id	sid	tid	token	lemma	pos
cord-017363-dmb42kna	1	1	key	key	NN
cord-017363-dmb42kna	1	2	:	:	:
cord-017363-dmb42kna	1	3	cord-017363-dmb42kna	cord-017363-dmb42kna	NN
cord-017363-dmb42kna	1	4	authors	author	NNS
cord-017363-dmb42kna	1	5	:	:	:
cord-017363-dmb42kna	1	6	Vemulapalli	Vemulapalli	NNP
cord-017363-dmb42kna	1	7	,	,	,
cord-017363-dmb42kna	1	8	Ramesh	Ramesh	NNP
cord-017363-dmb42kna	1	9	title	title	NN
cord-017363-dmb42kna	1	10	:	:	:
cord-017363-dmb42kna	1	11	Real	real	JJ
cord-017363-dmb42kna	1	12	-	-	HYPH
cord-017363-dmb42kna	1	13	Time	time	NN
cord-017363-dmb42kna	1	14	Reverse	Reverse	NNP
cord-017363-dmb42kna	1	15	Transcription	Transcription	NNP
cord-017363-dmb42kna	1	16	Polymerase	Polymerase	NNP
cord-017363-dmb42kna	1	17	Chain	Chain	NNP
cord-017363-dmb42kna	1	18	Reaction	reaction	NN
cord-017363-dmb42kna	1	19	for	for	IN
cord-017363-dmb42kna	1	20	Rapid	Rapid	NNP
cord-017363-dmb42kna	1	21	Detection	Detection	NNP
cord-017363-dmb42kna	1	22	of	of	IN
cord-017363-dmb42kna	1	23	Transmissible	transmissible	JJ
cord-017363-dmb42kna	1	24	Gastroenteritis	Gastroenteritis	NNP
cord-017363-dmb42kna	1	25	Virus	Virus	NNP
cord-017363-dmb42kna	1	26	date	date	NN
cord-017363-dmb42kna	1	27	:	:	:
cord-017363-dmb42kna	1	28	2015	2015	CD
cord-017363-dmb42kna	1	29	-	-	HYPH
cord-017363-dmb42kna	1	30	09	09	CD
cord-017363-dmb42kna	1	31	-	-	SYM
cord-017363-dmb42kna	1	32	10	10	CD
cord-017363-dmb42kna	1	33	journal	journal	NN
cord-017363-dmb42kna	1	34	:	:	:
cord-017363-dmb42kna	2	1	Animal	Animal	NNP
cord-017363-dmb42kna	2	2	Coronaviruses	Coronaviruses	NNP
cord-017363-dmb42kna	2	3	DOI	DOI	NNP
cord-017363-dmb42kna	2	4	:	:	:
cord-017363-dmb42kna	2	5	10.1007/978	10.1007/978	CD
cord-017363-dmb42kna	2	6	-	-	HYPH
cord-017363-dmb42kna	2	7	1	1	CD
cord-017363-dmb42kna	2	8	-	-	HYPH
cord-017363-dmb42kna	2	9	4939	4939	CD
cord-017363-dmb42kna	2	10	-	-	HYPH
cord-017363-dmb42kna	2	11	3414	3414	CD
cord-017363-dmb42kna	2	12	-	-	HYPH
cord-017363-dmb42kna	2	13	0_10	0_10	CD
cord-017363-dmb42kna	3	1	sha	sha	NNP
cord-017363-dmb42kna	3	2	:	:	:
cord-017363-dmb42kna	4	1	9e8479f3f693499f502384fd441ea492768775c6	9e8479f3f693499f502384fd441ea492768775c6	NNP
cord-017363-dmb42kna	4	2	doc_id	doc_id	CD
cord-017363-dmb42kna	4	3	:	:	:
cord-017363-dmb42kna	4	4	17363	17363	CD
cord-017363-dmb42kna	4	5	cord_uid	cord_uid	NNS
cord-017363-dmb42kna	4	6	:	:	:
cord-017363-dmb42kna	5	1	dmb42kna	dmb42kna	NNS
cord-017363-dmb42kna	6	1	Transmissible	transmissible	JJ
cord-017363-dmb42kna	6	2	gastroenteritis	gastroenteritis	NN
cord-017363-dmb42kna	6	3	(	(	-LRB-
cord-017363-dmb42kna	6	4	TGE	TGE	NNP
cord-017363-dmb42kna	6	5	)	)	-RRB-
cord-017363-dmb42kna	6	6	is	be	VBZ
cord-017363-dmb42kna	6	7	a	a	DT
cord-017363-dmb42kna	6	8	highly	highly	RB
cord-017363-dmb42kna	6	9	contagious	contagious	JJ
cord-017363-dmb42kna	6	10	disease	disease	NN
cord-017363-dmb42kna	6	11	of	of	IN
cord-017363-dmb42kna	6	12	pigs	pig	NNS
cord-017363-dmb42kna	6	13	caused	cause	VBN
cord-017363-dmb42kna	6	14	by	by	IN
cord-017363-dmb42kna	6	15	the	the	DT
cord-017363-dmb42kna	6	16	TGE	TGE	NNP
cord-017363-dmb42kna	6	17	virus	virus	NN
cord-017363-dmb42kna	6	18	(	(	-LRB-
cord-017363-dmb42kna	6	19	TGEV	TGEV	NNP
cord-017363-dmb42kna	6	20	)	)	-RRB-
cord-017363-dmb42kna	6	21	.	.	.
cord-017363-dmb42kna	7	1	Rapid	rapid	JJ
cord-017363-dmb42kna	7	2	detection	detection	NN
cord-017363-dmb42kna	7	3	of	of	IN
cord-017363-dmb42kna	7	4	the	the	DT
cord-017363-dmb42kna	7	5	virus	virus	NN
cord-017363-dmb42kna	7	6	in	in	IN
cord-017363-dmb42kna	7	7	the	the	DT
cord-017363-dmb42kna	7	8	affected	affected	JJ
cord-017363-dmb42kna	7	9	pigs	pig	NNS
cord-017363-dmb42kna	7	10	’	'	''
cord-017363-dmb42kna	7	11	feces	fece	NNS
cord-017363-dmb42kna	7	12	is	be	VBZ
cord-017363-dmb42kna	7	13	critical	critical	JJ
cord-017363-dmb42kna	7	14	for	for	IN
cord-017363-dmb42kna	7	15	controlling	control	VBG
cord-017363-dmb42kna	7	16	the	the	DT
cord-017363-dmb42kna	7	17	disease	disease	NN
cord-017363-dmb42kna	7	18	outbreaks	outbreak	NNS
cord-017363-dmb42kna	7	19	.	.	.
cord-017363-dmb42kna	8	1	The	the	DT
cord-017363-dmb42kna	8	2	real	real	JJ
cord-017363-dmb42kna	8	3	-	-	HYPH
cord-017363-dmb42kna	8	4	time	time	NN
cord-017363-dmb42kna	8	5	RT	RT	NNP
cord-017363-dmb42kna	8	6	-	-	HYPH
cord-017363-dmb42kna	8	7	PCR	PCR	NNP
cord-017363-dmb42kna	8	8	assay	assay	NN
cord-017363-dmb42kna	8	9	described	describe	VBN
cord-017363-dmb42kna	8	10	in	in	IN
cord-017363-dmb42kna	8	11	this	this	DT
cord-017363-dmb42kna	8	12	chapter	chapter	NN
cord-017363-dmb42kna	8	13	can	can	MD
cord-017363-dmb42kna	8	14	quickly	quickly	RB
cord-017363-dmb42kna	8	15	detect	detect	VB
cord-017363-dmb42kna	8	16	the	the	DT
cord-017363-dmb42kna	8	17	presence	presence	NN
cord-017363-dmb42kna	8	18	of	of	IN
cord-017363-dmb42kna	8	19	TGEV	TGEV	NNP
cord-017363-dmb42kna	8	20	in	in	IN
cord-017363-dmb42kna	8	21	fecal	fecal	JJ
cord-017363-dmb42kna	8	22	samples	sample	NNS
cord-017363-dmb42kna	8	23	with	with	IN
cord-017363-dmb42kna	8	24	high	high	JJ
cord-017363-dmb42kna	8	25	sensitivity	sensitivity	NN
cord-017363-dmb42kna	8	26	and	and	CC
cord-017363-dmb42kna	8	27	specificity	specificity	NN
cord-017363-dmb42kna	8	28	.	.	.
cord-017363-dmb42kna	9	1	Transmissible	transmissible	JJ
cord-017363-dmb42kna	9	2	gastroenteritis	gastroenteritis	NN
cord-017363-dmb42kna	9	3	(	(	-LRB-
cord-017363-dmb42kna	9	4	TGE	TGE	NNP
cord-017363-dmb42kna	9	5	)	)	-RRB-
cord-017363-dmb42kna	9	6	is	be	VBZ
cord-017363-dmb42kna	9	7	a	a	DT
cord-017363-dmb42kna	9	8	highly	highly	RB
cord-017363-dmb42kna	9	9	contagious	contagious	JJ
cord-017363-dmb42kna	9	10	,	,	,
cord-017363-dmb42kna	9	11	acute	acute	JJ
cord-017363-dmb42kna	9	12	viral	viral	JJ
cord-017363-dmb42kna	9	13	disease	disease	NN
cord-017363-dmb42kna	9	14	of	of	IN
cord-017363-dmb42kna	9	15	pigs	pig	NNS
cord-017363-dmb42kna	9	16	[	[	-LRB-
cord-017363-dmb42kna	9	17	1	1	CD
cord-017363-dmb42kna	9	18	]	]	-RRB-
cord-017363-dmb42kna	9	19	.	.	.
cord-017363-dmb42kna	10	1	TGE	TGE	NNP
cord-017363-dmb42kna	10	2	can	can	MD
cord-017363-dmb42kna	10	3	affect	affect	VB
cord-017363-dmb42kna	10	4	pigs	pig	NNS
cord-017363-dmb42kna	10	5	of	of	IN
cord-017363-dmb42kna	10	6	all	all	DT
cord-017363-dmb42kna	10	7	ages	age	NNS
cord-017363-dmb42kna	10	8	,	,	,
cord-017363-dmb42kna	10	9	but	but	CC
cord-017363-dmb42kna	10	10	the	the	DT
cord-017363-dmb42kna	10	11	disease	disease	NN
cord-017363-dmb42kna	10	12	severity	severity	NN
cord-017363-dmb42kna	10	13	and	and	CC
cord-017363-dmb42kna	10	14	mortality	mortality	NN
cord-017363-dmb42kna	10	15	rate	rate	NN
cord-017363-dmb42kna	10	16	are	be	VBP
cord-017363-dmb42kna	10	17	high	high	JJ
cord-017363-dmb42kna	10	18	in	in	IN
cord-017363-dmb42kna	10	19	piglets	piglet	NNS
cord-017363-dmb42kna	10	20	under	under	IN
cord-017363-dmb42kna	10	21	2	2	CD
cord-017363-dmb42kna	10	22	weeks	week	NNS
cord-017363-dmb42kna	10	23	of	of	IN
cord-017363-dmb42kna	10	24	age	age	NN
cord-017363-dmb42kna	10	25	.	.	.
cord-017363-dmb42kna	11	1	The	the	DT
cord-017363-dmb42kna	11	2	causative	causative	JJ
cord-017363-dmb42kna	11	3	agent	agent	NN
cord-017363-dmb42kna	11	4	is	be	VBZ
cord-017363-dmb42kna	11	5	TGE	TGE	NNP
cord-017363-dmb42kna	11	6	virus	virus	NN
cord-017363-dmb42kna	11	7	(	(	-LRB-
cord-017363-dmb42kna	11	8	TGEV	TGEV	NNP
cord-017363-dmb42kna	11	9	)	)	-RRB-
cord-017363-dmb42kna	11	10	,	,	,
cord-017363-dmb42kna	11	11	a	a	DT
cord-017363-dmb42kna	11	12	coronavirus	coronavirus	NN
cord-017363-dmb42kna	11	13	that	that	WDT
cord-017363-dmb42kna	11	14	primarily	primarily	RB
cord-017363-dmb42kna	11	15	infects	infect	VBZ
cord-017363-dmb42kna	11	16	and	and	CC
cord-017363-dmb42kna	11	17	replicates	replicate	VBZ
cord-017363-dmb42kna	11	18	in	in	IN
cord-017363-dmb42kna	11	19	the	the	DT
cord-017363-dmb42kna	11	20	epithelial	epithelial	JJ
cord-017363-dmb42kna	11	21	cells	cell	NNS
cord-017363-dmb42kna	11	22	of	of	IN
cord-017363-dmb42kna	11	23	pig	pig	NN
cord-017363-dmb42kna	11	24	intestines	intestine	NNS
cord-017363-dmb42kna	11	25	.	.	.
cord-017363-dmb42kna	12	1	Affected	affected	JJ
cord-017363-dmb42kna	12	2	animals	animal	NNS
cord-017363-dmb42kna	12	3	shed	shed	VBD
cord-017363-dmb42kna	12	4	the	the	DT
cord-017363-dmb42kna	12	5	virus	virus	NN
cord-017363-dmb42kna	12	6	in	in	IN
cord-017363-dmb42kna	12	7	their	-PRON-	PRP$
cord-017363-dmb42kna	12	8	feces	fece	NNS
cord-017363-dmb42kna	12	9	.	.	.
cord-017363-dmb42kna	13	1	The	the	DT
cord-017363-dmb42kna	13	2	disease	disease	NN
cord-017363-dmb42kna	13	3	transmission	transmission	NN
cord-017363-dmb42kna	13	4	occurs	occur	VBZ
cord-017363-dmb42kna	13	5	primarily	primarily	RB
cord-017363-dmb42kna	13	6	via	via	IN
cord-017363-dmb42kna	13	7	fecal	fecal	JJ
cord-017363-dmb42kna	13	8	-	-	HYPH
cord-017363-dmb42kna	13	9	oral	oral	JJ
cord-017363-dmb42kna	13	10	route	route	NN
cord-017363-dmb42kna	13	11	[	[	-LRB-
cord-017363-dmb42kna	13	12	2	2	CD
cord-017363-dmb42kna	13	13	]	]	-RRB-
cord-017363-dmb42kna	13	14	.	.	.
cord-017363-dmb42kna	14	1	During	during	IN
cord-017363-dmb42kna	14	2	an	an	DT
cord-017363-dmb42kna	14	3	outbreak	outbreak	NN
cord-017363-dmb42kna	14	4	,	,	,
cord-017363-dmb42kna	14	5	rapid	rapid	JJ
cord-017363-dmb42kna	14	6	detection	detection	NN
cord-017363-dmb42kna	14	7	of	of	IN
cord-017363-dmb42kna	14	8	TGEV	TGEV	NNP
cord-017363-dmb42kna	14	9	in	in	IN
cord-017363-dmb42kna	14	10	feces	fece	NNS
cord-017363-dmb42kna	14	11	is	be	VBZ
cord-017363-dmb42kna	14	12	very	very	RB
cord-017363-dmb42kna	14	13	useful	useful	JJ
cord-017363-dmb42kna	14	14	for	for	IN
cord-017363-dmb42kna	14	15	implementing	implement	VBG
cord-017363-dmb42kna	14	16	the	the	DT
cord-017363-dmb42kna	14	17	disease	disease	NN
cord-017363-dmb42kna	14	18	management	management	NN
cord-017363-dmb42kna	14	19	practices	practice	NNS
cord-017363-dmb42kna	14	20	in	in	IN
cord-017363-dmb42kna	14	21	a	a	DT
cord-017363-dmb42kna	14	22	timely	timely	JJ
cord-017363-dmb42kna	14	23	manner	manner	NN
cord-017363-dmb42kna	14	24	.	.	.
cord-017363-dmb42kna	15	1	Any	any	DT
cord-017363-dmb42kna	15	2	TGEV	TGEV	NNP
cord-017363-dmb42kna	15	3	-	-	HYPH
cord-017363-dmb42kna	15	4	specifi	specifi	NNP
cord-017363-dmb42kna	15	5	c	c	NN
cord-017363-dmb42kna	15	6	diagnostic	diagnostic	JJ
cord-017363-dmb42kna	15	7	assay	assay	NN
cord-017363-dmb42kna	15	8	must	must	MD
cord-017363-dmb42kna	15	9	be	be	VB
cord-017363-dmb42kna	15	10	able	able	JJ
cord-017363-dmb42kna	15	11	to	to	TO
cord-017363-dmb42kna	15	12	differentiate	differentiate	VB
cord-017363-dmb42kna	15	13	it	-PRON-	PRP
cord-017363-dmb42kna	15	14	from	from	IN
cord-017363-dmb42kna	15	15	porcine	porcine	JJ
cord-017363-dmb42kna	15	16	respiratory	respiratory	JJ
cord-017363-dmb42kna	15	17	coronavirus	coronavirus	NN
cord-017363-dmb42kna	15	18	(	(	-LRB-
cord-017363-dmb42kna	15	19	PRCV	PRCV	NNP
cord-017363-dmb42kna	15	20	)	)	-RRB-
cord-017363-dmb42kna	15	21	,	,	,
cord-017363-dmb42kna	15	22	a	a	DT
cord-017363-dmb42kna	15	23	natural	natural	JJ
cord-017363-dmb42kna	15	24	mutant	mutant	NN
cord-017363-dmb42kna	15	25	of	of	IN
cord-017363-dmb42kna	15	26	TGEV	TGEV	NNP
cord-017363-dmb42kna	15	27	with	with	IN
cord-017363-dmb42kna	15	28	truncated	truncated	JJ
cord-017363-dmb42kna	15	29	spike	spike	NN
cord-017363-dmb42kna	15	30	protein	protein	NN
cord-017363-dmb42kna	15	31	and	and	CC
cord-017363-dmb42kna	15	32	altered	altered	JJ
cord-017363-dmb42kna	15	33	cell	cell	NN
cord-017363-dmb42kna	15	34	tropism	tropism	NN
cord-017363-dmb42kna	15	35	towards	towards	IN
cord-017363-dmb42kna	15	36	respiratory	respiratory	JJ
cord-017363-dmb42kna	15	37	epithelial	epithelial	JJ
cord-017363-dmb42kna	15	38	cells	cell	NNS
cord-017363-dmb42kna	15	39	[	[	-LRB-
cord-017363-dmb42kna	15	40	3	3	CD
cord-017363-dmb42kna	15	41	]	]	-RRB-
cord-017363-dmb42kna	15	42	.	.	.
cord-017363-dmb42kna	16	1	PRCV	PRCV	NNP
cord-017363-dmb42kna	16	2	mostly	mostly	RB
cord-017363-dmb42kna	16	3	causes	cause	VBZ
cord-017363-dmb42kna	16	4	mild	mild	JJ
cord-017363-dmb42kna	16	5	or	or	CC
cord-017363-dmb42kna	16	6	subclinical	subclinical	JJ
cord-017363-dmb42kna	16	7	respiratory	respiratory	JJ
cord-017363-dmb42kna	16	8	disease	disease	NN
cord-017363-dmb42kna	16	9	.	.	.
cord-017363-dmb42kna	17	1	However	however	RB
cord-017363-dmb42kna	17	2	,	,	,
cord-017363-dmb42kna	17	3	in	in	IN
cord-017363-dmb42kna	17	4	some	some	DT
cord-017363-dmb42kna	17	5	PRCV	PRCV	NNP
cord-017363-dmb42kna	17	6	-	-	HYPH
cord-017363-dmb42kna	17	7	infected	infect	VBN
cord-017363-dmb42kna	17	8	pigs	pig	NNS
cord-017363-dmb42kna	17	9	,	,	,
cord-017363-dmb42kna	17	10	the	the	DT
cord-017363-dmb42kna	17	11	virus	virus	NN
cord-017363-dmb42kna	17	12	can	can	MD
cord-017363-dmb42kna	17	13	be	be	VB
cord-017363-dmb42kna	17	14	shed	shed	VBN
cord-017363-dmb42kna	17	15	in	in	IN
cord-017363-dmb42kna	17	16	the	the	DT
cord-017363-dmb42kna	17	17	feces	fece	NNS
cord-017363-dmb42kna	17	18	[	[	-LRB-
cord-017363-dmb42kna	17	19	4	4	CD
cord-017363-dmb42kna	17	20	]	]	-RRB-
cord-017363-dmb42kna	17	21	.	.	.
cord-017363-dmb42kna	18	1	Realtime	Realtime	NNP
cord-017363-dmb42kna	18	2	PCR	PCR	NNP
cord-017363-dmb42kna	18	3	-based	-base	VBN
cord-017363-dmb42kna	18	4	assays	assay	NNS
cord-017363-dmb42kna	18	5	are	be	VBP
cord-017363-dmb42kna	18	6	well	well	RB
cord-017363-dmb42kna	18	7	suited	suited	JJ
cord-017363-dmb42kna	18	8	for	for	IN
cord-017363-dmb42kna	18	9	rapid	rapid	JJ
cord-017363-dmb42kna	18	10	,	,	,
cord-017363-dmb42kna	18	11	specifi	specifi	NNP
cord-017363-dmb42kna	18	12	c	c	NN
cord-017363-dmb42kna	18	13	,	,	,
cord-017363-dmb42kna	18	14	and	and	CC
cord-017363-dmb42kna	18	15	sensitive	sensitive	JJ
cord-017363-dmb42kna	18	16	detection	detection	NN
cord-017363-dmb42kna	18	17	of	of	IN
cord-017363-dmb42kna	18	18	viruses	virus	NNS
cord-017363-dmb42kna	18	19	such	such	JJ
cord-017363-dmb42kna	18	20	as	as	IN
cord-017363-dmb42kna	18	21	TGEV	TGEV	NNP
cord-017363-dmb42kna	18	22	.	.	.
cord-017363-dmb42kna	19	1	The	the	DT
cord-017363-dmb42kna	19	2	real	real	JJ
cord-017363-dmb42kna	19	3	-	-	HYPH
cord-017363-dmb42kna	19	4	time	time	NN
cord-017363-dmb42kna	19	5	RT	RT	NNP
cord-017363-dmb42kna	19	6	-	-	HYPH
cord-017363-dmb42kna	19	7	PCR	PCR	NNP
cord-017363-dmb42kna	19	8	assay	assay	NN
cord-017363-dmb42kna	19	9	described	describe	VBN
cord-017363-dmb42kna	19	10	here	here	RB
cord-017363-dmb42kna	19	11	is	be	VBZ
cord-017363-dmb42kna	19	12	based	base	VBN
cord-017363-dmb42kna	19	13	on	on	IN
cord-017363-dmb42kna	19	14	amplifi	amplifi	NNP
cord-017363-dmb42kna	19	15	cation	cation	NN
cord-017363-dmb42kna	19	16	of	of	IN
cord-017363-dmb42kna	19	17	a	a	DT
cord-017363-dmb42kna	19	18	conserved	conserved	JJ
cord-017363-dmb42kna	19	19	region	region	NN
cord-017363-dmb42kna	19	20	of	of	IN
cord-017363-dmb42kna	19	21	the	the	DT
cord-017363-dmb42kna	19	22	spike	spike	NN
cord-017363-dmb42kna	19	23	protein	protein	NN
cord-017363-dmb42kna	19	24	gene	gene	NN
cord-017363-dmb42kna	19	25	of	of	IN
cord-017363-dmb42kna	19	26	TGEV	TGEV	NNP
cord-017363-dmb42kna	19	27	strains	strain	NNS
cord-017363-dmb42kna	19	28	and	and	CC
cord-017363-dmb42kna	19	29	detection	detection	NN
cord-017363-dmb42kna	19	30	of	of	IN
cord-017363-dmb42kna	19	31	the	the	DT
cord-017363-dmb42kna	19	32	amplifi	amplifi	NNP
cord-017363-dmb42kna	19	33	ed	ed	NNP
cord-017363-dmb42kna	19	34	products	product	NNS
cord-017363-dmb42kna	19	35	using	use	VBG
cord-017363-dmb42kna	19	36	a	a	DT
cord-017363-dmb42kna	19	37	TaqMan	TaqMan	NNP
cord-017363-dmb42kna	19	38	probe	probe	NN
cord-017363-dmb42kna	19	39	[	[	-LRB-
cord-017363-dmb42kna	19	40	5	5	CD
cord-017363-dmb42kna	19	41	]	]	-RRB-
cord-017363-dmb42kna	19	42	.	.	.
cord-017363-dmb42kna	20	1	The	the	DT
cord-017363-dmb42kna	20	2	assay	assay	NN
cord-017363-dmb42kna	20	3	,	,	,
cord-017363-dmb42kna	20	4	along	along	IN
cord-017363-dmb42kna	20	5	with	with	IN
cord-017363-dmb42kna	20	6	the	the	DT
cord-017363-dmb42kna	20	7	RNA	RNA	NNP
cord-017363-dmb42kna	20	8	extraction	extraction	NN
cord-017363-dmb42kna	20	9	method	method	NN
cord-017363-dmb42kna	20	10	described	describe	VBN
cord-017363-dmb42kna	20	11	here	here	RB
cord-017363-dmb42kna	20	12	,	,	,
cord-017363-dmb42kna	20	13	can	can	MD
cord-017363-dmb42kna	20	14	be	be	VB
cord-017363-dmb42kna	20	15	established	establish	VBN
cord-017363-dmb42kna	20	16	in	in	IN
cord-017363-dmb42kna	20	17	any	any	DT
cord-017363-dmb42kna	20	18	molecular	molecular	JJ
cord-017363-dmb42kna	20	19	diagnostic	diagnostic	JJ
cord-017363-dmb42kna	20	20	laboratory	laboratory	NN
cord-017363-dmb42kna	20	21	for	for	IN
cord-017363-dmb42kna	20	22	detection	detection	NN
cord-017363-dmb42kna	20	23	of	of	IN
cord-017363-dmb42kna	20	24	TGEV	TGEV	NNP
cord-017363-dmb42kna	20	25	in	in	IN
cord-017363-dmb42kna	20	26	pig	pig	NN
cord-017363-dmb42kna	20	27	fecal	fecal	NN
cord-017363-dmb42kna	20	28	samples	sample	NNS
cord-017363-dmb42kna	20	29	[	[	-LRB-
cord-017363-dmb42kna	20	30	6	6	CD
cord-017363-dmb42kna	20	31	-8	-8	NNPS
cord-017363-dmb42kna	20	32	]	]	-RRB-
cord-017363-dmb42kna	20	33	.	.	.
cord-017363-dmb42kna	21	1	Preventing	prevent	VBG
cord-017363-dmb42kna	21	2	contamination	contamination	NN
cord-017363-dmb42kna	21	3	of	of	IN
cord-017363-dmb42kna	21	4	samples	sample	NNS
cord-017363-dmb42kna	21	5	and	and	CC
cord-017363-dmb42kna	21	6	reagents	reagent	NNS
cord-017363-dmb42kna	21	7	with	with	IN
cord-017363-dmb42kna	21	8	nucleic	nucleic	JJ
cord-017363-dmb42kna	21	9	acids	acid	NNS
cord-017363-dmb42kna	21	10	and	and	CC
cord-017363-dmb42kna	21	11	nucleases	nuclease	NNS
cord-017363-dmb42kna	21	12	is	be	VBZ
cord-017363-dmb42kna	21	13	critical	critical	JJ
cord-017363-dmb42kna	21	14	to	to	IN
cord-017363-dmb42kna	21	15	obtaining	obtain	VBG
cord-017363-dmb42kna	21	16	accurate	accurate	JJ
cord-017363-dmb42kna	21	17	and	and	CC
cord-017363-dmb42kna	21	18	reproducible	reproducible	JJ
cord-017363-dmb42kna	21	19	results	result	NNS
cord-017363-dmb42kna	21	20	with	with	IN
cord-017363-dmb42kna	21	21	any	any	DT
cord-017363-dmb42kna	21	22	PCR	PCR	NNP
cord-017363-dmb42kna	21	23	-based	-base	VBN
cord-017363-dmb42kna	21	24	diagnostic	diagnostic	JJ
cord-017363-dmb42kna	21	25	assay	assay	NN
cord-017363-dmb42kna	21	26	.	.	.
cord-017363-dmb42kna	22	1	It	-PRON-	PRP
cord-017363-dmb42kna	22	2	is	be	VBZ
cord-017363-dmb42kna	22	3	recommended	recommend	VBN
cord-017363-dmb42kna	22	4	that	that	IN
cord-017363-dmb42kna	22	5	the	the	DT
cord-017363-dmb42kna	22	6	nucleic	nucleic	JJ
cord-017363-dmb42kna	22	7	acid	acid	NN
cord-017363-dmb42kna	22	8	extraction	extraction	NN
cord-017363-dmb42kna	22	9	,	,	,
cord-017363-dmb42kna	22	10	preparation	preparation	NN
cord-017363-dmb42kna	22	11	of	of	IN
cord-017363-dmb42kna	22	12	master	master	NN
cord-017363-dmb42kna	22	13	mix	mix	NN
cord-017363-dmb42kna	22	14	,	,	,
cord-017363-dmb42kna	22	15	and	and	CC
cord-017363-dmb42kna	22	16	real	real	JJ
cord-017363-dmb42kna	22	17	-	-	HYPH
cord-017363-dmb42kna	22	18	time	time	NN
cord-017363-dmb42kna	22	19	PCR	PCR	NNP
cord-017363-dmb42kna	22	20	amplifi	amplifi	NNP
cord-017363-dmb42kna	22	21	cation	cation	NN
cord-017363-dmb42kna	22	22	are	be	VBP
cord-017363-dmb42kna	22	23	performed	perform	VBN
cord-017363-dmb42kna	22	24	in	in	IN
cord-017363-dmb42kna	22	25	three	three	CD
cord-017363-dmb42kna	22	26	physically	physically	RB
cord-017363-dmb42kna	22	27	separated	separate	VBN
cord-017363-dmb42kna	22	28	areas	area	NNS
cord-017363-dmb42kna	22	29	.	.	.
cord-017363-dmb42kna	23	1	Each	each	DT
cord-017363-dmb42kna	23	2	of	of	IN
cord-017363-dmb42kna	23	3	these	these	DT
cord-017363-dmb42kna	23	4	areas	area	NNS
cord-017363-dmb42kna	23	5	should	should	MD
cord-017363-dmb42kna	23	6	have	have	VB
cord-017363-dmb42kna	23	7	separate	separate	JJ
cord-017363-dmb42kna	23	8	set	set	NN
cord-017363-dmb42kna	23	9	of	of	IN
cord-017363-dmb42kna	23	10	laboratory	laboratory	NN
cord-017363-dmb42kna	23	11	instruments	instrument	NNS
cord-017363-dmb42kna	23	12	and	and	CC
cord-017363-dmb42kna	23	13	supplies	supply	NNS
cord-017363-dmb42kna	23	14	that	that	WDT
cord-017363-dmb42kna	23	15	are	be	VBP
cord-017363-dmb42kna	23	16	to	to	TO
cord-017363-dmb42kna	23	17	be	be	VB
cord-017363-dmb42kna	23	18	used	use	VBN
cord-017363-dmb42kna	23	19	only	only	RB
cord-017363-dmb42kna	23	20	in	in	IN
cord-017363-dmb42kna	23	21	their	-PRON-	PRP$
cord-017363-dmb42kna	23	22	assigned	assign	VBN
cord-017363-dmb42kna	23	23	location	location	NN
cord-017363-dmb42kna	23	24	[	[	-LRB-
cord-017363-dmb42kna	23	25	9	9	CD
cord-017363-dmb42kna	23	26	]	]	-RRB-
cord-017363-dmb42kna	23	27	.	.	.
cord-017363-dmb42kna	24	1	Including	include	VBG
cord-017363-dmb42kna	24	2	a	a	DT
cord-017363-dmb42kna	24	3	positive	positive	JJ
cord-017363-dmb42kna	24	4	extraction	extraction	NN
cord-017363-dmb42kna	24	5	control	control	NN
cord-017363-dmb42kna	24	6	and	and	CC
cord-017363-dmb42kna	24	7	a	a	DT
cord-017363-dmb42kna	24	8	negative	negative	JJ
cord-017363-dmb42kna	24	9	extraction	extraction	NN
cord-017363-dmb42kna	24	10	control	control	NN
cord-017363-dmb42kna	24	11	along	along	IN
cord-017363-dmb42kna	24	12	with	with	IN
cord-017363-dmb42kna	24	13	each	each	DT
cord-017363-dmb42kna	24	14	batch	batch	NN
cord-017363-dmb42kna	24	15	of	of	IN
cord-017363-dmb42kna	24	16	clinical	clinical	JJ
cord-017363-dmb42kna	24	17	samples	sample	NNS
cord-017363-dmb42kna	24	18	is	be	VBZ
cord-017363-dmb42kna	24	19	recommended	recommend	VBN
cord-017363-dmb42kna	24	20	to	to	TO
cord-017363-dmb42kna	24	21	monitor	monitor	VB
cord-017363-dmb42kna	24	22	the	the	DT
cord-017363-dmb42kna	24	23	effi	effi	NNP
cord-017363-dmb42kna	24	24	ciency	ciency	NNP
cord-017363-dmb42kna	24	25	of	of	IN
cord-017363-dmb42kna	24	26	RNA	RNA	NNP
cord-017363-dmb42kna	24	27	extraction	extraction	NN
cord-017363-dmb42kna	24	28	and	and	CC
cord-017363-dmb42kna	24	29	potential	potential	JJ
cord-017363-dmb42kna	24	30	cross	cross	NN
cord-017363-dmb42kna	24	31	contamination	contamination	NN
cord-017363-dmb42kna	24	32	of	of	IN
cord-017363-dmb42kna	24	33	the	the	DT
cord-017363-dmb42kna	24	34	samples	sample	NNS
cord-017363-dmb42kna	24	35	[	[	-LRB-
cord-017363-dmb42kna	24	36	9	9	CD
cord-017363-dmb42kna	24	37	]	]	-RRB-
cord-017363-dmb42kna	24	38	.	.	.
cord-017363-dmb42kna	25	1	PCR	PCR	NNP
cord-017363-dmb42kna	25	2	inhibitors	inhibitor	NNS
cord-017363-dmb42kna	25	3	are	be	VBP
cord-017363-dmb42kna	25	4	often	often	RB
cord-017363-dmb42kna	25	5	co	co	VBN
cord-017363-dmb42kna	25	6	-	-	VBN
cord-017363-dmb42kna	25	7	extracted	extract	VBN
cord-017363-dmb42kna	25	8	with	with	IN
cord-017363-dmb42kna	25	9	nucleic	nucleic	JJ
cord-017363-dmb42kna	25	10	acids	acid	NNS
cord-017363-dmb42kna	25	11	from	from	IN
cord-017363-dmb42kna	25	12	fecal	fecal	JJ
cord-017363-dmb42kna	25	13	samples	sample	NNS
cord-017363-dmb42kna	25	14	.	.	.
cord-017363-dmb42kna	26	1	In	in	IN
cord-017363-dmb42kna	26	2	our	-PRON-	PRP$
cord-017363-dmb42kna	26	3	experience	experience	NN
cord-017363-dmb42kna	26	4	,	,	,
cord-017363-dmb42kna	26	5	RNA	RNA	NNP
cord-017363-dmb42kna	26	6	extracted	extract	VBD
cord-017363-dmb42kna	26	7	from	from	IN
cord-017363-dmb42kna	26	8	pig	pig	NN
cord-017363-dmb42kna	26	9	fecal	fecal	NN
cord-017363-dmb42kna	26	10	samples	sample	NNS
cord-017363-dmb42kna	26	11	using	use	VBG
cord-017363-dmb42kna	26	12	the	the	DT
cord-017363-dmb42kna	26	13	following	following	JJ
cord-017363-dmb42kna	26	14	method	method	NN
cord-017363-dmb42kna	26	15	is	be	VBZ
cord-017363-dmb42kna	26	16	suitable	suitable	JJ
cord-017363-dmb42kna	26	17	for	for	IN
cord-017363-dmb42kna	26	18	TGEV	TGEV	NNP
cord-017363-dmb42kna	26	19	detection	detection	NN
cord-017363-dmb42kna	26	20	using	use	VBG
cord-017363-dmb42kna	26	21	the	the	DT
cord-017363-dmb42kna	26	22	real	real	JJ
cord-017363-dmb42kna	26	23	-	-	HYPH
cord-017363-dmb42kna	26	24	time	time	NN
cord-017363-dmb42kna	26	25	RT	RT	NNP
cord-017363-dmb42kna	26	26	-	-	HYPH
cord-017363-dmb42kna	26	27	PCR	PCR	NNP
cord-017363-dmb42kna	26	28	assay	assay	NN
cord-017363-dmb42kna	26	29	.	.	.
cord-017363-dmb42kna	27	1	Other	other	JJ
cord-017363-dmb42kna	27	2	extraction	extraction	NN
cord-017363-dmb42kna	27	3	methods	method	NNS
cord-017363-dmb42kna	27	4	that	that	WDT
cord-017363-dmb42kna	27	5	produce	produce	VBP
cord-017363-dmb42kna	27	6	PCR	PCR	NNP
cord-017363-dmb42kna	27	7	inhibitor	inhibitor	NN
cord-017363-dmb42kna	27	8	-	-	HYPH
cord-017363-dmb42kna	27	9	free	free	JJ
cord-017363-dmb42kna	27	10	RNA	RNA	NNP
cord-017363-dmb42kna	27	11	can	can	MD
cord-017363-dmb42kna	27	12	also	also	RB
cord-017363-dmb42kna	27	13	be	be	VB
cord-017363-dmb42kna	27	14	used	use	VBN
cord-017363-dmb42kna	27	15	(	(	-LRB-
cord-017363-dmb42kna	27	16	see	see	VB
cord-017363-dmb42kna	27	17	Note	Note	NNP
cord-017363-dmb42kna	27	18	1	1	CD
cord-017363-dmb42kna	27	19	)	)	-RRB-
cord-017363-dmb42kna	27	20	.	.	.
cord-017363-dmb42kna	28	1	The	the	DT
cord-017363-dmb42kna	28	2	presence	presence	NN
cord-017363-dmb42kna	28	3	of	of	IN
cord-017363-dmb42kna	28	4	PCR	PCR	NNP
cord-017363-dmb42kna	28	5	inhibitors	inhibitor	NNS
cord-017363-dmb42kna	28	6	in	in	IN
cord-017363-dmb42kna	28	7	the	the	DT
cord-017363-dmb42kna	28	8	extracted	extract	VBN
cord-017363-dmb42kna	28	9	can	can	MD
cord-017363-dmb42kna	28	10	be	be	VB
cord-017363-dmb42kna	28	11	monitored	monitor	VBN
cord-017363-dmb42kna	28	12	by	by	IN
cord-017363-dmb42kna	28	13	using	use	VBG
cord-017363-dmb42kna	28	14	an	an	DT
cord-017363-dmb42kna	28	15	internal	internal	JJ
cord-017363-dmb42kna	28	16	control	control	NN
cord-017363-dmb42kna	28	17	(	(	-LRB-
cord-017363-dmb42kna	28	18	see	see	VB
cord-017363-dmb42kna	28	19	Note	Note	NNP
cord-017363-dmb42kna	28	20	2	2	CD
cord-017363-dmb42kna	28	21	)	)	-RRB-
cord-017363-dmb42kna	28	22	.	.	.
cord-017363-dmb42kna	29	1	1	1	LS
cord-017363-dmb42kna	29	2	.	.	.
cord-017363-dmb42kna	30	1	Prepare	prepare	VB
cord-017363-dmb42kna	30	2	a	a	DT
cord-017363-dmb42kna	30	3	20	20	CD
cord-017363-dmb42kna	30	4	%	%	NN
cord-017363-dmb42kna	30	5	(	(	-LRB-
cord-017363-dmb42kna	30	6	w	w	NN
cord-017363-dmb42kna	30	7	/	/	SYM
cord-017363-dmb42kna	30	8	v	v	NN
cord-017363-dmb42kna	30	9	)	)	-RRB-
cord-017363-dmb42kna	30	10	suspension	suspension	NN
cord-017363-dmb42kna	30	11	of	of	IN
cord-017363-dmb42kna	30	12	feces	fece	NNS
cord-017363-dmb42kna	30	13	in	in	IN
cord-017363-dmb42kna	30	14	DNase	DNase	NNP
cord-017363-dmb42kna	30	15	/	/	SYM
cord-017363-dmb42kna	30	16	RNasefree	RNasefree	NNP
cord-017363-dmb42kna	30	17	distilled	distilled	JJ
cord-017363-dmb42kna	30	18	water	water	NN
cord-017363-dmb42kna	30	19	.	.	.
cord-017363-dmb42kna	31	1	Feces	fece	NNS
cord-017363-dmb42kna	31	2	in	in	IN
cord-017363-dmb42kna	31	3	liquid	liquid	JJ
cord-017363-dmb42kna	31	4	form	form	NN
cord-017363-dmb42kna	31	5	can	can	MD
cord-017363-dmb42kna	31	6	be	be	VB
cord-017363-dmb42kna	31	7	used	use	VBN
cord-017363-dmb42kna	31	8	directly	directly	RB
cord-017363-dmb42kna	31	9	.	.	.
cord-017363-dmb42kna	32	1	2	2	LS
cord-017363-dmb42kna	32	2	.	.	.
cord-017363-dmb42kna	33	1	Transfer	transfer	VB
cord-017363-dmb42kna	33	2	250	250	CD
cord-017363-dmb42kna	33	3	μl	μl	NN
cord-017363-dmb42kna	33	4	of	of	IN
cord-017363-dmb42kna	33	5	the	the	DT
cord-017363-dmb42kna	33	6	fecal	fecal	JJ
cord-017363-dmb42kna	33	7	suspension	suspension	NN
cord-017363-dmb42kna	33	8	into	into	IN
cord-017363-dmb42kna	33	9	a	a	DT
cord-017363-dmb42kna	33	10	1.5	1.5	CD
cord-017363-dmb42kna	33	11	ml	ml	NN
cord-017363-dmb42kna	33	12	microcentrifuge	microcentrifuge	NN
cord-017363-dmb42kna	33	13	tube	tube	NN
cord-017363-dmb42kna	33	14	.	.	.
cord-017363-dmb42kna	34	1	3	3	LS
cord-017363-dmb42kna	34	2	.	.	.
cord-017363-dmb42kna	35	1	Add	add	VB
cord-017363-dmb42kna	35	2	750	750	CD
cord-017363-dmb42kna	35	3	μl	μl	NN
cord-017363-dmb42kna	35	4	of	of	IN
cord-017363-dmb42kna	35	5	TRIzol	TRIzol	NNP
cord-017363-dmb42kna	35	6	LS	LS	NNP
cord-017363-dmb42kna	35	7	reagent	reagent	NN
cord-017363-dmb42kna	35	8	,	,	,
cord-017363-dmb42kna	35	9	briefl	briefl	NNP
cord-017363-dmb42kna	35	10	y	y	NNP
cord-017363-dmb42kna	35	11	vortex	vortex	VBP
cord-017363-dmb42kna	35	12	the	the	DT
cord-017363-dmb42kna	35	13	tube	tube	NN
cord-017363-dmb42kna	35	14	for	for	IN
cord-017363-dmb42kna	35	15	10	10	CD
cord-017363-dmb42kna	35	16	s	s	NNS
cord-017363-dmb42kna	35	17	,	,	,
cord-017363-dmb42kna	35	18	and	and	CC
cord-017363-dmb42kna	35	19	incubate	incubate	VB
cord-017363-dmb42kna	35	20	at	at	IN
cord-017363-dmb42kna	35	21	room	room	NN
cord-017363-dmb42kna	35	22	temperature	temperature	NN
cord-017363-dmb42kna	35	23	for	for	IN
cord-017363-dmb42kna	35	24	5	5	CD
cord-017363-dmb42kna	35	25	min	min	NN
cord-017363-dmb42kna	35	26	.	.	.
cord-017363-dmb42kna	36	1	4	4	LS
cord-017363-dmb42kna	36	2	.	.	.
cord-017363-dmb42kna	37	1	Add	add	VB
cord-017363-dmb42kna	37	2	200	200	CD
cord-017363-dmb42kna	37	3	μl	μl	NN
cord-017363-dmb42kna	37	4	of	of	IN
cord-017363-dmb42kna	37	5	chloroform	chloroform	NN
cord-017363-dmb42kna	37	6	to	to	IN
cord-017363-dmb42kna	37	7	the	the	DT
cord-017363-dmb42kna	37	8	tube	tube	NN
cord-017363-dmb42kna	37	9	.	.	.
cord-017363-dmb42kna	38	1	Vortex	vortex	VB
cord-017363-dmb42kna	38	2	the	the	DT
cord-017363-dmb42kna	38	3	tube	tube	NN
cord-017363-dmb42kna	38	4	for	for	IN
cord-017363-dmb42kna	38	5	5	5	CD
cord-017363-dmb42kna	38	6	s	s	NNS
cord-017363-dmb42kna	38	7	and	and	CC
cord-017363-dmb42kna	38	8	incubate	incubate	VB
cord-017363-dmb42kna	38	9	at	at	IN
cord-017363-dmb42kna	38	10	room	room	NN
cord-017363-dmb42kna	38	11	temperature	temperature	NN
cord-017363-dmb42kna	38	12	for	for	IN
cord-017363-dmb42kna	38	13	3	3	CD
cord-017363-dmb42kna	38	14	min	min	NN
cord-017363-dmb42kna	38	15	.	.	NNP
cord-017363-dmb42kna	38	16	5	5	CD
cord-017363-dmb42kna	38	17	.	.	.
cord-017363-dmb42kna	39	1	Centrifuge	centrifuge	VB
cord-017363-dmb42kna	39	2	the	the	DT
cord-017363-dmb42kna	39	3	tube	tube	NN
cord-017363-dmb42kna	39	4	at	at	IN
cord-017363-dmb42kna	39	5	12,000	12,000	CD
cord-017363-dmb42kna	39	6	×	×	NN
cord-017363-dmb42kna	39	7	g	g	NN
cord-017363-dmb42kna	39	8	for	for	IN
cord-017363-dmb42kna	39	9	10	10	CD
cord-017363-dmb42kna	39	10	min	min	NN
cord-017363-dmb42kna	39	11	.	.	.
cord-017363-dmb42kna	40	1	11	11	CD
cord-017363-dmb42kna	40	2	.	.	.
cord-017363-dmb42kna	41	1	Repeat	repeat	VB
cord-017363-dmb42kna	41	2	step	step	NN
cord-017363-dmb42kna	41	3	10	10	CD
cord-017363-dmb42kna	41	4	for	for	IN
cord-017363-dmb42kna	41	5	a	a	DT
cord-017363-dmb42kna	41	6	second	second	JJ
cord-017363-dmb42kna	41	7	wash	wash	NN
cord-017363-dmb42kna	41	8	of	of	IN
cord-017363-dmb42kna	41	9	the	the	DT
cord-017363-dmb42kna	41	10	spin	spin	NN
cord-017363-dmb42kna	41	11	column	column	NN
cord-017363-dmb42kna	41	12	with	with	IN
cord-017363-dmb42kna	41	13	RPE	RPE	NNP
cord-017363-dmb42kna	41	14	buffer	buffer	NN
cord-017363-dmb42kna	41	15	.	.	.
cord-017363-dmb42kna	42	1	12	12	CD
cord-017363-dmb42kna	42	2	.	.	.
cord-017363-dmb42kna	43	1	Place	place	VB
cord-017363-dmb42kna	43	2	the	the	DT
cord-017363-dmb42kna	43	3	spin	spin	NN
cord-017363-dmb42kna	43	4	column	column	NN
cord-017363-dmb42kna	43	5	into	into	IN
cord-017363-dmb42kna	43	6	a	a	DT
cord-017363-dmb42kna	43	7	new	new	JJ
cord-017363-dmb42kna	43	8	collection	collection	NN
cord-017363-dmb42kna	43	9	tube	tube	NN
cord-017363-dmb42kna	43	10	and	and	CC
cord-017363-dmb42kna	43	11	centrifuge	centrifuge	NN
cord-017363-dmb42kna	43	12	at	at	IN
cord-017363-dmb42kna	43	13	10,000	10,000	CD
cord-017363-dmb42kna	43	14	×	×	NN
cord-017363-dmb42kna	43	15	g	g	NN
cord-017363-dmb42kna	43	16	for	for	IN
cord-017363-dmb42kna	43	17	1	1	CD
cord-017363-dmb42kna	43	18	min	min	NN
cord-017363-dmb42kna	43	19	to	to	TO
cord-017363-dmb42kna	43	20	dry	dry	VB
cord-017363-dmb42kna	43	21	the	the	DT
cord-017363-dmb42kna	43	22	membrane	membrane	NN
cord-017363-dmb42kna	43	23	of	of	IN
cord-017363-dmb42kna	43	24	the	the	DT
cord-017363-dmb42kna	43	25	column	column	NN
cord-017363-dmb42kna	43	26	.	.	.
cord-017363-dmb42kna	44	1	13	13	CD
cord-017363-dmb42kna	44	2	.	.	.
cord-017363-dmb42kna	45	1	Place	place	VB
cord-017363-dmb42kna	45	2	the	the	DT
cord-017363-dmb42kna	45	3	spin	spin	NN
cord-017363-dmb42kna	45	4	column	column	NN
cord-017363-dmb42kna	45	5	in	in	IN
cord-017363-dmb42kna	45	6	a	a	DT
cord-017363-dmb42kna	45	7	1.5	1.5	CD
cord-017363-dmb42kna	45	8	ml	ml	NN
cord-017363-dmb42kna	45	9	microcentrifuge	microcentrifuge	NN
cord-017363-dmb42kna	45	10	tube	tube	NN
cord-017363-dmb42kna	45	11	and	and	CC
cord-017363-dmb42kna	45	12	add	add	VB
cord-017363-dmb42kna	45	13	30	30	CD
cord-017363-dmb42kna	45	14	μl	μl	NN
cord-017363-dmb42kna	45	15	of	of	IN
cord-017363-dmb42kna	45	16	DNase	DNase	NNP
cord-017363-dmb42kna	45	17	/	/	SYM
cord-017363-dmb42kna	45	18	RNase	rnase	NN
cord-017363-dmb42kna	45	19	-	-	HYPH
cord-017363-dmb42kna	45	20	free	free	JJ
cord-017363-dmb42kna	45	21	water	water	NN
cord-017363-dmb42kna	45	22	to	to	IN
cord-017363-dmb42kna	45	23	the	the	DT
cord-017363-dmb42kna	45	24	column	column	NN
cord-017363-dmb42kna	45	25	.	.	.
cord-017363-dmb42kna	46	1	Incubate	incubate	VB
cord-017363-dmb42kna	46	2	at	at	IN
cord-017363-dmb42kna	46	3	room	room	NN
cord-017363-dmb42kna	46	4	temperature	temperature	NN
cord-017363-dmb42kna	46	5	for	for	IN
cord-017363-dmb42kna	46	6	1	1	CD
cord-017363-dmb42kna	46	7	min	min	NN
cord-017363-dmb42kna	46	8	.	.	.
cord-017363-dmb42kna	47	1	14	14	CD
cord-017363-dmb42kna	47	2	.	.	.
cord-017363-dmb42kna	48	1	Centrifuge	centrifuge	NN
cord-017363-dmb42kna	48	2	at	at	IN
cord-017363-dmb42kna	48	3	10,000	10,000	CD
cord-017363-dmb42kna	48	4	×	×	NN
cord-017363-dmb42kna	48	5	g	g	NN
cord-017363-dmb42kna	48	6	for	for	IN
cord-017363-dmb42kna	48	7	1	1	CD
cord-017363-dmb42kna	48	8	min	min	NN
cord-017363-dmb42kna	48	9	.	.	NNP
cord-017363-dmb42kna	48	10	15	15	CD
cord-017363-dmb42kna	48	11	.	.	.
cord-017363-dmb42kna	49	1	Discard	discard	VB
cord-017363-dmb42kna	49	2	the	the	DT
cord-017363-dmb42kna	49	3	spin	spin	NN
cord-017363-dmb42kna	49	4	column	column	NN
cord-017363-dmb42kna	49	5	and	and	CC
cord-017363-dmb42kna	49	6	store	store	VB
cord-017363-dmb42kna	49	7	the	the	DT
cord-017363-dmb42kna	49	8	eluted	elute	VBN
cord-017363-dmb42kna	49	9	RNA	RNA	NNP
cord-017363-dmb42kna	49	10	at	at	IN
cord-017363-dmb42kna	49	11	4	4	CD
cord-017363-dmb42kna	49	12	°	°	NN
cord-017363-dmb42kna	49	13	C	c	NN
cord-017363-dmb42kna	49	14	if	if	IN
cord-017363-dmb42kna	49	15	it	-PRON-	PRP
cord-017363-dmb42kna	49	16	is	be	VBZ
cord-017363-dmb42kna	49	17	used	use	VBN
cord-017363-dmb42kna	49	18	in	in	IN
cord-017363-dmb42kna	49	19	the	the	DT
cord-017363-dmb42kna	49	20	real	real	JJ
cord-017363-dmb42kna	49	21	-	-	HYPH
cord-017363-dmb42kna	49	22	time	time	NN
cord-017363-dmb42kna	49	23	RT	RT	NNP
cord-017363-dmb42kna	49	24	-	-	HYPH
cord-017363-dmb42kna	49	25	PCR	PCR	NNP
cord-017363-dmb42kna	49	26	assay	assay	NN
cord-017363-dmb42kna	49	27	within	within	IN
cord-017363-dmb42kna	49	28	12	12	CD
cord-017363-dmb42kna	49	29	h	h	NN
cord-017363-dmb42kna	49	30	or	or	CC
cord-017363-dmb42kna	49	31	at	at	IN
cord-017363-dmb42kna	49	32	−20	−20	NNP
cord-017363-dmb42kna	49	33	°	°	NN
cord-017363-dmb42kna	49	34	C	C	NNP
cord-017363-dmb42kna	49	35	if	if	IN
cord-017363-dmb42kna	49	36	it	-PRON-	PRP
cord-017363-dmb42kna	49	37	is	be	VBZ
cord-017363-dmb42kna	49	38	used	use	VBN
cord-017363-dmb42kna	49	39	after	after	IN
cord-017363-dmb42kna	49	40	12	12	CD
cord-017363-dmb42kna	49	41	h	h	NN
cord-017363-dmb42kna	49	42	(	(	-LRB-
cord-017363-dmb42kna	49	43	see	see	VB
cord-017363-dmb42kna	50	1	Note	note	NN
cord-017363-dmb42kna	50	2	4	4	CD
cord-017363-dmb42kna	50	3	)	)	-RRB-
cord-017363-dmb42kna	50	4	.	.	.
cord-017363-dmb42kna	51	1	1	1	LS
cord-017363-dmb42kna	51	2	.	.	.
cord-017363-dmb42kna	52	1	Turn	turn	VB
cord-017363-dmb42kna	52	2	on	on	IN
cord-017363-dmb42kna	52	3	the	the	DT
cord-017363-dmb42kna	52	4	real	real	JJ
cord-017363-dmb42kna	52	5	-	-	HYPH
cord-017363-dmb42kna	52	6	time	time	NN
cord-017363-dmb42kna	52	7	PCR	PCR	NNP
cord-017363-dmb42kna	52	8	machine	machine	NN
cord-017363-dmb42kna	52	9	.	.	.
cord-017363-dmb42kna	53	1	Follow	follow	VB
cord-017363-dmb42kna	53	2	the	the	DT
cord-017363-dmb42kna	53	3	software	software	NN
cord-017363-dmb42kna	53	4	directions	direction	NNS
cord-017363-dmb42kna	53	5	of	of	IN
cord-017363-dmb42kna	53	6	the	the	DT
cord-017363-dmb42kna	53	7	machine	machine	NN
cord-017363-dmb42kna	53	8	manufacturer	manufacturer	NN
cord-017363-dmb42kna	53	9	to	to	IN
cord-017363-dmb42kna	53	10	confi	confi	NNP
cord-017363-dmb42kna	53	11	rm	rm	NNP
cord-017363-dmb42kna	53	12	that	that	IN
cord-017363-dmb42kna	53	13	the	the	DT
cord-017363-dmb42kna	53	14	FAM	FAM	NNP
cord-017363-dmb42kna	53	15	signal	signal	NN
cord-017363-dmb42kna	53	16	data	datum	NNS
cord-017363-dmb42kna	53	17	will	will	MD
cord-017363-dmb42kna	53	18	be	be	VB
cord-017363-dmb42kna	53	19	gathered	gather	VBN
cord-017363-dmb42kna	53	20	during	during	IN
cord-017363-dmb42kna	53	21	the	the	DT
cord-017363-dmb42kna	53	22	amplifi	amplifi	NNP
cord-017363-dmb42kna	53	23	cation	cation	NN
cord-017363-dmb42kna	53	24	.	.	.
cord-017363-dmb42kna	54	1	Program	program	VB
cord-017363-dmb42kna	54	2	the	the	DT
cord-017363-dmb42kna	54	3	thermal	thermal	JJ
cord-017363-dmb42kna	54	4	cycle	cycle	NN
cord-017363-dmb42kna	54	5	and	and	CC
cord-017363-dmb42kna	54	6	data	datum	NNS
cord-017363-dmb42kna	54	7	collection	collection	NN
cord-017363-dmb42kna	54	8	conditions	condition	NNS
cord-017363-dmb42kna	54	9	according	accord	VBG
cord-017363-dmb42kna	54	10	to	to	IN
cord-017363-dmb42kna	54	11	Table	Table	NNP
cord-017363-dmb42kna	54	12	1	1	CD
cord-017363-dmb42kna	54	13	.	.	CD
cord-017363-dmb42kna	54	14	2	2	CD
cord-017363-dmb42kna	54	15	.	.	.
cord-017363-dmb42kna	55	1	Prepare	prepare	VB
cord-017363-dmb42kna	55	2	a	a	DT
cord-017363-dmb42kna	55	3	master	master	NN
cord-017363-dmb42kna	55	4	mix	mix	VB
cord-017363-dmb42kna	55	5	suffi	suffi	NNP
cord-017363-dmb42kna	55	6	cient	cient	NNP
cord-017363-dmb42kna	55	7	for	for	IN
cord-017363-dmb42kna	55	8	the	the	DT
cord-017363-dmb42kna	55	9	intended	intend	VBN
cord-017363-dmb42kna	55	10	number	number	NN
cord-017363-dmb42kna	55	11	of	of	IN
cord-017363-dmb42kna	55	12	samples	sample	NNS
cord-017363-dmb42kna	55	13	in	in	IN
cord-017363-dmb42kna	55	14	a	a	DT
cord-017363-dmb42kna	55	15	sterile	sterile	JJ
cord-017363-dmb42kna	55	16	1.5	1.5	CD
cord-017363-dmb42kna	55	17	ml	ml	NN
cord-017363-dmb42kna	55	18	microcentrifuge	microcentrifuge	NN
cord-017363-dmb42kna	55	19	tube	tube	NN
cord-017363-dmb42kna	55	20	according	accord	VBG
cord-017363-dmb42kna	55	21	to	to	IN
cord-017363-dmb42kna	55	22	Table	table	NN
cord-017363-dmb42kna	55	23	2	2	CD
cord-017363-dmb42kna	55	24	.	.	.
cord-017363-dmb42kna	56	1	Add	add	VB
cord-017363-dmb42kna	56	2	at	at	IN
cord-017363-dmb42kna	56	3	least	least	JJS
cord-017363-dmb42kna	56	4	a	a	DT
cord-017363-dmb42kna	56	5	no	no	DT
cord-017363-dmb42kna	56	6	-	-	HYPH
cord-017363-dmb42kna	56	7	template	template	NN
cord-017363-dmb42kna	56	8	control	control	NN
cord-017363-dmb42kna	56	9	(	(	-LRB-
cord-017363-dmb42kna	56	10	NTC	NTC	NNP
cord-017363-dmb42kna	56	11	)	)	-RRB-
cord-017363-dmb42kna	56	12	and	and	CC
cord-017363-dmb42kna	56	13	a	a	DT
cord-017363-dmb42kna	56	14	positive	positive	JJ
cord-017363-dmb42kna	56	15	amplifi	amplifi	NNP
cord-017363-dmb42kna	56	16	cation	cation	NN
cord-017363-dmb42kna	56	17	control	control	NN
cord-017363-dmb42kna	56	18	(	(	-LRB-
cord-017363-dmb42kna	56	19	PAC	PAC	NNP
cord-017363-dmb42kna	56	20	)	)	-RRB-
cord-017363-dmb42kna	56	21	to	to	IN
cord-017363-dmb42kna	56	22	the	the	DT
cord-017363-dmb42kna	56	23	number	number	NN
cord-017363-dmb42kna	56	24	required	require	VBN
cord-017363-dmb42kna	56	25	reactions	reaction	NNS
cord-017363-dmb42kna	56	26	.	.	.
cord-017363-dmb42kna	57	1	3	3	LS
cord-017363-dmb42kna	57	2	.	.	.
cord-017363-dmb42kna	58	1	Close	close	VB
cord-017363-dmb42kna	58	2	the	the	DT
cord-017363-dmb42kna	58	3	cap	cap	NN
cord-017363-dmb42kna	58	4	of	of	IN
cord-017363-dmb42kna	58	5	the	the	DT
cord-017363-dmb42kna	58	6	microcentrifuge	microcentrifuge	NN
cord-017363-dmb42kna	58	7	tube	tube	NN
cord-017363-dmb42kna	58	8	.	.	.
cord-017363-dmb42kna	59	1	Vortex	vortex	NN
cord-017363-dmb42kna	59	2	and	and	CC
cord-017363-dmb42kna	59	3	centrifuge	centrifuge	VB
cord-017363-dmb42kna	59	4	the	the	DT
cord-017363-dmb42kna	59	5	tube	tube	NN
cord-017363-dmb42kna	59	6	briefl	briefl	NNP
cord-017363-dmb42kna	60	1	y.	y.	NN
cord-017363-dmb42kna	60	2	4	4	CD
cord-017363-dmb42kna	60	3	.	.	.
cord-017363-dmb42kna	61	1	Aliquot	Aliquot	NNP
cord-017363-dmb42kna	61	2	20	20	CD
cord-017363-dmb42kna	61	3	μl	μl	NN
cord-017363-dmb42kna	61	4	of	of	IN
cord-017363-dmb42kna	61	5	the	the	DT
cord-017363-dmb42kna	61	6	master	master	NN
cord-017363-dmb42kna	61	7	mix	mix	NN
cord-017363-dmb42kna	61	8	into	into	IN
cord-017363-dmb42kna	61	9	each	each	DT
cord-017363-dmb42kna	61	10	PCR	PCR	NNP
cord-017363-dmb42kna	61	11	reaction	reaction	NN
cord-017363-dmb42kna	61	12	tube	tube	NN
cord-017363-dmb42kna	61	13	.	.	.
cord-017363-dmb42kna	62	1	Table	table	NN
cord-017363-dmb42kna	62	2	1	1	CD
cord-017363-dmb42kna	62	3	Thermal	thermal	JJ
cord-017363-dmb42kna	62	4	cycling	cycling	NN
cord-017363-dmb42kna	62	5	conditions	condition	NNS
cord-017363-dmb42kna	62	6	Step	step	VBP
cord-017363-dmb42kna	62	7	Temperature	temperature	NN
cord-017363-dmb42kna	62	8	(	(	-LRB-
cord-017363-dmb42kna	62	9	°	°	NN
cord-017363-dmb42kna	62	10	C	c	NN
cord-017363-dmb42kna	62	11	)	)	-RRB-
cord-017363-dmb42kna	63	1	Time	Time	NNP
cord-017363-dmb42kna	63	2	 	 	_SP
cord-017363-dmb42kna	64	1	DNase	DNase	NNP
cord-017363-dmb42kna	64	2	/	/	SYM
cord-017363-dmb42kna	64	3	RNase	rnase	NN
cord-017363-dmb42kna	64	4	-	-	HYPH
cord-017363-dmb42kna	64	5	free	free	JJ
cord-017363-dmb42kna	64	6	distilled	distilled	JJ
cord-017363-dmb42kna	64	7	water	water	NN
cord-017363-dmb42kna	64	8	Feces	fece	NNS
cord-017363-dmb42kna	64	9	samples	sample	NNS
cord-017363-dmb42kna	64	10	from	from	IN
cord-017363-dmb42kna	64	11	suspected	suspect	VBN
cord-017363-dmb42kna	64	12	pigs	pig	NNS
cord-017363-dmb42kna	64	13	RNasin	RNasin	NNP
cord-017363-dmb42kna	64	14	Ribonuclease	Ribonuclease	NNP
cord-017363-dmb42kna	64	15	Inhibitor	Inhibitor	NNP
cord-017363-dmb42kna	64	16	(	(	-LRB-
cord-017363-dmb42kna	64	17	Promega	Promega	NNP
cord-017363-dmb42kna	64	18	)	)	-RRB-
cord-017363-dmb42kna	64	19	or	or	CC
cord-017363-dmb42kna	64	20	equivalent	equivalent	JJ
cord-017363-dmb42kna	64	21	mM	mm	CD
cord-017363-dmb42kna	64	22	MgCl	MgCl	NNP
cord-017363-dmb42kna	64	23	2	2	CD
cord-017363-dmb42kna	64	24	solution	solution	NN
cord-017363-dmb42kna	64	25	for	for	IN
cord-017363-dmb42kna	64	26	PCR	PCR	NNP
cord-017363-dmb42kna	64	27	(	(	-LRB-
cord-017363-dmb42kna	64	28	Sigma	Sigma	NNP
cord-017363-dmb42kna	64	29	-	-	HYPH
cord-017363-dmb42kna	64	30	Aldrich	Aldrich	NNP
cord-017363-dmb42kna	64	31	)	)	-RRB-
cord-017363-dmb42kna	64	32	or	or	CC
cord-017363-dmb42kna	64	33	equivalent	equivalent	JJ
cord-017363-dmb42kna	64	34	DNase	DNase	NNP
cord-017363-dmb42kna	64	35	/	/	SYM
cord-017363-dmb42kna	64	36	RNase	rnase	NN
cord-017363-dmb42kna	64	37	-	-	HYPH
cord-017363-dmb42kna	64	38	free	free	JJ
cord-017363-dmb42kna	64	39	distilled	distilled	JJ
cord-017363-dmb42kna	64	40	water	water	NN
cord-017363-dmb42kna	65	1	μM	μM	NNP
cord-017363-dmb42kna	66	1	Forward	forward	JJ
cord-017363-dmb42kna	66	2	primer	primer	NN
cord-017363-dmb42kna	66	3	CCA(BHQ1)-3′(FAM	CCA(BHQ1)-3′(FAM	NNP
cord-017363-dmb42kna	66	4	,	,	,
cord-017363-dmb42kna	66	5	6-carboxyfl	6-carboxyfl	CD
cord-017363-dmb42kna	66	6	uorescein	uorescein	NN
cord-017363-dmb42kna	66	7	;	;	:
cord-017363-dmb42kna	66	8	BHQ1	BHQ1	NNP
cord-017363-dmb42kna	66	9	,	,	,
cord-017363-dmb42kna	66	10	black	black	JJ
cord-017363-dmb42kna	66	11	hole	hole	NN
cord-017363-dmb42kna	66	12	quencher	quencher	NN
cord-017363-dmb42kna	66	13	1	1	CD
cord-017363-dmb42kna	66	14	;	;	:
cord-017363-dmb42kna	67	1	Eurofi	Eurofi	NNP
cord-017363-dmb42kna	67	2	ns	ns	IN
cord-017363-dmb42kna	67	3	MWG	MWG	NNP
cord-017363-dmb42kna	67	4	Operon	Operon	NNP
cord-017363-dmb42kna	67	5	)	)	-RRB-
cord-017363-dmb42kna	68	1	Real	real	JJ
cord-017363-dmb42kna	68	2	-	-	HYPH
cord-017363-dmb42kna	68	3	time	time	NN
cord-017363-dmb42kna	68	4	PCR	PCR	NNP
cord-017363-dmb42kna	68	5	machine	machine	NN
cord-017363-dmb42kna	68	6	,	,	,
cord-017363-dmb42kna	68	7	such	such	JJ
cord-017363-dmb42kna	68	8	as	as	IN
cord-017363-dmb42kna	68	9	Smart	Smart	NNP
cord-017363-dmb42kna	68	10	Cycler	Cycler	NNP
cord-017363-dmb42kna	68	11	II	II	NNP
cord-017363-dmb42kna	68	12	(	(	-LRB-
cord-017363-dmb42kna	68	13	Cepheid	Cepheid	NNP
cord-017363-dmb42kna	68	14	)	)	-RRB-
cord-017363-dmb42kna	68	15	,	,	.
cord-017363-dmb42kna	69	1	7300	7300	CD
cord-017363-dmb42kna	69	2	Real	real	JJ
cord-017363-dmb42kna	69	3	-	-	HYPH
cord-017363-dmb42kna	69	4	Time	time	NN
cord-017363-dmb42kna	69	5	PCR	pcr	NN
cord-017363-dmb42kna	69	6	System	system	NN
cord-017363-dmb42kna	69	7	(	(	-LRB-
cord-017363-dmb42kna	69	8	Applied	Applied	NNP
cord-017363-dmb42kna	69	9	Biosystems	Biosystems	NNP
cord-017363-dmb42kna	69	10	)	)	-RRB-
cord-017363-dmb42kna	69	11	,	,	,
cord-017363-dmb42kna	69	12	or	or	CC
cord-017363-dmb42kna	69	13	equivalent	equivalent	JJ
cord-017363-dmb42kna	69	14	PCR	PCR	NNP
cord-017363-dmb42kna	69	15	reaction	reaction	NN
cord-017363-dmb42kna	69	16	tubes	tube	NNS
cord-017363-dmb42kna	69	17	suitable	suitable	JJ
cord-017363-dmb42kna	69	18	for	for	IN
cord-017363-dmb42kna	69	19	the	the	DT
cord-017363-dmb42kna	69	20	real	real	JJ
cord-017363-dmb42kna	69	21	-	-	HYPH
cord-017363-dmb42kna	69	22	time	time	NN
cord-017363-dmb42kna	69	23	PCR	PCR	NNP
cord-017363-dmb42kna	69	24	machine	machine	NN
cord-017363-dmb42kna	69	25	platform	platform	NN
cord-017363-dmb42kna	69	26	Transmissible	transmissible	JJ
cord-017363-dmb42kna	69	27	gastroenteritis	gastroenteritis	NN
cord-017363-dmb42kna	69	28	Immunity	immunity	NN
cord-017363-dmb42kna	69	29	to	to	IN
cord-017363-dmb42kna	69	30	transmissible	transmissible	JJ
cord-017363-dmb42kna	69	31	gastroenteritis	gastroenteritis	NN
cord-017363-dmb42kna	69	32	virus	virus	NN
cord-017363-dmb42kna	69	33	and	and	CC
cord-017363-dmb42kna	69	34	porcine	porcine	JJ
cord-017363-dmb42kna	69	35	respiratory	respiratory	JJ
cord-017363-dmb42kna	69	36	coronavirus	coronavirus	NN
cord-017363-dmb42kna	69	37	infections	infection	NNS
cord-017363-dmb42kna	69	38	in	in	IN
cord-017363-dmb42kna	69	39	swine	swine	NN
cord-017363-dmb42kna	69	40	Porcine	porcine	JJ
cord-017363-dmb42kna	69	41	respiratory	respiratory	JJ
cord-017363-dmb42kna	69	42	coronavirus	coronavirus	NN
cord-017363-dmb42kna	69	43	differs	differ	VBZ
cord-017363-dmb42kna	69	44	from	from	IN
cord-017363-dmb42kna	69	45	transmissible	transmissible	JJ
cord-017363-dmb42kna	69	46	gastroenteritis	gastroenteritis	NN
cord-017363-dmb42kna	69	47	virus	virus	NN
cord-017363-dmb42kna	69	48	by	by	IN
cord-017363-dmb42kna	69	49	a	a	DT
cord-017363-dmb42kna	69	50	few	few	JJ
cord-017363-dmb42kna	69	51	genomic	genomic	JJ
cord-017363-dmb42kna	69	52	deletions	deletion	NNS
cord-017363-dmb42kna	69	53	Respiratory	respiratory	JJ
cord-017363-dmb42kna	69	54	and	and	CC
cord-017363-dmb42kna	69	55	fecal	fecal	JJ
cord-017363-dmb42kna	69	56	shedding	shedding	NN
cord-017363-dmb42kna	69	57	of	of	IN
cord-017363-dmb42kna	69	58	porcine	porcine	JJ
cord-017363-dmb42kna	69	59	respiratory	respiratory	JJ
cord-017363-dmb42kna	69	60	coronavirus	coronavirus	NN
cord-017363-dmb42kna	69	61	(	(	-LRB-
cord-017363-dmb42kna	69	62	PRCV	PRCV	NNP
cord-017363-dmb42kna	69	63	)	)	-RRB-
cord-017363-dmb42kna	70	1	in	in	IN
cord-017363-dmb42kna	70	2	sentinel	sentinel	NN
cord-017363-dmb42kna	70	3	weaned	wean	VBD
cord-017363-dmb42kna	70	4	pigs	pig	NNS
cord-017363-dmb42kna	70	5	and	and	CC
cord-017363-dmb42kna	70	6	sequence	sequence	NN
cord-017363-dmb42kna	70	7	of	of	IN
cord-017363-dmb42kna	70	8	the	the	DT
cord-017363-dmb42kna	70	9	partial	partial	JJ
cord-017363-dmb42kna	70	10	S	s	NN
cord-017363-dmb42kna	70	11	-	-	HYPH
cord-017363-dmb42kna	70	12	gene	gene	NN
cord-017363-dmb42kna	70	13	of	of	IN
cord-017363-dmb42kna	70	14	the	the	DT
cord-017363-dmb42kna	70	15	PRCV	PRCV	NNP
cord-017363-dmb42kna	70	16	isolates	isolate	VBZ
cord-017363-dmb42kna	71	1	A	a	DT
cord-017363-dmb42kna	71	2	real	real	JJ
cord-017363-dmb42kna	71	3	-	-	HYPH
cord-017363-dmb42kna	71	4	time	time	NN
cord-017363-dmb42kna	71	5	TaqMan	TaqMan	NNP
cord-017363-dmb42kna	71	6	®	®	HYPH
cord-017363-dmb42kna	71	7	RT	RT	NNP
cord-017363-dmb42kna	71	8	-	-	HYPH
cord-017363-dmb42kna	71	9	PCR	PCR	NNP
cord-017363-dmb42kna	71	10	assay	assay	NN
cord-017363-dmb42kna	71	11	with	with	IN
cord-017363-dmb42kna	71	12	an	an	DT
cord-017363-dmb42kna	71	13	internal	internal	JJ
cord-017363-dmb42kna	71	14	amplifi	amplifi	NNP
cord-017363-dmb42kna	71	15	cation	cation	NN
cord-017363-dmb42kna	71	16	control	control	NN
cord-017363-dmb42kna	71	17	for	for	IN
cord-017363-dmb42kna	71	18	rapid	rapid	JJ
cord-017363-dmb42kna	71	19	detection	detection	NN
cord-017363-dmb42kna	71	20	of	of	IN
cord-017363-dmb42kna	71	21	transmissible	transmissible	JJ
cord-017363-dmb42kna	71	22	gastroenteritis	gastroenteritis	JJ
cord-017363-dmb42kna	71	23	virus	virus	NN
cord-017363-dmb42kna	71	24	in	in	IN
cord-017363-dmb42kna	71	25	swine	swine	NN
cord-017363-dmb42kna	71	26	fecal	fecal	JJ
cord-017363-dmb42kna	71	27	samples	sample	NNS
cord-017363-dmb42kna	72	1	Attempted	attempt	VBN
cord-017363-dmb42kna	72	2	experimental	experimental	JJ
cord-017363-dmb42kna	72	3	reproduction	reproduction	NN
cord-017363-dmb42kna	72	4	of	of	IN
cord-017363-dmb42kna	72	5	porcine	porcine	JJ
cord-017363-dmb42kna	72	6	periweaning	periweaning	NN
cord-017363-dmb42kna	72	7	-	-	HYPH
cord-017363-dmb42kna	72	8	failure	failure	NN
cord-017363-dmb42kna	72	9	-	-	HYPH
cord-017363-dmb42kna	72	10	to	to	TO
cord-017363-dmb42kna	72	11	-	-	HYPH
cord-017363-dmb42kna	72	12	thrive	thrive	VB
cord-017363-dmb42kna	72	13	syndrome	syndrome	NN
cord-017363-dmb42kna	72	14	using	use	VBG
cord-017363-dmb42kna	72	15	tissue	tissue	NN
cord-017363-dmb42kna	72	16	homogenates	homogenate	NNS
cord-017363-dmb42kna	73	1	The	the	DT
cord-017363-dmb42kna	73	2	fi	fi	NN
cord-017363-dmb42kna	73	3	rst	rst	NN
cord-017363-dmb42kna	73	4	case	case	NN
cord-017363-dmb42kna	73	5	of	of	IN
cord-017363-dmb42kna	73	6	porcine	porcine	JJ
cord-017363-dmb42kna	73	7	epidemic	epidemic	NN
cord-017363-dmb42kna	73	8	diarrhea	diarrhea	NN
cord-017363-dmb42kna	73	9	in	in	IN
cord-017363-dmb42kna	73	10	Canada	Canada	NNP
cord-017363-dmb42kna	74	1	A	a	DT
cord-017363-dmb42kna	74	2	novel	novel	JJ
cord-017363-dmb42kna	74	3	watery	watery	JJ
cord-017363-dmb42kna	74	4	diarrhea	diarrhea	NN
cord-017363-dmb42kna	74	5	caused	cause	VBN
cord-017363-dmb42kna	74	6	by	by	IN
cord-017363-dmb42kna	74	7	the	the	DT
cord-017363-dmb42kna	74	8	co	co	NN
cord-017363-dmb42kna	74	9	-	-	NN
cord-017363-dmb42kna	74	10	infection	infection	NN
cord-017363-dmb42kna	74	11	of	of	IN
cord-017363-dmb42kna	74	12	neonatal	neonatal	JJ
cord-017363-dmb42kna	74	13	piglets	piglet	NNS
cord-017363-dmb42kna	74	14	with	with	IN
cord-017363-dmb42kna	74	15	Clostridium	clostridium	NN
cord-017363-dmb42kna	74	16	perfringens	perfringens	NN
cord-017363-dmb42kna	74	17	type	type	NN
cord-017363-dmb42kna	74	18	A	a	NN
cord-017363-dmb42kna	74	19	and	and	CC
cord-017363-dmb42kna	74	20	Escherichia	escherichia	NN
cord-017363-dmb42kna	74	21	coli	coli	NNS
cord-017363-dmb42kna	74	22	(	(	-LRB-
cord-017363-dmb42kna	74	23	K88	K88	NNP
cord-017363-dmb42kna	74	24	,	,	,
cord-017363-dmb42kna	74	25	987P	987p	CD
cord-017363-dmb42kna	74	26	)	)	-RRB-
cord-017363-dmb42kna	75	1	Quality	quality	NN
cord-017363-dmb42kna	75	2	assurance	assurance	NN
cord-017363-dmb42kna	75	3	and	and	CC
cord-017363-dmb42kna	75	4	quality	quality	NN
cord-017363-dmb42kna	75	5	control	control	NN
cord-017363-dmb42kna	75	6	in	in	IN
cord-017363-dmb42kna	75	7	the	the	DT
cord-017363-dmb42kna	75	8	routine	routine	JJ
cord-017363-dmb42kna	75	9	molecular	molecular	JJ
cord-017363-dmb42kna	75	10	diagnostic	diagnostic	JJ
cord-017363-dmb42kna	75	11	laboratory	laboratory	NN
cord-017363-dmb42kna	75	12	for	for	IN
cord-017363-dmb42kna	75	13	infectious	infectious	JJ
cord-017363-dmb42kna	75	14	diseases	disease	NNS