id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-344745-sgkq1l93	Selim, Karim	Molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in Egypt during 2012	2013-11-18		.txt	text/plain	2757	172	56	title: Molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in Egypt during 2012 The aim of this study aimed to survey the Egyptian chicken field for infectious bronchitis virus and study the genomic differentiation between isolated field samples seeking the new variant strain emerged in the Egyptian field. For virus isolation, the supernatants of IBV-positive selected 13 samples determined by RT-PCR were inoculated into five specific pathogen free embryonated chicken eggs (KoumOshiem SPF chicken farm, Fayoum, Egypt) 10-day-old for each sample. The genetic analysis of 100 amino acids sequence from position 263-362 of SP1 gene for the selected 13 Egyptian viruses was done and the hypervariable region of SP1 gene showed multiple mutations as shown in Table 4 in comparison with variant-2 strain. S1 gene sequence analysis of a nephro-pathogenic strain of avian infectious bronchitis virus in Egypt	./cache/cord-344745-sgkq1l93.txt	./txt/cord-344745-sgkq1l93.txt