id author title date pages extension mime words sentences flesch summary cache txt cord-339656-u0cpklsv de Groot-Mijnes, Jolanda D.F. Identification of New Pathogens in the Intraocular Fluid of Patients With Uveitis 2010-11-30 .txt text/plain 4012 243 44 Methods Ocular fluids from 139 patients suspected of infectious uveitis, but negative for herpes simplex virus, varicella-zoster virus, cytomegalovirus, and Toxoplasma gondii by polymerase chain reaction and/or antibody analysis in intraocular fluids, were assessed for the presence of 18 viruses and 3 bacteria by real-time polymerase chain reaction (PCR). The remainders of ocular fluid samples from patients with PCR-and/or GWC-confirmed infectious uveitis (ocular toxoplasmosis, n ϭ 13; HSV anterior uveitis, n ϭ 10; rubella virus-associated Fuchs heterochromic uveitis syndrome (FHUS), n ϭ 14) and from patients with cataract in the absence of intraocular inflammation (n ϭ 11) served as controls. • NUCLEIC ACID ISOLATION AND REAL-TIME PCR: The ocular fluid samples were analyzed for the presence of adenovirus, EBV, HHV6, Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia trachomatis DNA and of coronaviruses 229E, OC43, and NL63, enteroviruses, human metapneumovirus, influenza A and B virus, parainfluenza virus 1 to 4, HPeV, respiratory syncytial virus A and B, and rubella virus RNA. ./cache/cord-339656-u0cpklsv.txt ./txt/cord-339656-u0cpklsv.txt