id author title date pages extension mime words sentences flesch summary cache txt cord-315476-7rdiesav Peret, Teresa C. T. Characterization of Human Metapneumoviruses Isolated from Patients in North America 2002-06-01 .txt text/plain 1959 119 54 In this study, 11 isolates from 10 patients with respiratory disease from Quebec, Canada, were tested by a reverse-transcriptase polymerase chain reaction based on the fusion protein gene. In this study, 11 isolates from 10 patients with respiratory disease from Quebec, Canada, were tested by a reverse-transcriptase polymerase chain reaction based on the fusion protein gene. In the present article, we describe polymerase chain reaction (PCR) and sequencing studies done on 11 isolates from respiratory specimens from 10 Canadian patients with acute respiratory tract illness. Published nucleocapsid (N) and fusion (F) gene sequences of HMPV and avian pneumovirus were used to develop primers for detection and sequencing of HMPV at the Respiratory Virus Section (Centers for Disease Control and Prevention, Atlanta). We detected virus in isolates from children with acute respiratory tract infection, as described in the first report of HMPV [1] . ./cache/cord-315476-7rdiesav.txt ./txt/cord-315476-7rdiesav.txt