id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-346436-p61mpc6t	Onodera, Kenji	Selection for 3′-End Triplets for Polymerase Chain Reaction Primers	2007		.txt	text/plain	3709	225	72	Over 2000 primer sequences from successful PCR experiments used with varieties of templates and conditions were analyzed for finding frequencies of the 3′-end triplets. This chapter discusses a trend in 3′-end triplet frequencies in primers used in successful PCR experiments and proposes requirements for the 3′-end of a primer. From the VirOligo database, 2137 PCR primer sequences were retrieved for detailed analysis of the 3 -end triplets of successful PCR primers (8; see Note 1). The analysis of the 3 -end triplets of primers (8; see Fig. 5 and Table 1) showed all 64 types were used in successful PCR experiments from the VirOligo database. 3. When primers are obtained by a primer design program, the user needs to note that some primer search settings such as 3 -end "GC clamp" interfere with the 3 -end triplet selection.	./cache/cord-346436-p61mpc6t.txt	./txt/cord-346436-p61mpc6t.txt