id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315780-uhi66unn	Paton, David	Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus	1997-07-31		.txt	text/plain	2905	149	53	Abstract An RT-PCR method was developed that amplified genetic material from the 5′ end of the S protein gene of both transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV), but discriminated between the two by the size of the product generated. Detection of TGEV in clinical specimens was possible using a spin column method to extract RNA and sensitivity was compared to virus isolation and antigen detection ELISA. There are also reports of the use of DNA probes and of RT-PCR as detectors of TGEV RNA (Bae et al., 1991; Vaughn et al., 1996; Wesley et al., 1991; Jackwood et al., 1995) but the methods were not shown to be suitable for the direct detection of virus in clinical samples. Differentiation of transmissible gastroenteritis virus from porcine respiratory coronavirus and other antigenically related coronaviruses by using cDNA probes specific for the 5' region of the S glycoprotein gene	./cache/cord-315780-uhi66unn.txt	./txt/cord-315780-uhi66unn.txt