id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325529-pid58g2r	Ben-Ami, Roni	Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection	2020-06-23		.txt	text/plain	2822	158	50	METHODS: We tested the efficiency and sensitivity of pooling strategies for RNA extraction and RT-PCR detection of SARS-CoV-2. Implementing the 8-sample Dorfman pooling to test 26,576 samples from asymptomatic individuals, we identified 31 (0.12%) SARS-CoV-2 positive samples, achieving a 7.3-fold increase in throughput. Some key constrains are (1) a limit on the number of stages due to the importance of delivering a test result quickly, exemplified by the urgent clinical context of COVID-19 diagnosis; (2) a limit on the ability to dilute samples and still safely identify a single positive sample in a pool; and (3) favorability of simple algorithms which may minimize human error in a laboratory setting. Specifically, we have demonstrated that pooling lysates from 5 or 8 nasopharyngeal swab samples retains sufficient sensitivity of viral RNA detection, allowing identification of SARS-CoV-2-positive individuals, while increasing throughput 5-fold to 7.5-fold.	./cache/cord-325529-pid58g2r.txt	./txt/cord-325529-pid58g2r.txt