id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342568-3sj235rm	Bald-Blume, Niklas	Development of a molecular assay for the general detection of tospoviruses and the distinction between tospoviral species	2017-02-11		.txt	text/plain	5364	277	54	In this study a new method for plant virus diagnosis is described using the Luminex xTAG technology to test for tospoviruses in general and for the four species TSWV, WSMoV, INSV and CaCV. Infected, dried plant material of 12 tospoviral isolates from eight different species was obtained from the DSMZ including single isolates of alstroemeria necrotic streak virus (ANSV), CaCV, GRSV, IYSV, TCSV and WSMoV as well as three isolates each of INSV and TSWV. The generic tospovirus primers Tospo_GENs/as (without tags) allowed the detection of all eight tospoviruses and of all three isolates of INSV and TSWV tested in RT-PCR experiments. A molecular assay for the detection of tospoviruses in general and for viruses from the four species belonging to this genus (TSWV, INSV, CaCV and WSMoV), using the Luminex xTAG technology, was successfully developed.	./cache/cord-342568-3sj235rm.txt	./txt/cord-342568-3sj235rm.txt