id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312456-6lxc2rj2	Soltan, Mohamed A.	Comparison of electron microscopy, ELISA, real time RT-PCR and insulated isothermal RT-PCR for the detection of Rotavirus group A (RVA) in feces of different animal species	2016-05-11		.txt	text/plain	4209	218	53	title: Comparison of electron microscopy, ELISA, real time RT-PCR and insulated isothermal RT-PCR for the detection of Rotavirus group A (RVA) in feces of different animal species The aim of this investigation was to evaluate a commercially available RT-iiPCR assay for RVA detection in feces from different animal species. Therefore, the aim of our investigation was to evaluate a recently available insulated isothermal RT-PCR (RT-iiPCR) reagent set (POCKIT TM Rotavirus A Reagent Set, GeneReach USA, Lexington, MA, USA) with use of a portable PCR machine, which could potentially be used for point-of-need detection for RVA in the feces of different animal species. Additionally, the sensitivity of the rotavirus RT-iiPCR reagent set was evaluated by comparison with the commercially available rtRT-PCR assay using 10-fold serial dilutions of nucleic acid extracted from a positive bovine clinical sample. There was a significant difference in the number of positive samples detected with the in-house rtRT-PCR assay versus the other two molecular tests.	./cache/cord-312456-6lxc2rj2.txt	./txt/cord-312456-6lxc2rj2.txt