id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316309-8xe7cg8q	Lee, Wah Heng	LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens	2008-09-10		.txt	text/plain	5895	296	53	We build on our previous paper [14] describing in further detail the AES algorithm that identifies genomics sequences that can be successfully amplified by random primers, facilitating the design of appropriate microarray probes for detection of the pathogen. In our paper, we reported an observation that experiments using random priming amplification often resulted in incomplete hybridization of the pathogen genome marked by interspersed genomic regions not detected by tiling probes on the microarray (Figure 1. Since our pathogen detection chip contains tiling 40-mer probes of both RSV and HMPV, the number and distribution of the probes with high signal intensities would give a good indication of the amount of PCR products generated across the target genome by a tagged-random primer. We expect that a tagged-random primer with desirable amplification efficiency that generates sufficient PCR products uniformly across the whole target genome would result in high signal intensity probes distributed evenly across the whole genome.	./cache/cord-316309-8xe7cg8q.txt	./txt/cord-316309-8xe7cg8q.txt