id	sid	tid	token	lemma	pos
cord-265978-i0fu8e0p	1	1	key	key	JJ
cord-265978-i0fu8e0p	1	2	:	:	:
cord-265978-i0fu8e0p	1	3	cord-265978-i0fu8e0p	cord-265978-i0fu8e0p	NN
cord-265978-i0fu8e0p	1	4	authors	author	NNS
cord-265978-i0fu8e0p	1	5	:	:	:
cord-265978-i0fu8e0p	1	6	Amer	Amer	NNP
cord-265978-i0fu8e0p	1	7	,	,	,
cord-265978-i0fu8e0p	1	8	Haitham	Haitham	NNP
cord-265978-i0fu8e0p	1	9	M.	M.	NNP
cord-265978-i0fu8e0p	1	10	;	;	:
cord-265978-i0fu8e0p	1	11	Almajhdi	Almajhdi	NNP
cord-265978-i0fu8e0p	1	12	,	,	,
cord-265978-i0fu8e0p	1	13	Fahad	Fahad	NNP
cord-265978-i0fu8e0p	1	14	N.	N.	NNP
cord-265978-i0fu8e0p	1	15	title	title	NN
cord-265978-i0fu8e0p	1	16	:	:	:
cord-265978-i0fu8e0p	1	17	Development	development	NN
cord-265978-i0fu8e0p	1	18	of	of	IN
cord-265978-i0fu8e0p	1	19	a	a	DT
cord-265978-i0fu8e0p	1	20	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	1	21	Green	Green	NNP
cord-265978-i0fu8e0p	1	22	I	-PRON-	PRP
cord-265978-i0fu8e0p	1	23	based	base	VBD
cord-265978-i0fu8e0p	1	24	real	real	JJ
cord-265978-i0fu8e0p	1	25	-	-	HYPH
cord-265978-i0fu8e0p	1	26	time	time	NN
cord-265978-i0fu8e0p	1	27	RT	RT	NNP
cord-265978-i0fu8e0p	1	28	-	-	HYPH
cord-265978-i0fu8e0p	1	29	PCR	PCR	NNP
cord-265978-i0fu8e0p	1	30	assay	assay	NN
cord-265978-i0fu8e0p	1	31	for	for	IN
cord-265978-i0fu8e0p	1	32	detection	detection	NN
cord-265978-i0fu8e0p	1	33	and	and	CC
cord-265978-i0fu8e0p	1	34	quantification	quantification	NN
cord-265978-i0fu8e0p	1	35	of	of	IN
cord-265978-i0fu8e0p	1	36	bovine	bovine	JJ
cord-265978-i0fu8e0p	1	37	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	1	38	date	date	NN
cord-265978-i0fu8e0p	1	39	:	:	:
cord-265978-i0fu8e0p	1	40	2011	2011	CD
cord-265978-i0fu8e0p	1	41	-	-	SYM
cord-265978-i0fu8e0p	1	42	06	06	CD
cord-265978-i0fu8e0p	1	43	-	-	SYM
cord-265978-i0fu8e0p	1	44	30	30	CD
cord-265978-i0fu8e0p	1	45	journal	journal	NN
cord-265978-i0fu8e0p	1	46	:	:	:
cord-265978-i0fu8e0p	2	1	Molecular	molecular	JJ
cord-265978-i0fu8e0p	2	2	and	and	CC
cord-265978-i0fu8e0p	2	3	Cellular	Cellular	NNP
cord-265978-i0fu8e0p	2	4	Probes	Probes	NNPS
cord-265978-i0fu8e0p	2	5	DOI	DOI	NNP
cord-265978-i0fu8e0p	2	6	:	:	:
cord-265978-i0fu8e0p	2	7	10.1016	10.1016	CD
cord-265978-i0fu8e0p	2	8	/	/	SYM
cord-265978-i0fu8e0p	2	9	j.mcp.2011.03.001	j.mcp.2011.03.001	JJ
cord-265978-i0fu8e0p	2	10	sha	sha	NNP
cord-265978-i0fu8e0p	2	11	:	:	:
cord-265978-i0fu8e0p	3	1	75441498b30257e8d2ce87c2eb4daf445fd11a4f	75441498b30257e8d2ce87c2eb4daf445fd11a4f	CD
cord-265978-i0fu8e0p	3	2	doc_id	doc_id	CD
cord-265978-i0fu8e0p	4	1	:	:	:
cord-265978-i0fu8e0p	4	2	265978	265978	CD
cord-265978-i0fu8e0p	4	3	cord_uid	cord_uid	NNS
cord-265978-i0fu8e0p	4	4	:	:	:
cord-265978-i0fu8e0p	5	1	i0fu8e0p	i0fu8e0p	NNP
cord-265978-i0fu8e0p	6	1	Abstract	Abstract	NNP
cord-265978-i0fu8e0p	6	2	A	a	DT
cord-265978-i0fu8e0p	6	3	novel	novel	JJ
cord-265978-i0fu8e0p	6	4	two	two	CD
cord-265978-i0fu8e0p	6	5	-	-	HYPH
cord-265978-i0fu8e0p	6	6	step	step	NN
cord-265978-i0fu8e0p	6	7	,	,	,
cord-265978-i0fu8e0p	6	8	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	6	9	Green	Green	NNP
cord-265978-i0fu8e0p	6	10	I	-PRON-	PRP
cord-265978-i0fu8e0p	6	11	based	base	VBD
cord-265978-i0fu8e0p	6	12	real	real	JJ
cord-265978-i0fu8e0p	6	13	-	-	HYPH
cord-265978-i0fu8e0p	6	14	time	time	NN
cord-265978-i0fu8e0p	6	15	RT	RT	NNP
cord-265978-i0fu8e0p	6	16	-	-	HYPH
cord-265978-i0fu8e0p	6	17	PCR	PCR	NNP
cord-265978-i0fu8e0p	6	18	assay	assay	NN
cord-265978-i0fu8e0p	6	19	was	be	VBD
cord-265978-i0fu8e0p	6	20	developed	develop	VBN
cord-265978-i0fu8e0p	6	21	for	for	IN
cord-265978-i0fu8e0p	6	22	detection	detection	NN
cord-265978-i0fu8e0p	6	23	and	and	CC
cord-265978-i0fu8e0p	6	24	quantification	quantification	NN
cord-265978-i0fu8e0p	6	25	of	of	IN
cord-265978-i0fu8e0p	6	26	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	6	27	using	use	VBG
cord-265978-i0fu8e0p	6	28	ABI	ABI	NNP
cord-265978-i0fu8e0p	6	29	PRISM	PRISM	NNP
cord-265978-i0fu8e0p	6	30	7500	7500	CD
cord-265978-i0fu8e0p	6	31	sequence	sequence	NN
cord-265978-i0fu8e0p	6	32	detection	detection	NN
cord-265978-i0fu8e0p	6	33	system	system	NN
cord-265978-i0fu8e0p	6	34	.	.	.
cord-265978-i0fu8e0p	7	1	The	the	DT
cord-265978-i0fu8e0p	7	2	assay	assay	NN
cord-265978-i0fu8e0p	7	3	was	be	VBD
cord-265978-i0fu8e0p	7	4	carried	carry	VBN
cord-265978-i0fu8e0p	7	5	out	out	RP
cord-265978-i0fu8e0p	7	6	using	use	VBG
cord-265978-i0fu8e0p	7	7	two	two	CD
cord-265978-i0fu8e0p	7	8	sets	set	NNS
cord-265978-i0fu8e0p	7	9	of	of	IN
cord-265978-i0fu8e0p	7	10	primers	primer	NNS
cord-265978-i0fu8e0p	7	11	designed	design	VBN
cord-265978-i0fu8e0p	7	12	to	to	TO
cord-265978-i0fu8e0p	7	13	amplify	amplify	VB
cord-265978-i0fu8e0p	7	14	highly	highly	RB
cord-265978-i0fu8e0p	7	15	conserved	conserve	VBN
cord-265978-i0fu8e0p	7	16	sequences	sequence	NNS
cord-265978-i0fu8e0p	7	17	of	of	IN
cord-265978-i0fu8e0p	7	18	the	the	DT
cord-265978-i0fu8e0p	7	19	nucleocapsid	nucleocapsid	NNP
cord-265978-i0fu8e0p	7	20	gene	gene	NN
cord-265978-i0fu8e0p	7	21	of	of	IN
cord-265978-i0fu8e0p	7	22	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	7	23	and	and	CC
cord-265978-i0fu8e0p	7	24	the	the	DT
cord-265978-i0fu8e0p	7	25	internal	internal	JJ
cord-265978-i0fu8e0p	7	26	control	control	NN
cord-265978-i0fu8e0p	7	27	,	,	,
cord-265978-i0fu8e0p	7	28	bovine	bovine	JJ
cord-265978-i0fu8e0p	7	29	glyceraldehyde-3-phosphate	glyceraldehyde-3-phosphate	NNP
cord-265978-i0fu8e0p	7	30	dehydrogenase	dehydrogenase	NN
cord-265978-i0fu8e0p	7	31	,	,	,
cord-265978-i0fu8e0p	7	32	RNA	RNA	NNP
cord-265978-i0fu8e0p	7	33	.	.	.
cord-265978-i0fu8e0p	8	1	Specific	specific	JJ
cord-265978-i0fu8e0p	8	2	identification	identification	NN
cord-265978-i0fu8e0p	8	3	of	of	IN
cord-265978-i0fu8e0p	8	4	both	both	DT
cord-265978-i0fu8e0p	8	5	targets	target	NNS
cord-265978-i0fu8e0p	8	6	was	be	VBD
cord-265978-i0fu8e0p	8	7	elucidated	elucidate	VBN
cord-265978-i0fu8e0p	8	8	by	by	IN
cord-265978-i0fu8e0p	8	9	melt	melt	NN
cord-265978-i0fu8e0p	8	10	curve	curve	NN
cord-265978-i0fu8e0p	8	11	analysis	analysis	NN
cord-265978-i0fu8e0p	8	12	,	,	,
cord-265978-i0fu8e0p	8	13	in	in	IN
cord-265978-i0fu8e0p	8	14	which	which	WDT
cord-265978-i0fu8e0p	8	15	the	the	DT
cord-265978-i0fu8e0p	8	16	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	8	17	amplified	amplify	VBN
cord-265978-i0fu8e0p	8	18	product	product	NN
cord-265978-i0fu8e0p	8	19	generated	generate	VBD
cord-265978-i0fu8e0p	8	20	a	a	DT
cord-265978-i0fu8e0p	8	21	melt	melt	NN
cord-265978-i0fu8e0p	8	22	peak	peak	NN
cord-265978-i0fu8e0p	8	23	at	at	IN
cord-265978-i0fu8e0p	8	24	78.35	78.35	CD
cord-265978-i0fu8e0p	8	25	±	±	CD
cord-265978-i0fu8e0p	8	26	0.26	0.26	CD
cord-265978-i0fu8e0p	8	27	°	°	NN
cord-265978-i0fu8e0p	8	28	C	c	NN
cord-265978-i0fu8e0p	8	29	and	and	CC
cord-265978-i0fu8e0p	8	30	the	the	DT
cord-265978-i0fu8e0p	8	31	internal	internal	JJ
cord-265978-i0fu8e0p	8	32	control	control	NN
cord-265978-i0fu8e0p	8	33	RNA	RNA	NNP
cord-265978-i0fu8e0p	8	34	at	at	IN
cord-265978-i0fu8e0p	8	35	82.54	82.54	CD
cord-265978-i0fu8e0p	8	36	±	±	CD
cord-265978-i0fu8e0p	8	37	0.32	0.32	CD
cord-265978-i0fu8e0p	8	38	°	°	NN
cord-265978-i0fu8e0p	8	39	C	c	NN
cord-265978-i0fu8e0p	8	40	.	.	.
cord-265978-i0fu8e0p	9	1	The	the	DT
cord-265978-i0fu8e0p	9	2	assay	assay	NN
cord-265978-i0fu8e0p	9	3	was	be	VBD
cord-265978-i0fu8e0p	9	4	highly	highly	RB
cord-265978-i0fu8e0p	9	5	specific	specific	JJ
cord-265978-i0fu8e0p	9	6	since	since	IN
cord-265978-i0fu8e0p	9	7	all	all	DT
cord-265978-i0fu8e0p	9	8	negative	negative	JJ
cord-265978-i0fu8e0p	9	9	controls	control	NNS
cord-265978-i0fu8e0p	9	10	and	and	CC
cord-265978-i0fu8e0p	9	11	other	other	JJ
cord-265978-i0fu8e0p	9	12	viruses	virus	NNS
cord-265978-i0fu8e0p	9	13	of	of	IN
cord-265978-i0fu8e0p	9	14	clinical	clinical	JJ
cord-265978-i0fu8e0p	9	15	and	and	CC
cord-265978-i0fu8e0p	9	16	structural	structural	JJ
cord-265978-i0fu8e0p	9	17	relevance	relevance	NN
cord-265978-i0fu8e0p	9	18	failed	fail	VBD
cord-265978-i0fu8e0p	9	19	to	to	TO
cord-265978-i0fu8e0p	9	20	develop	develop	VB
cord-265978-i0fu8e0p	9	21	any	any	DT
cord-265978-i0fu8e0p	9	22	positive	positive	JJ
cord-265978-i0fu8e0p	9	23	results	result	NNS
cord-265978-i0fu8e0p	9	24	.	.	.
cord-265978-i0fu8e0p	10	1	The	the	DT
cord-265978-i0fu8e0p	10	2	detection	detection	NN
cord-265978-i0fu8e0p	10	3	limit	limit	NN
cord-265978-i0fu8e0p	10	4	of	of	IN
cord-265978-i0fu8e0p	10	5	the	the	DT
cord-265978-i0fu8e0p	10	6	reaction	reaction	NN
cord-265978-i0fu8e0p	10	7	was	be	VBD
cord-265978-i0fu8e0p	10	8	103	103	CD
cord-265978-i0fu8e0p	10	9	plasmid	plasmid	NN
cord-265978-i0fu8e0p	10	10	copies	copy	NNS
cord-265978-i0fu8e0p	10	11	and	and	CC
cord-265978-i0fu8e0p	10	12	1.17	1.17	CD
cord-265978-i0fu8e0p	10	13	×	×	NN
cord-265978-i0fu8e0p	11	1	10−3	10−3	CD
cord-265978-i0fu8e0p	11	2	TCID50	tcid50	CD
cord-265978-i0fu8e0p	11	3	of	of	IN
cord-265978-i0fu8e0p	11	4	the	the	DT
cord-265978-i0fu8e0p	11	5	tissue	tissue	NN
cord-265978-i0fu8e0p	11	6	culture	culture	NN
cord-265978-i0fu8e0p	11	7	propagated	propagate	VBD
cord-265978-i0fu8e0p	11	8	virus	virus	NN
cord-265978-i0fu8e0p	11	9	.	.	.
cord-265978-i0fu8e0p	12	1	Standard	standard	JJ
cord-265978-i0fu8e0p	12	2	deviation	deviation	NN
cord-265978-i0fu8e0p	12	3	and	and	CC
cord-265978-i0fu8e0p	12	4	coefficient	coefficient	NN
cord-265978-i0fu8e0p	12	5	of	of	IN
cord-265978-i0fu8e0p	12	6	variation	variation	NN
cord-265978-i0fu8e0p	12	7	was	be	VBD
cord-265978-i0fu8e0p	12	8	low	low	JJ
cord-265978-i0fu8e0p	12	9	for	for	IN
cord-265978-i0fu8e0p	12	10	both	both	DT
cord-265978-i0fu8e0p	12	11	intra	intra	JJ
cord-265978-i0fu8e0p	12	12	-	-	NN
cord-265978-i0fu8e0p	12	13	assay	assay	JJ
cord-265978-i0fu8e0p	12	14	and	and	CC
cord-265978-i0fu8e0p	12	15	inter	inter	JJ
cord-265978-i0fu8e0p	12	16	-	-	JJ
cord-265978-i0fu8e0p	12	17	assay	assay	JJ
cord-265978-i0fu8e0p	12	18	variability	variability	NN
cord-265978-i0fu8e0p	12	19	.	.	.
cord-265978-i0fu8e0p	13	1	The	the	DT
cord-265978-i0fu8e0p	13	2	assay	assay	NN
cord-265978-i0fu8e0p	13	3	performance	performance	NN
cord-265978-i0fu8e0p	13	4	on	on	IN
cord-265978-i0fu8e0p	13	5	field	field	NN
cord-265978-i0fu8e0p	13	6	samples	sample	NNS
cord-265978-i0fu8e0p	13	7	was	be	VBD
cord-265978-i0fu8e0p	13	8	evaluated	evaluate	VBN
cord-265978-i0fu8e0p	13	9	on	on	IN
cord-265978-i0fu8e0p	13	10	103	103	CD
cord-265978-i0fu8e0p	13	11	(	(	-LRB-
cord-265978-i0fu8e0p	13	12	68	68	CD
cord-265978-i0fu8e0p	13	13	fecal	fecal	JJ
cord-265978-i0fu8e0p	13	14	and	and	CC
cord-265978-i0fu8e0p	13	15	35	35	CD
cord-265978-i0fu8e0p	13	16	nasal	nasal	NN
cord-265978-i0fu8e0p	13	17	)	)	-RRB-
cord-265978-i0fu8e0p	13	18	swab	swab	NN
cord-265978-i0fu8e0p	13	19	specimens	specimen	NNS
cord-265978-i0fu8e0p	13	20	and	and	CC
cord-265978-i0fu8e0p	13	21	compared	compare	VBN
cord-265978-i0fu8e0p	13	22	with	with	IN
cord-265978-i0fu8e0p	13	23	the	the	DT
cord-265978-i0fu8e0p	13	24	conventional	conventional	JJ
cord-265978-i0fu8e0p	13	25	RT	RT	NNP
cord-265978-i0fu8e0p	13	26	-	-	HYPH
cord-265978-i0fu8e0p	13	27	PCR	PCR	NNP
cord-265978-i0fu8e0p	13	28	assay	assay	NN
cord-265978-i0fu8e0p	13	29	.	.	.
cord-265978-i0fu8e0p	14	1	The	the	DT
cord-265978-i0fu8e0p	14	2	results	result	NNS
cord-265978-i0fu8e0p	14	3	of	of	IN
cord-265978-i0fu8e0p	14	4	both	both	DT
cord-265978-i0fu8e0p	14	5	assays	assay	NNS
cord-265978-i0fu8e0p	14	6	matched	match	VBN
cord-265978-i0fu8e0p	14	7	for	for	IN
cord-265978-i0fu8e0p	14	8	the	the	DT
cord-265978-i0fu8e0p	14	9	diagnosis	diagnosis	NN
cord-265978-i0fu8e0p	14	10	of	of	IN
cord-265978-i0fu8e0p	14	11	65	65	CD
cord-265978-i0fu8e0p	14	12	fecal	fecal	NN
cord-265978-i0fu8e0p	14	13	and	and	CC
cord-265978-i0fu8e0p	14	14	33	33	CD
cord-265978-i0fu8e0p	14	15	nasal	nasal	NN
cord-265978-i0fu8e0p	14	16	samples	sample	NNS
cord-265978-i0fu8e0p	14	17	.	.	.
cord-265978-i0fu8e0p	15	1	However	however	RB
cord-265978-i0fu8e0p	15	2	,	,	,
cord-265978-i0fu8e0p	15	3	three	three	CD
cord-265978-i0fu8e0p	15	4	fecal	fecal	JJ
cord-265978-i0fu8e0p	15	5	and	and	CC
cord-265978-i0fu8e0p	15	6	two	two	CD
cord-265978-i0fu8e0p	15	7	nasal	nasal	NN
cord-265978-i0fu8e0p	15	8	samples	sample	NNS
cord-265978-i0fu8e0p	15	9	tested	test	VBD
cord-265978-i0fu8e0p	15	10	negative	negative	JJ
cord-265978-i0fu8e0p	15	11	in	in	IN
cord-265978-i0fu8e0p	15	12	gel	gel	NN
cord-265978-i0fu8e0p	15	13	-	-	HYPH
cord-265978-i0fu8e0p	15	14	based	base	VBN
cord-265978-i0fu8e0p	15	15	assay	assay	NN
cord-265978-i0fu8e0p	15	16	were	be	VBD
cord-265978-i0fu8e0p	15	17	positive	positive	JJ
cord-265978-i0fu8e0p	15	18	for	for	IN
cord-265978-i0fu8e0p	15	19	the	the	DT
cord-265978-i0fu8e0p	15	20	real	real	JJ
cord-265978-i0fu8e0p	15	21	-	-	HYPH
cord-265978-i0fu8e0p	15	22	time	time	NN
cord-265978-i0fu8e0p	15	23	RT	RT	NNP
cord-265978-i0fu8e0p	15	24	-	-	HYPH
cord-265978-i0fu8e0p	15	25	PCR	PCR	NNP
cord-265978-i0fu8e0p	15	26	.	.	.
cord-265978-i0fu8e0p	16	1	The	the	DT
cord-265978-i0fu8e0p	16	2	robustness	robustness	NN
cord-265978-i0fu8e0p	16	3	and	and	CC
cord-265978-i0fu8e0p	16	4	a	a	DT
cord-265978-i0fu8e0p	16	5	high	high	JJ
cord-265978-i0fu8e0p	16	6	-	-	HYPH
cord-265978-i0fu8e0p	16	7	throughput	throughput	NN
cord-265978-i0fu8e0p	16	8	performance	performance	NN
cord-265978-i0fu8e0p	16	9	of	of	IN
cord-265978-i0fu8e0p	16	10	the	the	DT
cord-265978-i0fu8e0p	16	11	developed	developed	JJ
cord-265978-i0fu8e0p	16	12	assay	assay	NN
cord-265978-i0fu8e0p	16	13	make	make	VB
cord-265978-i0fu8e0p	16	14	it	-PRON-	PRP
cord-265978-i0fu8e0p	16	15	a	a	DT
cord-265978-i0fu8e0p	16	16	powerful	powerful	JJ
cord-265978-i0fu8e0p	16	17	tool	tool	NN
cord-265978-i0fu8e0p	16	18	in	in	IN
cord-265978-i0fu8e0p	16	19	diagnostic	diagnostic	JJ
cord-265978-i0fu8e0p	16	20	applications	application	NNS
cord-265978-i0fu8e0p	16	21	and	and	CC
cord-265978-i0fu8e0p	16	22	in	in	IN
cord-265978-i0fu8e0p	16	23	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	16	24	research	research	NN
cord-265978-i0fu8e0p	16	25	.	.	.
cord-265978-i0fu8e0p	17	1	Bovine	bovine	JJ
cord-265978-i0fu8e0p	17	2	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	17	3	is	be	VBZ
cord-265978-i0fu8e0p	17	4	the	the	DT
cord-265978-i0fu8e0p	17	5	second	second	JJ
cord-265978-i0fu8e0p	17	6	major	major	JJ
cord-265978-i0fu8e0p	17	7	cause	cause	NN
cord-265978-i0fu8e0p	17	8	of	of	IN
cord-265978-i0fu8e0p	17	9	diarrhea	diarrhea	NN
cord-265978-i0fu8e0p	17	10	that	that	WDT
cord-265978-i0fu8e0p	17	11	affects	affect	VBZ
cord-265978-i0fu8e0p	17	12	calves	calf	NNS
cord-265978-i0fu8e0p	17	13	during	during	IN
cord-265978-i0fu8e0p	17	14	the	the	DT
cord-265978-i0fu8e0p	17	15	first	first	JJ
cord-265978-i0fu8e0p	17	16	3	3	CD
cord-265978-i0fu8e0p	17	17	weeks	week	NNS
cord-265978-i0fu8e0p	17	18	of	of	IN
cord-265978-i0fu8e0p	17	19	life	life	NN
cord-265978-i0fu8e0p	17	20	[	[	-LRB-
cord-265978-i0fu8e0p	17	21	1	1	CD
cord-265978-i0fu8e0p	17	22	]	]	-RRB-
cord-265978-i0fu8e0p	17	23	.	.	.
cord-265978-i0fu8e0p	18	1	The	the	DT
cord-265978-i0fu8e0p	18	2	virus	virus	NN
cord-265978-i0fu8e0p	18	3	also	also	RB
cord-265978-i0fu8e0p	18	4	infects	infect	VBZ
cord-265978-i0fu8e0p	18	5	the	the	DT
cord-265978-i0fu8e0p	18	6	bovine	bovine	JJ
cord-265978-i0fu8e0p	18	7	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	18	8	tract	tract	NN
cord-265978-i0fu8e0p	18	9	and	and	CC
cord-265978-i0fu8e0p	18	10	it	-PRON-	PRP
cord-265978-i0fu8e0p	18	11	has	have	VBZ
cord-265978-i0fu8e0p	18	12	been	be	VBN
cord-265978-i0fu8e0p	18	13	associated	associate	VBN
cord-265978-i0fu8e0p	18	14	with	with	IN
cord-265978-i0fu8e0p	18	15	winter	winter	NN
cord-265978-i0fu8e0p	18	16	dysentery	dysentery	NN
cord-265978-i0fu8e0p	18	17	in	in	IN
cord-265978-i0fu8e0p	18	18	adult	adult	NN
cord-265978-i0fu8e0p	18	19	cattle	cattle	NNS
cord-265978-i0fu8e0p	18	20	[	[	-LRB-
cord-265978-i0fu8e0p	18	21	2	2	CD
cord-265978-i0fu8e0p	18	22	,	,	,
cord-265978-i0fu8e0p	18	23	3	3	CD
cord-265978-i0fu8e0p	18	24	]	]	-RRB-
cord-265978-i0fu8e0p	18	25	.	.	.
cord-265978-i0fu8e0p	19	1	Disease	Disease	NNP
cord-265978-i0fu8e0p	19	2	outcomes	outcome	NNS
cord-265978-i0fu8e0p	19	3	'	'	POS
cord-265978-i0fu8e0p	19	4	induced	induce	VBN
cord-265978-i0fu8e0p	19	5	by	by	IN
cord-265978-i0fu8e0p	19	6	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	19	7	are	be	VBP
cord-265978-i0fu8e0p	19	8	responsible	responsible	JJ
cord-265978-i0fu8e0p	19	9	for	for	IN
cord-265978-i0fu8e0p	19	10	significant	significant	JJ
cord-265978-i0fu8e0p	19	11	economical	economical	JJ
cord-265978-i0fu8e0p	19	12	losses	loss	NNS
cord-265978-i0fu8e0p	19	13	to	to	TO
cord-265978-i0fu8e0p	19	14	beef	beef	NN
cord-265978-i0fu8e0p	19	15	and	and	CC
cord-265978-i0fu8e0p	19	16	dairy	dairy	NN
cord-265978-i0fu8e0p	19	17	industries	industry	NNS
cord-265978-i0fu8e0p	19	18	worldwide	worldwide	RB
cord-265978-i0fu8e0p	19	19	.	.	.
cord-265978-i0fu8e0p	20	1	These	these	DT
cord-265978-i0fu8e0p	20	2	losses	loss	NNS
cord-265978-i0fu8e0p	20	3	are	be	VBP
cord-265978-i0fu8e0p	20	4	not	not	RB
cord-265978-i0fu8e0p	20	5	only	only	RB
cord-265978-i0fu8e0p	20	6	the	the	DT
cord-265978-i0fu8e0p	20	7	figure	figure	NN
cord-265978-i0fu8e0p	20	8	of	of	IN
cord-265978-i0fu8e0p	20	9	mortalities	mortality	NNS
cord-265978-i0fu8e0p	20	10	,	,	,
cord-265978-i0fu8e0p	20	11	which	which	WDT
cord-265978-i0fu8e0p	20	12	may	may	MD
cord-265978-i0fu8e0p	20	13	reach	reach	VB
cord-265978-i0fu8e0p	20	14	80	80	CD
cord-265978-i0fu8e0p	20	15	%	%	NN
cord-265978-i0fu8e0p	20	16	in	in	IN
cord-265978-i0fu8e0p	20	17	some	some	DT
cord-265978-i0fu8e0p	20	18	complicated	complicated	JJ
cord-265978-i0fu8e0p	20	19	cases	case	NNS
cord-265978-i0fu8e0p	20	20	,	,	,
cord-265978-i0fu8e0p	20	21	but	but	CC
cord-265978-i0fu8e0p	20	22	also	also	RB
cord-265978-i0fu8e0p	20	23	reflect	reflect	VBP
cord-265978-i0fu8e0p	20	24	the	the	DT
cord-265978-i0fu8e0p	20	25	veterinary	veterinary	JJ
cord-265978-i0fu8e0p	20	26	costs	cost	NNS
cord-265978-i0fu8e0p	20	27	,	,	,
cord-265978-i0fu8e0p	20	28	medications	medication	NNS
cord-265978-i0fu8e0p	20	29	and	and	CC
cord-265978-i0fu8e0p	20	30	poor	poor	JJ
cord-265978-i0fu8e0p	20	31	productivity	productivity	NN
cord-265978-i0fu8e0p	20	32	of	of	IN
cord-265978-i0fu8e0p	20	33	affected	affect	VBN
cord-265978-i0fu8e0p	20	34	animals	animal	NNS
cord-265978-i0fu8e0p	20	35	[	[	-LRB-
cord-265978-i0fu8e0p	20	36	4	4	CD
cord-265978-i0fu8e0p	20	37	]	]	-RRB-
cord-265978-i0fu8e0p	20	38	.	.	.
cord-265978-i0fu8e0p	21	1	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	21	2	is	be	VBZ
cord-265978-i0fu8e0p	21	3	a	a	DT
cord-265978-i0fu8e0p	21	4	member	member	NN
cord-265978-i0fu8e0p	21	5	of	of	IN
cord-265978-i0fu8e0p	21	6	the	the	DT
cord-265978-i0fu8e0p	21	7	antigenic	antigenic	JJ
cord-265978-i0fu8e0p	21	8	group	group	NN
cord-265978-i0fu8e0p	21	9	2	2	CD
cord-265978-i0fu8e0p	21	10	of	of	IN
cord-265978-i0fu8e0p	21	11	genus	genus	NN
cord-265978-i0fu8e0p	21	12	Coronavirus	Coronavirus	NNP
cord-265978-i0fu8e0p	21	13	that	that	WDT
cord-265978-i0fu8e0p	21	14	belongs	belong	VBZ
cord-265978-i0fu8e0p	21	15	to	to	IN
cord-265978-i0fu8e0p	21	16	family	family	NN
cord-265978-i0fu8e0p	21	17	Coronaviridae	Coronaviridae	NNP
cord-265978-i0fu8e0p	21	18	within	within	IN
cord-265978-i0fu8e0p	21	19	the	the	DT
cord-265978-i0fu8e0p	21	20	order	order	NN
cord-265978-i0fu8e0p	22	1	Nidovirales	Nidovirales	NNP
cord-265978-i0fu8e0p	22	2	[	[	-LRB-
cord-265978-i0fu8e0p	22	3	5	5	CD
cord-265978-i0fu8e0p	22	4	]	]	-RRB-
cord-265978-i0fu8e0p	22	5	.	.	.
cord-265978-i0fu8e0p	23	1	The	the	DT
cord-265978-i0fu8e0p	23	2	virion	virion	NN
cord-265978-i0fu8e0p	23	3	possesses	possess	VBZ
cord-265978-i0fu8e0p	23	4	a	a	DT
cord-265978-i0fu8e0p	23	5	single	single	JJ
cord-265978-i0fu8e0p	23	6	stranded	strand	VBN
cord-265978-i0fu8e0p	23	7	,	,	,
cord-265978-i0fu8e0p	23	8	non	non	JJ
cord-265978-i0fu8e0p	23	9	-	-	JJ
cord-265978-i0fu8e0p	23	10	segmented	segmented	JJ
cord-265978-i0fu8e0p	23	11	,	,	,
cord-265978-i0fu8e0p	23	12	linear	linear	NNP
cord-265978-i0fu8e0p	23	13	RNA	RNA	NNP
cord-265978-i0fu8e0p	23	14	genome	genome	NN
cord-265978-i0fu8e0p	23	15	of	of	IN
cord-265978-i0fu8e0p	23	16	positive	positive	JJ
cord-265978-i0fu8e0p	23	17	polarity	polarity	NN
cord-265978-i0fu8e0p	23	18	that	that	WDT
cord-265978-i0fu8e0p	23	19	is	be	VBZ
cord-265978-i0fu8e0p	23	20	capped	cap	VBN
cord-265978-i0fu8e0p	23	21	,	,	,
cord-265978-i0fu8e0p	23	22	polyadenylated	polyadenylated	JJ
cord-265978-i0fu8e0p	23	23	and	and	CC
cord-265978-i0fu8e0p	23	24	infectious	infectious	JJ
cord-265978-i0fu8e0p	23	25	[	[	-LRB-
cord-265978-i0fu8e0p	23	26	6	6	CD
cord-265978-i0fu8e0p	23	27	]	]	-RRB-
cord-265978-i0fu8e0p	23	28	.	.	.
cord-265978-i0fu8e0p	24	1	The	the	DT
cord-265978-i0fu8e0p	24	2	viral	viral	JJ
cord-265978-i0fu8e0p	24	3	genome	genome	NN
cord-265978-i0fu8e0p	24	4	is	be	VBZ
cord-265978-i0fu8e0p	24	5	associated	associate	VBN
cord-265978-i0fu8e0p	24	6	with	with	IN
cord-265978-i0fu8e0p	24	7	the	the	DT
cord-265978-i0fu8e0p	24	8	nucleoprotein	nucleoprotein	NNP
cord-265978-i0fu8e0p	24	9	(	(	-LRB-
cord-265978-i0fu8e0p	24	10	N	N	NNP
cord-265978-i0fu8e0p	24	11	)	)	-RRB-
cord-265978-i0fu8e0p	24	12	to	to	TO
cord-265978-i0fu8e0p	24	13	form	form	VB
cord-265978-i0fu8e0p	24	14	a	a	DT
cord-265978-i0fu8e0p	24	15	long	long	JJ
cord-265978-i0fu8e0p	24	16	,	,	,
cord-265978-i0fu8e0p	24	17	flexible	flexible	JJ
cord-265978-i0fu8e0p	24	18	and	and	CC
cord-265978-i0fu8e0p	24	19	helical	helical	JJ
cord-265978-i0fu8e0p	24	20	nucleocapsid	nucleocapsid	NN
cord-265978-i0fu8e0p	24	21	.	.	.
cord-265978-i0fu8e0p	25	1	The	the	DT
cord-265978-i0fu8e0p	25	2	nucleocapsid	nucleocapsid	NN
cord-265978-i0fu8e0p	25	3	is	be	VBZ
cord-265978-i0fu8e0p	25	4	wrapped	wrap	VBN
cord-265978-i0fu8e0p	25	5	within	within	IN
cord-265978-i0fu8e0p	25	6	a	a	DT
cord-265978-i0fu8e0p	25	7	lipoprotein	lipoprotein	NN
cord-265978-i0fu8e0p	25	8	envelope	envelope	NN
cord-265978-i0fu8e0p	25	9	that	that	WDT
cord-265978-i0fu8e0p	25	10	carries	carry	VBZ
cord-265978-i0fu8e0p	25	11	four	four	CD
cord-265978-i0fu8e0p	25	12	structural	structural	JJ
cord-265978-i0fu8e0p	25	13	proteins	protein	NNS
cord-265978-i0fu8e0p	25	14	;	;	:
cord-265978-i0fu8e0p	25	15	the	the	DT
cord-265978-i0fu8e0p	25	16	integral	integral	JJ
cord-265978-i0fu8e0p	25	17	membrane	membrane	NN
cord-265978-i0fu8e0p	25	18	glycoprotein	glycoprotein	NN
cord-265978-i0fu8e0p	25	19	(	(	-LRB-
cord-265978-i0fu8e0p	25	20	M	M	NNP
cord-265978-i0fu8e0p	25	21	)	)	-RRB-
cord-265978-i0fu8e0p	25	22	;	;	:
cord-265978-i0fu8e0p	25	23	the	the	DT
cord-265978-i0fu8e0p	25	24	spike	spike	NN
cord-265978-i0fu8e0p	25	25	glycoprotein	glycoprotein	NN
cord-265978-i0fu8e0p	25	26	(	(	-LRB-
cord-265978-i0fu8e0p	25	27	S	s	NN
cord-265978-i0fu8e0p	25	28	)	)	-RRB-
cord-265978-i0fu8e0p	25	29	;	;	:
cord-265978-i0fu8e0p	25	30	the	the	DT
cord-265978-i0fu8e0p	25	31	hemagglutinineesterase	hemagglutinineesterase	NN
cord-265978-i0fu8e0p	25	32	glycoprotein	glycoprotein	NN
cord-265978-i0fu8e0p	25	33	(	(	-LRB-
cord-265978-i0fu8e0p	25	34	HE	HE	NNP
cord-265978-i0fu8e0p	25	35	)	)	-RRB-
cord-265978-i0fu8e0p	25	36	and	and	CC
cord-265978-i0fu8e0p	25	37	the	the	DT
cord-265978-i0fu8e0p	25	38	small	small	JJ
cord-265978-i0fu8e0p	25	39	envelope	envelope	NN
cord-265978-i0fu8e0p	25	40	protein	protein	NN
cord-265978-i0fu8e0p	25	41	(	(	-LRB-
cord-265978-i0fu8e0p	25	42	E	e	NN
cord-265978-i0fu8e0p	25	43	)	)	-RRB-
cord-265978-i0fu8e0p	25	44	[	[	-LRB-
cord-265978-i0fu8e0p	25	45	7	7	CD
cord-265978-i0fu8e0p	25	46	]	]	-RRB-
cord-265978-i0fu8e0p	25	47	.	.	.
cord-265978-i0fu8e0p	26	1	Although	although	IN
cord-265978-i0fu8e0p	26	2	several	several	JJ
cord-265978-i0fu8e0p	26	3	methods	method	NNS
cord-265978-i0fu8e0p	26	4	have	have	VBP
cord-265978-i0fu8e0p	26	5	been	be	VBN
cord-265978-i0fu8e0p	26	6	used	use	VBN
cord-265978-i0fu8e0p	26	7	for	for	IN
cord-265978-i0fu8e0p	26	8	diagnosis	diagnosis	NN
cord-265978-i0fu8e0p	26	9	of	of	IN
cord-265978-i0fu8e0p	26	10	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	26	11	infections	infection	NNS
cord-265978-i0fu8e0p	26	12	,	,	,
cord-265978-i0fu8e0p	26	13	there	there	EX
cord-265978-i0fu8e0p	26	14	is	be	VBZ
cord-265978-i0fu8e0p	26	15	no	no	DT
cord-265978-i0fu8e0p	26	16	"	"	``
cord-265978-i0fu8e0p	26	17	gold	gold	JJ
cord-265978-i0fu8e0p	26	18	standard	standard	NN
cord-265978-i0fu8e0p	26	19	"	"	''
cord-265978-i0fu8e0p	26	20	test	test	NN
cord-265978-i0fu8e0p	26	21	exists	exist	VBZ
cord-265978-i0fu8e0p	26	22	.	.	.
cord-265978-i0fu8e0p	27	1	Virus	virus	NN
cord-265978-i0fu8e0p	27	2	isolation	isolation	NN
cord-265978-i0fu8e0p	27	3	with	with	IN
cord-265978-i0fu8e0p	27	4	the	the	DT
cord-265978-i0fu8e0p	27	5	G	g	NN
cord-265978-i0fu8e0p	27	6	clone	clone	NN
cord-265978-i0fu8e0p	27	7	of	of	IN
cord-265978-i0fu8e0p	27	8	HRT-18	HRT-18	NNP
cord-265978-i0fu8e0p	27	9	cells	cell	NNS
cord-265978-i0fu8e0p	27	10	has	have	VBZ
cord-265978-i0fu8e0p	27	11	long	long	RB
cord-265978-i0fu8e0p	27	12	been	be	VBN
cord-265978-i0fu8e0p	27	13	considered	consider	VBN
cord-265978-i0fu8e0p	27	14	the	the	DT
cord-265978-i0fu8e0p	27	15	most	most	RBS
cord-265978-i0fu8e0p	27	16	sensitive	sensitive	JJ
cord-265978-i0fu8e0p	27	17	tool	tool	NN
cord-265978-i0fu8e0p	27	18	available	available	JJ
cord-265978-i0fu8e0p	27	19	but	but	CC
cord-265978-i0fu8e0p	27	20	this	this	DT
cord-265978-i0fu8e0p	27	21	procedure	procedure	NN
cord-265978-i0fu8e0p	27	22	was	be	VBD
cord-265978-i0fu8e0p	27	23	difficult	difficult	JJ
cord-265978-i0fu8e0p	27	24	,	,	,
cord-265978-i0fu8e0p	27	25	timeconsuming	timeconsuming	JJ
cord-265978-i0fu8e0p	27	26	and	and	CC
cord-265978-i0fu8e0p	27	27	not	not	RB
cord-265978-i0fu8e0p	27	28	practical	practical	JJ
cord-265978-i0fu8e0p	27	29	for	for	IN
cord-265978-i0fu8e0p	27	30	high	high	JJ
cord-265978-i0fu8e0p	27	31	-	-	HYPH
cord-265978-i0fu8e0p	27	32	throughput	throughput	NN
cord-265978-i0fu8e0p	27	33	screening	screening	NN
cord-265978-i0fu8e0p	27	34	of	of	IN
cord-265978-i0fu8e0p	27	35	samples	sample	NNS
cord-265978-i0fu8e0p	27	36	[	[	-LRB-
cord-265978-i0fu8e0p	27	37	8	8	CD
cord-265978-i0fu8e0p	27	38	]	]	-RRB-
cord-265978-i0fu8e0p	27	39	.	.	.
cord-265978-i0fu8e0p	28	1	Molecular	molecular	JJ
cord-265978-i0fu8e0p	28	2	assays	assay	NNS
cord-265978-i0fu8e0p	28	3	such	such	JJ
cord-265978-i0fu8e0p	28	4	as	as	IN
cord-265978-i0fu8e0p	28	5	RT	RT	NNP
cord-265978-i0fu8e0p	28	6	-	-	HYPH
cord-265978-i0fu8e0p	28	7	PCR	PCR	NNP
cord-265978-i0fu8e0p	28	8	offered	offer	VBD
cord-265978-i0fu8e0p	28	9	a	a	DT
cord-265978-i0fu8e0p	28	10	suitable	suitable	JJ
cord-265978-i0fu8e0p	28	11	alternative	alternative	NN
cord-265978-i0fu8e0p	28	12	to	to	IN
cord-265978-i0fu8e0p	28	13	virus	virus	NN
cord-265978-i0fu8e0p	28	14	isolation	isolation	NN
cord-265978-i0fu8e0p	28	15	since	since	IN
cord-265978-i0fu8e0p	28	16	they	-PRON-	PRP
cord-265978-i0fu8e0p	28	17	are	be	VBP
cord-265978-i0fu8e0p	28	18	faster	fast	JJR
cord-265978-i0fu8e0p	28	19	,	,	,
cord-265978-i0fu8e0p	28	20	simpler	simple	JJR
cord-265978-i0fu8e0p	28	21	yet	yet	CC
cord-265978-i0fu8e0p	28	22	maintain	maintain	VB
cord-265978-i0fu8e0p	28	23	the	the	DT
cord-265978-i0fu8e0p	28	24	higher	high	JJR
cord-265978-i0fu8e0p	28	25	performance	performance	NN
cord-265978-i0fu8e0p	28	26	and	and	CC
cord-265978-i0fu8e0p	28	27	accuracy	accuracy	NN
cord-265978-i0fu8e0p	28	28	required	require	VBN
cord-265978-i0fu8e0p	28	29	for	for	IN
cord-265978-i0fu8e0p	28	30	conclusive	conclusive	JJ
cord-265978-i0fu8e0p	28	31	rapid	rapid	JJ
cord-265978-i0fu8e0p	28	32	diagnosis	diagnosis	NN
cord-265978-i0fu8e0p	28	33	of	of	IN
cord-265978-i0fu8e0p	28	34	field	field	NN
cord-265978-i0fu8e0p	28	35	outbreaks	outbreak	NNS
cord-265978-i0fu8e0p	28	36	[	[	-LRB-
cord-265978-i0fu8e0p	28	37	9	9	CD
cord-265978-i0fu8e0p	28	38	,	,	,
cord-265978-i0fu8e0p	28	39	10	10	CD
cord-265978-i0fu8e0p	28	40	]	]	-RRB-
cord-265978-i0fu8e0p	28	41	.	.	.
cord-265978-i0fu8e0p	29	1	The	the	DT
cord-265978-i0fu8e0p	29	2	advent	advent	NN
cord-265978-i0fu8e0p	29	3	of	of	IN
cord-265978-i0fu8e0p	29	4	real	real	JJ
cord-265978-i0fu8e0p	29	5	-	-	HYPH
cord-265978-i0fu8e0p	29	6	time	time	NN
cord-265978-i0fu8e0p	29	7	PCR	PCR	NNP
cord-265978-i0fu8e0p	29	8	methods	method	NNS
cord-265978-i0fu8e0p	29	9	has	have	VBZ
cord-265978-i0fu8e0p	29	10	further	further	RB
cord-265978-i0fu8e0p	29	11	improved	improve	VBN
cord-265978-i0fu8e0p	29	12	the	the	DT
cord-265978-i0fu8e0p	29	13	significant	significant	JJ
cord-265978-i0fu8e0p	29	14	benefits	benefit	NNS
cord-265978-i0fu8e0p	29	15	of	of	IN
cord-265978-i0fu8e0p	29	16	RT	RT	NNP
cord-265978-i0fu8e0p	29	17	-	-	HYPH
cord-265978-i0fu8e0p	29	18	PCR	PCR	NNP
cord-265978-i0fu8e0p	29	19	.	.	.
cord-265978-i0fu8e0p	30	1	Compared	compare	VBN
cord-265978-i0fu8e0p	30	2	to	to	IN
cord-265978-i0fu8e0p	30	3	conventional	conventional	JJ
cord-265978-i0fu8e0p	30	4	gel	gel	NN
cord-265978-i0fu8e0p	30	5	-	-	HYPH
cord-265978-i0fu8e0p	30	6	based	base	VBN
cord-265978-i0fu8e0p	30	7	PCR	PCR	NNP
cord-265978-i0fu8e0p	30	8	assays	assay	NNS
cord-265978-i0fu8e0p	30	9	,	,	,
cord-265978-i0fu8e0p	30	10	real	real	JJ
cord-265978-i0fu8e0p	30	11	-	-	HYPH
cord-265978-i0fu8e0p	30	12	time	time	NN
cord-265978-i0fu8e0p	30	13	PCR	PCR	NNP
cord-265978-i0fu8e0p	30	14	has	have	VBZ
cord-265978-i0fu8e0p	30	15	many	many	JJ
cord-265978-i0fu8e0p	30	16	advantages	advantage	NNS
cord-265978-i0fu8e0p	30	17	including	include	VBG
cord-265978-i0fu8e0p	30	18	rapidity	rapidity	NN
cord-265978-i0fu8e0p	30	19	,	,	,
cord-265978-i0fu8e0p	30	20	higher	high	JJR
cord-265978-i0fu8e0p	30	21	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	30	22	,	,	,
cord-265978-i0fu8e0p	30	23	specificity	specificity	NN
cord-265978-i0fu8e0p	30	24	and	and	CC
cord-265978-i0fu8e0p	30	25	throughput	throughput	NN
cord-265978-i0fu8e0p	30	26	,	,	,
cord-265978-i0fu8e0p	30	27	lower	low	JJR
cord-265978-i0fu8e0p	30	28	hand	hand	NN
cord-265978-i0fu8e0p	30	29	-	-	HYPH
cord-265978-i0fu8e0p	30	30	on	on	IN
cord-265978-i0fu8e0p	30	31	-	-	HYPH
cord-265978-i0fu8e0p	30	32	time	time	NN
cord-265978-i0fu8e0p	30	33	,	,	,
cord-265978-i0fu8e0p	30	34	minimal	minimal	JJ
cord-265978-i0fu8e0p	30	35	contamination	contamination	NN
cord-265978-i0fu8e0p	30	36	rate	rate	NN
cord-265978-i0fu8e0p	30	37	,	,	,
cord-265978-i0fu8e0p	30	38	quantitative	quantitative	JJ
cord-265978-i0fu8e0p	30	39	measurement	measurement	NN
cord-265978-i0fu8e0p	30	40	,	,	,
cord-265978-i0fu8e0p	30	41	automation	automation	NN
cord-265978-i0fu8e0p	30	42	,	,	,
cord-265978-i0fu8e0p	30	43	and	and	CC
cord-265978-i0fu8e0p	30	44	easy	easy	JJ
cord-265978-i0fu8e0p	30	45	standardization	standardization	NN
cord-265978-i0fu8e0p	30	46	[	[	-LRB-
cord-265978-i0fu8e0p	30	47	11	11	CD
cord-265978-i0fu8e0p	30	48	]	]	-RRB-
cord-265978-i0fu8e0p	30	49	.	.	.
cord-265978-i0fu8e0p	31	1	All	all	DT
cord-265978-i0fu8e0p	31	2	real	real	JJ
cord-265978-i0fu8e0p	31	3	-	-	HYPH
cord-265978-i0fu8e0p	31	4	time	time	NN
cord-265978-i0fu8e0p	31	5	amplification	amplification	NN
cord-265978-i0fu8e0p	31	6	methods	method	NNS
cord-265978-i0fu8e0p	31	7	depend	depend	VBP
cord-265978-i0fu8e0p	31	8	on	on	IN
cord-265978-i0fu8e0p	31	9	the	the	DT
cord-265978-i0fu8e0p	31	10	detection	detection	NN
cord-265978-i0fu8e0p	31	11	and	and	CC
cord-265978-i0fu8e0p	31	12	quantification	quantification	NN
cord-265978-i0fu8e0p	31	13	of	of	IN
cord-265978-i0fu8e0p	31	14	a	a	DT
cord-265978-i0fu8e0p	31	15	fluorescent	fluorescent	JJ
cord-265978-i0fu8e0p	31	16	reporter	reporter	NN
cord-265978-i0fu8e0p	31	17	molecule	molecule	NN
cord-265978-i0fu8e0p	31	18	whose	whose	WP$
cord-265978-i0fu8e0p	31	19	signal	signal	NN
cord-265978-i0fu8e0p	31	20	increases	increase	NNS
cord-265978-i0fu8e0p	31	21	in	in	IN
cord-265978-i0fu8e0p	31	22	proportion	proportion	NN
cord-265978-i0fu8e0p	31	23	to	to	IN
cord-265978-i0fu8e0p	31	24	the	the	DT
cord-265978-i0fu8e0p	31	25	generated	generate	VBN
cord-265978-i0fu8e0p	31	26	amplicon	amplicon	NN
cord-265978-i0fu8e0p	31	27	.	.	.
cord-265978-i0fu8e0p	32	1	The	the	DT
cord-265978-i0fu8e0p	32	2	simplest	simple	JJS
cord-265978-i0fu8e0p	32	3	and	and	CC
cord-265978-i0fu8e0p	32	4	most	most	RBS
cord-265978-i0fu8e0p	32	5	cost	cost	NN
cord-265978-i0fu8e0p	32	6	-	-	HYPH
cord-265978-i0fu8e0p	32	7	effective	effective	JJ
cord-265978-i0fu8e0p	32	8	method	method	NN
cord-265978-i0fu8e0p	32	9	involves	involve	VBZ
cord-265978-i0fu8e0p	32	10	the	the	DT
cord-265978-i0fu8e0p	32	11	use	use	NN
cord-265978-i0fu8e0p	32	12	of	of	IN
cord-265978-i0fu8e0p	32	13	DNA	dna	NN
cord-265978-i0fu8e0p	32	14	intercalating	intercalating	NN
cord-265978-i0fu8e0p	32	15	dye	dye	NN
cord-265978-i0fu8e0p	32	16	such	such	JJ
cord-265978-i0fu8e0p	32	17	as	as	IN
cord-265978-i0fu8e0p	32	18	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	32	19	Green	Green	NNP
cord-265978-i0fu8e0p	32	20	I	I	NNP
cord-265978-i0fu8e0p	32	21	as	as	IN
cord-265978-i0fu8e0p	32	22	a	a	DT
cord-265978-i0fu8e0p	32	23	fluorescent	fluorescent	JJ
cord-265978-i0fu8e0p	32	24	reporter	reporter	NN
cord-265978-i0fu8e0p	32	25	.	.	.
cord-265978-i0fu8e0p	33	1	However	however	RB
cord-265978-i0fu8e0p	33	2	,	,	,
cord-265978-i0fu8e0p	33	3	the	the	DT
cord-265978-i0fu8e0p	33	4	major	major	JJ
cord-265978-i0fu8e0p	33	5	disadvantage	disadvantage	NN
cord-265978-i0fu8e0p	33	6	is	be	VBZ
cord-265978-i0fu8e0p	33	7	that	that	IN
cord-265978-i0fu8e0p	33	8	the	the	DT
cord-265978-i0fu8e0p	33	9	dye	dye	NN
cord-265978-i0fu8e0p	33	10	molecules	molecule	NNS
cord-265978-i0fu8e0p	33	11	may	may	MD
cord-265978-i0fu8e0p	33	12	bind	bind	VB
cord-265978-i0fu8e0p	33	13	to	to	IN
cord-265978-i0fu8e0p	33	14	any	any	DT
cord-265978-i0fu8e0p	33	15	double	double	JJ
cord-265978-i0fu8e0p	33	16	stranded	strand	VBN
cord-265978-i0fu8e0p	33	17	DNA	dna	NN
cord-265978-i0fu8e0p	33	18	in	in	IN
cord-265978-i0fu8e0p	33	19	the	the	DT
cord-265978-i0fu8e0p	33	20	reaction	reaction	NN
cord-265978-i0fu8e0p	33	21	including	include	VBG
cord-265978-i0fu8e0p	33	22	non	non	JJ
cord-265978-i0fu8e0p	33	23	-	-	JJ
cord-265978-i0fu8e0p	33	24	specific	specific	JJ
cord-265978-i0fu8e0p	33	25	products	product	NNS
cord-265978-i0fu8e0p	33	26	and	and	CC
cord-265978-i0fu8e0p	33	27	primer	primer	NN
cord-265978-i0fu8e0p	33	28	-	-	HYPH
cord-265978-i0fu8e0p	33	29	dimmers	dimmer	NNS
cord-265978-i0fu8e0p	33	30	,	,	,
cord-265978-i0fu8e0p	33	31	which	which	WDT
cord-265978-i0fu8e0p	33	32	requires	require	VBZ
cord-265978-i0fu8e0p	33	33	melt	melt	NN
cord-265978-i0fu8e0p	33	34	curve	curve	NN
cord-265978-i0fu8e0p	33	35	analysis	analysis	NN
cord-265978-i0fu8e0p	33	36	to	to	TO
cord-265978-i0fu8e0p	33	37	control	control	VB
cord-265978-i0fu8e0p	33	38	the	the	DT
cord-265978-i0fu8e0p	33	39	specificity	specificity	NN
cord-265978-i0fu8e0p	33	40	of	of	IN
cord-265978-i0fu8e0p	33	41	the	the	DT
cord-265978-i0fu8e0p	33	42	reaction	reaction	NN
cord-265978-i0fu8e0p	33	43	[	[	-LRB-
cord-265978-i0fu8e0p	33	44	12	12	CD
cord-265978-i0fu8e0p	33	45	]	]	-RRB-
cord-265978-i0fu8e0p	33	46	.	.	.
cord-265978-i0fu8e0p	34	1	A	a	DT
cord-265978-i0fu8e0p	34	2	single	single	JJ
cord-265978-i0fu8e0p	34	3	report	report	NN
cord-265978-i0fu8e0p	34	4	described	describe	VBD
cord-265978-i0fu8e0p	34	5	the	the	DT
cord-265978-i0fu8e0p	34	6	use	use	NN
cord-265978-i0fu8e0p	34	7	of	of	IN
cord-265978-i0fu8e0p	34	8	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	34	9	Green	Green	NNP
cord-265978-i0fu8e0p	34	10	I	I	NNP
cord-265978-i0fu8e0p	34	11	for	for	IN
cord-265978-i0fu8e0p	34	12	detection	detection	NN
cord-265978-i0fu8e0p	34	13	of	of	IN
cord-265978-i0fu8e0p	34	14	32	32	CD
cord-265978-i0fu8e0p	34	15	animal	animal	NN
cord-265978-i0fu8e0p	34	16	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	34	17	including	include	VBG
cord-265978-i0fu8e0p	34	18	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	34	19	using	use	VBG
cord-265978-i0fu8e0p	34	20	degenerate	degenerate	JJ
cord-265978-i0fu8e0p	34	21	primers	primer	NNS
cord-265978-i0fu8e0p	34	22	[	[	-LRB-
cord-265978-i0fu8e0p	34	23	13	13	CD
cord-265978-i0fu8e0p	34	24	]	]	-RRB-
cord-265978-i0fu8e0p	34	25	.	.	.
cord-265978-i0fu8e0p	35	1	This	this	DT
cord-265978-i0fu8e0p	35	2	test	test	NN
cord-265978-i0fu8e0p	35	3	could	could	MD
cord-265978-i0fu8e0p	35	4	be	be	VB
cord-265978-i0fu8e0p	35	5	used	use	VBN
cord-265978-i0fu8e0p	35	6	only	only	RB
cord-265978-i0fu8e0p	35	7	for	for	IN
cord-265978-i0fu8e0p	35	8	detection	detection	NN
cord-265978-i0fu8e0p	35	9	of	of	IN
cord-265978-i0fu8e0p	35	10	new	new	JJ
cord-265978-i0fu8e0p	35	11	,	,	,
cord-265978-i0fu8e0p	35	12	uncharacterized	uncharacterized	JJ
cord-265978-i0fu8e0p	35	13	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	35	14	rather	rather	RB
cord-265978-i0fu8e0p	35	15	than	than	IN
cord-265978-i0fu8e0p	35	16	for	for	IN
cord-265978-i0fu8e0p	35	17	specific	specific	JJ
cord-265978-i0fu8e0p	35	18	identification	identification	NN
cord-265978-i0fu8e0p	35	19	of	of	IN
cord-265978-i0fu8e0p	35	20	BCoV.	bcov.	CD
cord-265978-i0fu8e0p	36	1	In	in	IN
cord-265978-i0fu8e0p	36	2	this	this	DT
cord-265978-i0fu8e0p	36	3	report	report	NN
cord-265978-i0fu8e0p	36	4	,	,	,
cord-265978-i0fu8e0p	36	5	a	a	DT
cord-265978-i0fu8e0p	36	6	real	real	JJ
cord-265978-i0fu8e0p	36	7	-	-	HYPH
cord-265978-i0fu8e0p	36	8	time	time	NN
cord-265978-i0fu8e0p	36	9	RT	rt	NN
cord-265978-i0fu8e0p	36	10	-	-	HYPH
cord-265978-i0fu8e0p	36	11	PCR	PCR	NNP
cord-265978-i0fu8e0p	36	12	system	system	NN
cord-265978-i0fu8e0p	36	13	using	use	VBG
cord-265978-i0fu8e0p	36	14	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	36	15	Green	Green	NNP
cord-265978-i0fu8e0p	36	16	I	I	NNP
cord-265978-i0fu8e0p	36	17	and	and	CC
cord-265978-i0fu8e0p	36	18	melt	melt	VB
cord-265978-i0fu8e0p	36	19	curve	curve	NN
cord-265978-i0fu8e0p	36	20	analysis	analysis	NN
cord-265978-i0fu8e0p	36	21	was	be	VBD
cord-265978-i0fu8e0p	36	22	developed	develop	VBN
cord-265978-i0fu8e0p	36	23	for	for	IN
cord-265978-i0fu8e0p	36	24	detection	detection	NN
cord-265978-i0fu8e0p	36	25	and	and	CC
cord-265978-i0fu8e0p	36	26	quantification	quantification	NN
cord-265978-i0fu8e0p	36	27	of	of	IN
cord-265978-i0fu8e0p	36	28	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	36	29	in	in	IN
cord-265978-i0fu8e0p	36	30	clinical	clinical	JJ
cord-265978-i0fu8e0p	36	31	samples	sample	NNS
cord-265978-i0fu8e0p	36	32	.	.	.
cord-265978-i0fu8e0p	37	1	The	the	DT
cord-265978-i0fu8e0p	37	2	assay	assay	NN
cord-265978-i0fu8e0p	37	3	performance	performance	NN
cord-265978-i0fu8e0p	37	4	was	be	VBD
cord-265978-i0fu8e0p	37	5	verified	verify	VBN
cord-265978-i0fu8e0p	37	6	by	by	IN
cord-265978-i0fu8e0p	37	7	the	the	DT
cord-265978-i0fu8e0p	37	8	evaluation	evaluation	NN
cord-265978-i0fu8e0p	37	9	of	of	IN
cord-265978-i0fu8e0p	37	10	specificity	specificity	NN
cord-265978-i0fu8e0p	37	11	,	,	,
cord-265978-i0fu8e0p	37	12	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	37	13	,	,	,
cord-265978-i0fu8e0p	37	14	reproducibility	reproducibility	NN
cord-265978-i0fu8e0p	37	15	and	and	CC
cord-265978-i0fu8e0p	37	16	dynamic	dynamic	JJ
cord-265978-i0fu8e0p	37	17	range	range	NN
cord-265978-i0fu8e0p	37	18	.	.	.
cord-265978-i0fu8e0p	38	1	The	the	DT
cord-265978-i0fu8e0p	38	2	different	different	JJ
cord-265978-i0fu8e0p	38	3	viruses	virus	NNS
cord-265978-i0fu8e0p	38	4	used	use	VBN
cord-265978-i0fu8e0p	38	5	in	in	IN
cord-265978-i0fu8e0p	38	6	this	this	DT
cord-265978-i0fu8e0p	38	7	study	study	NN
cord-265978-i0fu8e0p	38	8	are	be	VBP
cord-265978-i0fu8e0p	38	9	listed	list	VBN
cord-265978-i0fu8e0p	38	10	in	in	IN
cord-265978-i0fu8e0p	38	11	Table	Table	NNP
cord-265978-i0fu8e0p	38	12	1	1	CD
cord-265978-i0fu8e0p	38	13	.	.	.
cord-265978-i0fu8e0p	39	1	BCoV	bcov	NN
cord-265978-i0fu8e0p	39	2	reference	reference	NN
cord-265978-i0fu8e0p	39	3	(	(	-LRB-
cord-265978-i0fu8e0p	39	4	Mebus	Mebus	NNP
cord-265978-i0fu8e0p	39	5	)	)	-RRB-
cord-265978-i0fu8e0p	39	6	strain	strain	NN
cord-265978-i0fu8e0p	39	7	was	be	VBD
cord-265978-i0fu8e0p	39	8	used	use	VBN
cord-265978-i0fu8e0p	39	9	throughout	throughout	IN
cord-265978-i0fu8e0p	39	10	this	this	DT
cord-265978-i0fu8e0p	39	11	study	study	NN
cord-265978-i0fu8e0p	39	12	to	to	TO
cord-265978-i0fu8e0p	39	13	standardize	standardize	VB
cord-265978-i0fu8e0p	39	14	the	the	DT
cord-265978-i0fu8e0p	39	15	real	real	JJ
cord-265978-i0fu8e0p	39	16	-	-	HYPH
cord-265978-i0fu8e0p	39	17	time	time	NN
cord-265978-i0fu8e0p	39	18	RT	RT	NNP
cord-265978-i0fu8e0p	39	19	-	-	HYPH
cord-265978-i0fu8e0p	39	20	PCR	PCR	NNP
cord-265978-i0fu8e0p	39	21	assay	assay	NN
cord-265978-i0fu8e0p	39	22	.	.	.
cord-265978-i0fu8e0p	40	1	Several	several	JJ
cord-265978-i0fu8e0p	40	2	enteric	enteric	JJ
cord-265978-i0fu8e0p	40	3	and	and	CC
cord-265978-i0fu8e0p	40	4	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	40	5	bovine	bovine	JJ
cord-265978-i0fu8e0p	40	6	viruses	virus	NNS
cord-265978-i0fu8e0p	40	7	were	be	VBD
cord-265978-i0fu8e0p	40	8	included	include	VBN
cord-265978-i0fu8e0p	40	9	to	to	TO
cord-265978-i0fu8e0p	40	10	test	test	VB
cord-265978-i0fu8e0p	40	11	the	the	DT
cord-265978-i0fu8e0p	40	12	specificity	specificity	NN
cord-265978-i0fu8e0p	40	13	of	of	IN
cord-265978-i0fu8e0p	40	14	the	the	DT
cord-265978-i0fu8e0p	40	15	developed	developed	JJ
cord-265978-i0fu8e0p	40	16	assay	assay	NN
cord-265978-i0fu8e0p	40	17	.	.	.
cord-265978-i0fu8e0p	41	1	A	a	DT
cord-265978-i0fu8e0p	41	2	single	single	JJ
cord-265978-i0fu8e0p	41	3	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	41	4	(	(	-LRB-
cord-265978-i0fu8e0p	41	5	avian	avian	JJ
cord-265978-i0fu8e0p	41	6	infectious	infectious	JJ
cord-265978-i0fu8e0p	41	7	bronchitis	bronchitis	NN
cord-265978-i0fu8e0p	41	8	virus	virus	NN
cord-265978-i0fu8e0p	41	9	)	)	-RRB-
cord-265978-i0fu8e0p	41	10	was	be	VBD
cord-265978-i0fu8e0p	41	11	used	use	VBN
cord-265978-i0fu8e0p	41	12	for	for	IN
cord-265978-i0fu8e0p	41	13	evaluation	evaluation	NN
cord-265978-i0fu8e0p	41	14	of	of	IN
cord-265978-i0fu8e0p	41	15	the	the	DT
cord-265978-i0fu8e0p	41	16	cross	cross	NN
cord-265978-i0fu8e0p	41	17	reactivity	reactivity	NN
cord-265978-i0fu8e0p	41	18	.	.	.
cord-265978-i0fu8e0p	42	1	Other	other	JJ
cord-265978-i0fu8e0p	42	2	viruses	virus	NNS
cord-265978-i0fu8e0p	42	3	were	be	VBD
cord-265978-i0fu8e0p	42	4	included	include	VBN
cord-265978-i0fu8e0p	42	5	as	as	IN
cord-265978-i0fu8e0p	42	6	negative	negative	JJ
cord-265978-i0fu8e0p	42	7	controls	control	NNS
cord-265978-i0fu8e0p	42	8	.	.	.
cord-265978-i0fu8e0p	43	1	A	a	DT
cord-265978-i0fu8e0p	43	2	total	total	NN
cord-265978-i0fu8e0p	43	3	of	of	IN
cord-265978-i0fu8e0p	43	4	103	103	CD
cord-265978-i0fu8e0p	43	5	bovine	bovine	JJ
cord-265978-i0fu8e0p	43	6	clinical	clinical	JJ
cord-265978-i0fu8e0p	43	7	samples	sample	NNS
cord-265978-i0fu8e0p	43	8	were	be	VBD
cord-265978-i0fu8e0p	43	9	collected	collect	VBN
cord-265978-i0fu8e0p	43	10	from	from	IN
cord-265978-i0fu8e0p	43	11	affected	affected	JJ
cord-265978-i0fu8e0p	43	12	cases	case	NNS
cord-265978-i0fu8e0p	43	13	with	with	IN
cord-265978-i0fu8e0p	43	14	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	43	15	and/or	and/or	CC
cord-265978-i0fu8e0p	43	16	enteric	enteric	JJ
cord-265978-i0fu8e0p	43	17	manifestations	manifestation	NNS
cord-265978-i0fu8e0p	43	18	.	.	.
cord-265978-i0fu8e0p	44	1	These	these	DT
cord-265978-i0fu8e0p	44	2	samples	sample	NNS
cord-265978-i0fu8e0p	44	3	included	include	VBD
cord-265978-i0fu8e0p	44	4	68	68	CD
cord-265978-i0fu8e0p	44	5	fecal	fecal	JJ
cord-265978-i0fu8e0p	44	6	and	and	CC
cord-265978-i0fu8e0p	44	7	35	35	CD
cord-265978-i0fu8e0p	44	8	nasal	nasal	NN
cord-265978-i0fu8e0p	44	9	swabs	swab	NNS
cord-265978-i0fu8e0p	44	10	.	.	.
cord-265978-i0fu8e0p	45	1	Swabs	swab	NNS
cord-265978-i0fu8e0p	45	2	from	from	IN
cord-265978-i0fu8e0p	45	3	BCoVfree	bcovfree	JJ
cord-265978-i0fu8e0p	45	4	calves	calf	NNS
cord-265978-i0fu8e0p	45	5	were	be	VBD
cord-265978-i0fu8e0p	45	6	also	also	RB
cord-265978-i0fu8e0p	45	7	included	include	VBN
cord-265978-i0fu8e0p	45	8	to	to	TO
cord-265978-i0fu8e0p	45	9	serve	serve	VB
cord-265978-i0fu8e0p	45	10	as	as	IN
cord-265978-i0fu8e0p	45	11	negative	negative	JJ
cord-265978-i0fu8e0p	45	12	controls	control	NNS
cord-265978-i0fu8e0p	45	13	and	and	CC
cord-265978-i0fu8e0p	45	14	matrices	matrix	NNS
cord-265978-i0fu8e0p	45	15	for	for	IN
cord-265978-i0fu8e0p	45	16	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	45	17	testing	testing	NN
cord-265978-i0fu8e0p	45	18	.	.	.
cord-265978-i0fu8e0p	46	1	Immediately	immediately	RB
cord-265978-i0fu8e0p	46	2	after	after	IN
cord-265978-i0fu8e0p	46	3	collection	collection	NN
cord-265978-i0fu8e0p	46	4	,	,	,
cord-265978-i0fu8e0p	46	5	swabs	swab	NNS
cord-265978-i0fu8e0p	46	6	were	be	VBD
cord-265978-i0fu8e0p	46	7	placed	place	VBN
cord-265978-i0fu8e0p	46	8	in	in	IN
cord-265978-i0fu8e0p	46	9	1	1	CD
cord-265978-i0fu8e0p	46	10	ml	ml	NN
cord-265978-i0fu8e0p	46	11	viral	viral	JJ
cord-265978-i0fu8e0p	46	12	transport	transport	NN
cord-265978-i0fu8e0p	46	13	medium	medium	NN
cord-265978-i0fu8e0p	46	14	consisting	consist	VBG
cord-265978-i0fu8e0p	46	15	of	of	IN
cord-265978-i0fu8e0p	46	16	Minimal	minimal	JJ
cord-265978-i0fu8e0p	46	17	essential	essential	JJ
cord-265978-i0fu8e0p	46	18	medium	medium	NN
cord-265978-i0fu8e0p	46	19	(	(	-LRB-
cord-265978-i0fu8e0p	46	20	MEM	MEM	NNP
cord-265978-i0fu8e0p	46	21	)	)	-RRB-
cord-265978-i0fu8e0p	46	22	,	,	,
cord-265978-i0fu8e0p	46	23	500	500	CD
cord-265978-i0fu8e0p	46	24	U	u	NN
cord-265978-i0fu8e0p	46	25	penicillin	penicillin	NN
cord-265978-i0fu8e0p	46	26	and	and	CC
cord-265978-i0fu8e0p	46	27	500	500	CD
cord-265978-i0fu8e0p	46	28	mg	mg	NN
cord-265978-i0fu8e0p	46	29	streptomycin	streptomycin	NNS
cord-265978-i0fu8e0p	46	30	;	;	,
cord-265978-i0fu8e0p	46	31	transported	transport	VBN
cord-265978-i0fu8e0p	46	32	on	on	IN
cord-265978-i0fu8e0p	46	33	dry	dry	JJ
cord-265978-i0fu8e0p	46	34	ice	ice	NN
cord-265978-i0fu8e0p	46	35	and	and	CC
cord-265978-i0fu8e0p	46	36	stored	store	VBN
cord-265978-i0fu8e0p	46	37	directly	directly	RB
cord-265978-i0fu8e0p	46	38	at	at	IN
cord-265978-i0fu8e0p	46	39	À80	À80	NNP
cord-265978-i0fu8e0p	46	40	C	C	NNP
cord-265978-i0fu8e0p	46	41	until	until	IN
cord-265978-i0fu8e0p	46	42	processing	processing	NN
cord-265978-i0fu8e0p	46	43	.	.	.
cord-265978-i0fu8e0p	47	1	For	for	IN
cord-265978-i0fu8e0p	47	2	sample	sample	NN
cord-265978-i0fu8e0p	47	3	preparation	preparation	NN
cord-265978-i0fu8e0p	47	4	,	,	,
cord-265978-i0fu8e0p	47	5	all	all	DT
cord-265978-i0fu8e0p	47	6	swabs	swab	NNS
cord-265978-i0fu8e0p	47	7	were	be	VBD
cord-265978-i0fu8e0p	47	8	discarded	discard	VBN
cord-265978-i0fu8e0p	47	9	after	after	IN
cord-265978-i0fu8e0p	47	10	pulse	pulse	NN
cord-265978-i0fu8e0p	47	11	-	-	HYPH
cord-265978-i0fu8e0p	47	12	vortexing	vortexing	NN
cord-265978-i0fu8e0p	47	13	for	for	IN
cord-265978-i0fu8e0p	47	14	15	15	CD
cord-265978-i0fu8e0p	47	15	s	s	NN
cord-265978-i0fu8e0p	47	16	;	;	:
cord-265978-i0fu8e0p	47	17	sample	sample	NN
cord-265978-i0fu8e0p	47	18	suspension	suspension	NN
cord-265978-i0fu8e0p	47	19	was	be	VBD
cord-265978-i0fu8e0p	47	20	diluted	dilute	VBN
cord-265978-i0fu8e0p	47	21	1:10	1:10	CD
cord-265978-i0fu8e0p	47	22	in	in	IN
cord-265978-i0fu8e0p	47	23	MEM	mem	NN
cord-265978-i0fu8e0p	47	24	and	and	CC
cord-265978-i0fu8e0p	47	25	clarified	clarify	VBN
cord-265978-i0fu8e0p	47	26	by	by	IN
cord-265978-i0fu8e0p	47	27	centrifugation	centrifugation	NN
cord-265978-i0fu8e0p	47	28	at	at	IN
cord-265978-i0fu8e0p	47	29	5000	5000	CD
cord-265978-i0fu8e0p	47	30	rpm	rpm	NN
cord-265978-i0fu8e0p	47	31	for	for	IN
cord-265978-i0fu8e0p	47	32	15	15	CD
cord-265978-i0fu8e0p	47	33	min	min	NN
cord-265978-i0fu8e0p	47	34	at	at	IN
cord-265978-i0fu8e0p	47	35	4	4	CD
cord-265978-i0fu8e0p	47	36	C.	c.	NN
cord-265978-i0fu8e0p	48	1	The	the	DT
cord-265978-i0fu8e0p	48	2	supernatant	supernatant	NN
cord-265978-i0fu8e0p	48	3	was	be	VBD
cord-265978-i0fu8e0p	48	4	transferred	transfer	VBN
cord-265978-i0fu8e0p	48	5	to	to	IN
cord-265978-i0fu8e0p	48	6	sterile	sterile	JJ
cord-265978-i0fu8e0p	48	7	vials	vial	NNS
cord-265978-i0fu8e0p	48	8	where	where	WRB
cord-265978-i0fu8e0p	48	9	aliquots	aliquot	NNS
cord-265978-i0fu8e0p	48	10	of	of	IN
cord-265978-i0fu8e0p	48	11	140	140	CD
cord-265978-i0fu8e0p	48	12	ml	ml	NNS
cord-265978-i0fu8e0p	48	13	were	be	VBD
cord-265978-i0fu8e0p	48	14	used	use	VBN
cord-265978-i0fu8e0p	48	15	for	for	IN
cord-265978-i0fu8e0p	48	16	viral	viral	JJ
cord-265978-i0fu8e0p	48	17	RNA	RNA	NNP
cord-265978-i0fu8e0p	48	18	extraction	extraction	NN
cord-265978-i0fu8e0p	48	19	and	and	CC
cord-265978-i0fu8e0p	48	20	the	the	DT
cord-265978-i0fu8e0p	48	21	rest	rest	NN
cord-265978-i0fu8e0p	48	22	was	be	VBD
cord-265978-i0fu8e0p	48	23	stored	store	VBN
cord-265978-i0fu8e0p	48	24	at	at	IN
cord-265978-i0fu8e0p	48	25	À80	À80	NNP
cord-265978-i0fu8e0p	48	26	C.	C.	NNP
cord-265978-i0fu8e0p	49	1	The	the	DT
cord-265978-i0fu8e0p	49	2	N	n	CD
cord-265978-i0fu8e0p	49	3	gene	gene	NN
cord-265978-i0fu8e0p	49	4	sequences	sequence	NNS
cord-265978-i0fu8e0p	49	5	from	from	IN
cord-265978-i0fu8e0p	49	6	28	28	CD
cord-265978-i0fu8e0p	49	7	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	49	8	known	know	VBN
cord-265978-i0fu8e0p	49	9	isolates	isolate	NNS
cord-265978-i0fu8e0p	49	10	worldwide	worldwide	RB
cord-265978-i0fu8e0p	49	11	were	be	VBD
cord-265978-i0fu8e0p	49	12	aligned	align	VBN
cord-265978-i0fu8e0p	49	13	with	with	IN
cord-265978-i0fu8e0p	49	14	the	the	DT
cord-265978-i0fu8e0p	49	15	Clustal	Clustal	NNP
cord-265978-i0fu8e0p	49	16	W	W	NNP
cord-265978-i0fu8e0p	49	17	method	method	NN
cord-265978-i0fu8e0p	49	18	available	available	JJ
cord-265978-i0fu8e0p	49	19	as	as	IN
cord-265978-i0fu8e0p	49	20	part	part	NN
cord-265978-i0fu8e0p	49	21	of	of	IN
cord-265978-i0fu8e0p	49	22	the	the	DT
cord-265978-i0fu8e0p	49	23	Megalign	Megalign	NNP
cord-265978-i0fu8e0p	49	24	program	program	NN
cord-265978-i0fu8e0p	49	25	in	in	IN
cord-265978-i0fu8e0p	49	26	the	the	DT
cord-265978-i0fu8e0p	49	27	Lasergene	Lasergene	NNP
cord-265978-i0fu8e0p	49	28	sequence	sequence	NN
cord-265978-i0fu8e0p	49	29	analysis	analysis	NN
cord-265978-i0fu8e0p	49	30	package	package	NN
cord-265978-i0fu8e0p	49	31	version	version	NN
cord-265978-i0fu8e0p	49	32	3.18	3.18	CD
cord-265978-i0fu8e0p	49	33	(	(	-LRB-
cord-265978-i0fu8e0p	49	34	DNAStar	dnastar	NN
cord-265978-i0fu8e0p	49	35	,	,	,
cord-265978-i0fu8e0p	49	36	Madison	Madison	NNP
cord-265978-i0fu8e0p	49	37	,	,	,
cord-265978-i0fu8e0p	49	38	WI	WI	NNP
cord-265978-i0fu8e0p	49	39	,	,	,
cord-265978-i0fu8e0p	49	40	USA	USA	NNP
cord-265978-i0fu8e0p	49	41	)	)	-RRB-
cord-265978-i0fu8e0p	49	42	.	.	.
cord-265978-i0fu8e0p	50	1	A	a	DT
cord-265978-i0fu8e0p	50	2	specific	specific	JJ
cord-265978-i0fu8e0p	50	3	oligonucleotide	oligonucleotide	JJ
cord-265978-i0fu8e0p	50	4	primer	primer	NN
cord-265978-i0fu8e0p	50	5	set	set	NN
cord-265978-i0fu8e0p	50	6	was	be	VBD
cord-265978-i0fu8e0p	50	7	designed	design	VBN
cord-265978-i0fu8e0p	50	8	with	with	IN
cord-265978-i0fu8e0p	50	9	the	the	DT
cord-265978-i0fu8e0p	50	10	Primer3	Primer3	NNP
cord-265978-i0fu8e0p	50	11	program	program	NN
cord-265978-i0fu8e0p	50	12	(	(	-LRB-
cord-265978-i0fu8e0p	50	13	Agilent	Agilent	NNP
cord-265978-i0fu8e0p	50	14	Technologies	Technologies	NNP
cord-265978-i0fu8e0p	50	15	,	,	,
cord-265978-i0fu8e0p	50	16	Santa	Santa	NNP
cord-265978-i0fu8e0p	50	17	Clara	Clara	NNP
cord-265978-i0fu8e0p	50	18	,	,	,
cord-265978-i0fu8e0p	50	19	CA	CA	NNP
cord-265978-i0fu8e0p	50	20	,	,	,
cord-265978-i0fu8e0p	50	21	USA	USA	NNP
cord-265978-i0fu8e0p	50	22	)	)	-RRB-
cord-265978-i0fu8e0p	50	23	for	for	IN
cord-265978-i0fu8e0p	50	24	use	use	NN
cord-265978-i0fu8e0p	50	25	in	in	IN
cord-265978-i0fu8e0p	50	26	real	real	JJ
cord-265978-i0fu8e0p	50	27	-	-	HYPH
cord-265978-i0fu8e0p	50	28	time	time	NN
cord-265978-i0fu8e0p	50	29	PCR	PCR	NNP
cord-265978-i0fu8e0p	50	30	to	to	TO
cord-265978-i0fu8e0p	50	31	amplify	amplify	VB
cord-265978-i0fu8e0p	50	32	as	as	RB
cord-265978-i0fu8e0p	50	33	many	many	JJ
cord-265978-i0fu8e0p	50	34	as	as	IN
cord-265978-i0fu8e0p	50	35	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	50	36	strains	strain	NNS
cord-265978-i0fu8e0p	50	37	as	as	RB
cord-265978-i0fu8e0p	50	38	theoretically	theoretically	RB
cord-265978-i0fu8e0p	50	39	possible	possible	JJ
cord-265978-i0fu8e0p	50	40	.	.	.
cord-265978-i0fu8e0p	51	1	No	no	DT
cord-265978-i0fu8e0p	51	2	sequence	sequence	NN
cord-265978-i0fu8e0p	51	3	homology	homology	NN
cord-265978-i0fu8e0p	51	4	revealed	reveal	VBD
cord-265978-i0fu8e0p	51	5	using	use	VBG
cord-265978-i0fu8e0p	51	6	these	these	DT
cord-265978-i0fu8e0p	51	7	primers	primer	NNS
cord-265978-i0fu8e0p	51	8	with	with	IN
cord-265978-i0fu8e0p	51	9	group	group	NN
cord-265978-i0fu8e0p	51	10	1	1	CD
cord-265978-i0fu8e0p	51	11	and	and	CC
cord-265978-i0fu8e0p	51	12	3	3	CD
cord-265978-i0fu8e0p	51	13	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	51	14	,	,	,
cord-265978-i0fu8e0p	51	15	however	however	RB
cord-265978-i0fu8e0p	51	16	,	,	,
cord-265978-i0fu8e0p	51	17	cross	cross	VBP
cord-265978-i0fu8e0p	51	18	reactivity	reactivity	NN
cord-265978-i0fu8e0p	51	19	with	with	IN
cord-265978-i0fu8e0p	51	20	members	member	NNS
cord-265978-i0fu8e0p	51	21	of	of	IN
cord-265978-i0fu8e0p	51	22	group	group	NN
cord-265978-i0fu8e0p	51	23	2	2	CD
cord-265978-i0fu8e0p	51	24	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	51	25	is	be	VBZ
cord-265978-i0fu8e0p	51	26	expected	expect	VBN
cord-265978-i0fu8e0p	51	27	.	.	.
cord-265978-i0fu8e0p	52	1	Previously	previously	RB
cord-265978-i0fu8e0p	52	2	described	describe	VBN
cord-265978-i0fu8e0p	52	3	primers	primer	NNS
cord-265978-i0fu8e0p	52	4	for	for	IN
cord-265978-i0fu8e0p	52	5	amplification	amplification	NN
cord-265978-i0fu8e0p	52	6	of	of	IN
cord-265978-i0fu8e0p	52	7	the	the	DT
cord-265978-i0fu8e0p	52	8	entire	entire	JJ
cord-265978-i0fu8e0p	52	9	N	n	CD
cord-265978-i0fu8e0p	52	10	gene	gene	NN
cord-265978-i0fu8e0p	52	11	were	be	VBD
cord-265978-i0fu8e0p	52	12	used	use	VBN
cord-265978-i0fu8e0p	52	13	in	in	IN
cord-265978-i0fu8e0p	52	14	construction	construction	NN
cord-265978-i0fu8e0p	52	15	of	of	IN
cord-265978-i0fu8e0p	52	16	the	the	DT
cord-265978-i0fu8e0p	52	17	quantification	quantification	NN
cord-265978-i0fu8e0p	52	18	standards	standard	NNS
cord-265978-i0fu8e0p	53	1	(	(	-LRB-
cord-265978-i0fu8e0p	53	2	Amer	Amer	NNP
cord-265978-i0fu8e0p	53	3	et	et	NNP
cord-265978-i0fu8e0p	53	4	al	al	NNP
cord-265978-i0fu8e0p	53	5	.	.	NNP
cord-265978-i0fu8e0p	53	6	,	,	,
cord-265978-i0fu8e0p	53	7	2008	2008	CD
cord-265978-i0fu8e0p	53	8	)	)	-RRB-
cord-265978-i0fu8e0p	53	9	.	.	.
cord-265978-i0fu8e0p	54	1	Internal	internal	JJ
cord-265978-i0fu8e0p	54	2	control	control	NN
cord-265978-i0fu8e0p	54	3	primers	primer	NNS
cord-265978-i0fu8e0p	54	4	were	be	VBD
cord-265978-i0fu8e0p	54	5	designed	design	VBN
cord-265978-i0fu8e0p	54	6	on	on	IN
cord-265978-i0fu8e0p	54	7	the	the	DT
cord-265978-i0fu8e0p	54	8	basis	basis	NN
cord-265978-i0fu8e0p	54	9	of	of	IN
cord-265978-i0fu8e0p	54	10	highly	highly	RB
cord-265978-i0fu8e0p	54	11	conserved	conserved	JJ
cord-265978-i0fu8e0p	54	12	sequences	sequence	NNS
cord-265978-i0fu8e0p	54	13	in	in	IN
cord-265978-i0fu8e0p	54	14	the	the	DT
cord-265978-i0fu8e0p	54	15	bovine	bovine	JJ
cord-265978-i0fu8e0p	54	16	glyceraldehyde-3-phosphate	glyceraldehyde-3-phosphate	NNP
cord-265978-i0fu8e0p	54	17	dehydrogenase	dehydrogenase	NN
cord-265978-i0fu8e0p	54	18	(	(	-LRB-
cord-265978-i0fu8e0p	54	19	BGAPDH	BGAPDH	NNP
cord-265978-i0fu8e0p	54	20	)	)	-RRB-
cord-265978-i0fu8e0p	54	21	gene	gene	NN
cord-265978-i0fu8e0p	54	22	for	for	IN
cord-265978-i0fu8e0p	54	23	use	use	NN
cord-265978-i0fu8e0p	54	24	in	in	IN
cord-265978-i0fu8e0p	54	25	validation	validation	NN
cord-265978-i0fu8e0p	54	26	of	of	IN
cord-265978-i0fu8e0p	54	27	RNA	RNA	NNP
cord-265978-i0fu8e0p	54	28	extraction	extraction	NN
cord-265978-i0fu8e0p	54	29	,	,	,
cord-265978-i0fu8e0p	54	30	cDNA	cdna	NN
cord-265978-i0fu8e0p	54	31	synthesis	synthesis	NN
cord-265978-i0fu8e0p	54	32	and	and	CC
cord-265978-i0fu8e0p	54	33	qPCR	qPCR	NNP
cord-265978-i0fu8e0p	54	34	steps	step	NNS
cord-265978-i0fu8e0p	54	35	.	.	.
cord-265978-i0fu8e0p	55	1	All	all	DT
cord-265978-i0fu8e0p	55	2	primers	primer	NNS
cord-265978-i0fu8e0p	55	3	were	be	VBD
cord-265978-i0fu8e0p	55	4	synthesized	synthesize	VBN
cord-265978-i0fu8e0p	55	5	at	at	IN
cord-265978-i0fu8e0p	55	6	Metabion	Metabion	NNP
cord-265978-i0fu8e0p	55	7	(	(	-LRB-
cord-265978-i0fu8e0p	55	8	Martinsried	Martinsried	NNP
cord-265978-i0fu8e0p	55	9	,	,	,
cord-265978-i0fu8e0p	55	10	Germany	Germany	NNP
cord-265978-i0fu8e0p	55	11	)	)	-RRB-
cord-265978-i0fu8e0p	55	12	.	.	.
cord-265978-i0fu8e0p	56	1	Details	detail	NNS
cord-265978-i0fu8e0p	56	2	of	of	IN
cord-265978-i0fu8e0p	56	3	primers	primer	NNS
cord-265978-i0fu8e0p	56	4	sequences	sequence	NNS
cord-265978-i0fu8e0p	56	5	and	and	CC
cord-265978-i0fu8e0p	56	6	locations	location	NNS
cord-265978-i0fu8e0p	56	7	are	be	VBP
cord-265978-i0fu8e0p	56	8	listed	list	VBN
cord-265978-i0fu8e0p	56	9	in	in	IN
cord-265978-i0fu8e0p	56	10	Table	Table	NNP
cord-265978-i0fu8e0p	56	11	2	2	CD
cord-265978-i0fu8e0p	56	12	.	.	.
cord-265978-i0fu8e0p	57	1	Viral	Viral	NNP
cord-265978-i0fu8e0p	57	2	RNA	RNA	NNP
cord-265978-i0fu8e0p	57	3	was	be	VBD
cord-265978-i0fu8e0p	57	4	extracted	extract	VBN
cord-265978-i0fu8e0p	57	5	from	from	IN
cord-265978-i0fu8e0p	57	6	the	the	DT
cord-265978-i0fu8e0p	57	7	different	different	JJ
cord-265978-i0fu8e0p	57	8	virus	virus	NN
cord-265978-i0fu8e0p	57	9	strains	strain	NNS
cord-265978-i0fu8e0p	57	10	and	and	CC
cord-265978-i0fu8e0p	57	11	clinical	clinical	JJ
cord-265978-i0fu8e0p	57	12	samples	sample	NNS
cord-265978-i0fu8e0p	57	13	using	use	VBG
cord-265978-i0fu8e0p	57	14	the	the	DT
cord-265978-i0fu8e0p	57	15	QIAamp	QIAamp	NNP
cord-265978-i0fu8e0p	57	16	viral	viral	JJ
cord-265978-i0fu8e0p	57	17	RNA	RNA	NNP
cord-265978-i0fu8e0p	57	18	extraction	extraction	NN
cord-265978-i0fu8e0p	57	19	kit	kit	NN
cord-265978-i0fu8e0p	57	20	(	(	-LRB-
cord-265978-i0fu8e0p	57	21	Qiagen	Qiagen	NNP
cord-265978-i0fu8e0p	57	22	,	,	,
cord-265978-i0fu8e0p	57	23	Hilden	Hilden	NNP
cord-265978-i0fu8e0p	57	24	,	,	,
cord-265978-i0fu8e0p	57	25	Germany	Germany	NNP
cord-265978-i0fu8e0p	57	26	)	)	-RRB-
cord-265978-i0fu8e0p	57	27	according	accord	VBG
cord-265978-i0fu8e0p	57	28	to	to	IN
cord-265978-i0fu8e0p	57	29	the	the	DT
cord-265978-i0fu8e0p	57	30	manufacturer	manufacturer	NN
cord-265978-i0fu8e0p	57	31	's	's	POS
cord-265978-i0fu8e0p	57	32	protocol	protocol	NN
cord-265978-i0fu8e0p	57	33	.	.	.
cord-265978-i0fu8e0p	58	1	The	the	DT
cord-265978-i0fu8e0p	58	2	RNA	RNA	NNP
cord-265978-i0fu8e0p	58	3	was	be	VBD
cord-265978-i0fu8e0p	58	4	eluted	elute	VBN
cord-265978-i0fu8e0p	58	5	in	in	IN
cord-265978-i0fu8e0p	58	6	60	60	CD
cord-265978-i0fu8e0p	58	7	ml	ml	NN
cord-265978-i0fu8e0p	58	8	elution	elution	NN
cord-265978-i0fu8e0p	58	9	buffer	buffer	NN
cord-265978-i0fu8e0p	58	10	and	and	CC
cord-265978-i0fu8e0p	58	11	used	use	VBN
cord-265978-i0fu8e0p	58	12	directly	directly	RB
cord-265978-i0fu8e0p	58	13	for	for	IN
cord-265978-i0fu8e0p	58	14	reverse	reverse	JJ
cord-265978-i0fu8e0p	58	15	transcription	transcription	NN
cord-265978-i0fu8e0p	58	16	.	.	.
cord-265978-i0fu8e0p	59	1	First	first	JJ
cord-265978-i0fu8e0p	59	2	strand	strand	NN
cord-265978-i0fu8e0p	59	3	cDNA	cdna	NN
cord-265978-i0fu8e0p	59	4	synthesis	synthesis	NN
cord-265978-i0fu8e0p	59	5	was	be	VBD
cord-265978-i0fu8e0p	59	6	performed	perform	VBN
cord-265978-i0fu8e0p	59	7	using	use	VBG
cord-265978-i0fu8e0p	59	8	Sensiscript	Sensiscript	NNP
cord-265978-i0fu8e0p	59	9	Ò	Ò	NNP
cord-265978-i0fu8e0p	59	10	Reverse	Reverse	NNP
cord-265978-i0fu8e0p	59	11	transcription	transcription	NN
cord-265978-i0fu8e0p	59	12	kit	kit	NN
cord-265978-i0fu8e0p	59	13	(	(	-LRB-
cord-265978-i0fu8e0p	59	14	Qiagen	Qiagen	NNP
cord-265978-i0fu8e0p	59	15	)	)	-RRB-
cord-265978-i0fu8e0p	59	16	and	and	CC
cord-265978-i0fu8e0p	59	17	random	random	JJ
cord-265978-i0fu8e0p	59	18	hexameres	hexamere	NNS
cord-265978-i0fu8e0p	59	19	(	(	-LRB-
cord-265978-i0fu8e0p	59	20	Jena	Jena	NNP
cord-265978-i0fu8e0p	59	21	Biosciences	Biosciences	NNP
cord-265978-i0fu8e0p	59	22	Gmbl	Gmbl	NNP
cord-265978-i0fu8e0p	59	23	,	,	,
cord-265978-i0fu8e0p	59	24	Jena	Jena	NNP
cord-265978-i0fu8e0p	59	25	,	,	,
cord-265978-i0fu8e0p	59	26	Germany	Germany	NNP
cord-265978-i0fu8e0p	59	27	)	)	-RRB-
cord-265978-i0fu8e0p	59	28	according	accord	VBG
cord-265978-i0fu8e0p	59	29	to	to	IN
cord-265978-i0fu8e0p	59	30	the	the	DT
cord-265978-i0fu8e0p	59	31	manufacturer	manufacturer	NN
cord-265978-i0fu8e0p	59	32	's	's	POS
cord-265978-i0fu8e0p	59	33	guidelines	guideline	NNS
cord-265978-i0fu8e0p	59	34	.	.	.
cord-265978-i0fu8e0p	60	1	Briefly	briefly	RB
cord-265978-i0fu8e0p	60	2	,	,	,
cord-265978-i0fu8e0p	60	3	5	5	CD
cord-265978-i0fu8e0p	60	4	mL	ml	NN
cord-265978-i0fu8e0p	60	5	of	of	IN
cord-265978-i0fu8e0p	60	6	purified	purify	VBN
cord-265978-i0fu8e0p	60	7	RNA	RNA	NNP
cord-265978-i0fu8e0p	60	8	were	be	VBD
cord-265978-i0fu8e0p	60	9	added	add	VBN
cord-265978-i0fu8e0p	60	10	to	to	IN
cord-265978-i0fu8e0p	60	11	a	a	DT
cord-265978-i0fu8e0p	60	12	mixture	mixture	NN
cord-265978-i0fu8e0p	60	13	contained	contain	VBN
cord-265978-i0fu8e0p	60	14	2	2	CD
cord-265978-i0fu8e0p	60	15	ml	ml	NNS
cord-265978-i0fu8e0p	60	16	of	of	IN
cord-265978-i0fu8e0p	60	17	10Â	10â	CD
cord-265978-i0fu8e0p	60	18	buffer	buffer	NN
cord-265978-i0fu8e0p	60	19	RT	RT	NNP
cord-265978-i0fu8e0p	60	20	,	,	,
cord-265978-i0fu8e0p	60	21	2	2	CD
cord-265978-i0fu8e0p	60	22	ml	ml	NN
cord-265978-i0fu8e0p	60	23	(	(	-LRB-
cord-265978-i0fu8e0p	60	24	0.5	0.5	CD
cord-265978-i0fu8e0p	60	25	mM	mm	CD
cord-265978-i0fu8e0p	60	26	)	)	-RRB-
cord-265978-i0fu8e0p	60	27	of	of	IN
cord-265978-i0fu8e0p	60	28	dNTPs	dntps	CD
cord-265978-i0fu8e0p	60	29	mix	mix	NN
cord-265978-i0fu8e0p	60	30	,	,	,
cord-265978-i0fu8e0p	60	31	2	2	CD
cord-265978-i0fu8e0p	60	32	ml	ml	NN
cord-265978-i0fu8e0p	60	33	(	(	-LRB-
cord-265978-i0fu8e0p	60	34	200	200	CD
cord-265978-i0fu8e0p	60	35	mM	mm	CD
cord-265978-i0fu8e0p	60	36	)	)	-RRB-
cord-265978-i0fu8e0p	60	37	of	of	IN
cord-265978-i0fu8e0p	60	38	random	random	JJ
cord-265978-i0fu8e0p	60	39	hexameres	hexamere	NNS
cord-265978-i0fu8e0p	60	40	,	,	,
cord-265978-i0fu8e0p	60	41	1	1	CD
cord-265978-i0fu8e0p	60	42	ml	ml	NN
cord-265978-i0fu8e0p	60	43	(	(	-LRB-
cord-265978-i0fu8e0p	60	44	4	4	CD
cord-265978-i0fu8e0p	60	45	U	u	NN
cord-265978-i0fu8e0p	60	46	)	)	-RRB-
cord-265978-i0fu8e0p	61	1	RNase	rnase	NN
cord-265978-i0fu8e0p	61	2	inhibitor	inhibitor	NN
cord-265978-i0fu8e0p	61	3	,	,	,
cord-265978-i0fu8e0p	61	4	1	1	CD
cord-265978-i0fu8e0p	61	5	ml	ml	NNS
cord-265978-i0fu8e0p	61	6	of	of	IN
cord-265978-i0fu8e0p	61	7	sensiscript	sensiscript	NNP
cord-265978-i0fu8e0p	61	8	Ò	Ò	NNP
cord-265978-i0fu8e0p	61	9	Reverse	Reverse	NNP
cord-265978-i0fu8e0p	61	10	Transcriptase	Transcriptase	NNP
cord-265978-i0fu8e0p	61	11	and	and	CC
cord-265978-i0fu8e0p	61	12	7	7	CD
cord-265978-i0fu8e0p	61	13	ml	ml	NNS
cord-265978-i0fu8e0p	61	14	of	of	IN
cord-265978-i0fu8e0p	61	15	RNase	RNase	NNP
cord-265978-i0fu8e0p	61	16	free	free	JJ
cord-265978-i0fu8e0p	61	17	water	water	NN
cord-265978-i0fu8e0p	61	18	.	.	.
cord-265978-i0fu8e0p	62	1	After	after	IN
cord-265978-i0fu8e0p	62	2	incubation	incubation	NN
cord-265978-i0fu8e0p	62	3	at	at	IN
cord-265978-i0fu8e0p	62	4	37	37	CD
cord-265978-i0fu8e0p	62	5	C	c	NN
cord-265978-i0fu8e0p	62	6	for	for	IN
cord-265978-i0fu8e0p	62	7	60	60	CD
cord-265978-i0fu8e0p	62	8	min	min	NN
cord-265978-i0fu8e0p	62	9	,	,	,
cord-265978-i0fu8e0p	62	10	the	the	DT
cord-265978-i0fu8e0p	62	11	mixture	mixture	NN
cord-265978-i0fu8e0p	62	12	was	be	VBD
cord-265978-i0fu8e0p	62	13	cooled	cool	VBN
cord-265978-i0fu8e0p	62	14	to	to	IN
cord-265978-i0fu8e0p	62	15	4	4	CD
cord-265978-i0fu8e0p	62	16	C	c	NN
cord-265978-i0fu8e0p	62	17	and	and	CC
cord-265978-i0fu8e0p	62	18	stored	store	VBN
cord-265978-i0fu8e0p	62	19	at	at	IN
cord-265978-i0fu8e0p	62	20	À80	À80	NNP
cord-265978-i0fu8e0p	62	21	C	C	NNP
cord-265978-i0fu8e0p	62	22	until	until	IN
cord-265978-i0fu8e0p	62	23	use	use	NN
cord-265978-i0fu8e0p	62	24	.	.	.
cord-265978-i0fu8e0p	63	1	The	the	DT
cord-265978-i0fu8e0p	63	2	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	63	3	Green	Green	NNP
cord-265978-i0fu8e0p	64	1	I	-PRON-	PRP
cord-265978-i0fu8e0p	64	2	based	base	VBD
cord-265978-i0fu8e0p	64	3	real	real	JJ
cord-265978-i0fu8e0p	64	4	-	-	HYPH
cord-265978-i0fu8e0p	64	5	time	time	NN
cord-265978-i0fu8e0p	64	6	PCR	PCR	NNP
cord-265978-i0fu8e0p	64	7	assay	assay	NN
cord-265978-i0fu8e0p	64	8	was	be	VBD
cord-265978-i0fu8e0p	64	9	performed	perform	VBN
cord-265978-i0fu8e0p	64	10	on	on	IN
cord-265978-i0fu8e0p	64	11	an	an	DT
cord-265978-i0fu8e0p	64	12	ABI	ABI	NNP
cord-265978-i0fu8e0p	64	13	Prism	Prism	NNP
cord-265978-i0fu8e0p	64	14	7500	7500	CD
cord-265978-i0fu8e0p	64	15	Sequence	sequence	NN
cord-265978-i0fu8e0p	64	16	Detection	detection	NN
cord-265978-i0fu8e0p	64	17	System	system	NN
cord-265978-i0fu8e0p	64	18	(	(	-LRB-
cord-265978-i0fu8e0p	64	19	Applied	Applied	NNP
cord-265978-i0fu8e0p	64	20	Biosystems	Biosystems	NNP
cord-265978-i0fu8e0p	64	21	,	,	,
cord-265978-i0fu8e0p	64	22	Foster	Foster	NNP
cord-265978-i0fu8e0p	64	23	City	City	NNP
cord-265978-i0fu8e0p	64	24	,	,	,
cord-265978-i0fu8e0p	64	25	CA	CA	NNP
cord-265978-i0fu8e0p	64	26	,	,	,
cord-265978-i0fu8e0p	64	27	USA	USA	NNP
cord-265978-i0fu8e0p	64	28	)	)	-RRB-
cord-265978-i0fu8e0p	64	29	using	use	VBG
cord-265978-i0fu8e0p	64	30	QuantiTect	QuantiTect	NNP
cord-265978-i0fu8e0p	64	31	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	64	32	Green	Green	NNP
cord-265978-i0fu8e0p	64	33	PCR	PCR	NNP
cord-265978-i0fu8e0p	64	34	Kit	kit	NN
cord-265978-i0fu8e0p	64	35	(	(	-LRB-
cord-265978-i0fu8e0p	64	36	Qiagen	Qiagen	NNP
cord-265978-i0fu8e0p	64	37	)	)	-RRB-
cord-265978-i0fu8e0p	64	38	.	.	.
cord-265978-i0fu8e0p	65	1	The	the	DT
cord-265978-i0fu8e0p	65	2	assay	assay	NN
cord-265978-i0fu8e0p	65	3	was	be	VBD
cord-265978-i0fu8e0p	65	4	carried	carry	VBN
cord-265978-i0fu8e0p	65	5	out	out	RP
cord-265978-i0fu8e0p	65	6	in	in	IN
cord-265978-i0fu8e0p	65	7	a	a	DT
cord-265978-i0fu8e0p	65	8	total	total	JJ
cord-265978-i0fu8e0p	65	9	volume	volume	NN
cord-265978-i0fu8e0p	65	10	of	of	IN
cord-265978-i0fu8e0p	65	11	25	25	CD
cord-265978-i0fu8e0p	65	12	ml	ml	NN
cord-265978-i0fu8e0p	65	13	reaction	reaction	NN
cord-265978-i0fu8e0p	65	14	mixture	mixture	NN
cord-265978-i0fu8e0p	65	15	prepared	prepare	VBN
cord-265978-i0fu8e0p	65	16	in	in	IN
cord-265978-i0fu8e0p	65	17	triplicates	triplicate	NNS
cord-265978-i0fu8e0p	65	18	in	in	IN
cord-265978-i0fu8e0p	65	19	96-well	96-well	CD
cord-265978-i0fu8e0p	65	20	optical	optical	JJ
cord-265978-i0fu8e0p	65	21	reaction	reaction	NN
cord-265978-i0fu8e0p	65	22	plates	plate	NNS
cord-265978-i0fu8e0p	65	23	or	or	CC
cord-265978-i0fu8e0p	65	24	MicroAmp	MicroAmp	NNP
cord-265978-i0fu8e0p	65	25	Ò	ò	NN
cord-265978-i0fu8e0p	65	26	optical	optical	JJ
cord-265978-i0fu8e0p	65	27	tubes	tube	NNS
cord-265978-i0fu8e0p	65	28	(	(	-LRB-
cord-265978-i0fu8e0p	65	29	Applied	Applied	NNP
cord-265978-i0fu8e0p	65	30	Biosystems	Biosystems	NNP
cord-265978-i0fu8e0p	65	31	)	)	-RRB-
cord-265978-i0fu8e0p	65	32	.	.	.
cord-265978-i0fu8e0p	66	1	The	the	DT
cord-265978-i0fu8e0p	66	2	reaction	reaction	NN
cord-265978-i0fu8e0p	66	3	conditions	condition	NNS
cord-265978-i0fu8e0p	66	4	were	be	VBD
cord-265978-i0fu8e0p	66	5	first	first	RB
cord-265978-i0fu8e0p	66	6	optimized	optimize	VBN
cord-265978-i0fu8e0p	66	7	by	by	IN
cord-265978-i0fu8e0p	66	8	testing	test	VBG
cord-265978-i0fu8e0p	66	9	variable	variable	JJ
cord-265978-i0fu8e0p	66	10	concentrations	concentration	NNS
cord-265978-i0fu8e0p	66	11	of	of	IN
cord-265978-i0fu8e0p	66	12	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	66	13	and	and	CC
cord-265978-i0fu8e0p	66	14	internal	internal	JJ
cord-265978-i0fu8e0p	66	15	control	control	NN
cord-265978-i0fu8e0p	66	16	QPCR	QPCR	NNP
cord-265978-i0fu8e0p	66	17	primer	primer	NN
cord-265978-i0fu8e0p	66	18	sets	set	NNS
cord-265978-i0fu8e0p	66	19	;	;	:
cord-265978-i0fu8e0p	66	20	MgCl	MgCl	NNP
cord-265978-i0fu8e0p	66	21	2	2	CD
cord-265978-i0fu8e0p	66	22	concentrations	concentration	NNS
cord-265978-i0fu8e0p	66	23	;	;	:
cord-265978-i0fu8e0p	66	24	template	template	NN
cord-265978-i0fu8e0p	66	25	volumes	volume	NNS
cord-265978-i0fu8e0p	66	26	and	and	CC
cord-265978-i0fu8e0p	66	27	primer	primer	NN
cord-265978-i0fu8e0p	66	28	annealing	anneal	VBG
cord-265978-i0fu8e0p	66	29	Following	follow	VBG
cord-265978-i0fu8e0p	66	30	amplification	amplification	NN
cord-265978-i0fu8e0p	66	31	,	,	,
cord-265978-i0fu8e0p	66	32	a	a	DT
cord-265978-i0fu8e0p	66	33	melt	melt	NN
cord-265978-i0fu8e0p	66	34	curve	curve	NN
cord-265978-i0fu8e0p	66	35	analysis	analysis	NN
cord-265978-i0fu8e0p	66	36	was	be	VBD
cord-265978-i0fu8e0p	66	37	performed	perform	VBN
cord-265978-i0fu8e0p	66	38	to	to	TO
cord-265978-i0fu8e0p	66	39	verify	verify	VB
cord-265978-i0fu8e0p	66	40	the	the	DT
cord-265978-i0fu8e0p	66	41	specificity	specificity	NN
cord-265978-i0fu8e0p	66	42	of	of	IN
cord-265978-i0fu8e0p	66	43	the	the	DT
cord-265978-i0fu8e0p	66	44	amplified	amplify	VBN
cord-265978-i0fu8e0p	66	45	products	product	NNS
cord-265978-i0fu8e0p	66	46	by	by	IN
cord-265978-i0fu8e0p	66	47	their	-PRON-	PRP$
cord-265978-i0fu8e0p	66	48	specific	specific	JJ
cord-265978-i0fu8e0p	66	49	melting	melt	VBG
cord-265978-i0fu8e0p	66	50	temperatures	temperature	NNS
cord-265978-i0fu8e0p	66	51	(	(	-LRB-
cord-265978-i0fu8e0p	66	52	Tm	Tm	NNP
cord-265978-i0fu8e0p	66	53	)	)	-RRB-
cord-265978-i0fu8e0p	66	54	.	.	.
cord-265978-i0fu8e0p	67	1	Melting	melt	VBG
cord-265978-i0fu8e0p	67	2	curve	curve	NN
cord-265978-i0fu8e0p	67	3	analysis	analysis	NN
cord-265978-i0fu8e0p	67	4	consisted	consist	VBN
cord-265978-i0fu8e0p	67	5	of	of	IN
cord-265978-i0fu8e0p	67	6	a	a	DT
cord-265978-i0fu8e0p	67	7	denaturation	denaturation	NN
cord-265978-i0fu8e0p	67	8	step	step	NN
cord-265978-i0fu8e0p	67	9	at	at	IN
cord-265978-i0fu8e0p	67	10	95	95	CD
cord-265978-i0fu8e0p	67	11	C	c	NN
cord-265978-i0fu8e0p	67	12	for	for	IN
cord-265978-i0fu8e0p	67	13	15	15	CD
cord-265978-i0fu8e0p	67	14	s	s	NNS
cord-265978-i0fu8e0p	67	15	,	,	,
cord-265978-i0fu8e0p	67	16	decreased	decrease	VBD
cord-265978-i0fu8e0p	67	17	to	to	IN
cord-265978-i0fu8e0p	67	18	60	60	CD
cord-265978-i0fu8e0p	67	19	C	c	NN
cord-265978-i0fu8e0p	67	20	for	for	IN
cord-265978-i0fu8e0p	67	21	1	1	CD
cord-265978-i0fu8e0p	67	22	min	min	NN
cord-265978-i0fu8e0p	67	23	,	,	,
cord-265978-i0fu8e0p	67	24	and	and	CC
cord-265978-i0fu8e0p	67	25	followed	follow	VBN
cord-265978-i0fu8e0p	67	26	by	by	IN
cord-265978-i0fu8e0p	67	27	temperature	temperature	NN
cord-265978-i0fu8e0p	67	28	increase	increase	NN
cord-265978-i0fu8e0p	67	29	to	to	IN
cord-265978-i0fu8e0p	67	30	95	95	CD
cord-265978-i0fu8e0p	67	31	C	c	NN
cord-265978-i0fu8e0p	67	32	at	at	IN
cord-265978-i0fu8e0p	67	33	a	a	DT
cord-265978-i0fu8e0p	67	34	rate	rate	NN
cord-265978-i0fu8e0p	67	35	of	of	IN
cord-265978-i0fu8e0p	67	36	1	1	CD
cord-265978-i0fu8e0p	67	37	%	%	NN
cord-265978-i0fu8e0p	67	38	with	with	IN
cord-265978-i0fu8e0p	67	39	continuous	continuous	JJ
cord-265978-i0fu8e0p	67	40	reading	reading	NN
cord-265978-i0fu8e0p	67	41	of	of	IN
cord-265978-i0fu8e0p	67	42	fluorescence	fluorescence	NN
cord-265978-i0fu8e0p	67	43	.	.	.
cord-265978-i0fu8e0p	68	1	Data	data	NN
cord-265978-i0fu8e0p	68	2	acquisition	acquisition	NN
cord-265978-i0fu8e0p	68	3	and	and	CC
cord-265978-i0fu8e0p	68	4	analysis	analysis	NN
cord-265978-i0fu8e0p	68	5	of	of	IN
cord-265978-i0fu8e0p	68	6	the	the	DT
cord-265978-i0fu8e0p	68	7	results	result	NNS
cord-265978-i0fu8e0p	68	8	were	be	VBD
cord-265978-i0fu8e0p	68	9	performed	perform	VBN
cord-265978-i0fu8e0p	68	10	using	use	VBG
cord-265978-i0fu8e0p	68	11	the	the	DT
cord-265978-i0fu8e0p	68	12	7500	7500	CD
cord-265978-i0fu8e0p	68	13	System	System	NNP
cord-265978-i0fu8e0p	68	14	SDS	SDS	NNP
cord-265978-i0fu8e0p	68	15	Software	Software	NNP
cord-265978-i0fu8e0p	68	16	Version	Version	NNP
cord-265978-i0fu8e0p	68	17	2.0.1	2.0.1	CD
cord-265978-i0fu8e0p	68	18	(	(	-LRB-
cord-265978-i0fu8e0p	68	19	Applied	Applied	NNP
cord-265978-i0fu8e0p	68	20	Biosystems	Biosystems	NNP
cord-265978-i0fu8e0p	68	21	)	)	-RRB-
cord-265978-i0fu8e0p	68	22	.	.	.
cord-265978-i0fu8e0p	69	1	Each	each	DT
cord-265978-i0fu8e0p	69	2	fluorescent	fluorescent	JJ
cord-265978-i0fu8e0p	69	3	reporter	reporter	NN
cord-265978-i0fu8e0p	69	4	signal	signal	NN
cord-265978-i0fu8e0p	69	5	was	be	VBD
cord-265978-i0fu8e0p	69	6	measured	measure	VBN
cord-265978-i0fu8e0p	69	7	against	against	IN
cord-265978-i0fu8e0p	69	8	the	the	DT
cord-265978-i0fu8e0p	69	9	internal	internal	JJ
cord-265978-i0fu8e0p	69	10	reference	reference	NN
cord-265978-i0fu8e0p	69	11	dye	dye	NN
cord-265978-i0fu8e0p	69	12	(	(	-LRB-
cord-265978-i0fu8e0p	69	13	ROX	rox	NN
cord-265978-i0fu8e0p	69	14	)	)	-RRB-
cord-265978-i0fu8e0p	69	15	signal	signal	NN
cord-265978-i0fu8e0p	69	16	to	to	TO
cord-265978-i0fu8e0p	69	17	normalize	normalize	VB
cord-265978-i0fu8e0p	69	18	for	for	IN
cord-265978-i0fu8e0p	69	19	non	non	JJ
cord-265978-i0fu8e0p	69	20	-	-	JJ
cord-265978-i0fu8e0p	69	21	PCR	pcr	JJ
cord-265978-i0fu8e0p	69	22	related	related	JJ
cord-265978-i0fu8e0p	69	23	fluorescence	fluorescence	NN
cord-265978-i0fu8e0p	69	24	fluctuations	fluctuation	NNS
cord-265978-i0fu8e0p	69	25	between	between	IN
cord-265978-i0fu8e0p	69	26	wells	well	NNS
cord-265978-i0fu8e0p	69	27	.	.	.
cord-265978-i0fu8e0p	70	1	The	the	DT
cord-265978-i0fu8e0p	70	2	fluorescence	fluorescence	NN
cord-265978-i0fu8e0p	70	3	threshold	threshold	NN
cord-265978-i0fu8e0p	70	4	limit	limit	NN
cord-265978-i0fu8e0p	70	5	of	of	IN
cord-265978-i0fu8e0p	70	6	the	the	DT
cord-265978-i0fu8e0p	70	7	ABI	ABI	NNP
cord-265978-i0fu8e0p	70	8	Prism	Prism	NNP
cord-265978-i0fu8e0p	70	9	7500	7500	CD
cord-265978-i0fu8e0p	70	10	system	system	NN
cord-265978-i0fu8e0p	70	11	was	be	VBD
cord-265978-i0fu8e0p	70	12	set	set	VBN
cord-265978-i0fu8e0p	70	13	at	at	IN
cord-265978-i0fu8e0p	70	14	0.02	0.02	CD
cord-265978-i0fu8e0p	70	15	as	as	IN
cord-265978-i0fu8e0p	70	16	recommended	recommend	VBN
cord-265978-i0fu8e0p	70	17	by	by	IN
cord-265978-i0fu8e0p	70	18	the	the	DT
cord-265978-i0fu8e0p	70	19	manufacturer	manufacturer	NN
cord-265978-i0fu8e0p	70	20	.	.	.
cord-265978-i0fu8e0p	70	21	 	 	_SP
cord-265978-i0fu8e0p	71	1	A	a	DT
cord-265978-i0fu8e0p	71	2	fragment	fragment	NN
cord-265978-i0fu8e0p	71	3	of	of	IN
cord-265978-i0fu8e0p	71	4	1420	1420	CD
cord-265978-i0fu8e0p	71	5	bp	bp	NNP
cord-265978-i0fu8e0p	71	6	containing	contain	VBG
cord-265978-i0fu8e0p	71	7	the	the	DT
cord-265978-i0fu8e0p	71	8	entire	entire	JJ
cord-265978-i0fu8e0p	71	9	sequence	sequence	NN
cord-265978-i0fu8e0p	71	10	of	of	IN
cord-265978-i0fu8e0p	71	11	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	71	12	N	N	NNP
cord-265978-i0fu8e0p	71	13	gene	gene	NN
cord-265978-i0fu8e0p	71	14	was	be	VBD
cord-265978-i0fu8e0p	71	15	amplified	amplify	VBN
cord-265978-i0fu8e0p	71	16	using	use	VBG
cord-265978-i0fu8e0p	71	17	the	the	DT
cord-265978-i0fu8e0p	71	18	primer	primer	NN
cord-265978-i0fu8e0p	71	19	pair	pair	NN
cord-265978-i0fu8e0p	71	20	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	71	21	-	-	HYPH
cord-265978-i0fu8e0p	71	22	NF	NF	NNP
cord-265978-i0fu8e0p	71	23	/	/	SYM
cord-265978-i0fu8e0p	71	24	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	71	25	-	-	HYPH
cord-265978-i0fu8e0p	71	26	NR	NR	NNP
cord-265978-i0fu8e0p	71	27	(	(	-LRB-
cord-265978-i0fu8e0p	71	28	Table	table	NN
cord-265978-i0fu8e0p	71	29	2	2	CD
cord-265978-i0fu8e0p	71	30	)	)	-RRB-
cord-265978-i0fu8e0p	71	31	[	[	-LRB-
cord-265978-i0fu8e0p	71	32	14	14	CD
cord-265978-i0fu8e0p	71	33	]	]	-RRB-
cord-265978-i0fu8e0p	71	34	.	.	.
cord-265978-i0fu8e0p	72	1	The	the	DT
cord-265978-i0fu8e0p	72	2	RT	RT	NNP
cord-265978-i0fu8e0p	72	3	-	-	HYPH
cord-265978-i0fu8e0p	72	4	PCR	PCR	NNP
cord-265978-i0fu8e0p	72	5	product	product	NN
cord-265978-i0fu8e0p	72	6	was	be	VBD
cord-265978-i0fu8e0p	72	7	cloned	clone	VBN
cord-265978-i0fu8e0p	72	8	into	into	IN
cord-265978-i0fu8e0p	72	9	pCR	pCR	NNP
cord-265978-i0fu8e0p	72	10	Ò	Ò	NNP
cord-265978-i0fu8e0p	72	11	4-TOPO	4-topo	CD
cord-265978-i0fu8e0p	72	12	vector	vector	NN
cord-265978-i0fu8e0p	72	13	(	(	-LRB-
cord-265978-i0fu8e0p	72	14	Invitrogen	Invitrogen	NNP
cord-265978-i0fu8e0p	72	15	,	,	,
cord-265978-i0fu8e0p	72	16	Carlsbad	Carlsbad	NNP
cord-265978-i0fu8e0p	72	17	,	,	,
cord-265978-i0fu8e0p	72	18	CA	CA	NNP
cord-265978-i0fu8e0p	72	19	,	,	,
cord-265978-i0fu8e0p	72	20	USA	USA	NNP
cord-265978-i0fu8e0p	72	21	)	)	-RRB-
cord-265978-i0fu8e0p	72	22	according	accord	VBG
cord-265978-i0fu8e0p	72	23	to	to	IN
cord-265978-i0fu8e0p	72	24	the	the	DT
cord-265978-i0fu8e0p	72	25	manufacturer	manufacturer	NN
cord-265978-i0fu8e0p	72	26	guidelines	guideline	NNS
cord-265978-i0fu8e0p	72	27	.	.	.
cord-265978-i0fu8e0p	73	1	Plasmid	Plasmid	NNP
cord-265978-i0fu8e0p	73	2	DNA	DNA	NNP
cord-265978-i0fu8e0p	73	3	was	be	VBD
cord-265978-i0fu8e0p	73	4	recovered	recover	VBN
cord-265978-i0fu8e0p	73	5	from	from	IN
cord-265978-i0fu8e0p	73	6	the	the	DT
cord-265978-i0fu8e0p	73	7	transformed	transform	VBN
cord-265978-i0fu8e0p	73	8	Escherichia	Escherichia	NNP
cord-265978-i0fu8e0p	73	9	.	.	.
cord-265978-i0fu8e0p	74	1	coli	coli	NNS
cord-265978-i0fu8e0p	74	2	using	use	VBG
cord-265978-i0fu8e0p	74	3	the	the	DT
cord-265978-i0fu8e0p	74	4	QIAprep	QIAprep	NNP
cord-265978-i0fu8e0p	74	5	Spin	Spin	NNP
cord-265978-i0fu8e0p	74	6	Miniprep	Miniprep	NNP
cord-265978-i0fu8e0p	74	7	Kit	kit	NN
cord-265978-i0fu8e0p	74	8	(	(	-LRB-
cord-265978-i0fu8e0p	74	9	Qiagen	Qiagen	NNP
cord-265978-i0fu8e0p	74	10	)	)	-RRB-
cord-265978-i0fu8e0p	74	11	as	as	IN
cord-265978-i0fu8e0p	74	12	directed	direct	VBN
cord-265978-i0fu8e0p	74	13	by	by	IN
cord-265978-i0fu8e0p	74	14	the	the	DT
cord-265978-i0fu8e0p	74	15	manufacturer	manufacturer	NN
cord-265978-i0fu8e0p	74	16	and	and	CC
cord-265978-i0fu8e0p	74	17	quantified	quantify	VBN
cord-265978-i0fu8e0p	74	18	by	by	IN
cord-265978-i0fu8e0p	74	19	spectrophotometric	spectrophotometric	JJ
cord-265978-i0fu8e0p	74	20	analysis	analysis	NN
cord-265978-i0fu8e0p	74	21	.	.	.
cord-265978-i0fu8e0p	75	1	Appropriate	appropriate	JJ
cord-265978-i0fu8e0p	75	2	clones	clone	NNS
cord-265978-i0fu8e0p	75	3	were	be	VBD
cord-265978-i0fu8e0p	75	4	verified	verify	VBN
cord-265978-i0fu8e0p	75	5	by	by	IN
cord-265978-i0fu8e0p	75	6	restriction	restriction	NN
cord-265978-i0fu8e0p	75	7	endonuclease	endonuclease	NN
cord-265978-i0fu8e0p	75	8	digestion	digestion	NN
cord-265978-i0fu8e0p	75	9	and	and	CC
cord-265978-i0fu8e0p	75	10	DNA	dna	NN
cord-265978-i0fu8e0p	75	11	sequencing	sequencing	NN
cord-265978-i0fu8e0p	75	12	.	.	.
cord-265978-i0fu8e0p	76	1	Plasmid	Plasmid	NNP
cord-265978-i0fu8e0p	76	2	copy	copy	NN
cord-265978-i0fu8e0p	76	3	numbers	number	NNS
cord-265978-i0fu8e0p	76	4	were	be	VBD
cord-265978-i0fu8e0p	76	5	calculated	calculate	VBN
cord-265978-i0fu8e0p	76	6	using	use	VBG
cord-265978-i0fu8e0p	76	7	the	the	DT
cord-265978-i0fu8e0p	76	8	formula	formula	NN
cord-265978-i0fu8e0p	76	9	described	describe	VBN
cord-265978-i0fu8e0p	76	10	by	by	IN
cord-265978-i0fu8e0p	76	11	Ke	Ke	NNP
cord-265978-i0fu8e0p	76	12	et	et	NNP
cord-265978-i0fu8e0p	76	13	al	al	NNP
cord-265978-i0fu8e0p	76	14	.	.	.
cord-265978-i0fu8e0p	77	1	[	[	-LRB-
cord-265978-i0fu8e0p	77	2	15	15	CD
cord-265978-i0fu8e0p	77	3	]	]	-RRB-
cord-265978-i0fu8e0p	77	4	,	,	,
cord-265978-i0fu8e0p	77	5	.	.	.
cord-265978-i0fu8e0p	78	1	A	a	DT
cord-265978-i0fu8e0p	78	2	master	master	NN
cord-265978-i0fu8e0p	78	3	stock	stock	NN
cord-265978-i0fu8e0p	78	4	containing	contain	VBG
cord-265978-i0fu8e0p	78	5	5	5	CD
cord-265978-i0fu8e0p	78	6	Â	â	CD
cord-265978-i0fu8e0p	78	7	10	10	CD
cord-265978-i0fu8e0p	78	8	8	8	CD
cord-265978-i0fu8e0p	78	9	copies	copy	NNS
cord-265978-i0fu8e0p	78	10	/	/	SYM
cord-265978-i0fu8e0p	78	11	ml	ml	NNS
cord-265978-i0fu8e0p	78	12	was	be	VBD
cord-265978-i0fu8e0p	78	13	used	use	VBN
cord-265978-i0fu8e0p	78	14	for	for	IN
cord-265978-i0fu8e0p	78	15	preparation	preparation	NN
cord-265978-i0fu8e0p	78	16	of	of	IN
cord-265978-i0fu8e0p	78	17	10-fold	10-fold	CD
cord-265978-i0fu8e0p	78	18	dilution	dilution	NN
cord-265978-i0fu8e0p	78	19	series	series	NN
cord-265978-i0fu8e0p	78	20	to	to	TO
cord-265978-i0fu8e0p	78	21	generate	generate	VB
cord-265978-i0fu8e0p	78	22	the	the	DT
cord-265978-i0fu8e0p	78	23	standard	standard	JJ
cord-265978-i0fu8e0p	78	24	curve	curve	NN
cord-265978-i0fu8e0p	78	25	and	and	CC
cord-265978-i0fu8e0p	78	26	to	to	TO
cord-265978-i0fu8e0p	78	27	test	test	VB
cord-265978-i0fu8e0p	78	28	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	78	29	and	and	CC
cord-265978-i0fu8e0p	78	30	reproducibility	reproducibility	NN
cord-265978-i0fu8e0p	78	31	of	of	IN
cord-265978-i0fu8e0p	78	32	the	the	DT
cord-265978-i0fu8e0p	78	33	assay	assay	NN
cord-265978-i0fu8e0p	78	34	.	.	.
cord-265978-i0fu8e0p	79	1	The	the	DT
cord-265978-i0fu8e0p	79	2	specificity	specificity	NN
cord-265978-i0fu8e0p	79	3	of	of	IN
cord-265978-i0fu8e0p	79	4	the	the	DT
cord-265978-i0fu8e0p	79	5	developed	developed	JJ
cord-265978-i0fu8e0p	79	6	assay	assay	NN
cord-265978-i0fu8e0p	79	7	was	be	VBD
cord-265978-i0fu8e0p	79	8	assessed	assess	VBN
cord-265978-i0fu8e0p	79	9	against	against	IN
cord-265978-i0fu8e0p	79	10	viral	viral	JJ
cord-265978-i0fu8e0p	79	11	nucleic	nucleic	JJ
cord-265978-i0fu8e0p	79	12	acid	acid	NN
cord-265978-i0fu8e0p	79	13	extracted	extract	VBN
cord-265978-i0fu8e0p	79	14	from	from	IN
cord-265978-i0fu8e0p	79	15	a	a	DT
cord-265978-i0fu8e0p	79	16	range	range	NN
cord-265978-i0fu8e0p	79	17	of	of	IN
cord-265978-i0fu8e0p	79	18	animal	animal	NN
cord-265978-i0fu8e0p	79	19	viruses	virus	NNS
cord-265978-i0fu8e0p	79	20	of	of	IN
cord-265978-i0fu8e0p	79	21	clinical	clinical	JJ
cord-265978-i0fu8e0p	79	22	and	and	CC
cord-265978-i0fu8e0p	79	23	structural	structural	JJ
cord-265978-i0fu8e0p	79	24	relevance	relevance	NN
cord-265978-i0fu8e0p	79	25	to	to	IN
cord-265978-i0fu8e0p	79	26	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	79	27	(	(	-LRB-
cord-265978-i0fu8e0p	79	28	Table	table	NN
cord-265978-i0fu8e0p	79	29	1	1	CD
cord-265978-i0fu8e0p	79	30	)	)	-RRB-
cord-265978-i0fu8e0p	79	31	.	.	.
cord-265978-i0fu8e0p	80	1	The	the	DT
cord-265978-i0fu8e0p	80	2	analytical	analytical	JJ
cord-265978-i0fu8e0p	80	3	specificity	specificity	NN
cord-265978-i0fu8e0p	80	4	was	be	VBD
cord-265978-i0fu8e0p	80	5	further	further	RB
cord-265978-i0fu8e0p	80	6	established	establish	VBN
cord-265978-i0fu8e0p	80	7	by	by	IN
cord-265978-i0fu8e0p	80	8	performing	perform	VBG
cord-265978-i0fu8e0p	80	9	melt	melt	NN
cord-265978-i0fu8e0p	80	10	curve	curve	NN
cord-265978-i0fu8e0p	80	11	analysis	analysis	NN
cord-265978-i0fu8e0p	80	12	and	and	CC
cord-265978-i0fu8e0p	80	13	agarose	agarose	VBN
cord-265978-i0fu8e0p	80	14	gel	gel	NN
cord-265978-i0fu8e0p	80	15	electrophoresis	electrophoresis	NN
cord-265978-i0fu8e0p	80	16	on	on	IN
cord-265978-i0fu8e0p	80	17	the	the	DT
cord-265978-i0fu8e0p	80	18	final	final	JJ
cord-265978-i0fu8e0p	80	19	PCR	PCR	NNP
cord-265978-i0fu8e0p	80	20	product	product	NN
cord-265978-i0fu8e0p	80	21	.	.	.
cord-265978-i0fu8e0p	81	1	Negative	negative	JJ
cord-265978-i0fu8e0p	81	2	controls	control	NNS
cord-265978-i0fu8e0p	81	3	included	include	VBD
cord-265978-i0fu8e0p	81	4	fecal	fecal	JJ
cord-265978-i0fu8e0p	81	5	and	and	CC
cord-265978-i0fu8e0p	81	6	nasal	nasal	NN
cord-265978-i0fu8e0p	81	7	samples	sample	NNS
cord-265978-i0fu8e0p	81	8	collected	collect	VBN
cord-265978-i0fu8e0p	81	9	from	from	IN
cord-265978-i0fu8e0p	81	10	uninfected	uninfected	JJ
cord-265978-i0fu8e0p	81	11	calves	calf	NNS
cord-265978-i0fu8e0p	81	12	,	,	,
cord-265978-i0fu8e0p	81	13	unrelated	unrelated	JJ
cord-265978-i0fu8e0p	81	14	virus	virus	NN
cord-265978-i0fu8e0p	81	15	strains	strain	NNS
cord-265978-i0fu8e0p	81	16	like	like	IN
cord-265978-i0fu8e0p	81	17	NDV	NDV	NNP
cord-265978-i0fu8e0p	81	18	,	,	,
cord-265978-i0fu8e0p	81	19	ARV	ARV	NNP
cord-265978-i0fu8e0p	81	20	and	and	CC
cord-265978-i0fu8e0p	81	21	HPIV-3	HPIV-3	NNP
cord-265978-i0fu8e0p	81	22	and	and	CC
cord-265978-i0fu8e0p	81	23	MilliQ	milliq	NN
cord-265978-i0fu8e0p	81	24	water	water	NN
cord-265978-i0fu8e0p	81	25	.	.	.
cord-265978-i0fu8e0p	82	1	BCoV	bcov	NN
cord-265978-i0fu8e0p	82	2	reference	reference	NN
cord-265978-i0fu8e0p	82	3	strain	strain	NN
cord-265978-i0fu8e0p	82	4	was	be	VBD
cord-265978-i0fu8e0p	82	5	titrated	titrate	VBN
cord-265978-i0fu8e0p	82	6	on	on	IN
cord-265978-i0fu8e0p	82	7	MDBK	MDBK	NNP
cord-265978-i0fu8e0p	82	8	cells	cell	NNS
cord-265978-i0fu8e0p	82	9	using	use	VBG
cord-265978-i0fu8e0p	82	10	the	the	DT
cord-265978-i0fu8e0p	82	11	standard	standard	JJ
cord-265978-i0fu8e0p	82	12	methods	method	NNS
cord-265978-i0fu8e0p	82	13	[	[	-LRB-
cord-265978-i0fu8e0p	82	14	16	16	CD
cord-265978-i0fu8e0p	82	15	]	]	-RRB-
cord-265978-i0fu8e0p	82	16	.	.	.
cord-265978-i0fu8e0p	83	1	Ten	ten	CD
cord-265978-i0fu8e0p	83	2	-	-	HYPH
cord-265978-i0fu8e0p	83	3	fold	fold	JJ
cord-265978-i0fu8e0p	83	4	serial	serial	JJ
cord-265978-i0fu8e0p	83	5	dilutions	dilution	NNS
cord-265978-i0fu8e0p	83	6	of	of	IN
cord-265978-i0fu8e0p	83	7	the	the	DT
cord-265978-i0fu8e0p	83	8	original	original	JJ
cord-265978-i0fu8e0p	83	9	virus	virus	NN
cord-265978-i0fu8e0p	83	10	stock	stock	NN
cord-265978-i0fu8e0p	83	11	were	be	VBD
cord-265978-i0fu8e0p	83	12	prepared	prepare	VBN
cord-265978-i0fu8e0p	83	13	in	in	IN
cord-265978-i0fu8e0p	83	14	10	10	CD
cord-265978-i0fu8e0p	83	15	%	%	NN
cord-265978-i0fu8e0p	83	16	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	83	17	-	-	HYPH
cord-265978-i0fu8e0p	83	18	free	free	JJ
cord-265978-i0fu8e0p	83	19	nasal	nasal	NN
cord-265978-i0fu8e0p	83	20	/	/	SYM
cord-265978-i0fu8e0p	83	21	fecal	fecal	JJ
cord-265978-i0fu8e0p	83	22	suspension	suspension	NN
cord-265978-i0fu8e0p	83	23	.	.	.
cord-265978-i0fu8e0p	84	1	The	the	DT
cord-265978-i0fu8e0p	84	2	viral	viral	JJ
cord-265978-i0fu8e0p	84	3	RNA	RNA	NNP
cord-265978-i0fu8e0p	84	4	was	be	VBD
cord-265978-i0fu8e0p	84	5	extracted	extract	VBN
cord-265978-i0fu8e0p	84	6	from	from	IN
cord-265978-i0fu8e0p	84	7	each	each	DT
cord-265978-i0fu8e0p	84	8	dilution	dilution	NN
cord-265978-i0fu8e0p	84	9	and	and	CC
cord-265978-i0fu8e0p	84	10	was	be	VBD
cord-265978-i0fu8e0p	84	11	analyzed	analyze	VBN
cord-265978-i0fu8e0p	84	12	by	by	IN
cord-265978-i0fu8e0p	84	13	the	the	DT
cord-265978-i0fu8e0p	84	14	developed	developed	JJ
cord-265978-i0fu8e0p	84	15	two	two	CD
cord-265978-i0fu8e0p	84	16	-	-	HYPH
cord-265978-i0fu8e0p	84	17	step	step	NN
cord-265978-i0fu8e0p	84	18	real	real	JJ
cord-265978-i0fu8e0p	84	19	-	-	HYPH
cord-265978-i0fu8e0p	84	20	time	time	NN
cord-265978-i0fu8e0p	84	21	PCR	pcr	NN
cord-265978-i0fu8e0p	84	22	assay	assay	NN
cord-265978-i0fu8e0p	84	23	and	and	CC
cord-265978-i0fu8e0p	84	24	simultaneously	simultaneously	RB
cord-265978-i0fu8e0p	84	25	with	with	IN
cord-265978-i0fu8e0p	84	26	conventional	conventional	JJ
cord-265978-i0fu8e0p	84	27	PCR	PCR	NNP
cord-265978-i0fu8e0p	84	28	to	to	TO
cord-265978-i0fu8e0p	84	29	allow	allow	VB
cord-265978-i0fu8e0p	84	30	better	well	JJR
cord-265978-i0fu8e0p	84	31	comparison	comparison	NN
cord-265978-i0fu8e0p	84	32	of	of	IN
cord-265978-i0fu8e0p	84	33	the	the	DT
cord-265978-i0fu8e0p	84	34	analytical	analytical	JJ
cord-265978-i0fu8e0p	84	35	sensitivities	sensitivity	NNS
cord-265978-i0fu8e0p	84	36	.	.	.
cord-265978-i0fu8e0p	85	1	The	the	DT
cord-265978-i0fu8e0p	85	2	internal	internal	JJ
cord-265978-i0fu8e0p	85	3	BGAPDH	BGAPDH	NNP
cord-265978-i0fu8e0p	85	4	control	control	NN
cord-265978-i0fu8e0p	85	5	RNA	RNA	NNP
cord-265978-i0fu8e0p	85	6	was	be	VBD
cord-265978-i0fu8e0p	85	7	measured	measure	VBN
cord-265978-i0fu8e0p	85	8	in	in	IN
cord-265978-i0fu8e0p	85	9	all	all	DT
cord-265978-i0fu8e0p	85	10	dilutions	dilution	NNS
cord-265978-i0fu8e0p	85	11	for	for	IN
cord-265978-i0fu8e0p	85	12	quality	quality	NN
cord-265978-i0fu8e0p	85	13	management	management	NN
cord-265978-i0fu8e0p	85	14	.	.	.
cord-265978-i0fu8e0p	86	1	Additionally	additionally	RB
cord-265978-i0fu8e0p	86	2	,	,	,
cord-265978-i0fu8e0p	86	3	the	the	DT
cord-265978-i0fu8e0p	86	4	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	86	5	of	of	IN
cord-265978-i0fu8e0p	86	6	the	the	DT
cord-265978-i0fu8e0p	86	7	assay	assay	NN
cord-265978-i0fu8e0p	86	8	was	be	VBD
cord-265978-i0fu8e0p	86	9	determined	determine	VBN
cord-265978-i0fu8e0p	86	10	by	by	IN
cord-265978-i0fu8e0p	86	11	running	run	VBG
cord-265978-i0fu8e0p	86	12	10-fold	10-fold	JJ
cord-265978-i0fu8e0p	86	13	serial	serial	JJ
cord-265978-i0fu8e0p	86	14	dilutions	dilution	NNS
cord-265978-i0fu8e0p	86	15	of	of	IN
cord-265978-i0fu8e0p	86	16	the	the	DT
cord-265978-i0fu8e0p	86	17	plasmid	plasmid	NN
cord-265978-i0fu8e0p	86	18	standard	standard	NN
cord-265978-i0fu8e0p	86	19	in	in	IN
cord-265978-i0fu8e0p	86	20	duplicates	duplicate	NNS
cord-265978-i0fu8e0p	86	21	.	.	.
cord-265978-i0fu8e0p	87	1	The	the	DT
cord-265978-i0fu8e0p	87	2	lower	low	JJR
cord-265978-i0fu8e0p	87	3	limit	limit	NN
cord-265978-i0fu8e0p	87	4	of	of	IN
cord-265978-i0fu8e0p	87	5	detection	detection	NN
cord-265978-i0fu8e0p	87	6	was	be	VBD
cord-265978-i0fu8e0p	87	7	defined	define	VBN
cord-265978-i0fu8e0p	87	8	as	as	IN
cord-265978-i0fu8e0p	87	9	the	the	DT
cord-265978-i0fu8e0p	87	10	lowest	low	JJS
cord-265978-i0fu8e0p	87	11	dilution	dilution	NN
cord-265978-i0fu8e0p	87	12	in	in	IN
cord-265978-i0fu8e0p	87	13	the	the	DT
cord-265978-i0fu8e0p	87	14	10-fold	10-fold	JJ
cord-265978-i0fu8e0p	87	15	series	series	NN
cord-265978-i0fu8e0p	87	16	that	that	WDT
cord-265978-i0fu8e0p	87	17	amplified	amplify	VBD
cord-265978-i0fu8e0p	87	18	reliably	reliably	RB
cord-265978-i0fu8e0p	87	19	.	.	.
cord-265978-i0fu8e0p	88	1	To	to	TO
cord-265978-i0fu8e0p	88	2	evaluate	evaluate	VB
cord-265978-i0fu8e0p	88	3	reproducibility	reproducibility	NN
cord-265978-i0fu8e0p	88	4	of	of	IN
cord-265978-i0fu8e0p	88	5	the	the	DT
cord-265978-i0fu8e0p	88	6	assay	assay	NN
cord-265978-i0fu8e0p	88	7	,	,	,
cord-265978-i0fu8e0p	88	8	the	the	DT
cord-265978-i0fu8e0p	88	9	DNA	dna	NN
cord-265978-i0fu8e0p	88	10	standards	standard	NNS
cord-265978-i0fu8e0p	88	11	ranging	range	VBG
cord-265978-i0fu8e0p	88	12	from	from	IN
cord-265978-i0fu8e0p	88	13	10	10	CD
cord-265978-i0fu8e0p	88	14	8	8	CD
cord-265978-i0fu8e0p	88	15	to	to	TO
cord-265978-i0fu8e0p	88	16	10	10	CD
cord-265978-i0fu8e0p	88	17	3	3	CD
cord-265978-i0fu8e0p	88	18	copies	copy	NNS
cord-265978-i0fu8e0p	88	19	/	/	SYM
cord-265978-i0fu8e0p	88	20	reaction	reaction	NN
cord-265978-i0fu8e0p	88	21	were	be	VBD
cord-265978-i0fu8e0p	88	22	tested	test	VBN
cord-265978-i0fu8e0p	88	23	repeatedly	repeatedly	RB
cord-265978-i0fu8e0p	88	24	.	.	.
cord-265978-i0fu8e0p	89	1	Three	three	CD
cord-265978-i0fu8e0p	89	2	separate	separate	JJ
cord-265978-i0fu8e0p	89	3	dilution	dilution	NN
cord-265978-i0fu8e0p	89	4	series	series	NN
cord-265978-i0fu8e0p	89	5	were	be	VBD
cord-265978-i0fu8e0p	89	6	assayed	assay	VBN
cord-265978-i0fu8e0p	89	7	in	in	IN
cord-265978-i0fu8e0p	89	8	a	a	DT
cord-265978-i0fu8e0p	89	9	single	single	JJ
cord-265978-i0fu8e0p	89	10	run	run	NN
cord-265978-i0fu8e0p	89	11	for	for	IN
cord-265978-i0fu8e0p	89	12	evaluation	evaluation	NN
cord-265978-i0fu8e0p	89	13	of	of	IN
cord-265978-i0fu8e0p	89	14	the	the	DT
cord-265978-i0fu8e0p	89	15	intra	intra	JJ
cord-265978-i0fu8e0p	89	16	-	-	JJ
cord-265978-i0fu8e0p	89	17	assay	assay	JJ
cord-265978-i0fu8e0p	89	18	variations	variation	NNS
cord-265978-i0fu8e0p	89	19	.	.	.
cord-265978-i0fu8e0p	90	1	On	on	IN
cord-265978-i0fu8e0p	90	2	the	the	DT
cord-265978-i0fu8e0p	90	3	other	other	JJ
cord-265978-i0fu8e0p	90	4	hand	hand	NN
cord-265978-i0fu8e0p	90	5	,	,	,
cord-265978-i0fu8e0p	90	6	the	the	DT
cord-265978-i0fu8e0p	90	7	inter	inter	JJ
cord-265978-i0fu8e0p	90	8	-	-	JJ
cord-265978-i0fu8e0p	90	9	assay	assay	JJ
cord-265978-i0fu8e0p	90	10	variations	variation	NNS
cord-265978-i0fu8e0p	90	11	were	be	VBD
cord-265978-i0fu8e0p	90	12	measured	measure	VBN
cord-265978-i0fu8e0p	90	13	by	by	IN
cord-265978-i0fu8e0p	90	14	testing	test	VBG
cord-265978-i0fu8e0p	90	15	each	each	DT
cord-265978-i0fu8e0p	90	16	dilution	dilution	NN
cord-265978-i0fu8e0p	90	17	in	in	IN
cord-265978-i0fu8e0p	90	18	three	three	CD
cord-265978-i0fu8e0p	90	19	separate	separate	JJ
cord-265978-i0fu8e0p	90	20	runs	run	NNS
cord-265978-i0fu8e0p	90	21	.	.	.
cord-265978-i0fu8e0p	91	1	The	the	DT
cord-265978-i0fu8e0p	91	2	mean	mean	JJ
cord-265978-i0fu8e0p	91	3	,	,	,
cord-265978-i0fu8e0p	91	4	standard	standard	JJ
cord-265978-i0fu8e0p	91	5	deviation	deviation	NN
cord-265978-i0fu8e0p	91	6	(	(	-LRB-
cord-265978-i0fu8e0p	91	7	SD	sd	NN
cord-265978-i0fu8e0p	91	8	)	)	-RRB-
cord-265978-i0fu8e0p	91	9	and	and	CC
cord-265978-i0fu8e0p	91	10	coefficient	coefficient	NN
cord-265978-i0fu8e0p	91	11	of	of	IN
cord-265978-i0fu8e0p	91	12	variation	variation	NN
cord-265978-i0fu8e0p	91	13	(	(	-LRB-
cord-265978-i0fu8e0p	91	14	CV	CV	NNP
cord-265978-i0fu8e0p	91	15	)	)	-RRB-
cord-265978-i0fu8e0p	91	16	were	be	VBD
cord-265978-i0fu8e0p	91	17	calculated	calculate	VBN
cord-265978-i0fu8e0p	91	18	separately	separately	RB
cord-265978-i0fu8e0p	91	19	for	for	IN
cord-265978-i0fu8e0p	91	20	each	each	DT
cord-265978-i0fu8e0p	91	21	standard	standard	JJ
cord-265978-i0fu8e0p	91	22	DNA	dna	NN
cord-265978-i0fu8e0p	91	23	dilution	dilution	NN
cord-265978-i0fu8e0p	91	24	based	base	VBN
cord-265978-i0fu8e0p	91	25	on	on	IN
cord-265978-i0fu8e0p	91	26	their	-PRON-	PRP$
cord-265978-i0fu8e0p	91	27	C	c	NN
cord-265978-i0fu8e0p	91	28	T	t	NN
cord-265978-i0fu8e0p	91	29	values	value	NNS
cord-265978-i0fu8e0p	91	30	using	use	VBG
cord-265978-i0fu8e0p	91	31	Microsoft	Microsoft	NNP
cord-265978-i0fu8e0p	91	32	Excel	Excel	NNP
cord-265978-i0fu8e0p	91	33	software	software	NN
cord-265978-i0fu8e0p	91	34	.	.	.
cord-265978-i0fu8e0p	92	1	For	for	IN
cord-265978-i0fu8e0p	92	2	comparative	comparative	JJ
cord-265978-i0fu8e0p	92	3	purposes	purpose	NNS
cord-265978-i0fu8e0p	92	4	,	,	,
cord-265978-i0fu8e0p	92	5	gel	gel	NN
cord-265978-i0fu8e0p	92	6	-	-	HYPH
cord-265978-i0fu8e0p	92	7	based	base	VBN
cord-265978-i0fu8e0p	92	8	PCR	PCR	NNP
cord-265978-i0fu8e0p	92	9	was	be	VBD
cord-265978-i0fu8e0p	92	10	performed	perform	VBN
cord-265978-i0fu8e0p	92	11	on	on	IN
cord-265978-i0fu8e0p	92	12	the	the	DT
cord-265978-i0fu8e0p	92	13	cDNA	cdna	NN
cord-265978-i0fu8e0p	92	14	preparations	preparation	NNS
cord-265978-i0fu8e0p	92	15	of	of	IN
cord-265978-i0fu8e0p	92	16	the	the	DT
cord-265978-i0fu8e0p	92	17	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	92	18	10-fold	10-fold	CD
cord-265978-i0fu8e0p	92	19	dilution	dilution	NN
cord-265978-i0fu8e0p	92	20	series	series	NN
cord-265978-i0fu8e0p	92	21	(	(	-LRB-
cord-265978-i0fu8e0p	92	22	Section	Section	NNP
cord-265978-i0fu8e0p	92	23	2.7	2.7	CD
cord-265978-i0fu8e0p	92	24	)	)	-RRB-
cord-265978-i0fu8e0p	92	25	and	and	CC
cord-265978-i0fu8e0p	92	26	the	the	DT
cord-265978-i0fu8e0p	92	27	clinical	clinical	JJ
cord-265978-i0fu8e0p	92	28	samples	sample	NNS
cord-265978-i0fu8e0p	92	29	.	.	.
cord-265978-i0fu8e0p	93	1	The	the	DT
cord-265978-i0fu8e0p	93	2	PCR	PCR	NNP
cord-265978-i0fu8e0p	93	3	mixture	mixture	NN
cord-265978-i0fu8e0p	93	4	contained	contain	VBD
cord-265978-i0fu8e0p	93	5	12.5	12.5	CD
cord-265978-i0fu8e0p	93	6	ml	ml	NNS
cord-265978-i0fu8e0p	93	7	of	of	IN
cord-265978-i0fu8e0p	93	8	2Â	2â	CD
cord-265978-i0fu8e0p	93	9	Multiplex	Multiplex	NNP
cord-265978-i0fu8e0p	93	10	PCR	PCR	NNP
cord-265978-i0fu8e0p	93	11	Master	Master	NNP
cord-265978-i0fu8e0p	93	12	Mix	Mix	NNP
cord-265978-i0fu8e0p	93	13	(	(	-LRB-
cord-265978-i0fu8e0p	93	14	Qiagen	Qiagen	NNP
cord-265978-i0fu8e0p	93	15	)	)	-RRB-
cord-265978-i0fu8e0p	93	16	,	,	,
cord-265978-i0fu8e0p	93	17	0.5	0.5	CD
cord-265978-i0fu8e0p	93	18	ml	ml	NN
cord-265978-i0fu8e0p	93	19	(	(	-LRB-
cord-265978-i0fu8e0p	93	20	0.2	0.2	CD
cord-265978-i0fu8e0p	93	21	mM	mm	CD
cord-265978-i0fu8e0p	93	22	)	)	-RRB-
cord-265978-i0fu8e0p	93	23	of	of	IN
cord-265978-i0fu8e0p	93	24	each	each	DT
cord-265978-i0fu8e0p	93	25	primer	primer	NN
cord-265978-i0fu8e0p	93	26	(	(	-LRB-
cord-265978-i0fu8e0p	93	27	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	93	28	and	and	CC
cord-265978-i0fu8e0p	93	29	internal	internal	JJ
cord-265978-i0fu8e0p	93	30	control	control	NN
cord-265978-i0fu8e0p	93	31	qPCR	qpcr	NN
cord-265978-i0fu8e0p	93	32	primer	primer	NN
cord-265978-i0fu8e0p	93	33	sets	set	NNS
cord-265978-i0fu8e0p	93	34	)	)	-RRB-
cord-265978-i0fu8e0p	93	35	,	,	,
cord-265978-i0fu8e0p	93	36	2	2	CD
cord-265978-i0fu8e0p	93	37	ml	ml	NNS
cord-265978-i0fu8e0p	93	38	of	of	IN
cord-265978-i0fu8e0p	93	39	the	the	DT
cord-265978-i0fu8e0p	93	40	cDNA	cdna	NN
cord-265978-i0fu8e0p	93	41	,	,	,
cord-265978-i0fu8e0p	93	42	and	and	CC
cord-265978-i0fu8e0p	93	43	8.5	8.5	CD
cord-265978-i0fu8e0p	93	44	ml	ml	NN
cord-265978-i0fu8e0p	93	45	PCR	PCR	NNP
cord-265978-i0fu8e0p	93	46	grade	grade	NN
cord-265978-i0fu8e0p	93	47	water	water	NN
cord-265978-i0fu8e0p	93	48	.	.	.
cord-265978-i0fu8e0p	94	1	Amplification	amplification	NN
cord-265978-i0fu8e0p	94	2	of	of	IN
cord-265978-i0fu8e0p	94	3	target	target	NN
cord-265978-i0fu8e0p	94	4	sequences	sequence	NNS
cord-265978-i0fu8e0p	94	5	was	be	VBD
cord-265978-i0fu8e0p	94	6	conducted	conduct	VBN
cord-265978-i0fu8e0p	94	7	in	in	IN
cord-265978-i0fu8e0p	94	8	a	a	DT
cord-265978-i0fu8e0p	94	9	thermal	thermal	JJ
cord-265978-i0fu8e0p	94	10	cycler	cycler	NN
cord-265978-i0fu8e0p	94	11	(	(	-LRB-
cord-265978-i0fu8e0p	94	12	Applied	Applied	NNP
cord-265978-i0fu8e0p	94	13	Biosystem	Biosystem	NNP
cord-265978-i0fu8e0p	94	14	)	)	-RRB-
cord-265978-i0fu8e0p	94	15	using	use	VBG
cord-265978-i0fu8e0p	94	16	the	the	DT
cord-265978-i0fu8e0p	94	17	following	follow	VBG
cord-265978-i0fu8e0p	94	18	cycling	cycling	NN
cord-265978-i0fu8e0p	94	19	profile	profile	NN
cord-265978-i0fu8e0p	94	20	:	:	:
cord-265978-i0fu8e0p	94	21	initial	initial	JJ
cord-265978-i0fu8e0p	94	22	activation	activation	NN
cord-265978-i0fu8e0p	94	23	step	step	NN
cord-265978-i0fu8e0p	94	24	at	at	IN
cord-265978-i0fu8e0p	94	25	95	95	CD
cord-265978-i0fu8e0p	94	26	C	c	NN
cord-265978-i0fu8e0p	94	27	for	for	IN
cord-265978-i0fu8e0p	94	28	15	15	CD
cord-265978-i0fu8e0p	94	29	min	min	NN
cord-265978-i0fu8e0p	94	30	;	;	:
cord-265978-i0fu8e0p	94	31	35	35	CD
cord-265978-i0fu8e0p	94	32	cycles	cycle	NNS
cord-265978-i0fu8e0p	94	33	of	of	IN
cord-265978-i0fu8e0p	94	34	denaturation	denaturation	NN
cord-265978-i0fu8e0p	94	35	at	at	IN
cord-265978-i0fu8e0p	94	36	94	94	CD
cord-265978-i0fu8e0p	94	37	C	c	NN
cord-265978-i0fu8e0p	94	38	for	for	IN
cord-265978-i0fu8e0p	94	39	30	30	CD
cord-265978-i0fu8e0p	94	40	s	s	NN
cord-265978-i0fu8e0p	94	41	,	,	,
cord-265978-i0fu8e0p	94	42	primer	primer	NN
cord-265978-i0fu8e0p	94	43	annealing	annealing	NN
cord-265978-i0fu8e0p	94	44	at	at	IN
cord-265978-i0fu8e0p	94	45	57	57	CD
cord-265978-i0fu8e0p	94	46	C	c	NN
cord-265978-i0fu8e0p	94	47	Table	table	NN
cord-265978-i0fu8e0p	94	48	3	3	CD
cord-265978-i0fu8e0p	94	49	Reproducibility	Reproducibility	NNP
cord-265978-i0fu8e0p	94	50	of	of	IN
cord-265978-i0fu8e0p	94	51	the	the	DT
cord-265978-i0fu8e0p	94	52	BCV	BCV	NNP
cord-265978-i0fu8e0p	94	53	real	real	JJ
cord-265978-i0fu8e0p	94	54	-	-	HYPH
cord-265978-i0fu8e0p	94	55	time	time	NN
cord-265978-i0fu8e0p	94	56	RT	RT	NNP
cord-265978-i0fu8e0p	94	57	-	-	HYPH
cord-265978-i0fu8e0p	94	58	PCR	PCR	NNP
cord-265978-i0fu8e0p	94	59	assay	assay	NN
cord-265978-i0fu8e0p	94	60	.	.	.
cord-265978-i0fu8e0p	95	1	Cycle	cycle	NN
cord-265978-i0fu8e0p	95	2	of	of	IN
cord-265978-i0fu8e0p	95	3	threshold	threshold	NN
cord-265978-i0fu8e0p	95	4	(	(	-LRB-
cord-265978-i0fu8e0p	95	5	C	C	NNP
cord-265978-i0fu8e0p	95	6	T	T	NNP
cord-265978-i0fu8e0p	95	7	)	)	-RRB-
cord-265978-i0fu8e0p	95	8	for	for	IN
cord-265978-i0fu8e0p	95	9	DNA	dna	NN
cord-265978-i0fu8e0p	95	10	standards	standard	NNS
cord-265978-i0fu8e0p	95	11	(	(	-LRB-
cord-265978-i0fu8e0p	95	12	copies	copy	NNS
cord-265978-i0fu8e0p	95	13	/	/	SYM
cord-265978-i0fu8e0p	95	14	reaction	reaction	NN
cord-265978-i0fu8e0p	95	15	)	)	-RRB-
cord-265978-i0fu8e0p	96	1	Intra	intra	JJ
cord-265978-i0fu8e0p	96	2	-	-	JJ
cord-265978-i0fu8e0p	96	3	assay	assay	JJ
cord-265978-i0fu8e0p	96	4	variation	variation	NN
cord-265978-i0fu8e0p	96	5	for	for	IN
cord-265978-i0fu8e0p	96	6	90	90	CD
cord-265978-i0fu8e0p	96	7	s	s	NN
cord-265978-i0fu8e0p	96	8	and	and	CC
cord-265978-i0fu8e0p	96	9	extension	extension	NN
cord-265978-i0fu8e0p	96	10	at	at	IN
cord-265978-i0fu8e0p	96	11	72	72	CD
cord-265978-i0fu8e0p	96	12	C	c	NN
cord-265978-i0fu8e0p	96	13	for	for	IN
cord-265978-i0fu8e0p	96	14	90	90	CD
cord-265978-i0fu8e0p	96	15	s	s	NNS
cord-265978-i0fu8e0p	96	16	;	;	:
cord-265978-i0fu8e0p	96	17	and	and	CC
cord-265978-i0fu8e0p	96	18	final	final	JJ
cord-265978-i0fu8e0p	96	19	extension	extension	NN
cord-265978-i0fu8e0p	96	20	step	step	NN
cord-265978-i0fu8e0p	96	21	at	at	IN
cord-265978-i0fu8e0p	96	22	72	72	CD
cord-265978-i0fu8e0p	96	23	C	c	NN
cord-265978-i0fu8e0p	96	24	for	for	IN
cord-265978-i0fu8e0p	96	25	10	10	CD
cord-265978-i0fu8e0p	96	26	min	min	NN
cord-265978-i0fu8e0p	96	27	.	.	.
cord-265978-i0fu8e0p	97	1	PCR	PCR	NNP
cord-265978-i0fu8e0p	97	2	products	product	NNS
cord-265978-i0fu8e0p	97	3	were	be	VBD
cord-265978-i0fu8e0p	97	4	separated	separate	VBN
cord-265978-i0fu8e0p	97	5	in	in	IN
cord-265978-i0fu8e0p	97	6	1.5	1.5	CD
cord-265978-i0fu8e0p	97	7	%	%	NN
cord-265978-i0fu8e0p	97	8	agarose	agarose	NN
cord-265978-i0fu8e0p	97	9	gel	gel	NN
cord-265978-i0fu8e0p	97	10	containing	contain	VBG
cord-265978-i0fu8e0p	97	11	0.5	0.5	CD
cord-265978-i0fu8e0p	97	12	mg	mg	NNP
cord-265978-i0fu8e0p	97	13	/	/	SYM
cord-265978-i0fu8e0p	97	14	ml	ml	NN
cord-265978-i0fu8e0p	97	15	ethidium	ethidium	NN
cord-265978-i0fu8e0p	97	16	bromide	bromide	NN
cord-265978-i0fu8e0p	97	17	.	.	.
cord-265978-i0fu8e0p	98	1	The	the	DT
cord-265978-i0fu8e0p	98	2	specific	specific	JJ
cord-265978-i0fu8e0p	98	3	bands	band	NNS
cord-265978-i0fu8e0p	98	4	were	be	VBD
cord-265978-i0fu8e0p	98	5	identified	identify	VBN
cord-265978-i0fu8e0p	98	6	in	in	IN
cord-265978-i0fu8e0p	98	7	comparison	comparison	NN
cord-265978-i0fu8e0p	98	8	with	with	IN
cord-265978-i0fu8e0p	98	9	100	100	CD
cord-265978-i0fu8e0p	98	10	bp	bp	NNP
cord-265978-i0fu8e0p	98	11	DNA	DNA	NNP
cord-265978-i0fu8e0p	98	12	ladder	ladder	NN
cord-265978-i0fu8e0p	98	13	(	(	-LRB-
cord-265978-i0fu8e0p	98	14	ABgene	ABgene	NNP
cord-265978-i0fu8e0p	98	15	,	,	,
cord-265978-i0fu8e0p	98	16	Epsom	Epsom	NNP
cord-265978-i0fu8e0p	98	17	,	,	,
cord-265978-i0fu8e0p	98	18	Surrey	Surrey	NNP
cord-265978-i0fu8e0p	98	19	,	,	,
cord-265978-i0fu8e0p	98	20	UK	UK	NNP
cord-265978-i0fu8e0p	98	21	)	)	-RRB-
cord-265978-i0fu8e0p	98	22	and	and	CC
cord-265978-i0fu8e0p	98	23	documented	document	VBD
cord-265978-i0fu8e0p	98	24	using	use	VBG
cord-265978-i0fu8e0p	98	25	an	an	DT
cord-265978-i0fu8e0p	98	26	image	image	NN
cord-265978-i0fu8e0p	98	27	analysis	analysis	NN
cord-265978-i0fu8e0p	98	28	system	system	NN
cord-265978-i0fu8e0p	98	29	(	(	-LRB-
cord-265978-i0fu8e0p	98	30	IMAGO	IMAGO	NNP
cord-265978-i0fu8e0p	98	31	Compact	Compact	NNP
cord-265978-i0fu8e0p	98	32	Imaging	Imaging	NNP
cord-265978-i0fu8e0p	98	33	System	System	NNP
cord-265978-i0fu8e0p	98	34	,	,	,
cord-265978-i0fu8e0p	98	35	B&L	B&L	NNP
cord-265978-i0fu8e0p	98	36	,	,	,
cord-265978-i0fu8e0p	98	37	USA	USA	NNP
cord-265978-i0fu8e0p	98	38	)	)	-RRB-
cord-265978-i0fu8e0p	98	39	.	.	.
cord-265978-i0fu8e0p	99	1	In	in	IN
cord-265978-i0fu8e0p	99	2	order	order	NN
cord-265978-i0fu8e0p	99	3	to	to	TO
cord-265978-i0fu8e0p	99	4	determine	determine	VB
cord-265978-i0fu8e0p	99	5	the	the	DT
cord-265978-i0fu8e0p	99	6	optimal	optimal	JJ
cord-265978-i0fu8e0p	99	7	conditions	condition	NNS
cord-265978-i0fu8e0p	99	8	for	for	IN
cord-265978-i0fu8e0p	99	9	developing	develop	VBG
cord-265978-i0fu8e0p	99	10	a	a	DT
cord-265978-i0fu8e0p	99	11	robust	robust	JJ
cord-265978-i0fu8e0p	99	12	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	99	13	Green	Green	NNP
cord-265978-i0fu8e0p	99	14	I	-PRON-	PRP
cord-265978-i0fu8e0p	99	15	based	base	VBD
cord-265978-i0fu8e0p	99	16	real	real	JJ
cord-265978-i0fu8e0p	99	17	-	-	HYPH
cord-265978-i0fu8e0p	99	18	time	time	NN
cord-265978-i0fu8e0p	99	19	qPCR	qpcr	NN
cord-265978-i0fu8e0p	99	20	assay	assay	NN
cord-265978-i0fu8e0p	99	21	that	that	WDT
cord-265978-i0fu8e0p	99	22	detects	detect	VBZ
cord-265978-i0fu8e0p	99	23	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	99	24	and	and	CC
cord-265978-i0fu8e0p	99	25	internal	internal	JJ
cord-265978-i0fu8e0p	99	26	control	control	NN
cord-265978-i0fu8e0p	99	27	RNA	RNA	NNP
cord-265978-i0fu8e0p	99	28	simultaneously	simultaneously	RB
cord-265978-i0fu8e0p	99	29	,	,	,
cord-265978-i0fu8e0p	99	30	different	different	JJ
cord-265978-i0fu8e0p	99	31	variables	variable	NNS
cord-265978-i0fu8e0p	99	32	of	of	IN
cord-265978-i0fu8e0p	99	33	the	the	DT
cord-265978-i0fu8e0p	99	34	reaction	reaction	NN
cord-265978-i0fu8e0p	99	35	were	be	VBD
cord-265978-i0fu8e0p	99	36	assessed	assess	VBN
cord-265978-i0fu8e0p	99	37	.	.	.
cord-265978-i0fu8e0p	100	1	Such	such	JJ
cord-265978-i0fu8e0p	100	2	parameters	parameter	NNS
cord-265978-i0fu8e0p	100	3	included	include	VBD
cord-265978-i0fu8e0p	100	4	:	:	:
cord-265978-i0fu8e0p	100	5	magnesium	magnesium	NN
cord-265978-i0fu8e0p	100	6	chloride	chloride	NN
cord-265978-i0fu8e0p	100	7	concentration	concentration	NN
cord-265978-i0fu8e0p	100	8	(	(	-LRB-
cord-265978-i0fu8e0p	100	9	2.5e4	2.5e4	CD
cord-265978-i0fu8e0p	100	10	mM	mm	NN
cord-265978-i0fu8e0p	100	11	)	)	-RRB-
cord-265978-i0fu8e0p	100	12	;	;	:
cord-265978-i0fu8e0p	100	13	concentration	concentration	NN
cord-265978-i0fu8e0p	100	14	of	of	IN
cord-265978-i0fu8e0p	100	15	the	the	DT
cord-265978-i0fu8e0p	100	16	two	two	CD
cord-265978-i0fu8e0p	100	17	sets	set	NNS
cord-265978-i0fu8e0p	100	18	of	of	IN
cord-265978-i0fu8e0p	100	19	primers	primer	NNS
cord-265978-i0fu8e0p	100	20	(	(	-LRB-
cord-265978-i0fu8e0p	100	21	0.2e1	0.2e1	CD
cord-265978-i0fu8e0p	100	22	mM	mm	CD
cord-265978-i0fu8e0p	100	23	)	)	-RRB-
cord-265978-i0fu8e0p	100	24	;	;	:
cord-265978-i0fu8e0p	100	25	template	template	NN
cord-265978-i0fu8e0p	100	26	volume	volume	NN
cord-265978-i0fu8e0p	100	27	(	(	-LRB-
cord-265978-i0fu8e0p	100	28	1e5	1e5	CD
cord-265978-i0fu8e0p	100	29	ml	ml	NN
cord-265978-i0fu8e0p	100	30	)	)	-RRB-
cord-265978-i0fu8e0p	100	31	;	;	:
cord-265978-i0fu8e0p	100	32	and	and	CC
cord-265978-i0fu8e0p	100	33	annealing	anneal	VBG
cord-265978-i0fu8e0p	100	34	temperature	temperature	NN
cord-265978-i0fu8e0p	100	35	(	(	-LRB-
cord-265978-i0fu8e0p	100	36	55e60	55e60	CD
cord-265978-i0fu8e0p	100	37	C	C	NNP
cord-265978-i0fu8e0p	100	38	)	)	-RRB-
cord-265978-i0fu8e0p	100	39	.	.	.
cord-265978-i0fu8e0p	101	1	Criteria	criterion	NNS
cord-265978-i0fu8e0p	101	2	for	for	IN
cord-265978-i0fu8e0p	101	3	selection	selection	NN
cord-265978-i0fu8e0p	101	4	of	of	IN
cord-265978-i0fu8e0p	101	5	the	the	DT
cord-265978-i0fu8e0p	101	6	proper	proper	JJ
cord-265978-i0fu8e0p	101	7	measures	measure	NNS
cord-265978-i0fu8e0p	101	8	were	be	VBD
cord-265978-i0fu8e0p	101	9	the	the	DT
cord-265978-i0fu8e0p	101	10	intensity	intensity	NN
cord-265978-i0fu8e0p	101	11	of	of	IN
cord-265978-i0fu8e0p	101	12	fluorescence	fluorescence	NN
cord-265978-i0fu8e0p	101	13	and	and	CC
cord-265978-i0fu8e0p	101	14	the	the	DT
cord-265978-i0fu8e0p	101	15	number	number	NN
cord-265978-i0fu8e0p	101	16	of	of	IN
cord-265978-i0fu8e0p	101	17	cycles	cycle	NNS
cord-265978-i0fu8e0p	101	18	required	require	VBN
cord-265978-i0fu8e0p	101	19	to	to	TO
cord-265978-i0fu8e0p	101	20	develop	develop	VB
cord-265978-i0fu8e0p	101	21	a	a	DT
cord-265978-i0fu8e0p	101	22	detectable	detectable	JJ
cord-265978-i0fu8e0p	101	23	signal	signal	NN
cord-265978-i0fu8e0p	101	24	(	(	-LRB-
cord-265978-i0fu8e0p	101	25	C	c	NN
cord-265978-i0fu8e0p	101	26	T	t	NN
cord-265978-i0fu8e0p	101	27	values	value	NNS
cord-265978-i0fu8e0p	101	28	)	)	-RRB-
cord-265978-i0fu8e0p	101	29	.	.	.
cord-265978-i0fu8e0p	102	1	Ideal	ideal	JJ
cord-265978-i0fu8e0p	102	2	performance	performance	NN
cord-265978-i0fu8e0p	102	3	of	of	IN
cord-265978-i0fu8e0p	102	4	the	the	DT
cord-265978-i0fu8e0p	102	5	assay	assay	NN
cord-265978-i0fu8e0p	102	6	was	be	VBD
cord-265978-i0fu8e0p	102	7	achieved	achieve	VBN
cord-265978-i0fu8e0p	102	8	by	by	IN
cord-265978-i0fu8e0p	102	9	the	the	DT
cord-265978-i0fu8e0p	102	10	use	use	NN
cord-265978-i0fu8e0p	102	11	of	of	IN
cord-265978-i0fu8e0p	102	12	Quantitect	Quantitect	NNP
cord-265978-i0fu8e0p	102	13	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	102	14	Green	Green	NNP
cord-265978-i0fu8e0p	102	15	PCR	PCR	NNP
cord-265978-i0fu8e0p	102	16	Master	Master	NNP
cord-265978-i0fu8e0p	102	17	Mix	Mix	NNP
cord-265978-i0fu8e0p	102	18	(	(	-LRB-
cord-265978-i0fu8e0p	102	19	Qiagen	Qiagen	NNP
cord-265978-i0fu8e0p	102	20	)	)	-RRB-
cord-265978-i0fu8e0p	102	21	along	along	IN
cord-265978-i0fu8e0p	102	22	with	with	IN
cord-265978-i0fu8e0p	102	23	the	the	DT
cord-265978-i0fu8e0p	102	24	optimized	optimize	VBN
cord-265978-i0fu8e0p	102	25	concentrations	concentration	NNS
cord-265978-i0fu8e0p	102	26	of	of	IN
cord-265978-i0fu8e0p	102	27	primers	primer	NNS
cord-265978-i0fu8e0p	102	28	and	and	CC
cord-265978-i0fu8e0p	102	29	magnesium	magnesium	NN
cord-265978-i0fu8e0p	102	30	chloride	chloride	NN
cord-265978-i0fu8e0p	102	31	as	as	IN
cord-265978-i0fu8e0p	102	32	mentioned	mention	VBN
cord-265978-i0fu8e0p	102	33	in	in	IN
cord-265978-i0fu8e0p	102	34	Materials	material	NNS
cord-265978-i0fu8e0p	102	35	and	and	CC
cord-265978-i0fu8e0p	102	36	methods	method	NNS
cord-265978-i0fu8e0p	102	37	Section	section	NN
cord-265978-i0fu8e0p	102	38	2.4	2.4	CD
cord-265978-i0fu8e0p	102	39	.	.	.
cord-265978-i0fu8e0p	103	1	Specific	specific	JJ
cord-265978-i0fu8e0p	103	2	internal	internal	JJ
cord-265978-i0fu8e0p	103	3	control	control	NN
cord-265978-i0fu8e0p	103	4	primers	primer	NNS
cord-265978-i0fu8e0p	103	5	that	that	WDT
cord-265978-i0fu8e0p	103	6	target	target	VBP
cord-265978-i0fu8e0p	103	7	unique	unique	JJ
cord-265978-i0fu8e0p	103	8	sequences	sequence	NNS
cord-265978-i0fu8e0p	103	9	of	of	IN
cord-265978-i0fu8e0p	103	10	the	the	DT
cord-265978-i0fu8e0p	103	11	bovine	bovine	JJ
cord-265978-i0fu8e0p	103	12	GAPDH	GAPDH	NNP
cord-265978-i0fu8e0p	103	13	mRNA	mrna	NN
cord-265978-i0fu8e0p	103	14	were	be	VBD
cord-265978-i0fu8e0p	103	15	designed	design	VBN
cord-265978-i0fu8e0p	103	16	to	to	TO
cord-265978-i0fu8e0p	103	17	validate	validate	VB
cord-265978-i0fu8e0p	103	18	all	all	PDT
cord-265978-i0fu8e0p	103	19	the	the	DT
cord-265978-i0fu8e0p	103	20	reaction	reaction	NN
cord-265978-i0fu8e0p	103	21	steps	step	NNS
cord-265978-i0fu8e0p	103	22	from	from	IN
cord-265978-i0fu8e0p	103	23	RNA	RNA	NNP
cord-265978-i0fu8e0p	103	24	extraction	extraction	NN
cord-265978-i0fu8e0p	103	25	to	to	TO
cord-265978-i0fu8e0p	103	26	qPCR	qpcr	VB
cord-265978-i0fu8e0p	103	27	amplification	amplification	NN
cord-265978-i0fu8e0p	103	28	/	/	SYM
cord-265978-i0fu8e0p	103	29	detection	detection	NN
cord-265978-i0fu8e0p	103	30	(	(	-LRB-
cord-265978-i0fu8e0p	103	31	Table	Table	NNP
cord-265978-i0fu8e0p	103	32	2	2	CD
cord-265978-i0fu8e0p	103	33	)	)	-RRB-
cord-265978-i0fu8e0p	103	34	.	.	.
cord-265978-i0fu8e0p	104	1	These	these	DT
cord-265978-i0fu8e0p	104	2	primers	primer	NNS
cord-265978-i0fu8e0p	104	3	also	also	RB
cord-265978-i0fu8e0p	104	4	confirm	confirm	VBP
cord-265978-i0fu8e0p	104	5	that	that	IN
cord-265978-i0fu8e0p	104	6	the	the	DT
cord-265978-i0fu8e0p	104	7	reaction	reaction	NN
cord-265978-i0fu8e0p	104	8	was	be	VBD
cord-265978-i0fu8e0p	104	9	performed	perform	VBN
cord-265978-i0fu8e0p	104	10	correctly	correctly	RB
cord-265978-i0fu8e0p	104	11	and	and	CC
cord-265978-i0fu8e0p	104	12	that	that	IN
cord-265978-i0fu8e0p	104	13	no	no	DT
cord-265978-i0fu8e0p	104	14	excess	excess	NN
cord-265978-i0fu8e0p	104	15	of	of	IN
cord-265978-i0fu8e0p	104	16	inhibitors	inhibitor	NNS
cord-265978-i0fu8e0p	104	17	was	be	VBD
cord-265978-i0fu8e0p	104	18	present	present	JJ
cord-265978-i0fu8e0p	104	19	in	in	IN
cord-265978-i0fu8e0p	104	20	the	the	DT
cord-265978-i0fu8e0p	104	21	extracted	extract	VBN
cord-265978-i0fu8e0p	104	22	sample	sample	NN
cord-265978-i0fu8e0p	104	23	thus	thus	RB
cord-265978-i0fu8e0p	104	24	excluding	exclude	VBG
cord-265978-i0fu8e0p	104	25	false	false	JJ
cord-265978-i0fu8e0p	104	26	-	-	HYPH
cord-265978-i0fu8e0p	104	27	negatives	negative	NNS
cord-265978-i0fu8e0p	104	28	.	.	.
cord-265978-i0fu8e0p	105	1	Detection	detection	NN
cord-265978-i0fu8e0p	105	2	of	of	IN
cord-265978-i0fu8e0p	105	3	internal	internal	JJ
cord-265978-i0fu8e0p	105	4	control	control	NN
cord-265978-i0fu8e0p	105	5	RNA	RNA	NNP
cord-265978-i0fu8e0p	105	6	was	be	VBD
cord-265978-i0fu8e0p	105	7	performed	perform	VBN
cord-265978-i0fu8e0p	105	8	in	in	IN
cord-265978-i0fu8e0p	105	9	all	all	DT
cord-265978-i0fu8e0p	105	10	experiments	experiment	NNS
cord-265978-i0fu8e0p	105	11	,	,	,
cord-265978-i0fu8e0p	105	12	mostly	mostly	RB
cord-265978-i0fu8e0p	105	13	by	by	IN
cord-265978-i0fu8e0p	105	14	the	the	DT
cord-265978-i0fu8e0p	105	15	use	use	NN
cord-265978-i0fu8e0p	105	16	of	of	IN
cord-265978-i0fu8e0p	105	17	nasal	nasal	NN
cord-265978-i0fu8e0p	105	18	/	/	SYM
cord-265978-i0fu8e0p	105	19	fecal	fecal	JJ
cord-265978-i0fu8e0p	105	20	suspension	suspension	NN
cord-265978-i0fu8e0p	105	21	prepared	prepare	VBN
cord-265978-i0fu8e0p	105	22	from	from	IN
cord-265978-i0fu8e0p	105	23	non	non	JJ
cord-265978-i0fu8e0p	105	24	-	-	JJ
cord-265978-i0fu8e0p	105	25	infected	infected	JJ
cord-265978-i0fu8e0p	105	26	calves	calf	NNS
cord-265978-i0fu8e0p	105	27	as	as	IN
cord-265978-i0fu8e0p	105	28	a	a	DT
cord-265978-i0fu8e0p	105	29	matrix	matrix	NN
cord-265978-i0fu8e0p	105	30	.	.	.
cord-265978-i0fu8e0p	106	1	Identification	identification	NN
cord-265978-i0fu8e0p	106	2	of	of	IN
cord-265978-i0fu8e0p	106	3	the	the	DT
cord-265978-i0fu8e0p	106	4	internal	internal	JJ
cord-265978-i0fu8e0p	106	5	control	control	NN
cord-265978-i0fu8e0p	106	6	signal	signal	NN
cord-265978-i0fu8e0p	106	7	was	be	VBD
cord-265978-i0fu8e0p	106	8	achieved	achieve	VBN
cord-265978-i0fu8e0p	106	9	by	by	IN
cord-265978-i0fu8e0p	106	10	melting	melt	VBG
cord-265978-i0fu8e0p	106	11	curve	curve	NN
cord-265978-i0fu8e0p	106	12	analysis	analysis	NN
cord-265978-i0fu8e0p	106	13	,	,	,
cord-265978-i0fu8e0p	106	14	where	where	WRB
cord-265978-i0fu8e0p	106	15	a	a	DT
cord-265978-i0fu8e0p	106	16	distinct	distinct	JJ
cord-265978-i0fu8e0p	106	17	melting	melting	NN
cord-265978-i0fu8e0p	106	18	temperature	temperature	NN
cord-265978-i0fu8e0p	106	19	(	(	-LRB-
cord-265978-i0fu8e0p	106	20	Tm	Tm	NNP
cord-265978-i0fu8e0p	106	21	)	)	-RRB-
cord-265978-i0fu8e0p	106	22	of	of	IN
cord-265978-i0fu8e0p	106	23	82.54	82.54	CD
cord-265978-i0fu8e0p	106	24	AE	AE	NNP
cord-265978-i0fu8e0p	106	25	0.32	0.32	CD
cord-265978-i0fu8e0p	106	26	was	be	VBD
cord-265978-i0fu8e0p	106	27	obtained	obtain	VBN
cord-265978-i0fu8e0p	106	28	.	.	.
cord-265978-i0fu8e0p	107	1	The	the	DT
cord-265978-i0fu8e0p	107	2	entire	entire	JJ
cord-265978-i0fu8e0p	107	3	N	n	CD
cord-265978-i0fu8e0p	107	4	gene	gene	NN
cord-265978-i0fu8e0p	107	5	of	of	IN
cord-265978-i0fu8e0p	107	6	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	107	7	was	be	VBD
cord-265978-i0fu8e0p	107	8	cloned	clone	VBN
cord-265978-i0fu8e0p	107	9	in	in	IN
cord-265978-i0fu8e0p	107	10	pCR	pCR	NNP
cord-265978-i0fu8e0p	107	11	Ò	Ò	NNP
cord-265978-i0fu8e0p	107	12	4-TOPO	4-topo	CD
cord-265978-i0fu8e0p	107	13	plasmid	plasmid	NN
cord-265978-i0fu8e0p	107	14	to	to	TO
cord-265978-i0fu8e0p	107	15	provide	provide	VB
cord-265978-i0fu8e0p	107	16	a	a	DT
cord-265978-i0fu8e0p	107	17	constant	constant	JJ
cord-265978-i0fu8e0p	107	18	source	source	NN
cord-265978-i0fu8e0p	107	19	of	of	IN
cord-265978-i0fu8e0p	107	20	standard	standard	JJ
cord-265978-i0fu8e0p	107	21	DNA	dna	NN
cord-265978-i0fu8e0p	107	22	.	.	.
cord-265978-i0fu8e0p	108	1	The	the	DT
cord-265978-i0fu8e0p	108	2	plasmid	plasmid	NN
cord-265978-i0fu8e0p	108	3	DNA	dna	NN
cord-265978-i0fu8e0p	108	4	was	be	VBD
cord-265978-i0fu8e0p	108	5	accurately	accurately	RB
cord-265978-i0fu8e0p	108	6	quantified	quantify	VBN
cord-265978-i0fu8e0p	108	7	by	by	IN
cord-265978-i0fu8e0p	108	8	spectrophotometry	spectrophotometry	NN
cord-265978-i0fu8e0p	108	9	and	and	CC
cord-265978-i0fu8e0p	108	10	a	a	DT
cord-265978-i0fu8e0p	108	11	series	series	NN
cord-265978-i0fu8e0p	108	12	of	of	IN
cord-265978-i0fu8e0p	108	13	10-fold	10-fold	JJ
cord-265978-i0fu8e0p	108	14	dilutions	dilution	NNS
cord-265978-i0fu8e0p	108	15	starting	start	VBG
cord-265978-i0fu8e0p	108	16	from	from	IN
cord-265978-i0fu8e0p	108	17	a	a	DT
cord-265978-i0fu8e0p	108	18	DNA	dna	NN
cord-265978-i0fu8e0p	108	19	concentration	concentration	NN
cord-265978-i0fu8e0p	108	20	of	of	IN
cord-265978-i0fu8e0p	108	21	25	25	CD
cord-265978-i0fu8e0p	108	22	mg	mg	NNP
cord-265978-i0fu8e0p	108	23	/	/	SYM
cord-265978-i0fu8e0p	108	24	ml	ml	NN
cord-265978-i0fu8e0p	108	25	(	(	-LRB-
cord-265978-i0fu8e0p	108	26	equivalent	equivalent	JJ
cord-265978-i0fu8e0p	108	27	to	to	IN
cord-265978-i0fu8e0p	108	28	10	10	CD
cord-265978-i0fu8e0p	108	29	8	8	CD
cord-265978-i0fu8e0p	108	30	copies	copy	NNS
cord-265978-i0fu8e0p	108	31	per	per	IN
cord-265978-i0fu8e0p	108	32	reaction	reaction	NN
cord-265978-i0fu8e0p	108	33	)	)	-RRB-
cord-265978-i0fu8e0p	108	34	till	till	IN
cord-265978-i0fu8e0p	108	35	a	a	DT
cord-265978-i0fu8e0p	108	36	DNA	dna	NN
cord-265978-i0fu8e0p	108	37	concentration	concentration	NN
cord-265978-i0fu8e0p	108	38	of	of	IN
cord-265978-i0fu8e0p	108	39	25	25	CD
cord-265978-i0fu8e0p	108	40	fg	fg	NNP
cord-265978-i0fu8e0p	108	41	/	/	SYM
cord-265978-i0fu8e0p	108	42	ml	ml	NN
cord-265978-i0fu8e0p	108	43	(	(	-LRB-
cord-265978-i0fu8e0p	108	44	equivalent	equivalent	JJ
cord-265978-i0fu8e0p	108	45	to	to	IN
cord-265978-i0fu8e0p	108	46	10	10	CD
cord-265978-i0fu8e0p	108	47	0	0	CD
cord-265978-i0fu8e0p	108	48	copies	copy	NNS
cord-265978-i0fu8e0p	108	49	per	per	IN
cord-265978-i0fu8e0p	108	50	reaction	reaction	NN
cord-265978-i0fu8e0p	108	51	)	)	-RRB-
cord-265978-i0fu8e0p	108	52	were	be	VBD
cord-265978-i0fu8e0p	108	53	prepared	prepare	VBN
cord-265978-i0fu8e0p	108	54	.	.	.
cord-265978-i0fu8e0p	109	1	The	the	DT
cord-265978-i0fu8e0p	109	2	correlation	correlation	NN
cord-265978-i0fu8e0p	109	3	between	between	IN
cord-265978-i0fu8e0p	109	4	the	the	DT
cord-265978-i0fu8e0p	109	5	plasmid	plasmid	NN
cord-265978-i0fu8e0p	109	6	dilution	dilution	NN
cord-265978-i0fu8e0p	109	7	and	and	CC
cord-265978-i0fu8e0p	109	8	the	the	DT
cord-265978-i0fu8e0p	109	9	threshold	threshold	NN
cord-265978-i0fu8e0p	109	10	cycle	cycle	NN
cord-265978-i0fu8e0p	109	11	(	(	-LRB-
cord-265978-i0fu8e0p	109	12	C	C	NNP
cord-265978-i0fu8e0p	109	13	T	T	NNP
cord-265978-i0fu8e0p	109	14	)	)	-RRB-
cord-265978-i0fu8e0p	109	15	values	value	NNS
cord-265978-i0fu8e0p	109	16	in	in	IN
cord-265978-i0fu8e0p	109	17	qPCR	qPCR	NNP
cord-265978-i0fu8e0p	109	18	was	be	VBD
cord-265978-i0fu8e0p	109	19	analyzed	analyze	VBN
cord-265978-i0fu8e0p	109	20	by	by	IN
cord-265978-i0fu8e0p	109	21	plotting	plot	VBG
cord-265978-i0fu8e0p	109	22	a	a	DT
cord-265978-i0fu8e0p	109	23	standard	standard	JJ
cord-265978-i0fu8e0p	109	24	curve	curve	NN
cord-265978-i0fu8e0p	109	25	(	(	-LRB-
cord-265978-i0fu8e0p	109	26	Fig	Fig	NNP
cord-265978-i0fu8e0p	109	27	.	.	.
cord-265978-i0fu8e0p	109	28	1C	1C	NNP
cord-265978-i0fu8e0p	109	29	)	)	-RRB-
cord-265978-i0fu8e0p	109	30	.	.	.
cord-265978-i0fu8e0p	110	1	A	a	DT
cord-265978-i0fu8e0p	110	2	linear	linear	JJ
cord-265978-i0fu8e0p	110	3	regression	regression	NN
cord-265978-i0fu8e0p	110	4	relationship	relationship	NN
cord-265978-i0fu8e0p	110	5	was	be	VBD
cord-265978-i0fu8e0p	110	6	observed	observe	VBN
cord-265978-i0fu8e0p	110	7	with	with	IN
cord-265978-i0fu8e0p	110	8	a	a	DT
cord-265978-i0fu8e0p	110	9	coefficient	coefficient	NN
cord-265978-i0fu8e0p	110	10	of	of	IN
cord-265978-i0fu8e0p	110	11	determination	determination	NN
cord-265978-i0fu8e0p	110	12	(	(	-LRB-
cord-265978-i0fu8e0p	110	13	R	R	NNP
cord-265978-i0fu8e0p	110	14	2	2	CD
cord-265978-i0fu8e0p	110	15	)	)	-RRB-
cord-265978-i0fu8e0p	110	16	of	of	IN
cord-265978-i0fu8e0p	110	17	0.994	0.994	CD
cord-265978-i0fu8e0p	110	18	and	and	CC
cord-265978-i0fu8e0p	110	19	a	a	DT
cord-265978-i0fu8e0p	110	20	slope	slope	NN
cord-265978-i0fu8e0p	110	21	of	of	IN
cord-265978-i0fu8e0p	110	22	À3.455	à3.455	DT
cord-265978-i0fu8e0p	110	23	.	.	.
cord-265978-i0fu8e0p	111	1	These	these	DT
cord-265978-i0fu8e0p	111	2	values	value	NNS
cord-265978-i0fu8e0p	111	3	suggest	suggest	VBP
cord-265978-i0fu8e0p	111	4	an	an	DT
cord-265978-i0fu8e0p	111	5	optimum	optimum	JJ
cord-265978-i0fu8e0p	111	6	PCR	pcr	NN
cord-265978-i0fu8e0p	111	7	efficiency	efficiency	NN
cord-265978-i0fu8e0p	111	8	and	and	CC
cord-265978-i0fu8e0p	111	9	a	a	DT
cord-265978-i0fu8e0p	111	10	good	good	JJ
cord-265978-i0fu8e0p	111	11	correlation	correlation	NN
cord-265978-i0fu8e0p	111	12	between	between	IN
cord-265978-i0fu8e0p	111	13	C	C	NNP
cord-265978-i0fu8e0p	111	14	T	T	NNP
cord-265978-i0fu8e0p	111	15	values	value	NNS
cord-265978-i0fu8e0p	111	16	and	and	CC
cord-265978-i0fu8e0p	111	17	template	template	NN
cord-265978-i0fu8e0p	111	18	concentrations	concentration	NNS
cord-265978-i0fu8e0p	111	19	.	.	.
cord-265978-i0fu8e0p	112	1	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	112	2	Green	Green	NNP
cord-265978-i0fu8e0p	112	3	I	-PRON-	PRP
cord-265978-i0fu8e0p	112	4	based	base	VBD
cord-265978-i0fu8e0p	113	1	real	real	JJ
cord-265978-i0fu8e0p	113	2	-	-	HYPH
cord-265978-i0fu8e0p	113	3	time	time	NN
cord-265978-i0fu8e0p	113	4	PCR	PCR	NNP
cord-265978-i0fu8e0p	113	5	is	be	VBZ
cord-265978-i0fu8e0p	113	6	a	a	DT
cord-265978-i0fu8e0p	113	7	sequence	sequence	NN
cord-265978-i0fu8e0p	113	8	independent	independent	JJ
cord-265978-i0fu8e0p	113	9	assay	assay	NN
cord-265978-i0fu8e0p	113	10	that	that	WDT
cord-265978-i0fu8e0p	113	11	requires	require	VBZ
cord-265978-i0fu8e0p	113	12	conduction	conduction	NN
cord-265978-i0fu8e0p	113	13	of	of	IN
cord-265978-i0fu8e0p	113	14	a	a	DT
cord-265978-i0fu8e0p	113	15	melt	melt	NN
cord-265978-i0fu8e0p	113	16	curve	curve	NN
cord-265978-i0fu8e0p	113	17	analysis	analysis	NN
cord-265978-i0fu8e0p	113	18	on	on	IN
cord-265978-i0fu8e0p	113	19	the	the	DT
cord-265978-i0fu8e0p	113	20	PCR	PCR	NNP
cord-265978-i0fu8e0p	113	21	products	product	NNS
cord-265978-i0fu8e0p	113	22	.	.	.
cord-265978-i0fu8e0p	114	1	Specific	specific	JJ
cord-265978-i0fu8e0p	114	2	amplification	amplification	NN
cord-265978-i0fu8e0p	114	3	of	of	IN
cord-265978-i0fu8e0p	114	4	the	the	DT
cord-265978-i0fu8e0p	114	5	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	114	6	target	target	NN
cord-265978-i0fu8e0p	114	7	sequence	sequence	NN
cord-265978-i0fu8e0p	114	8	was	be	VBD
cord-265978-i0fu8e0p	114	9	identified	identify	VBN
cord-265978-i0fu8e0p	114	10	by	by	IN
cord-265978-i0fu8e0p	114	11	the	the	DT
cord-265978-i0fu8e0p	114	12	generation	generation	NN
cord-265978-i0fu8e0p	114	13	of	of	IN
cord-265978-i0fu8e0p	114	14	a	a	DT
cord-265978-i0fu8e0p	114	15	melt	melt	NN
cord-265978-i0fu8e0p	114	16	peak	peak	NN
cord-265978-i0fu8e0p	114	17	at	at	IN
cord-265978-i0fu8e0p	114	18	78.35	78.35	CD
cord-265978-i0fu8e0p	114	19	C	c	NN
cord-265978-i0fu8e0p	114	20	AE	AE	NNP
cord-265978-i0fu8e0p	114	21	0.26	0.26	CD
cord-265978-i0fu8e0p	114	22	.	.	.
cord-265978-i0fu8e0p	115	1	Specificity	specificity	NN
cord-265978-i0fu8e0p	115	2	of	of	IN
cord-265978-i0fu8e0p	115	3	the	the	DT
cord-265978-i0fu8e0p	115	4	reaction	reaction	NN
cord-265978-i0fu8e0p	115	5	was	be	VBD
cord-265978-i0fu8e0p	115	6	further	further	RB
cord-265978-i0fu8e0p	115	7	evaluated	evaluate	VBN
cord-265978-i0fu8e0p	115	8	for	for	IN
cord-265978-i0fu8e0p	115	9	cross	cross	NN
cord-265978-i0fu8e0p	115	10	reactivity	reactivity	NN
cord-265978-i0fu8e0p	115	11	against	against	IN
cord-265978-i0fu8e0p	115	12	different	different	JJ
cord-265978-i0fu8e0p	115	13	viruses	virus	NNS
cord-265978-i0fu8e0p	115	14	that	that	WDT
cord-265978-i0fu8e0p	115	15	show	show	VBP
cord-265978-i0fu8e0p	115	16	symptomatic	symptomatic	JJ
cord-265978-i0fu8e0p	115	17	or	or	CC
cord-265978-i0fu8e0p	115	18	genetic	genetic	JJ
cord-265978-i0fu8e0p	115	19	relationship	relationship	NN
cord-265978-i0fu8e0p	115	20	with	with	IN
cord-265978-i0fu8e0p	115	21	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	115	22	(	(	-LRB-
cord-265978-i0fu8e0p	115	23	Table	Table	NNP
cord-265978-i0fu8e0p	115	24	1	1	CD
cord-265978-i0fu8e0p	115	25	)	)	-RRB-
cord-265978-i0fu8e0p	115	26	.	.	.
cord-265978-i0fu8e0p	116	1	None	none	NN
cord-265978-i0fu8e0p	116	2	of	of	IN
cord-265978-i0fu8e0p	116	3	these	these	DT
cord-265978-i0fu8e0p	116	4	viruses	virus	NNS
cord-265978-i0fu8e0p	116	5	or	or	CC
cord-265978-i0fu8e0p	116	6	the	the	DT
cord-265978-i0fu8e0p	116	7	negative	negative	JJ
cord-265978-i0fu8e0p	116	8	controls	control	NNS
cord-265978-i0fu8e0p	116	9	showed	show	VBD
cord-265978-i0fu8e0p	116	10	specific	specific	JJ
cord-265978-i0fu8e0p	116	11	positive	positive	JJ
cord-265978-i0fu8e0p	116	12	signals	signal	NNS
cord-265978-i0fu8e0p	116	13	rather	rather	RB
cord-265978-i0fu8e0p	116	14	than	than	IN
cord-265978-i0fu8e0p	116	15	the	the	DT
cord-265978-i0fu8e0p	116	16	internal	internal	JJ
cord-265978-i0fu8e0p	116	17	control	control	NN
cord-265978-i0fu8e0p	116	18	signal	signal	NN
cord-265978-i0fu8e0p	116	19	consistent	consistent	JJ
cord-265978-i0fu8e0p	116	20	with	with	IN
cord-265978-i0fu8e0p	116	21	true	true	JJ
cord-265978-i0fu8e0p	116	22	-	-	HYPH
cord-265978-i0fu8e0p	116	23	negative	negative	JJ
cord-265978-i0fu8e0p	116	24	results	result	NNS
cord-265978-i0fu8e0p	116	25	.	.	.
cord-265978-i0fu8e0p	117	1	The	the	DT
cord-265978-i0fu8e0p	117	2	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	117	3	of	of	IN
cord-265978-i0fu8e0p	117	4	the	the	DT
cord-265978-i0fu8e0p	117	5	assay	assay	NN
cord-265978-i0fu8e0p	117	6	was	be	VBD
cord-265978-i0fu8e0p	117	7	evaluated	evaluate	VBN
cord-265978-i0fu8e0p	117	8	by	by	IN
cord-265978-i0fu8e0p	117	9	testing	test	VBG
cord-265978-i0fu8e0p	117	10	10-fold	10-fold	JJ
cord-265978-i0fu8e0p	117	11	serial	serial	JJ
cord-265978-i0fu8e0p	117	12	dilutions	dilution	NNS
cord-265978-i0fu8e0p	117	13	of	of	IN
cord-265978-i0fu8e0p	117	14	DNA	dna	NN
cord-265978-i0fu8e0p	117	15	standards	standard	NNS
cord-265978-i0fu8e0p	117	16	(	(	-LRB-
cord-265978-i0fu8e0p	117	17	10	10	CD
cord-265978-i0fu8e0p	117	18	8	8	CD
cord-265978-i0fu8e0p	117	19	to	to	TO
cord-265978-i0fu8e0p	117	20	10	10	CD
cord-265978-i0fu8e0p	117	21	0	0	CD
cord-265978-i0fu8e0p	117	22	copies	copy	NNS
cord-265978-i0fu8e0p	117	23	)	)	-RRB-
cord-265978-i0fu8e0p	117	24	.	.	.
cord-265978-i0fu8e0p	118	1	The	the	DT
cord-265978-i0fu8e0p	118	2	results	result	NNS
cord-265978-i0fu8e0p	118	3	showed	show	VBD
cord-265978-i0fu8e0p	118	4	that	that	IN
cord-265978-i0fu8e0p	118	5	the	the	DT
cord-265978-i0fu8e0p	118	6	assay	assay	NN
cord-265978-i0fu8e0p	118	7	could	could	MD
cord-265978-i0fu8e0p	118	8	detect	detect	VB
cord-265978-i0fu8e0p	118	9	down	down	RP
cord-265978-i0fu8e0p	118	10	to	to	TO
cord-265978-i0fu8e0p	118	11	10	10	CD
cord-265978-i0fu8e0p	118	12	3	3	CD
cord-265978-i0fu8e0p	118	13	copies	copy	NNS
cord-265978-i0fu8e0p	118	14	of	of	IN
cord-265978-i0fu8e0p	118	15	DNA	dna	NN
cord-265978-i0fu8e0p	118	16	standard	standard	NN
cord-265978-i0fu8e0p	118	17	per	per	IN
cord-265978-i0fu8e0p	118	18	reaction	reaction	NN
cord-265978-i0fu8e0p	118	19	.	.	.
cord-265978-i0fu8e0p	119	1	When	when	WRB
cord-265978-i0fu8e0p	119	2	testing	test	VBG
cord-265978-i0fu8e0p	119	3	a	a	DT
cord-265978-i0fu8e0p	119	4	dilution	dilution	NN
cord-265978-i0fu8e0p	119	5	series	series	NN
cord-265978-i0fu8e0p	119	6	of	of	IN
cord-265978-i0fu8e0p	119	7	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	119	8	reference	reference	NN
cord-265978-i0fu8e0p	119	9	strain	strain	NN
cord-265978-i0fu8e0p	119	10	in	in	IN
cord-265978-i0fu8e0p	119	11	nasal/	nasal/	CD
cord-265978-i0fu8e0p	119	12	fecal	fecal	JJ
cord-265978-i0fu8e0p	119	13	suspension	suspension	NN
cord-265978-i0fu8e0p	119	14	(	(	-LRB-
cord-265978-i0fu8e0p	119	15	10	10	CD
cord-265978-i0fu8e0p	119	16	5	5	CD
cord-265978-i0fu8e0p	119	17	TCID	TCID	NNP
cord-265978-i0fu8e0p	119	18	50	50	CD
cord-265978-i0fu8e0p	120	1	/ml	/ml	NFP
cord-265978-i0fu8e0p	120	2	,	,	,
cord-265978-i0fu8e0p	120	3	dilutions	dilution	NNS
cord-265978-i0fu8e0p	120	4	10	10	CD
cord-265978-i0fu8e0p	120	5	À1	à1	CD
cord-265978-i0fu8e0p	120	6	e10	e10	NN
cord-265978-i0fu8e0p	120	7	À6	À6	NNP
cord-265978-i0fu8e0p	120	8	)	)	-RRB-
cord-265978-i0fu8e0p	120	9	,	,	,
cord-265978-i0fu8e0p	120	10	the	the	DT
cord-265978-i0fu8e0p	120	11	obtained	obtain	VBN
cord-265978-i0fu8e0p	120	12	detection	detection	NN
cord-265978-i0fu8e0p	120	13	limit	limit	NN
cord-265978-i0fu8e0p	120	14	was	be	VBD
cord-265978-i0fu8e0p	120	15	1	1	CD
cord-265978-i0fu8e0p	120	16	TCID	TCID	NNP
cord-265978-i0fu8e0p	120	17	50	50	CD
cord-265978-i0fu8e0p	120	18	/ml	/ml	.
cord-265978-i0fu8e0p	120	19	;	;	:
cord-265978-i0fu8e0p	120	20	equivalent	equivalent	JJ
cord-265978-i0fu8e0p	120	21	to	to	IN
cord-265978-i0fu8e0p	120	22	1.17	1.17	CD
cord-265978-i0fu8e0p	120	23	Â	â	CD
cord-265978-i0fu8e0p	120	24	10	10	CD
cord-265978-i0fu8e0p	120	25	À3	à3	CD
cord-265978-i0fu8e0p	120	26	TCID	TCID	NNP
cord-265978-i0fu8e0p	120	27	50	50	CD
cord-265978-i0fu8e0p	120	28	/	/	SYM
cord-265978-i0fu8e0p	120	29	reaction	reaction	NN
cord-265978-i0fu8e0p	120	30	(	(	-LRB-
cord-265978-i0fu8e0p	120	31	Fig	Fig	NNP
cord-265978-i0fu8e0p	120	32	.	.	.
cord-265978-i0fu8e0p	120	33	1A	1a	NN
cord-265978-i0fu8e0p	120	34	,	,	,
cord-265978-i0fu8e0p	120	35	B	b	NN
cord-265978-i0fu8e0p	120	36	)	)	-RRB-
cord-265978-i0fu8e0p	120	37	.	.	.
cord-265978-i0fu8e0p	121	1	In	in	IN
cord-265978-i0fu8e0p	121	2	comparison	comparison	NN
cord-265978-i0fu8e0p	121	3	,	,	,
cord-265978-i0fu8e0p	121	4	the	the	DT
cord-265978-i0fu8e0p	121	5	conventional	conventional	JJ
cord-265978-i0fu8e0p	121	6	RT	RT	NNP
cord-265978-i0fu8e0p	121	7	-	-	HYPH
cord-265978-i0fu8e0p	121	8	PCR	PCR	NNP
cord-265978-i0fu8e0p	121	9	was	be	VBD
cord-265978-i0fu8e0p	121	10	10	10	CD
cord-265978-i0fu8e0p	121	11	times	time	NNS
cord-265978-i0fu8e0p	121	12	less	less	RBR
cord-265978-i0fu8e0p	121	13	sensitive	sensitive	JJ
cord-265978-i0fu8e0p	121	14	than	than	IN
cord-265978-i0fu8e0p	121	15	the	the	DT
cord-265978-i0fu8e0p	121	16	real	real	JJ
cord-265978-i0fu8e0p	121	17	-	-	HYPH
cord-265978-i0fu8e0p	121	18	time	time	NN
cord-265978-i0fu8e0p	121	19	assay	assay	NN
cord-265978-i0fu8e0p	121	20	for	for	IN
cord-265978-i0fu8e0p	121	21	both	both	DT
cord-265978-i0fu8e0p	121	22	template	template	NN
cord-265978-i0fu8e0p	121	23	systems	system	NNS
cord-265978-i0fu8e0p	121	24	.	.	.
cord-265978-i0fu8e0p	122	1	Accuracy	accuracy	NN
cord-265978-i0fu8e0p	122	2	and	and	CC
cord-265978-i0fu8e0p	122	3	reproducibility	reproducibility	NN
cord-265978-i0fu8e0p	122	4	of	of	IN
cord-265978-i0fu8e0p	122	5	the	the	DT
cord-265978-i0fu8e0p	122	6	assay	assay	NN
cord-265978-i0fu8e0p	122	7	were	be	VBD
cord-265978-i0fu8e0p	122	8	established	establish	VBN
cord-265978-i0fu8e0p	122	9	basing	base	VBG
cord-265978-i0fu8e0p	122	10	on	on	IN
cord-265978-i0fu8e0p	122	11	the	the	DT
cord-265978-i0fu8e0p	122	12	C	c	NN
cord-265978-i0fu8e0p	122	13	T	T	NNP
cord-265978-i0fu8e0p	122	14	values	value	NNS
cord-265978-i0fu8e0p	122	15	obtained	obtain	VBN
cord-265978-i0fu8e0p	122	16	from	from	IN
cord-265978-i0fu8e0p	122	17	testing	test	VBG
cord-265978-i0fu8e0p	122	18	DNA	dna	NN
cord-265978-i0fu8e0p	122	19	standards	standard	NNS
cord-265978-i0fu8e0p	122	20	in	in	IN
cord-265978-i0fu8e0p	122	21	triplicates	triplicate	NNS
cord-265978-i0fu8e0p	122	22	;	;	:
cord-265978-i0fu8e0p	122	23	within	within	IN
cord-265978-i0fu8e0p	122	24	each	each	DT
cord-265978-i0fu8e0p	122	25	run	run	NN
cord-265978-i0fu8e0p	122	26	(	(	-LRB-
cord-265978-i0fu8e0p	122	27	intra	intra	JJ
cord-265978-i0fu8e0p	122	28	-	-	JJ
cord-265978-i0fu8e0p	122	29	assay	assay	JJ
cord-265978-i0fu8e0p	122	30	)	)	-RRB-
cord-265978-i0fu8e0p	122	31	and	and	CC
cord-265978-i0fu8e0p	122	32	in	in	IN
cord-265978-i0fu8e0p	122	33	three	three	CD
cord-265978-i0fu8e0p	122	34	consecutive	consecutive	JJ
cord-265978-i0fu8e0p	122	35	runs	run	NNS
cord-265978-i0fu8e0p	122	36	(	(	-LRB-
cord-265978-i0fu8e0p	122	37	inter	inter	JJ
cord-265978-i0fu8e0p	122	38	-	-	JJ
cord-265978-i0fu8e0p	122	39	assay	assay	NN
cord-265978-i0fu8e0p	122	40	)	)	-RRB-
cord-265978-i0fu8e0p	122	41	(	(	-LRB-
cord-265978-i0fu8e0p	122	42	Table	table	NN
cord-265978-i0fu8e0p	122	43	3	3	CD
cord-265978-i0fu8e0p	122	44	)	)	-RRB-
cord-265978-i0fu8e0p	122	45	.	.	.
cord-265978-i0fu8e0p	123	1	The	the	DT
cord-265978-i0fu8e0p	123	2	calculated	calculated	JJ
cord-265978-i0fu8e0p	123	3	S.D	S.D	NNP
cord-265978-i0fu8e0p	123	4	and	and	CC
cord-265978-i0fu8e0p	123	5	C.V	C.V	NNP
cord-265978-i0fu8e0p	123	6	values	value	NNS
cord-265978-i0fu8e0p	123	7	for	for	IN
cord-265978-i0fu8e0p	123	8	intra	intra	JJ
cord-265978-i0fu8e0p	123	9	-	-	JJ
cord-265978-i0fu8e0p	123	10	assay	assay	JJ
cord-265978-i0fu8e0p	123	11	variability	variability	NN
cord-265978-i0fu8e0p	123	12	were	be	VBD
cord-265978-i0fu8e0p	123	13	low	low	JJ
cord-265978-i0fu8e0p	123	14	,	,	,
cord-265978-i0fu8e0p	123	15	ranging	range	VBG
cord-265978-i0fu8e0p	123	16	from	from	IN
cord-265978-i0fu8e0p	123	17	0.03	0.03	CD
cord-265978-i0fu8e0p	123	18	to	to	TO
cord-265978-i0fu8e0p	123	19	0.53	0.53	CD
cord-265978-i0fu8e0p	123	20	and	and	CC
cord-265978-i0fu8e0p	123	21	from	from	IN
cord-265978-i0fu8e0p	123	22	0.13	0.13	CD
cord-265978-i0fu8e0p	123	23	%	%	NN
cord-265978-i0fu8e0p	123	24	to	to	TO
cord-265978-i0fu8e0p	123	25	1.25	1.25	CD
cord-265978-i0fu8e0p	123	26	%	%	NN
cord-265978-i0fu8e0p	123	27	,	,	,
cord-265978-i0fu8e0p	123	28	respectively	respectively	RB
cord-265978-i0fu8e0p	123	29	.	.	.
cord-265978-i0fu8e0p	124	1	The	the	DT
cord-265978-i0fu8e0p	124	2	inter	inter	JJ
cord-265978-i0fu8e0p	124	3	-	-	JJ
cord-265978-i0fu8e0p	124	4	assay	assay	JJ
cord-265978-i0fu8e0p	124	5	variability	variability	NN
cord-265978-i0fu8e0p	124	6	was	be	VBD
cord-265978-i0fu8e0p	124	7	slightly	slightly	RB
cord-265978-i0fu8e0p	124	8	higher	high	JJR
cord-265978-i0fu8e0p	124	9	with	with	IN
cord-265978-i0fu8e0p	124	10	S.D	S.D	NNP
cord-265978-i0fu8e0p	124	11	ranged	range	VBD
cord-265978-i0fu8e0p	124	12	from	from	IN
cord-265978-i0fu8e0p	124	13	0.07	0.07	CD
cord-265978-i0fu8e0p	124	14	to	to	IN
cord-265978-i0fu8e0p	124	15	0.51	0.51	CD
cord-265978-i0fu8e0p	124	16	and	and	CC
cord-265978-i0fu8e0p	124	17	C.V	C.V	NNP
cord-265978-i0fu8e0p	124	18	ranged	range	VBD
cord-265978-i0fu8e0p	124	19	from	from	IN
cord-265978-i0fu8e0p	124	20	0.22	0.22	CD
cord-265978-i0fu8e0p	124	21	%	%	NN
cord-265978-i0fu8e0p	124	22	to	to	IN
cord-265978-i0fu8e0p	124	23	2.5	2.5	CD
cord-265978-i0fu8e0p	124	24	%	%	NN
cord-265978-i0fu8e0p	124	25	.	.	.
cord-265978-i0fu8e0p	125	1	The	the	DT
cord-265978-i0fu8e0p	125	2	dynamic	dynamic	JJ
cord-265978-i0fu8e0p	125	3	range	range	NN
cord-265978-i0fu8e0p	125	4	of	of	IN
cord-265978-i0fu8e0p	125	5	the	the	DT
cord-265978-i0fu8e0p	125	6	developed	develop	VBN
cord-265978-i0fu8e0p	125	7	qPCR	qpcr	NN
cord-265978-i0fu8e0p	125	8	assay	assay	NNP
cord-265978-i0fu8e0p	125	9	was	be	VBD
cord-265978-i0fu8e0p	125	10	evaluated	evaluate	VBN
cord-265978-i0fu8e0p	125	11	by	by	IN
cord-265978-i0fu8e0p	125	12	carrying	carry	VBG
cord-265978-i0fu8e0p	125	13	out	out	RP
cord-265978-i0fu8e0p	125	14	10-fold	10-fold	CD
cord-265978-i0fu8e0p	125	15	dilution	dilution	NN
cord-265978-i0fu8e0p	125	16	series	series	NN
cord-265978-i0fu8e0p	125	17	of	of	IN
cord-265978-i0fu8e0p	125	18	:	:	:
cord-265978-i0fu8e0p	125	19	a	a	LS
cord-265978-i0fu8e0p	125	20	)	)	-RRB-
cord-265978-i0fu8e0p	125	21	DNA	dna	NN
cord-265978-i0fu8e0p	125	22	plasmid	plasmid	NN
cord-265978-i0fu8e0p	125	23	standard	standard	NN
cord-265978-i0fu8e0p	125	24	in	in	IN
cord-265978-i0fu8e0p	125	25	water	water	NN
cord-265978-i0fu8e0p	125	26	;	;	:
cord-265978-i0fu8e0p	125	27	b	b	LS
cord-265978-i0fu8e0p	125	28	)	)	-RRB-
cord-265978-i0fu8e0p	125	29	BCoV	bcov	NN
cord-265978-i0fu8e0p	125	30	reference	reference	NN
cord-265978-i0fu8e0p	125	31	strain	strain	NN
cord-265978-i0fu8e0p	125	32	spiked	spike	VBN
cord-265978-i0fu8e0p	125	33	in	in	IN
cord-265978-i0fu8e0p	125	34	nasal	nasal	NN
cord-265978-i0fu8e0p	125	35	/	/	SYM
cord-265978-i0fu8e0p	125	36	fecal	fecal	JJ
cord-265978-i0fu8e0p	125	37	suspension	suspension	NN
cord-265978-i0fu8e0p	125	38	of	of	IN
cord-265978-i0fu8e0p	125	39	non	non	JJ
cord-265978-i0fu8e0p	125	40	-	-	JJ
cord-265978-i0fu8e0p	125	41	infected	infected	JJ
cord-265978-i0fu8e0p	125	42	calves	calf	NNS
cord-265978-i0fu8e0p	125	43	;	;	:
cord-265978-i0fu8e0p	125	44	and	and	CC
cord-265978-i0fu8e0p	125	45	c	c	LS
cord-265978-i0fu8e0p	125	46	)	)	-RRB-
cord-265978-i0fu8e0p	125	47	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	125	48	cDNA	cdna	NN
cord-265978-i0fu8e0p	125	49	in	in	IN
cord-265978-i0fu8e0p	125	50	water	water	NN
cord-265978-i0fu8e0p	125	51	.	.	.
cord-265978-i0fu8e0p	126	1	Against	against	IN
cord-265978-i0fu8e0p	126	2	the	the	DT
cord-265978-i0fu8e0p	126	3	DNA	dna	NN
cord-265978-i0fu8e0p	126	4	standard	standard	NN
cord-265978-i0fu8e0p	126	5	and	and	CC
cord-265978-i0fu8e0p	126	6	the	the	DT
cord-265978-i0fu8e0p	126	7	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	126	8	spiked	spike	VBD
cord-265978-i0fu8e0p	126	9	in	in	IN
cord-265978-i0fu8e0p	126	10	nasal	nasal	NN
cord-265978-i0fu8e0p	126	11	/	/	SYM
cord-265978-i0fu8e0p	126	12	fecal	fecal	JJ
cord-265978-i0fu8e0p	126	13	suspension	suspension	NN
cord-265978-i0fu8e0p	126	14	,	,	,
cord-265978-i0fu8e0p	126	15	the	the	DT
cord-265978-i0fu8e0p	126	16	PCR	PCR	NNP
cord-265978-i0fu8e0p	126	17	was	be	VBD
cord-265978-i0fu8e0p	126	18	able	able	JJ
cord-265978-i0fu8e0p	126	19	to	to	TO
cord-265978-i0fu8e0p	126	20	detect	detect	VB
cord-265978-i0fu8e0p	126	21	6	6	CD
cord-265978-i0fu8e0p	126	22	fold	fold	VB
cord-265978-i0fu8e0p	126	23	differences	difference	NNS
cord-265978-i0fu8e0p	126	24	in	in	IN
cord-265978-i0fu8e0p	126	25	the	the	DT
cord-265978-i0fu8e0p	126	26	starting	starting	NN
cord-265978-i0fu8e0p	126	27	template	template	NN
cord-265978-i0fu8e0p	126	28	concentration	concentration	NN
cord-265978-i0fu8e0p	126	29	over	over	IN
cord-265978-i0fu8e0p	126	30	a	a	DT
cord-265978-i0fu8e0p	126	31	range	range	NN
cord-265978-i0fu8e0p	126	32	of	of	IN
cord-265978-i0fu8e0p	126	33	10	10	CD
cord-265978-i0fu8e0p	126	34	3	3	CD
cord-265978-i0fu8e0p	126	35	e10	e10	NN
cord-265978-i0fu8e0p	126	36	8	8	CD
cord-265978-i0fu8e0p	126	37	copies	copy	NNS
cord-265978-i0fu8e0p	126	38	/	/	SYM
cord-265978-i0fu8e0p	126	39	reaction	reaction	NN
cord-265978-i0fu8e0p	126	40	and	and	CC
cord-265978-i0fu8e0p	126	41	10	10	CD
cord-265978-i0fu8e0p	126	42	5	5	CD
cord-265978-i0fu8e0p	126	43	e10	e10	NN
cord-265978-i0fu8e0p	126	44	0	0	CD
cord-265978-i0fu8e0p	126	45	TCID	TCID	NNP
cord-265978-i0fu8e0p	126	46	50	50	CD
cord-265978-i0fu8e0p	126	47	/ml	/ml	NFP
cord-265978-i0fu8e0p	126	48	,	,	,
cord-265978-i0fu8e0p	126	49	respectively	respectively	RB
cord-265978-i0fu8e0p	126	50	(	(	-LRB-
cord-265978-i0fu8e0p	126	51	Fig	Fig	NNP
cord-265978-i0fu8e0p	126	52	.	.	.
cord-265978-i0fu8e0p	126	53	1	1	CD
cord-265978-i0fu8e0p	126	54	)	)	-RRB-
cord-265978-i0fu8e0p	126	55	.	.	.
cord-265978-i0fu8e0p	127	1	However	however	RB
cord-265978-i0fu8e0p	127	2	,	,	,
cord-265978-i0fu8e0p	127	3	the	the	DT
cord-265978-i0fu8e0p	127	4	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	127	5	cDNA	cdna	NN
cord-265978-i0fu8e0p	127	6	spiked	spike	VBN
cord-265978-i0fu8e0p	127	7	in	in	IN
cord-265978-i0fu8e0p	127	8	water	water	NN
cord-265978-i0fu8e0p	127	9	was	be	VBD
cord-265978-i0fu8e0p	127	10	detected	detect	VBN
cord-265978-i0fu8e0p	127	11	more	more	RBR
cord-265978-i0fu8e0p	127	12	efficiently	efficiently	RB
cord-265978-i0fu8e0p	127	13	to	to	IN
cord-265978-i0fu8e0p	127	14	the	the	DT
cord-265978-i0fu8e0p	127	15	extent	extent	NN
cord-265978-i0fu8e0p	127	16	of	of	IN
cord-265978-i0fu8e0p	127	17	8	8	CD
cord-265978-i0fu8e0p	127	18	logs	log	NNS
cord-265978-i0fu8e0p	127	19	of	of	IN
cord-265978-i0fu8e0p	127	20	dilution	dilution	NN
cord-265978-i0fu8e0p	127	21	(	(	-LRB-
cord-265978-i0fu8e0p	127	22	Data	data	NN
cord-265978-i0fu8e0p	127	23	not	not	RB
cord-265978-i0fu8e0p	127	24	shown	show	VBN
cord-265978-i0fu8e0p	127	25	)	)	-RRB-
cord-265978-i0fu8e0p	127	26	.	.	.
cord-265978-i0fu8e0p	128	1	The	the	DT
cord-265978-i0fu8e0p	128	2	competence	competence	NN
cord-265978-i0fu8e0p	128	3	of	of	IN
cord-265978-i0fu8e0p	128	4	the	the	DT
cord-265978-i0fu8e0p	128	5	developed	develop	VBN
cord-265978-i0fu8e0p	128	6	real	real	JJ
cord-265978-i0fu8e0p	128	7	-	-	HYPH
cord-265978-i0fu8e0p	128	8	time	time	NN
cord-265978-i0fu8e0p	128	9	RT	RT	NNP
cord-265978-i0fu8e0p	128	10	-	-	HYPH
cord-265978-i0fu8e0p	128	11	PCR	PCR	NNP
cord-265978-i0fu8e0p	128	12	assay	assay	NN
cord-265978-i0fu8e0p	128	13	,	,	,
cord-265978-i0fu8e0p	128	14	for	for	IN
cord-265978-i0fu8e0p	128	15	accurate	accurate	JJ
cord-265978-i0fu8e0p	128	16	detection	detection	NN
cord-265978-i0fu8e0p	128	17	of	of	IN
cord-265978-i0fu8e0p	128	18	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	128	19	in	in	IN
cord-265978-i0fu8e0p	128	20	clinical	clinical	JJ
cord-265978-i0fu8e0p	128	21	samples	sample	NNS
cord-265978-i0fu8e0p	128	22	,	,	,
cord-265978-i0fu8e0p	128	23	was	be	VBD
cord-265978-i0fu8e0p	128	24	evaluated	evaluate	VBN
cord-265978-i0fu8e0p	128	25	by	by	IN
cord-265978-i0fu8e0p	128	26	analysis	analysis	NN
cord-265978-i0fu8e0p	128	27	of	of	IN
cord-265978-i0fu8e0p	128	28	103	103	CD
cord-265978-i0fu8e0p	128	29	swab	swab	NN
cord-265978-i0fu8e0p	128	30	samples	sample	NNS
cord-265978-i0fu8e0p	128	31	(	(	-LRB-
cord-265978-i0fu8e0p	128	32	68	68	CD
cord-265978-i0fu8e0p	128	33	fecal	fecal	JJ
cord-265978-i0fu8e0p	128	34	and	and	CC
cord-265978-i0fu8e0p	128	35	35	35	CD
cord-265978-i0fu8e0p	128	36	nasal	nasal	NN
cord-265978-i0fu8e0p	128	37	)	)	-RRB-
cord-265978-i0fu8e0p	128	38	in	in	IN
cord-265978-i0fu8e0p	128	39	comparison	comparison	NN
cord-265978-i0fu8e0p	128	40	to	to	IN
cord-265978-i0fu8e0p	128	41	the	the	DT
cord-265978-i0fu8e0p	128	42	gel	gel	NN
cord-265978-i0fu8e0p	128	43	-	-	HYPH
cord-265978-i0fu8e0p	128	44	based	base	VBN
cord-265978-i0fu8e0p	128	45	RT	RT	NNP
cord-265978-i0fu8e0p	128	46	-	-	HYPH
cord-265978-i0fu8e0p	128	47	PCR	PCR	NNP
cord-265978-i0fu8e0p	128	48	assay	assay	NN
cord-265978-i0fu8e0p	128	49	(	(	-LRB-
cord-265978-i0fu8e0p	128	50	Table	Table	NNP
cord-265978-i0fu8e0p	128	51	4	4	CD
cord-265978-i0fu8e0p	128	52	)	)	-RRB-
cord-265978-i0fu8e0p	128	53	.	.	.
cord-265978-i0fu8e0p	129	1	The	the	DT
cord-265978-i0fu8e0p	129	2	results	result	NNS
cord-265978-i0fu8e0p	129	3	of	of	IN
cord-265978-i0fu8e0p	129	4	both	both	DT
cord-265978-i0fu8e0p	129	5	assays	assay	NNS
cord-265978-i0fu8e0p	129	6	were	be	VBD
cord-265978-i0fu8e0p	129	7	similar	similar	JJ
cord-265978-i0fu8e0p	129	8	for	for	IN
cord-265978-i0fu8e0p	129	9	the	the	DT
cord-265978-i0fu8e0p	129	10	evaluation	evaluation	NN
cord-265978-i0fu8e0p	129	11	of	of	IN
cord-265978-i0fu8e0p	129	12	65	65	CD
cord-265978-i0fu8e0p	129	13	fecal	fecal	JJ
cord-265978-i0fu8e0p	129	14	samples	sample	NNS
cord-265978-i0fu8e0p	129	15	(	(	-LRB-
cord-265978-i0fu8e0p	129	16	17	17	CD
cord-265978-i0fu8e0p	129	17	positive	positive	JJ
cord-265978-i0fu8e0p	129	18	and	and	CC
cord-265978-i0fu8e0p	129	19	48	48	CD
cord-265978-i0fu8e0p	129	20	negative	negative	JJ
cord-265978-i0fu8e0p	129	21	)	)	-RRB-
cord-265978-i0fu8e0p	129	22	and	and	CC
cord-265978-i0fu8e0p	129	23	33	33	CD
cord-265978-i0fu8e0p	129	24	nasal	nasal	NN
cord-265978-i0fu8e0p	129	25	samples	sample	NNS
cord-265978-i0fu8e0p	129	26	(	(	-LRB-
cord-265978-i0fu8e0p	129	27	12	12	CD
cord-265978-i0fu8e0p	129	28	positive	positive	JJ
cord-265978-i0fu8e0p	129	29	and	and	CC
cord-265978-i0fu8e0p	129	30	21	21	CD
cord-265978-i0fu8e0p	129	31	negative	negative	JJ
cord-265978-i0fu8e0p	129	32	)	)	-RRB-
cord-265978-i0fu8e0p	129	33	.	.	.
cord-265978-i0fu8e0p	130	1	However	however	RB
cord-265978-i0fu8e0p	130	2	,	,	,
cord-265978-i0fu8e0p	130	3	three	three	CD
cord-265978-i0fu8e0p	130	4	fecal	fecal	JJ
cord-265978-i0fu8e0p	130	5	and	and	CC
cord-265978-i0fu8e0p	130	6	two	two	CD
cord-265978-i0fu8e0p	130	7	nasal	nasal	NN
cord-265978-i0fu8e0p	130	8	samples	sample	NNS
cord-265978-i0fu8e0p	130	9	tested	test	VBN
cord-265978-i0fu8e0p	130	10	negative	negative	JJ
cord-265978-i0fu8e0p	130	11	by	by	IN
cord-265978-i0fu8e0p	130	12	gelbased	gelbased	JJ
cord-265978-i0fu8e0p	130	13	RT	RT	NNP
cord-265978-i0fu8e0p	130	14	-	-	HYPH
cord-265978-i0fu8e0p	130	15	PCR	PCR	NNP
cord-265978-i0fu8e0p	130	16	were	be	VBD
cord-265978-i0fu8e0p	130	17	found	find	VBN
cord-265978-i0fu8e0p	130	18	to	to	TO
cord-265978-i0fu8e0p	130	19	be	be	VB
cord-265978-i0fu8e0p	130	20	positive	positive	JJ
cord-265978-i0fu8e0p	130	21	by	by	IN
cord-265978-i0fu8e0p	130	22	real	real	JJ
cord-265978-i0fu8e0p	130	23	-	-	HYPH
cord-265978-i0fu8e0p	130	24	time	time	NN
cord-265978-i0fu8e0p	130	25	RT	RT	NNP
cord-265978-i0fu8e0p	130	26	-	-	HYPH
cord-265978-i0fu8e0p	130	27	PCR	PCR	NNP
cord-265978-i0fu8e0p	130	28	.	.	.
cord-265978-i0fu8e0p	131	1	None	none	NN
cord-265978-i0fu8e0p	131	2	of	of	IN
cord-265978-i0fu8e0p	131	3	the	the	DT
cord-265978-i0fu8e0p	131	4	samples	sample	NNS
cord-265978-i0fu8e0p	131	5	tested	test	VBN
cord-265978-i0fu8e0p	131	6	negative	negative	JJ
cord-265978-i0fu8e0p	131	7	by	by	IN
cord-265978-i0fu8e0p	132	1	real	real	JJ
cord-265978-i0fu8e0p	132	2	-	-	HYPH
cord-265978-i0fu8e0p	132	3	time	time	NN
cord-265978-i0fu8e0p	132	4	RT	RT	NNP
cord-265978-i0fu8e0p	132	5	-	-	HYPH
cord-265978-i0fu8e0p	132	6	PCR	PCR	NNP
cord-265978-i0fu8e0p	132	7	was	be	VBD
cord-265978-i0fu8e0p	132	8	found	find	VBN
cord-265978-i0fu8e0p	132	9	positive	positive	JJ
cord-265978-i0fu8e0p	132	10	by	by	IN
cord-265978-i0fu8e0p	132	11	the	the	DT
cord-265978-i0fu8e0p	132	12	gel	gel	NN
cord-265978-i0fu8e0p	132	13	-	-	HYPH
cord-265978-i0fu8e0p	132	14	based	base	VBN
cord-265978-i0fu8e0p	132	15	method	method	NN
cord-265978-i0fu8e0p	132	16	.	.	.
cord-265978-i0fu8e0p	133	1	The	the	DT
cord-265978-i0fu8e0p	133	2	concentration	concentration	NN
cord-265978-i0fu8e0p	133	3	of	of	IN
cord-265978-i0fu8e0p	133	4	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	133	5	RNA	RNA	NNP
cord-265978-i0fu8e0p	133	6	in	in	IN
cord-265978-i0fu8e0p	133	7	the	the	DT
cord-265978-i0fu8e0p	133	8	analyzed	analyze	VBN
cord-265978-i0fu8e0p	133	9	positive	positive	JJ
cord-265978-i0fu8e0p	133	10	samples	sample	NNS
cord-265978-i0fu8e0p	133	11	varied	vary	VBN
cord-265978-i0fu8e0p	133	12	from	from	IN
cord-265978-i0fu8e0p	133	13	2	2	CD
cord-265978-i0fu8e0p	133	14	Â	â	CD
cord-265978-i0fu8e0p	133	15	10	10	CD
cord-265978-i0fu8e0p	133	16	4	4	CD
cord-265978-i0fu8e0p	133	17	to	to	IN
cord-265978-i0fu8e0p	133	18	1.56	1.56	CD
cord-265978-i0fu8e0p	133	19	Â	â	CD
cord-265978-i0fu8e0p	133	20	10	10	CD
cord-265978-i0fu8e0p	133	21	8	8	CD
cord-265978-i0fu8e0p	133	22	copies	copy	NNS
cord-265978-i0fu8e0p	133	23	/	/	SYM
cord-265978-i0fu8e0p	133	24	ml	ml	NN
cord-265978-i0fu8e0p	133	25	for	for	IN
cord-265978-i0fu8e0p	133	26	the	the	DT
cord-265978-i0fu8e0p	133	27	fecal	fecal	JJ
cord-265978-i0fu8e0p	133	28	samples	sample	NNS
cord-265978-i0fu8e0p	133	29	,	,	,
cord-265978-i0fu8e0p	133	30	and	and	CC
cord-265978-i0fu8e0p	133	31	3.77	3.77	CD
cord-265978-i0fu8e0p	133	32	Â	â	CD
cord-265978-i0fu8e0p	133	33	10	10	CD
cord-265978-i0fu8e0p	133	34	2	2	CD
cord-265978-i0fu8e0p	133	35	to	to	IN
cord-265978-i0fu8e0p	133	36	1.56	1.56	CD
cord-265978-i0fu8e0p	133	37	Â	â	CD
cord-265978-i0fu8e0p	133	38	10	10	CD
cord-265978-i0fu8e0p	133	39	8	8	CD
cord-265978-i0fu8e0p	133	40	copies/	copies/	CD
cord-265978-i0fu8e0p	133	41	ml	ml	NN
cord-265978-i0fu8e0p	133	42	for	for	IN
cord-265978-i0fu8e0p	133	43	the	the	DT
cord-265978-i0fu8e0p	133	44	nasal	nasal	NN
cord-265978-i0fu8e0p	133	45	samples	sample	NNS
cord-265978-i0fu8e0p	133	46	.	.	.
cord-265978-i0fu8e0p	134	1	It	-PRON-	PRP
cord-265978-i0fu8e0p	134	2	is	be	VBZ
cord-265978-i0fu8e0p	134	3	worthwhile	worthwhile	JJ
cord-265978-i0fu8e0p	134	4	to	to	TO
cord-265978-i0fu8e0p	134	5	mention	mention	VB
cord-265978-i0fu8e0p	134	6	that	that	IN
cord-265978-i0fu8e0p	134	7	the	the	DT
cord-265978-i0fu8e0p	134	8	samples	sample	NNS
cord-265978-i0fu8e0p	134	9	tested	test	VBD
cord-265978-i0fu8e0p	134	10	positive	positive	JJ
cord-265978-i0fu8e0p	134	11	in	in	IN
cord-265978-i0fu8e0p	134	12	real	real	JJ
cord-265978-i0fu8e0p	134	13	-	-	HYPH
cord-265978-i0fu8e0p	134	14	time	time	NN
cord-265978-i0fu8e0p	134	15	RT	RT	NNP
cord-265978-i0fu8e0p	134	16	-	-	HYPH
cord-265978-i0fu8e0p	134	17	PCR	PCR	NNP
cord-265978-i0fu8e0p	134	18	and	and	CC
cord-265978-i0fu8e0p	134	19	negative	negative	JJ
cord-265978-i0fu8e0p	134	20	in	in	IN
cord-265978-i0fu8e0p	134	21	gelbased	gelbased	JJ
cord-265978-i0fu8e0p	134	22	assay	assay	NN
cord-265978-i0fu8e0p	134	23	contained	contain	VBD
cord-265978-i0fu8e0p	134	24	the	the	DT
cord-265978-i0fu8e0p	134	25	least	least	JJS
cord-265978-i0fu8e0p	134	26	virus	virus	NN
cord-265978-i0fu8e0p	134	27	RNA	RNA	NNP
cord-265978-i0fu8e0p	134	28	concentration	concentration	NN
cord-265978-i0fu8e0p	134	29	that	that	WDT
cord-265978-i0fu8e0p	134	30	ranged	range	VBD
cord-265978-i0fu8e0p	134	31	from	from	IN
cord-265978-i0fu8e0p	134	32	2	2	CD
cord-265978-i0fu8e0p	134	33	Â	â	CD
cord-265978-i0fu8e0p	134	34	10	10	CD
cord-265978-i0fu8e0p	134	35	4	4	CD
cord-265978-i0fu8e0p	134	36	to	to	IN
cord-265978-i0fu8e0p	134	37	3.47	3.47	CD
cord-265978-i0fu8e0p	134	38	Â	â	CD
cord-265978-i0fu8e0p	134	39	10	10	CD
cord-265978-i0fu8e0p	134	40	4	4	CD
cord-265978-i0fu8e0p	134	41	for	for	IN
cord-265978-i0fu8e0p	134	42	the	the	DT
cord-265978-i0fu8e0p	134	43	fecal	fecal	JJ
cord-265978-i0fu8e0p	134	44	samples	sample	NNS
cord-265978-i0fu8e0p	134	45	and	and	CC
cord-265978-i0fu8e0p	134	46	3.77	3.77	CD
cord-265978-i0fu8e0p	134	47	Â	â	CD
cord-265978-i0fu8e0p	134	48	10	10	CD
cord-265978-i0fu8e0p	134	49	2	2	CD
cord-265978-i0fu8e0p	134	50	to	to	IN
cord-265978-i0fu8e0p	134	51	2.88	2.88	CD
cord-265978-i0fu8e0p	134	52	Â	â	CD
cord-265978-i0fu8e0p	134	53	10	10	CD
cord-265978-i0fu8e0p	134	54	3	3	CD
cord-265978-i0fu8e0p	134	55	for	for	IN
cord-265978-i0fu8e0p	134	56	the	the	DT
cord-265978-i0fu8e0p	134	57	nasal	nasal	NN
cord-265978-i0fu8e0p	134	58	samples	sample	NNS
cord-265978-i0fu8e0p	134	59	.	.	.
cord-265978-i0fu8e0p	135	1	The	the	DT
cord-265978-i0fu8e0p	135	2	use	use	NN
cord-265978-i0fu8e0p	135	3	of	of	IN
cord-265978-i0fu8e0p	135	4	PCR	PCR	NNP
cord-265978-i0fu8e0p	135	5	in	in	IN
cord-265978-i0fu8e0p	135	6	the	the	DT
cord-265978-i0fu8e0p	135	7	field	field	NN
cord-265978-i0fu8e0p	135	8	of	of	IN
cord-265978-i0fu8e0p	135	9	virus	virus	NN
cord-265978-i0fu8e0p	135	10	disease	disease	NN
cord-265978-i0fu8e0p	135	11	diagnosis	diagnosis	NN
cord-265978-i0fu8e0p	135	12	has	have	VBZ
cord-265978-i0fu8e0p	135	13	increased	increase	VBN
cord-265978-i0fu8e0p	135	14	to	to	IN
cord-265978-i0fu8e0p	135	15	the	the	DT
cord-265978-i0fu8e0p	135	16	point	point	NN
cord-265978-i0fu8e0p	135	17	that	that	IN
cord-265978-i0fu8e0p	135	18	it	-PRON-	PRP
cord-265978-i0fu8e0p	135	19	is	be	VBZ
cord-265978-i0fu8e0p	135	20	now	now	RB
cord-265978-i0fu8e0p	135	21	accepted	accept	VBN
cord-265978-i0fu8e0p	135	22	as	as	IN
cord-265978-i0fu8e0p	135	23	the	the	DT
cord-265978-i0fu8e0p	135	24	new	new	JJ
cord-265978-i0fu8e0p	135	25	gold	gold	NN
cord-265978-i0fu8e0p	135	26	standard	standard	NN
cord-265978-i0fu8e0p	135	27	.	.	.
cord-265978-i0fu8e0p	136	1	Real	real	JJ
cord-265978-i0fu8e0p	136	2	-	-	HYPH
cord-265978-i0fu8e0p	136	3	time	time	NN
cord-265978-i0fu8e0p	136	4	PCR	PCR	NNP
cord-265978-i0fu8e0p	136	5	has	have	VBZ
cord-265978-i0fu8e0p	136	6	catalyzed	catalyze	VBN
cord-265978-i0fu8e0p	136	7	wider	wide	JJR
cord-265978-i0fu8e0p	136	8	acceptance	acceptance	NN
cord-265978-i0fu8e0p	136	9	of	of	IN
cord-265978-i0fu8e0p	136	10	PCR	PCR	NNP
cord-265978-i0fu8e0p	136	11	because	because	IN
cord-265978-i0fu8e0p	136	12	it	-PRON-	PRP
cord-265978-i0fu8e0p	136	13	is	be	VBZ
cord-265978-i0fu8e0p	136	14	more	more	RBR
cord-265978-i0fu8e0p	136	15	rapid	rapid	JJ
cord-265978-i0fu8e0p	136	16	,	,	,
cord-265978-i0fu8e0p	136	17	sensitive	sensitive	JJ
cord-265978-i0fu8e0p	136	18	and	and	CC
cord-265978-i0fu8e0p	136	19	reproducible	reproducible	JJ
cord-265978-i0fu8e0p	136	20	,	,	,
cord-265978-i0fu8e0p	136	21	while	while	IN
cord-265978-i0fu8e0p	136	22	the	the	DT
cord-265978-i0fu8e0p	136	23	risk	risk	NN
cord-265978-i0fu8e0p	136	24	of	of	IN
cord-265978-i0fu8e0p	136	25	carryover	carryover	NN
cord-265978-i0fu8e0p	136	26	contamination	contamination	NN
cord-265978-i0fu8e0p	136	27	is	be	VBZ
cord-265978-i0fu8e0p	136	28	minimized	minimize	VBN
cord-265978-i0fu8e0p	136	29	[	[	-LRB-
cord-265978-i0fu8e0p	136	30	17	17	CD
cord-265978-i0fu8e0p	136	31	]	]	-RRB-
cord-265978-i0fu8e0p	136	32	.	.	.
cord-265978-i0fu8e0p	137	1	In	in	IN
cord-265978-i0fu8e0p	137	2	the	the	DT
cord-265978-i0fu8e0p	137	3	current	current	JJ
cord-265978-i0fu8e0p	137	4	study	study	NN
cord-265978-i0fu8e0p	137	5	,	,	,
cord-265978-i0fu8e0p	137	6	the	the	DT
cord-265978-i0fu8e0p	137	7	potential	potential	NN
cord-265978-i0fu8e0p	137	8	of	of	IN
cord-265978-i0fu8e0p	137	9	real	real	JJ
cord-265978-i0fu8e0p	137	10	-	-	HYPH
cord-265978-i0fu8e0p	137	11	time	time	NN
cord-265978-i0fu8e0p	137	12	PCR	PCR	NNP
cord-265978-i0fu8e0p	137	13	was	be	VBD
cord-265978-i0fu8e0p	137	14	exploited	exploit	VBN
cord-265978-i0fu8e0p	137	15	to	to	TO
cord-265978-i0fu8e0p	137	16	develop	develop	VB
cord-265978-i0fu8e0p	137	17	a	a	DT
cord-265978-i0fu8e0p	137	18	reliable	reliable	JJ
cord-265978-i0fu8e0p	137	19	assay	assay	NN
cord-265978-i0fu8e0p	137	20	for	for	IN
cord-265978-i0fu8e0p	137	21	detection	detection	NN
cord-265978-i0fu8e0p	137	22	and	and	CC
cord-265978-i0fu8e0p	137	23	quantification	quantification	NN
cord-265978-i0fu8e0p	137	24	of	of	IN
cord-265978-i0fu8e0p	137	25	BCoV.	bcov.	CD
cord-265978-i0fu8e0p	138	1	The	the	DT
cord-265978-i0fu8e0p	138	2	success	success	NN
cord-265978-i0fu8e0p	138	3	of	of	IN
cord-265978-i0fu8e0p	138	4	any	any	DT
cord-265978-i0fu8e0p	138	5	PCR	PCR	NNP
cord-265978-i0fu8e0p	138	6	assay	assay	NN
cord-265978-i0fu8e0p	138	7	is	be	VBZ
cord-265978-i0fu8e0p	138	8	highly	highly	RB
cord-265978-i0fu8e0p	138	9	dependent	dependent	JJ
cord-265978-i0fu8e0p	138	10	on	on	IN
cord-265978-i0fu8e0p	138	11	the	the	DT
cord-265978-i0fu8e0p	138	12	primer	primer	NN
cord-265978-i0fu8e0p	138	13	design	design	NN
cord-265978-i0fu8e0p	138	14	and	and	CC
cord-265978-i0fu8e0p	138	15	its	-PRON-	PRP$
cord-265978-i0fu8e0p	138	16	ability	ability	NN
cord-265978-i0fu8e0p	138	17	to	to	TO
cord-265978-i0fu8e0p	138	18	recognize	recognize	VB
cord-265978-i0fu8e0p	138	19	all	all	DT
cord-265978-i0fu8e0p	138	20	strains	strain	NNS
cord-265978-i0fu8e0p	138	21	and	and	CC
cord-265978-i0fu8e0p	138	22	variants	variant	NNS
cord-265978-i0fu8e0p	138	23	of	of	IN
cord-265978-i0fu8e0p	138	24	the	the	DT
cord-265978-i0fu8e0p	138	25	target	target	NN
cord-265978-i0fu8e0p	138	26	organism	organism	NN
cord-265978-i0fu8e0p	138	27	.	.	.
cord-265978-i0fu8e0p	139	1	Just	just	RB
cord-265978-i0fu8e0p	139	2	as	as	RB
cord-265978-i0fu8e0p	139	3	importantly	importantly	RB
cord-265978-i0fu8e0p	139	4	,	,	,
cord-265978-i0fu8e0p	139	5	the	the	DT
cord-265978-i0fu8e0p	139	6	designed	design	VBN
cord-265978-i0fu8e0p	139	7	primers	primer	NNS
cord-265978-i0fu8e0p	139	8	should	should	MD
cord-265978-i0fu8e0p	139	9	not	not	RB
cord-265978-i0fu8e0p	139	10	cross	cross	VB
cord-265978-i0fu8e0p	139	11	react	react	VB
cord-265978-i0fu8e0p	139	12	with	with	IN
cord-265978-i0fu8e0p	139	13	other	other	JJ
cord-265978-i0fu8e0p	139	14	viruses	virus	NNS
cord-265978-i0fu8e0p	139	15	that	that	WDT
cord-265978-i0fu8e0p	139	16	may	may	MD
cord-265978-i0fu8e0p	139	17	implicate	implicate	VB
cord-265978-i0fu8e0p	139	18	in	in	IN
cord-265978-i0fu8e0p	139	19	the	the	DT
cord-265978-i0fu8e0p	139	20	same	same	JJ
cord-265978-i0fu8e0p	139	21	disease	disease	NN
cord-265978-i0fu8e0p	139	22	form(s	form(s	NNP
cord-265978-i0fu8e0p	139	23	)	)	-RRB-
cord-265978-i0fu8e0p	139	24	.	.	.
cord-265978-i0fu8e0p	140	1	The	the	DT
cord-265978-i0fu8e0p	140	2	nucleocapsid	nucleocapsid	NN
cord-265978-i0fu8e0p	140	3	(	(	-LRB-
cord-265978-i0fu8e0p	140	4	N	N	NNP
cord-265978-i0fu8e0p	140	5	)	)	-RRB-
cord-265978-i0fu8e0p	140	6	gene	gene	NN
cord-265978-i0fu8e0p	140	7	of	of	IN
cord-265978-i0fu8e0p	140	8	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	140	9	has	have	VBZ
cord-265978-i0fu8e0p	140	10	been	be	VBN
cord-265978-i0fu8e0p	140	11	shown	show	VBN
cord-265978-i0fu8e0p	140	12	to	to	TO
cord-265978-i0fu8e0p	140	13	be	be	VB
cord-265978-i0fu8e0p	140	14	highly	highly	RB
cord-265978-i0fu8e0p	140	15	conserved	conserve	VBN
cord-265978-i0fu8e0p	140	16	due	due	JJ
cord-265978-i0fu8e0p	140	17	to	to	IN
cord-265978-i0fu8e0p	140	18	strong	strong	JJ
cord-265978-i0fu8e0p	140	19	selective	selective	JJ
cord-265978-i0fu8e0p	140	20	structural	structural	JJ
cord-265978-i0fu8e0p	140	21	constraints	constraint	NNS
cord-265978-i0fu8e0p	140	22	that	that	WDT
cord-265978-i0fu8e0p	140	23	limit	limit	VBP
cord-265978-i0fu8e0p	140	24	its	-PRON-	PRP$
cord-265978-i0fu8e0p	140	25	revolution	revolution	NN
cord-265978-i0fu8e0p	140	26	[	[	-LRB-
cord-265978-i0fu8e0p	140	27	18	18	CD
cord-265978-i0fu8e0p	140	28	,	,	,
cord-265978-i0fu8e0p	140	29	19	19	CD
cord-265978-i0fu8e0p	140	30	]	]	-RRB-
cord-265978-i0fu8e0p	140	31	.	.	.
cord-265978-i0fu8e0p	141	1	Therefore	therefore	RB
cord-265978-i0fu8e0p	141	2	,	,	,
cord-265978-i0fu8e0p	141	3	a	a	DT
cord-265978-i0fu8e0p	141	4	wide	wide	JJ
cord-265978-i0fu8e0p	141	5	range	range	NN
cord-265978-i0fu8e0p	141	6	of	of	IN
cord-265978-i0fu8e0p	141	7	published	publish	VBN
cord-265978-i0fu8e0p	141	8	sequences	sequence	NNS
cord-265978-i0fu8e0p	141	9	of	of	IN
cord-265978-i0fu8e0p	141	10	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	141	11	N	n	CD
cord-265978-i0fu8e0p	141	12	gene	gene	NN
cord-265978-i0fu8e0p	141	13	were	be	VBD
cord-265978-i0fu8e0p	141	14	aligned	align	VBN
cord-265978-i0fu8e0p	141	15	together	together	RB
cord-265978-i0fu8e0p	141	16	and	and	CC
cord-265978-i0fu8e0p	141	17	against	against	IN
cord-265978-i0fu8e0p	141	18	other	other	JJ
cord-265978-i0fu8e0p	141	19	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	141	20	that	that	WDT
cord-265978-i0fu8e0p	141	21	belong	belong	VBP
cord-265978-i0fu8e0p	141	22	to	to	IN
cord-265978-i0fu8e0p	141	23	the	the	DT
cord-265978-i0fu8e0p	141	24	three	three	CD
cord-265978-i0fu8e0p	141	25	known	know	VBN
cord-265978-i0fu8e0p	141	26	antigenic	antigenic	JJ
cord-265978-i0fu8e0p	141	27	groups	group	NNS
cord-265978-i0fu8e0p	141	28	.	.	.
cord-265978-i0fu8e0p	142	1	Potential	potential	JJ
cord-265978-i0fu8e0p	142	2	target	target	NN
cord-265978-i0fu8e0p	142	3	sequences	sequence	NNS
cord-265978-i0fu8e0p	142	4	that	that	WDT
cord-265978-i0fu8e0p	142	5	were	be	VBD
cord-265978-i0fu8e0p	142	6	highly	highly	RB
cord-265978-i0fu8e0p	142	7	conserved	conserve	VBN
cord-265978-i0fu8e0p	142	8	in	in	IN
cord-265978-i0fu8e0p	142	9	different	different	JJ
cord-265978-i0fu8e0p	142	10	strains	strain	NNS
cord-265978-i0fu8e0p	142	11	of	of	IN
cord-265978-i0fu8e0p	142	12	bovine	bovine	JJ
cord-265978-i0fu8e0p	142	13	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	142	14	and	and	CC
cord-265978-i0fu8e0p	142	15	enteric	enteric	JJ
cord-265978-i0fu8e0p	142	16	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	142	17	were	be	VBD
cord-265978-i0fu8e0p	142	18	identified	identify	VBN
cord-265978-i0fu8e0p	142	19	.	.	.
cord-265978-i0fu8e0p	143	1	These	these	DT
cord-265978-i0fu8e0p	143	2	sequences	sequence	NNS
cord-265978-i0fu8e0p	143	3	,	,	,
cord-265978-i0fu8e0p	143	4	however	however	RB
cord-265978-i0fu8e0p	143	5	,	,	,
cord-265978-i0fu8e0p	143	6	share	share	VBP
cord-265978-i0fu8e0p	143	7	many	many	JJ
cord-265978-i0fu8e0p	143	8	similarities	similarity	NNS
cord-265978-i0fu8e0p	143	9	with	with	IN
cord-265978-i0fu8e0p	143	10	some	some	DT
cord-265978-i0fu8e0p	143	11	group	group	NN
cord-265978-i0fu8e0p	143	12	II	ii	CD
cord-265978-i0fu8e0p	143	13	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	143	14	that	that	WDT
cord-265978-i0fu8e0p	143	15	were	be	VBD
cord-265978-i0fu8e0p	143	16	proposed	propose	VBN
cord-265978-i0fu8e0p	143	17	to	to	TO
cord-265978-i0fu8e0p	143	18	originate	originate	VB
cord-265978-i0fu8e0p	143	19	from	from	IN
cord-265978-i0fu8e0p	143	20	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	143	21	[	[	-LRB-
cord-265978-i0fu8e0p	143	22	20e22	20e22	NNP
cord-265978-i0fu8e0p	143	23	]	]	-RRB-
cord-265978-i0fu8e0p	143	24	,	,	,
cord-265978-i0fu8e0p	143	25	a	a	DT
cord-265978-i0fu8e0p	143	26	mater	mater	NN
cord-265978-i0fu8e0p	143	27	that	that	WDT
cord-265978-i0fu8e0p	143	28	was	be	VBD
cord-265978-i0fu8e0p	143	29	unavoidable	unavoidable	JJ
cord-265978-i0fu8e0p	143	30	.	.	.
cord-265978-i0fu8e0p	144	1	More	More	JJR
cord-265978-i0fu8e0p	144	2	than	than	IN
cord-265978-i0fu8e0p	144	3	three	three	CD
cord-265978-i0fu8e0p	144	4	primer	primer	NN
cord-265978-i0fu8e0p	144	5	pairs	pair	NNS
cord-265978-i0fu8e0p	144	6	were	be	VBD
cord-265978-i0fu8e0p	144	7	synthesized	synthesize	VBN
cord-265978-i0fu8e0p	144	8	and	and	CC
cord-265978-i0fu8e0p	144	9	screened	screen	VBN
cord-265978-i0fu8e0p	144	10	for	for	IN
cord-265978-i0fu8e0p	144	11	initial	initial	JJ
cord-265978-i0fu8e0p	144	12	evaluation	evaluation	NN
cord-265978-i0fu8e0p	144	13	.	.	.
cord-265978-i0fu8e0p	145	1	Of	of	IN
cord-265978-i0fu8e0p	145	2	which	which	WDT
cord-265978-i0fu8e0p	145	3	,	,	,
cord-265978-i0fu8e0p	145	4	a	a	DT
cord-265978-i0fu8e0p	145	5	set	set	NN
cord-265978-i0fu8e0p	145	6	of	of	IN
cord-265978-i0fu8e0p	145	7	primers	primer	NNS
cord-265978-i0fu8e0p	145	8	that	that	WDT
cord-265978-i0fu8e0p	145	9	amplify	amplify	VBP
cord-265978-i0fu8e0p	145	10	a	a	DT
cord-265978-i0fu8e0p	145	11	fragment	fragment	NN
cord-265978-i0fu8e0p	145	12	of	of	IN
cord-265978-i0fu8e0p	145	13	236	236	CD
cord-265978-i0fu8e0p	145	14	bp	bp	NNP
cord-265978-i0fu8e0p	145	15	of	of	IN
cord-265978-i0fu8e0p	145	16	N	N	NNP
cord-265978-i0fu8e0p	145	17	gene	gene	NN
cord-265978-i0fu8e0p	145	18	was	be	VBD
cord-265978-i0fu8e0p	145	19	found	find	VBN
cord-265978-i0fu8e0p	145	20	to	to	TO
cord-265978-i0fu8e0p	145	21	be	be	VB
cord-265978-i0fu8e0p	145	22	most	most	RBS
cord-265978-i0fu8e0p	145	23	sensitive	sensitive	JJ
cord-265978-i0fu8e0p	145	24	(	(	-LRB-
cord-265978-i0fu8e0p	145	25	Table	table	NN
cord-265978-i0fu8e0p	145	26	2	2	CD
cord-265978-i0fu8e0p	145	27	)	)	-RRB-
cord-265978-i0fu8e0p	145	28	.	.	.
cord-265978-i0fu8e0p	146	1	In	in	IN
cord-265978-i0fu8e0p	146	2	addition	addition	NN
cord-265978-i0fu8e0p	146	3	to	to	IN
cord-265978-i0fu8e0p	146	4	the	the	DT
cord-265978-i0fu8e0p	146	5	choice	choice	NN
cord-265978-i0fu8e0p	146	6	of	of	IN
cord-265978-i0fu8e0p	146	7	target	target	NN
cord-265978-i0fu8e0p	146	8	region	region	NN
cord-265978-i0fu8e0p	146	9	,	,	,
cord-265978-i0fu8e0p	146	10	the	the	DT
cord-265978-i0fu8e0p	146	11	design	design	NN
cord-265978-i0fu8e0p	146	12	of	of	IN
cord-265978-i0fu8e0p	146	13	"	"	``
cord-265978-i0fu8e0p	146	14	inhouse	inhouse	JJ
cord-265978-i0fu8e0p	146	15	"	"	''
cord-265978-i0fu8e0p	146	16	real	real	JJ
cord-265978-i0fu8e0p	146	17	-	-	HYPH
cord-265978-i0fu8e0p	146	18	time	time	NN
cord-265978-i0fu8e0p	146	19	PCR	PCR	NNP
cord-265978-i0fu8e0p	146	20	protocols	protocol	NNS
cord-265978-i0fu8e0p	146	21	requires	require	VBZ
cord-265978-i0fu8e0p	146	22	the	the	DT
cord-265978-i0fu8e0p	146	23	optimization	optimization	NN
cord-265978-i0fu8e0p	146	24	of	of	IN
cord-265978-i0fu8e0p	146	25	several	several	JJ
cord-265978-i0fu8e0p	146	26	amplification	amplification	NN
cord-265978-i0fu8e0p	146	27	conditions	condition	NNS
cord-265978-i0fu8e0p	146	28	.	.	.
cord-265978-i0fu8e0p	147	1	The	the	DT
cord-265978-i0fu8e0p	147	2	key	key	JJ
cord-265978-i0fu8e0p	147	3	elements	element	NNS
cord-265978-i0fu8e0p	147	4	of	of	IN
cord-265978-i0fu8e0p	147	5	such	such	JJ
cord-265978-i0fu8e0p	147	6	optimization	optimization	NN
cord-265978-i0fu8e0p	147	7	include	include	VBP
cord-265978-i0fu8e0p	147	8	;	;	:
cord-265978-i0fu8e0p	147	9	the	the	DT
cord-265978-i0fu8e0p	147	10	magnesium	magnesium	NN
cord-265978-i0fu8e0p	147	11	chloride	chloride	NN
cord-265978-i0fu8e0p	147	12	concentration	concentration	NN
cord-265978-i0fu8e0p	147	13	,	,	,
cord-265978-i0fu8e0p	147	14	which	which	WDT
cord-265978-i0fu8e0p	147	15	allows	allow	VBZ
cord-265978-i0fu8e0p	147	16	the	the	DT
cord-265978-i0fu8e0p	147	17	polymerase	polymerase	NN
cord-265978-i0fu8e0p	147	18	enzyme	enzyme	NN
cord-265978-i0fu8e0p	147	19	to	to	TO
cord-265978-i0fu8e0p	147	20	function	function	VB
cord-265978-i0fu8e0p	147	21	at	at	IN
cord-265978-i0fu8e0p	147	22	an	an	DT
cord-265978-i0fu8e0p	147	23	optimal	optimal	JJ
cord-265978-i0fu8e0p	147	24	level	level	NN
cord-265978-i0fu8e0p	147	25	,	,	,
cord-265978-i0fu8e0p	147	26	the	the	DT
cord-265978-i0fu8e0p	147	27	concentration	concentration	NN
cord-265978-i0fu8e0p	147	28	of	of	IN
cord-265978-i0fu8e0p	147	29	different	different	JJ
cord-265978-i0fu8e0p	147	30	primers	primer	NNS
cord-265978-i0fu8e0p	147	31	used	use	VBN
cord-265978-i0fu8e0p	147	32	,	,	,
cord-265978-i0fu8e0p	147	33	which	which	WDT
cord-265978-i0fu8e0p	147	34	affect	affect	VBP
cord-265978-i0fu8e0p	147	35	the	the	DT
cord-265978-i0fu8e0p	147	36	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	147	37	and	and	CC
cord-265978-i0fu8e0p	147	38	linearity	linearity	NN
cord-265978-i0fu8e0p	147	39	of	of	IN
cord-265978-i0fu8e0p	147	40	the	the	DT
cord-265978-i0fu8e0p	147	41	assay	assay	NN
cord-265978-i0fu8e0p	147	42	,	,	,
cord-265978-i0fu8e0p	147	43	and	and	CC
cord-265978-i0fu8e0p	147	44	the	the	DT
cord-265978-i0fu8e0p	147	45	temperature	temperature	NN
cord-265978-i0fu8e0p	147	46	of	of	IN
cord-265978-i0fu8e0p	147	47	primer	primer	NN
cord-265978-i0fu8e0p	147	48	annealing	annealing	NN
cord-265978-i0fu8e0p	147	49	,	,	,
cord-265978-i0fu8e0p	147	50	which	which	WDT
cord-265978-i0fu8e0p	147	51	affects	affect	VBZ
cord-265978-i0fu8e0p	147	52	the	the	DT
cord-265978-i0fu8e0p	147	53	specificity	specificity	NN
cord-265978-i0fu8e0p	147	54	of	of	IN
cord-265978-i0fu8e0p	147	55	the	the	DT
cord-265978-i0fu8e0p	147	56	reaction	reaction	NN
cord-265978-i0fu8e0p	147	57	[	[	-LRB-
cord-265978-i0fu8e0p	147	58	23	23	CD
cord-265978-i0fu8e0p	147	59	,	,	,
cord-265978-i0fu8e0p	147	60	24	24	CD
cord-265978-i0fu8e0p	147	61	]	]	-RRB-
cord-265978-i0fu8e0p	147	62	.	.	.
cord-265978-i0fu8e0p	148	1	The	the	DT
cord-265978-i0fu8e0p	148	2	current	current	JJ
cord-265978-i0fu8e0p	148	3	assay	assay	NN
cord-265978-i0fu8e0p	148	4	was	be	VBD
cord-265978-i0fu8e0p	148	5	optimized	optimize	VBN
cord-265978-i0fu8e0p	148	6	using	use	VBG
cord-265978-i0fu8e0p	148	7	the	the	DT
cord-265978-i0fu8e0p	148	8	cell	cell	NN
cord-265978-i0fu8e0p	148	9	-	-	HYPH
cord-265978-i0fu8e0p	148	10	culture	culture	NN
cord-265978-i0fu8e0p	148	11	grown	grow	VBN
cord-265978-i0fu8e0p	148	12	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	148	13	,	,	,
cord-265978-i0fu8e0p	148	14	strain	strain	VB
cord-265978-i0fu8e0p	148	15	Mebus	Mebus	NNP
cord-265978-i0fu8e0p	148	16	,	,	,
cord-265978-i0fu8e0p	148	17	by	by	IN
cord-265978-i0fu8e0p	148	18	varying	vary	VBG
cord-265978-i0fu8e0p	148	19	each	each	DT
cord-265978-i0fu8e0p	148	20	of	of	IN
cord-265978-i0fu8e0p	148	21	these	these	DT
cord-265978-i0fu8e0p	148	22	parameters	parameter	NNS
cord-265978-i0fu8e0p	148	23	individually	individually	RB
cord-265978-i0fu8e0p	148	24	.	.	.
cord-265978-i0fu8e0p	149	1	The	the	DT
cord-265978-i0fu8e0p	149	2	optimal	optimal	JJ
cord-265978-i0fu8e0p	149	3	amplification	amplification	NN
cord-265978-i0fu8e0p	149	4	conditions	condition	NNS
cord-265978-i0fu8e0p	149	5	were	be	VBD
cord-265978-i0fu8e0p	149	6	assessed	assess	VBN
cord-265978-i0fu8e0p	149	7	by	by	IN
cord-265978-i0fu8e0p	149	8	reporting	report	VBG
cord-265978-i0fu8e0p	149	9	the	the	DT
cord-265978-i0fu8e0p	149	10	fluorescence	fluorescence	NN
cord-265978-i0fu8e0p	149	11	intensity	intensity	NN
cord-265978-i0fu8e0p	149	12	and	and	CC
cord-265978-i0fu8e0p	149	13	the	the	DT
cord-265978-i0fu8e0p	149	14	value	value	NN
cord-265978-i0fu8e0p	149	15	of	of	IN
cord-265978-i0fu8e0p	149	16	threshold	threshold	NN
cord-265978-i0fu8e0p	149	17	cycles	cycle	NNS
cord-265978-i0fu8e0p	149	18	(	(	-LRB-
cord-265978-i0fu8e0p	149	19	C	C	NNP
cord-265978-i0fu8e0p	149	20	T	T	NNP
cord-265978-i0fu8e0p	149	21	)	)	-RRB-
cord-265978-i0fu8e0p	149	22	.	.	.
cord-265978-i0fu8e0p	150	1	The	the	DT
cord-265978-i0fu8e0p	150	2	use	use	NN
cord-265978-i0fu8e0p	150	3	of	of	IN
cord-265978-i0fu8e0p	150	4	fecal	fecal	JJ
cord-265978-i0fu8e0p	150	5	and	and	CC
cord-265978-i0fu8e0p	150	6	nasal	nasal	NN
cord-265978-i0fu8e0p	150	7	swab	swab	NN
cord-265978-i0fu8e0p	150	8	samples	sample	NNS
cord-265978-i0fu8e0p	150	9	as	as	IN
cord-265978-i0fu8e0p	150	10	the	the	DT
cord-265978-i0fu8e0p	150	11	most	most	RBS
cord-265978-i0fu8e0p	150	12	frequent	frequent	JJ
cord-265978-i0fu8e0p	150	13	matrices	matrix	NNS
cord-265978-i0fu8e0p	150	14	for	for	IN
cord-265978-i0fu8e0p	150	15	detection	detection	NN
cord-265978-i0fu8e0p	150	16	of	of	IN
cord-265978-i0fu8e0p	150	17	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	150	18	in	in	IN
cord-265978-i0fu8e0p	150	19	diseased	diseased	JJ
cord-265978-i0fu8e0p	150	20	animals	animal	NNS
cord-265978-i0fu8e0p	150	21	constitutes	constitute	VBZ
cord-265978-i0fu8e0p	150	22	one	one	CD
cord-265978-i0fu8e0p	150	23	of	of	IN
cord-265978-i0fu8e0p	150	24	the	the	DT
cord-265978-i0fu8e0p	150	25	significant	significant	JJ
cord-265978-i0fu8e0p	150	26	problems	problem	NNS
cord-265978-i0fu8e0p	150	27	that	that	WDT
cord-265978-i0fu8e0p	150	28	may	may	MD
cord-265978-i0fu8e0p	150	29	hinder	hinder	VB
cord-265978-i0fu8e0p	150	30	the	the	DT
cord-265978-i0fu8e0p	150	31	performance	performance	NN
cord-265978-i0fu8e0p	150	32	of	of	IN
cord-265978-i0fu8e0p	150	33	RT	RT	NNP
cord-265978-i0fu8e0p	150	34	-	-	HYPH
cord-265978-i0fu8e0p	150	35	PCR	PCR	NNP
cord-265978-i0fu8e0p	150	36	assays	assay	NNS
cord-265978-i0fu8e0p	150	37	.	.	.
cord-265978-i0fu8e0p	151	1	Considerable	considerable	JJ
cord-265978-i0fu8e0p	151	2	existence	existence	NN
cord-265978-i0fu8e0p	151	3	of	of	IN
cord-265978-i0fu8e0p	151	4	the	the	DT
cord-265978-i0fu8e0p	151	5	bile	bile	NN
cord-265978-i0fu8e0p	151	6	salts	salt	NNS
cord-265978-i0fu8e0p	151	7	and	and	CC
cord-265978-i0fu8e0p	151	8	the	the	DT
cord-265978-i0fu8e0p	151	9	complex	complex	JJ
cord-265978-i0fu8e0p	151	10	polysaccharides	polysaccharide	NNS
cord-265978-i0fu8e0p	151	11	in	in	IN
cord-265978-i0fu8e0p	151	12	these	these	DT
cord-265978-i0fu8e0p	151	13	matrices	matrix	NNS
cord-265978-i0fu8e0p	151	14	can	can	MD
cord-265978-i0fu8e0p	151	15	either	either	CC
cord-265978-i0fu8e0p	151	16	block	block	VB
cord-265978-i0fu8e0p	151	17	the	the	DT
cord-265978-i0fu8e0p	151	18	enzyme	enzyme	NN
cord-265978-i0fu8e0p	151	19	activity	activity	NN
cord-265978-i0fu8e0p	151	20	or	or	CC
cord-265978-i0fu8e0p	151	21	reduce	reduce	VB
cord-265978-i0fu8e0p	151	22	the	the	DT
cord-265978-i0fu8e0p	151	23	Magnesium	magnesium	NN
cord-265978-i0fu8e0p	151	24	salt	salt	NN
cord-265978-i0fu8e0p	151	25	availability	availability	NN
cord-265978-i0fu8e0p	151	26	[	[	-LRB-
cord-265978-i0fu8e0p	151	27	25	25	CD
cord-265978-i0fu8e0p	151	28	]	]	-RRB-
cord-265978-i0fu8e0p	151	29	.	.	.
cord-265978-i0fu8e0p	152	1	Therefore	therefore	RB
cord-265978-i0fu8e0p	152	2	,	,	,
cord-265978-i0fu8e0p	152	3	monitoring	monitor	VBG
cord-265978-i0fu8e0p	152	4	PCR	PCR	NNP
cord-265978-i0fu8e0p	152	5	inhibitors	inhibitor	NNS
cord-265978-i0fu8e0p	152	6	in	in	IN
cord-265978-i0fu8e0p	152	7	the	the	DT
cord-265978-i0fu8e0p	152	8	utilized	utilize	VBN
cord-265978-i0fu8e0p	152	9	samples	sample	NNS
cord-265978-i0fu8e0p	152	10	is	be	VBZ
cord-265978-i0fu8e0p	152	11	very	very	RB
cord-265978-i0fu8e0p	152	12	important	important	JJ
cord-265978-i0fu8e0p	152	13	to	to	TO
cord-265978-i0fu8e0p	152	14	avoid	avoid	VB
cord-265978-i0fu8e0p	152	15	false	false	JJ
cord-265978-i0fu8e0p	152	16	negative	negative	JJ
cord-265978-i0fu8e0p	152	17	results	result	NNS
cord-265978-i0fu8e0p	152	18	.	.	.
cord-265978-i0fu8e0p	153	1	One	one	CD
cord-265978-i0fu8e0p	153	2	of	of	IN
cord-265978-i0fu8e0p	153	3	the	the	DT
cord-265978-i0fu8e0p	153	4	most	most	RBS
cord-265978-i0fu8e0p	153	5	important	important	JJ
cord-265978-i0fu8e0p	153	6	methods	method	NNS
cord-265978-i0fu8e0p	153	7	for	for	IN
cord-265978-i0fu8e0p	153	8	monitoring	monitor	VBG
cord-265978-i0fu8e0p	153	9	PCR	PCR	NNP
cord-265978-i0fu8e0p	153	10	inhibitors	inhibitor	NNS
cord-265978-i0fu8e0p	153	11	is	be	VBZ
cord-265978-i0fu8e0p	153	12	the	the	DT
cord-265978-i0fu8e0p	153	13	inclusion	inclusion	NN
cord-265978-i0fu8e0p	153	14	and/or	and/or	CC
cord-265978-i0fu8e0p	153	15	detection	detection	NN
cord-265978-i0fu8e0p	153	16	of	of	IN
cord-265978-i0fu8e0p	153	17	an	an	DT
cord-265978-i0fu8e0p	153	18	internal	internal	JJ
cord-265978-i0fu8e0p	153	19	positive	positive	JJ
cord-265978-i0fu8e0p	153	20	control	control	NN
cord-265978-i0fu8e0p	153	21	(	(	-LRB-
cord-265978-i0fu8e0p	153	22	IPC	IPC	NNP
cord-265978-i0fu8e0p	153	23	)	)	-RRB-
cord-265978-i0fu8e0p	153	24	in	in	IN
cord-265978-i0fu8e0p	153	25	the	the	DT
cord-265978-i0fu8e0p	153	26	test	test	NN
cord-265978-i0fu8e0p	153	27	samples	sample	NNS
cord-265978-i0fu8e0p	153	28	[	[	-LRB-
cord-265978-i0fu8e0p	153	29	26	26	CD
cord-265978-i0fu8e0p	153	30	]	]	-RRB-
cord-265978-i0fu8e0p	153	31	.	.	.
cord-265978-i0fu8e0p	154	1	The	the	DT
cord-265978-i0fu8e0p	154	2	added	add	VBN
cord-265978-i0fu8e0p	154	3	advantage	advantage	NN
cord-265978-i0fu8e0p	154	4	of	of	IN
cord-265978-i0fu8e0p	154	5	using	use	VBG
cord-265978-i0fu8e0p	154	6	an	an	DT
cord-265978-i0fu8e0p	154	7	IPC	IPC	NNP
cord-265978-i0fu8e0p	154	8	is	be	VBZ
cord-265978-i0fu8e0p	154	9	the	the	DT
cord-265978-i0fu8e0p	154	10	ability	ability	NN
cord-265978-i0fu8e0p	154	11	to	to	TO
cord-265978-i0fu8e0p	154	12	control	control	VB
cord-265978-i0fu8e0p	154	13	and	and	CC
cord-265978-i0fu8e0p	154	14	normalize	normalize	VB
cord-265978-i0fu8e0p	154	15	the	the	DT
cord-265978-i0fu8e0p	154	16	variable	variable	JJ
cord-265978-i0fu8e0p	154	17	conditions	condition	NNS
cord-265978-i0fu8e0p	154	18	of	of	IN
cord-265978-i0fu8e0p	154	19	the	the	DT
cord-265978-i0fu8e0p	154	20	assay	assay	NN
cord-265978-i0fu8e0p	154	21	starting	start	VBG
cord-265978-i0fu8e0p	154	22	from	from	IN
cord-265978-i0fu8e0p	154	23	the	the	DT
cord-265978-i0fu8e0p	154	24	efficiency	efficiency	NN
cord-265978-i0fu8e0p	154	25	of	of	IN
cord-265978-i0fu8e0p	154	26	sample	sample	NN
cord-265978-i0fu8e0p	154	27	extraction	extraction	NN
cord-265978-i0fu8e0p	154	28	and	and	CC
cord-265978-i0fu8e0p	154	29	ending	end	VBG
cord-265978-i0fu8e0p	154	30	with	with	IN
cord-265978-i0fu8e0p	154	31	the	the	DT
cord-265978-i0fu8e0p	154	32	real	real	JJ
cord-265978-i0fu8e0p	154	33	-	-	HYPH
cord-265978-i0fu8e0p	154	34	time	time	NN
cord-265978-i0fu8e0p	154	35	amplification/	amplification/	CD
cord-265978-i0fu8e0p	154	36	detection	detection	NN
cord-265978-i0fu8e0p	154	37	.	.	.
cord-265978-i0fu8e0p	155	1	In	in	IN
cord-265978-i0fu8e0p	155	2	the	the	DT
cord-265978-i0fu8e0p	155	3	process	process	NN
cord-265978-i0fu8e0p	155	4	of	of	IN
cord-265978-i0fu8e0p	155	5	developing	develop	VBG
cord-265978-i0fu8e0p	155	6	the	the	DT
cord-265978-i0fu8e0p	155	7	present	present	JJ
cord-265978-i0fu8e0p	155	8	assay	assay	NN
cord-265978-i0fu8e0p	155	9	,	,	,
cord-265978-i0fu8e0p	155	10	a	a	DT
cord-265978-i0fu8e0p	155	11	primer	primer	NN
cord-265978-i0fu8e0p	155	12	set	set	VBN
cord-265978-i0fu8e0p	155	13	specific	specific	JJ
cord-265978-i0fu8e0p	155	14	for	for	IN
cord-265978-i0fu8e0p	155	15	the	the	DT
cord-265978-i0fu8e0p	155	16	house	house	NN
cord-265978-i0fu8e0p	155	17	keeping	keep	VBG
cord-265978-i0fu8e0p	155	18	gene	gene	NN
cord-265978-i0fu8e0p	155	19	"	"	``
cord-265978-i0fu8e0p	155	20	Glyceraldehyde-3-phosphate	glyceraldehyde-3-phosphate	NN
cord-265978-i0fu8e0p	155	21	dehydrogenase	dehydrogenase	NN
cord-265978-i0fu8e0p	155	22	(	(	-LRB-
cord-265978-i0fu8e0p	155	23	GAPDH	GAPDH	NNP
cord-265978-i0fu8e0p	155	24	)	)	-RRB-
cord-265978-i0fu8e0p	155	25	"	"	''
cord-265978-i0fu8e0p	155	26	of	of	IN
cord-265978-i0fu8e0p	155	27	bovine	bovine	JJ
cord-265978-i0fu8e0p	155	28	species	specie	NNS
cord-265978-i0fu8e0p	155	29	was	be	VBD
cord-265978-i0fu8e0p	155	30	included	include	VBN
cord-265978-i0fu8e0p	155	31	in	in	IN
cord-265978-i0fu8e0p	155	32	the	the	DT
cord-265978-i0fu8e0p	155	33	reaction	reaction	NN
cord-265978-i0fu8e0p	155	34	.	.	.
cord-265978-i0fu8e0p	156	1	Specific	specific	JJ
cord-265978-i0fu8e0p	156	2	identification	identification	NN
cord-265978-i0fu8e0p	156	3	of	of	IN
cord-265978-i0fu8e0p	156	4	the	the	DT
cord-265978-i0fu8e0p	156	5	IPC	IPC	NNP
cord-265978-i0fu8e0p	156	6	product	product	NN
cord-265978-i0fu8e0p	156	7	by	by	IN
cord-265978-i0fu8e0p	156	8	melt	melt	NN
cord-265978-i0fu8e0p	156	9	curve	curve	NN
cord-265978-i0fu8e0p	156	10	analysis	analysis	NN
cord-265978-i0fu8e0p	156	11	revealed	reveal	VBD
cord-265978-i0fu8e0p	156	12	a	a	DT
cord-265978-i0fu8e0p	156	13	clearly	clearly	RB
cord-265978-i0fu8e0p	156	14	distinguished	distinguished	JJ
cord-265978-i0fu8e0p	156	15	melt	melt	NN
cord-265978-i0fu8e0p	156	16	peak	peak	NN
cord-265978-i0fu8e0p	156	17	of	of	IN
cord-265978-i0fu8e0p	156	18	82.54	82.54	CD
cord-265978-i0fu8e0p	156	19	AE	AE	NNP
cord-265978-i0fu8e0p	156	20	0.32	0.32	CD
cord-265978-i0fu8e0p	156	21	C.	C.	NNP
cord-265978-i0fu8e0p	156	22	Analysis	Analysis	NNP
cord-265978-i0fu8e0p	156	23	of	of	IN
cord-265978-i0fu8e0p	156	24	variance	variance	NN
cord-265978-i0fu8e0p	156	25	revealed	reveal	VBD
cord-265978-i0fu8e0p	156	26	no	no	DT
cord-265978-i0fu8e0p	156	27	significant	significant	JJ
cord-265978-i0fu8e0p	156	28	differences	difference	NNS
cord-265978-i0fu8e0p	156	29	in	in	IN
cord-265978-i0fu8e0p	156	30	the	the	DT
cord-265978-i0fu8e0p	156	31	assay	assay	NN
cord-265978-i0fu8e0p	156	32	performance	performance	NN
cord-265978-i0fu8e0p	156	33	between	between	IN
cord-265978-i0fu8e0p	156	34	monoplex	monoplex	NNP
cord-265978-i0fu8e0p	156	35	and	and	CC
cord-265978-i0fu8e0p	156	36	duplex	duplex	NN
cord-265978-i0fu8e0p	156	37	reactions	reaction	NNS
cord-265978-i0fu8e0p	156	38	in	in	IN
cord-265978-i0fu8e0p	156	39	terms	term	NNS
cord-265978-i0fu8e0p	156	40	of	of	IN
cord-265978-i0fu8e0p	156	41	specificity	specificity	NN
cord-265978-i0fu8e0p	156	42	,	,	,
cord-265978-i0fu8e0p	156	43	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	156	44	and	and	CC
cord-265978-i0fu8e0p	156	45	linearity	linearity	NN
cord-265978-i0fu8e0p	156	46	(	(	-LRB-
cord-265978-i0fu8e0p	156	47	data	datum	NNS
cord-265978-i0fu8e0p	156	48	not	not	RB
cord-265978-i0fu8e0p	156	49	shown	show	VBN
cord-265978-i0fu8e0p	156	50	)	)	-RRB-
cord-265978-i0fu8e0p	156	51	.	.	.
cord-265978-i0fu8e0p	157	1	This	this	DT
cord-265978-i0fu8e0p	157	2	implies	imply	VBZ
cord-265978-i0fu8e0p	157	3	that	that	IN
cord-265978-i0fu8e0p	157	4	co	co	NN
cord-265978-i0fu8e0p	157	5	-	-	NN
cord-265978-i0fu8e0p	157	6	amplification	amplification	NN
cord-265978-i0fu8e0p	157	7	of	of	IN
cord-265978-i0fu8e0p	157	8	the	the	DT
cord-265978-i0fu8e0p	157	9	IPC	IPC	NNP
cord-265978-i0fu8e0p	157	10	does	do	VBZ
cord-265978-i0fu8e0p	157	11	not	not	RB
cord-265978-i0fu8e0p	157	12	affect	affect	VB
cord-265978-i0fu8e0p	157	13	the	the	DT
cord-265978-i0fu8e0p	157	14	detection	detection	NN
cord-265978-i0fu8e0p	157	15	and	and	CC
cord-265978-i0fu8e0p	157	16	quantification	quantification	NN
cord-265978-i0fu8e0p	157	17	of	of	IN
cord-265978-i0fu8e0p	157	18	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	157	19	RNA	RNA	NNP
cord-265978-i0fu8e0p	157	20	.	.	.
cord-265978-i0fu8e0p	158	1	Validation	validation	NN
cord-265978-i0fu8e0p	158	2	of	of	IN
cord-265978-i0fu8e0p	158	3	'	'	''
cord-265978-i0fu8e0p	158	4	in	in	IN
cord-265978-i0fu8e0p	158	5	-	-	HYPH
cord-265978-i0fu8e0p	158	6	house	house	NN
cord-265978-i0fu8e0p	158	7	'	'	''
cord-265978-i0fu8e0p	158	8	real	real	JJ
cord-265978-i0fu8e0p	158	9	-	-	HYPH
cord-265978-i0fu8e0p	158	10	time	time	NN
cord-265978-i0fu8e0p	158	11	PCR	PCR	NNP
cord-265978-i0fu8e0p	158	12	assays	assay	NNS
cord-265978-i0fu8e0p	158	13	is	be	VBZ
cord-265978-i0fu8e0p	158	14	a	a	DT
cord-265978-i0fu8e0p	158	15	crucial	crucial	JJ
cord-265978-i0fu8e0p	158	16	aspect	aspect	NN
cord-265978-i0fu8e0p	158	17	that	that	WDT
cord-265978-i0fu8e0p	158	18	controls	control	VBZ
cord-265978-i0fu8e0p	158	19	the	the	DT
cord-265978-i0fu8e0p	158	20	applicability	applicability	NN
cord-265978-i0fu8e0p	158	21	of	of	IN
cord-265978-i0fu8e0p	158	22	these	these	DT
cord-265978-i0fu8e0p	158	23	assays	assay	NNS
cord-265978-i0fu8e0p	158	24	in	in	IN
cord-265978-i0fu8e0p	158	25	clinical	clinical	JJ
cord-265978-i0fu8e0p	158	26	and	and	CC
cord-265978-i0fu8e0p	158	27	diagnostic	diagnostic	JJ
cord-265978-i0fu8e0p	158	28	purposes	purpose	NNS
cord-265978-i0fu8e0p	158	29	.	.	.
cord-265978-i0fu8e0p	159	1	Four	four	CD
cord-265978-i0fu8e0p	159	2	cardinal	cardinal	JJ
cord-265978-i0fu8e0p	159	3	elements	element	NNS
cord-265978-i0fu8e0p	159	4	of	of	IN
cord-265978-i0fu8e0p	159	5	validation	validation	NN
cord-265978-i0fu8e0p	159	6	were	be	VBD
cord-265978-i0fu8e0p	159	7	proposed	propose	VBN
cord-265978-i0fu8e0p	159	8	including	include	VBG
cord-265978-i0fu8e0p	159	9	specificity	specificity	NN
cord-265978-i0fu8e0p	159	10	,	,	,
cord-265978-i0fu8e0p	159	11	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	159	12	,	,	,
cord-265978-i0fu8e0p	159	13	reproducibility	reproducibility	NN
cord-265978-i0fu8e0p	159	14	and	and	CC
cord-265978-i0fu8e0p	159	15	linearity	linearity	NN
cord-265978-i0fu8e0p	159	16	[	[	-LRB-
cord-265978-i0fu8e0p	159	17	27	27	CD
cord-265978-i0fu8e0p	159	18	]	]	-RRB-
cord-265978-i0fu8e0p	159	19	.	.	.
cord-265978-i0fu8e0p	160	1	In	in	IN
cord-265978-i0fu8e0p	160	2	our	-PRON-	PRP$
cord-265978-i0fu8e0p	160	3	assay	assay	NN
cord-265978-i0fu8e0p	160	4	,	,	,
cord-265978-i0fu8e0p	160	5	all	all	PDT
cord-265978-i0fu8e0p	160	6	these	these	DT
cord-265978-i0fu8e0p	160	7	parameters	parameter	NNS
cord-265978-i0fu8e0p	160	8	were	be	VBD
cord-265978-i0fu8e0p	160	9	considered	consider	VBN
cord-265978-i0fu8e0p	160	10	for	for	IN
cord-265978-i0fu8e0p	160	11	evaluation	evaluation	NN
cord-265978-i0fu8e0p	160	12	.	.	.
cord-265978-i0fu8e0p	161	1	The	the	DT
cord-265978-i0fu8e0p	161	2	specificity	specificity	NN
cord-265978-i0fu8e0p	161	3	of	of	IN
cord-265978-i0fu8e0p	161	4	the	the	DT
cord-265978-i0fu8e0p	161	5	reaction	reaction	NN
cord-265978-i0fu8e0p	161	6	has	have	VBZ
cord-265978-i0fu8e0p	161	7	been	be	VBN
cord-265978-i0fu8e0p	161	8	proved	prove	VBN
cord-265978-i0fu8e0p	161	9	by	by	IN
cord-265978-i0fu8e0p	161	10	different	different	JJ
cord-265978-i0fu8e0p	161	11	means	mean	NNS
cord-265978-i0fu8e0p	161	12	including	include	VBG
cord-265978-i0fu8e0p	161	13	;	;	:
cord-265978-i0fu8e0p	161	14	a	a	LS
cord-265978-i0fu8e0p	161	15	)	)	-RRB-
cord-265978-i0fu8e0p	161	16	identification	identification	NN
cord-265978-i0fu8e0p	161	17	of	of	IN
cord-265978-i0fu8e0p	161	18	the	the	DT
cord-265978-i0fu8e0p	161	19	amplificon	amplificon	NNP
cord-265978-i0fu8e0p	161	20	as	as	IN
cord-265978-i0fu8e0p	161	21	a	a	DT
cord-265978-i0fu8e0p	161	22	distinct	distinct	JJ
cord-265978-i0fu8e0p	161	23	band	band	NN
cord-265978-i0fu8e0p	161	24	of	of	IN
cord-265978-i0fu8e0p	161	25	the	the	DT
cord-265978-i0fu8e0p	161	26	expected	expect	VBN
cord-265978-i0fu8e0p	161	27	molecular	molecular	JJ
cord-265978-i0fu8e0p	161	28	size	size	NN
cord-265978-i0fu8e0p	161	29	by	by	IN
cord-265978-i0fu8e0p	161	30	agarose	agarose	JJ
cord-265978-i0fu8e0p	161	31	gel	gel	NN
cord-265978-i0fu8e0p	161	32	electrophoresis	electrophoresis	NN
cord-265978-i0fu8e0p	161	33	,	,	,
cord-265978-i0fu8e0p	161	34	b	b	LS
cord-265978-i0fu8e0p	161	35	)	)	-RRB-
cord-265978-i0fu8e0p	161	36	generation	generation	NN
cord-265978-i0fu8e0p	161	37	of	of	IN
cord-265978-i0fu8e0p	161	38	a	a	DT
cord-265978-i0fu8e0p	161	39	consistent	consistent	JJ
cord-265978-i0fu8e0p	161	40	melt	melt	NN
cord-265978-i0fu8e0p	161	41	peak	peak	NN
cord-265978-i0fu8e0p	161	42	at	at	IN
cord-265978-i0fu8e0p	161	43	78.35	78.35	CD
cord-265978-i0fu8e0p	161	44	C	c	NN
cord-265978-i0fu8e0p	161	45	AE	AE	NNP
cord-265978-i0fu8e0p	161	46	0.26	0.26	CD
cord-265978-i0fu8e0p	161	47	by	by	IN
cord-265978-i0fu8e0p	161	48	melt	melt	NN
cord-265978-i0fu8e0p	161	49	curve	curve	NN
cord-265978-i0fu8e0p	161	50	analysis	analysis	NN
cord-265978-i0fu8e0p	161	51	,	,	,
cord-265978-i0fu8e0p	161	52	c	c	NNP
cord-265978-i0fu8e0p	161	53	)	)	-RRB-
cord-265978-i0fu8e0p	161	54	lack	lack	NN
cord-265978-i0fu8e0p	161	55	of	of	IN
cord-265978-i0fu8e0p	161	56	cross	cross	NN
cord-265978-i0fu8e0p	161	57	reactivity	reactivity	NN
cord-265978-i0fu8e0p	161	58	with	with	IN
cord-265978-i0fu8e0p	161	59	other	other	JJ
cord-265978-i0fu8e0p	161	60	bovine	bovine	JJ
cord-265978-i0fu8e0p	161	61	viruses	virus	NNS
cord-265978-i0fu8e0p	161	62	that	that	WDT
cord-265978-i0fu8e0p	161	63	may	may	MD
cord-265978-i0fu8e0p	161	64	be	be	VB
cord-265978-i0fu8e0p	161	65	considered	consider	VBN
cord-265978-i0fu8e0p	161	66	for	for	IN
cord-265978-i0fu8e0p	161	67	differential	differential	JJ
cord-265978-i0fu8e0p	161	68	diagnosis	diagnosis	NN
cord-265978-i0fu8e0p	161	69	with	with	IN
cord-265978-i0fu8e0p	161	70	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	161	71	,	,	,
cord-265978-i0fu8e0p	161	72	d	d	LS
cord-265978-i0fu8e0p	161	73	)	)	-RRB-
cord-265978-i0fu8e0p	161	74	Lack	lack	NN
cord-265978-i0fu8e0p	161	75	of	of	IN
cord-265978-i0fu8e0p	161	76	cross	cross	NN
cord-265978-i0fu8e0p	161	77	reactivity	reactivity	NN
cord-265978-i0fu8e0p	161	78	with	with	IN
cord-265978-i0fu8e0p	161	79	a	a	DT
cord-265978-i0fu8e0p	161	80	representative	representative	JJ
cord-265978-i0fu8e0p	161	81	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	161	82	(	(	-LRB-
cord-265978-i0fu8e0p	161	83	IBV	IBV	NNP
cord-265978-i0fu8e0p	161	84	)	)	-RRB-
cord-265978-i0fu8e0p	161	85	,	,	,
cord-265978-i0fu8e0p	161	86	and	and	CC
cord-265978-i0fu8e0p	161	87	e	e	LS
cord-265978-i0fu8e0p	161	88	)	)	-RRB-
cord-265978-i0fu8e0p	161	89	Absence	absence	NN
cord-265978-i0fu8e0p	161	90	of	of	IN
cord-265978-i0fu8e0p	161	91	regular	regular	JJ
cord-265978-i0fu8e0p	161	92	amplification	amplification	NN
cord-265978-i0fu8e0p	161	93	signals	signal	NNS
cord-265978-i0fu8e0p	161	94	in	in	IN
cord-265978-i0fu8e0p	161	95	different	different	JJ
cord-265978-i0fu8e0p	161	96	types	type	NNS
cord-265978-i0fu8e0p	161	97	of	of	IN
cord-265978-i0fu8e0p	161	98	negative	negative	JJ
cord-265978-i0fu8e0p	161	99	controls	control	NNS
cord-265978-i0fu8e0p	161	100	(	(	-LRB-
cord-265978-i0fu8e0p	161	101	water	water	NN
cord-265978-i0fu8e0p	161	102	,	,	,
cord-265978-i0fu8e0p	161	103	nasal	nasal	NN
cord-265978-i0fu8e0p	161	104	/	/	SYM
cord-265978-i0fu8e0p	161	105	fecal	fecal	JJ
cord-265978-i0fu8e0p	161	106	suspensions	suspension	NNS
cord-265978-i0fu8e0p	161	107	and	and	CC
cord-265978-i0fu8e0p	161	108	some	some	DT
cord-265978-i0fu8e0p	161	109	irrelevant	irrelevant	JJ
cord-265978-i0fu8e0p	161	110	human	human	NN
cord-265978-i0fu8e0p	161	111	and	and	CC
cord-265978-i0fu8e0p	161	112	poultry	poultry	NN
cord-265978-i0fu8e0p	161	113	viruses	virus	NNS
cord-265978-i0fu8e0p	161	114	)	)	-RRB-
cord-265978-i0fu8e0p	161	115	.	.	.
cord-265978-i0fu8e0p	162	1	Although	although	IN
cord-265978-i0fu8e0p	162	2	different	different	JJ
cord-265978-i0fu8e0p	162	3	types	type	NNS
cord-265978-i0fu8e0p	162	4	of	of	IN
cord-265978-i0fu8e0p	162	5	group	group	NN
cord-265978-i0fu8e0p	162	6	II	II	NNP
cord-265978-i0fu8e0p	162	7	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	162	8	,	,	,
cord-265978-i0fu8e0p	162	9	such	such	JJ
cord-265978-i0fu8e0p	162	10	as	as	IN
cord-265978-i0fu8e0p	162	11	HCV	HCV	NNP
cord-265978-i0fu8e0p	162	12	-	-	HYPH
cord-265978-i0fu8e0p	162	13	OC43	OC43	NNP
cord-265978-i0fu8e0p	162	14	,	,	,
cord-265978-i0fu8e0p	162	15	PHEV	PHEV	NNP
cord-265978-i0fu8e0p	162	16	,	,	,
cord-265978-i0fu8e0p	162	17	CRCoV	crcov	NN
cord-265978-i0fu8e0p	162	18	,	,	,
cord-265978-i0fu8e0p	162	19	HECoV	hecov	CD
cord-265978-i0fu8e0p	162	20	,	,	,
cord-265978-i0fu8e0p	162	21	were	be	VBD
cord-265978-i0fu8e0p	162	22	not	not	RB
cord-265978-i0fu8e0p	162	23	included	include	VBN
cord-265978-i0fu8e0p	162	24	in	in	IN
cord-265978-i0fu8e0p	162	25	the	the	DT
cord-265978-i0fu8e0p	162	26	specificity	specificity	NN
cord-265978-i0fu8e0p	162	27	evaluation	evaluation	NN
cord-265978-i0fu8e0p	162	28	as	as	IN
cord-265978-i0fu8e0p	162	29	a	a	DT
cord-265978-i0fu8e0p	162	30	result	result	NN
cord-265978-i0fu8e0p	162	31	of	of	IN
cord-265978-i0fu8e0p	162	32	their	-PRON-	PRP$
cord-265978-i0fu8e0p	162	33	unavailability	unavailability	NN
cord-265978-i0fu8e0p	162	34	during	during	IN
cord-265978-i0fu8e0p	162	35	the	the	DT
cord-265978-i0fu8e0p	162	36	assay	assay	NN
cord-265978-i0fu8e0p	162	37	development	development	NN
cord-265978-i0fu8e0p	162	38	,	,	,
cord-265978-i0fu8e0p	162	39	it	-PRON-	PRP
cord-265978-i0fu8e0p	162	40	is	be	VBZ
cord-265978-i0fu8e0p	162	41	highly	highly	RB
cord-265978-i0fu8e0p	162	42	expected	expect	VBN
cord-265978-i0fu8e0p	162	43	that	that	IN
cord-265978-i0fu8e0p	162	44	these	these	DT
cord-265978-i0fu8e0p	162	45	viruses	virus	NNS
cord-265978-i0fu8e0p	162	46	can	can	MD
cord-265978-i0fu8e0p	162	47	generate	generate	VB
cord-265978-i0fu8e0p	162	48	positive	positive	JJ
cord-265978-i0fu8e0p	162	49	signals	signal	NNS
cord-265978-i0fu8e0p	162	50	depending	depend	VBG
cord-265978-i0fu8e0p	162	51	on	on	IN
cord-265978-i0fu8e0p	162	52	their	-PRON-	PRP$
cord-265978-i0fu8e0p	162	53	sequence	sequence	NN
cord-265978-i0fu8e0p	162	54	homology	homology	NN
cord-265978-i0fu8e0p	162	55	with	with	IN
cord-265978-i0fu8e0p	162	56	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	162	57	target	target	NN
cord-265978-i0fu8e0p	162	58	region	region	NN
cord-265978-i0fu8e0p	162	59	.	.	.
cord-265978-i0fu8e0p	163	1	The	the	DT
cord-265978-i0fu8e0p	163	2	detection	detection	NN
cord-265978-i0fu8e0p	163	3	limit	limit	NN
cord-265978-i0fu8e0p	163	4	of	of	IN
cord-265978-i0fu8e0p	163	5	the	the	DT
cord-265978-i0fu8e0p	163	6	assay	assay	NN
cord-265978-i0fu8e0p	163	7	was	be	VBD
cord-265978-i0fu8e0p	163	8	evaluated	evaluate	VBN
cord-265978-i0fu8e0p	163	9	by	by	IN
cord-265978-i0fu8e0p	163	10	both	both	DT
cord-265978-i0fu8e0p	163	11	cDNA	cdna	NN
cord-265978-i0fu8e0p	163	12	copy	copy	NN
cord-265978-i0fu8e0p	163	13	numbers	number	NNS
cord-265978-i0fu8e0p	163	14	and	and	CC
cord-265978-i0fu8e0p	163	15	TCID	TCID	NNP
cord-265978-i0fu8e0p	163	16	50	50	CD
cord-265978-i0fu8e0p	163	17	.	.	.
cord-265978-i0fu8e0p	164	1	Ten	ten	CD
cord-265978-i0fu8e0p	164	2	-	-	HYPH
cord-265978-i0fu8e0p	164	3	fold	fold	JJ
cord-265978-i0fu8e0p	164	4	serial	serial	JJ
cord-265978-i0fu8e0p	164	5	dilutions	dilution	NNS
cord-265978-i0fu8e0p	164	6	of	of	IN
cord-265978-i0fu8e0p	164	7	the	the	DT
cord-265978-i0fu8e0p	164	8	DNA	dna	NN
cord-265978-i0fu8e0p	164	9	standard	standard	NN
cord-265978-i0fu8e0p	164	10	as	as	RB
cord-265978-i0fu8e0p	164	11	well	well	RB
cord-265978-i0fu8e0p	164	12	as	as	IN
cord-265978-i0fu8e0p	164	13	the	the	DT
cord-265978-i0fu8e0p	164	14	virus	virus	NN
cord-265978-i0fu8e0p	164	15	stock	stock	NN
cord-265978-i0fu8e0p	164	16	of	of	IN
cord-265978-i0fu8e0p	164	17	known	know	VBN
cord-265978-i0fu8e0p	164	18	titer	titer	NN
cord-265978-i0fu8e0p	164	19	(	(	-LRB-
cord-265978-i0fu8e0p	164	20	10	10	CD
cord-265978-i0fu8e0p	164	21	5	5	CD
cord-265978-i0fu8e0p	164	22	TCID	TCID	NNP
cord-265978-i0fu8e0p	164	23	50	50	CD
cord-265978-i0fu8e0p	165	1	/ml	/ml	NFP
cord-265978-i0fu8e0p	165	2	)	)	-RRB-
cord-265978-i0fu8e0p	165	3	were	be	VBD
cord-265978-i0fu8e0p	165	4	used	use	VBN
cord-265978-i0fu8e0p	165	5	to	to	TO
cord-265978-i0fu8e0p	165	6	determine	determine	VB
cord-265978-i0fu8e0p	165	7	the	the	DT
cord-265978-i0fu8e0p	165	8	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	165	9	and	and	CC
cord-265978-i0fu8e0p	165	10	linearity	linearity	NN
cord-265978-i0fu8e0p	165	11	of	of	IN
cord-265978-i0fu8e0p	165	12	the	the	DT
cord-265978-i0fu8e0p	165	13	assay	assay	NN
cord-265978-i0fu8e0p	165	14	as	as	RB
cord-265978-i0fu8e0p	165	15	well	well	RB
cord-265978-i0fu8e0p	165	16	as	as	IN
cord-265978-i0fu8e0p	165	17	to	to	TO
cord-265978-i0fu8e0p	165	18	generate	generate	VB
cord-265978-i0fu8e0p	165	19	standard	standard	JJ
cord-265978-i0fu8e0p	165	20	curves	curve	NNS
cord-265978-i0fu8e0p	165	21	applicable	applicable	JJ
cord-265978-i0fu8e0p	165	22	for	for	IN
cord-265978-i0fu8e0p	165	23	absolute	absolute	JJ
cord-265978-i0fu8e0p	165	24	quantification	quantification	NN
cord-265978-i0fu8e0p	165	25	of	of	IN
cord-265978-i0fu8e0p	165	26	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	165	27	in	in	IN
cord-265978-i0fu8e0p	165	28	clinical	clinical	JJ
cord-265978-i0fu8e0p	165	29	samples	sample	NNS
cord-265978-i0fu8e0p	165	30	.	.	.
cord-265978-i0fu8e0p	166	1	The	the	DT
cord-265978-i0fu8e0p	166	2	assay	assay	NN
cord-265978-i0fu8e0p	166	3	was	be	VBD
cord-265978-i0fu8e0p	166	4	able	able	JJ
cord-265978-i0fu8e0p	166	5	to	to	TO
cord-265978-i0fu8e0p	166	6	detect	detect	VB
cord-265978-i0fu8e0p	166	7	10-fold	10-fold	JJ
cord-265978-i0fu8e0p	166	8	differences	difference	NNS
cord-265978-i0fu8e0p	166	9	of	of	IN
cord-265978-i0fu8e0p	166	10	both	both	DT
cord-265978-i0fu8e0p	166	11	targets	target	NNS
cord-265978-i0fu8e0p	166	12	over	over	IN
cord-265978-i0fu8e0p	166	13	6e8	6e8	CD
cord-265978-i0fu8e0p	166	14	orders	order	NNS
cord-265978-i0fu8e0p	166	15	of	of	IN
cord-265978-i0fu8e0p	166	16	magnitude	magnitude	NN
cord-265978-i0fu8e0p	166	17	.	.	.
cord-265978-i0fu8e0p	167	1	A	a	DT
cord-265978-i0fu8e0p	167	2	minimum	minimum	NN
cord-265978-i0fu8e0p	167	3	of	of	IN
cord-265978-i0fu8e0p	167	4	10	10	CD
cord-265978-i0fu8e0p	167	5	3	3	CD
cord-265978-i0fu8e0p	167	6	cDNA	cdna	NN
cord-265978-i0fu8e0p	167	7	copies	copy	NNS
cord-265978-i0fu8e0p	167	8	of	of	IN
cord-265978-i0fu8e0p	167	9	the	the	DT
cord-265978-i0fu8e0p	167	10	plasmid	plasmid	NN
cord-265978-i0fu8e0p	167	11	standard	standard	NN
cord-265978-i0fu8e0p	167	12	and	and	CC
cord-265978-i0fu8e0p	167	13	1.17	1.17	CD
cord-265978-i0fu8e0p	167	14	Â	â	CD
cord-265978-i0fu8e0p	167	15	10	10	CD
cord-265978-i0fu8e0p	167	16	À3	à3	CD
cord-265978-i0fu8e0p	167	17	TCID	TCID	NNP
cord-265978-i0fu8e0p	167	18	50	50	CD
cord-265978-i0fu8e0p	167	19	of	of	IN
cord-265978-i0fu8e0p	167	20	the	the	DT
cord-265978-i0fu8e0p	167	21	cell	cell	NN
cord-265978-i0fu8e0p	167	22	-	-	HYPH
cord-265978-i0fu8e0p	167	23	culture	culture	NN
cord-265978-i0fu8e0p	167	24	grown	grow	VBN
cord-265978-i0fu8e0p	167	25	virus	virus	NN
cord-265978-i0fu8e0p	167	26	per	per	IN
cord-265978-i0fu8e0p	167	27	reaction	reaction	NN
cord-265978-i0fu8e0p	167	28	was	be	VBD
cord-265978-i0fu8e0p	167	29	recognized	recognize	VBN
cord-265978-i0fu8e0p	167	30	(	(	-LRB-
cord-265978-i0fu8e0p	167	31	Fig	Fig	NNP
cord-265978-i0fu8e0p	167	32	.	.	.
cord-265978-i0fu8e0p	167	33	1	1	CD
cord-265978-i0fu8e0p	167	34	)	)	-RRB-
cord-265978-i0fu8e0p	167	35	.	.	.
cord-265978-i0fu8e0p	168	1	In	in	IN
cord-265978-i0fu8e0p	168	2	addition	addition	NN
cord-265978-i0fu8e0p	168	3	,	,	,
cord-265978-i0fu8e0p	168	4	the	the	DT
cord-265978-i0fu8e0p	168	5	assay	assay	NN
cord-265978-i0fu8e0p	168	6	showed	show	VBD
cord-265978-i0fu8e0p	168	7	to	to	TO
cord-265978-i0fu8e0p	168	8	be	be	VB
cord-265978-i0fu8e0p	168	9	highly	highly	RB
cord-265978-i0fu8e0p	168	10	reproducible	reproducible	JJ
cord-265978-i0fu8e0p	168	11	with	with	IN
cord-265978-i0fu8e0p	168	12	very	very	RB
cord-265978-i0fu8e0p	168	13	limited	limited	JJ
cord-265978-i0fu8e0p	168	14	intra	intra	NN
cord-265978-i0fu8e0p	168	15	-	-	:
cord-265978-i0fu8e0p	168	16	and	and	CC
cord-265978-i0fu8e0p	168	17	inter	inter	JJ
cord-265978-i0fu8e0p	168	18	-	-	JJ
cord-265978-i0fu8e0p	168	19	assay	assay	JJ
cord-265978-i0fu8e0p	168	20	variations	variation	NNS
cord-265978-i0fu8e0p	168	21	.	.	.
cord-265978-i0fu8e0p	169	1	The	the	DT
cord-265978-i0fu8e0p	169	2	calculated	calculate	VBN
cord-265978-i0fu8e0p	169	3	CV	cv	NN
cord-265978-i0fu8e0p	169	4	values	value	NNS
cord-265978-i0fu8e0p	169	5	for	for	IN
cord-265978-i0fu8e0p	169	6	both	both	DT
cord-265978-i0fu8e0p	169	7	parameters	parameter	NNS
cord-265978-i0fu8e0p	169	8	did	do	VBD
cord-265978-i0fu8e0p	169	9	not	not	RB
cord-265978-i0fu8e0p	169	10	rise	rise	VB
cord-265978-i0fu8e0p	169	11	over	over	IN
cord-265978-i0fu8e0p	169	12	2.5	2.5	CD
cord-265978-i0fu8e0p	169	13	%	%	NN
cord-265978-i0fu8e0p	169	14	.	.	.
cord-265978-i0fu8e0p	170	1	A	a	DT
cord-265978-i0fu8e0p	170	2	mean	mean	JJ
cord-265978-i0fu8e0p	170	3	C.V	C.V	NNP
cord-265978-i0fu8e0p	170	4	that	that	WDT
cord-265978-i0fu8e0p	170	5	is	be	VBZ
cord-265978-i0fu8e0p	170	6	lower	low	JJR
cord-265978-i0fu8e0p	170	7	than	than	IN
cord-265978-i0fu8e0p	170	8	5	5	CD
cord-265978-i0fu8e0p	170	9	%	%	NN
cord-265978-i0fu8e0p	170	10	is	be	VBZ
cord-265978-i0fu8e0p	170	11	acceptable	acceptable	JJ
cord-265978-i0fu8e0p	170	12	and	and	CC
cord-265978-i0fu8e0p	170	13	indicates	indicate	VBZ
cord-265978-i0fu8e0p	170	14	that	that	IN
cord-265978-i0fu8e0p	170	15	the	the	DT
cord-265978-i0fu8e0p	170	16	assay	assay	NN
cord-265978-i0fu8e0p	170	17	can	can	MD
cord-265978-i0fu8e0p	170	18	generate	generate	VB
cord-265978-i0fu8e0p	170	19	reproducible	reproducible	JJ
cord-265978-i0fu8e0p	170	20	results	result	NNS
cord-265978-i0fu8e0p	170	21	[	[	-LRB-
cord-265978-i0fu8e0p	170	22	28	28	CD
cord-265978-i0fu8e0p	170	23	]	]	-RRB-
cord-265978-i0fu8e0p	170	24	.	.	.
cord-265978-i0fu8e0p	171	1	The	the	DT
cord-265978-i0fu8e0p	171	2	diagnostic	diagnostic	JJ
cord-265978-i0fu8e0p	171	3	applicability	applicability	NN
cord-265978-i0fu8e0p	171	4	of	of	IN
cord-265978-i0fu8e0p	171	5	the	the	DT
cord-265978-i0fu8e0p	171	6	developed	developed	JJ
cord-265978-i0fu8e0p	171	7	assay	assay	NN
cord-265978-i0fu8e0p	171	8	was	be	VBD
cord-265978-i0fu8e0p	171	9	established	establish	VBN
cord-265978-i0fu8e0p	171	10	by	by	IN
cord-265978-i0fu8e0p	171	11	testing	test	VBG
cord-265978-i0fu8e0p	171	12	this	this	DT
cord-265978-i0fu8e0p	171	13	method	method	NN
cord-265978-i0fu8e0p	171	14	on	on	IN
cord-265978-i0fu8e0p	171	15	clinical	clinical	JJ
cord-265978-i0fu8e0p	171	16	samples	sample	NNS
cord-265978-i0fu8e0p	171	17	and	and	CC
cord-265978-i0fu8e0p	171	18	comparing	compare	VBG
cord-265978-i0fu8e0p	171	19	its	-PRON-	PRP$
cord-265978-i0fu8e0p	171	20	results	result	NNS
cord-265978-i0fu8e0p	171	21	with	with	IN
cord-265978-i0fu8e0p	171	22	the	the	DT
cord-265978-i0fu8e0p	171	23	conventional	conventional	JJ
cord-265978-i0fu8e0p	171	24	RT	RT	NNP
cord-265978-i0fu8e0p	171	25	-	-	HYPH
cord-265978-i0fu8e0p	171	26	PCR	PCR	NNP
cord-265978-i0fu8e0p	171	27	assay	assay	NN
cord-265978-i0fu8e0p	171	28	.	.	.
cord-265978-i0fu8e0p	172	1	Out	out	IN
cord-265978-i0fu8e0p	172	2	of	of	IN
cord-265978-i0fu8e0p	172	3	34	34	CD
cord-265978-i0fu8e0p	172	4	positive	positive	JJ
cord-265978-i0fu8e0p	172	5	samples	sample	NNS
cord-265978-i0fu8e0p	172	6	by	by	IN
cord-265978-i0fu8e0p	172	7	real	real	JJ
cord-265978-i0fu8e0p	172	8	-	-	HYPH
cord-265978-i0fu8e0p	172	9	time	time	NN
cord-265978-i0fu8e0p	172	10	RT	RT	NNP
cord-265978-i0fu8e0p	172	11	-	-	HYPH
cord-265978-i0fu8e0p	172	12	PCR	PCR	NNP
cord-265978-i0fu8e0p	172	13	(	(	-LRB-
cord-265978-i0fu8e0p	172	14	20	20	CD
cord-265978-i0fu8e0p	172	15	fecal	fecal	JJ
cord-265978-i0fu8e0p	172	16	and	and	CC
cord-265978-i0fu8e0p	172	17	14	14	CD
cord-265978-i0fu8e0p	172	18	nasal	nasal	NN
cord-265978-i0fu8e0p	172	19	)	)	-RRB-
cord-265978-i0fu8e0p	172	20	,	,	,
cord-265978-i0fu8e0p	172	21	five	five	CD
cord-265978-i0fu8e0p	172	22	samples	sample	NNS
cord-265978-i0fu8e0p	172	23	(	(	-LRB-
cord-265978-i0fu8e0p	172	24	three	three	CD
cord-265978-i0fu8e0p	172	25	fecal	fecal	JJ
cord-265978-i0fu8e0p	172	26	and	and	CC
cord-265978-i0fu8e0p	172	27	two	two	CD
cord-265978-i0fu8e0p	172	28	nasal	nasal	NN
cord-265978-i0fu8e0p	172	29	)	)	-RRB-
cord-265978-i0fu8e0p	172	30	were	be	VBD
cord-265978-i0fu8e0p	172	31	diagnosed	diagnose	VBN
cord-265978-i0fu8e0p	172	32	negative	negative	JJ
cord-265978-i0fu8e0p	172	33	by	by	IN
cord-265978-i0fu8e0p	172	34	the	the	DT
cord-265978-i0fu8e0p	172	35	gelbased	gelbased	JJ
cord-265978-i0fu8e0p	172	36	assay	assay	NN
cord-265978-i0fu8e0p	172	37	.	.	.
cord-265978-i0fu8e0p	173	1	As	as	IN
cord-265978-i0fu8e0p	173	2	expected	expect	VBN
cord-265978-i0fu8e0p	173	3	,	,	,
cord-265978-i0fu8e0p	173	4	these	these	DT
cord-265978-i0fu8e0p	173	5	samples	sample	NNS
cord-265978-i0fu8e0p	173	6	contained	contain	VBD
cord-265978-i0fu8e0p	173	7	the	the	DT
cord-265978-i0fu8e0p	173	8	least	least	JJS
cord-265978-i0fu8e0p	173	9	virus	virus	NN
cord-265978-i0fu8e0p	173	10	copy	copy	NN
cord-265978-i0fu8e0p	173	11	number	number	NN
cord-265978-i0fu8e0p	173	12	among	among	IN
cord-265978-i0fu8e0p	173	13	all	all	DT
cord-265978-i0fu8e0p	173	14	positive	positive	JJ
cord-265978-i0fu8e0p	173	15	samples	sample	NNS
cord-265978-i0fu8e0p	173	16	.	.	.
cord-265978-i0fu8e0p	174	1	On	on	IN
cord-265978-i0fu8e0p	174	2	the	the	DT
cord-265978-i0fu8e0p	174	3	other	other	JJ
cord-265978-i0fu8e0p	174	4	hand	hand	NN
cord-265978-i0fu8e0p	174	5	,	,	,
cord-265978-i0fu8e0p	174	6	all	all	PDT
cord-265978-i0fu8e0p	174	7	the	the	DT
cord-265978-i0fu8e0p	174	8	negative	negative	JJ
cord-265978-i0fu8e0p	174	9	samples	sample	NNS
cord-265978-i0fu8e0p	174	10	identified	identify	VBN
cord-265978-i0fu8e0p	174	11	by	by	IN
cord-265978-i0fu8e0p	174	12	the	the	DT
cord-265978-i0fu8e0p	174	13	real	real	JJ
cord-265978-i0fu8e0p	174	14	-	-	HYPH
cord-265978-i0fu8e0p	174	15	time	time	NN
cord-265978-i0fu8e0p	174	16	assay	assay	NN
cord-265978-i0fu8e0p	174	17	were	be	VBD
cord-265978-i0fu8e0p	174	18	also	also	RB
cord-265978-i0fu8e0p	174	19	negative	negative	JJ
cord-265978-i0fu8e0p	174	20	by	by	IN
cord-265978-i0fu8e0p	174	21	the	the	DT
cord-265978-i0fu8e0p	174	22	conventional	conventional	JJ
cord-265978-i0fu8e0p	174	23	method	method	NN
cord-265978-i0fu8e0p	174	24	.	.	.
cord-265978-i0fu8e0p	175	1	This	this	DT
cord-265978-i0fu8e0p	175	2	confirms	confirm	VBZ
cord-265978-i0fu8e0p	175	3	the	the	DT
cord-265978-i0fu8e0p	175	4	higher	high	JJR
cord-265978-i0fu8e0p	175	5	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	175	6	of	of	IN
cord-265978-i0fu8e0p	175	7	the	the	DT
cord-265978-i0fu8e0p	175	8	developed	developed	JJ
cord-265978-i0fu8e0p	175	9	assay	assay	NN
cord-265978-i0fu8e0p	175	10	and	and	CC
cord-265978-i0fu8e0p	175	11	its	-PRON-	PRP$
cord-265978-i0fu8e0p	175	12	reliability	reliability	NN
cord-265978-i0fu8e0p	175	13	for	for	IN
cord-265978-i0fu8e0p	175	14	generation	generation	NN
cord-265978-i0fu8e0p	175	15	of	of	IN
cord-265978-i0fu8e0p	175	16	an	an	DT
cord-265978-i0fu8e0p	175	17	appropriate	appropriate	JJ
cord-265978-i0fu8e0p	175	18	epidemiological	epidemiological	JJ
cord-265978-i0fu8e0p	175	19	data	datum	NNS
cord-265978-i0fu8e0p	175	20	.	.	.
cord-265978-i0fu8e0p	176	1	Moreover	moreover	RB
cord-265978-i0fu8e0p	176	2	,	,	,
cord-265978-i0fu8e0p	176	3	the	the	DT
cord-265978-i0fu8e0p	176	4	assay	assay	NN
cord-265978-i0fu8e0p	176	5	proved	prove	VBD
cord-265978-i0fu8e0p	176	6	its	-PRON-	PRP$
cord-265978-i0fu8e0p	176	7	ability	ability	NN
cord-265978-i0fu8e0p	176	8	to	to	TO
cord-265978-i0fu8e0p	176	9	detect	detect	VB
cord-265978-i0fu8e0p	176	10	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	176	11	over	over	IN
cord-265978-i0fu8e0p	176	12	a	a	DT
cord-265978-i0fu8e0p	176	13	wide	wide	JJ
cord-265978-i0fu8e0p	176	14	dynamic	dynamic	JJ
cord-265978-i0fu8e0p	176	15	range	range	NN
cord-265978-i0fu8e0p	176	16	(	(	-LRB-
cord-265978-i0fu8e0p	176	17	5.5	5.5	CD
cord-265978-i0fu8e0p	176	18	logs	log	NNS
cord-265978-i0fu8e0p	176	19	of	of	IN
cord-265978-i0fu8e0p	176	20	magnitude	magnitude	NN
cord-265978-i0fu8e0p	176	21	for	for	IN
cord-265978-i0fu8e0p	176	22	nasal	nasal	NN
cord-265978-i0fu8e0p	176	23	samples	sample	NNS
cord-265978-i0fu8e0p	176	24	and	and	CC
cord-265978-i0fu8e0p	176	25	4	4	CD
cord-265978-i0fu8e0p	176	26	logs	log	NNS
cord-265978-i0fu8e0p	176	27	for	for	IN
cord-265978-i0fu8e0p	176	28	fecal	fecal	JJ
cord-265978-i0fu8e0p	176	29	samples	sample	NNS
cord-265978-i0fu8e0p	176	30	)	)	-RRB-
cord-265978-i0fu8e0p	176	31	.	.	.
cord-265978-i0fu8e0p	177	1	The	the	DT
cord-265978-i0fu8e0p	177	2	limited	limited	JJ
cord-265978-i0fu8e0p	177	3	dynamic	dynamic	JJ
cord-265978-i0fu8e0p	177	4	range	range	NN
cord-265978-i0fu8e0p	177	5	of	of	IN
cord-265978-i0fu8e0p	177	6	fecal	fecal	JJ
cord-265978-i0fu8e0p	177	7	samples	sample	NNS
cord-265978-i0fu8e0p	177	8	as	as	IN
cord-265978-i0fu8e0p	177	9	compared	compare	VBN
cord-265978-i0fu8e0p	177	10	to	to	IN
cord-265978-i0fu8e0p	177	11	the	the	DT
cord-265978-i0fu8e0p	177	12	nasal	nasal	NN
cord-265978-i0fu8e0p	177	13	samples	sample	NNS
cord-265978-i0fu8e0p	177	14	may	may	MD
cord-265978-i0fu8e0p	177	15	be	be	VB
cord-265978-i0fu8e0p	177	16	explained	explain	VBN
cord-265978-i0fu8e0p	177	17	by	by	IN
cord-265978-i0fu8e0p	177	18	the	the	DT
cord-265978-i0fu8e0p	177	19	higher	high	JJR
cord-265978-i0fu8e0p	177	20	background	background	NN
cord-265978-i0fu8e0p	177	21	of	of	IN
cord-265978-i0fu8e0p	177	22	inhibitory	inhibitory	JJ
cord-265978-i0fu8e0p	177	23	substances	substance	NNS
cord-265978-i0fu8e0p	177	24	in	in	IN
cord-265978-i0fu8e0p	177	25	fecal	fecal	JJ
cord-265978-i0fu8e0p	177	26	material	material	NN
cord-265978-i0fu8e0p	177	27	.	.	.
cord-265978-i0fu8e0p	178	1	Recently	recently	RB
cord-265978-i0fu8e0p	178	2	,	,	,
cord-265978-i0fu8e0p	178	3	three	three	CD
cord-265978-i0fu8e0p	178	4	reports	report	NNS
cord-265978-i0fu8e0p	178	5	described	describe	VBD
cord-265978-i0fu8e0p	178	6	the	the	DT
cord-265978-i0fu8e0p	178	7	process	process	NN
cord-265978-i0fu8e0p	178	8	of	of	IN
cord-265978-i0fu8e0p	178	9	establishment	establishment	NN
cord-265978-i0fu8e0p	178	10	of	of	IN
cord-265978-i0fu8e0p	178	11	real	real	JJ
cord-265978-i0fu8e0p	178	12	-	-	HYPH
cord-265978-i0fu8e0p	178	13	time	time	NN
cord-265978-i0fu8e0p	178	14	RT	rt	NN
cord-265978-i0fu8e0p	178	15	-	-	HYPH
cord-265978-i0fu8e0p	178	16	PCR	PCR	NNP
cord-265978-i0fu8e0p	178	17	assays	assay	NNS
cord-265978-i0fu8e0p	178	18	for	for	IN
cord-265978-i0fu8e0p	178	19	detection	detection	NN
cord-265978-i0fu8e0p	178	20	of	of	IN
cord-265978-i0fu8e0p	178	21	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	178	22	either	either	CC
cord-265978-i0fu8e0p	178	23	solely	solely	RB
cord-265978-i0fu8e0p	178	24	or	or	CC
cord-265978-i0fu8e0p	178	25	in	in	IN
cord-265978-i0fu8e0p	178	26	a	a	DT
cord-265978-i0fu8e0p	178	27	multiplex	multiplex	NN
cord-265978-i0fu8e0p	178	28	set	set	NN
cord-265978-i0fu8e0p	178	29	[	[	-LRB-
cord-265978-i0fu8e0p	178	30	13	13	CD
cord-265978-i0fu8e0p	178	31	,	,	,
cord-265978-i0fu8e0p	178	32	29	29	CD
cord-265978-i0fu8e0p	178	33	,	,	,
cord-265978-i0fu8e0p	178	34	30	30	CD
cord-265978-i0fu8e0p	178	35	]	]	-RRB-
cord-265978-i0fu8e0p	178	36	.	.	.
cord-265978-i0fu8e0p	179	1	However	however	RB
cord-265978-i0fu8e0p	179	2	,	,	,
cord-265978-i0fu8e0p	179	3	none	none	NN
cord-265978-i0fu8e0p	179	4	of	of	IN
cord-265978-i0fu8e0p	179	5	these	these	DT
cord-265978-i0fu8e0p	179	6	systems	system	NNS
cord-265978-i0fu8e0p	179	7	utilized	utilize	VBD
cord-265978-i0fu8e0p	179	8	the	the	DT
cord-265978-i0fu8e0p	179	9	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	179	10	Green	Green	NNP
cord-265978-i0fu8e0p	179	11	I	-PRON-	PRP
cord-265978-i0fu8e0p	179	12	chemistry	chemistry	VBP
cord-265978-i0fu8e0p	179	13	for	for	IN
cord-265978-i0fu8e0p	179	14	specific	specific	JJ
cord-265978-i0fu8e0p	179	15	detection	detection	NN
cord-265978-i0fu8e0p	179	16	and	and	CC
cord-265978-i0fu8e0p	179	17	quantification	quantification	NN
cord-265978-i0fu8e0p	179	18	of	of	IN
cord-265978-i0fu8e0p	179	19	BCoV.	bcov.	CD
cord-265978-i0fu8e0p	180	1	The	the	DT
cord-265978-i0fu8e0p	180	2	main	main	JJ
cord-265978-i0fu8e0p	180	3	advantages	advantage	NNS
cord-265978-i0fu8e0p	180	4	of	of	IN
cord-265978-i0fu8e0p	180	5	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	180	6	Green	Green	NNP
cord-265978-i0fu8e0p	180	7	I	-PRON-	PRP
cord-265978-i0fu8e0p	180	8	over	over	IN
cord-265978-i0fu8e0p	180	9	other	other	JJ
cord-265978-i0fu8e0p	180	10	real	real	JJ
cord-265978-i0fu8e0p	180	11	-	-	HYPH
cord-265978-i0fu8e0p	180	12	time	time	NN
cord-265978-i0fu8e0p	180	13	PCR	PCR	NNP
cord-265978-i0fu8e0p	180	14	detection	detection	NN
cord-265978-i0fu8e0p	180	15	formats	format	NNS
cord-265978-i0fu8e0p	180	16	are	be	VBP
cord-265978-i0fu8e0p	180	17	;	;	:
cord-265978-i0fu8e0p	180	18	a	a	LS
cord-265978-i0fu8e0p	180	19	)	)	-RRB-
cord-265978-i0fu8e0p	180	20	it	-PRON-	PRP
cord-265978-i0fu8e0p	180	21	is	be	VBZ
cord-265978-i0fu8e0p	180	22	a	a	DT
cord-265978-i0fu8e0p	180	23	low	low	JJ
cord-265978-i0fu8e0p	180	24	-	-	HYPH
cord-265978-i0fu8e0p	180	25	cost	cost	NN
cord-265978-i0fu8e0p	180	26	fluorochrome	fluorochrome	NN
cord-265978-i0fu8e0p	180	27	,	,	,
cord-265978-i0fu8e0p	181	1	b	b	LS
cord-265978-i0fu8e0p	181	2	)	)	-RRB-
cord-265978-i0fu8e0p	181	3	it	-PRON-	PRP
cord-265978-i0fu8e0p	181	4	is	be	VBZ
cord-265978-i0fu8e0p	181	5	a	a	DT
cord-265978-i0fu8e0p	181	6	simpler	simple	JJR
cord-265978-i0fu8e0p	181	7	approach	approach	NN
cord-265978-i0fu8e0p	181	8	especially	especially	RB
cord-265978-i0fu8e0p	181	9	for	for	IN
cord-265978-i0fu8e0p	181	10	primer	primer	NN
cord-265978-i0fu8e0p	181	11	design	design	NN
cord-265978-i0fu8e0p	181	12	and	and	CC
cord-265978-i0fu8e0p	181	13	optimization	optimization	NN
cord-265978-i0fu8e0p	181	14	procedures	procedure	NNS
cord-265978-i0fu8e0p	181	15	[	[	-LRB-
cord-265978-i0fu8e0p	181	16	31	31	CD
cord-265978-i0fu8e0p	181	17	]	]	-RRB-
cord-265978-i0fu8e0p	181	18	,	,	,
cord-265978-i0fu8e0p	181	19	and	and	CC
cord-265978-i0fu8e0p	181	20	c	c	LS
cord-265978-i0fu8e0p	181	21	)	)	-RRB-
cord-265978-i0fu8e0p	181	22	the	the	DT
cord-265978-i0fu8e0p	181	23	artifacts	artifact	NNS
cord-265978-i0fu8e0p	181	24	commonly	commonly	RB
cord-265978-i0fu8e0p	181	25	observed	observe	VBD
cord-265978-i0fu8e0p	181	26	in	in	IN
cord-265978-i0fu8e0p	181	27	specific	specific	JJ
cord-265978-i0fu8e0p	181	28	probes	probe	NNS
cord-265978-i0fu8e0p	181	29	,	,	,
cord-265978-i0fu8e0p	181	30	particularly	particularly	RB
cord-265978-i0fu8e0p	181	31	at	at	IN
cord-265978-i0fu8e0p	181	32	amplification	amplification	NN
cord-265978-i0fu8e0p	181	33	cycles	cycle	NNS
cord-265978-i0fu8e0p	181	34	beyond	beyond	IN
cord-265978-i0fu8e0p	181	35	the	the	DT
cord-265978-i0fu8e0p	181	36	30th	30th	NN
cord-265978-i0fu8e0p	181	37	,	,	,
cord-265978-i0fu8e0p	181	38	are	be	VBP
cord-265978-i0fu8e0p	181	39	minimal	minimal	JJ
cord-265978-i0fu8e0p	181	40	and	and	CC
cord-265978-i0fu8e0p	181	41	can	can	MD
cord-265978-i0fu8e0p	181	42	be	be	VB
cord-265978-i0fu8e0p	181	43	ruled	rule	VBN
cord-265978-i0fu8e0p	181	44	out	out	RP
cord-265978-i0fu8e0p	181	45	by	by	IN
cord-265978-i0fu8e0p	181	46	melt	melt	NN
cord-265978-i0fu8e0p	181	47	curve	curve	NN
cord-265978-i0fu8e0p	181	48	analysis	analysis	NN
cord-265978-i0fu8e0p	181	49	[	[	-LRB-
cord-265978-i0fu8e0p	181	50	32	32	CD
cord-265978-i0fu8e0p	181	51	]	]	-RRB-
cord-265978-i0fu8e0p	181	52	.	.	.
cord-265978-i0fu8e0p	182	1	In	in	IN
cord-265978-i0fu8e0p	182	2	conclusion	conclusion	NN
cord-265978-i0fu8e0p	182	3	,	,	,
cord-265978-i0fu8e0p	182	4	the	the	DT
cord-265978-i0fu8e0p	182	5	real	real	JJ
cord-265978-i0fu8e0p	182	6	-	-	HYPH
cord-265978-i0fu8e0p	182	7	time	time	NN
cord-265978-i0fu8e0p	182	8	PCR	PCR	NNP
cord-265978-i0fu8e0p	182	9	described	describe	VBN
cord-265978-i0fu8e0p	182	10	here	here	RB
cord-265978-i0fu8e0p	182	11	is	be	VBZ
cord-265978-i0fu8e0p	182	12	a	a	DT
cord-265978-i0fu8e0p	182	13	sensitive	sensitive	JJ
cord-265978-i0fu8e0p	182	14	,	,	,
cord-265978-i0fu8e0p	182	15	simple	simple	JJ
cord-265978-i0fu8e0p	182	16	and	and	CC
cord-265978-i0fu8e0p	182	17	cost	cost	NN
cord-265978-i0fu8e0p	182	18	-	-	HYPH
cord-265978-i0fu8e0p	182	19	effective	effective	JJ
cord-265978-i0fu8e0p	182	20	method	method	NN
cord-265978-i0fu8e0p	182	21	that	that	WDT
cord-265978-i0fu8e0p	182	22	can	can	MD
cord-265978-i0fu8e0p	182	23	be	be	VB
cord-265978-i0fu8e0p	182	24	applied	apply	VBN
cord-265978-i0fu8e0p	182	25	easily	easily	RB
cord-265978-i0fu8e0p	182	26	for	for	IN
cord-265978-i0fu8e0p	182	27	laboratory	laboratory	NN
cord-265978-i0fu8e0p	182	28	diagnosis	diagnosis	NN
cord-265978-i0fu8e0p	182	29	of	of	IN
cord-265978-i0fu8e0p	182	30	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	182	31	infections	infection	NNS
cord-265978-i0fu8e0p	182	32	.	.	.
cord-265978-i0fu8e0p	183	1	The	the	DT
cord-265978-i0fu8e0p	183	2	method	method	NN
cord-265978-i0fu8e0p	183	3	is	be	VBZ
cord-265978-i0fu8e0p	183	4	convenient	convenient	JJ
cord-265978-i0fu8e0p	183	5	for	for	IN
cord-265978-i0fu8e0p	183	6	early	early	JJ
cord-265978-i0fu8e0p	183	7	differential	differential	JJ
cord-265978-i0fu8e0p	183	8	diagnosis	diagnosis	NN
cord-265978-i0fu8e0p	183	9	of	of	IN
cord-265978-i0fu8e0p	183	10	calf	calf	NN
cord-265978-i0fu8e0p	183	11	diarrhea	diarrhea	NN
cord-265978-i0fu8e0p	183	12	and	and	CC
cord-265978-i0fu8e0p	183	13	bovine	bovine	JJ
cord-265978-i0fu8e0p	183	14	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	183	15	disease	disease	NN
cord-265978-i0fu8e0p	183	16	syndromes	syndrome	NNS
cord-265978-i0fu8e0p	183	17	.	.	.
cord-265978-i0fu8e0p	184	1	In	in	IN
cord-265978-i0fu8e0p	184	2	addition	addition	NN
cord-265978-i0fu8e0p	184	3	,	,	,
cord-265978-i0fu8e0p	184	4	the	the	DT
cord-265978-i0fu8e0p	184	5	rapid	rapid	JJ
cord-265978-i0fu8e0p	184	6	results	result	NNS
cord-265978-i0fu8e0p	184	7	obtained	obtain	VBN
cord-265978-i0fu8e0p	184	8	by	by	IN
cord-265978-i0fu8e0p	184	9	this	this	DT
cord-265978-i0fu8e0p	184	10	method	method	NN
cord-265978-i0fu8e0p	184	11	are	be	VBP
cord-265978-i0fu8e0p	184	12	crucial	crucial	JJ
cord-265978-i0fu8e0p	184	13	for	for	IN
cord-265978-i0fu8e0p	184	14	proper	proper	JJ
cord-265978-i0fu8e0p	184	15	herd	herd	NN
cord-265978-i0fu8e0p	184	16	management	management	NN
cord-265978-i0fu8e0p	184	17	and	and	CC
cord-265978-i0fu8e0p	184	18	prevention	prevention	NN
cord-265978-i0fu8e0p	184	19	of	of	IN
cord-265978-i0fu8e0p	184	20	disease	disease	NN
cord-265978-i0fu8e0p	184	21	spread	spread	VBD
cord-265978-i0fu8e0p	184	22	.	.	.
cord-265978-i0fu8e0p	185	1	Moreover	moreover	RB
cord-265978-i0fu8e0p	185	2	,	,	,
cord-265978-i0fu8e0p	185	3	this	this	DT
cord-265978-i0fu8e0p	185	4	assay	assay	NN
cord-265978-i0fu8e0p	185	5	can	can	MD
cord-265978-i0fu8e0p	185	6	be	be	VB
cord-265978-i0fu8e0p	185	7	considered	consider	VBN
cord-265978-i0fu8e0p	185	8	as	as	IN
cord-265978-i0fu8e0p	185	9	a	a	DT
cord-265978-i0fu8e0p	185	10	suitable	suitable	JJ
cord-265978-i0fu8e0p	185	11	alternative	alternative	NN
cord-265978-i0fu8e0p	185	12	for	for	IN
cord-265978-i0fu8e0p	185	13	the	the	DT
cord-265978-i0fu8e0p	185	14	regular	regular	JJ
cord-265978-i0fu8e0p	185	15	methods	method	NNS
cord-265978-i0fu8e0p	185	16	used	use	VBN
cord-265978-i0fu8e0p	185	17	for	for	IN
cord-265978-i0fu8e0p	185	18	studying	study	VBG
cord-265978-i0fu8e0p	185	19	BCoV	BCoV	NNP
cord-265978-i0fu8e0p	185	20	pathogenesis	pathogenesis	NN
cord-265978-i0fu8e0p	185	21	and	and	CC
cord-265978-i0fu8e0p	185	22	vaccine	vaccine	NN
cord-265978-i0fu8e0p	185	23	trials	trial	NNS
cord-265978-i0fu8e0p	185	24	.	.	.
cord-265978-i0fu8e0p	186	1	H.M.	H.M.	NNP
cord-265978-i0fu8e0p	186	2	Amer	Amer	NNP
cord-265978-i0fu8e0p	186	3	,	,	,
cord-265978-i0fu8e0p	186	4	H.M.	H.M.	NNP
cord-265978-i0fu8e0p	186	5	designed	design	VBD
cord-265978-i0fu8e0p	186	6	the	the	DT
cord-265978-i0fu8e0p	186	7	experiments	experiment	NNS
cord-265978-i0fu8e0p	186	8	,	,	,
cord-265978-i0fu8e0p	186	9	performed	perform	VBD
cord-265978-i0fu8e0p	186	10	the	the	DT
cord-265978-i0fu8e0p	186	11	practical	practical	JJ
cord-265978-i0fu8e0p	186	12	work	work	NN
cord-265978-i0fu8e0p	186	13	and	and	CC
cord-265978-i0fu8e0p	186	14	revised	revise	VBD
cord-265978-i0fu8e0p	186	15	the	the	DT
cord-265978-i0fu8e0p	186	16	manuscript	manuscript	NN
cord-265978-i0fu8e0p	186	17	;	;	:
cord-265978-i0fu8e0p	186	18	F.N.	F.N.	NNP
cord-265978-i0fu8e0p	186	19	Almajhdi	Almajhdi	NNP
cord-265978-i0fu8e0p	186	20	developed	develop	VBD
cord-265978-i0fu8e0p	186	21	the	the	DT
cord-265978-i0fu8e0p	186	22	concept	concept	NN
cord-265978-i0fu8e0p	186	23	,	,	,
cord-265978-i0fu8e0p	186	24	interpret	interpret	VB
cord-265978-i0fu8e0p	186	25	the	the	DT
cord-265978-i0fu8e0p	186	26	results	result	NNS
cord-265978-i0fu8e0p	186	27	and	and	CC
cord-265978-i0fu8e0p	186	28	wrote	write	VBD
cord-265978-i0fu8e0p	186	29	the	the	DT
cord-265978-i0fu8e0p	186	30	manuscript	manuscript	NN
cord-265978-i0fu8e0p	186	31	.	.	.
cord-265978-i0fu8e0p	187	1	Enteric	enteric	JJ
cord-265978-i0fu8e0p	187	2	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	188	1	A	a	DT
cord-265978-i0fu8e0p	188	2	review	review	NN
cord-265978-i0fu8e0p	188	3	of	of	IN
cord-265978-i0fu8e0p	188	4	evidence	evidence	NN
cord-265978-i0fu8e0p	188	5	implicating	implicate	VBG
cord-265978-i0fu8e0p	188	6	Bovine	bovine	JJ
cord-265978-i0fu8e0p	188	7	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	188	8	in	in	IN
cord-265978-i0fu8e0p	188	9	the	the	DT
cord-265978-i0fu8e0p	188	10	etiology	etiology	NN
cord-265978-i0fu8e0p	188	11	of	of	IN
cord-265978-i0fu8e0p	188	12	winter	winter	NN
cord-265978-i0fu8e0p	188	13	dysentery	dysentery	NN
cord-265978-i0fu8e0p	188	14	in	in	IN
cord-265978-i0fu8e0p	188	15	cows	cow	NNS
cord-265978-i0fu8e0p	189	1	:	:	:
cord-265978-i0fu8e0p	189	2	an	an	DT
cord-265978-i0fu8e0p	189	3	enigma	enigma	NN
cord-265978-i0fu8e0p	189	4	resolved	resolve	VBN
cord-265978-i0fu8e0p	189	5	?	?	.
cord-265978-i0fu8e0p	190	1	Coronavirus	Coronavirus	NNP
cord-265978-i0fu8e0p	190	2	and	and	CC
cord-265978-i0fu8e0p	190	3	Pasteurella	Pasteurella	NNP
cord-265978-i0fu8e0p	190	4	infections	infection	NNS
cord-265978-i0fu8e0p	190	5	in	in	IN
cord-265978-i0fu8e0p	190	6	bovine	bovine	JJ
cord-265978-i0fu8e0p	190	7	shipping	shipping	NN
cord-265978-i0fu8e0p	190	8	fever	fever	NN
cord-265978-i0fu8e0p	190	9	pneumonia	pneumonia	NN
cord-265978-i0fu8e0p	190	10	and	and	CC
cord-265978-i0fu8e0p	190	11	Evans	Evans	NNP
cord-265978-i0fu8e0p	190	12	criteria	criterion	NNS
cord-265978-i0fu8e0p	190	13	for	for	IN
cord-265978-i0fu8e0p	190	14	causation	causation	NN
cord-265978-i0fu8e0p	190	15	Lactogenic	Lactogenic	NNP
cord-265978-i0fu8e0p	190	16	immunity	immunity	NN
cord-265978-i0fu8e0p	190	17	following	follow	VBG
cord-265978-i0fu8e0p	190	18	vaccination	vaccination	NN
cord-265978-i0fu8e0p	190	19	of	of	IN
cord-265978-i0fu8e0p	190	20	cattle	cattle	NNS
cord-265978-i0fu8e0p	190	21	with	with	IN
cord-265978-i0fu8e0p	190	22	Bovine	bovine	JJ
cord-265978-i0fu8e0p	190	23	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	190	24	Virus	Virus	NNP
cord-265978-i0fu8e0p	190	25	Taxonomy	Taxonomy	NNP
cord-265978-i0fu8e0p	190	26	.	.	.
cord-265978-i0fu8e0p	191	1	Classification	classification	NN
cord-265978-i0fu8e0p	191	2	and	and	CC
cord-265978-i0fu8e0p	191	3	nomenclature	nomenclature	NN
cord-265978-i0fu8e0p	191	4	of	of	IN
cord-265978-i0fu8e0p	191	5	viruses	virus	NNS
cord-265978-i0fu8e0p	191	6	.	.	.
cord-265978-i0fu8e0p	192	1	Seventh	seventh	JJ
cord-265978-i0fu8e0p	192	2	report	report	NN
cord-265978-i0fu8e0p	192	3	of	of	IN
cord-265978-i0fu8e0p	192	4	the	the	DT
cord-265978-i0fu8e0p	192	5	International	International	NNP
cord-265978-i0fu8e0p	192	6	Committee	Committee	NNP
cord-265978-i0fu8e0p	192	7	on	on	IN
cord-265978-i0fu8e0p	192	8	the	the	DT
cord-265978-i0fu8e0p	192	9	Taxonomy	Taxonomy	NNP
cord-265978-i0fu8e0p	192	10	of	of	IN
cord-265978-i0fu8e0p	192	11	viruses	virus	NNS
cord-265978-i0fu8e0p	193	1	Bovine	bovine	JJ
cord-265978-i0fu8e0p	193	2	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	193	3	genome	genome	NN
cord-265978-i0fu8e0p	194	1	The	the	DT
cord-265978-i0fu8e0p	194	2	molecular	molecular	JJ
cord-265978-i0fu8e0p	194	3	biology	biology	NN
cord-265978-i0fu8e0p	194	4	of	of	IN
cord-265978-i0fu8e0p	194	5	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	194	6	Sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	194	7	comparison	comparison	NN
cord-265978-i0fu8e0p	194	8	for	for	IN
cord-265978-i0fu8e0p	194	9	detection	detection	NN
cord-265978-i0fu8e0p	194	10	of	of	IN
cord-265978-i0fu8e0p	194	11	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	194	12	Bovine	bovine	JJ
cord-265978-i0fu8e0p	194	13	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	194	14	in	in	IN
cord-265978-i0fu8e0p	194	15	nasal	nasal	NN
cord-265978-i0fu8e0p	194	16	samples	sample	NNS
cord-265978-i0fu8e0p	194	17	from	from	IN
cord-265978-i0fu8e0p	194	18	feedlot	feedlot	NN
cord-265978-i0fu8e0p	194	19	cattle	cattle	NNS
cord-265978-i0fu8e0p	194	20	by	by	IN
cord-265978-i0fu8e0p	194	21	ELISA	ELISA	NNP
cord-265978-i0fu8e0p	194	22	and	and	CC
cord-265978-i0fu8e0p	194	23	isolation	isolation	NN
cord-265978-i0fu8e0p	194	24	with	with	IN
cord-265978-i0fu8e0p	194	25	the	the	DT
cord-265978-i0fu8e0p	194	26	G	g	NN
cord-265978-i0fu8e0p	194	27	clone	clone	NN
cord-265978-i0fu8e0p	194	28	of	of	IN
cord-265978-i0fu8e0p	194	29	HRT-18	HRT-18	NNP
cord-265978-i0fu8e0p	194	30	cells	cell	NNS
cord-265978-i0fu8e0p	194	31	Cross	Cross	NNP
cord-265978-i0fu8e0p	194	32	protection	protection	NN
cord-265978-i0fu8e0p	194	33	studies	study	NNS
cord-265978-i0fu8e0p	194	34	between	between	IN
cord-265978-i0fu8e0p	194	35	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	194	36	and	and	CC
cord-265978-i0fu8e0p	194	37	calf	calf	NN
cord-265978-i0fu8e0p	194	38	diarrhea	diarrhea	NN
cord-265978-i0fu8e0p	194	39	and	and	CC
cord-265978-i0fu8e0p	194	40	winter	winter	NN
cord-265978-i0fu8e0p	194	41	dysentery	dysentery	NN
cord-265978-i0fu8e0p	194	42	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	194	43	strains	strain	NNS
cord-265978-i0fu8e0p	194	44	in	in	IN
cord-265978-i0fu8e0p	194	45	calves	calf	NNS
cord-265978-i0fu8e0p	194	46	and	and	CC
cord-265978-i0fu8e0p	194	47	RT	RT	NNP
cord-265978-i0fu8e0p	194	48	-	-	HYPH
cord-265978-i0fu8e0p	194	49	PCR	PCR	NNP
cord-265978-i0fu8e0p	194	50	and	and	CC
cord-265978-i0fu8e0p	194	51	nested	nest	VBN
cord-265978-i0fu8e0p	194	52	PCR	PCR	NNP
cord-265978-i0fu8e0p	194	53	for	for	IN
cord-265978-i0fu8e0p	194	54	their	-PRON-	PRP$
cord-265978-i0fu8e0p	194	55	detection	detection	NN
cord-265978-i0fu8e0p	194	56	Improved	improve	VBN
cord-265978-i0fu8e0p	194	57	detection	detection	NN
cord-265978-i0fu8e0p	194	58	of	of	IN
cord-265978-i0fu8e0p	194	59	bovine	bovine	JJ
cord-265978-i0fu8e0p	194	60	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	194	61	N	n	CD
cord-265978-i0fu8e0p	194	62	gene	gene	NN
cord-265978-i0fu8e0p	194	63	in	in	IN
cord-265978-i0fu8e0p	194	64	faeces	faece	NNS
cord-265978-i0fu8e0p	194	65	of	of	IN
cord-265978-i0fu8e0p	194	66	calves	calf	NNS
cord-265978-i0fu8e0p	194	67	infected	infect	VBN
cord-265978-i0fu8e0p	194	68	naturally	naturally	RB
cord-265978-i0fu8e0p	194	69	by	by	IN
cord-265978-i0fu8e0p	194	70	a	a	DT
cord-265978-i0fu8e0p	194	71	semi	semi	RB
cord-265978-i0fu8e0p	194	72	-	-	JJ
cord-265978-i0fu8e0p	194	73	nested	nested	JJ
cord-265978-i0fu8e0p	194	74	PCR	PCR	NNP
cord-265978-i0fu8e0p	194	75	assay	assay	NN
cord-265978-i0fu8e0p	194	76	and	and	CC
cord-265978-i0fu8e0p	194	77	an	an	DT
cord-265978-i0fu8e0p	194	78	internal	internal	JJ
cord-265978-i0fu8e0p	194	79	control	control	NN
cord-265978-i0fu8e0p	194	80	Quantification	quantification	NN
cord-265978-i0fu8e0p	194	81	using	use	VBG
cord-265978-i0fu8e0p	194	82	real	real	JJ
cord-265978-i0fu8e0p	194	83	-	-	HYPH
cord-265978-i0fu8e0p	194	84	time	time	NN
cord-265978-i0fu8e0p	194	85	PCR	pcr	NN
cord-265978-i0fu8e0p	194	86	technology	technology	NN
cord-265978-i0fu8e0p	194	87	:	:	:
cord-265978-i0fu8e0p	194	88	applications	application	NNS
cord-265978-i0fu8e0p	194	89	and	and	CC
cord-265978-i0fu8e0p	194	90	limitations	limitation	NNS
cord-265978-i0fu8e0p	194	91	Detection	detection	NN
cord-265978-i0fu8e0p	194	92	and	and	CC
cord-265978-i0fu8e0p	194	93	monitoring	monitoring	NN
cord-265978-i0fu8e0p	194	94	of	of	IN
cord-265978-i0fu8e0p	194	95	virus	virus	NN
cord-265978-i0fu8e0p	194	96	infections	infection	NNS
cord-265978-i0fu8e0p	194	97	by	by	IN
cord-265978-i0fu8e0p	194	98	real	real	JJ
cord-265978-i0fu8e0p	194	99	-	-	HYPH
cord-265978-i0fu8e0p	194	100	time	time	NN
cord-265978-i0fu8e0p	194	101	PCR	PCR	NNP
cord-265978-i0fu8e0p	194	102	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	194	103	Green	Green	NNP
cord-265978-i0fu8e0p	194	104	real	real	JJ
cord-265978-i0fu8e0p	194	105	-	-	HYPH
cord-265978-i0fu8e0p	194	106	time	time	NN
cord-265978-i0fu8e0p	194	107	reverse	reverse	NN
cord-265978-i0fu8e0p	194	108	transcription	transcription	NN
cord-265978-i0fu8e0p	194	109	-	-	HYPH
cord-265978-i0fu8e0p	194	110	polymerase	polymerase	NN
cord-265978-i0fu8e0p	194	111	chain	chain	NN
cord-265978-i0fu8e0p	194	112	reaction	reaction	NN
cord-265978-i0fu8e0p	194	113	assay	assay	NN
cord-265978-i0fu8e0p	194	114	for	for	IN
cord-265978-i0fu8e0p	194	115	the	the	DT
cord-265978-i0fu8e0p	194	116	genetic	genetic	JJ
cord-265978-i0fu8e0p	194	117	detection	detection	NN
cord-265978-i0fu8e0p	194	118	of	of	IN
cord-265978-i0fu8e0p	194	119	coronaviruses	coronaviruse	NNS
cord-265978-i0fu8e0p	195	1	Baculovirus	Baculovirus	NNP
cord-265978-i0fu8e0p	195	2	expression	expression	NN
cord-265978-i0fu8e0p	195	3	of	of	IN
cord-265978-i0fu8e0p	195	4	the	the	DT
cord-265978-i0fu8e0p	195	5	Bovine	bovine	JJ
cord-265978-i0fu8e0p	195	6	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	195	7	nucleocapsid	nucleocapsid	NN
cord-265978-i0fu8e0p	195	8	protein	protein	NN
cord-265978-i0fu8e0p	195	9	in	in	IN
cord-265978-i0fu8e0p	195	10	Spodoptera	Spodoptera	NNP
cord-265978-i0fu8e0p	195	11	frugiperda	frugiperda	JJ
cord-265978-i0fu8e0p	195	12	insect	insect	NN
cord-265978-i0fu8e0p	195	13	cells	cell	NNS
cord-265978-i0fu8e0p	195	14	Development	Development	NNP
cord-265978-i0fu8e0p	195	15	of	of	IN
cord-265978-i0fu8e0p	195	16	a	a	DT
cord-265978-i0fu8e0p	195	17	quantitative	quantitative	JJ
cord-265978-i0fu8e0p	195	18	Light	Light	NNP
cord-265978-i0fu8e0p	195	19	Cycler	Cycler	NNP
cord-265978-i0fu8e0p	195	20	real	real	JJ
cord-265978-i0fu8e0p	195	21	-	-	HYPH
cord-265978-i0fu8e0p	195	22	time	time	NN
cord-265978-i0fu8e0p	195	23	RT	RT	NNP
cord-265978-i0fu8e0p	195	24	-	-	HYPH
cord-265978-i0fu8e0p	195	25	PCR	PCR	NNP
cord-265978-i0fu8e0p	195	26	for	for	IN
cord-265978-i0fu8e0p	195	27	detection	detection	NN
cord-265978-i0fu8e0p	195	28	of	of	IN
cord-265978-i0fu8e0p	195	29	avian	avian	JJ
cord-265978-i0fu8e0p	195	30	reovirus	reovirus	NNP
cord-265978-i0fu8e0p	195	31	Cell	cell	NN
cord-265978-i0fu8e0p	195	32	culture	culture	NN
cord-265978-i0fu8e0p	195	33	propagation	propagation	NN
cord-265978-i0fu8e0p	195	34	of	of	IN
cord-265978-i0fu8e0p	195	35	Bovine	bovine	JJ
cord-265978-i0fu8e0p	195	36	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	195	37	Real	real	JJ
cord-265978-i0fu8e0p	195	38	-	-	HYPH
cord-265978-i0fu8e0p	195	39	time	time	NN
cord-265978-i0fu8e0p	195	40	PCR	PCR	NNP
cord-265978-i0fu8e0p	195	41	in	in	IN
cord-265978-i0fu8e0p	195	42	the	the	DT
cord-265978-i0fu8e0p	195	43	microbiology	microbiology	NN
cord-265978-i0fu8e0p	195	44	laboratory	laboratory	NN
cord-265978-i0fu8e0p	195	45	Sequence	Sequence	NNP
cord-265978-i0fu8e0p	195	46	analysis	analysis	NN
cord-265978-i0fu8e0p	195	47	of	of	IN
cord-265978-i0fu8e0p	195	48	the	the	DT
cord-265978-i0fu8e0p	195	49	bovine	bovine	JJ
cord-265978-i0fu8e0p	195	50	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	195	51	nucleocapsid	nucleocapsid	NN
cord-265978-i0fu8e0p	195	52	and	and	CC
cord-265978-i0fu8e0p	195	53	matrix	matrix	NN
cord-265978-i0fu8e0p	195	54	protein	protein	NN
cord-265978-i0fu8e0p	195	55	genes	gene	NNS
cord-265978-i0fu8e0p	195	56	Coronaviruses	Coronaviruses	NNPS
cord-265978-i0fu8e0p	195	57	:	:	:
cord-265978-i0fu8e0p	196	1	structure	structure	NN
cord-265978-i0fu8e0p	196	2	and	and	CC
cord-265978-i0fu8e0p	196	3	genome	genome	NN
cord-265978-i0fu8e0p	196	4	expression	expression	NN
cord-265978-i0fu8e0p	196	5	Detection	detection	NN
cord-265978-i0fu8e0p	196	6	of	of	IN
cord-265978-i0fu8e0p	196	7	a	a	DT
cord-265978-i0fu8e0p	196	8	group	group	NN
cord-265978-i0fu8e0p	196	9	2	2	CD
cord-265978-i0fu8e0p	196	10	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	196	11	in	in	IN
cord-265978-i0fu8e0p	196	12	dogs	dog	NNS
cord-265978-i0fu8e0p	196	13	with	with	IN
cord-265978-i0fu8e0p	196	14	canine	canine	JJ
cord-265978-i0fu8e0p	196	15	infectious	infectious	JJ
cord-265978-i0fu8e0p	196	16	respiratory	respiratory	JJ
cord-265978-i0fu8e0p	196	17	disease	disease	NN
cord-265978-i0fu8e0p	196	18	Complete	complete	JJ
cord-265978-i0fu8e0p	196	19	genomic	genomic	JJ
cord-265978-i0fu8e0p	196	20	sequence	sequence	NN
cord-265978-i0fu8e0p	196	21	of	of	IN
cord-265978-i0fu8e0p	196	22	human	human	JJ
cord-265978-i0fu8e0p	196	23	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	196	24	OC43	OC43	NNP
cord-265978-i0fu8e0p	196	25	:	:	:
cord-265978-i0fu8e0p	196	26	molecular	molecular	JJ
cord-265978-i0fu8e0p	196	27	clock	clock	NN
cord-265978-i0fu8e0p	196	28	analysis	analysis	NN
cord-265978-i0fu8e0p	196	29	suggests	suggest	VBZ
cord-265978-i0fu8e0p	196	30	a	a	DT
cord-265978-i0fu8e0p	196	31	relatively	relatively	RB
cord-265978-i0fu8e0p	196	32	recent	recent	JJ
cord-265978-i0fu8e0p	196	33	zoonotic	zoonotic	JJ
cord-265978-i0fu8e0p	196	34	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	196	35	transmission	transmission	NN
cord-265978-i0fu8e0p	196	36	event	event	NN
cord-265978-i0fu8e0p	196	37	Biologic	Biologic	NNP
cord-265978-i0fu8e0p	196	38	,	,	,
cord-265978-i0fu8e0p	196	39	antigenic	antigenic	JJ
cord-265978-i0fu8e0p	196	40	,	,	,
cord-265978-i0fu8e0p	196	41	and	and	CC
cord-265978-i0fu8e0p	196	42	full	full	JJ
cord-265978-i0fu8e0p	196	43	-	-	HYPH
cord-265978-i0fu8e0p	196	44	length	length	NN
cord-265978-i0fu8e0p	196	45	genomic	genomic	JJ
cord-265978-i0fu8e0p	196	46	characterization	characterization	NN
cord-265978-i0fu8e0p	196	47	of	of	IN
cord-265978-i0fu8e0p	196	48	a	a	DT
cord-265978-i0fu8e0p	196	49	bovine	bovine	NN
cord-265978-i0fu8e0p	196	50	-	-	HYPH
cord-265978-i0fu8e0p	196	51	like	like	JJ
cord-265978-i0fu8e0p	196	52	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	196	53	isolated	isolate	VBN
cord-265978-i0fu8e0p	196	54	from	from	IN
cord-265978-i0fu8e0p	196	55	a	a	DT
cord-265978-i0fu8e0p	196	56	giraffe	giraffe	NN
cord-265978-i0fu8e0p	196	57	Real	real	JJ
cord-265978-i0fu8e0p	196	58	-	-	HYPH
cord-265978-i0fu8e0p	196	59	Time	time	NN
cord-265978-i0fu8e0p	196	60	PCR	PCR	NNP
cord-265978-i0fu8e0p	196	61	in	in	IN
cord-265978-i0fu8e0p	196	62	clinical	clinical	JJ
cord-265978-i0fu8e0p	196	63	microbiology	microbiology	NN
cord-265978-i0fu8e0p	196	64	:	:	:
cord-265978-i0fu8e0p	196	65	applications	application	NNS
cord-265978-i0fu8e0p	196	66	for	for	IN
cord-265978-i0fu8e0p	196	67	routine	routine	JJ
cord-265978-i0fu8e0p	196	68	laboratory	laboratory	NN
cord-265978-i0fu8e0p	196	69	testing	test	VBG
cord-265978-i0fu8e0p	196	70	Verification	verification	NN
cord-265978-i0fu8e0p	196	71	and	and	CC
cord-265978-i0fu8e0p	196	72	validation	validation	NN
cord-265978-i0fu8e0p	196	73	of	of	IN
cord-265978-i0fu8e0p	196	74	diagnostic	diagnostic	JJ
cord-265978-i0fu8e0p	196	75	laboratory	laboratory	NN
cord-265978-i0fu8e0p	196	76	tests	test	NNS
cord-265978-i0fu8e0p	196	77	in	in	IN
cord-265978-i0fu8e0p	196	78	clinical	clinical	JJ
cord-265978-i0fu8e0p	196	79	virology	virology	NN
cord-265978-i0fu8e0p	196	80	Complex	complex	JJ
cord-265978-i0fu8e0p	196	81	polysaccharides	polysaccharide	NNS
cord-265978-i0fu8e0p	196	82	as	as	IN
cord-265978-i0fu8e0p	196	83	PCR	PCR	NNP
cord-265978-i0fu8e0p	196	84	inhibitors	inhibitor	NNS
cord-265978-i0fu8e0p	196	85	in	in	IN
cord-265978-i0fu8e0p	196	86	feces	fece	NNS
cord-265978-i0fu8e0p	196	87	:	:	:
cord-265978-i0fu8e0p	196	88	Helicobacter	Helicobacter	NNP
cord-265978-i0fu8e0p	196	89	pylori	pylori	NN
cord-265978-i0fu8e0p	196	90	model	model	NN
cord-265978-i0fu8e0p	197	1	Practical	practical	JJ
cord-265978-i0fu8e0p	197	2	considerations	consideration	NNS
cord-265978-i0fu8e0p	197	3	in	in	IN
cord-265978-i0fu8e0p	197	4	design	design	NN
cord-265978-i0fu8e0p	197	5	of	of	IN
cord-265978-i0fu8e0p	197	6	internal	internal	JJ
cord-265978-i0fu8e0p	197	7	amplification	amplification	NN
cord-265978-i0fu8e0p	197	8	controls	control	NNS
cord-265978-i0fu8e0p	197	9	for	for	IN
cord-265978-i0fu8e0p	197	10	diagnostic	diagnostic	JJ
cord-265978-i0fu8e0p	197	11	PCR	PCR	NNP
cord-265978-i0fu8e0p	197	12	assays	assay	NNS
cord-265978-i0fu8e0p	198	1	The	the	DT
cord-265978-i0fu8e0p	198	2	real	real	JJ
cord-265978-i0fu8e0p	198	3	time	time	NN
cord-265978-i0fu8e0p	198	4	polymerase	polymerase	NN
cord-265978-i0fu8e0p	198	5	chain	chain	NN
cord-265978-i0fu8e0p	198	6	reaction	reaction	NN
cord-265978-i0fu8e0p	198	7	Differential	differential	JJ
cord-265978-i0fu8e0p	198	8	amplification	amplification	NN
cord-265978-i0fu8e0p	198	9	and	and	CC
cord-265978-i0fu8e0p	198	10	quantitation	quantitation	NN
cord-265978-i0fu8e0p	198	11	of	of	IN
cord-265978-i0fu8e0p	198	12	Marek	Marek	NNP
cord-265978-i0fu8e0p	198	13	's	's	POS
cord-265978-i0fu8e0p	198	14	disease	disease	NN
cord-265978-i0fu8e0p	198	15	viruses	virus	NNS
cord-265978-i0fu8e0p	198	16	using	use	VBG
cord-265978-i0fu8e0p	198	17	real	real	JJ
cord-265978-i0fu8e0p	198	18	-	-	HYPH
cord-265978-i0fu8e0p	198	19	time	time	NN
cord-265978-i0fu8e0p	198	20	polymerase	polymerase	NN
cord-265978-i0fu8e0p	198	21	chain	chain	NN
cord-265978-i0fu8e0p	198	22	reaction	reaction	NN
cord-265978-i0fu8e0p	198	23	Detection	detection	NN
cord-265978-i0fu8e0p	198	24	of	of	IN
cord-265978-i0fu8e0p	198	25	bovine	bovine	JJ
cord-265978-i0fu8e0p	198	26	coronavirus	coronavirus	NN
cord-265978-i0fu8e0p	198	27	using	use	VBG
cord-265978-i0fu8e0p	198	28	a	a	DT
cord-265978-i0fu8e0p	198	29	TaqMan	TaqMan	NNP
cord-265978-i0fu8e0p	198	30	-	-	HYPH
cord-265978-i0fu8e0p	198	31	based	base	VBN
cord-265978-i0fu8e0p	198	32	real	real	JJ
cord-265978-i0fu8e0p	198	33	-	-	HYPH
cord-265978-i0fu8e0p	198	34	time	time	NN
cord-265978-i0fu8e0p	198	35	RT	RT	NNP
cord-265978-i0fu8e0p	198	36	-	-	HYPH
cord-265978-i0fu8e0p	198	37	PCR	PCR	NNP
cord-265978-i0fu8e0p	198	38	assay	assay	NN
cord-265978-i0fu8e0p	198	39	Development	Development	NNP
cord-265978-i0fu8e0p	198	40	of	of	IN
cord-265978-i0fu8e0p	198	41	a	a	DT
cord-265978-i0fu8e0p	198	42	panel	panel	NN
cord-265978-i0fu8e0p	198	43	of	of	IN
cord-265978-i0fu8e0p	198	44	multiplex	multiplex	JJ
cord-265978-i0fu8e0p	198	45	real	real	JJ
cord-265978-i0fu8e0p	198	46	-	-	HYPH
cord-265978-i0fu8e0p	198	47	time	time	NN
cord-265978-i0fu8e0p	198	48	polymerase	polymerase	NN
cord-265978-i0fu8e0p	198	49	chain	chain	NN
cord-265978-i0fu8e0p	198	50	reaction	reaction	NN
cord-265978-i0fu8e0p	198	51	assays	assay	NNS
cord-265978-i0fu8e0p	198	52	for	for	IN
cord-265978-i0fu8e0p	198	53	simultaneous	simultaneous	JJ
cord-265978-i0fu8e0p	198	54	detection	detection	NN
cord-265978-i0fu8e0p	198	55	of	of	IN
cord-265978-i0fu8e0p	198	56	major	major	JJ
cord-265978-i0fu8e0p	198	57	agents	agent	NNS
cord-265978-i0fu8e0p	198	58	causing	cause	VBG
cord-265978-i0fu8e0p	198	59	calf	calf	NN
cord-265978-i0fu8e0p	198	60	diarrhea	diarrhea	NN
cord-265978-i0fu8e0p	198	61	in	in	IN
cord-265978-i0fu8e0p	198	62	feces	fece	NNS
cord-265978-i0fu8e0p	198	63	Rapid	Rapid	NNP
cord-265978-i0fu8e0p	198	64	detection	detection	NN
cord-265978-i0fu8e0p	198	65	of	of	IN
cord-265978-i0fu8e0p	198	66	herpes	herpes	NNP
cord-265978-i0fu8e0p	198	67	simplex	simplex	NNP
cord-265978-i0fu8e0p	198	68	virus	virus	NN
cord-265978-i0fu8e0p	198	69	DNA	dna	NN
cord-265978-i0fu8e0p	198	70	in	in	IN
cord-265978-i0fu8e0p	198	71	genital	genital	JJ
cord-265978-i0fu8e0p	198	72	ulcers	ulcer	NNS
cord-265978-i0fu8e0p	198	73	by	by	IN
cord-265978-i0fu8e0p	198	74	real	real	JJ
cord-265978-i0fu8e0p	198	75	-	-	HYPH
cord-265978-i0fu8e0p	198	76	time	time	NN
cord-265978-i0fu8e0p	198	77	PCR	pcr	NN
cord-265978-i0fu8e0p	198	78	using	use	VBG
cord-265978-i0fu8e0p	198	79	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	198	80	Green	Green	NNP
cord-265978-i0fu8e0p	199	1	I	-PRON-	PRP
cord-265978-i0fu8e0p	199	2	dye	dye	VBP
cord-265978-i0fu8e0p	199	3	as	as	IN
cord-265978-i0fu8e0p	199	4	the	the	DT
cord-265978-i0fu8e0p	199	5	detection	detection	NN
cord-265978-i0fu8e0p	199	6	signal	signal	NN
cord-265978-i0fu8e0p	199	7	SYBR	SYBR	NNP
cord-265978-i0fu8e0p	199	8	Green	Green	NNP
cord-265978-i0fu8e0p	200	1	I	-PRON-	PRP
cord-265978-i0fu8e0p	200	2	DNA	dna	NN
cord-265978-i0fu8e0p	200	3	staining	staining	NN
cord-265978-i0fu8e0p	200	4	increases	increase	VBZ
cord-265978-i0fu8e0p	200	5	the	the	DT
cord-265978-i0fu8e0p	200	6	detection	detection	NN
cord-265978-i0fu8e0p	200	7	sensitivity	sensitivity	NN
cord-265978-i0fu8e0p	200	8	of	of	IN
cord-265978-i0fu8e0p	200	9	viruses	virus	NNS
cord-265978-i0fu8e0p	200	10	by	by	IN
cord-265978-i0fu8e0p	200	11	polymerase	polymerase	NN
cord-265978-i0fu8e0p	200	12	chain	chain	NN
cord-265978-i0fu8e0p	200	13	reaction	reaction	NN