id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332522-adul9nzf	Wu, Qingfa	Development of Taqman RT-nested PCR system for clinical SARS-CoV detection	2004-04-02		.txt	text/plain	2810	143	62	In this study, 12 sets of nested primers covering the SARS-CoV genome have been screened and showed sufficient sensitivity to detect SARS-CoV in RNA isolated from virus cultured in Vero 6 cells. To optimize further the reaction condition of those nested primers sets, seven sets of nested primers have been chosen to compare their reverse transcribed efficiency with specific and random primers, which is useful to combine RT with the first round of PCR into a one-step RT-PCR. Through investigations on a test panel of whole blood obtained from 30 SARS patients and 9 control persons, the specificity and sensitivity of the Taqman RT-nested PCR system was found to be 100 and 83%, respectively, which suggests that the method is a promising one to diagnose SARS in early stages. To compare the sensitivities of these 12 sets of nested primers, serial 10-fold di-lution genome cDNA of BJ01 that reverse transcribed with random primer was used as the template to carry out the nested PCR.	./cache/cord-332522-adul9nzf.txt	./txt/cord-332522-adul9nzf.txt