id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-354773-u86bdmvf	Suo, Tao	ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens	2020-06-07		.txt	text/plain	4490	242	57	To improve the diagnostic accuracy of nucleic acid detection of SARS-Cov-2 in low viral load samples using droplet digital PCR, we compared the dynamic range and the limit of detection (LoD) with a 95% repeatable probability between ddPCR and RT-PCR in laboratory, and tested the clinical samples from 77 patients by both ddPCR and RT-PCR for head to head comparison. Throat swab samples of each patient were firstly collected for official approved RT-PCR diagnosis in hospitals and blinding laboratory RT-PCR and ddPCR tests simultaneously with the same primers/probe sets approved by Chinese CDC. As shown in Figure 3 , throat swab samples of each suspected outpatient were firstly collected for laboratory RT-PCR, ddPCR tests and official approved RT-PCR diagnosis in hospitals simultaneously with the same primers/probe sets approved by Chinese CDC (Table 1) . In this study, two throat swab samples of each supposed convalescent were collected for laboratory RT-PCR, ddPCR and official approved RT-PCR tests simultaneously with the same primers/probe sets (Table 2) .	./cache/cord-354773-u86bdmvf.txt	./txt/cord-354773-u86bdmvf.txt