id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332024-jk983q4p	Briese, Thomas	Diagnostic System for Rapid and Sensitive Differential Detection of Pathogens	2005-02-17		.txt	text/plain	1738	84	36	We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences and report its application for detecting 22 respiratory pathogens in clinical samples. We describe a diagnostic system for rapid, sensitive, multiplex discrimination of microbial gene sequences and report its application for detecting 22 respiratory pathogens in clinical samples. To address the need for sensitive multiplex assays in diagnostic molecular microbiology, we created a polymerase chain reaction (PCR) platform in which microbial gene targets are coded by a library of 64 distinct Masscode tags (Qiagen Masscode technology, Qiagen, Hilden, Germany). Multiplex primer sets were designed to identify up to 22 respiratory pathogens in a single Mass Tag PCR reaction; sensitivity was established by using synthetic DNA and RNA standards as well as titered viral stocks; the utility of Mass Tag PCR was determined in blinded analysis of previously diagnosed clinical specimens.	./cache/cord-332024-jk983q4p.txt	./txt/cord-332024-jk983q4p.txt