id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331932-oujdl459	Lung, O.	Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens	2015-12-12		.txt	text/plain	6362	293	44	The user‐friendly assay detected and differentiated between four viruses [porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus, porcine circovirus type 2, porcine respiratory corona virus], four bacteria (Mycoplasma hyopneumoniae, Pasteurella multocida, Salmonella enterica serovar Choleraesuis, Streptococcus suis), and further differentiated between type 1 and type 2 PRRSV as well as toxigenic and non‐toxigenic P. Here, a microarray assay with associated multiplex RT-PCRs for detection and differentiation of four viruses and four bacteria involved in PRDC using a novel user-friendly electronic microarray in which capture probe printing, hybridization, washing and reporting are fully integrated and automated is described. Similarly, representative whole-genome sequences, as well as full and partial sequences of homologous genes from related and unrelated non-targets such as TGEV, porcine circovirus type 1 (PCV1), as well as other Salmonella enterica serovars, and Mycoplasma species were downloaded for in silico analysis of probe specificity. The analytical specificity of the viral and bacterial multiplex PCR assays was assessed by amplifying panels of 14 non-target viruses and 21 bacteria, respectively (Table 3) .	./cache/cord-331932-oujdl459.txt	./txt/cord-331932-oujdl459.txt