id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346989-604gho1u	Chen-Harris, Haiyin	Ultra-deep mutant spectrum profiling: improving sequencing accuracy using overlapping read pairs	2013-02-12		.txt	text/plain	7125	334	49	RESULTS: We demonstrate that overlapping read pairs (ORP) -generated by combining short fragment sequencing libraries and longer sequencing reads -significantly reduce sequencing error rates and improve rare variant detection accuracy. We demonstrate the novel use of mismatch rates in the overlapping read pairs (ORP) to provide an unbiased assessment of the sequencer-derived quality scores when selecting a read filtering threshold, as well as to estimate position-dependent sequencing errors without relying on a clonal control. ORPs reduce error rates and provide benchmarks for quality scores For all five samples, 3 natural viral and 2 plasmid controls, Illumina paired-end sequencing was carried out using relatively short sequencing fragment libraries combined with relatively long reads (112 bp) to generate overlapping read pairs (ORP).	./cache/cord-346989-604gho1u.txt	./txt/cord-346989-604gho1u.txt