id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-323963-whv88ggl	Fan, Xiaofeng	Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples	2006-08-11		.txt	text/plain	5713	303	51	Among RNA templates, hepatitis C virus (HCV) represents an excellent example to challenge the potential of LRP technology due to its extensive secondary structures and its difficulty to be readily cultured in vitro. Thus, our LRP protocol could be applied for the amplification of other difficult RNA templates and may facilitate RNA virus research such as linked viral mutations and reverse genetics. In some experiments, we mixed a RT enzyme with Pfu DNA polymerase (Stratagene), a similar strategy as used in long PCR, to improve full-length cDNA synthesis [8] . A representative neighbor-joining (NJ) tree constructed based on HCV E1 domain of 20 clones derived from 9.1 kb LRP product, which was amplified using mixed serum from samples LIV19 and LIV23. A refined long RT-PCR technique to amplify complete viral RNA genome sequences from clinical samples: Application to a novel hepatitis C virus variant of genotype 6	./cache/cord-323963-whv88ggl.txt	./txt/cord-323963-whv88ggl.txt