id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-316173-ocdlh310	LIU, Dafei	One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus	2018-01-23		.txt	text/plain	997	48	56	title: One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10(2.1) TCID(50) for CDV, 10(1.9) TCID(50) for CPV and 10(3) copies for CaKoV. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs. The viral DNA/RNA of fecal samples collected from dogs with clinical symptoms of diarrhea were extracted and tested in parallel with both the one-step multiplex PCR/RT-PCR and the commercial Rapid CDV/CPV Ag Test Kit (Bionote, Gyeonggi, Republic of Korea) for CDV or CPV, according to the manufacturer's instruction, and a traditional simplex RT-PCR for CaKoV, as described previously with minor modification [4] . Detection of canine distemper virus in dogs by real-time RT-PCR	./cache/cord-316173-ocdlh310.txt	./txt/cord-316173-ocdlh310.txt