id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325101-9qslo6qh	Gizzi, Aline Baumann da Rocha	Presence of infectious agents and co-infections in diarrheic dogs determined with a real-time polymerase chain reaction-based panel	2014-01-16		.txt	text/plain	5156	239	50	Although many pathogens have been individually detected with real-time polymerase chain reaction (PCR), a comprehensive panel of agents that cause diarrhea in privately owned dogs has not yet been established. Therefore, the aim of this study was to investigate pathogenic co-infections in populations of diarrheic and control owned dogs using a real-time PCR analysis of a panel of diarrhea-causing agents. The most prevalent agent involved in co-infections was canine parvovirus type 2 (CPV-2), and 21/36 (58.3%) of the diarrheic samples positive for CPV-2 were associated with others agents, most commonly with Clostridium perfringens alpha toxin (CPA), Cryptosporidium spp., and Giardia spp. The detection of individual pathogens in the panel with real-time PCR (Table 3) showed that CPA was the most prevalent pathogen in the fecal samples, infecting 40/104 (38.5%) diarrheic dogs and 6/43 (14.0%) control dogs, and the difference between the groups was highly statistically significant (P = 0.006).	./cache/cord-325101-9qslo6qh.txt	./txt/cord-325101-9qslo6qh.txt