id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325736-gs9d8y55	Marin, J	Persistence of Viruses in Upper Respiratory Tract of Children with Asthma	2000-07-31		.txt	text/plain	1874	135	61	title: Persistence of Viruses in Upper Respiratory Tract of Children with Asthma Conclusions: The persistent presence of viruses in the upper respiratory tract of asthmatic children shows a possible connection between viral infections and asthma. 4 In this study nasopharyngeal swabs were taken from asthmatic children whose asthma was well controlled, and when they were at least 3 weeks free of any respiratory infections. The samples were examined for the presence of adenovirus DNA and rhinovirus and coronavirus RNA. Five microlitres of c-DNA product were subsequently amplified in 50µl PCR reaction mixture consisting of 10 reaction buffer, 25 mM MgCl 2 , 20 mM dNTP, 5 U/l of DNA Taq polymerase (all reagents provided by Perkin Elmer, New Jersey, USA) and 50 µM of each specific primer for rhinoviruses and coronaviruses (TIB MOLBIOL, Berlin, Germany) (Table I) . found that upper respiratory tract viruses were associated with over 80% of asthma exacerbations in children.	./cache/cord-325736-gs9d8y55.txt	./txt/cord-325736-gs9d8y55.txt