id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-333261-knj2rrut	Albright, Catherine J.	An exercise in molecular epidemiology: Human rhinovirus prevalence and genetics	2011-11-11		.txt	text/plain	3408	205	59	To facilitate the collections of HRV sequences over a number of years, a virology experiment was designed in which students test nasal lavage samples to look for HRV infection. The extensive data available on HRV genomes enables many bioinformatics opportunities for students, including alignment of genome sequences to look for mutations at the RNA level and differences among protein sequences. Students can examine differences in the HRV serotypes over several years of data regarding a university population to identify HRV mutations that have occurred and their severity in causing symptoms in the host. In this inquiry-based laboratory project, students use a variety of techniques, including RNA isolation, cDNA synthesis, qPCR, and agarose gel electrophoresis to look for the presence of HRV in nasal lavage samples from human subjects. By analyzing surveys in which subjects indicate severity of their symptoms, stress factors, and average hours of rest per night, students can identify possible contributors to HRV infection.	./cache/cord-333261-knj2rrut.txt	./txt/cord-333261-knj2rrut.txt