id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319685-dw0qsl4s	Porter, Emily	Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis	2014-04-25		.txt	text/plain	5690	272	58	Recent evidence suggests that a mutation in the spike protein gene of feline coronavirus (FCoV), which results in an amino acid change from methionine to leucine at position 1058, may be associated with feline infectious peritonitis (FIP). Tissue and faecal samples collected post mortem from cats diagnosed with or without FIP were subjected to RNA extraction and quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) to detect FCoV RNA. Data evaluating FCoV relative copy numbers in tissue and faecal samples from cats with and without FIP were analysed using a multilevel modelling approach (MLwiN v2.27) [25] , to account for the repeated measures within cats, and a non-parametric Mann-Whitney U test. Moreover, the majority (77%) of FCoV RNA sequences in faecal samples from cats with FIP had a methionine codon at position 1058 in the FCoV S protein gene, suggesting that these animals were shedding an enteric form of the virus.	./cache/cord-319685-dw0qsl4s.txt	./txt/cord-319685-dw0qsl4s.txt