id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-352554-hsbyznex	Lee, Yeon Joo	Quality of Ribonucleic Acid Extraction for Real-Time Reverse Transcription-PCR (rRT-PCR) of SARS-CoV-2: Importance of Internal Control Monitoring	2020-11-01		.txt	text/plain	1122	64	56	On 6th February 2020, Emergency Use Authorization (EUA) for COVID-19 testing was implemented in Korea, permitting rapid expansion of capacity in clinical and public health laboratories [5] . To evaluate the performance of the RNA extraction step, 31 and 35 samples were prepared using the Real-Prep-in-use and the new Real-Prep system, respectively, and all 66 eluates were submitted to the same run of rRT-PCR. As the experience of the Middle East Respiratory Syndrome outbreak of 2015 in Korea, an epidemic of emerging infectious diseases necessitates clinical laboratories to conduct new tests at a large scale in a short period of time with kits and equipment that have not been thoroughly validated [10] . Analytical and clinical validation of six commercial Middle East Respiratory Syndrome coronavirus RNA detection kits based on real-time reverse-transcription PCR Comparative evaluation of three homogenization methods for isolating Middle East Respiratory Syndrome coronavirus nucleic acids from sputum samples for real-time reverse transcription PCR	./cache/cord-352554-hsbyznex.txt	./txt/cord-352554-hsbyznex.txt