id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-331616-arnuoufn	Blank, Walter A.	Virus PCR Assay Panels: An Alternative to the Mouse Antibody Production Test	2004		.txt	text/plain	3515	159	37	The authors compare MAP testing with PCR-based detection methods, focusing on differences in animal use, laboratory requirements, sample size, and limits of detection. Until recently, the mouse antibody production (MAP) test was the primary method of screening for viruses of murine origin 6 ( Table 1) , but the application of modern molecular biology methods to this purpose presents certain advantages. Because PCR amplifies only DNA molecules, one detects viruses with RNA samples from the animals and test them for virus-specific antibodies using the enzymelinked immunosorbent (ELISA), indirect fluorescent antibody (IFA), or hemagglutination inhibition (HAI) assays. To prevent false-positive results due to contamination with PCR templates, reagent preparation, sample processing, and PCR amplification/product detection should all take place in separate laboratories. Comparison of the sensitivity of in vivo antibody production tests with in vitro PCR-based methods to detect infectious contamination of biological materials	./cache/cord-331616-arnuoufn.txt	./txt/cord-331616-arnuoufn.txt