id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-338205-sy91rnse	Li, Chenxi	Laboratory Diagnosis of Coronavirus Disease-2019 (COVID-19)	2020-07-02		.txt	text/plain	7515	436	51	With limited understanding of COVID-19, it is difficult to exclude SARS-CoV-2 infection based on a single negative PCR result, especially when testing was used for upper respiratory tract specimens. The study found that SARS-CoV-2 could be detected in all primer-probe sets applied in the qRT-PCR tests, but significant discrepancy was observed in the detection limit and the ability to identify negatives and positives with a lower viral load. Compared with the qRT-PCR kit, nested RT-PCR analysis showed higher sensitivity and specificity, indicating that it is more suitable for clinical application to detect SARS-CoV-2 in cases with low viral load. In cases where RT-PCR assays are negative and there is a strong epidemiological link to SARS-CoV-2 infection, paired serum samples (in the acute and convalescent-phase) could support diagnosis once validated serology tests are available with the initial samples collected in the first week of COVID-19 and the second collected after 2-4 weeks [28] .	./cache/cord-338205-sy91rnse.txt	./txt/cord-338205-sy91rnse.txt