id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312161-egwo19oc	Aw, Tiong Gim	Detection of pathogens in water: from phylochips to qPCR to pyrosequencing	2011-12-05		.txt	text/plain	4551	209	31	Microbial water quality monitoring has undergone tremendous transition in recent years, with novel molecular tools beginning to offer rapid, high-throughput, sensitive and specific detection of a wide spectrum of microbial pathogens that challenge traditional culture-based techniques. High-density microarrays, quantitative real-time PCR (qPCR) and pyrosequencing which are considered to be breakthrough technologies borne out of the 'molecular revolution' are at present emerging rapidly as tools of pathogen detection and discovery. The limitations in detecting and identifying pathogens directly from environmental water samples by culture or microscopy can now be addressed by integrating concentration techniques with molecular tools to provide sensitive, specific and quantitative data on any pathogens of interest. Pyrosequencing technology is revolutionizing the study of microbial ecology as well as direct metagenomic detection Detection of pathogens in water Aw and Rose 425 High levels of several classes of resistance genes in bacterial communities exposed to antibiotic were identified.	./cache/cord-312161-egwo19oc.txt	./txt/cord-312161-egwo19oc.txt