id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353810-mf753ae9	Tan, Cedric Chih Shen	A novel method for the capture-based purification of whole viral native RNA genomes	2019-04-08		.txt	text/plain	5929	352	54	This report also describes a successful application of capture-based purification as a direct RNA sequencing strategy that addresses certain limitations of current strategies in sequencing RNA viral genomes. Based on the mapping rates to human and DENV1 reference genomes for pre and post-capture groups, shown in Table 2 , purification factor was calculated to be 272-fold. The minimum coverage required for variant calling was, as described above, benchmarked to that of Illumina reads so that the effectiveness of our capture-based purification method could be more accurately evaluated based on the higher error read rates of direct RNA sequencing technology. Indeed, after comparison of the postcapture and concentrated post-capture sequencing runs (Table 2) , the 2.5-fold increase in the percentage of reads mapping to DENV1 suggests that scaling our method greatly improved the signal-to-noise ratio of this particular downstream RNA assay.	./cache/cord-353810-mf753ae9.txt	./txt/cord-353810-mf753ae9.txt