id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349745-zlhu1jit	Konrad, Regina	Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020	2020-03-05		.txt	text/plain	2417	137	55	The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We found that the SARS-CoV E gene screening assay with the QuantiTect Virus +Rox Vial kit showed moderate to high amounts of unspecific signals in late cycles in 61% (451/743) of the tested patient samples and also of negative extraction and non-template controls (Table, Figure 2 ), which complicated the evaluation of the qPCR result. The Public Health Microbiology Laboratory in Bavaria was confronted with SARS-CoV-2-related events very early: once the assays and control materials arrived and the PCR assays were performed for the first time, a large contact investigation around the first German COVID-19 patient (data not shown) was immediately started, with so far more than 700 samples.	./cache/cord-349745-zlhu1jit.txt	./txt/cord-349745-zlhu1jit.txt