id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-351643-8ce807ub	Poon, LLM	Rapid detection of reassortment of pandemic H1N1/2009 influenza virus	2010-08-01		.txt	text/plain	2085	111	52	It should be noted that all of the PCR-positive viral segments fall into the sister group of pandemic H1N1 (see online Supplemental Fig. 2) , which demonstrates the feasibility of using these real-time RT-PCR assays to detect genes from contemporary TR (PB2, PB1, PA, HA, NP, and NS) and EA (NA and M) swine viruses (2 ) . In contrast, melting-curve signals of the HA gene derived from the TR-H1 swine viruses were found to be different from that of the pandemic H1N1/ 2009 virus (Fig. 1B The sequence similarity and diversity of influenza viruses were the major hurdles for the primer design of this study. By use of the 8 established real-time PCR assays we tested RNA from 2 randomly selected samples of the original swine swab specimens that contained the pandemic H1N1/2009 virus. It should be noted that none of these assays can detect viral genes derived from seasonal human influenza viruses.	./cache/cord-351643-8ce807ub.txt	./txt/cord-351643-8ce807ub.txt