id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-345475-ttrcmtu4	de Oliveira, Luisa Abruzzi	Reference Genes for the Normalization of Gene Expression in Eucalyptus Species	2011-12-24		.txt	text/plain	9744	595	54	Given the increasing interest in the functional genomics of Eucalyptus and the need for validated reference genes for a broader set of species and experimental conditions, we sought to identify the most stably expressed genes in a set of 21,432 genes assayed by microarray developed to compare stem vascular (xylem) and leaf tissues of E. According to the NormFinder analysis of gene expression in leaves, xylem tissues and among species, the stability values of the 15 genes studied were <0.138, with error bars no greater than 0.044 ( Fig. 3C ; Table 3 ). When we analyzed the gene expression in all tissues/organs and species, the stability value was in the range between 0.017 and 0.106, proving again that all genes elected are good references for RT-qPCR studies in Eucalyptus. By RT-qPCR, the expression stability of eight of the 50 best candidate genes selected by SAM and SDMA was addressed in different organs (leaves and flowers) and vascular tissues (xylem) derived from six species of Eucalyptus.	./cache/cord-345475-ttrcmtu4.txt	./txt/cord-345475-ttrcmtu4.txt