id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-341141-bgrgzfoo	Hou, Peili	Rapid detection of infectious bovine Rhinotracheitis virus using recombinase polymerase amplification assays	2017-12-13		.txt	text/plain	4693	234	51	In this study, we described the development of a lateral flow dipstrip (LFD) of isothermal recombinase polymerase amplification (RPA) method for rapid detection of IBRV. The assay performance on acute-phase high fever clinical samples collected from cattle with no vaccine against IBRV, which were suspected to be infected with IBRV, was validated by detecting 24 fecal, 36 blood, 38 nasal swab and 8 tissue specimens, and compared with SYBR Green I based real-time PCR. The initial agarose gel result showed that Primer set 4-2F/4-2R/ 4-2LF yielded specific amplification efficiency for the RPA assay, and produced the expected size of the product was 250 base-pairs (Fig. 1a) , while the primers/probe targeting glycoprotein gB of the IBRV genome in this study could not be used to amplify effectively in the initial screen (data not show).	./cache/cord-341141-bgrgzfoo.txt	./txt/cord-341141-bgrgzfoo.txt