id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316932-fia1w9jt	Ireland, D. C.	Improved detection of rhinoviruses in nasal and throat swabs by seminested RT‐PCR	2005-12-07		.txt	text/plain	4099	232	61	PCR tests in which a primary "touchdown" PCR was followed by secondary reactions using PV or HRV specific primers were able to differentiate HRVs of 48 serotypes from EVs. PVnRT‐PCR and HRVnRT‐PCR were then used to test nasal and throat swabs from adult subjects with naturally acquired respiratory virus infections. 2"PCRs using a primer of sequence A together with primers complementary to either B (HRVnRT-PCR) or C (PVnRT-PCR) are then used to differentiate between HRVs and other PVs. We have applied this test directly to nasal and throat swabs from controls and from individuals with naturally acquired colds and compared the rate of PV detection by PVnRT-PCR and by cell culture. This study has shown that PVnRT-PCR is a t least five times more sensitive than cell culture for the detection of PVs in nasal and throat swabs.	./cache/cord-316932-fia1w9jt.txt	./txt/cord-316932-fia1w9jt.txt