id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-353573-y9jro9gt	You, Huey-Ling	Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms	2017-03-27		.txt	text/plain	3452	203	52	BACKGROUND: Both respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) are important viral pathogens causing respiratory tract infection (RTI) in the pediatric population. RESULTS: Our one-step triplex qRT-PCR assay showed 100% sensitivity and specificity in testing RSV and hMPV in 86 known virus culture supernatants, with excellent linearity (R(2) > 0.99) and reliable reproducibility (CV lower than 1.04%). The aim of this study is to design and assess the diagnostic performance of clinical specimens for the simultaneous identification of RSV and hMPV by using one-step triplex qRT-PCR assay with TaqMan probes. Our one-step triplex qRT-PCR results showed that the viral load of the RSV or hMPV-positive samples from clinical specimens varied over a wide range, presenting threshold between Ct 21 and 44 (21 and 44 cycles). In this study, we established a rapid and internally controlled triplex qRT-PCR assay that can identify RSV and hMPV virus in one reaction mixtures.	./cache/cord-353573-y9jro9gt.txt	./txt/cord-353573-y9jro9gt.txt