id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-317129-wa1j2f6b	Zhang, Jia	De Novo synthesis of PCR templates for the development of SARS diagnostic assay	2003		.txt	text/plain	2319	117	58	This highly efficient and safe strategy for obtaining SARS gene fragments is useful for the development of PCR assays, as well as for the preparation of reliable positive controls for PCR testing kits. This single-step sequential primer extension was expected to yield mainly final templates with no intermediate products. As shown in Figure 1 , DNA fragments in lengths of 182 and 204 bp were obtained from primers of set A and set B, respectively, by the single-step sequential primer extension where each reaction contained four partially overlapping a The expected products were extended from reverse primers (AR or BR) to the first forward primers. De novo synthesis of the PCR template complimentary to a viral genome provides a tool for the rapid development of early diagnostic assays for any new pathogen as soon as its sequence is known.	./cache/cord-317129-wa1j2f6b.txt	./txt/cord-317129-wa1j2f6b.txt