id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-329517-3yn80r9h	Yang, Jin-Long	Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo	2009-09-15		.txt	text/plain	3564	192	55	title: Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo The objective of this study was to develop a fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast and accurate quantification of GPV DNA in infected goslings, which can aid in the understanding of the regular distribution pattern and the nosogenesis of GPV in vivo. Ten-fold dilutions of the pVP3 plasmid DNA were tested by the established FQ-PCR assay to evaluate the sensitivity of the system, and the detection limit was found to be 2.8 × 10 1 copies/reaction. Viral load quantification using the established FQ-PCR demonstrated that the GPV DNA copy number of each sample could be calculated using the cycle threshold (Ct) value determined from the standard curve. In conclusion, the established real-time PCR assay was rapid, sensitive, and specific for the detection and quantification of GPV DNA.	./cache/cord-329517-3yn80r9h.txt	./txt/cord-329517-3yn80r9h.txt