id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316295-x636ux34	Roth, Bernhard	Isolation of influenza viruses in MDCK 33016PF cells and clearance of contaminating respiratory viruses	2012-01-11		.txt	text/plain	4140	190	44	Abstract This paper summarizes results obtained by multiplex PCR screening of human clinical samples for respiratory viruses and corresponding data obtained after passaging of virus-positive samples in MDCK 33016PF cells. Using lower inoculum dilutions than those normally applied for preparations containing influenza virus (total dilution of the original sample of ∼104), the positive results for the different viruses turned negative already after 2 or 3 passages in MDCK 33016PF cells. In a similar way, samples with positive and questionable multiplex PCR results only for viruses other than influenza virus were also cultivated for 2 or 3 passages in MDCK 33016PF cells. Considering the selection of specimens, the high percentage of influenza-positive results is not surprising, but a significant number of samples (66/370 or 17.8%) also tested positive for other viruses, such as adenovirus, bocavirus, coronavirus, enterovirus, metapneumovirus (HMPV), parainfluenza virus (PIV), rhinovirus, and respiratory syncytial virus (RSV).	./cache/cord-316295-x636ux34.txt	./txt/cord-316295-x636ux34.txt