id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-322391-tumpiid5	Freymuth, François	Detection of viral, Chlamydia pneumoniae and Mycoplasma pneumoniae infections in exacerbations of asthma in children	1999-08-31		.txt	text/plain	3813	190	47	According to the virus, a viral isolation technique, immunofluorescence assays (IFA) or both were used for the detection of rhinovirus, enterovirus, respiratory syncytial (RS) virus, adenovirus, coronavirus 229E, influenza and parainfluenza virus. Results: Using IFA and viral isolation techniques, viruses were detected in 33.3% of cases, and by PCR techniques, nucleic acid sequences of virus, Chlamydia pneumoniae and Mycoplasma pneumoniae were obtained in 71.9% of cases. We also previously reported that molecular techniques were more sensitive than IFA and viral isolation for the detection of RS virus, rhinovirus, adenovirus and parainfluenza virus in nasal specimens collected from infants with bronchiolitis (Freymuth et al., 1997) , and the present study further confirmed that observation. Respiratory viruses, Chlamydia pneumoniae and Mycoplasma pneumoniae alone or associated, were detected in 81.8% of asthmatic exacerbations, with rhinovirus and RS virus being the most frequent isolated agents.	./cache/cord-322391-tumpiid5.txt	./txt/cord-322391-tumpiid5.txt