE	-race	NN
cord-276978-xl4u7n6r	69	4	PCR	PCR	NNP
cord-276978-xl4u7n6r	69	5	is	be	VBZ
cord-276978-xl4u7n6r	69	6	performed	perform	VBN
cord-276978-xl4u7n6r	69	7	as	as	IN
cord-276978-xl4u7n6r	69	8	follows	follow	VBZ
cord-276978-xl4u7n6r	69	9	(	(	-LRB-
cord-276978-xl4u7n6r	69	10	see	see	VB
cord-276978-xl4u7n6r	69	11	Note	Note	NNP
cord-276978-xl4u7n6r	69	12	9	9	CD
cord-276978-xl4u7n6r	69	13	)	)	-RRB-
cord-276978-xl4u7n6r	69	14	:	:	:
cord-276978-xl4u7n6r	69	15	5	5	CD
cord-276978-xl4u7n6r	69	16	l	l	NN
cord-276978-xl4u7n6r	69	17	tailed	tailed	JJ
cord-276978-xl4u7n6r	69	18	sample	sample	NN
cord-276978-xl4u7n6r	69	19	is	be	VBZ
cord-276978-xl4u7n6r	69	20	added	add	VBN
cord-276978-xl4u7n6r	69	21	to	to	IN
cord-276978-xl4u7n6r	69	22	45	45	CD
cord-276978-xl4u7n6r	69	23	l	l	NN
cord-276978-xl4u7n6r	69	24	PCR	PCR	NNP
cord-276978-xl4u7n6r	69	25	mix	mix	NN
cord-276978-xl4u7n6r	69	26	consisting	consist	VBG
cord-276978-xl4u7n6r	69	27	of	of	IN
cord-276978-xl4u7n6r	69	28	34.5	34.5	CD
cord-276978-xl4u7n6r	69	29	l	l	NN
cord-276978-xl4u7n6r	69	30	RNase	RNase	NNP
cord-276978-xl4u7n6r	69	31	/	/	SYM
cord-276978-xl4u7n6r	69	32	DNase	DNase	NNP
cord-276978-xl4u7n6r	69	33	-	-	HYPH
cord-276978-xl4u7n6r	69	34	free	free	JJ
cord-276978-xl4u7n6r	69	35	water	water	NN
cord-276978-xl4u7n6r	69	36	,	,	,
cord-276978-xl4u7n6r	69	37	5	5	CD
cord-276978-xl4u7n6r	69	38	l	l	NN
cord-276978-xl4u7n6r	69	39	10X	10x	CD
cord-276978-xl4u7n6r	69	40	BD	BD	NNP
cord-276978-xl4u7n6r	69	41	Advantage	Advantage	NNP
cord-276978-xl4u7n6r	69	42	2	2	CD
cord-276978-xl4u7n6r	69	43	PCR	PCR	NNP
cord-276978-xl4u7n6r	69	44	buffer	buffer	NN
cord-276978-xl4u7n6r	69	45	,	,	,
cord-276978-xl4u7n6r	69	46	1	1	CD
cord-276978-xl4u7n6r	69	47	l	l	NN
cord-276978-xl4u7n6r	69	48	dNTP	dNTP	NNP
cord-276978-xl4u7n6r	69	49	(	(	-LRB-
cord-276978-xl4u7n6r	69	50	10	10	CD
cord-276978-xl4u7n6r	69	51	mM	mm	CD
cord-276978-xl4u7n6r	69	52	)	)	-RRB-
cord-276978-xl4u7n6r	69	53	,	,	,
cord-276978-xl4u7n6r	69	54	1	1	CD
cord-276978-xl4u7n6r	69	55	l	l	NN
cord-276978-xl4u7n6r	69	56	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	69	57	-	-	HYPH
cord-276978-xl4u7n6r	69	58	specific	specific	JJ
cord-276978-xl4u7n6r	69	59	reverse	reverse	NN
cord-276978-xl4u7n6r	69	60	primer	primer	NN
cord-276978-xl4u7n6r	69	61	(	(	-LRB-
cord-276978-xl4u7n6r	69	62	25	25	CD
cord-276978-xl4u7n6r	69	63	M	M	NNP
cord-276978-xl4u7n6r	69	64	)	)	-RRB-
cord-276978-xl4u7n6r	69	65	,	,	,
cord-276978-xl4u7n6r	69	66	2.5	2.5	CD
cord-276978-xl4u7n6r	69	67	l	l	NN
cord-276978-xl4u7n6r	69	68	abridged	abridge	VBN
cord-276978-xl4u7n6r	69	69	anchor	anchor	NN
cord-276978-xl4u7n6r	69	70	primer	primer	NN
cord-276978-xl4u7n6r	69	71	(	(	-LRB-
cord-276978-xl4u7n6r	69	72	10	10	CD
cord-276978-xl4u7n6r	69	73	M	M	NNP
cord-276978-xl4u7n6r	69	74	)	)	-RRB-
cord-276978-xl4u7n6r	69	75	,	,	,
cord-276978-xl4u7n6r	69	76	and	and	CC
cord-276978-xl4u7n6r	69	77	1	1	CD
cord-276978-xl4u7n6r	69	78	l	l	NN
cord-276978-xl4u7n6r	69	79	BD	BD	NNP
cord-276978-xl4u7n6r	69	80	Advantage	Advantage	NNP
cord-276978-xl4u7n6r	69	81	polymerase	polymerase	NN
cord-276978-xl4u7n6r	69	82	mix	mix	NN
cord-276978-xl4u7n6r	69	83	.	.	.
cord-276978-xl4u7n6r	70	1	The	the	DT
cord-276978-xl4u7n6r	70	2	cycling	cycling	NN
cord-276978-xl4u7n6r	70	3	conditions	condition	NNS
cord-276978-xl4u7n6r	70	4	include	include	VBP
cord-276978-xl4u7n6r	70	5	an	an	DT
cord-276978-xl4u7n6r	70	6	initial	initial	JJ
cord-276978-xl4u7n6r	70	7	enzymeactivation	enzymeactivation	NN
cord-276978-xl4u7n6r	70	8	step	step	NN
cord-276978-xl4u7n6r	70	9	at	at	IN
cord-276978-xl4u7n6r	70	10	95	95	CD
cord-276978-xl4u7n6r	71	1	•	•	NNP
cord-276978-xl4u7n6r	72	1	C	C	NNP
cord-276978-xl4u7n6r	72	2	for	for	IN
cord-276978-xl4u7n6r	72	3	1	1	CD
cord-276978-xl4u7n6r	72	4	min	min	NN
cord-276978-xl4u7n6r	72	5	,	,	,
cord-276978-xl4u7n6r	72	6	followed	follow	VBN
cord-276978-xl4u7n6r	72	7	by	by	IN
cord-276978-xl4u7n6r	72	8	35	35	CD
cord-276978-xl4u7n6r	72	9	cycles	cycle	NNS
cord-276978-xl4u7n6r	72	10	of	of	IN
cord-276978-xl4u7n6r	72	11	94	94	CD
cord-276978-xl4u7n6r	73	1	•	•	NNP
cord-276978-xl4u7n6r	73	2	C	C	NNP
cord-276978-xl4u7n6r	73	3	for	for	IN
cord-276978-xl4u7n6r	73	4	30	30	CD
cord-276978-xl4u7n6r	73	5	sec	sec	NNP
cord-276978-xl4u7n6r	73	6	,	,	,
cord-276978-xl4u7n6r	74	1	55	55	CD
cord-276978-xl4u7n6r	74	2	•	•	NN
cord-276978-xl4u7n6r	74	3	C	c	NN
cord-276978-xl4u7n6r	74	4	for	for	IN
cord-276978-xl4u7n6r	74	5	20	20	CD
cord-276978-xl4u7n6r	74	6	sec	sec	NNP
cord-276978-xl4u7n6r	74	7	,	,	,
cord-276978-xl4u7n6r	74	8	and	and	CC
cord-276978-xl4u7n6r	74	9	68	68	CD
cord-276978-xl4u7n6r	75	1	•	•	NNP
cord-276978-xl4u7n6r	76	1	C	C	NNP
cord-276978-xl4u7n6r	76	2	for	for	IN
cord-276978-xl4u7n6r	76	3	7	7	CD
cord-276978-xl4u7n6r	76	4	min	min	NN
cord-276978-xl4u7n6r	76	5	(	(	-LRB-
cord-276978-xl4u7n6r	76	6	see	see	VB
cord-276978-xl4u7n6r	77	1	Note	note	VB
cord-276978-xl4u7n6r	77	2	10	10	CD
cord-276978-xl4u7n6r	77	3	)	)	-RRB-
cord-276978-xl4u7n6r	77	4	.	.	.
cord-276978-xl4u7n6r	78	1	The	the	DT
cord-276978-xl4u7n6r	78	2	cycling	cycling	NN
cord-276978-xl4u7n6r	78	3	is	be	VBZ
cord-276978-xl4u7n6r	78	4	followed	follow	VBN
cord-276978-xl4u7n6r	78	5	by	by	IN
cord-276978-xl4u7n6r	78	6	a	a	DT
cord-276978-xl4u7n6r	78	7	step	step	NN
cord-276978-xl4u7n6r	78	8	at	at	IN
cord-276978-xl4u7n6r	78	9	68	68	CD
cord-276978-xl4u7n6r	79	1	•	•	NNP
cord-276978-xl4u7n6r	79	2	C	C	NNP
cord-276978-xl4u7n6r	79	3	for	for	IN
cord-276978-xl4u7n6r	79	4	7	7	CD
cord-276978-xl4u7n6r	79	5	min	min	NN
cord-276978-xl4u7n6r	79	6	and	and	CC
cord-276978-xl4u7n6r	79	7	cooling	cool	VBG
cord-276978-xl4u7n6r	79	8	to	to	IN
cord-276978-xl4u7n6r	79	9	4	4	CD
cord-276978-xl4u7n6r	79	10	•	•	NN
cord-276978-xl4u7n6r	79	11	C	c	NN
cord-276978-xl4u7n6r	79	12	thereafter	thereafter	RB
cord-276978-xl4u7n6r	79	13	.	.	.
cord-276978-xl4u7n6r	80	1	6	6	LS
cord-276978-xl4u7n6r	80	2	.	.	.
cord-276978-xl4u7n6r	81	1	Prior	prior	RB
cord-276978-xl4u7n6r	81	2	to	to	IN
cord-276978-xl4u7n6r	81	3	sequencing	sequencing	NN
cord-276978-xl4u7n6r	81	4	reaction	reaction	NN
cord-276978-xl4u7n6r	81	5	,	,	,
cord-276978-xl4u7n6r	81	6	the	the	DT
cord-276978-xl4u7n6r	81	7	PCR	PCR	NNP
cord-276978-xl4u7n6r	81	8	product	product	NN
cord-276978-xl4u7n6r	81	9	,	,	,
cord-276978-xl4u7n6r	81	10	appearing	appear	VBG
cord-276978-xl4u7n6r	81	11	as	as	IN
cord-276978-xl4u7n6r	81	12	a	a	DT
cord-276978-xl4u7n6r	81	13	weak	weak	JJ
cord-276978-xl4u7n6r	81	14	smear	smear	NN
cord-276978-xl4u7n6r	81	15	on	on	IN
cord-276978-xl4u7n6r	81	16	a	a	DT
cord-276978-xl4u7n6r	81	17	1	1	CD
cord-276978-xl4u7n6r	81	18	%	%	NN
cord-276978-xl4u7n6r	81	19	agarose	agarose	NN
cord-276978-xl4u7n6r	81	20	gel	gel	NN
cord-276978-xl4u7n6r	81	21	electrophoresis	electrophoresis	NN
cord-276978-xl4u7n6r	81	22	,	,	,
cord-276978-xl4u7n6r	81	23	is	be	VBZ
cord-276978-xl4u7n6r	81	24	purified	purify	VBN
cord-276978-xl4u7n6r	81	25	directly	directly	RB
cord-276978-xl4u7n6r	81	26	using	use	VBG
cord-276978-xl4u7n6r	81	27	the	the	DT
cord-276978-xl4u7n6r	81	28	QIAquick	QIAquick	NNP
cord-276978-xl4u7n6r	81	29	PCR	PCR	NNP
cord-276978-xl4u7n6r	81	30	purification	purification	NN
cord-276978-xl4u7n6r	81	31	kit	kit	NN
cord-276978-xl4u7n6r	81	32	according	accord	VBG
cord-276978-xl4u7n6r	81	33	to	to	IN
cord-276978-xl4u7n6r	81	34	the	the	DT
cord-276978-xl4u7n6r	81	35	manufacturer	manufacturer	NN
cord-276978-xl4u7n6r	81	36	's	's	POS
cord-276978-xl4u7n6r	81	37	instructions	instruction	NNS
cord-276978-xl4u7n6r	81	38	,	,	,
cord-276978-xl4u7n6r	81	39	with	with	IN
cord-276978-xl4u7n6r	81	40	a	a	DT
cord-276978-xl4u7n6r	81	41	final	final	JJ
cord-276978-xl4u7n6r	81	42	elution	elution	NN
cord-276978-xl4u7n6r	81	43	volume	volume	NN
cord-276978-xl4u7n6r	81	44	of	of	IN
cord-276978-xl4u7n6r	81	45	30	30	CD
cord-276978-xl4u7n6r	81	46	l	l	NN
cord-276978-xl4u7n6r	81	47	(	(	-LRB-
cord-276978-xl4u7n6r	81	48	see	see	VB
cord-276978-xl4u7n6r	81	49	Note	Note	NNP
cord-276978-xl4u7n6r	81	50	11	11	CD
cord-276978-xl4u7n6r	81	51	)	)	-RRB-
cord-276978-xl4u7n6r	81	52	.	.	.
cord-276978-xl4u7n6r	82	1	Cycle	cycle	NN
cord-276978-xl4u7n6r	82	2	sequencing	sequencing	NN
cord-276978-xl4u7n6r	82	3	is	be	VBZ
cord-276978-xl4u7n6r	82	4	performed	perform	VBN
cord-276978-xl4u7n6r	82	5	using	use	VBG
cord-276978-xl4u7n6r	82	6	BigDye	BigDye	NNP
cord-276978-xl4u7n6r	82	7	Terminator	Terminator	NNP
cord-276978-xl4u7n6r	82	8	version	version	NN
cord-276978-xl4u7n6r	82	9	3.1	3.1	CD
cord-276978-xl4u7n6r	82	10	,	,	,
cord-276978-xl4u7n6r	82	11	for	for	IN
cord-276978-xl4u7n6r	82	12	long	long	JJ
cord-276978-xl4u7n6r	82	13	PCR	PCR	NNP
cord-276978-xl4u7n6r	82	14	product	product	NN
cord-276978-xl4u7n6r	82	15	sequencing	sequencing	NN
cord-276978-xl4u7n6r	82	16	,	,	,
cord-276978-xl4u7n6r	82	17	with	with	IN
cord-276978-xl4u7n6r	82	18	the	the	DT
cord-276978-xl4u7n6r	82	19	following	follow	VBG
cord-276978-xl4u7n6r	82	20	reagents	reagent	NNS
cord-276978-xl4u7n6r	82	21	:	:	:
cord-276978-xl4u7n6r	82	22	4	4	CD
cord-276978-xl4u7n6r	82	23	l	l	NNP
cord-276978-xl4u7n6r	82	24	BigDye	BigDye	NNP
cord-276978-xl4u7n6r	82	25	Terminator	Terminator	NNP
cord-276978-xl4u7n6r	82	26	mix	mix	NN
cord-276978-xl4u7n6r	82	27	,	,	,
cord-276978-xl4u7n6r	82	28	1.3	1.3	CD
cord-276978-xl4u7n6r	82	29	l	l	NN
cord-276978-xl4u7n6r	82	30	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	82	31	-	-	HYPH
cord-276978-xl4u7n6r	82	32	specific	specific	JJ
cord-276978-xl4u7n6r	82	33	reverse	reverse	NN
cord-276978-xl4u7n6r	82	34	primer	primer	NN
cord-276978-xl4u7n6r	82	35	2	2	CD
cord-276978-xl4u7n6r	82	36	(	(	-LRB-
cord-276978-xl4u7n6r	82	37	2.5	2.5	CD
cord-276978-xl4u7n6r	82	38	M	M	NNP
cord-276978-xl4u7n6r	82	39	)	)	-RRB-
cord-276978-xl4u7n6r	82	40	,	,	,
cord-276978-xl4u7n6r	82	41	and	and	CC
cord-276978-xl4u7n6r	82	42	4.7	4.7	CD
cord-276978-xl4u7n6r	82	43	l	l	NN
cord-276978-xl4u7n6r	82	44	PCR	PCR	NNP
cord-276978-xl4u7n6r	82	45	product	product	NN
cord-276978-xl4u7n6r	82	46	.	.	.
cord-276978-xl4u7n6r	83	1	The	the	DT
cord-276978-xl4u7n6r	83	2	cycle	cycle	NN
cord-276978-xl4u7n6r	83	3	sequencing	sequencing	NN
cord-276978-xl4u7n6r	83	4	program	program	NN
cord-276978-xl4u7n6r	83	5	consists	consist	VBZ
cord-276978-xl4u7n6r	83	6	of	of	IN
cord-276978-xl4u7n6r	83	7	an	an	DT
cord-276978-xl4u7n6r	83	8	initial	initial	JJ
cord-276978-xl4u7n6r	83	9	step	step	NN
cord-276978-xl4u7n6r	83	10	at	at	IN
cord-276978-xl4u7n6r	83	11	96	96	CD
cord-276978-xl4u7n6r	84	1	•	•	NNP
cord-276978-xl4u7n6r	85	1	C	C	NNP
cord-276978-xl4u7n6r	85	2	for	for	IN
cord-276978-xl4u7n6r	85	3	1	1	CD
cord-276978-xl4u7n6r	85	4	min	min	NN
cord-276978-xl4u7n6r	85	5	,	,	,
cord-276978-xl4u7n6r	85	6	followed	follow	VBN
cord-276978-xl4u7n6r	85	7	by	by	IN
cord-276978-xl4u7n6r	85	8	25	25	CD
cord-276978-xl4u7n6r	85	9	cycles	cycle	NNS
cord-276978-xl4u7n6r	85	10	of	of	IN
cord-276978-xl4u7n6r	85	11	96	96	CD
cord-276978-xl4u7n6r	85	12	•	•	NN
cord-276978-xl4u7n6r	85	13	C	C	NNP
cord-276978-xl4u7n6r	85	14	for	for	IN
cord-276978-xl4u7n6r	85	15	10	10	CD
cord-276978-xl4u7n6r	85	16	sec	sec	NNS
cord-276978-xl4u7n6r	85	17	,	,	,
cord-276978-xl4u7n6r	86	1	50	50	CD
cord-276978-xl4u7n6r	86	2	•	•	NN
cord-276978-xl4u7n6r	86	3	C	c	NN
cord-276978-xl4u7n6r	86	4	for	for	IN
cord-276978-xl4u7n6r	86	5	5	5	CD
cord-276978-xl4u7n6r	86	6	sec	sec	NNP
cord-276978-xl4u7n6r	86	7	,	,	,
cord-276978-xl4u7n6r	86	8	and	and	CC
cord-276978-xl4u7n6r	86	9	60	60	CD
cord-276978-xl4u7n6r	87	1	•	•	NNP
cord-276978-xl4u7n6r	88	1	C	C	NNP
cord-276978-xl4u7n6r	88	2	for	for	IN
cord-276978-xl4u7n6r	88	3	4	4	CD
cord-276978-xl4u7n6r	88	4	min	min	NN
cord-276978-xl4u7n6r	88	5	,	,	,
cord-276978-xl4u7n6r	88	6	followed	follow	VBN
cord-276978-xl4u7n6r	88	7	by	by	IN
cord-276978-xl4u7n6r	88	8	cooling	cool	VBG
cord-276978-xl4u7n6r	88	9	at	at	IN
cord-276978-xl4u7n6r	88	10	4	4	CD
cord-276978-xl4u7n6r	88	11	•	•	NNP
cord-276978-xl4u7n6r	88	12	C.	C.	NNP
cord-276978-xl4u7n6r	88	13	7	7	CD
cord-276978-xl4u7n6r	88	14	.	.	.
cord-276978-xl4u7n6r	89	1	The	the	DT
cord-276978-xl4u7n6r	89	2	cycle	cycle	NN
cord-276978-xl4u7n6r	89	3	sequencing	sequencing	NN
cord-276978-xl4u7n6r	89	4	products	product	NNS
cord-276978-xl4u7n6r	89	5	are	be	VBP
cord-276978-xl4u7n6r	89	6	purified	purify	VBN
cord-276978-xl4u7n6r	89	7	from	from	IN
cord-276978-xl4u7n6r	89	8	unincorporated	unincorporated	JJ
cord-276978-xl4u7n6r	89	9	labeled	label	VBN
cord-276978-xl4u7n6r	89	10	dideoxynucleotides	dideoxynucleotide	NNS
cord-276978-xl4u7n6r	89	11	and	and	CC
cord-276978-xl4u7n6r	89	12	separated	separate	VBN
cord-276978-xl4u7n6r	89	13	by	by	IN
cord-276978-xl4u7n6r	89	14	capillary	capillary	JJ
cord-276978-xl4u7n6r	89	15	electrophoresis	electrophoresis	NN
cord-276978-xl4u7n6r	89	16	on	on	IN
cord-276978-xl4u7n6r	89	17	a	a	DT
cord-276978-xl4u7n6r	89	18	3130xl	3130xl	JJ
cord-276978-xl4u7n6r	89	19	Genetic	Genetic	NNP
cord-276978-xl4u7n6r	89	20	Analyser	analyser	NN
cord-276978-xl4u7n6r	89	21	for	for	IN
cord-276978-xl4u7n6r	89	22	sequence	sequence	NN
cord-276978-xl4u7n6r	89	23	determination	determination	NN
cord-276978-xl4u7n6r	89	24	,	,	,
cord-276978-xl4u7n6r	89	25	according	accord	VBG
cord-276978-xl4u7n6r	89	26	to	to	IN
cord-276978-xl4u7n6r	89	27	Applied	Applied	NNP
cord-276978-xl4u7n6r	89	28	Biosystems	Biosystems	NNP
cord-276978-xl4u7n6r	89	29	'	'	POS
cord-276978-xl4u7n6r	89	30	instructions	instruction	NNS
cord-276978-xl4u7n6r	89	31	(	(	-LRB-
cord-276978-xl4u7n6r	89	32	see	see	VB
cord-276978-xl4u7n6r	89	33	Note	Note	NNP
cord-276978-xl4u7n6r	89	34	12	12	CD
cord-276978-xl4u7n6r	89	35	)	)	-RRB-
cord-276978-xl4u7n6r	89	36	.	.	.
cord-276978-xl4u7n6r	90	1	1	1	LS
cord-276978-xl4u7n6r	90	2	.	.	.
cord-276978-xl4u7n6r	91	1	In	in	IN
cord-276978-xl4u7n6r	91	2	addition	addition	NN
cord-276978-xl4u7n6r	91	3	to	to	IN
cord-276978-xl4u7n6r	91	4	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	91	5	,	,	,
cord-276978-xl4u7n6r	91	6	this	this	DT
cord-276978-xl4u7n6r	91	7	procedure	procedure	NN
cord-276978-xl4u7n6r	91	8	has	have	VBZ
cord-276978-xl4u7n6r	91	9	been	be	VBN
cord-276978-xl4u7n6r	91	10	successfully	successfully	RB
cord-276978-xl4u7n6r	91	11	applied	apply	VBN
cord-276978-xl4u7n6r	91	12	to	to	TO
cord-276978-xl4u7n6r	91	13	amplify	amplify	VB
cord-276978-xl4u7n6r	91	14	and	and	CC
cord-276978-xl4u7n6r	91	15	sequence	sequence	VB
cord-276978-xl4u7n6r	91	16	novel	novel	NN
cord-276978-xl4u7n6r	91	17	astrovirus	astrovirus	NNP
cord-276978-xl4u7n6r	91	18	and	and	CC
cord-276978-xl4u7n6r	91	19	picornavirus	picornavirus	NNP
cord-276978-xl4u7n6r	91	20	genomes	genome	NNS
cord-276978-xl4u7n6r	91	21	,	,	,
cord-276978-xl4u7n6r	91	22	and	and	CC
cord-276978-xl4u7n6r	91	23	potentially	potentially	RB
cord-276978-xl4u7n6r	91	24	all	all	DT
cord-276978-xl4u7n6r	91	25	polyadenylated	polyadenylate	VBN
cord-276978-xl4u7n6r	91	26	viral	viral	JJ
cord-276978-xl4u7n6r	91	27	genomes	genome	NNS
cord-276978-xl4u7n6r	91	28	harboring	harbor	VBG
cord-276978-xl4u7n6r	91	29	s2	s2	NNP
cord-276978-xl4u7n6r	91	30	m	m	NNP
cord-276978-xl4u7n6r	91	31	can	can	MD
cord-276978-xl4u7n6r	91	32	be	be	VB
cord-276978-xl4u7n6r	91	33	identified	identify	VBN
cord-276978-xl4u7n6r	91	34	and	and	CC
cord-276978-xl4u7n6r	91	35	characterized	characterize	VBN
cord-276978-xl4u7n6r	91	36	with	with	IN
cord-276978-xl4u7n6r	91	37	this	this	DT
cord-276978-xl4u7n6r	91	38	procedure	procedure	NN
cord-276978-xl4u7n6r	91	39	.	.	.
cord-276978-xl4u7n6r	92	1	However	however	RB
cord-276978-xl4u7n6r	92	2	,	,	,
cord-276978-xl4u7n6r	92	3	when	when	WRB
cord-276978-xl4u7n6r	92	4	used	use	VBN
cord-276978-xl4u7n6r	92	5	on	on	IN
cord-276978-xl4u7n6r	92	6	biological	biological	JJ
cord-276978-xl4u7n6r	92	7	samples	sample	NNS
cord-276978-xl4u7n6r	92	8	that	that	WDT
cord-276978-xl4u7n6r	92	9	do	do	VBP
cord-276978-xl4u7n6r	92	10	not	not	RB
cord-276978-xl4u7n6r	92	11	contain	contain	VB
cord-276978-xl4u7n6r	92	12	s2m	s2m	NNP
cord-276978-xl4u7n6r	92	13	-	-	HYPH
cord-276978-xl4u7n6r	92	14	harboring	harbor	VBG
cord-276978-xl4u7n6r	92	15	viruses	virus	NNS
cord-276978-xl4u7n6r	92	16	,	,	,
cord-276978-xl4u7n6r	92	17	nonspecific	nonspecific	JJ
cord-276978-xl4u7n6r	92	18	amplification	amplification	NN
cord-276978-xl4u7n6r	92	19	of	of	IN
cord-276978-xl4u7n6r	92	20	host	host	NN
cord-276978-xl4u7n6r	92	21	cell	cell	NN
cord-276978-xl4u7n6r	92	22	ribosomal	ribosomal	NNP
cord-276978-xl4u7n6r	92	23	RNA	RNA	NNP
cord-276978-xl4u7n6r	92	24	is	be	VBZ
cord-276978-xl4u7n6r	92	25	obtained	obtain	VBN
cord-276978-xl4u7n6r	92	26	on	on	IN
cord-276978-xl4u7n6r	92	27	some	some	DT
cord-276978-xl4u7n6r	92	28	occasions	occasion	NNS
cord-276978-xl4u7n6r	92	29	.	.	.
cord-276978-xl4u7n6r	93	1	2	2	LS
cord-276978-xl4u7n6r	93	2	.	.	.
cord-276978-xl4u7n6r	94	1	We	-PRON-	PRP
cord-276978-xl4u7n6r	94	2	have	have	VBP
cord-276978-xl4u7n6r	94	3	generally	generally	RB
cord-276978-xl4u7n6r	94	4	used	use	VBN
cord-276978-xl4u7n6r	94	5	1	1	CD
cord-276978-xl4u7n6r	94	6	l	l	NN
cord-276978-xl4u7n6r	94	7	anchored	anchor	VBD
cord-276978-xl4u7n6r	94	8	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	94	9	)	)	-RRB-
cord-276978-xl4u7n6r	94	10	18	18	CD
cord-276978-xl4u7n6r	94	11	(	(	-LRB-
cord-276978-xl4u7n6r	94	12	25	25	CD
cord-276978-xl4u7n6r	94	13	M	M	NNP
cord-276978-xl4u7n6r	94	14	)	)	-RRB-
cord-276978-xl4u7n6r	94	15	in	in	IN
cord-276978-xl4u7n6r	94	16	the	the	DT
cord-276978-xl4u7n6r	94	17	RT	RT	NNP
cord-276978-xl4u7n6r	94	18	,	,	,
cord-276978-xl4u7n6r	94	19	but	but	CC
cord-276978-xl4u7n6r	94	20	1	1	CD
cord-276978-xl4u7n6r	94	21	l	l	NN
cord-276978-xl4u7n6r	94	22	Invitrogens	Invitrogens	NNP
cord-276978-xl4u7n6r	94	23	anchored	anchor	VBD
cord-276978-xl4u7n6r	94	24	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	94	25	)	)	-RRB-
cord-276978-xl4u7n6r	94	26	20	20	CD
cord-276978-xl4u7n6r	94	27	(	(	-LRB-
cord-276978-xl4u7n6r	94	28	2.5	2.5	CD
cord-276978-xl4u7n6r	94	29	g	g	NN
cord-276978-xl4u7n6r	94	30	/	/	SYM
cord-276978-xl4u7n6r	94	31	l	l	NN
cord-276978-xl4u7n6r	94	32	,	,	,
cord-276978-xl4u7n6r	94	33	corresponding	correspond	VBG
cord-276978-xl4u7n6r	94	34	to	to	IN
cord-276978-xl4u7n6r	94	35	ca	ca	NNP
cord-276978-xl4u7n6r	94	36	.	.	.
cord-276978-xl4u7n6r	95	1	350	350	CD
cord-276978-xl4u7n6r	95	2	M	M	NNP
cord-276978-xl4u7n6r	95	3	)	)	-RRB-
cord-276978-xl4u7n6r	95	4	,	,	,
cord-276978-xl4u7n6r	95	5	or	or	CC
cord-276978-xl4u7n6r	95	6	Invitrogens	Invitrogens	NNP
cord-276978-xl4u7n6r	95	7	standard	standard	NN
cord-276978-xl4u7n6r	95	8	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	95	9	)	)	-RRB-
cord-276978-xl4u7n6r	95	10	20	20	CD
cord-276978-xl4u7n6r	95	11	(	(	-LRB-
cord-276978-xl4u7n6r	95	12	50	50	CD
cord-276978-xl4u7n6r	95	13	M	M	NNP
cord-276978-xl4u7n6r	95	14	)	)	-RRB-
cord-276978-xl4u7n6r	95	15	,	,	,
cord-276978-xl4u7n6r	95	16	has	have	VBZ
cord-276978-xl4u7n6r	95	17	also	also	RB
cord-276978-xl4u7n6r	95	18	been	be	VBN
cord-276978-xl4u7n6r	95	19	used	use	VBN
cord-276978-xl4u7n6r	95	20	successfully	successfully	RB
cord-276978-xl4u7n6r	95	21	as	as	IN
cord-276978-xl4u7n6r	95	22	a	a	DT
cord-276978-xl4u7n6r	95	23	reverse	reverse	JJ
cord-276978-xl4u7n6r	95	24	primer	primer	NN
cord-276978-xl4u7n6r	95	25	.	.	.
cord-276978-xl4u7n6r	96	1	3	3	LS
cord-276978-xl4u7n6r	96	2	.	.	.
cord-276978-xl4u7n6r	97	1	The	the	DT
cord-276978-xl4u7n6r	97	2	obtained	obtain	VBN
cord-276978-xl4u7n6r	97	3	PCR	pcr	NN
cord-276978-xl4u7n6r	97	4	products	product	NNS
cord-276978-xl4u7n6r	97	5	often	often	RB
cord-276978-xl4u7n6r	97	6	give	give	VBP
cord-276978-xl4u7n6r	97	7	a	a	DT
cord-276978-xl4u7n6r	97	8	smear	smear	NN
cord-276978-xl4u7n6r	97	9	on	on	IN
cord-276978-xl4u7n6r	97	10	visualization	visualization	NN
cord-276978-xl4u7n6r	97	11	on	on	IN
cord-276978-xl4u7n6r	97	12	agarose	agarose	JJ
cord-276978-xl4u7n6r	97	13	gel	gel	NN
cord-276978-xl4u7n6r	97	14	,	,	,
cord-276978-xl4u7n6r	97	15	owing	owe	VBG
cord-276978-xl4u7n6r	97	16	to	to	IN
cord-276978-xl4u7n6r	97	17	serial	serial	JJ
cord-276978-xl4u7n6r	97	18	mispriming	mispriming	NN
cord-276978-xl4u7n6r	97	19	of	of	IN
cord-276978-xl4u7n6r	97	20	the	the	DT
cord-276978-xl4u7n6r	97	21	anchored	anchored	JJ
cord-276978-xl4u7n6r	97	22	oligo(dT	oligo(dt	CD
cord-276978-xl4u7n6r	97	23	)	)	-RRB-
cord-276978-xl4u7n6r	97	24	primer	primer	NN
cord-276978-xl4u7n6r	97	25	on	on	IN
cord-276978-xl4u7n6r	97	26	the	the	DT
cord-276978-xl4u7n6r	97	27	3	3	CD
cord-276978-xl4u7n6r	97	28	poly(A	poly(a	JJ
cord-276978-xl4u7n6r	97	29	)	)	-RRB-
cord-276978-xl4u7n6r	97	30	tail	tail	NN
cord-276978-xl4u7n6r	97	31	,	,	,
cord-276978-xl4u7n6r	97	32	leading	lead	VBG
cord-276978-xl4u7n6r	97	33	to	to	IN
cord-276978-xl4u7n6r	97	34	long	long	JJ
cord-276978-xl4u7n6r	97	35	-	-	HYPH
cord-276978-xl4u7n6r	97	36	range	range	NN
cord-276978-xl4u7n6r	97	37	accumulation	accumulation	NN
cord-276978-xl4u7n6r	97	38	of	of	IN
cord-276978-xl4u7n6r	97	39	As	as	IN
cord-276978-xl4u7n6r	97	40	in	in	IN
cord-276978-xl4u7n6r	97	41	the	the	DT
cord-276978-xl4u7n6r	97	42	PCR	PCR	NNP
cord-276978-xl4u7n6r	97	43	products	product	NNS
cord-276978-xl4u7n6r	97	44	that	that	WDT
cord-276978-xl4u7n6r	97	45	can	can	MD
cord-276978-xl4u7n6r	97	46	be	be	VB
cord-276978-xl4u7n6r	97	47	observed	observe	VBN
cord-276978-xl4u7n6r	97	48	upon	upon	IN
cord-276978-xl4u7n6r	97	49	sequencing	sequence	VBG
cord-276978-xl4u7n6r	97	50	.	.	.
cord-276978-xl4u7n6r	98	1	However	however	RB
cord-276978-xl4u7n6r	98	2	,	,	,
cord-276978-xl4u7n6r	98	3	if	if	IN
cord-276978-xl4u7n6r	98	4	amplification	amplification	NN
cord-276978-xl4u7n6r	98	5	is	be	VBZ
cord-276978-xl4u7n6r	98	6	successful	successful	JJ
cord-276978-xl4u7n6r	98	7	,	,	,
cord-276978-xl4u7n6r	98	8	all	all	DT
cord-276978-xl4u7n6r	98	9	PCR	PCR	NNP
cord-276978-xl4u7n6r	98	10	products	product	NNS
cord-276978-xl4u7n6r	98	11	,	,	,
cord-276978-xl4u7n6r	98	12	regardless	regardless	RB
cord-276978-xl4u7n6r	98	13	of	of	IN
cord-276978-xl4u7n6r	98	14	the	the	DT
cord-276978-xl4u7n6r	98	15	number	number	NN
cord-276978-xl4u7n6r	98	16	of	of	IN
cord-276978-xl4u7n6r	98	17	terminal	terminal	NN
cord-276978-xl4u7n6r	99	1	As	as	IN
cord-276978-xl4u7n6r	99	2	,	,	,
cord-276978-xl4u7n6r	99	3	have	have	VBP
cord-276978-xl4u7n6r	99	4	the	the	DT
cord-276978-xl4u7n6r	99	5	same	same	JJ
cord-276978-xl4u7n6r	99	6	start	start	NN
cord-276978-xl4u7n6r	99	7	at	at	IN
cord-276978-xl4u7n6r	99	8	s2	s2	NNP
cord-276978-xl4u7n6r	99	9	m	m	NNP
cord-276978-xl4u7n6r	99	10	,	,	,
cord-276978-xl4u7n6r	99	11	as	as	IN
cord-276978-xl4u7n6r	99	12	the	the	DT
cord-276978-xl4u7n6r	99	13	s2m	s2m	JJ
cord-276978-xl4u7n6r	99	14	-	-	HYPH
cord-276978-xl4u7n6r	99	15	core	core	JJ
cord-276978-xl4u7n6r	99	16	primer	primer	NN
cord-276978-xl4u7n6r	99	17	is	be	VBZ
cord-276978-xl4u7n6r	99	18	highly	highly	RB
cord-276978-xl4u7n6r	99	19	specific	specific	JJ
cord-276978-xl4u7n6r	99	20	,	,	,
cord-276978-xl4u7n6r	99	21	and	and	CC
cord-276978-xl4u7n6r	99	22	the	the	DT
cord-276978-xl4u7n6r	99	23	PCR	PCR	NNP
cord-276978-xl4u7n6r	99	24	product	product	NN
cord-276978-xl4u7n6r	99	25	smear	smear	NN
cord-276978-xl4u7n6r	99	26	can	can	MD
cord-276978-xl4u7n6r	99	27	be	be	VB
cord-276978-xl4u7n6r	99	28	sequenced	sequence	VBN
cord-276978-xl4u7n6r	99	29	using	use	VBG
cord-276978-xl4u7n6r	99	30	s2m	s2m	JJ
cord-276978-xl4u7n6r	99	31	-	-	HYPH
cord-276978-xl4u7n6r	99	32	core	core	JJ
cord-276978-xl4u7n6r	99	33	or	or	CC
cord-276978-xl4u7n6r	99	34	s2m	s2m	JJ
cord-276978-xl4u7n6r	99	35	-	-	HYPH
cord-276978-xl4u7n6r	99	36	coreseq	coreseq	JJ
cord-276978-xl4u7n6r	99	37	primer	primer	NN
cord-276978-xl4u7n6r	99	38	.	.	.
cord-276978-xl4u7n6r	100	1	4	4	LS
cord-276978-xl4u7n6r	100	2	.	.	.
cord-276978-xl4u7n6r	101	1	As	as	IN
cord-276978-xl4u7n6r	101	2	several	several	JJ
cord-276978-xl4u7n6r	101	3	of	of	IN
cord-276978-xl4u7n6r	101	4	the	the	DT
cord-276978-xl4u7n6r	101	5	viruses	virus	NNS
cord-276978-xl4u7n6r	101	6	harboring	harbor	VBG
cord-276978-xl4u7n6r	101	7	s2	s2	NNP
cord-276978-xl4u7n6r	101	8	m	m	NNP
cord-276978-xl4u7n6r	101	9	do	do	TO
cord-276978-xl4u7n6r	101	10	terminate	terminate	VB
cord-276978-xl4u7n6r	101	11	the	the	DT
cord-276978-xl4u7n6r	101	12	genome	genome	NN
cord-276978-xl4u7n6r	101	13	with	with	IN
cord-276978-xl4u7n6r	101	14	GC(A)n	GC(A)n	NNP
cord-276978-xl4u7n6r	101	15	,	,	,
cord-276978-xl4u7n6r	101	16	AV12	AV12	NNP
cord-276978-xl4u7n6r	101	17	(	(	-LRB-
cord-276978-xl4u7n6r	101	18	2.5	2.5	CD
cord-276978-xl4u7n6r	101	19	M	M	NNP
cord-276978-xl4u7n6r	101	20	)	)	-RRB-
cord-276978-xl4u7n6r	101	21	can	can	MD
cord-276978-xl4u7n6r	101	22	often	often	RB
cord-276978-xl4u7n6r	101	23	be	be	VB
cord-276978-xl4u7n6r	101	24	used	use	VBN
cord-276978-xl4u7n6r	101	25	for	for	IN
cord-276978-xl4u7n6r	101	26	sequencing	sequence	VBG
cord-276978-xl4u7n6r	101	27	the	the	DT
cord-276978-xl4u7n6r	101	28	opposite	opposite	JJ
cord-276978-xl4u7n6r	101	29	strand	strand	NN
cord-276978-xl4u7n6r	101	30	.	.	.
cord-276978-xl4u7n6r	102	1	If	if	IN
cord-276978-xl4u7n6r	102	2	low	low	JJ
cord-276978-xl4u7n6r	102	3	signal	signal	NN
cord-276978-xl4u7n6r	102	4	intensity	intensity	NN
cord-276978-xl4u7n6r	102	5	is	be	VBZ
cord-276978-xl4u7n6r	102	6	obtained	obtain	VBN
cord-276978-xl4u7n6r	102	7	for	for	IN
cord-276978-xl4u7n6r	102	8	the	the	DT
cord-276978-xl4u7n6r	102	9	sequences	sequence	NNS
cord-276978-xl4u7n6r	102	10	,	,	,
cord-276978-xl4u7n6r	102	11	the	the	DT
cord-276978-xl4u7n6r	102	12	following	following	JJ
cord-276978-xl4u7n6r	102	13	modifications	modification	NNS
cord-276978-xl4u7n6r	102	14	can	can	MD
cord-276978-xl4u7n6r	102	15	be	be	VB
cord-276978-xl4u7n6r	102	16	made	make	VBN
cord-276978-xl4u7n6r	102	17	in	in	IN
cord-276978-xl4u7n6r	102	18	the	the	DT
cord-276978-xl4u7n6r	102	19	cycle	cycle	NN
cord-276978-xl4u7n6r	102	20	sequencing	sequencing	NN
cord-276978-xl4u7n6r	102	21	program	program	NN
cord-276978-xl4u7n6r	102	22	:	:	:
cord-276978-xl4u7n6r	102	23	lowering	lower	VBG
cord-276978-xl4u7n6r	102	24	the	the	DT
cord-276978-xl4u7n6r	102	25	annealing	anneal	VBG
cord-276978-xl4u7n6r	102	26	temperature	temperature	NN
cord-276978-xl4u7n6r	102	27	to	to	IN
cord-276978-xl4u7n6r	102	28	50	50	CD
cord-276978-xl4u7n6r	102	29	•	•	NN
cord-276978-xl4u7n6r	102	30	C	c	NN
cord-276978-xl4u7n6r	103	1	and/or	and/or	CC
cord-276978-xl4u7n6r	103	2	adding	add	VBG
cord-276978-xl4u7n6r	103	3	5	5	CD
cord-276978-xl4u7n6r	103	4	-	-	SYM
cord-276978-xl4u7n6r	103	5	10	10	CD
cord-276978-xl4u7n6r	103	6	cycles	cycle	NNS
cord-276978-xl4u7n6r	103	7	in	in	IN
cord-276978-xl4u7n6r	103	8	the	the	DT
cord-276978-xl4u7n6r	103	9	cycle	cycle	NN
cord-276978-xl4u7n6r	103	10	sequencing	sequencing	NN
cord-276978-xl4u7n6r	103	11	reaction	reaction	NN
cord-276978-xl4u7n6r	103	12	.	.	.
cord-276978-xl4u7n6r	104	1	5	5	LS
cord-276978-xl4u7n6r	104	2	.	.	.
cord-276978-xl4u7n6r	105	1	Once	once	RB
cord-276978-xl4u7n6r	105	2	a	a	DT
cord-276978-xl4u7n6r	105	3	specific	specific	JJ
cord-276978-xl4u7n6r	105	4	reverse	reverse	NN
cord-276978-xl4u7n6r	105	5	primer	primer	NN
cord-276978-xl4u7n6r	105	6	can	can	MD
cord-276978-xl4u7n6r	105	7	be	be	VB
cord-276978-xl4u7n6r	105	8	designed	design	VBN
cord-276978-xl4u7n6r	105	9	as	as	IN
cord-276978-xl4u7n6r	105	10	described	describe	VBN
cord-276978-xl4u7n6r	105	11	in	in	IN
cord-276978-xl4u7n6r	105	12	Section	Section	NNP
cord-276978-xl4u7n6r	105	13	3.2	3.2	CD
cord-276978-xl4u7n6r	105	14	,	,	,
cord-276978-xl4u7n6r	105	15	an	an	DT
cord-276978-xl4u7n6r	105	16	alternative	alternative	JJ
cord-276978-xl4u7n6r	105	17	strategy	strategy	NN
cord-276978-xl4u7n6r	105	18	to	to	IN
cord-276978-xl4u7n6r	105	19	5	5	CD
cord-276978-xl4u7n6r	105	20	-RACE	-race	NN
cord-276978-xl4u7n6r	105	21	/	/	SYM
cord-276978-xl4u7n6r	105	22	primer	primer	NN
cord-276978-xl4u7n6r	105	23	walking	walking	NN
cord-276978-xl4u7n6r	105	24	is	be	VBZ
cord-276978-xl4u7n6r	105	25	to	to	TO
cord-276978-xl4u7n6r	105	26	amplify	amplify	VB
cord-276978-xl4u7n6r	105	27	directly	directly	RB
cord-276978-xl4u7n6r	105	28	a	a	DT
cord-276978-xl4u7n6r	105	29	long	long	JJ
cord-276978-xl4u7n6r	105	30	PCR	PCR	NNP
cord-276978-xl4u7n6r	105	31	product	product	NN
cord-276978-xl4u7n6r	105	32	using	use	VBG
cord-276978-xl4u7n6r	105	33	upstream	upstream	JJ
cord-276978-xl4u7n6r	105	34	primers	primer	NNS
cord-276978-xl4u7n6r	105	35	in	in	IN
cord-276978-xl4u7n6r	105	36	conserved	conserved	JJ
cord-276978-xl4u7n6r	105	37	family	family	NN
cord-276978-xl4u7n6r	105	38	-	-	HYPH
cord-276978-xl4u7n6r	105	39	specific	specific	JJ
cord-276978-xl4u7n6r	105	40	motifs	motif	NNS
cord-276978-xl4u7n6r	105	41	,	,	,
cord-276978-xl4u7n6r	105	42	e.g.	e.g.	RB
cord-276978-xl4u7n6r	105	43	,	,	,
cord-276978-xl4u7n6r	105	44	in	in	IN
cord-276978-xl4u7n6r	105	45	the	the	DT
cord-276978-xl4u7n6r	105	46	polymerase	polymerase	NN
cord-276978-xl4u7n6r	105	47	gene	gene	NN
cord-276978-xl4u7n6r	105	48	.	.	.
cord-276978-xl4u7n6r	106	1	This	this	DT
cord-276978-xl4u7n6r	106	2	will	will	MD
cord-276978-xl4u7n6r	106	3	be	be	VB
cord-276978-xl4u7n6r	106	4	achieved	achieve	VBN
cord-276978-xl4u7n6r	106	5	more	more	RBR
cord-276978-xl4u7n6r	106	6	easily	easily	RB
cord-276978-xl4u7n6r	106	7	with	with	IN
cord-276978-xl4u7n6r	106	8	viruses	virus	NNS
cord-276978-xl4u7n6r	106	9	that	that	WDT
cord-276978-xl4u7n6r	106	10	have	have	VBP
cord-276978-xl4u7n6r	106	11	small	small	JJ
cord-276978-xl4u7n6r	106	12	genomes	genome	NNS
cord-276978-xl4u7n6r	106	13	,	,	,
cord-276978-xl4u7n6r	106	14	such	such	JJ
cord-276978-xl4u7n6r	106	15	as	as	IN
cord-276978-xl4u7n6r	106	16	astrovirus	astrovirus	NNP
cord-276978-xl4u7n6r	106	17	and	and	CC
cord-276978-xl4u7n6r	106	18	picornavirus	picornavirus	NNP
cord-276978-xl4u7n6r	106	19	,	,	,
cord-276978-xl4u7n6r	106	20	especially	especially	RB
cord-276978-xl4u7n6r	106	21	when	when	WRB
cord-276978-xl4u7n6r	106	22	the	the	DT
cord-276978-xl4u7n6r	106	23	polymerase	polymerase	NN
cord-276978-xl4u7n6r	106	24	is	be	VBZ
cord-276978-xl4u7n6r	106	25	encoded	encode	VBN
cord-276978-xl4u7n6r	106	26	toward	toward	IN
cord-276978-xl4u7n6r	106	27	the	the	DT
cord-276978-xl4u7n6r	106	28	3	3	CD
cord-276978-xl4u7n6r	106	29	-end	-end	:
cord-276978-xl4u7n6r	106	30	of	of	IN
cord-276978-xl4u7n6r	106	31	the	the	DT
cord-276978-xl4u7n6r	106	32	genome	genome	NN
cord-276978-xl4u7n6r	106	33	,	,	,
cord-276978-xl4u7n6r	106	34	rather	rather	RB
cord-276978-xl4u7n6r	106	35	than	than	IN
cord-276978-xl4u7n6r	106	36	with	with	IN
cord-276978-xl4u7n6r	106	37	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	106	38	that	that	WDT
cord-276978-xl4u7n6r	106	39	have	have	VBP
cord-276978-xl4u7n6r	106	40	a	a	DT
cord-276978-xl4u7n6r	106	41	large	large	JJ
cord-276978-xl4u7n6r	106	42	genome	genome	NN
cord-276978-xl4u7n6r	106	43	.	.	.
cord-276978-xl4u7n6r	107	1	In	in	IN
cord-276978-xl4u7n6r	107	2	our	-PRON-	PRP$
cord-276978-xl4u7n6r	107	3	laboratory	laboratory	NN
cord-276978-xl4u7n6r	107	4	,	,	,
cord-276978-xl4u7n6r	107	5	we	-PRON-	PRP
cord-276978-xl4u7n6r	107	6	were	be	VBD
cord-276978-xl4u7n6r	107	7	able	able	JJ
cord-276978-xl4u7n6r	107	8	to	to	TO
cord-276978-xl4u7n6r	107	9	get	get	VB
cord-276978-xl4u7n6r	107	10	sequence	sequence	NN
cord-276978-xl4u7n6r	107	11	information	information	NN
cord-276978-xl4u7n6r	107	12	by	by	IN
cord-276978-xl4u7n6r	107	13	using	use	VBG
cord-276978-xl4u7n6r	107	14	the	the	DT
cord-276978-xl4u7n6r	107	15	sense	sense	NN
cord-276978-xl4u7n6r	108	1	2Bm	2bm	JJ
cord-276978-xl4u7n6r	108	2	primer	primer	NN
cord-276978-xl4u7n6r	108	3	designed	design	VBN
cord-276978-xl4u7n6r	108	4	by	by	IN
cord-276978-xl4u7n6r	108	5	Stephensen	Stephensen	NNP
cord-276978-xl4u7n6r	108	6	et	et	NNP
cord-276978-xl4u7n6r	108	7	al	al	NNP
cord-276978-xl4u7n6r	108	8	.	.	.
cord-276978-xl4u7n6r	109	1	in	in	IN
cord-276978-xl4u7n6r	109	2	1999	1999	CD
cord-276978-xl4u7n6r	109	3	(	(	-LRB-
cord-276978-xl4u7n6r	109	4	7	7	CD
cord-276978-xl4u7n6r	109	5	)	)	-RRB-
cord-276978-xl4u7n6r	109	6	toward	toward	IN
cord-276978-xl4u7n6r	109	7	the	the	DT
cord-276978-xl4u7n6r	109	8	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	109	9	specific	specific	JJ
cord-276978-xl4u7n6r	109	10	primer	primer	NN
cord-276978-xl4u7n6r	109	11	at	at	IN
cord-276978-xl4u7n6r	109	12	the	the	DT
cord-276978-xl4u7n6r	109	13	3	3	CD
cord-276978-xl4u7n6r	109	14	-end	-end	:
cord-276978-xl4u7n6r	109	15	of	of	IN
cord-276978-xl4u7n6r	109	16	the	the	DT
cord-276978-xl4u7n6r	109	17	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	109	18	genome	genome	NN
cord-276978-xl4u7n6r	109	19	.	.	.
cord-276978-xl4u7n6r	110	1	However	however	RB
cord-276978-xl4u7n6r	110	2	,	,	,
cord-276978-xl4u7n6r	110	3	the	the	DT
cord-276978-xl4u7n6r	110	4	PCR	PCR	NNP
cord-276978-xl4u7n6r	110	5	product	product	NN
cord-276978-xl4u7n6r	110	6	yield	yield	NN
cord-276978-xl4u7n6r	110	7	was	be	VBD
cord-276978-xl4u7n6r	110	8	low	low	JJ
cord-276978-xl4u7n6r	110	9	,	,	,
cord-276978-xl4u7n6r	110	10	and	and	CC
cord-276978-xl4u7n6r	110	11	only	only	RB
cord-276978-xl4u7n6r	110	12	short	short	JJ
cord-276978-xl4u7n6r	110	13	sequence	sequence	NN
cord-276978-xl4u7n6r	110	14	stretches	stretch	NNS
cord-276978-xl4u7n6r	110	15	could	could	MD
cord-276978-xl4u7n6r	110	16	be	be	VB
cord-276978-xl4u7n6r	110	17	obtained	obtain	VBN
cord-276978-xl4u7n6r	110	18	in	in	IN
cord-276978-xl4u7n6r	110	19	this	this	DT
cord-276978-xl4u7n6r	110	20	way	way	NN
cord-276978-xl4u7n6r	110	21	.	.	.
cord-276978-xl4u7n6r	111	1	6	6	LS
cord-276978-xl4u7n6r	111	2	.	.	.
cord-276978-xl4u7n6r	112	1	The	the	DT
cord-276978-xl4u7n6r	112	2	RT	RT	NNP
cord-276978-xl4u7n6r	112	3	in	in	IN
cord-276978-xl4u7n6r	112	4	the	the	DT
cord-276978-xl4u7n6r	112	5	5	5	CD
cord-276978-xl4u7n6r	112	6	-RACE	-race	NN
cord-276978-xl4u7n6r	112	7	procedure	procedure	NN
cord-276978-xl4u7n6r	112	8	is	be	VBZ
cord-276978-xl4u7n6r	112	9	laborious	laborious	JJ
cord-276978-xl4u7n6r	112	10	,	,	,
cord-276978-xl4u7n6r	112	11	as	as	IN
cord-276978-xl4u7n6r	112	12	no	no	DT
cord-276978-xl4u7n6r	112	13	mix	mix	NN
cord-276978-xl4u7n6r	112	14	containing	contain	VBG
cord-276978-xl4u7n6r	112	15	all	all	PDT
cord-276978-xl4u7n6r	112	16	the	the	DT
cord-276978-xl4u7n6r	112	17	reagents	reagent	NNS
cord-276978-xl4u7n6r	112	18	is	be	VBZ
cord-276978-xl4u7n6r	112	19	prepared	prepare	VBN
cord-276978-xl4u7n6r	112	20	in	in	IN
cord-276978-xl4u7n6r	112	21	advance	advance	NN
cord-276978-xl4u7n6r	112	22	,	,	,
cord-276978-xl4u7n6r	112	23	but	but	CC
cord-276978-xl4u7n6r	112	24	,	,	,
cord-276978-xl4u7n6r	112	25	rather	rather	RB
cord-276978-xl4u7n6r	112	26	,	,	,
cord-276978-xl4u7n6r	112	27	primer	primer	NN
cord-276978-xl4u7n6r	112	28	and	and	CC
cord-276978-xl4u7n6r	112	29	enzymes	enzyme	NNS
cord-276978-xl4u7n6r	112	30	are	be	VBP
cord-276978-xl4u7n6r	112	31	added	add	VBN
cord-276978-xl4u7n6r	112	32	individually	individually	RB
cord-276978-xl4u7n6r	112	33	to	to	IN
cord-276978-xl4u7n6r	112	34	each	each	DT
cord-276978-xl4u7n6r	112	35	sample	sample	NN
cord-276978-xl4u7n6r	112	36	.	.	.
cord-276978-xl4u7n6r	113	1	In	in	IN
cord-276978-xl4u7n6r	113	2	addition	addition	NN
cord-276978-xl4u7n6r	113	3	,	,	,
cord-276978-xl4u7n6r	113	4	the	the	DT
cord-276978-xl4u7n6r	113	5	5	5	CD
cord-276978-xl4u7n6r	113	6	-RACE	-race	NN
cord-276978-xl4u7n6r	113	7	procedure	procedure	NN
cord-276978-xl4u7n6r	113	8	described	describe	VBN
cord-276978-xl4u7n6r	113	9	in	in	IN
cord-276978-xl4u7n6r	113	10	the	the	DT
cord-276978-xl4u7n6r	113	11	Invitrogen	Invitrogen	NNP
cord-276978-xl4u7n6r	113	12	handbook	handbook	NN
cord-276978-xl4u7n6r	113	13	suggests	suggest	VBZ
cord-276978-xl4u7n6r	113	14	that	that	IN
cord-276978-xl4u7n6r	113	15	the	the	DT
cord-276978-xl4u7n6r	113	16	RNase	rnase	NN
cord-276978-xl4u7n6r	113	17	treatment	treatment	NN
cord-276978-xl4u7n6r	113	18	of	of	IN
cord-276978-xl4u7n6r	113	19	the	the	DT
cord-276978-xl4u7n6r	113	20	sample	sample	NN
cord-276978-xl4u7n6r	113	21	be	be	VB
cord-276978-xl4u7n6r	113	22	performed	perform	VBN
cord-276978-xl4u7n6r	113	23	on	on	IN
cord-276978-xl4u7n6r	113	24	freshly	freshly	RB
cord-276978-xl4u7n6r	113	25	synthesized	synthesize	VBN
cord-276978-xl4u7n6r	113	26	cDNA	cdna	NN
cord-276978-xl4u7n6r	113	27	,	,	,
cord-276978-xl4u7n6r	113	28	that	that	WDT
cord-276978-xl4u7n6r	113	29	has	have	VBZ
cord-276978-xl4u7n6r	113	30	not	not	RB
cord-276978-xl4u7n6r	113	31	been	be	VBN
cord-276978-xl4u7n6r	113	32	frozen	freeze	VBN
cord-276978-xl4u7n6r	113	33	.	.	.
cord-276978-xl4u7n6r	114	1	This	this	DT
cord-276978-xl4u7n6r	114	2	is	be	VBZ
cord-276978-xl4u7n6r	114	3	why	why	WRB
cord-276978-xl4u7n6r	114	4	the	the	DT
cord-276978-xl4u7n6r	114	5	initial	initial	JJ
cord-276978-xl4u7n6r	114	6	RT	RT	NNP
cord-276978-xl4u7n6r	114	7	step	step	NN
cord-276978-xl4u7n6r	114	8	described	describe	VBN
cord-276978-xl4u7n6r	114	9	in	in	IN
cord-276978-xl4u7n6r	114	10	Section	Section	NNP
cord-276978-xl4u7n6r	114	11	3.1	3.1	CD
cord-276978-xl4u7n6r	114	12	,	,	,
cord-276978-xl4u7n6r	114	13	performed	perform	VBN
cord-276978-xl4u7n6r	114	14	in	in	IN
cord-276978-xl4u7n6r	114	15	order	order	NN
cord-276978-xl4u7n6r	114	16	to	to	TO
cord-276978-xl4u7n6r	114	17	check	check	VB
cord-276978-xl4u7n6r	114	18	novel	novel	JJ
cord-276978-xl4u7n6r	114	19	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	114	20	genomes	genome	NNS
cord-276978-xl4u7n6r	114	21	for	for	IN
cord-276978-xl4u7n6r	114	22	the	the	DT
cord-276978-xl4u7n6r	114	23	presence	presence	NN
cord-276978-xl4u7n6r	114	24	of	of	IN
cord-276978-xl4u7n6r	114	25	s2	s2	NNP
cord-276978-xl4u7n6r	114	26	m	m	NNP
cord-276978-xl4u7n6r	114	27	,	,	,
cord-276978-xl4u7n6r	114	28	makes	make	VBZ
cord-276978-xl4u7n6r	114	29	use	use	NN
cord-276978-xl4u7n6r	114	30	of	of	IN
cord-276978-xl4u7n6r	114	31	a	a	DT
cord-276978-xl4u7n6r	114	32	simpler	simple	JJR
cord-276978-xl4u7n6r	114	33	RT	RT	NNP
cord-276978-xl4u7n6r	114	34	protocol	protocol	NN
cord-276978-xl4u7n6r	114	35	.	.	.
cord-276978-xl4u7n6r	115	1	7	7	CD
cord-276978-xl4u7n6r	115	2	.	.	.
cord-276978-xl4u7n6r	116	1	An	an	DT
cord-276978-xl4u7n6r	116	2	increased	increase	VBN
cord-276978-xl4u7n6r	116	3	amount	amount	NN
cord-276978-xl4u7n6r	116	4	of	of	IN
cord-276978-xl4u7n6r	116	5	the	the	DT
cord-276978-xl4u7n6r	116	6	Superscript	Superscript	NNP
cord-276978-xl4u7n6r	116	7	III	III	NNP
cord-276978-xl4u7n6r	116	8	reverse	reverse	NN
cord-276978-xl4u7n6r	116	9	transcriptase	transcriptase	NN
cord-276978-xl4u7n6r	116	10	(	(	-LRB-
cord-276978-xl4u7n6r	116	11	400	400	CD
cord-276978-xl4u7n6r	116	12	U	u	NN
cord-276978-xl4u7n6r	116	13	per	per	IN
cord-276978-xl4u7n6r	116	14	reaction	reaction	NN
cord-276978-xl4u7n6r	116	15	instead	instead	RB
cord-276978-xl4u7n6r	116	16	of	of	IN
cord-276978-xl4u7n6r	116	17	the	the	DT
cord-276978-xl4u7n6r	116	18	normally	normally	RB
cord-276978-xl4u7n6r	116	19	200	200	CD
cord-276978-xl4u7n6r	116	20	U	u	NN
cord-276978-xl4u7n6r	116	21	)	)	-RRB-
cord-276978-xl4u7n6r	116	22	is	be	VBZ
cord-276978-xl4u7n6r	116	23	used	use	VBN
cord-276978-xl4u7n6r	116	24	for	for	IN
cord-276978-xl4u7n6r	116	25	long	long	JJ
cord-276978-xl4u7n6r	116	26	-	-	HYPH
cord-276978-xl4u7n6r	116	27	range	range	NN
cord-276978-xl4u7n6r	116	28	cDNA	cdna	NN
cord-276978-xl4u7n6r	116	29	synthesis	synthesis	NN
cord-276978-xl4u7n6r	116	30	in	in	IN
cord-276978-xl4u7n6r	116	31	the	the	DT
cord-276978-xl4u7n6r	116	32	5	5	CD
cord-276978-xl4u7n6r	116	33	-RACE	-race	NN
cord-276978-xl4u7n6r	116	34	strategy	strategy	NN
cord-276978-xl4u7n6r	116	35	.	.	.
cord-276978-xl4u7n6r	117	1	In	in	IN
cord-276978-xl4u7n6r	117	2	addition	addition	NN
cord-276978-xl4u7n6r	117	3	,	,	,
cord-276978-xl4u7n6r	117	4	this	this	DT
cord-276978-xl4u7n6r	117	5	enzyme	enzyme	NN
cord-276978-xl4u7n6r	117	6	allows	allow	VBZ
cord-276978-xl4u7n6r	117	7	the	the	DT
cord-276978-xl4u7n6r	117	8	reaction	reaction	NN
cord-276978-xl4u7n6r	117	9	to	to	TO
cord-276978-xl4u7n6r	117	10	be	be	VB
cord-276978-xl4u7n6r	117	11	performed	perform	VBN
cord-276978-xl4u7n6r	117	12	at	at	IN
cord-276978-xl4u7n6r	117	13	a	a	DT
cord-276978-xl4u7n6r	117	14	temperature	temperature	NN
cord-276978-xl4u7n6r	117	15	as	as	RB
cord-276978-xl4u7n6r	117	16	high	high	JJ
cord-276978-xl4u7n6r	117	17	as	as	IN
cord-276978-xl4u7n6r	117	18	55	55	CD
cord-276978-xl4u7n6r	118	1	•	•	NNP
cord-276978-xl4u7n6r	118	2	C	C	NNP
cord-276978-xl4u7n6r	118	3	,	,	,
cord-276978-xl4u7n6r	118	4	which	which	WDT
cord-276978-xl4u7n6r	118	5	minimizes	minimize	VBZ
cord-276978-xl4u7n6r	118	6	secondary	secondary	JJ
cord-276978-xl4u7n6r	118	7	structures	structure	NNS
cord-276978-xl4u7n6r	118	8	in	in	IN
cord-276978-xl4u7n6r	118	9	RNA	RNA	NNP
cord-276978-xl4u7n6r	118	10	and	and	CC
cord-276978-xl4u7n6r	118	11	is	be	VBZ
cord-276978-xl4u7n6r	118	12	RNase	RNase	NNP
cord-276978-xl4u7n6r	118	13	H	H	NNP
cord-276978-xl4u7n6r	118	14	negative	negative	JJ
cord-276978-xl4u7n6r	118	15	,	,	,
cord-276978-xl4u7n6r	118	16	which	which	WDT
cord-276978-xl4u7n6r	118	17	increases	increase	VBZ
cord-276978-xl4u7n6r	118	18	the	the	DT
cord-276978-xl4u7n6r	118	19	yield	yield	NN
cord-276978-xl4u7n6r	118	20	of	of	IN
cord-276978-xl4u7n6r	118	21	full	full	JJ
cord-276978-xl4u7n6r	118	22	-	-	HYPH
cord-276978-xl4u7n6r	118	23	length	length	NN
cord-276978-xl4u7n6r	118	24	cDNAs	cdna	NNS
cord-276978-xl4u7n6r	118	25	.	.	.
cord-276978-xl4u7n6r	119	1	8	8	CD
cord-276978-xl4u7n6r	119	2	.	.	.
cord-276978-xl4u7n6r	120	1	It	-PRON-	PRP
cord-276978-xl4u7n6r	120	2	is	be	VBZ
cord-276978-xl4u7n6r	120	3	important	important	JJ
cord-276978-xl4u7n6r	120	4	to	to	TO
cord-276978-xl4u7n6r	120	5	degrade	degrade	VB
cord-276978-xl4u7n6r	120	6	the	the	DT
cord-276978-xl4u7n6r	120	7	template	template	NN
cord-276978-xl4u7n6r	120	8	RNA	RNA	NNP
cord-276978-xl4u7n6r	120	9	from	from	IN
cord-276978-xl4u7n6r	120	10	the	the	DT
cord-276978-xl4u7n6r	120	11	newly	newly	RB
cord-276978-xl4u7n6r	120	12	synthesized	synthesize	VBN
cord-276978-xl4u7n6r	120	13	cDNA	cdna	NN
cord-276978-xl4u7n6r	120	14	,	,	,
cord-276978-xl4u7n6r	120	15	to	to	TO
cord-276978-xl4u7n6r	120	16	avoid	avoid	VB
cord-276978-xl4u7n6r	120	17	hybrid	hybrid	JJ
cord-276978-xl4u7n6r	120	18	renaturation	renaturation	NN
cord-276978-xl4u7n6r	120	19	prior	prior	RB
cord-276978-xl4u7n6r	120	20	to	to	IN
cord-276978-xl4u7n6r	120	21	the	the	DT
cord-276978-xl4u7n6r	120	22	tailing	tailing	NN
cord-276978-xl4u7n6r	120	23	step	step	NN
cord-276978-xl4u7n6r	120	24	.	.	.
cord-276978-xl4u7n6r	121	1	The	the	DT
cord-276978-xl4u7n6r	121	2	RNase	rnase	NN
cord-276978-xl4u7n6r	121	3	mix	mix	NN
cord-276978-xl4u7n6r	121	4	consists	consist	VBZ
cord-276978-xl4u7n6r	121	5	of	of	IN
cord-276978-xl4u7n6r	121	6	both	both	DT
cord-276978-xl4u7n6r	121	7	RNase	rnase	NN
cord-276978-xl4u7n6r	121	8	H	H	NNP
cord-276978-xl4u7n6r	121	9	to	to	TO
cord-276978-xl4u7n6r	121	10	hydrolyze	hydrolyze	VB
cord-276978-xl4u7n6r	121	11	RNA	RNA	NNP
cord-276978-xl4u7n6r	121	12	bound	bind	VBN
cord-276978-xl4u7n6r	121	13	to	to	IN
cord-276978-xl4u7n6r	121	14	cDNA	cdna	NN
cord-276978-xl4u7n6r	121	15	,	,	,
cord-276978-xl4u7n6r	121	16	and	and	CC
cord-276978-xl4u7n6r	121	17	RNase	RNase	NNP
cord-276978-xl4u7n6r	121	18	T1	T1	NNP
cord-276978-xl4u7n6r	121	19	to	to	TO
cord-276978-xl4u7n6r	121	20	hydrolyze	hydrolyze	VB
cord-276978-xl4u7n6r	121	21	free	free	JJ
cord-276978-xl4u7n6r	121	22	RNA	RNA	NNP
cord-276978-xl4u7n6r	121	23	molecules	molecule	NNS
cord-276978-xl4u7n6r	121	24	.	.	.
cord-276978-xl4u7n6r	122	1	9	9	LS
cord-276978-xl4u7n6r	122	2	.	.	.
cord-276978-xl4u7n6r	123	1	The	the	DT
cord-276978-xl4u7n6r	123	2	5	5	CD
cord-276978-xl4u7n6r	123	3	-RACE	-race	NN
cord-276978-xl4u7n6r	123	4	PCR	PCR	NNP
cord-276978-xl4u7n6r	123	5	is	be	VBZ
cord-276978-xl4u7n6r	123	6	semispecific	semispecific	NNP
cord-276978-xl4u7n6r	123	7	,	,	,
cord-276978-xl4u7n6r	123	8	using	use	VBG
cord-276978-xl4u7n6r	123	9	an	an	DT
cord-276978-xl4u7n6r	123	10	anchor	anchor	NN
cord-276978-xl4u7n6r	123	11	primer	primer	NN
cord-276978-xl4u7n6r	123	12	as	as	IN
cord-276978-xl4u7n6r	123	13	sense	sense	NN
cord-276978-xl4u7n6r	123	14	primer	primer	NN
cord-276978-xl4u7n6r	123	15	,	,	,
cord-276978-xl4u7n6r	123	16	which	which	WDT
cord-276978-xl4u7n6r	123	17	amplifies	amplify	VBZ
cord-276978-xl4u7n6r	123	18	the	the	DT
cord-276978-xl4u7n6r	123	19	poly(C)-tailed	poly(C)-tailed	NNP
cord-276978-xl4u7n6r	123	20	template	template	NN
cord-276978-xl4u7n6r	123	21	,	,	,
cord-276978-xl4u7n6r	123	22	and	and	CC
cord-276978-xl4u7n6r	123	23	a	a	DT
cord-276978-xl4u7n6r	123	24	newly	newly	RB
cord-276978-xl4u7n6r	123	25	designed	design	VBN
cord-276978-xl4u7n6r	123	26	coronavirusspecific	coronavirusspecific	NNP
cord-276978-xl4u7n6r	123	27	reverse	reverse	NN
cord-276978-xl4u7n6r	123	28	primer	primer	NN
cord-276978-xl4u7n6r	123	29	.	.	.
cord-276978-xl4u7n6r	124	1	Even	even	RB
cord-276978-xl4u7n6r	124	2	if	if	IN
cord-276978-xl4u7n6r	124	3	care	care	NN
cord-276978-xl4u7n6r	124	4	has	have	VBZ
cord-276978-xl4u7n6r	124	5	been	be	VBN
cord-276978-xl4u7n6r	124	6	taken	take	VBN
cord-276978-xl4u7n6r	124	7	throughout	throughout	IN
cord-276978-xl4u7n6r	124	8	the	the	DT
cord-276978-xl4u7n6r	124	9	whole	whole	JJ
cord-276978-xl4u7n6r	124	10	procedure	procedure	NN
cord-276978-xl4u7n6r	124	11	to	to	TO
cord-276978-xl4u7n6r	124	12	avoid	avoid	VB
cord-276978-xl4u7n6r	124	13	synthesis	synthesis	NN
cord-276978-xl4u7n6r	124	14	of	of	IN
cord-276978-xl4u7n6r	124	15	short	short	JJ
cord-276978-xl4u7n6r	124	16	cDNAs	cDNAs	NNPS
cord-276978-xl4u7n6r	124	17	from	from	IN
cord-276978-xl4u7n6r	124	18	template	template	NN
cord-276978-xl4u7n6r	124	19	RNA	RNA	NNP
cord-276978-xl4u7n6r	124	20	,	,	,
cord-276978-xl4u7n6r	124	21	there	there	EX
cord-276978-xl4u7n6r	124	22	might	may	MD
cord-276978-xl4u7n6r	124	23	be	be	VB
cord-276978-xl4u7n6r	124	24	some	some	DT
cord-276978-xl4u7n6r	124	25	shorter	short	JJR
cord-276978-xl4u7n6r	124	26	cDNA	cdna	NN
cord-276978-xl4u7n6r	124	27	molecules	molecule	NNS
cord-276978-xl4u7n6r	124	28	that	that	WDT
cord-276978-xl4u7n6r	124	29	will	will	MD
cord-276978-xl4u7n6r	124	30	be	be	VB
cord-276978-xl4u7n6r	124	31	more	more	RBR
cord-276978-xl4u7n6r	124	32	easily	easily	RB
cord-276978-xl4u7n6r	124	33	amplified	amplify	VBN
cord-276978-xl4u7n6r	124	34	in	in	IN
cord-276978-xl4u7n6r	124	35	PCRs	pcr	NNS
cord-276978-xl4u7n6r	124	36	.	.	.
cord-276978-xl4u7n6r	125	1	It	-PRON-	PRP
cord-276978-xl4u7n6r	125	2	is	be	VBZ
cord-276978-xl4u7n6r	125	3	therefore	therefore	RB
cord-276978-xl4u7n6r	125	4	important	important	JJ
cord-276978-xl4u7n6r	125	5	to	to	TO
cord-276978-xl4u7n6r	125	6	use	use	VB
cord-276978-xl4u7n6r	125	7	a	a	DT
cord-276978-xl4u7n6r	125	8	PCR	PCR	NNP
cord-276978-xl4u7n6r	125	9	enzyme	enzyme	NN
cord-276978-xl4u7n6r	125	10	with	with	IN
cord-276978-xl4u7n6r	125	11	a	a	DT
cord-276978-xl4u7n6r	125	12	proofreading	proofreading	NN
cord-276978-xl4u7n6r	125	13	activity	activity	NN
cord-276978-xl4u7n6r	125	14	,	,	,
cord-276978-xl4u7n6r	125	15	in	in	IN
cord-276978-xl4u7n6r	125	16	order	order	NN
cord-276978-xl4u7n6r	125	17	to	to	TO
cord-276978-xl4u7n6r	125	18	get	get	VB
cord-276978-xl4u7n6r	125	19	PCR	PCR	NNP
cord-276978-xl4u7n6r	125	20	products	product	NNS
cord-276978-xl4u7n6r	125	21	from	from	IN
cord-276978-xl4u7n6r	125	22	the	the	DT
cord-276978-xl4u7n6r	125	23	longer	long	JJR
cord-276978-xl4u7n6r	125	24	cDNA	cdna	NN
cord-276978-xl4u7n6r	125	25	molecules	molecule	NNS
cord-276978-xl4u7n6r	125	26	as	as	RB
cord-276978-xl4u7n6r	125	27	well	well	RB
cord-276978-xl4u7n6r	125	28	.	.	.
cord-276978-xl4u7n6r	126	1	10	10	CD
cord-276978-xl4u7n6r	126	2	.	.	.
cord-276978-xl4u7n6r	127	1	The	the	DT
cord-276978-xl4u7n6r	127	2	long	long	JJ
cord-276978-xl4u7n6r	127	3	elongation	elongation	NN
cord-276978-xl4u7n6r	127	4	step	step	NN
cord-276978-xl4u7n6r	127	5	is	be	VBZ
cord-276978-xl4u7n6r	127	6	chosen	choose	VBN
cord-276978-xl4u7n6r	127	7	to	to	TO
cord-276978-xl4u7n6r	127	8	allow	allow	VB
cord-276978-xl4u7n6r	127	9	amplification	amplification	NN
cord-276978-xl4u7n6r	127	10	of	of	IN
cord-276978-xl4u7n6r	127	11	longer	long	JJR
cord-276978-xl4u7n6r	127	12	cDNAs	cdnas	ADD
cord-276978-xl4u7n6r	127	13	.	.	.
cord-276978-xl4u7n6r	128	1	11	11	CD
cord-276978-xl4u7n6r	128	2	.	.	.
cord-276978-xl4u7n6r	129	1	As	as	IN
cord-276978-xl4u7n6r	129	2	cDNA	cdna	NN
cord-276978-xl4u7n6r	129	3	molecules	molecule	NNS
cord-276978-xl4u7n6r	129	4	of	of	IN
cord-276978-xl4u7n6r	129	5	different	different	JJ
cord-276978-xl4u7n6r	129	6	lengths	length	NNS
cord-276978-xl4u7n6r	129	7	are	be	VBP
cord-276978-xl4u7n6r	129	8	generated	generate	VBN
cord-276978-xl4u7n6r	129	9	during	during	IN
cord-276978-xl4u7n6r	129	10	RT	RT	NNP
cord-276978-xl4u7n6r	129	11	,	,	,
cord-276978-xl4u7n6r	129	12	the	the	DT
cord-276978-xl4u7n6r	129	13	5	5	CD
cord-276978-xl4u7n6r	129	14	-RACE	-RACE	.
cord-276978-xl4u7n6r	130	1	PCR	PCR	NNP
cord-276978-xl4u7n6r	130	2	products	product	NNS
cord-276978-xl4u7n6r	130	3	will	will	MD
cord-276978-xl4u7n6r	130	4	be	be	VB
cord-276978-xl4u7n6r	130	5	of	of	IN
cord-276978-xl4u7n6r	130	6	different	different	JJ
cord-276978-xl4u7n6r	130	7	lengths	length	NNS
cord-276978-xl4u7n6r	130	8	,	,	,
cord-276978-xl4u7n6r	130	9	so	so	CC
cord-276978-xl4u7n6r	130	10	the	the	DT
cord-276978-xl4u7n6r	130	11	5	5	CD
cord-276978-xl4u7n6r	130	12	-RACE	-race	RB
cord-276978-xl4u7n6r	130	13	abridged	abridge	VBN
cord-276978-xl4u7n6r	130	14	anchor	anchor	NN
cord-276978-xl4u7n6r	130	15	primer	primer	NN
cord-276978-xl4u7n6r	130	16	can	can	MD
cord-276978-xl4u7n6r	130	17	not	not	RB
cord-276978-xl4u7n6r	130	18	be	be	VB
cord-276978-xl4u7n6r	130	19	used	use	VBN
cord-276978-xl4u7n6r	130	20	in	in	IN
cord-276978-xl4u7n6r	130	21	cycle	cycle	NN
cord-276978-xl4u7n6r	130	22	sequencing	sequencing	NN
cord-276978-xl4u7n6r	130	23	.	.	.
cord-276978-xl4u7n6r	131	1	As	as	IN
cord-276978-xl4u7n6r	131	2	all	all	PDT
cord-276978-xl4u7n6r	131	3	the	the	DT
cord-276978-xl4u7n6r	131	4	5	5	CD
cord-276978-xl4u7n6r	131	5	-RACE	-RACE	.
cord-276978-xl4u7n6r	132	1	PCR	PCR	NNP
cord-276978-xl4u7n6r	132	2	products	product	NNS
cord-276978-xl4u7n6r	132	3	have	have	VBP
cord-276978-xl4u7n6r	132	4	aligned	align	VBN
cord-276978-xl4u7n6r	132	5	3	3	CD
cord-276978-xl4u7n6r	132	6	-ends	-end	NNS
cord-276978-xl4u7n6r	132	7	,	,	,
cord-276978-xl4u7n6r	133	1	the	the	DT
cord-276978-xl4u7n6r	133	2	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	133	3	-	-	HYPH
cord-276978-xl4u7n6r	133	4	specific	specific	JJ
cord-276978-xl4u7n6r	133	5	5	5	CD
cord-276978-xl4u7n6r	133	6	-RACE	-race	RB
cord-276978-xl4u7n6r	133	7	reverse	reverse	JJ
cord-276978-xl4u7n6r	133	8	PCR	PCR	NNP
cord-276978-xl4u7n6r	133	9	primer	primer	NN
cord-276978-xl4u7n6r	133	10	or	or	CC
cord-276978-xl4u7n6r	133	11	,	,	,
cord-276978-xl4u7n6r	133	12	preferably	preferably	RB
cord-276978-xl4u7n6r	133	13	for	for	IN
cord-276978-xl4u7n6r	133	14	increased	increase	VBN
cord-276978-xl4u7n6r	133	15	specificity	specificity	NN
cord-276978-xl4u7n6r	133	16	,	,	,
cord-276978-xl4u7n6r	133	17	a	a	DT
cord-276978-xl4u7n6r	133	18	primer	primer	NN
cord-276978-xl4u7n6r	133	19	slightly	slightly	RB
cord-276978-xl4u7n6r	133	20	upstream	upstream	RB
cord-276978-xl4u7n6r	133	21	of	of	IN
cord-276978-xl4u7n6r	133	22	this	this	DT
cord-276978-xl4u7n6r	133	23	PCR	PCR	NNP
cord-276978-xl4u7n6r	133	24	primer	primer	NN
cord-276978-xl4u7n6r	133	25	is	be	VBZ
cord-276978-xl4u7n6r	133	26	used	use	VBN
cord-276978-xl4u7n6r	133	27	in	in	IN
cord-276978-xl4u7n6r	133	28	cycle	cycle	NN
cord-276978-xl4u7n6r	133	29	sequencing	sequencing	NN
cord-276978-xl4u7n6r	133	30	.	.	.
cord-276978-xl4u7n6r	134	1	12	12	CD
cord-276978-xl4u7n6r	134	2	.	.	.
cord-276978-xl4u7n6r	135	1	Using	use	VBG
cord-276978-xl4u7n6r	135	2	this	this	DT
cord-276978-xl4u7n6r	135	3	procedure	procedure	NN
cord-276978-xl4u7n6r	135	4	,	,	,
cord-276978-xl4u7n6r	135	5	one	one	PRP
cord-276978-xl4u7n6r	135	6	should	should	MD
cord-276978-xl4u7n6r	135	7	obtain	obtain	VB
cord-276978-xl4u7n6r	135	8	at	at	IN
cord-276978-xl4u7n6r	135	9	least	least	RBS
cord-276978-xl4u7n6r	135	10	500	500	CD
cord-276978-xl4u7n6r	135	11	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	135	12	,	,	,
cord-276978-xl4u7n6r	135	13	which	which	WDT
cord-276978-xl4u7n6r	135	14	would	would	MD
cord-276978-xl4u7n6r	135	15	allow	allow	VB
cord-276978-xl4u7n6r	135	16	the	the	DT
cord-276978-xl4u7n6r	135	17	design	design	NN
cord-276978-xl4u7n6r	135	18	of	of	IN
cord-276978-xl4u7n6r	135	19	new	new	JJ
cord-276978-xl4u7n6r	135	20	primers	primer	NNS
cord-276978-xl4u7n6r	135	21	both	both	CC
cord-276978-xl4u7n6r	135	22	in	in	IN
cord-276978-xl4u7n6r	135	23	sense	sense	NN
cord-276978-xl4u7n6r	135	24	direction	direction	NN
cord-276978-xl4u7n6r	135	25	for	for	IN
cord-276978-xl4u7n6r	135	26	sequence	sequence	NN
cord-276978-xl4u7n6r	135	27	verification	verification	NN
cord-276978-xl4u7n6r	135	28	and	and	CC
cord-276978-xl4u7n6r	135	29	reverse	reverse	JJ
cord-276978-xl4u7n6r	135	30	direction	direction	NN
cord-276978-xl4u7n6r	135	31	for	for	IN
cord-276978-xl4u7n6r	135	32	acquisition	acquisition	NN
cord-276978-xl4u7n6r	135	33	of	of	IN
cord-276978-xl4u7n6r	135	34	new	new	JJ
cord-276978-xl4u7n6r	135	35	sequence	sequence	NN
cord-276978-xl4u7n6r	135	36	data	datum	NNS
cord-276978-xl4u7n6r	135	37	toward	toward	IN
cord-276978-xl4u7n6r	135	38	the	the	DT
cord-276978-xl4u7n6r	135	39	5	5	CD
cord-276978-xl4u7n6r	135	40	-end	-end	:
cord-276978-xl4u7n6r	135	41	of	of	IN
cord-276978-xl4u7n6r	135	42	the	the	DT
cord-276978-xl4u7n6r	135	43	genome	genome	NN
cord-276978-xl4u7n6r	135	44	in	in	IN
cord-276978-xl4u7n6r	135	45	a	a	DT
cord-276978-xl4u7n6r	135	46	primer	primer	NN
cord-276978-xl4u7n6r	135	47	walking	walking	NN
cord-276978-xl4u7n6r	135	48	strategy	strategy	NN
cord-276978-xl4u7n6r	135	49	.	.	.
cord-276978-xl4u7n6r	136	1	The	the	DT
cord-276978-xl4u7n6r	136	2	new	new	JJ
cord-276978-xl4u7n6r	136	3	primers	primer	NNS
cord-276978-xl4u7n6r	136	4	can	can	MD
cord-276978-xl4u7n6r	136	5	be	be	VB
cord-276978-xl4u7n6r	136	6	used	use	VBN
cord-276978-xl4u7n6r	136	7	directly	directly	RB
cord-276978-xl4u7n6r	136	8	in	in	IN
cord-276978-xl4u7n6r	136	9	step	step	NN
cord-276978-xl4u7n6r	136	10	5	5	CD
cord-276978-xl4u7n6r	136	11	of	of	IN
cord-276978-xl4u7n6r	136	12	the	the	DT
cord-276978-xl4u7n6r	136	13	5	5	CD
cord-276978-xl4u7n6r	136	14	-RACE	-race	NN
cord-276978-xl4u7n6r	136	15	procedure	procedure	NN
cord-276978-xl4u7n6r	136	16	,	,	,
cord-276978-xl4u7n6r	136	17	if	if	IN
cord-276978-xl4u7n6r	136	18	all	all	PDT
cord-276978-xl4u7n6r	136	19	the	the	DT
cord-276978-xl4u7n6r	136	20	steps	step	NNS
cord-276978-xl4u7n6r	136	21	have	have	VBP
cord-276978-xl4u7n6r	136	22	been	be	VBN
cord-276978-xl4u7n6r	136	23	optimized	optimize	VBN
cord-276978-xl4u7n6r	136	24	for	for	IN
cord-276978-xl4u7n6r	136	25	longrange	longrange	NNP
cord-276978-xl4u7n6r	136	26	cDNA	cDNA	NNP
cord-276978-xl4u7n6r	136	27	synthesis	synthesis	NNP
cord-276978-xl4u7n6r	136	28	.	.	.
cord-276978-xl4u7n6r	137	1	However	however	RB
cord-276978-xl4u7n6r	137	2	,	,	,
cord-276978-xl4u7n6r	137	3	usually	usually	RB
cord-276978-xl4u7n6r	137	4	no	no	DT
cord-276978-xl4u7n6r	137	5	more	more	JJR
cord-276978-xl4u7n6r	137	6	than	than	IN
cord-276978-xl4u7n6r	137	7	2000	2000	CD
cord-276978-xl4u7n6r	137	8	-	-	SYM
cord-276978-xl4u7n6r	137	9	3000	3000	CD
cord-276978-xl4u7n6r	137	10	nucleotides	nucleotide	NNS
cord-276978-xl4u7n6r	137	11	are	be	VBP
cord-276978-xl4u7n6r	137	12	efficiently	efficiently	RB
cord-276978-xl4u7n6r	137	13	sequenced	sequence	VBN
cord-276978-xl4u7n6r	137	14	from	from	IN
cord-276978-xl4u7n6r	137	15	the	the	DT
cord-276978-xl4u7n6r	137	16	initial	initial	JJ
cord-276978-xl4u7n6r	137	17	5	5	CD
cord-276978-xl4u7n6r	137	18	-RACE	-race	NN
cord-276978-xl4u7n6r	137	19	-	-	HYPH
cord-276978-xl4u7n6r	137	20	RT	RT	NNP
cord-276978-xl4u7n6r	137	21	,	,	,
cord-276978-xl4u7n6r	137	22	and	and	CC
cord-276978-xl4u7n6r	137	23	the	the	DT
cord-276978-xl4u7n6r	137	24	whole	whole	JJ
cord-276978-xl4u7n6r	137	25	5	5	CD
cord-276978-xl4u7n6r	137	26	-RACE	-race	NN
cord-276978-xl4u7n6r	137	27	procedure	procedure	NN
cord-276978-xl4u7n6r	137	28	has	have	VBZ
cord-276978-xl4u7n6r	137	29	to	to	TO
cord-276978-xl4u7n6r	137	30	be	be	VB
cord-276978-xl4u7n6r	137	31	repeated	repeat	VBN
cord-276978-xl4u7n6r	137	32	with	with	IN
cord-276978-xl4u7n6r	137	33	a	a	DT
cord-276978-xl4u7n6r	137	34	primer	primer	NN
cord-276978-xl4u7n6r	137	35	for	for	IN
cord-276978-xl4u7n6r	137	36	cDNA	cdna	NN
cord-276978-xl4u7n6r	137	37	synthesis	synthesis	NN
cord-276978-xl4u7n6r	137	38	as	as	RB
cord-276978-xl4u7n6r	137	39	upstream	upstream	RB
cord-276978-xl4u7n6r	137	40	as	as	IN
cord-276978-xl4u7n6r	137	41	possible	possible	JJ
cord-276978-xl4u7n6r	137	42	.	.	.
cord-276978-xl4u7n6r	138	1	BigDye	BigDye	NNP
cord-276978-xl4u7n6r	138	2	Terminator	Terminator	NNP
cord-276978-xl4u7n6r	138	3	Cycle	Cycle	NNP
cord-276978-xl4u7n6r	138	4	Sequencing	Sequencing	NNP
cord-276978-xl4u7n6r	138	5	kit	kit	NN
cord-276978-xl4u7n6r	138	6	version	version	NN
cord-276978-xl4u7n6r	138	7	1.1	1.1	CD
cord-276978-xl4u7n6r	138	8	or	or	CC
cord-276978-xl4u7n6r	138	9	version	version	NN
cord-276978-xl4u7n6r	138	10	3.1	3.1	CD
cord-276978-xl4u7n6r	138	11	(	(	-LRB-
cord-276978-xl4u7n6r	138	12	Applied	Applied	NNP
cord-276978-xl4u7n6r	138	13	Biosystems	Biosystems	NNP
cord-276978-xl4u7n6r	138	14	)	)	-RRB-
cord-276978-xl4u7n6r	138	15	is	be	VBZ
cord-276978-xl4u7n6r	138	16	used	use	VBN
cord-276978-xl4u7n6r	138	17	for	for	IN
cord-276978-xl4u7n6r	138	18	sequencing	sequencing	NN
cord-276978-xl4u7n6r	138	19	of	of	IN
cord-276978-xl4u7n6r	138	20	short	short	JJ
cord-276978-xl4u7n6r	138	21	(	(	-LRB-
cord-276978-xl4u7n6r	138	22	from	from	IN
cord-276978-xl4u7n6r	138	23	initial	initial	JJ
cord-276978-xl4u7n6r	138	24	PCR	PCR	NNP
cord-276978-xl4u7n6r	138	25	)	)	-RRB-
cord-276978-xl4u7n6r	138	26	or	or	CC
cord-276978-xl4u7n6r	138	27	long	long	JJ
cord-276978-xl4u7n6r	138	28	(	(	-LRB-
cord-276978-xl4u7n6r	138	29	5	5	CD
cord-276978-xl4u7n6r	138	30	-RACE	-race	NN
cord-276978-xl4u7n6r	138	31	PCR	PCR	NNP
cord-276978-xl4u7n6r	138	32	)	)	-RRB-
cord-276978-xl4u7n6r	138	33	PCR	PCR	NNP
cord-276978-xl4u7n6r	138	34	products	product	NNS
cord-276978-xl4u7n6r	138	35	Cycle	cycle	NN
cord-276978-xl4u7n6r	138	36	sequencing	sequencing	NN
cord-276978-xl4u7n6r	138	37	reaction	reaction	NN
cord-276978-xl4u7n6r	138	38	is	be	VBZ
cord-276978-xl4u7n6r	138	39	performed	perform	VBN
cord-276978-xl4u7n6r	138	40	on	on	IN
cord-276978-xl4u7n6r	138	41	an	an	DT
cord-276978-xl4u7n6r	138	42	MJ	MJ	NNP
cord-276978-xl4u7n6r	138	43	Research	Research	NNP
cord-276978-xl4u7n6r	138	44	thermocycler	thermocycler	VBD
cord-276978-xl4u7n6r	138	45	3130xl	3130xl	CD
cord-276978-xl4u7n6r	138	46	Genetic	Genetic	NNP
cord-276978-xl4u7n6r	138	47	Analyser	Analyser	NNP
cord-276978-xl4u7n6r	138	48	for	for	IN
cord-276978-xl4u7n6r	138	49	sequencing	sequencing	NN
cord-276978-xl4u7n6r	138	50	(	(	-LRB-
cord-276978-xl4u7n6r	138	51	Applied	Applied	NNP
cord-276978-xl4u7n6r	138	52	Biosystems	Biosystems	NNP
cord-276978-xl4u7n6r	138	53	)	)	-RRB-
cord-276978-xl4u7n6r	139	1	All	all	DT
cord-276978-xl4u7n6r	139	2	reagents	reagent	NNS
cord-276978-xl4u7n6r	139	3	for	for	IN
cord-276978-xl4u7n6r	139	4	RT	RT	NNP
cord-276978-xl4u7n6r	139	5	are	be	VBP
cord-276978-xl4u7n6r	139	6	described	describe	VBN
cord-276978-xl4u7n6r	139	7	in	in	IN
cord-276978-xl4u7n6r	139	8	Section	section	NN
cord-276978-xl4u7n6r	139	9	2.1.1	2.1.1	NN
cord-276978-xl4u7n6r	139	10	For	for	IN
cord-276978-xl4u7n6r	139	11	RNase	rnase	NN
cord-276978-xl4u7n6r	139	12	treatment	treatment	NN
cord-276978-xl4u7n6r	139	13	of	of	IN
cord-276978-xl4u7n6r	139	14	newly	newly	RB
cord-276978-xl4u7n6r	139	15	synthesized	synthesize	VBN
cord-276978-xl4u7n6r	139	16	cDNA	cdna	NN
cord-276978-xl4u7n6r	139	17	,	,	,
cord-276978-xl4u7n6r	139	18	a	a	DT
cord-276978-xl4u7n6r	139	19	master	master	NN
cord-276978-xl4u7n6r	139	20	mix	mix	NN
cord-276978-xl4u7n6r	139	21	of	of	IN
cord-276978-xl4u7n6r	139	22	two	two	CD
cord-276978-xl4u7n6r	139	23	-	-	HYPH
cord-276978-xl4u7n6r	139	24	thirds	third	NNS
cord-276978-xl4u7n6r	139	25	part	part	NN
cord-276978-xl4u7n6r	139	26	RNase	RNase	NNP
cord-276978-xl4u7n6r	139	27	H	h	NN
cord-276978-xl4u7n6r	139	28	(	(	-LRB-
cord-276978-xl4u7n6r	139	29	2	2	CD
cord-276978-xl4u7n6r	139	30	U	u	NN
cord-276978-xl4u7n6r	139	31	/	/	SYM
cord-276978-xl4u7n6r	139	32	l	l	NN
cord-276978-xl4u7n6r	139	33	)	)	-RRB-
cord-276978-xl4u7n6r	139	34	(	(	-LRB-
cord-276978-xl4u7n6r	139	35	Invitrogen	Invitrogen	NNP
cord-276978-xl4u7n6r	139	36	)	)	-RRB-
cord-276978-xl4u7n6r	139	37	and	and	CC
cord-276978-xl4u7n6r	139	38	one	one	CD
cord-276978-xl4u7n6r	139	39	-	-	HYPH
cord-276978-xl4u7n6r	139	40	third	third	NN
cord-276978-xl4u7n6r	139	41	part	part	NN
cord-276978-xl4u7n6r	139	42	RNase	RNase	NNP
cord-276978-xl4u7n6r	139	43	T1	T1	NNP
cord-276978-xl4u7n6r	139	44	(	(	-LRB-
cord-276978-xl4u7n6r	139	45	100	100	CD
cord-276978-xl4u7n6r	139	46	-	-	SYM
cord-276978-xl4u7n6r	139	47	150	150	CD
cord-276978-xl4u7n6r	139	48	U	U	NNP
cord-276978-xl4u7n6r	139	49	/	/	SYM
cord-276978-xl4u7n6r	139	50	l	l	NN
cord-276978-xl4u7n6r	139	51	)	)	-RRB-
cord-276978-xl4u7n6r	139	52	(	(	-LRB-
cord-276978-xl4u7n6r	139	53	Roche	Roche	NNP
cord-276978-xl4u7n6r	139	54	)	)	-RRB-
cord-276978-xl4u7n6r	139	55	is	be	VBZ
cord-276978-xl4u7n6r	139	56	made	make	VBN
cord-276978-xl4u7n6r	139	57	.	.	.
cord-276978-xl4u7n6r	140	1	The	the	DT
cord-276978-xl4u7n6r	140	2	mix	mix	NN
cord-276978-xl4u7n6r	140	3	is	be	VBZ
cord-276978-xl4u7n6r	140	4	made	make	VBN
cord-276978-xl4u7n6r	140	5	fresh	fresh	JJ
cord-276978-xl4u7n6r	140	6	,	,	,
cord-276978-xl4u7n6r	140	7	and	and	CC
cord-276978-xl4u7n6r	140	8	stored	store	VBN
cord-276978-xl4u7n6r	140	9	at	at	IN
cord-276978-xl4u7n6r	140	10	4	4	CD
cord-276978-xl4u7n6r	140	11	•	•	NN
cord-276978-xl4u7n6r	140	12	C	C	NNP
cord-276978-xl4u7n6r	140	13	until	until	IN
cord-276978-xl4u7n6r	140	14	use	use	NN
cord-276978-xl4u7n6r	140	15	QIAquick	QIAquick	NNP
cord-276978-xl4u7n6r	140	16	PCR	PCR	NNP
cord-276978-xl4u7n6r	140	17	purification	purification	NN
cord-276978-xl4u7n6r	140	18	kit	kit	NN
cord-276978-xl4u7n6r	140	19	(	(	-LRB-
cord-276978-xl4u7n6r	140	20	Qiagen	Qiagen	NNP
cord-276978-xl4u7n6r	140	21	)	)	-RRB-
cord-276978-xl4u7n6r	141	1	cDNA	cDNA	NNP
cord-276978-xl4u7n6r	141	2	yields	yield	NNS
cord-276978-xl4u7n6r	141	3	are	be	VBP
cord-276978-xl4u7n6r	141	4	measured	measure	VBN
cord-276978-xl4u7n6r	141	5	using	use	VBG
cord-276978-xl4u7n6r	141	6	a	a	DT
cord-276978-xl4u7n6r	141	7	NanoDrop	NanoDrop	NNP
cord-276978-xl4u7n6r	141	8	Spectrophotometer	Spectrophotometer	NNP
cord-276978-xl4u7n6r	141	9	Tailing	tailing	NN
cord-276978-xl4u7n6r	141	10	reagents	reagent	NNS
cord-276978-xl4u7n6r	141	11	:	:	:
cord-276978-xl4u7n6r	141	12	Terminal	terminal	JJ
cord-276978-xl4u7n6r	141	13	deoxynucleotidyl	deoxynucleotidyl	NN
cord-276978-xl4u7n6r	141	14	transferase	transferase	NN
cord-276978-xl4u7n6r	141	15	(	(	-LRB-
cord-276978-xl4u7n6r	141	16	TdT	TdT	NNP
cord-276978-xl4u7n6r	141	17	)	)	-RRB-
cord-276978-xl4u7n6r	141	18	(	(	-LRB-
cord-276978-xl4u7n6r	141	19	15	15	CD
cord-276978-xl4u7n6r	141	20	U	U	NNP
cord-276978-xl4u7n6r	141	21	/	/	SYM
cord-276978-xl4u7n6r	141	22	l	l	NN
cord-276978-xl4u7n6r	141	23	)	)	-RRB-
cord-276978-xl4u7n6r	141	24	,	,	,
cord-276978-xl4u7n6r	141	25	5X	5x	CD
cord-276978-xl4u7n6r	141	26	TdT	tdt	NN
cord-276978-xl4u7n6r	141	27	buffer	buffer	NN
cord-276978-xl4u7n6r	141	28	(	(	-LRB-
cord-276978-xl4u7n6r	141	29	0.5	0.5	CD
cord-276978-xl4u7n6r	141	30	M	m	NN
cord-276978-xl4u7n6r	141	31	potassium	potassium	NN
cord-276978-xl4u7n6r	141	32	cacodylate	cacodylate	NN
cord-276978-xl4u7n6r	141	33	,	,	,
cord-276978-xl4u7n6r	141	34	10	10	CD
cord-276978-xl4u7n6r	141	35	mM	mm	CD
cord-276978-xl4u7n6r	141	36	CoCl	CoCl	NNP
cord-276978-xl4u7n6r	141	37	2	2	CD
cord-276978-xl4u7n6r	141	38	,	,	,
cord-276978-xl4u7n6r	141	39	1	1	CD
cord-276978-xl4u7n6r	141	40	mM	mm	CD
cord-276978-xl4u7n6r	141	41	DTT	DTT	NNP
cord-276978-xl4u7n6r	141	42	)	)	-RRB-
cord-276978-xl4u7n6r	141	43	,	,	,
cord-276978-xl4u7n6r	141	44	and	and	CC
cord-276978-xl4u7n6r	141	45	dCTP	dctp	JJ
cord-276978-xl4u7n6r	141	46	Sense	sense	NN
cord-276978-xl4u7n6r	141	47	primer	primer	NN
cord-276978-xl4u7n6r	141	48	in	in	IN
cord-276978-xl4u7n6r	141	49	the	the	DT
cord-276978-xl4u7n6r	141	50	5	5	CD
cord-276978-xl4u7n6r	141	51	-RACE	-race	NN
cord-276978-xl4u7n6r	141	52	PCR	PCR	NNP
cord-276978-xl4u7n6r	141	53	:	:	:
cord-276978-xl4u7n6r	141	54	5	5	LS
cord-276978-xl4u7n6r	141	55	-RACE	-race	RB
cord-276978-xl4u7n6r	141	56	abridged	abridge	VBN
cord-276978-xl4u7n6r	141	57	anchor	anchor	NN
cord-276978-xl4u7n6r	141	58	primer	primer	NN
cord-276978-xl4u7n6r	141	59	PCR	PCR	NNP
cord-276978-xl4u7n6r	141	60	enzyme	enzyme	NN
cord-276978-xl4u7n6r	141	61	used	use	VBN
cord-276978-xl4u7n6r	141	62	in	in	IN
cord-276978-xl4u7n6r	141	63	the	the	DT
cord-276978-xl4u7n6r	141	64	long	long	JJ
cord-276978-xl4u7n6r	141	65	-	-	HYPH
cord-276978-xl4u7n6r	141	66	range	range	NN
cord-276978-xl4u7n6r	141	67	5	5	CD
cord-276978-xl4u7n6r	141	68	-RACE	-race	NN
cord-276978-xl4u7n6r	141	69	PCR	PCR	NNP
cord-276978-xl4u7n6r	141	70	:	:	:
cord-276978-xl4u7n6r	141	71	BD	BD	NNP
cord-276978-xl4u7n6r	141	72	Advantage	Advantage	NNP
cord-276978-xl4u7n6r	141	73	2	2	CD
cord-276978-xl4u7n6r	141	74	Taq	taq	NN
cord-276978-xl4u7n6r	141	75	polymerase	polymerase	NN
cord-276978-xl4u7n6r	141	76	with	with	IN
cord-276978-xl4u7n6r	141	77	10X	10X	JJR
cord-276978-xl4u7n6r	141	78	BD	BD	NNP
cord-276978-xl4u7n6r	141	79	Advantage	Advantage	NNP
cord-276978-xl4u7n6r	141	80	2	2	CD
cord-276978-xl4u7n6r	141	81	PCR	PCR	NNP
cord-276978-xl4u7n6r	141	82	buffer	buffer	NN
cord-276978-xl4u7n6r	141	83	(	(	-LRB-
cord-276978-xl4u7n6r	141	84	400	400	CD
cord-276978-xl4u7n6r	141	85	mM	mm	CD
cord-276978-xl4u7n6r	141	86	Tricine	Tricine	NNP
cord-276978-xl4u7n6r	141	87	-	-	HYPH
cord-276978-xl4u7n6r	141	88	KOH	KOH	NNP
cord-276978-xl4u7n6r	141	89	,	,	,
cord-276978-xl4u7n6r	141	90	150	150	CD
cord-276978-xl4u7n6r	141	91	mM	mm	CD
cord-276978-xl4u7n6r	141	92	KOAc	KOAc	NNS
cord-276978-xl4u7n6r	141	93	,	,	,
cord-276978-xl4u7n6r	141	94	35	35	CD
cord-276978-xl4u7n6r	141	95	mM	mm	CD
cord-276978-xl4u7n6r	141	96	Mg(OAc	Mg(OAc	NNS
cord-276978-xl4u7n6r	141	97	)	)	-RRB-
cord-276978-xl4u7n6r	141	98	2	2	CD
cord-276978-xl4u7n6r	141	99	,	,	,
cord-276978-xl4u7n6r	141	100	37.5	37.5	CD
cord-276978-xl4u7n6r	141	101	g	g	NN
cord-276978-xl4u7n6r	141	102	/	/	SYM
cord-276978-xl4u7n6r	141	103	l	l	NNP
cord-276978-xl4u7n6r	141	104	BSA	BSA	NNP
cord-276978-xl4u7n6r	141	105	,	,	,
cord-276978-xl4u7n6r	141	106	0.05	0.05	CD
cord-276978-xl4u7n6r	141	107	%	%	NN
cord-276978-xl4u7n6r	141	108	Tween	Tween	NNP
cord-276978-xl4u7n6r	141	109	20	20	CD
cord-276978-xl4u7n6r	141	110	,	,	,
cord-276978-xl4u7n6r	141	111	and	and	CC
cord-276978-xl4u7n6r	141	112	0.05	0.05	CD
cord-276978-xl4u7n6r	141	113	%	%	NN
cord-276978-xl4u7n6r	141	114	Nonidet	Nonidet	NNP
cord-276978-xl4u7n6r	141	115	-	-	HYPH
cord-276978-xl4u7n6r	141	116	P40	P40	NNP
cord-276978-xl4u7n6r	141	117	)	)	-RRB-
cord-276978-xl4u7n6r	141	118	(	(	-LRB-
cord-276978-xl4u7n6r	141	119	Clontech	Clontech	NNP
cord-276978-xl4u7n6r	141	120	)	)	-RRB-
cord-276978-xl4u7n6r	142	1	A	a	DT
cord-276978-xl4u7n6r	142	2	common	common	JJ
cord-276978-xl4u7n6r	142	3	RNA	RNA	NNP
cord-276978-xl4u7n6r	142	4	motif	motif	NN
cord-276978-xl4u7n6r	142	5	in	in	IN
cord-276978-xl4u7n6r	142	6	the	the	DT
cord-276978-xl4u7n6r	142	7	3	3	CD
cord-276978-xl4u7n6r	142	8	'	'	NN
cord-276978-xl4u7n6r	142	9	end	end	NN
cord-276978-xl4u7n6r	142	10	of	of	IN
cord-276978-xl4u7n6r	142	11	the	the	DT
cord-276978-xl4u7n6r	142	12	genomes	genome	NNS
cord-276978-xl4u7n6r	142	13	of	of	IN
cord-276978-xl4u7n6r	142	14	astroviruses	astroviruse	NNS
cord-276978-xl4u7n6r	142	15	,	,	,
cord-276978-xl4u7n6r	142	16	avian	avian	JJ
cord-276978-xl4u7n6r	142	17	infectious	infectious	JJ
cord-276978-xl4u7n6r	142	18	bronchitis	bronchitis	NN
cord-276978-xl4u7n6r	142	19	virus	virus	NN
cord-276978-xl4u7n6r	142	20	and	and	CC
cord-276978-xl4u7n6r	142	21	an	an	DT
cord-276978-xl4u7n6r	142	22	equine	equine	JJ
cord-276978-xl4u7n6r	142	23	rhinovirus	rhinovirus	NN
cord-276978-xl4u7n6r	143	1	The	the	DT
cord-276978-xl4u7n6r	143	2	structure	structure	NN
cord-276978-xl4u7n6r	143	3	of	of	IN
cord-276978-xl4u7n6r	143	4	a	a	DT
cord-276978-xl4u7n6r	143	5	rigorously	rigorously	RB
cord-276978-xl4u7n6r	143	6	conserved	conserve	VBN
cord-276978-xl4u7n6r	143	7	RNA	RNA	NNP
cord-276978-xl4u7n6r	143	8	element	element	NN
cord-276978-xl4u7n6r	143	9	within	within	IN
cord-276978-xl4u7n6r	143	10	the	the	DT
cord-276978-xl4u7n6r	143	11	SARS	SARS	NNP
cord-276978-xl4u7n6r	143	12	virus	virus	NN
cord-276978-xl4u7n6r	143	13	genome	genome	NN
cord-276978-xl4u7n6r	143	14	Molecular	molecular	JJ
cord-276978-xl4u7n6r	143	15	identification	identification	NN
cord-276978-xl4u7n6r	143	16	and	and	CC
cord-276978-xl4u7n6r	143	17	characterization	characterization	NN
cord-276978-xl4u7n6r	143	18	of	of	IN
cord-276978-xl4u7n6r	143	19	novel	novel	NN
cord-276978-xl4u7n6r	143	20	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	143	21	infecting	infect	VBG
cord-276978-xl4u7n6r	143	22	graylag	graylag	NNP
cord-276978-xl4u7n6r	143	23	geese	goose	NNS
cord-276978-xl4u7n6r	143	24	(	(	-LRB-
cord-276978-xl4u7n6r	143	25	Anser	Anser	NNP
cord-276978-xl4u7n6r	143	26	anser	anser	NNP
cord-276978-xl4u7n6r	143	27	)	)	-RRB-
cord-276978-xl4u7n6r	143	28	,	,	,
cord-276978-xl4u7n6r	143	29	feral	feral	JJ
cord-276978-xl4u7n6r	143	30	pigeons	pigeon	NNS
cord-276978-xl4u7n6r	143	31	(	(	-LRB-
cord-276978-xl4u7n6r	143	32	Columbia	Columbia	NNP
cord-276978-xl4u7n6r	143	33	livia	livia	NNP
cord-276978-xl4u7n6r	143	34	)	)	-RRB-
cord-276978-xl4u7n6r	143	35	and	and	CC
cord-276978-xl4u7n6r	143	36	mallards	mallard	NNS
cord-276978-xl4u7n6r	143	37	(	(	-LRB-
cord-276978-xl4u7n6r	143	38	Anas	Anas	NNP
cord-276978-xl4u7n6r	143	39	platyrhynchos	platyrhynchos	NN
cord-276978-xl4u7n6r	143	40	)	)	-RRB-
cord-276978-xl4u7n6r	144	1	A	a	DT
cord-276978-xl4u7n6r	144	2	novel	novel	JJ
cord-276978-xl4u7n6r	144	3	coronavirus	coronavirus	NN
cord-276978-xl4u7n6r	144	4	associated	associate	VBN
cord-276978-xl4u7n6r	144	5	with	with	IN
cord-276978-xl4u7n6r	144	6	severe	severe	JJ
cord-276978-xl4u7n6r	144	7	acute	acute	JJ
cord-276978-xl4u7n6r	144	8	respiratory	respiratory	JJ
cord-276978-xl4u7n6r	144	9	syndrome	syndrome	NN
cord-276978-xl4u7n6r	144	10	Viral	viral	JJ
cord-276978-xl4u7n6r	144	11	discovery	discovery	NN
cord-276978-xl4u7n6r	144	12	and	and	CC
cord-276978-xl4u7n6r	144	13	sequence	sequence	NN
cord-276978-xl4u7n6r	144	14	recovery	recovery	NN
cord-276978-xl4u7n6r	144	15	using	use	VBG
cord-276978-xl4u7n6r	144	16	Phylogenetic	phylogenetic	JJ
cord-276978-xl4u7n6r	144	17	analysis	analysis	NN
cord-276978-xl4u7n6r	144	18	of	of	IN
cord-276978-xl4u7n6r	144	19	a	a	DT
cord-276978-xl4u7n6r	144	20	highly	highly	RB
cord-276978-xl4u7n6r	144	21	conserved	conserve	VBN
cord-276978-xl4u7n6r	144	22	region	region	NN
cord-276978-xl4u7n6r	144	23	of	of	IN
cord-276978-xl4u7n6r	144	24	the	the	DT
cord-276978-xl4u7n6r	144	25	polymerase	polymerase	NN
cord-276978-xl4u7n6r	144	26	gene	gene	NN
cord-276978-xl4u7n6r	144	27	from	from	IN
cord-276978-xl4u7n6r	144	28	11	11	CD
cord-276978-xl4u7n6r	144	29	coronaviruses	coronaviruse	NNS
cord-276978-xl4u7n6r	144	30	and	and	CC
cord-276978-xl4u7n6r	144	31	development	development	NN
cord-276978-xl4u7n6r	144	32	of	of	IN
cord-276978-xl4u7n6r	144	33	a	a	DT
cord-276978-xl4u7n6r	144	34	consensus	consensus	NN
cord-276978-xl4u7n6r	144	35	polymerase	polymerase	NN
cord-276978-xl4u7n6r	144	36	chain	chain	NN
cord-276978-xl4u7n6r	144	37	reaction	reaction	NN
cord-276978-xl4u7n6r	144	38	assay	assay	NN
cord-276978-xl4u7n6r	144	39	Virus	virus	NN
cord-276978-xl4u7n6r	144	40	Res	Res	NNP
cord-276978-xl4u7n6r	144	41	Complete	complete	JJ
cord-276978-xl4u7n6r	144	42	genomic	genomic	JJ
cord-276978-xl4u7n6r	144	43	sequences	sequence	NNS
cord-276978-xl4u7n6r	144	44	of	of	IN
cord-276978-xl4u7n6r	144	45	astroviruses	astroviruse	NNS
cord-276978-xl4u7n6r	144	46	from	from	IN
cord-276978-xl4u7n6r	144	47	sheep	sheep	NN
cord-276978-xl4u7n6r	144	48	and	and	CC
cord-276978-xl4u7n6r	144	49	turkey	turkey	NNP
cord-276978-xl4u7n6r	144	50	:	:	:
cord-276978-xl4u7n6r	144	51	comparison	comparison	NN
cord-276978-xl4u7n6r	144	52	with	with	IN
cord-276978-xl4u7n6r	144	53	related	related	JJ
cord-276978-xl4u7n6r	144	54	viruses	virus	NNS
cord-276978-xl4u7n6r	144	55	Virus	virus	NN
cord-276978-xl4u7n6r	144	56	Res	res	NN