id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335784-v7nbck0n	Barak, N.	Lessons from applied large-scale pooling of 133,816 SARS-CoV-2 RT-PCR tests	2020-10-20		.txt	text/plain	3136	202	57	Pooling multiple swab samples prior to RNA extraction and RT-PCR analysis was proposed as a strategy to reduce costs and increase throughput of SARS-CoV-2 tests. Key open questions concern reduced sensitivity due to sample dilution; the rate of false positives; the actual efficiency (number of tests saved by pooling) and the impact of infection rate in the population on assay performance. Major diagnostic challenges have emerged, mainly, the need for high throughput SARS-CoV-2 RT-PCR tests, aimed to detect not only symptomatic but also asymptomatic infectious viral carriers and to screen special or at-risk populations (such as health care personnel or nursing home tenants), in order to contain viral spread and guide control measures. To our knowledge, this is the most extensive analysis, addressing key considerations of efficiency, sensitivity and feasibility in the actual reality of routine, large-scale implementation of sample pooling for SARS-CoV-2 detection.	./cache/cord-335784-v7nbck0n.txt	./txt/cord-335784-v7nbck0n.txt