id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-339419-b6tr2zyx	Lee, Thomas Ming-Hung	DNA-based bioanalytical microsystems for handheld device applications	2006-01-18		.txt	text/plain	5425	331	49	Recent progresses in the miniaturization of various biological processing steps for the sample preparation, DNA amplification (polymerase chain reaction), and product detection are delineated in detail. The organization of the following contents is based upon the three basic DNA processing modules of sample preparation, target amplification, and product detection. Thermal lysis can be easily adapted to microfabricated amplification systems as the initial high-temperature step (∼95 • C) employed to denature the double-stranded (ds) DNA template is powerful enough to open up the cell membranes [10, 12, 19] . The sensing protocol basically involves the immobilization of an oligonucleotide onto a transducer surface, and upon the hybridization of complementary target sequence, the binding event is detected by optical, microgravimetric (mass-sensitive), or electrochemical methods. To further increase the sensitivity of the gold nanoparticle-based assay, a signal amplification step Pictorial representation of the working principle of the molecular beacon-type capture probe labeled with ferrocene group for the reagentless sequence-specific DNA detection.	./cache/cord-339419-b6tr2zyx.txt	./txt/cord-339419-b6tr2zyx.txt