id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-350890-ajxvjkmq	Hsieh, Yi-Fan	A real-time convective PCR machine in a capillary tube instrumented with a CCD-based fluorometer	2013-07-05		.txt	text/plain	4214	207	47	This research reports the design, analysis, integration, and test of a prototype of a real-time convective polymerase chain reaction (RT-cPCR) machine that uses a color charged coupled device (CCD) for detecting the emission of fluorescence intensity from an RT-cPCR mix in a microliter volume glass capillary. The measured results from the image-processing scheme indicate that the RT-cPCR prototype with a CCD-based fluorometer can achieve similar DNA quantification reproducibility compared to commercial machines, even when the initial DNA concentration in the test PCR mix is reduced to 10 copies/μL To assess the performance of the prototype, a single DNA template, HBV 122 base pairs, with known concentrations and a single labeling dye, SYBR Green I, was used in the PCR mixes undergoing the same thermal cycling in both the prototype and commercial RT-PCR machines for comparing their measured and predicted fluorescence intensities emitted from the glass capillaries.	./cache/cord-350890-ajxvjkmq.txt	./txt/cord-350890-ajxvjkmq.txt