id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-319392-zg7gkf0j	Yi, Li	Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs	2011-11-18		.txt	text/plain	3494	200	59	title: Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). The phylogenetic analysis of the hemagglutinin gene of the local wild-type CDV strains revealed that the seven local isolates all belonged to the Asia-1 lineage, and were clustered closely with one another at the same location. The CDV cDNA products were further simultaneously amplified by a combined two-step RT-PCR assay using two primer sets to distinguish the vaccine and field strains. A multiplex reverse transcriptionnested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus	./cache/cord-319392-zg7gkf0j.txt	./txt/cord-319392-zg7gkf0j.txt