id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319970-1gu0a6cb	Edin, Alicia	Development and Laboratory Evaluation of a Real-Time PCR Assay for Detecting Viruses and Bacteria of Relevance for Community-Acquired Pneumonia	2015-03-13		.txt	text/plain	5049	238	39	title: Development and Laboratory Evaluation of a Real-Time PCR Assay for Detecting Viruses and Bacteria of Relevance for Community-Acquired Pneumonia Diagnostic accuracy of the qPCR assay varied between 60% positive agreement with standard tests for Streptococcus pneumoniae and 100% for Mycoplasma pneumoniae, Moraxella catarrhalis, and Staphylococcus aureus. To verify that the method could detect viruses in sputum, sputum specimens each with a volume of 350 mL were spiked with100 mL from a NpA specimen positive for either RSV or influenza A before nucleic acid extraction and qPCR assay analysis were performed as described in the sections DNA and RNA Extraction and Primers Probes and qPCR Conditions, respectively. In the evaluation of the assay performance for detection of virus in sputum, we found that sputum specimens spiked with influenza A-or RSV-containing NpA specimens were positive at 25.9 AE 0.6 (n Z 5) and 27.7 AE 1.1 (n Z 5) cycles, respectively.	./cache/cord-319970-1gu0a6cb.txt	./txt/cord-319970-1gu0a6cb.txt