id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315541-tirod4t6	Henriques, Ana Margarida	Development and validation of a real-time PCR for the detection and quantification of porcine circovirus type 2	2018-07-17		.txt	text/plain	3552	167	51	This paper reports the development and validation of a real-time PCR targeted to ORF1 and based on a TaqMan probe for the detection of porcine circovirus type 2 DNA in swine samples. The real-time PCR method described in this paper was performed with DNA from this strain, and an amplification curve with Ct 19.9 was obtained, confirming that such mutation in the probe annealing sequence had no effect in the reaction. The test performed with a plasmid containing the fragment to be amplified in the qPCR, with known The DNA samples used for the determination of the intra-and inter-assay variabilities for medium Ct values was not the same due to the lack of available DNA Fig. 2 Amplification curves obtained in the real-time PCR reaction performed for the determination of the limit of detection.	./cache/cord-315541-tirod4t6.txt	./txt/cord-315541-tirod4t6.txt