id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335085-7pxkhgbq	Dessau, R. B.	Coronaviruses in spinal fluid of patients with acute monosymptomatic optic neuritis	2009-01-29		.txt	text/plain	2145	130	64	Material and methods ‐ Spinal fluids from 37 patients with AMON and 15 surgical control patients with protrusion of the intervertebral disk were assayed with a nested multiplex polymerase chain reaction with primers specific for human coronaviruses strain (HCV) 229E and OC43. To investigate the possibility of an infection with human coronaviruses (HCV) in early MS and as a possible cause of AMON we have analyzed cerebrospinal fluid (CSF) from patients with AMON using reverse transcriptase reaction and the polymerase chain reaction (RT-PCR) applying primers specific for HCV. CSF from 4 patients and 1 control ( Table 2) were positive on nested RT-PCR using the HCV-229E primers and all samples were negative with the HCV-OC43 primers. HCV-229E RNA was found in the CSF by RT-PCR in 4 of 37 patients with AMON and in 1 of 15 controls.	./cache/cord-335085-7pxkhgbq.txt	./txt/cord-335085-7pxkhgbq.txt