id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-328961-waxtb759	Pratelli, Annamaria	PCR assay for the detection and the identification of atypical canine coronavirus in dogs	2002-10-01		.txt	text/plain	1889	95	57	Comparative sequence analysis of the PCR products of the M gene and fragments of the pol1a and pol1b genes of canine coronavirus (CCoV) have demonstrated that two separate clusters of CCoV are present in dogs. The sequence analysis of the PCR products of the M and S genes carried out on the faecal samples from the two pups confirmed that the FCoV-like CCoV had caused the disease (personal observations). On the basis of these preliminary results, a PCR assay was developed to detect and identify the FCoV-like CCoV strains from faecal samples of infected dogs. In a previous study, similar nucleotide substitutions in the binding site of the internal primer CCoV3 used for the n-PCR were demonstrated in the sequence analysis of the PCR products from five faecal samples of pups with diarrhoea.	./cache/cord-328961-waxtb759.txt	./txt/cord-328961-waxtb759.txt