id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-355988-4eldkteb	SAMPATH, RANGARAJAN	Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry	2007-04-23		.txt	text/plain	3430	168	41	Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI‐MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. Most of the new technologies being developed for detection of infectious agents incorporate a version of quantitative polymerase chain reaction (PCR), based upon the use of highly specific primers and probes and designed to selectively identify specific pathogenic organisms. The process uses electrospray ionization mass spectrometry (ESI-MS) and base composition analysis of PCR amplification products from highly conserved genomic regions to identify and determine the relative quantity of pathogenic bacteria, FIGURE 1. Importantly, the base composition data for some of the isolates represented new measurements from regions that have not been sequenced, showing the potential of this strategy to identify previously unknown or newly emerging viruses.	./cache/cord-355988-4eldkteb.txt	./txt/cord-355988-4eldkteb.txt