id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-312139-g1hczx54	Liu, Wei	Non-specific Primers Reveal False-negative Risk in Detection of COVID-19 Infections	2020-04-11		.txt	text/plain	3347	212	60	Based on the primers provided by research institutes from different countries, especially primers from China, detailed analysis of non-specificity of primer sequences had been conducted, and interference of human mRNA targeted by the primer was discussed deeply for RT-PCR detection of COVID-19 infections. https://doi.org/10.1101/2020.04.07.20056804 doi: medRxiv preprint 8 Although many patients tested one or more negative before receiving positive results for SARS-CoV-2 virus in China, it was difficult to understand the extent to which this abnormal phenomenon occurs. To eliminate the host interference in RT-PCR detection of COVID-19 infections, specific amplification of the intended target of SARS-Cov-2 sequence required that primers matched as little as possible to any human RNA transcript. Hence it was not difficult to draw a conclusion that non-specificity of the primer designed for SARS-CoV-2 virus might be an important factor resulting in so many false-negative diagnoses for COVID-19 infections in China.	./cache/cord-312139-g1hczx54.txt	./txt/cord-312139-g1hczx54.txt